pycom15g36760

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013399
Physical Location & Seq
Forward (+)
36711651 .. 36712931
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g36760.1

Sequence Viewer

Length: 1164 bp
ATGTTATCAGACCCCTTCTCCGAACTCTCTGACTGCCTCACCTCCCTCTCCCTCCACATCACCCCGTCCGAAGCCTCCGAAATCCTGAAATCGGTAACCTGCCCCGACCTCGCCGTCAAATTCTTCCGATTTTGCCCCTCTCTCTCCTCTAATTTCCACCACGACGCCTTCACCTGCAGTCGAATCCTCCTCATCCTCTCCAAGTCCACCTCCCCCGCCCGGTTCGACCTAGTCCGGTCGATCCTCCGCGACATGGACCGCTCCAGCATACATGGCAACATCTCCACCGTCAACATGCTTATCGGCCTGTTCGGCAGCACCGAAGACTTGGAAACCTGTATTGGGTTGGTGAAGAAATGGAATCTCAACATGAACAGCTACACTTACAAGTGCTTGCTTCAGGCCTTCCTGCGCTCTCGCGATTCCGGCAAAGCTCTCGATACTTACATCGACATGCGGCGGCGGGGTAACAAGCACGACATTTTTGCCTACAACATGCTGCTGGATGCGTTGGCGAACGACGAAAAGGTTGAGGATGCACGCAAGGTGTGTGATGATATGAAGAAGAAGCATTGCGAGCCGGATGAGTTTACGTACACGATTATGATCAGAATGGTTGGGAAATTGGGTAAGGGCGATGAGTGTCTCGGGTTGTTTCAGGAGATGGTGAACAAGGGTTGCGCTCCGAATACGATGCTTTATAATACTATGATTCAGGCTCTTGCTCGGAGCAAGATGGTTGACAAGGCCATCATTGTGTTCTCGAAAATGGTACAGAACCATTGCCGGCCTAATGAATTTACGTACAGTGTGGTTTTGAATCTTTTGGTTGCAGAAGGGCAGCTGGGTAGGTTTGATGAGGTGGTGGGGATGTCGAAGAAGTACATGACGAAGTCAATATATGCATATCTTGTGAGGACATTGAGTAAATTGGGTCATGCCGGGGAAGCTCACAGGTTGTTCTGCAACATGTGGAGCTTCCATGACATGGGGGATCGGGATGCATATACTTCAATGTTGGAGAGTTTGTGCAGTGCAGGTAGAACTGCAGAGGCGGTTGACTTGCTAAATAACATTCATGAGAAAGGGATAACTACCGATACTATTATGTACAACAGTTTTGGCAGCTCATGGAAAGTTGAAGCAGATACCCCATCTTCATGA

Protein Analysis

388

Amino Acids

43.64

Weight (kDa)

6.82

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 125 - 173 1.9e-09 PPR repeat family
PPR_long PF17177 129 - 290 1.1e-17 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 151 - 208 9e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 160 - 205 6.4e-16 PPR repeat family
PPR PF01535 197 - 227 2.2e-07 PPR repeat
PPR_3 PF13812 218 - 277 8.7e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 225 - 258 4.7e-07 PPR repeat
PPR_2 PF13041 229 - 276 3.5e-14 PPR repeat family
PPR PF01535 234 - 262 5e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013399)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51965
fragaria_vesca FvH4_6g32590
malus_domestica MD09G1200200.v1.1
prunus_persica Prupe.3G056800_v2.0.a1
pyrus_communis pycom09g11780 pycom15g36760
rosa_chinensis RchiOBHm_Chr2g0141041
rosa_laevigata RLG00000019910
rosa_multiflora Rmu_sc0004769.1_g000016 Rmu_sc0012897.1_g000006
rosa_roxburghii Rroxscaffold_2G00104260
rosa_rugosa Rorug02G0366300
rosa_samantha Rh2AG417700 Rh2BG427600 Rh2CG404000 Rh2DG437100
rosa_wichuraiana Rw2G034190 Rw2G034250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
AarI CACCTGC 1 cut(s) 182
AasI GACNNNNNNGTC 1 cut(s) 113
Acc36I ACCTGC 3 cut(s) 107, 182, 1028
AccB7I CCANNNNNTGG 1 cut(s) 988
AccBSI CCGCTC 1 cut(s) 261
AccII CGCG 2 cut(s) 249, 420
AciI CCGC 7 cut(s) 216, 247, 259, 457, 460, 463, 1055
AclWI GGATC 2 cut(s) 235, 1002
AcsI RAATTY 2 cut(s) 119, 797
AcuI CTGAAG 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 5 cut(s) 596, 774, 806, 884, 1112
AfiI CCNNNNNNNGG 5 cut(s) 91, 219, 253, 342, 988
AflIII ACRYGT 1 cut(s) 969
AgsI TTSAA 3 cut(s) 820, 1014, 1142
AjuI GAANNNNNNNTTGG 2 cut(s) 324, 356
AluBI AGCT 6 cut(s) 378, 434, 844, 950, 978, 1128
AluI AGCT 6 cut(s) 378, 434, 844, 950, 978, 1128
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 2 cut(s) 235, 1002
Ama87I CYCGRG 1 cut(s) 647
AoxI GGCC 4 cut(s) 304, 402, 747, 788
ApeKI GCWGC 4 cut(s) 315, 499, 841, 1125
ApoI RAATTY 2 cut(s) 119, 797
AspLEI GCGC 2 cut(s) 414, 683
AspS9I GGNCC 1 cut(s) 256
AsuC2I CCSGG 2 cut(s) 220, 943
AsuHPI GGTGA 5 cut(s) 31, 52, 163, 361, 679
AvaI CYCGRG 1 cut(s) 647
AvaII GGWCC 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 330
BbvI GCAGC 4 cut(s) 327, 486, 853, 1137
BccI CCATC 4 cut(s) 658, 730, 758, 1162
BceAI ACGGC 1 cut(s) 98
BcgI CGANNNNNNTGC 6 cut(s) 418, 452, 467, 501, 676, 710
BclI TGATCA 1 cut(s) 606
BcnI CCSGG 2 cut(s) 220, 943
BcoDI GTCTC 1 cut(s) 650
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 2 cut(s) 175, 1047
BfuAI ACCTGC 3 cut(s) 107, 182, 1028
BglI GCCNNNNNGGC 1 cut(s) 312
BisI GCNGC 6 cut(s) 316, 458, 461, 500, 842, 1126
BlsI GCNGC 6 cut(s) 317, 459, 462, 501, 843, 1127
Bme1390I CCNGG 2 cut(s) 220, 943
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 256
BmrFI CCNGG 2 cut(s) 220, 943
BmsI GCATC 4 cut(s) 496, 526, 684, 991
BpiI GAAGAC 1 cut(s) 330
BpmI CTGGAG 1 cut(s) 247
BpuMI CCSGG 2 cut(s) 220, 943
BsaAI YACGTR 2 cut(s) 594, 804
BsaBI GATNNNNATC 1 cut(s) 605
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 942
BsaWI WCCGGW 1 cut(s) 234
BsaXI ACNNNNNCTCC 3 cut(s) 32, 1013, 1043
Bsc4I CCNNNNNNNGG 5 cut(s) 91, 219, 253, 342, 988
Bse118I RCCGGY 1 cut(s) 786
Bse3DI GCAATG 2 cut(s) 571, 781
Bse8I GATNNNNATC 1 cut(s) 605
BseDI CCNNGG 1 cut(s) 942
BseGI GGATG 6 cut(s) 192, 511, 541, 589, 876, 1006
BseJI GATNNNNATC 1 cut(s) 605
BseLI CCNNNNNNNGG 5 cut(s) 91, 219, 253, 342, 988
BseMI GCAATG 2 cut(s) 571, 781
BseRI GAGGAG 2 cut(s) 136, 179
BseXI GCAGC 4 cut(s) 327, 486, 853, 1137
BseYI CCCAGC 1 cut(s) 844
BsgI GTGCAG 2 cut(s) 1051, 1056
Bsh1236I CGCG 2 cut(s) 249, 420
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 4 cut(s) 306, 404, 749, 790
BsiEI CGRYCG 1 cut(s) 239
BsiHKCI CYCGRG 1 cut(s) 647
BsiSI CCGG 6 cut(s) 220, 235, 426, 581, 787, 942
BslI CCNNNNNNNGG 5 cut(s) 91, 219, 253, 342, 988
BsmAI GTCTC 1 cut(s) 650
BsnI GGCC 4 cut(s) 306, 404, 749, 790
BsoBI CYCGRG 1 cut(s) 647
Bsp1407I TGTACA 1 cut(s) 1110
Bsp143I GATC 3 cut(s) 240, 606, 994
Bsp68I TCGCGA 1 cut(s) 420
BspACI CCGC 7 cut(s) 216, 247, 259, 457, 460, 463, 1055
BspANI GGCC 4 cut(s) 306, 404, 749, 790
BspFNI CGCG 2 cut(s) 249, 420
BspHI TCATGA 2 cut(s) 1078, 1160
BspMAI CTGCAG 2 cut(s) 179, 1051
BspMI ACCTGC 3 cut(s) 107, 182, 1028
BspPI GGATC 2 cut(s) 235, 1002
BsrBI CCGCTC 1 cut(s) 261
BsrDI GCAATG 2 cut(s) 571, 781
BsrFI RCCGGY 1 cut(s) 786
BsrGI TGTACA 1 cut(s) 1110
BssAI RCCGGY 1 cut(s) 786
BssECI CCNNGG 1 cut(s) 942
BssMI GATC 3 cut(s) 240, 606, 994
BssNI GRCGYC 1 cut(s) 165
Bst4CI ACNGT 3 cut(s) 289, 809, 1118
BstACI GRCGYC 1 cut(s) 165
BstAUI TGTACA 1 cut(s) 1110
BstBAI YACGTR 2 cut(s) 594, 804
BstC8I GCNNGC 4 cut(s) 395, 541, 578, 788
BstEII GGTNACC 1 cut(s) 94
BstF5I GGATG 6 cut(s) 192, 511, 541, 589, 876, 1006
BstFNI CGCG 2 cut(s) 249, 420
BstHHI GCGC 2 cut(s) 414, 683
BstKTI GATC 3 cut(s) 243, 609, 997
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 3 cut(s) 240, 606, 994
BstMCI CGRYCG 1 cut(s) 239
BstMWI GCNNNNNNNGC 5 cut(s) 273, 312, 426, 577, 947
BstNSI RCATGY 4 cut(s) 298, 457, 499, 973
BstPI GGTNACC 1 cut(s) 94
BstSCI CCNGG 2 cut(s) 218, 941
BstSFI CTRYAG 2 cut(s) 175, 1047
BstSNI TACGTA 2 cut(s) 594, 804
BstUI CGCG 2 cut(s) 249, 420
BstV1I GCAGC 4 cut(s) 327, 486, 853, 1137
BstV2I GAAGAC 1 cut(s) 330
BsuRI GGCC 4 cut(s) 306, 404, 749, 790
BtgZI GCGATG 1 cut(s) 651
BtsCI GGATG 6 cut(s) 192, 511, 541, 589, 876, 1006
BtsI GCAGTG 1 cut(s) 1039
BtsIMutI CAGTG 2 cut(s) 814, 1039
BtuMI TCGCGA 1 cut(s) 420
BveI ACCTGC 3 cut(s) 107, 182, 1028
Cac8I GCNNGC 4 cut(s) 395, 541, 578, 788
CciI TCATGA 2 cut(s) 1078, 1160
CfoI GCGC 2 cut(s) 414, 683
Cfr10I RCCGGY 1 cut(s) 786
Cfr13I GGNCC 1 cut(s) 256
CseI GACGC 1 cut(s) 173
Csp6I GTAC 5 cut(s) 595, 773, 805, 883, 1111
CviQI GTAC 5 cut(s) 595, 773, 805, 883, 1111
DpnI GATC 3 cut(s) 242, 608, 996
DpnII GATC 3 cut(s) 240, 606, 994
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
Eco105I TACGTA 2 cut(s) 594, 804
Eco147I AGGCCT 1 cut(s) 404
Eco47I GGWCC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 383
Eco88I CYCGRG 1 cut(s) 647
Eco91I GGTNACC 1 cut(s) 94
EcoO65I GGTNACC 1 cut(s) 94
EcoT22I ATGCAT 2 cut(s) 907, 1006
FauI CCCGC 2 cut(s) 223, 456
FbaI TGATCA 1 cut(s) 606
Fnu4HI GCNGC 6 cut(s) 316, 458, 461, 500, 842, 1126
FokI GGATG 6 cut(s) 179, 518, 548, 596, 883, 1013
Fsp4HI GCNGC 6 cut(s) 316, 458, 461, 500, 842, 1126
FspBI CTAG 1 cut(s) 230
GlaI GCGC 2 cut(s) 413, 682
GluI GCNGC 6 cut(s) 316, 458, 461, 500, 842, 1126
GsaI CCCAGC 1 cut(s) 848
GsuI CTGGAG 1 cut(s) 247
HaeIII GGCC 4 cut(s) 306, 404, 749, 790
HapII CCGG 6 cut(s) 220, 235, 426, 581, 787, 942
HgaI GACGC 1 cut(s) 173
HhaI GCGC 2 cut(s) 414, 683
Hin1I GRCGYC 1 cut(s) 165
Hin6I GCGC 2 cut(s) 412, 681
HinP1I GCGC 2 cut(s) 412, 681
HincII GTYRAC 3 cut(s) 292, 742, 1060
HindII GTYRAC 3 cut(s) 292, 742, 1060
HinfI GANTC 5 cut(s) 183, 361, 422, 712, 820
HpaII CCGG 6 cut(s) 220, 235, 426, 581, 787, 942
HphI GGTGA 5 cut(s) 31, 52, 163, 361, 679
Hpy166II GTNNAC 7 cut(s) 207, 292, 591, 597, 670, 742, 1060
Hpy188I TCNGA 9 cut(s) 10, 22, 31, 70, 79, 128, 611, 687, 729
Hpy188III TCNNGA 8 cut(s) 85, 419, 437, 659, 763, 998, 1079, 1161
Hpy8I GTNNAC 7 cut(s) 207, 292, 591, 597, 670, 742, 1060
Hpy99I CGWCG 2 cut(s) 167, 524
HpyAV CCTTC 4 cut(s) 25, 178, 415, 830
HpyCH4III ACNGT 3 cut(s) 289, 809, 1118
HpyCH4IV ACGT 2 cut(s) 593, 803
HpyCH4V TGCA 9 cut(s) 177, 539, 833, 905, 966, 1004, 1032, 1037, 1049
HpyF10VI GCNNNNNNNGC 5 cut(s) 273, 312, 426, 577, 947
HpySE526I ACGT 2 cut(s) 593, 803
Hsp92I GRCGYC 1 cut(s) 165
HspAI GCGC 2 cut(s) 412, 681
KroI GCCGGC 1 cut(s) 786
KroNI GCCGGC 1 cut(s) 788
Ksp22I TGATCA 1 cut(s) 606
Kzo9I GATC 3 cut(s) 240, 606, 994
LmnI GCTCC 4 cut(s) 266, 688, 729, 975
Lsp1109I GCAGC 4 cut(s) 327, 486, 853, 1137
LweI GCATC 4 cut(s) 496, 526, 684, 991
MaeI CTAG 1 cut(s) 230
MaeII ACGT 2 cut(s) 593, 803
MaeIII GTNAC 2 cut(s) 94, 467
MalI GATC 3 cut(s) 242, 608, 996
MbiI CCGCTC 1 cut(s) 261
MboI GATC 3 cut(s) 240, 606, 994
MboII GAAGA 7 cut(s) 115, 335, 364, 574, 577, 889, 1149
MluCI AATT 5 cut(s) 119, 151, 623, 797, 929
MmeI TCCRAC 1 cut(s) 999
Mph1103I ATGCAT 2 cut(s) 907, 1006
MroNI GCCGGC 1 cut(s) 786
MslI CAYNNNNRTG 4 cut(s) 452, 602, 755, 1159
MspA1I CMGCKG 1 cut(s) 844
MspI CCGG 6 cut(s) 220, 235, 426, 581, 787, 942
MspR9I CCNGG 2 cut(s) 220, 943
MvnI CGCG 2 cut(s) 249, 420
MwoI GCNNNNNNNGC 5 cut(s) 273, 312, 426, 577, 947
NaeI GCCGGC 1 cut(s) 788
NciI CCSGG 2 cut(s) 220, 943
NdeII GATC 3 cut(s) 240, 606, 994
NgoMIV GCCGGC 1 cut(s) 786
NruI TCGCGA 1 cut(s) 420
NsiI ATGCAT 2 cut(s) 907, 1006
NspI RCATGY 4 cut(s) 298, 457, 499, 973
PagI TCATGA 2 cut(s) 1078, 1160
PaqCI CACCTGC 1 cut(s) 182
PceI AGGCCT 1 cut(s) 404
PciI ACATGT 1 cut(s) 969
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 788
PfeI GAWTC 5 cut(s) 183, 361, 422, 712, 820
PflFI GACNNNGTC 2 cut(s) 230, 892
PflMI CCANNNNNTGG 1 cut(s) 988
PkrI GCNGC 6 cut(s) 317, 459, 462, 501, 843, 1127
Ppu21I YACGTR 2 cut(s) 594, 804
PscI ACATGT 1 cut(s) 969
PsiI TTATAA 1 cut(s) 702
PspEI GGTNACC 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 844
PspPI GGNCC 1 cut(s) 256
PstI CTGCAG 2 cut(s) 179, 1051
PsyI GACNNNGTC 2 cut(s) 230, 892
PvuII CAGCTG 1 cut(s) 844
RruI TCGCGA 1 cut(s) 420
RsaI GTAC 5 cut(s) 596, 774, 806, 884, 1112
RsaNI GTAC 5 cut(s) 595, 773, 805, 883, 1111
RseI CAYNNNNRTG 4 cut(s) 452, 602, 755, 1159
SatI GCNGC 6 cut(s) 316, 458, 461, 500, 842, 1126
Sau3AI GATC 3 cut(s) 240, 606, 994
Sau96I GGNCC 1 cut(s) 256
ScrFI CCNGG 2 cut(s) 220, 943
SfaNI GCATC 4 cut(s) 496, 526, 684, 991
SfcI CTRYAG 2 cut(s) 175, 1047
SinI GGWCC 1 cut(s) 256
SmiMI CAYNNNNRTG 4 cut(s) 452, 602, 755, 1159
SnaBI TACGTA 2 cut(s) 594, 804
Sse9I AATT 5 cut(s) 119, 151, 623, 797, 929
SseBI AGGCCT 1 cut(s) 404
SsiI CCGC 7 cut(s) 216, 247, 259, 457, 460, 463, 1055
SspMI CTAG 1 cut(s) 230
StuI AGGCCT 1 cut(s) 404
StyD4I CCNGG 2 cut(s) 218, 941
TaaI ACNGT 3 cut(s) 289, 809, 1118
TaiI ACGT 2 cut(s) 596, 806
TaqI TCGA 7 cut(s) 181, 225, 239, 438, 450, 764, 875
TasI AATT 5 cut(s) 119, 151, 623, 797, 929
TatI WGTACW 2 cut(s) 882, 1110
TauI GCSGC 2 cut(s) 460, 463
TfiI GAWTC 5 cut(s) 183, 361, 422, 712, 820
TscAI CASTG 2 cut(s) 814, 1039
TseI GCWGC 4 cut(s) 315, 499, 841, 1125
TspDTI ATGAA 5 cut(s) 386, 575, 810, 1067, 1149
TspRI CASTG 2 cut(s) 814, 1039
Tth111I GACNNNGTC 2 cut(s) 230, 892
Van91I CCANNNNNTGG 1 cut(s) 988
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 2 cut(s) 119, 797
XceI RCATGY 4 cut(s) 298, 457, 499, 973
XspI CTAG 1 cut(s) 230
Zsp2I ATGCAT 2 cut(s) 907, 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.