Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0013399

Physical Location & Seq

Genomic Coordinates Forward (+)

18348691 .. 18351371

Gene Structure 2681 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1200200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1983 bp

ATGAAACCCCGCCACCACCTGAGACTCCTCCGCCTTACCAAACCCACGCCCTCAACCACCACGTGCCGCCACTATGCCACAAAGTACACGGCCAAAATAACCTCAACCTCCCCAACCGGCCTTTCTGTCTCGGCCGAGGTAACCCCGCCGCCACCCCTCCCCACGGACATCCGCGGCTACGCTCTCCCGCGGCGCGACCTCGTCTGCAAAGCCACCCAAATCCTCCTGGGCTGCCAATCCCCTTCCGCCATGTTATCAGACCCCTTCTCCGACCTCTCTGACTACCTCACCTCCCTCTCCCTCCACATCACCCCGTCTGAAGCCTCCGAAATCCTGAAATCGGTAACCTGCCCCGACCTCGCCGTCAAATTCTTCCGATTTTGCCCCTCGCTCTCCCCTAATTTCCACCACGACGCCTTCACCTGCAACCGAATCCTCCTCATCCTCTCCAAGTCCACCTCCCCCGCCCGGTTCGACCTAGTCCGGTCGATCCTCCGCGACATGGACCGCTCCAGCATAAGTGGCAACATCTCCACCGTCAACATCCTCATCGGCCTGTTCGGCAGCACCGAAGACTTGGAAACCTGTATTGGGTTGGTGAAGAAATGGAATCTCAACATGAACAGCTACACTTACAAGTGCTTGCTTCAGGCCTTCCTGCGCTCTCGCGATTCCGGCAAAGCTTTCGATACTTACATCGACATGCGGCGGCGGGGTTACAAGCTCGACATTTTTGCTTACAACATGCTGCTGGATGCATTGGCAAAAGAGGAAAAGGTTGAGGATGCACGCAAGGTGTTTGATGATATGAAGAAGAAGCATTGCGAGCCGGATGAGTTTACTTACACGATTATGATCAGAATGGTTGGGAAATTGGGCAAGGGCGATGAGTGTCGCGGGTTGTTTCAGGAGATGGTGAACAAGGGTTGCGCTCCGAATATGATGTTTTATAATACTATGATTCAGGCTCTTGCTCGGAGCAAGATGGTTGACAAGGCCATCATTGTGTTCTCGAAAATGGTACAGAACAATTGCCGGCCTAATGAATTTACGTACAGTGTGGTTTTGAATCTTTTGGTTGCAGAAGGGCAGCTGGGTAGGTTTGATGAGGTGGTGGGAATGTCGAAGAAGTACATGACGAAGTCGATATATGCATATCTTGTGAGGACATTGAGTAAATTGGGTCATGCCGGGGAAGCTCACAGGTTGTTCTGCAACATGTGGAGCTTCCATGACATGGGGGATCGGGATGCATATACTTCAATGTTGGAGAGTTTGTGCAGTGCGGGTAAAACTGCAGAGGCGGTTGACATGCTAAGTAACATTCATGAGAAAGGGATAACTACTGATACTATCATGTACAACACAGTTTTGGCAACTCTTGGAAAGTTGAAGCAGATACCCCATCTTCATGAACTGTACGAAAAGATGAAACTTGATGGGCCTGAACCGGACATATTTACCTACAACATTATGATTTCGAGCTTTGGTAGAGCTGGGAAGGTTCATGAGGCTGTGACGATATTCGAACATCTGGAGAATAGTGATTGTAAACCGGATATTATCTCTTACAATTCGTTGATCAATTGCCTTGGGAAAAATGGTGATGTGGATGAAGCCCACATGAGGTTTAAGGAGATGCAAGAGAAGGGTTTTAGCCCTGATGTCGTCACCTACAGCACTCTGATCGAGTGCTTTGGCAAGACGGATAGGGTTGAAATGGCATGCAGGCTGTTTGACGACATGCTTGCTCAAGGCTGCTGTCCGAATATAGTGACATACAACATCTTGCTTGATTGTCTTGAGAGGTGTGGGAGGACTGCTGAGGCAGTGGATCTATATGCGAAACTTAAGCAGCAAGGGTTGACACCGGATTGGATTACATACGCAGTGCTTGAACGGTTGCAGAGCGGTTCGCATAGGAGAGTTAGGGTTCGTAGGCAGAGTCCGATCACAGGTTGGGTTGTGAGCCCTTTAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0033993 GO:0034641 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050896 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:1901360 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

661

Amino Acids

74.59

Weight (kDa)

8.55

Isoelectric Point (pI)

44.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	208 - 256	2.3e-10	PPR repeat family
PPR_long	PF17177	212 - 373	8.5e-20	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	234 - 291	4.4e-12	Pentatricopeptide repeat domain
TPR_24	PF23276	236 - 339	5.6e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	238 - 271	1.3e-06	PPR repeat
PPR_2	PF13041	243 - 288	1e-17	PPR repeat family
PPR	PF01535	246 - 275	3.7e-06	PPR repeat
PPR	PF01535	280 - 310	1.3e-06	PPR repeat
PPR_3	PF13812	301 - 360	5.6e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	308 - 341	3.5e-06	PPR repeat
PPR_2	PF13041	312 - 359	6.1e-14	PPR repeat family
PPR	PF01535	317 - 345	4.2e-06	PPR repeat
PPR_2	PF13041	450 - 496	8.4e-10	PPR repeat family
PPR_long	PF17177	473 - 634	8.6e-11	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	473 - 534	2.7e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	481 - 511	1.5e-09	PPR repeat
PPR_2	PF13041	484 - 531	7.9e-13	PPR repeat family
PPR	PF01535	487 - 517	3.1e-06	PPR repeat
TPR_24	PF23276	497 - 616	2.4e-09	Fungal tetratrico peptide repeats
PPR_1	PF12854	516 - 547	1.7e-09	PPR repeat
PPR_2	PF13041	519 - 567	2.9e-17	PPR repeat family
PPR	PF01535	522 - 552	5.6e-09	PPR repeat
PPR_3	PF13812	544 - 599	4e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	550 - 582	4.7e-09	PPR repeat
PPR_2	PF13041	554 - 601	2.1e-16	PPR repeat family
PPR	PF01535	557 - 587	3.2e-06	PPR repeat
PPR_3	PF13812	577 - 632	1.9e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	586 - 613	5.1e-07	PPR repeat
PPR_2	PF13041	589 - 632	3.5e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013399)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51965
fragaria_vesca	FvH4_6g32590
malus_domestica	MD09G1200200.v1.1
prunus_persica	Prupe.3G056800_v2.0.a1
pyrus_communis	pycom09g11780 pycom15g36760
rosa_chinensis	RchiOBHm_Chr2g0141041
rosa_laevigata	RLG00000019910
rosa_multiflora	Rmu_sc0004769.1_g000016 Rmu_sc0012897.1_g000006
rosa_roxburghii	Rroxscaffold_2G00104260
rosa_rugosa	Rorug02G0366300
rosa_samantha	Rh2AG417700 Rh2BG427600 Rh2CG404000 Rh2DG437100
rosa_wichuraiana	Rw2G034190 Rw2G034250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	951
AarI	CACCTGC	1 cut(s)	431
AasI	GACNNNNNNGTC	1 cut(s)	362
Acc36I	ACCTGC	2 cut(s)	356, 431
AccB7I	CCANNNNNTGG	1 cut(s)	1237
AccBSI	CCGCTC	2 cut(s)	510, 1911
AccII	CGCG	6 cut(s)	174, 190, 195, 498, 669, 897
AclWI	GGATC	3 cut(s)	484, 1251, 1842
AcoI	YGGCCR	2 cut(s)	90, 132
AcsI	RAATTY	2 cut(s)	368, 1046
AcuI	CTGAAG	2 cut(s)	339, 632
AcvI	CACGTG	1 cut(s)	63
AcyI	GRCGYC	1 cut(s)	414
AdeI	CACNNNGTG	1 cut(s)	63
AfaI	GTAC	6 cut(s)	86, 1023, 1055, 1133, 1361, 1421
AfiI	CCNNNNNNNGG	8 cut(s)	163, 340, 468, 502, 591, 1237, 1626, 1955
AflII	CTTAAG	1 cut(s)	1850
AflIII	ACRYGT	1 cut(s)	1218
AgsI	TTSAA	5 cut(s)	1069, 1263, 1393, 1718, 1898
AjnI	CCWGG	1 cut(s)	225
AjuI	GAANNNNNNNTTGG	4 cut(s)	106, 138, 573, 605
AluBI	AGCT	8 cut(s)	627, 683, 724, 1093, 1199, 1227, 1485, 1496
AluI	AGCT	8 cut(s)	627, 683, 724, 1093, 1199, 1227, 1485, 1496
Alw26I	GTCTC	2 cut(s)	16, 133
AlwI	GGATC	3 cut(s)	484, 1251, 1842
AoxI	GGCC	8 cut(s)	90, 118, 132, 553, 651, 996, 1037, 1442
ApeKI	GCWGC	6 cut(s)	231, 564, 748, 1090, 1758, 1855
ApoI	RAATTY	2 cut(s)	368, 1046
AspLEI	GCGC	3 cut(s)	195, 663, 932
AspS9I	GGNCC	2 cut(s)	505, 1442
AsuC2I	CCSGG	2 cut(s)	469, 1192
AsuHPI	GGTGA	7 cut(s)	280, 301, 412, 610, 928, 1616, 1663
AsuII	TTCGAA	1 cut(s)	1527
AvaII	GGWCC	1 cut(s)	505
BanII	GRGCYC	1 cut(s)	1973
BbrPI	CACGTG	1 cut(s)	63
BbsI	GAAGAC	1 cut(s)	579
BbvCI	CCTCAGC	1 cut(s)	1824
BbvI	GCAGC	6 cut(s)	218, 576, 735, 1102, 1745, 1867
BccI	CCATC	5 cut(s)	907, 979, 1007, 1413, 1433
BceAI	ACGGC	2 cut(s)	105, 347
BcgI	CGANNNNNNTGC	8 cut(s)	667, 701, 716, 750, 1669, 1703, 1730, 1764
BciT130I	CCWGG	1 cut(s)	227
BclI	TGATCA	2 cut(s)	855, 1581
BcnI	CCSGG	2 cut(s)	469, 1192
BcoDI	GTCTC	2 cut(s)	16, 133
BfaI	CTAG	1 cut(s)	479
BfmI	CTRYAG	2 cut(s)	1296, 1675
BfrI	CTTAAG	1 cut(s)	1850
BfuAI	ACCTGC	2 cut(s)	356, 431
BglI	GCCNNNNNGGC	1 cut(s)	561
Bme1390I	CCNGG	3 cut(s)	227, 469, 1192
Bme18I	GGWCC	1 cut(s)	505
BmgT120I	GGNCC	2 cut(s)	505, 1442
BmrFI	CCNGG	3 cut(s)	227, 469, 1192
BmsI	GCATC	4 cut(s)	745, 775, 1240, 1629
BpiI	GAAGAC	1 cut(s)	579
BpmI	CTGGAG	2 cut(s)	496, 1556
Bpu10I	CCTNAGC	1 cut(s)	1824
Bpu14I	TTCGAA	1 cut(s)	1527
BpuEI	CTTGAG	2 cut(s)	1737, 1823
BpuMI	CCSGG	2 cut(s)	469, 1192
BsaAI	YACGTR	2 cut(s)	63, 1053
BsaBI	GATNNNNATC	1 cut(s)	854
BsaHI	GRCGYC	1 cut(s)	414
BsaJI	CCNNGG	7 cut(s)	135, 162, 172, 188, 226, 1191, 1591
BsaWI	WCCGGW	4 cut(s)	483, 1450, 1555, 1870
BsaXI	ACNNNNNCTCC	6 cut(s)	251, 275, 281, 305, 1262, 1292
Bsc4I	CCNNNNNNNGG	8 cut(s)	163, 340, 468, 502, 591, 1237, 1626, 1955
Bse118I	RCCGGY	2 cut(s)	116, 1035
Bse3DI	GCAATG	1 cut(s)	820
Bse8I	GATNNNNATC	1 cut(s)	854
BseBI	CCWGG	1 cut(s)	227
BseDI	CCNNGG	7 cut(s)	135, 162, 172, 188, 226, 1191, 1591
BseGI	GGATG	8 cut(s)	168, 441, 543, 760, 790, 838, 1255, 1618
BseJI	GATNNNNATC	1 cut(s)	854
BseLI	CCNNNNNNNGG	8 cut(s)	163, 340, 468, 502, 591, 1237, 1626, 1955
BseMI	GCAATG	1 cut(s)	820
BseMII	CTCAG	2 cut(s)	11, 1815
BseRI	GAGGAG	2 cut(s)	17, 428
BseX3I	CGGCCG	1 cut(s)	132
BseXI	GCAGC	6 cut(s)	218, 576, 735, 1102, 1745, 1867
BseYI	CCCAGC	2 cut(s)	1093, 1496
BsgI	GTGCAG	1 cut(s)	1300
Bsh1236I	CGCG	6 cut(s)	174, 190, 195, 498, 669, 897
Bsh1285I	CGRYCG	2 cut(s)	135, 488
BshFI	GGCC	8 cut(s)	92, 120, 134, 555, 653, 998, 1039, 1444
BsiEI	CGRYCG	2 cut(s)	135, 488
BslI	CCNNNNNNNGG	8 cut(s)	163, 340, 468, 502, 591, 1237, 1626, 1955
BsmAI	GTCTC	2 cut(s)	16, 133
BsnI	GGCC	8 cut(s)	92, 120, 134, 555, 653, 998, 1039, 1444
Bsp119I	TTCGAA	1 cut(s)	1527
Bsp1286I	GDGCHC	1 cut(s)	1973
Bsp1407I	TGTACA	1 cut(s)	1359
Bsp143I	GATC	7 cut(s)	489, 855, 1243, 1581, 1686, 1834, 1950
Bsp68I	TCGCGA	1 cut(s)	669
BspANI	GGCC	8 cut(s)	92, 120, 134, 555, 653, 998, 1039, 1444
BspCNI	CTCAG	2 cut(s)	12, 1816
BspFNI	CGCG	6 cut(s)	174, 190, 195, 498, 669, 897
BspHI	TCATGA	3 cut(s)	1327, 1411, 1507
BspMAI	CTGCAG	1 cut(s)	1300
BspMI	ACCTGC	2 cut(s)	356, 431
BspPI	GGATC	3 cut(s)	484, 1251, 1842
BspT104I	TTCGAA	1 cut(s)	1527
BspTI	CTTAAG	1 cut(s)	1850
BsrBI	CCGCTC	2 cut(s)	510, 1911
BsrDI	GCAATG	1 cut(s)	820
BsrFI	RCCGGY	2 cut(s)	116, 1035
BsrGI	TGTACA	1 cut(s)	1359
BssAI	RCCGGY	2 cut(s)	116, 1035
BssECI	CCNNGG	7 cut(s)	135, 162, 172, 188, 226, 1191, 1591
BssMI	GATC	7 cut(s)	489, 855, 1243, 1581, 1686, 1834, 1950
BssNI	GRCGYC	1 cut(s)	414
BssT1I	CCWWGG	1 cut(s)	1591
Bst2UI	CCWGG	1 cut(s)	227
Bst4CI	ACNGT	5 cut(s)	538, 1058, 1369, 1419, 1902
BstACI	GRCGYC	1 cut(s)	414
BstAFI	CTTAAG	1 cut(s)	1850
BstAUI	TGTACA	1 cut(s)	1359
BstBAI	YACGTR	2 cut(s)	63, 1053
BstBI	TTCGAA	1 cut(s)	1527
BstC8I	GCNNGC	7 cut(s)	644, 790, 827, 1037, 1726, 1730, 1749
BstDEI	CTNAG	3 cut(s)	20, 1316, 1824
BstDSI	CCRYGG	3 cut(s)	162, 172, 188
BstEII	GGTNACC	2 cut(s)	139, 343
BstF5I	GGATG	8 cut(s)	168, 441, 543, 760, 790, 838, 1255, 1618
BstFNI	CGCG	6 cut(s)	174, 190, 195, 498, 669, 897
BstHHI	GCGC	3 cut(s)	195, 663, 932
BstKTI	GATC	7 cut(s)	492, 858, 1246, 1584, 1689, 1837, 1953
BstMAI	GTCTC	2 cut(s)	16, 133
BstMBI	GATC	7 cut(s)	489, 855, 1243, 1581, 1686, 1834, 1950
BstMCI	CGRYCG	2 cut(s)	135, 488
BstMWI	GCNNNNNNNGC	5 cut(s)	522, 561, 675, 826, 1196
BstNI	CCWGG	1 cut(s)	227
BstNSI	RCATGY	6 cut(s)	706, 748, 1222, 1315, 1728, 1747
BstPI	GGTNACC	2 cut(s)	139, 343
BstSCI	CCNGG	3 cut(s)	225, 467, 1190
BstSFI	CTRYAG	2 cut(s)	1296, 1675
BstSNI	TACGTA	1 cut(s)	1053
BstUI	CGCG	6 cut(s)	174, 190, 195, 498, 669, 897
BstV1I	GCAGC	6 cut(s)	218, 576, 735, 1102, 1745, 1867
BstV2I	GAAGAC	1 cut(s)	579
BstX2I	RGATCY	1 cut(s)	1834
BstYI	RGATCY	1 cut(s)	1834
BstZI	CGGCCG	1 cut(s)	132
BsuRI	GGCC	8 cut(s)	92, 120, 134, 555, 653, 998, 1039, 1444
BtgI	CCRYGG	3 cut(s)	162, 172, 188
BtgZI	GCGATG	1 cut(s)	900
BtsCI	GGATG	8 cut(s)	168, 441, 543, 760, 790, 838, 1255, 1618
BtsI	GCAGTG	3 cut(s)	1288, 1836, 1896
BtsIMutI	CAGTG	4 cut(s)	1063, 1288, 1836, 1896
BtuMI	TCGCGA	1 cut(s)	669
BveI	ACCTGC	2 cut(s)	356, 431
Cac8I	GCNNGC	7 cut(s)	644, 790, 827, 1037, 1726, 1730, 1749
CciI	TCATGA	3 cut(s)	1327, 1411, 1507
CfoI	GCGC	3 cut(s)	195, 663, 932
Cfr10I	RCCGGY	2 cut(s)	116, 1035
Cfr13I	GGNCC	2 cut(s)	505, 1442
Cfr42I	CCGCGG	2 cut(s)	175, 191
CseI	GACGC	1 cut(s)	422
Csp6I	GTAC	6 cut(s)	85, 1022, 1054, 1132, 1360, 1420
CviQI	GTAC	6 cut(s)	85, 1022, 1054, 1132, 1360, 1420
DdeI	CTNAG	3 cut(s)	20, 1316, 1824
DpnI	GATC	7 cut(s)	491, 857, 1245, 1583, 1688, 1836, 1952
DpnII	GATC	7 cut(s)	489, 855, 1243, 1581, 1686, 1834, 1950
DraIII	CACNNNGTG	1 cut(s)	63
DrdI	GACNNNNNNGTC	1 cut(s)	362
DseDI	GACNNNNNNGTC	1 cut(s)	362
EaeI	YGGCCR	2 cut(s)	90, 132
EagI	CGGCCG	1 cut(s)	132
EciI	GGCGGA	2 cut(s)	20, 235
EclXI	CGGCCG	1 cut(s)	132
Eco105I	TACGTA	1 cut(s)	1053
Eco130I	CCWWGG	1 cut(s)	1591
Eco147I	AGGCCT	1 cut(s)	653
Eco24I	GRGCYC	1 cut(s)	1973
Eco47I	GGWCC	1 cut(s)	505
Eco52I	CGGCCG	1 cut(s)	132
Eco57I	CTGAAG	2 cut(s)	339, 632
Eco72I	CACGTG	1 cut(s)	63
Eco91I	GGTNACC	2 cut(s)	139, 343
EcoO65I	GGTNACC	2 cut(s)	139, 343
EcoRII	CCWGG	1 cut(s)	225
EcoT14I	CCWWGG	1 cut(s)	1591
EcoT22I	ATGCAT	3 cut(s)	760, 1156, 1255
EcoT38I	GRGCYC	1 cut(s)	1973
ErhI	CCWWGG	1 cut(s)	1591
FauI	CCCGC	7 cut(s)	17, 153, 195, 472, 705, 890, 1279
FbaI	TGATCA	2 cut(s)	855, 1581
FokI	GGATG	8 cut(s)	155, 428, 530, 767, 797, 845, 1262, 1625
FriOI	GRGCYC	1 cut(s)	1973
FspBI	CTAG	1 cut(s)	479
GlaI	GCGC	3 cut(s)	194, 662, 931
GsaI	CCCAGC	2 cut(s)	1097, 1500
GsuI	CTGGAG	2 cut(s)	496, 1556
HaeIII	GGCC	8 cut(s)	92, 120, 134, 555, 653, 998, 1039, 1444
HgaI	GACGC	1 cut(s)	422
HhaI	GCGC	3 cut(s)	195, 663, 932
Hin1I	GRCGYC	1 cut(s)	414
Hin6I	GCGC	3 cut(s)	193, 661, 930
HinP1I	GCGC	3 cut(s)	193, 661, 930
HincII	GTYRAC	4 cut(s)	541, 991, 1309, 1866
HindII	GTYRAC	4 cut(s)	541, 991, 1309, 1866
HindIII	AAGCTT	1 cut(s)	681
HinfI	GANTC	7 cut(s)	24, 432, 610, 671, 961, 1069, 1945
HphI	GGTGA	7 cut(s)	280, 301, 412, 610, 928, 1616, 1663
Hpy166II	GTNNAC	9 cut(s)	87, 456, 541, 840, 919, 991, 1309, 1553, 1866
Hpy8I	GTNNAC	9 cut(s)	87, 456, 541, 840, 919, 991, 1309, 1553, 1866
Hpy99I	CGWCG	1 cut(s)	416
HpyAV	CCTTC	7 cut(s)	252, 274, 427, 664, 1079, 1495, 1642
HpyCH4III	ACNGT	5 cut(s)	538, 1058, 1369, 1419, 1902
HpyCH4IV	ACGT	2 cut(s)	62, 1052
HpyF10VI	GCNNNNNNNGC	5 cut(s)	522, 561, 675, 826, 1196
HpyF3I	CTNAG	3 cut(s)	20, 1316, 1824
HpySE526I	ACGT	2 cut(s)	62, 1052
Hsp92I	GRCGYC	1 cut(s)	414
HspAI	GCGC	3 cut(s)	193, 661, 930
KroI	GCCGGC	1 cut(s)	1035
KroNI	GCCGGC	1 cut(s)	1037
Ksp22I	TGATCA	2 cut(s)	855, 1581
KspI	CCGCGG	2 cut(s)	175, 191
Kzo9I	GATC	7 cut(s)	489, 855, 1243, 1581, 1686, 1834, 1950
LmnI	GCTCC	4 cut(s)	515, 937, 978, 1224
Lsp1109I	GCAGC	6 cut(s)	218, 576, 735, 1102, 1745, 1867
LweI	GCATC	4 cut(s)	745, 775, 1240, 1629
MaeI	CTAG	1 cut(s)	479
MaeII	ACGT	2 cut(s)	62, 1052
MaeIII	GTNAC	7 cut(s)	139, 343, 716, 1319, 1516, 1669, 1774
MalI	GATC	7 cut(s)	491, 857, 1245, 1583, 1688, 1836, 1952
MbiI	CCGCTC	2 cut(s)	510, 1911
MboI	GATC	7 cut(s)	489, 855, 1243, 1581, 1686, 1834, 1950
MboII	GAAGA	7 cut(s)	364, 584, 613, 823, 826, 1138, 1400
MfeI	CAATTG	2 cut(s)	1030, 1585
MflI	RGATCY	1 cut(s)	1834
MhlI	GDGCHC	1 cut(s)	1973
MluCI	AATT	8 cut(s)	368, 400, 872, 1030, 1046, 1178, 1573, 1585
MlyI	GAGTC	2 cut(s)	18, 1954
MmeI	TCCRAC	2 cut(s)	294, 1248
Mph1103I	ATGCAT	3 cut(s)	760, 1156, 1255
MroNI	GCCGGC	1 cut(s)	1035
MseI	TTAA	3 cut(s)	1632, 1851, 1976
MslI	CAYNNNNRTG	4 cut(s)	701, 851, 1004, 1410
MspA1I	CMGCKG	3 cut(s)	174, 190, 1093
MspCI	CTTAAG	1 cut(s)	1850
MspR9I	CCNGG	3 cut(s)	227, 469, 1192
MunI	CAATTG	2 cut(s)	1030, 1585
MvaI	CCWGG	1 cut(s)	227
MvnI	CGCG	6 cut(s)	174, 190, 195, 498, 669, 897
MwoI	GCNNNNNNNGC	5 cut(s)	522, 561, 675, 826, 1196
NaeI	GCCGGC	1 cut(s)	1037
NciI	CCSGG	2 cut(s)	469, 1192
NdeII	GATC	7 cut(s)	489, 855, 1243, 1581, 1686, 1834, 1950
NgoMIV	GCCGGC	1 cut(s)	1035
NmeAIII	GCCGAG	2 cut(s)	110, 160
NmuCI	GTSAC	3 cut(s)	1516, 1669, 1774
NruI	TCGCGA	1 cut(s)	669
NsiI	ATGCAT	3 cut(s)	760, 1156, 1255
NspI	RCATGY	6 cut(s)	706, 748, 1222, 1315, 1728, 1747
NspV	TTCGAA	1 cut(s)	1527
PaeI	GCATGC	1 cut(s)	1728
PagI	TCATGA	3 cut(s)	1327, 1411, 1507
PaqCI	CACCTGC	1 cut(s)	431
PceI	AGGCCT	1 cut(s)	653
PciI	ACATGT	1 cut(s)	1218
PcsI	WCGNNNNNNNCGW	1 cut(s)	567
PdiI	GCCGGC	1 cut(s)	1037
PfeI	GAWTC	5 cut(s)	432, 610, 671, 961, 1069
PflFI	GACNNNGTC	3 cut(s)	200, 479, 1141
PflMI	CCANNNNNTGG	1 cut(s)	1237
PleI	GAGTC	2 cut(s)	18, 1953
PmaCI	CACGTG	1 cut(s)	63
PmlI	CACGTG	1 cut(s)	63
PpsI	GAGTC	2 cut(s)	18, 1953
Ppu21I	YACGTR	2 cut(s)	63, 1053
PscI	ACATGT	1 cut(s)	1218
PsiI	TTATAA	1 cut(s)	951
Psp6I	CCWGG	1 cut(s)	225
PspCI	CACGTG	1 cut(s)	63
PspEI	GGTNACC	2 cut(s)	139, 343
PspFI	CCCAGC	2 cut(s)	1093, 1496
PspGI	CCWGG	1 cut(s)	225
PspPI	GGNCC	2 cut(s)	505, 1442
PstI	CTGCAG	1 cut(s)	1300
PsuI	RGATCY	1 cut(s)	1834
PsyI	GACNNNGTC	3 cut(s)	200, 479, 1141
PvuII	CAGCTG	1 cut(s)	1093
RruI	TCGCGA	1 cut(s)	669
RsaI	GTAC	6 cut(s)	86, 1023, 1055, 1133, 1361, 1421
RsaNI	GTAC	6 cut(s)	85, 1022, 1054, 1132, 1360, 1420
RseI	CAYNNNNRTG	4 cut(s)	701, 851, 1004, 1410
SacII	CCGCGG	2 cut(s)	175, 191
SaqAI	TTAA	3 cut(s)	1632, 1851, 1976
Sau3AI	GATC	7 cut(s)	489, 855, 1243, 1581, 1686, 1834, 1950
Sau96I	GGNCC	2 cut(s)	505, 1442
SchI	GAGTC	2 cut(s)	18, 1954
ScrFI	CCNGG	3 cut(s)	227, 469, 1192
SduI	GDGCHC	1 cut(s)	1973
SfaNI	GCATC	4 cut(s)	745, 775, 1240, 1629
SfcI	CTRYAG	2 cut(s)	1296, 1675
Sfr303I	CCGCGG	2 cut(s)	175, 191
SfuI	TTCGAA	1 cut(s)	1527
SgrBI	CCGCGG	2 cut(s)	175, 191
SinI	GGWCC	1 cut(s)	505
SmiMI	CAYNNNNRTG	4 cut(s)	701, 851, 1004, 1410
SmlI	CTYRAG	3 cut(s)	1752, 1802, 1850
SmoI	CTYRAG	3 cut(s)	1752, 1802, 1850
SnaBI	TACGTA	1 cut(s)	1053
SphI	GCATGC	1 cut(s)	1728
Sse9I	AATT	8 cut(s)	368, 400, 872, 1030, 1046, 1178, 1573, 1585
SseBI	AGGCCT	1 cut(s)	653
SspMI	CTAG	1 cut(s)	479
StuI	AGGCCT	1 cut(s)	653
StyD4I	CCNGG	3 cut(s)	225, 467, 1190
StyI	CCWWGG	1 cut(s)	1591
TaaI	ACNGT	5 cut(s)	538, 1058, 1369, 1419, 1902
TaiI	ACGT	2 cut(s)	65, 1055
TasI	AATT	8 cut(s)	368, 400, 872, 1030, 1046, 1178, 1573, 1585
TatI	WGTACW	3 cut(s)	84, 1131, 1359
TauI	GCSGC	6 cut(s)	69, 151, 177, 193, 709, 712
TfiI	GAWTC	5 cut(s)	432, 610, 671, 961, 1069
Tru1I	TTAA	3 cut(s)	1632, 1851, 1976
Tru9I	TTAA	3 cut(s)	1632, 1851, 1976
TscAI	CASTG	4 cut(s)	1063, 1288, 1836, 1896
TseFI	GTSAC	3 cut(s)	1516, 1669, 1774
TseI	GCWGC	6 cut(s)	231, 564, 748, 1090, 1758, 1855
Tsp45I	GTSAC	3 cut(s)	1516, 1669, 1774
TspGWI	ACGGA	2 cut(s)	179, 1721
TspRI	CASTG	4 cut(s)	1063, 1288, 1836, 1896
Tth111I	GACNNNGTC	3 cut(s)	200, 479, 1141
Van91I	CCANNNNNTGG	1 cut(s)	1237
Vha464I	CTTAAG	1 cut(s)	1850
VpaK11BI	GGWCC	1 cut(s)	505
XapI	RAATTY	2 cut(s)	368, 1046
XceI	RCATGY	6 cut(s)	706, 748, 1222, 1315, 1728, 1747
XspI	CTAG	1 cut(s)	479
Zsp2I	ATGCAT	3 cut(s)	760, 1156, 1255

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.