Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0013399

Physical Location & Seq

Genomic Coordinates Forward (+)

27348102 .. 27350233

Gene Structure 2132 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00104260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1959 bp

ATGAGACACCGCCGCCACCTGAGACTGGTTCTGGCAATCCCCACGCGCCGCCACTACGCCACAAAATACACCGCCAAAGTAACCTCAACCTCCCCAACCGGCCGCACCCTCTCCGTCGAGGTGACCCCACCTCCTCCCCTCCCCACCGACATCCGCGGCTACACTCTCCCCCGCCGCGACCTCGTCTGCAAAGCCACCCAAATCATCCTCCACCAATCCCGCTCCGCCACGTCGGACCCCTTCCTCGACCTCTCCGACTACCTCTCCTCCCTCTCCCTCTCCCTCACCCCCGCCGAAGCCTCCGAGATCCTCAAGTCCCTAAACGACCCCGACCTCGCCTTAAAATTCTTCCGATTCTGCCCCTCCCTTTCCCCCAAATTCCAACACGACGCCTTCACCTGCAACCGCCTCCTCCTCATCCTCTCCAAGTCCGCCGCCCCCGCCCGCTTCGACCTCGCCCGCTCGATCGTCTCCGACATGCACCGCTCCAACACACGCGGCAACATCTCCACCGTCAATCTCCTCATCGGCCTCTTCGGCAGCACCGACGACCTGGAAACCACCATGGGCCTGGTGAAGAAATGGAGCCTCAACATGACCTCCTACACATACAAATGTCTAGTCCAGGCCTACTTGCGATCCTACAATTCCACCAAGGCCTTCCAGTCCTACATGGAAATGCGTCGCCATGGTCACAAATTGGACATTTACTCTTACAATATGCTGCTGCATGCGCTTGCCAAAGACGAAAAGGTTGAGCAGGCACACAAGGTGTTTGAAGATATGAAGAGGAAGGATTGCGAGCCGGATGAGTTTACATACACAATTATGATCAGAATGAGTGGGAAATTGGGTAAAGGGGATGAGTGTCTGGCGTTTTTTCGGGAATTGTTGAACAAAGGTTGCAATGTGAATTTGATTGTTTATAATACCGTCATTCAGGCTCTTGCTAGGAGCAACATGGTTGATAAGGCTGTCCTTGTGTTTGCGAAAATGGTGGAAGCTGATTGTAGGCCGAATGAGTTTACTTACAGTGTGATTTTGAATCTTTTGGTTGCGCAAGGGCAGCTTGGTAGGTTAGATGAGGTGGTGGAGATGTCGAAGAGATATGTGACTAAGTCTATATACGCGTATCTTGTTAAGACGTTGAGCAAATTGGGTCATGATGATGAAGCCCACCGGCTGTTCTGCAACATGTGGAAATTCCATGACAAGGGGGATCGGGATGCGTACATGTCGATGTTAGAGAGTTTGTGTTCTGCAGGTAAAACCGCGGAGGCCGTTGATATGTTGAGTAACATTCATGAGAAAGGGGTATCCACTGACACTATGATGTATAACACAGTGTTGGCCGCGCTTGGGAGGTTGAAGCAAATATCAAATCTTCATGAAGTGTATGAAAAGATGAAAAAGGATGGCCCTGAACCGGATATATTTACCTACAACATTATGATTTCCAGCTTTGGTAAGGCTGGGAAGGTTGATGAGGCGATGACAATATTCGAACAGCTTGAGAACAGTGATTGCAAACCTGATATTGTATCTTACAATTCATTGATCAATTGCCTTGGGAAGAATGGTGATGTGGATGAAGCCCACATGAGGTTCAAGGAGATGCAGGAGAAAGGGTTCAGCCCTGATGTTGTCACCTACAGCACTCTAATCGAGTGCTTTGGCAAGACAGATAGGGTTGAAATGGCGTGCAGGTTGTTTGATGAGATGCTTGCTCAAGGCTGCTGTCCCAACATTGTCACCTACAACATCTTGCTTGATTGTCTTGAGAGGTGTGGGAGGACCGCCGAGGCGGTGGATTTGTATGCAAAACTAAAGCAGCAGGGTTTGACGCCGGATTGGATTACATATTCAGTGCTTGAACGACTGCAAAGTGGTTCTCATAGGAAATTCAGAGTTCGTAGGCAGAGTCCAATCACAGGTTGGGTTGTTAGCCCTATAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0033993 GO:0034641 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050896 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:1901360 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

652

Amino Acids

74.06

Weight (kDa)

8.62

Isoelectric Point (pI)

42.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	201 - 248	4.8e-09	PPR repeat family
PPR_long	PF17177	208 - 373	1e-18	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	226 - 283	2.9e-11	Pentatricopeptide repeat domain
TPR_24	PF23276	228 - 331	4.2e-08	Fungal tetratrico peptide repeats
PPR_1	PF12854	231 - 263	5.3e-06	PPR repeat
PPR_2	PF13041	235 - 280	4.7e-17	PPR repeat family
PPR	PF01535	237 - 267	6.7e-06	PPR repeat
PPR_3	PF13812	294 - 352	4.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	300 - 333	1.8e-06	PPR repeat
PPR_2	PF13041	305 - 351	2e-12	PPR repeat family
TPR_24	PF23276	411 - 503	4.5e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	431 - 490	1.4e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	442 - 489	2.8e-11	PPR repeat family
PPR_long	PF17177	461 - 626	1.8e-12	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	473 - 504	1.2e-10	PPR repeat
PPR_2	PF13041	476 - 523	3.6e-14	PPR repeat family
PPR	PF01535	479 - 509	1.4e-07	PPR repeat
TPR_24	PF23276	490 - 608	5.6e-09	Fungal tetratrico peptide repeats
PPR_1	PF12854	508 - 539	7.1e-10	PPR repeat
PPR_2	PF13041	511 - 559	7.3e-18	PPR repeat family
PPR	PF01535	514 - 544	2.6e-09	PPR repeat
PPR_3	PF13812	536 - 591	1.2e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	542 - 574	9.2e-10	PPR repeat
PPR_2	PF13041	546 - 593	1.3e-16	PPR repeat family
PPR	PF01535	549 - 579	7.7e-07	PPR repeat
PPR_3	PF13812	569 - 624	6.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	578 - 605	5e-07	PPR repeat
PPR_2	PF13041	581 - 624	4.6e-12	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013399)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51965
fragaria_vesca	FvH4_6g32590
malus_domestica	MD09G1200200.v1.1
prunus_persica	Prupe.3G056800_v2.0.a1
pyrus_communis	pycom09g11780 pycom15g36760
rosa_chinensis	RchiOBHm_Chr2g0141041
rosa_laevigata	RLG00000019910
rosa_multiflora	Rmu_sc0004769.1_g000016 Rmu_sc0012897.1_g000006
rosa_roxburghii	Rroxscaffold_2G00104260
rosa_rugosa	Rorug02G0366300
rosa_samantha	Rh2AG417700 Rh2BG427600 Rh2CG404000 Rh2DG437100
rosa_wichuraiana	Rw2G034190 Rw2G034250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	927
AarI	CACCTGC	1 cut(s)	407
Acc16I	TGCGCA	1 cut(s)	1059
Acc36I	ACCTGC	3 cut(s)	407, 1253, 1695
AccBSI	CCGCTC	3 cut(s)	222, 462, 486
AccII	CGCG	7 cut(s)	46, 156, 177, 498, 1130, 1274, 1355
AclWI	GGATC	3 cut(s)	301, 633, 1227
AcoI	YGGCCR	2 cut(s)	100, 1350
AcsI	RAATTY	5 cut(s)	344, 377, 913, 1202, 1901
AcyI	GRCGYC	2 cut(s)	390, 1844
AdeI	CACNNNGTG	1 cut(s)	772
AfaI	GTAC	1 cut(s)	1232
AfiI	CCNNNNNNNGG	6 cut(s)	25, 1213, 1359, 1426, 1602, 1931
AflIII	ACRYGT	3 cut(s)	1128, 1194, 1233
AgsI	TTSAA	7 cut(s)	779, 895, 1045, 1369, 1609, 1694, 1874
AjiI	CACGTC	1 cut(s)	231
AjnI	CCWGG	3 cut(s)	552, 570, 624
AloI	GAACNNNNNNTCC	2 cut(s)	1613, 1645
AluBI	AGCT	4 cut(s)	1004, 1069, 1461, 1510
AluI	AGCT	4 cut(s)	1004, 1069, 1461, 1510
Alw26I	GTCTC	2 cut(s)	16, 475
AlwI	GGATC	3 cut(s)	301, 633, 1227
AoxI	GGCC	9 cut(s)	100, 529, 568, 627, 657, 1013, 1278, 1350, 1417
ApeKI	GCWGC	6 cut(s)	540, 724, 727, 1066, 1734, 1831
ApoI	RAATTY	5 cut(s)	344, 377, 913, 1202, 1901
ArsI	GACNNNNNNTTYG	2 cut(s)	606, 638
Asp700I	GAANNNNTTC	1 cut(s)	1628
AspLEI	GCGC	4 cut(s)	48, 736, 1060, 1357
AspS9I	GGNCC	4 cut(s)	235, 568, 1418, 1794
AsuHPI	GGTGA	7 cut(s)	133, 277, 388, 586, 1592, 1639, 1744
AsuII	TTCGAA	1 cut(s)	1503
AvaII	GGWCC	2 cut(s)	235, 1794
BbvI	GCAGC	6 cut(s)	552, 711, 714, 1078, 1721, 1843
BccI	CCATC	1 cut(s)	1409
BceAI	ACGGC	1 cut(s)	1265
BcgI	CGANNNNNNTGC	2 cut(s)	1645, 1679
BciT130I	CCWGG	3 cut(s)	554, 572, 626
BciVI	GTATCC	1 cut(s)	1327
BclI	TGATCA	2 cut(s)	831, 1557
BcoDI	GTCTC	2 cut(s)	16, 475
BfaI	CTAG	2 cut(s)	620, 951
BfmI	CTRYAG	2 cut(s)	1260, 1651
BfuAI	ACCTGC	3 cut(s)	407, 1253, 1695
BfuI	GTATCC	1 cut(s)	1327
BglI	GCCNNNNNGGC	1 cut(s)	537
Bme1390I	CCNGG	3 cut(s)	554, 572, 626
Bme18I	GGWCC	2 cut(s)	235, 1794
BmgBI	CACGTC	1 cut(s)	231
BmgT120I	GGNCC	4 cut(s)	235, 568, 1418, 1794
BmiI	GGNNCC	2 cut(s)	237, 587
BmrFI	CCNGG	3 cut(s)	554, 572, 626
BmsI	GCATC	3 cut(s)	1216, 1605, 1710
Bpu14I	TTCGAA	1 cut(s)	1503
BpuEI	CTTGAG	4 cut(s)	296, 1532, 1713, 1799
BsaHI	GRCGYC	2 cut(s)	390, 1844
BsaJI	CCNNGG	7 cut(s)	154, 564, 654, 688, 1272, 1567, 1800
BsaWI	WCCGGW	1 cut(s)	1426
Bsc4I	CCNNNNNNNGG	6 cut(s)	25, 1213, 1359, 1426, 1602, 1931
Bse118I	RCCGGY	2 cut(s)	98, 1179
Bse1I	ACTGG	2 cut(s)	30, 664
Bse3DI	GCAATG	1 cut(s)	913
BseBI	CCWGG	3 cut(s)	554, 572, 626
BseDI	CCNNGG	7 cut(s)	154, 564, 654, 688, 1272, 1567, 1800
BseGI	GGATG	8 cut(s)	150, 204, 417, 814, 868, 1231, 1420, 1594
BseLI	CCNNNNNNNGG	6 cut(s)	25, 1213, 1359, 1426, 1602, 1931
BseMI	GCAATG	1 cut(s)	913
BseMII	CTCAG	1 cut(s)	11
BseNI	ACTGG	2 cut(s)	30, 664
BseRI	GAGGAG	5 cut(s)	123, 256, 401, 404, 512
BseX3I	CGGCCG	1 cut(s)	100
BseXI	GCAGC	6 cut(s)	552, 711, 714, 1078, 1721, 1843
BseYI	CCCAGC	1 cut(s)	1472
BsgI	GTGCAG	1 cut(s)	1723
Bsh1236I	CGCG	7 cut(s)	46, 156, 177, 498, 1130, 1274, 1355
Bsh1285I	CGRYCG	2 cut(s)	103, 468
BshFI	GGCC	9 cut(s)	102, 531, 570, 629, 659, 1015, 1280, 1352, 1419
BsiEI	CGRYCG	2 cut(s)	103, 468
BsiSI	CCGG	5 cut(s)	99, 806, 1180, 1427, 1847
BslFI	GGGAC	2 cut(s)	301, 1725
BslI	CCNNNNNNNGG	6 cut(s)	25, 1213, 1359, 1426, 1602, 1931
BsmAI	GTCTC	2 cut(s)	16, 475
BsmBI	CGTCTC	1 cut(s)	475
BsmFI	GGGAC	2 cut(s)	301, 1725
BsnI	GGCC	9 cut(s)	102, 531, 570, 629, 659, 1015, 1280, 1352, 1419
Bsp119I	TTCGAA	1 cut(s)	1503
Bsp143I	GATC	6 cut(s)	306, 465, 638, 831, 1219, 1557
Bsp19I	CCATGG	2 cut(s)	564, 688
BspANI	GGCC	9 cut(s)	102, 531, 570, 629, 659, 1015, 1280, 1352, 1419
BspCNI	CTCAG	1 cut(s)	12
BspFNI	CGCG	7 cut(s)	46, 156, 177, 498, 1130, 1274, 1355
BspHI	TCATGA	3 cut(s)	1162, 1303, 1387
BspLI	GGNNCC	2 cut(s)	237, 587
BspMAI	CTGCAG	1 cut(s)	1264
BspMI	ACCTGC	3 cut(s)	407, 1253, 1695
BspPI	GGATC	3 cut(s)	301, 633, 1227
BspT104I	TTCGAA	1 cut(s)	1503
BsrBI	CCGCTC	3 cut(s)	222, 462, 486
BsrDI	GCAATG	1 cut(s)	913
BsrFI	RCCGGY	2 cut(s)	98, 1179
BsrI	ACTGG	2 cut(s)	30, 664
BssAI	RCCGGY	2 cut(s)	98, 1179
BssECI	CCNNGG	7 cut(s)	154, 564, 654, 688, 1272, 1567, 1800
BssMI	GATC	6 cut(s)	306, 465, 638, 831, 1219, 1557
BssNI	GRCGYC	2 cut(s)	390, 1844
BssT1I	CCWWGG	4 cut(s)	564, 654, 688, 1567
Bst2UI	CCWGG	3 cut(s)	554, 572, 626
Bst4CI	ACNGT	5 cut(s)	514, 934, 1034, 1345, 1520
Bst6I	CTCTTC	3 cut(s)	539, 782, 1097
BstACI	GRCGYC	2 cut(s)	390, 1844
BstBI	TTCGAA	1 cut(s)	1503
BstC8I	GCNNGC	8 cut(s)	445, 460, 732, 738, 762, 803, 1702, 1725
BstDEI	CTNAG	2 cut(s)	20, 1116
BstDSI	CCRYGG	4 cut(s)	154, 564, 688, 1272
BstEII	GGTNACC	1 cut(s)	121
BstF5I	GGATG	8 cut(s)	150, 204, 417, 814, 868, 1231, 1420, 1594
BstFNI	CGCG	7 cut(s)	46, 156, 177, 498, 1130, 1274, 1355
BstHHI	GCGC	4 cut(s)	48, 736, 1060, 1357
BstKTI	GATC	6 cut(s)	309, 468, 641, 834, 1222, 1560
BstMAI	GTCTC	2 cut(s)	16, 475
BstMBI	GATC	6 cut(s)	306, 465, 638, 831, 1219, 1557
BstMCI	CGRYCG	2 cut(s)	103, 468
BstMWI	GCNNNNNNNGC	4 cut(s)	440, 537, 733, 1066
BstNI	CCWGG	3 cut(s)	554, 572, 626
BstNSI	RCATGY	4 cut(s)	481, 734, 1198, 1237
BstPI	GGTNACC	1 cut(s)	121
BstSCI	CCNGG	3 cut(s)	552, 570, 624
BstSFI	CTRYAG	2 cut(s)	1260, 1651
BstUI	CGCG	7 cut(s)	46, 156, 177, 498, 1130, 1274, 1355
BstV1I	GCAGC	6 cut(s)	552, 711, 714, 1078, 1721, 1843
BstX2I	RGATCY	1 cut(s)	306
BstXI	CCANNNNNNTGG	1 cut(s)	571
BstYI	RGATCY	1 cut(s)	306
BstZI	CGGCCG	1 cut(s)	100
BsuI	GTATCC	1 cut(s)	1327
BsuRI	GGCC	9 cut(s)	102, 531, 570, 629, 659, 1015, 1280, 1352, 1419
BtgI	CCRYGG	4 cut(s)	154, 564, 688, 1272
BtgZI	GCGATG	1 cut(s)	1505
BtrI	CACGTC	1 cut(s)	231
BtsCI	GGATG	8 cut(s)	150, 204, 417, 814, 868, 1231, 1420, 1594
BtsIMutI	CAGTG	5 cut(s)	1039, 1320, 1350, 1525, 1872
BveI	ACCTGC	3 cut(s)	407, 1253, 1695
Cac8I	GCNNGC	8 cut(s)	445, 460, 732, 738, 762, 803, 1702, 1725
CciI	TCATGA	3 cut(s)	1162, 1303, 1387
CfoI	GCGC	4 cut(s)	48, 736, 1060, 1357
Cfr10I	RCCGGY	2 cut(s)	98, 1179
Cfr13I	GGNCC	4 cut(s)	235, 568, 1418, 1794
Cfr42I	CCGCGG	2 cut(s)	157, 1275
CseI	GACGC	3 cut(s)	398, 671, 1852
Csp6I	GTAC	1 cut(s)	1231
CviQI	GTAC	1 cut(s)	1231
DdeI	CTNAG	2 cut(s)	20, 1116
DpnI	GATC	6 cut(s)	308, 467, 640, 833, 1221, 1559
DpnII	GATC	6 cut(s)	306, 465, 638, 831, 1219, 1557
DraIII	CACNNNGTG	1 cut(s)	772
EaeI	YGGCCR	2 cut(s)	100, 1350
EagI	CGGCCG	1 cut(s)	100
Eam1104I	CTCTTC	3 cut(s)	539, 782, 1097
EarI	CTCTTC	3 cut(s)	539, 782, 1097
EciI	GGCGGA	2 cut(s)	214, 421
EclXI	CGGCCG	1 cut(s)	100
Eco130I	CCWWGG	4 cut(s)	564, 654, 688, 1567
Eco147I	AGGCCT	2 cut(s)	629, 659
Eco47I	GGWCC	2 cut(s)	235, 1794
Eco52I	CGGCCG	1 cut(s)	100
Eco91I	GGTNACC	1 cut(s)	121
EcoO65I	GGTNACC	1 cut(s)	121
EcoRII	CCWGG	3 cut(s)	552, 570, 624
EcoT14I	CCWWGG	4 cut(s)	564, 654, 688, 1567
ErhI	CCWWGG	4 cut(s)	564, 654, 688, 1567
Esp3I	CGTCTC	1 cut(s)	475
FalI	AAGNNNNNCTT	2 cut(s)	1053, 1085
FaqI	GGGAC	2 cut(s)	301, 1725
FauI	CCCGC	6 cut(s)	179, 227, 298, 448, 452, 467
FbaI	TGATCA	2 cut(s)	831, 1557
FokI	GGATG	8 cut(s)	137, 191, 404, 821, 875, 1238, 1427, 1601
FspBI	CTAG	2 cut(s)	620, 951
FspI	TGCGCA	1 cut(s)	1059
GlaI	GCGC	4 cut(s)	47, 735, 1059, 1356
GsaI	CCCAGC	1 cut(s)	1476
HaeIII	GGCC	9 cut(s)	102, 531, 570, 629, 659, 1015, 1280, 1352, 1419
HapII	CCGG	5 cut(s)	99, 806, 1180, 1427, 1847
HgaI	GACGC	3 cut(s)	398, 671, 1852
HhaI	GCGC	4 cut(s)	48, 736, 1060, 1357
Hin1I	GRCGYC	2 cut(s)	390, 1844
Hin6I	GCGC	4 cut(s)	46, 734, 1058, 1355
HinP1I	GCGC	4 cut(s)	46, 734, 1058, 1355
HinfI	GANTC	3 cut(s)	354, 1045, 1921
HpaII	CCGG	5 cut(s)	99, 806, 1180, 1427, 1847
HphI	GGTGA	7 cut(s)	133, 277, 388, 586, 1592, 1639, 1744
Hpy166II	GTNNAC	2 cut(s)	816, 1026
Hpy188I	TCNGA	7 cut(s)	235, 256, 304, 353, 475, 836, 1907
Hpy188III	TCNNGA	6 cut(s)	884, 1163, 1223, 1304, 1388, 1778
Hpy8I	GTNNAC	2 cut(s)	816, 1026
Hpy99I	CGWCG	5 cut(s)	119, 235, 392, 551, 687
HpyAV	CCTTC	5 cut(s)	250, 403, 670, 787, 1471
HpyCH4III	ACNGT	5 cut(s)	514, 934, 1034, 1345, 1520
HpyCH4IV	ACGT	2 cut(s)	230, 1145
HpyF10VI	GCNNNNNNNGC	4 cut(s)	440, 537, 733, 1066
HpyF3I	CTNAG	2 cut(s)	20, 1116
HpySE526I	ACGT	2 cut(s)	230, 1145
Hsp92I	GRCGYC	2 cut(s)	390, 1844
HspAI	GCGC	4 cut(s)	46, 734, 1058, 1355
Ksp22I	TGATCA	2 cut(s)	831, 1557
KspI	CCGCGG	2 cut(s)	157, 1275
Kzo9I	GATC	6 cut(s)	306, 465, 638, 831, 1219, 1557
LmnI	GCTCC	4 cut(s)	227, 491, 585, 954
Lsp1109I	GCAGC	6 cut(s)	552, 711, 714, 1078, 1721, 1843
LweI	GCATC	3 cut(s)	1216, 1605, 1710
MaeI	CTAG	2 cut(s)	620, 951
MaeII	ACGT	2 cut(s)	230, 1145
MaeIII	GTNAC	7 cut(s)	79, 121, 692, 1111, 1295, 1645, 1750
MalI	GATC	6 cut(s)	308, 467, 640, 833, 1221, 1559
MbiI	CCGCTC	3 cut(s)	222, 462, 486
MboI	GATC	6 cut(s)	306, 465, 638, 831, 1219, 1557
MboII	GAAGA	8 cut(s)	340, 526, 589, 791, 799, 1114, 1376, 1585
MfeI	CAATTG	1 cut(s)	1561
MflI	RGATCY	1 cut(s)	306
MluI	ACGCGT	1 cut(s)	1128
MlyI	GAGTC	1 cut(s)	1930
MmeI	TCCRAC	5 cut(s)	213, 279, 406, 498, 513
MroXI	GAANNNNTTC	1 cut(s)	1628
MseI	TTAA	2 cut(s)	341, 1140
MslI	CAYNNNNRTG	6 cut(s)	613, 677, 827, 1167, 1238, 1331
MspA1I	CMGCKG	2 cut(s)	156, 1274
MspI	CCGG	5 cut(s)	99, 806, 1180, 1427, 1847
MspR9I	CCNGG	3 cut(s)	554, 572, 626
MunI	CAATTG	1 cut(s)	1561
MvaI	CCWGG	3 cut(s)	554, 572, 626
MvnI	CGCG	7 cut(s)	46, 156, 177, 498, 1130, 1274, 1355
MwoI	GCNNNNNNNGC	4 cut(s)	440, 537, 733, 1066
NcoI	CCATGG	2 cut(s)	564, 688
NdeII	GATC	6 cut(s)	306, 465, 638, 831, 1219, 1557
NlaIV	GGNNCC	2 cut(s)	237, 587
NmeAIII	GCCGAG	1 cut(s)	1825
NmuCI	GTSAC	5 cut(s)	121, 692, 1111, 1645, 1750
NsbI	TGCGCA	1 cut(s)	1059
NspI	RCATGY	4 cut(s)	481, 734, 1198, 1237
NspV	TTCGAA	1 cut(s)	1503
PaeI	GCATGC	1 cut(s)	734
PagI	TCATGA	3 cut(s)	1162, 1303, 1387
PaqCI	CACCTGC	1 cut(s)	407
PceI	AGGCCT	2 cut(s)	629, 659
PciI	ACATGT	2 cut(s)	1194, 1233
PcsI	WCGNNNNNNNCGW	2 cut(s)	252, 543
PdmI	GAANNNNTTC	1 cut(s)	1628
PfeI	GAWTC	2 cut(s)	354, 1045
PflFI	GACNNNGTC	2 cut(s)	182, 1117
Ple19I	CGATCG	1 cut(s)	468
PleI	GAGTC	1 cut(s)	1929
PpsI	GAGTC	1 cut(s)	1929
PscI	ACATGT	2 cut(s)	1194, 1233
PsiI	TTATAA	1 cut(s)	927
Psp6I	CCWGG	3 cut(s)	552, 570, 624
PspEI	GGTNACC	1 cut(s)	121
PspFI	CCCAGC	1 cut(s)	1472
PspGI	CCWGG	3 cut(s)	552, 570, 624
PspN4I	GGNNCC	2 cut(s)	237, 587
PspPI	GGNCC	4 cut(s)	235, 568, 1418, 1794
PstI	CTGCAG	1 cut(s)	1264
PsuI	RGATCY	1 cut(s)	306
PsyI	GACNNNGTC	2 cut(s)	182, 1117
PvuI	CGATCG	1 cut(s)	468
RsaI	GTAC	1 cut(s)	1232
RsaNI	GTAC	1 cut(s)	1231
RseI	CAYNNNNRTG	6 cut(s)	613, 677, 827, 1167, 1238, 1331
SacII	CCGCGG	2 cut(s)	157, 1275
SaqAI	TTAA	2 cut(s)	341, 1140
Sau3AI	GATC	6 cut(s)	306, 465, 638, 831, 1219, 1557
Sau96I	GGNCC	4 cut(s)	235, 568, 1418, 1794
SchI	GAGTC	1 cut(s)	1930
ScrFI	CCNGG	3 cut(s)	554, 572, 626
SfaNI	GCATC	3 cut(s)	1216, 1605, 1710
SfcI	CTRYAG	2 cut(s)	1260, 1651
Sfr303I	CCGCGG	2 cut(s)	157, 1275
SfuI	TTCGAA	1 cut(s)	1503
SgrBI	CCGCGG	2 cut(s)	157, 1275
SinI	GGWCC	2 cut(s)	235, 1794
SmiMI	CAYNNNNRTG	6 cut(s)	613, 677, 827, 1167, 1238, 1331
SmlI	CTYRAG	4 cut(s)	311, 1511, 1728, 1778
SmoI	CTYRAG	4 cut(s)	311, 1511, 1728, 1778
SphI	GCATGC	1 cut(s)	734
SseBI	AGGCCT	2 cut(s)	629, 659
SspI	AATATT	1 cut(s)	1500
SspMI	CTAG	2 cut(s)	620, 951
StuI	AGGCCT	2 cut(s)	629, 659
StyD4I	CCNGG	3 cut(s)	552, 570, 624
StyI	CCWWGG	4 cut(s)	564, 654, 688, 1567
TaaI	ACNGT	5 cut(s)	514, 934, 1034, 1345, 1520
TaiI	ACGT	2 cut(s)	233, 1148
TaqI	TCGA	8 cut(s)	117, 246, 450, 464, 1100, 1238, 1503, 1665
TauI	GCSGC	8 cut(s)	15, 51, 105, 159, 177, 437, 501, 1355
TfiI	GAWTC	2 cut(s)	354, 1045
Tru1I	TTAA	2 cut(s)	341, 1140
Tru9I	TTAA	2 cut(s)	341, 1140
TscAI	CASTG	5 cut(s)	1039, 1327, 1350, 1525, 1872
TseFI	GTSAC	5 cut(s)	121, 692, 1111, 1645, 1750
TseI	GCWGC	6 cut(s)	540, 724, 727, 1066, 1734, 1831
Tsp45I	GTSAC	5 cut(s)	121, 692, 1111, 1645, 1750
TspDTI	ATGAA	9 cut(s)	800, 1185, 1292, 1376, 1404, 1413, 1421, 1542, 1605
TspGWI	ACGGA	1 cut(s)	103
TspRI	CASTG	5 cut(s)	1039, 1327, 1350, 1525, 1872
Tth111I	GACNNNGTC	2 cut(s)	182, 1117
VpaK11BI	GGWCC	2 cut(s)	235, 1794
XapI	RAATTY	5 cut(s)	344, 377, 913, 1202, 1901
XceI	RCATGY	4 cut(s)	481, 734, 1198, 1237
XcmI	CCANNNNNNNNNTGG	2 cut(s)	568, 1932
XmnI	GAANNNNTTC	1 cut(s)	1628
XspI	CTAG	2 cut(s)	620, 951

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.