Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0010069

Physical Location & Seq

Genomic Coordinates Forward (+)

19668145 .. 19679541

Gene Structure 11397 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1206000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2670 bp

ATGGACTGCCATTCCCTTCTGGGCTCATCTTCTTCCCTATCCCCATACCCCAAACCAATACCCCTCACCAAAACCCAGCCAAAACCCCTCCCTCCCTCCACCGTCTCCTCCTCCTCCTCCTCCCACCTCTCACCCGCCGACGATGACGATGAAAACGATAAAACCCACAAACTCAATTTCGATTTCCTCAAGCTCTCTGTAACTCTCACCGTAATCTCCACCTCCCTCCCCCGAATCCCCACCGCCGTCGCCGCCGTCAAGGAGAAAAAACGCGCGCCCAAGAAGTCCACCCCCAAAAAGTCCGAAGCTTTATCTCCCCAAGAACTCGAGTCATGGTCCCAGGGACTCCCCGTCGTCTCCAATCGAATTCCGTACACCCAGCTGTTGGCGCTCAGTCAGGAAGGTAAGCTGAAGCATGTCATCAAGCCGCCGGGAATTGAATTGCAGAAAAGGGCAGAGCCTGTTCTGGTTGTTCTCGAAGATTCTCGTGTTCTACGCACCGTTTTGCCATCGGTGGATAGCGACAGGAGGTTCTGGGAGCAATGGGAGGAGTTGAAAATTGAGTCTTTCTGTGTTAATGCTTACACTCCGCCGCTTAAACGGCCGGAGGTGCCGACGCCGTACTTGGGCTTCGTGGCGAAATGGCCCCGGTTTCTGTCGTCGTTCTCCGGGAAGCCGAAGAAGGAGAGCAAGAGAGCTATGGAGTTGAGAAGGGCGAGGGAGGAGTTCAAGATGCAGAGGAAGGAGGAGTTGGATAGGATGAGGAAGGAGAGAGAGATGATTGACAAGGCAATGAAGGCTCAGAAGAAAGAGGAGGAGAGGAGAGTGAGGAGAGAAATGAGGAAGAAGAAGCACGACGATTCGTTGCGTGAGGCACGGAGGAATTACCAGGAGATGGCTAATGTATGGGCCAATTTGGCTCAGGACACCAATGTGGCTACTGCTCTTGGGTTGGTGTTCTTCTACATATTTTATAGGACTGTGGTGCTTAGTTATAGGAGGCAGAAGAAGGACTATGAGGATAGGTTGAAGATCGAAAAGGCGGAGGCAGAGGAGAGGAAGAAAATGAGGGAGTTGGAGAGGGAGATGGAGGGGATTGAGGGTGATGAGGAGGATGAGATTGAGCCAGGGAAGGGTGAGCAGAATCCTTATTTGAAAATGGCAATGCAGTTCATGAAGTCTGGTGCTCGTGTCCGACGAGCTCATAATAAGAGGCTGCCTCAGTATTTGGAGAGAGGCGTGGATGTCAAGTTTACAGATGTGGCAGGGCTCGGGAAGATTAGGCTTGAGCTTGAGGAGATTGTCAAGTTCTTCACTCATGGTGAAATGTATAGGAGAAGAGGAGTTAAAATACCAGGTGGGATACTGCTTTGTGGCCCTCCTGGAGTGGGTAAGACATTGCTTGCAAAAGCTGTTGCTGGTGAAGCAGGCGTGAACTTCTTTTCCATTTCGGCCTCTCAGTTCGTGGAGATATATGTTGGTGTTGGTGCTTCTCGTGTCCGAGCACTTTATCAGGAGGCAAGGGAAAATGCTCCATCAGTTGTCTTCATTGATGAGTTGGATGCCGTTGGAAGAGAGCGTGGTTTGATCAAGGGTTCTGGTGGACAGGAACGTGATGCTACTCTTAATCAGCTCCTTGTTTGCTTGGATGGGTTTGAAGGAAGAGGGGAAGTGATCACTATTGCTTCCACAAATAGACCAGATATTCTAGATCCAGCCCTTGTCAGACCTGGACGGTTTGACCGGAAGATATTTATCCCTAAGCCTGGTCTTATAGGTCGCATTGAAATTTTGAAGGTCCATGCACGCAAGAAGCCTATGGCTGAAGATGTCGACTATATGGCTATTGCTAGTATGACTGATGGAATGGTTGGTGCAGAGCTAGCTAACATAATTGAGGTTGCTGCAATCAACATGATGCGTGATGGAAGGACTGAGATTACTACTGATGACCTATTACAAGCTGCACAAATGGAAGAAAGAGGAATGCTAGATAGGAAGGAGAGAAGTTTAGATACATGGAAGCAAGTAGCTATTAATGAGGCTGCAATGGCTGTTGTTGCTGTGAACTTTCCTGATCTTAGAAATATTGAGTTTGTCACAATTGCTCCTAGAGCTGGTCGAGAATTGGGTTATGTTAGAATGAAAATGGATCCTATCAAGTTCAGAGAAGGAATGCTTACTCGGCAGTCGCTCCTCGATCATATCACTGTTCAACTGGCACCACGTGCAGCTGATGAACTTTGGTTTGGTAAGGATCAGTTAAGTACAATATGGGCTGAAACAGCAGACAATGCTAGGTCAGCAGCACGAACGTATGTTCTTGGTGGCCTTTCAGAGAAGCATCACGGGTTATCTAATTTCTGGGTTGCAGATCGACTCAATGACCTCGATGTGGAGGCATTGCAAATCGTCAACATGTGTTATGAACGTGCAAAGGAGATTTTGCAGAAAAACCGAACGCTCATGGATGCAGTGGTCGATGAACTTGTCGAGAAGAAGAGCTTAACCAAGCAAGAATTCTTTAGCTTAGTTGAGTTACATGGTACCCTCAAACCTATGCCGCCTAGCATACTCGACATCCGAGCTGCCAAACGGAAGCAGTTCCAAGAAATGATGATGAACCAGAAGGAAGCAGCTTTAGGTAGCAACTTACGAGCAAAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031967 GO:0031975 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

890

Amino Acids

100.91

Weight (kDa)

9.25

Isoelectric Point (pI)

44.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	455 - 587	1e-42	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	610 - 653	7.3e-11	AAA+ lid domain
Peptidase_M41	PF01434	675 - 842	6.3e-12	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010069)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16290
fragaria_vesca	FvH4_6g32150 FvH4_6g32150 FvH4_6g32150
malus_domestica	MD09G1206000.v1.1
prunus_persica	Prupe.3G061400_v2.0.a1 Prupe.3G065400_v2.0.a1
pyrus_communis	pycom09g12280
rosa_chinensis	RchiOBHm_Chr2g0140041
rosa_laevigata	RLG00000019844
rosa_multiflora	Rmu_co8464017.1_g000001 Rmu_co8471751.1_g000001 Rmu_sc0010982.1_g000001
rosa_roxburghii	Rroxscaffold_2G00105120
rosa_rugosa	Rorug02G0360600 Rorug02G0360700
rosa_samantha	Rh2AG411300 Rh2BG421500 Rh2CG397400 Rh2DG431000
rosa_wichuraiana	Rw2G033690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2546
AccB1I	GGYRCC	3 cut(s)	610, 2221, 2546
AccB7I	CCANNNNNTGG	2 cut(s)	385, 895
AccI	GTMKAC	1 cut(s)	1833
AccII	CGCG	2 cut(s)	273, 275
AciI	CCGC	8 cut(s)	135, 243, 252, 428, 590, 593, 1043, 2564
AclWI	GGATC	4 cut(s)	1706, 2147, 2160, 2265
AcoI	YGGCCR	1 cut(s)	602
AcsI	RAATTY	3 cut(s)	366, 1788, 2519
AcuI	CTGAAG	2 cut(s)	431, 1845
AcvI	CACGTG	1 cut(s)	2228
AcyI	GRCGYC	1 cut(s)	617
AdeI	CACNNNGTG	1 cut(s)	2228
AfaI	GTAC	4 cut(s)	374, 623, 2269, 2548
AfiI	CCNNNNNNNGG	8 cut(s)	385, 626, 895, 1133, 1388, 1766, 2117, 2395
AflIII	ACRYGT	1 cut(s)	2418
AgsI	TTSAA	9 cut(s)	440, 556, 730, 1030, 1156, 1658, 1787, 1795, 2216
AhdI	GACNNNNNGTC	1 cut(s)	350
AjnI	CCWGG	7 cut(s)	339, 888, 1126, 1354, 1381, 1729, 1765
AjuI	GAANNNNNNNTTGG	6 cut(s)	734, 766, 2232, 2264, 2504, 2536
AleI	CACNNNNGTG	1 cut(s)	932
AloI	GAACNNNNNNTCC	2 cut(s)	1427, 1459
Alw21I	GWGCWC	3 cut(s)	1189, 1204, 1507
Alw26I	GTCTC	2 cut(s)	109, 361
AlwI	GGATC	4 cut(s)	1706, 2147, 2160, 2265
AlwNI	CAGNNNCTG	1 cut(s)	461
Ama87I	CYCGRG	2 cut(s)	326, 1271
AoxI	GGCC	6 cut(s)	602, 644, 909, 1375, 1452, 2329
ApeKI	GCWGC	8 cut(s)	1216, 1904, 1964, 2045, 2231, 2306, 2588, 2636
ApoI	RAATTY	3 cut(s)	366, 1788, 2519
AseI	ATTAAT	1 cut(s)	2037
Asp700I	GAANNNNTTC	1 cut(s)	2603
Asp718I	GGTACC	1 cut(s)	2546
AspLEI	GCGC	3 cut(s)	275, 277, 391
AspS9I	GGNCC	5 cut(s)	336, 645, 909, 1376, 1798
AsuC2I	CCSGG	3 cut(s)	432, 649, 670
AsuHPI	GGTGA	7 cut(s)	58, 123, 199, 1115, 1148, 1334, 1433
AsuNHI	GCTAGC	1 cut(s)	1882
AvaI	CYCGRG	2 cut(s)	326, 1271
AvaII	GGWCC	2 cut(s)	336, 1798
BamHI	GGATCC	1 cut(s)	2152
BanI	GGYRCC	3 cut(s)	610, 2221, 2546
BanII	GRGCYC	3 cut(s)	26, 1204, 1272
BarI	GAAGNNNNNNTAC	4 cut(s)	614, 646, 1999, 2031
BauI	CACGAG	3 cut(s)	486, 1188, 1494
BbrPI	CACGTG	1 cut(s)	2228
BbsI	GAAGAC	1 cut(s)	1537
Bbv12I	GWGCWC	3 cut(s)	1189, 1204, 1507
BbvI	GCAGC	8 cut(s)	1203, 1891, 1951, 2032, 2243, 2318, 2575, 2648
BccI	CCATC	7 cut(s)	517, 889, 1081, 1543, 1643, 1856, 1919
BceAI	ACGGC	5 cut(s)	230, 239, 604, 617, 1550
BciT130I	CCWGG	7 cut(s)	341, 890, 1128, 1356, 1383, 1731, 1767
BciVI	GTATCC	1 cut(s)	1356
BclI	TGATCA	2 cut(s)	1587, 1674
BcnI	CCSGG	3 cut(s)	432, 649, 670
BcoDI	GTCTC	2 cut(s)	109, 361
BfaI	CTAG	7 cut(s)	1709, 1851, 1883, 1991, 2112, 2298, 2568
BfoI	RGCGCY	1 cut(s)	392
BfuI	GTATCC	1 cut(s)	1356
BglI	GCCNNNNNGGC	1 cut(s)	917
Bme18I	GGWCC	2 cut(s)	336, 1798
BmeRI	GACNNNNNGTC	1 cut(s)	350
BmeT110I	CYCGRG	2 cut(s)	326, 1271
BmgT120I	GGNCC	5 cut(s)	336, 645, 909, 1376, 1798
BmiI	GGNNCC	6 cut(s)	338, 612, 647, 2154, 2223, 2548
BmsI	GCATC	6 cut(s)	723, 1552, 1606, 1908, 2353, 2461
BmtI	GCTAGC	1 cut(s)	1886
BpiI	GAAGAC	1 cut(s)	1537
BplI	GAGNNNNNCTC	2 cut(s)	1204, 1236
BpmI	CTGGAG	1 cut(s)	1404
Bpu10I	CCTNAGC	2 cut(s)	921, 1761
BpuEI	CTTGAG	3 cut(s)	173, 1307, 1313
BpuMI	CCSGG	3 cut(s)	432, 649, 670
BsaAI	YACGTR	1 cut(s)	2228
BsaBI	GATNNNNATC	1 cut(s)	1754
BsaHI	GRCGYC	1 cut(s)	617
BsaJI	CCNNGG	4 cut(s)	339, 340, 647, 1127
BsaWI	WCCGGW	1 cut(s)	1743
BsaXI	ACNNNNNCTCC	2 cut(s)	2092, 2122
Bsc4I	CCNNNNNNNGG	8 cut(s)	385, 626, 895, 1133, 1388, 1766, 2117, 2395
Bse1I	ACTGG	1 cut(s)	2223
Bse3DI	GCAATG	6 cut(s)	548, 798, 1170, 1397, 2055, 2402
Bse8I	GATNNNNATC	1 cut(s)	1754
BseBI	CCWGG	7 cut(s)	341, 890, 1128, 1356, 1383, 1731, 1767
BseDI	CCNNGG	4 cut(s)	339, 340, 647, 1127
BseGI	GGATG	7 cut(s)	765, 1120, 1249, 1567, 1654, 2476, 2580
BseJI	GATNNNNATC	1 cut(s)	1754
BseLI	CCNNNNNNNGG	8 cut(s)	385, 626, 895, 1133, 1388, 1766, 2117, 2395
BseMI	GCAATG	6 cut(s)	548, 798, 1170, 1397, 2055, 2402
BseMII	CTCAG	6 cut(s)	406, 815, 935, 1235, 1472, 1926
BseNI	ACTGG	1 cut(s)	2223
BsePI	GCGCGC	1 cut(s)	273
BseX3I	CGGCCG	1 cut(s)	602
BseXI	GCAGC	8 cut(s)	1203, 1891, 1951, 2032, 2243, 2318, 2575, 2648
BseYI	CCCAGC	2 cut(s)	75, 378
BsgI	GTGCAG	3 cut(s)	1896, 1950, 2250
Bsh1236I	CGCG	2 cut(s)	273, 275
Bsh1285I	CGRYCG	1 cut(s)	605
BshFI	GGCC	6 cut(s)	604, 646, 911, 1377, 1454, 2331
BshNI	GGYRCC	3 cut(s)	610, 2221, 2546
BsiEI	CGRYCG	1 cut(s)	605
BsiHKAI	GWGCWC	3 cut(s)	1189, 1204, 1507
BsiHKCI	CYCGRG	2 cut(s)	326, 1271
BsiSI	CCGG	5 cut(s)	431, 605, 649, 669, 1744
BslFI	GGGAC	2 cut(s)	322, 357
BslI	CCNNNNNNNGG	8 cut(s)	385, 626, 895, 1133, 1388, 1766, 2117, 2395
BsmAI	GTCTC	2 cut(s)	109, 361
BsmBI	CGTCTC	2 cut(s)	109, 361
BsmFI	GGGAC	2 cut(s)	322, 357
BsmI	GAATGC	2 cut(s)	1992, 2181
BsnI	GGCC	6 cut(s)	604, 646, 911, 1377, 1454, 2331
BsoBI	CYCGRG	2 cut(s)	326, 1271
Bsp1286I	GDGCHC	5 cut(s)	26, 1189, 1204, 1272, 1507
Bsp143I	GATC	9 cut(s)	1032, 1587, 1674, 1711, 2077, 2152, 2200, 2257, 2374
BspACI	CCGC	8 cut(s)	135, 243, 252, 428, 590, 593, 1043, 2564
BspANI	GGCC	6 cut(s)	604, 646, 911, 1377, 1454, 2331
BspCNI	CTCAG	6 cut(s)	405, 814, 934, 1234, 1471, 1927
BspFNI	CGCG	2 cut(s)	273, 275
BspHI	TCATGA	1 cut(s)	1173
BspLI	GGNNCC	6 cut(s)	338, 612, 647, 2154, 2223, 2548
BspOI	GCTAGC	1 cut(s)	1886
BspPI	GGATC	4 cut(s)	1706, 2147, 2160, 2265
BspQI	GCTCTTC	1 cut(s)	2495
BspT107I	GGYRCC	3 cut(s)	610, 2221, 2546
BsrDI	GCAATG	6 cut(s)	548, 798, 1170, 1397, 2055, 2402
BsrI	ACTGG	1 cut(s)	2223
BssECI	CCNNGG	4 cut(s)	339, 340, 647, 1127
BssHII	GCGCGC	1 cut(s)	273
BssMI	GATC	9 cut(s)	1032, 1587, 1674, 1711, 2077, 2152, 2200, 2257, 2374
BssNI	GRCGYC	1 cut(s)	617
BssSI	CACGAG	3 cut(s)	486, 1188, 1494
Bst2BI	CACGAG	3 cut(s)	486, 1188, 1494
Bst2UI	CCWGG	7 cut(s)	341, 890, 1128, 1356, 1383, 1731, 1767
Bst4CI	ACNGT	6 cut(s)	103, 211, 502, 982, 1737, 2212
Bst6I	CTCTTC	4 cut(s)	1333, 1567, 1657, 2495
BstACI	GRCGYC	1 cut(s)	617
BstAPI	GCANNNNNTGC	2 cut(s)	2228, 2294
BstBAI	YACGTR	1 cut(s)	2228
BstC8I	GCNNGC	5 cut(s)	275, 1404, 1429, 1807, 1884
BstF5I	GGATG	7 cut(s)	765, 1120, 1249, 1567, 1654, 2476, 2580
BstFNI	CGCG	2 cut(s)	273, 275
BstH2I	RGCGCY	1 cut(s)	392
BstHHI	GCGC	3 cut(s)	275, 277, 391
BstKTI	GATC	9 cut(s)	1035, 1590, 1677, 1714, 2080, 2155, 2203, 2260, 2377
BstMAI	GTCTC	2 cut(s)	109, 361
BstMBI	GATC	9 cut(s)	1032, 1587, 1674, 1711, 2077, 2152, 2200, 2257, 2374
BstMCI	CGRYCG	1 cut(s)	605
BstNI	CCWGG	7 cut(s)	341, 890, 1128, 1356, 1383, 1731, 1767
BstNSI	RCATGY	2 cut(s)	419, 2422
BstUI	CGCG	2 cut(s)	273, 275
BstV1I	GCAGC	8 cut(s)	1203, 1891, 1951, 2032, 2243, 2318, 2575, 2648
BstV2I	GAAGAC	1 cut(s)	1537
BstX2I	RGATCY	2 cut(s)	1711, 2152
BstYI	RGATCY	2 cut(s)	1711, 2152
BstZI	CGGCCG	1 cut(s)	602
BsuI	GTATCC	1 cut(s)	1356
BsuRI	GGCC	6 cut(s)	604, 646, 911, 1377, 1454, 2331
BtsCI	GGATG	7 cut(s)	765, 1120, 1249, 1567, 1654, 2476, 2580
BtsI	GCAGTG	1 cut(s)	2481
BtsIMutI	CAGTG	2 cut(s)	2208, 2481
Cac8I	GCNNGC	5 cut(s)	275, 1404, 1429, 1807, 1884
CaiI	CAGNNNCTG	1 cut(s)	461
CciI	TCATGA	1 cut(s)	1173
CfoI	GCGC	3 cut(s)	275, 277, 391
Cfr13I	GGNCC	5 cut(s)	336, 645, 909, 1376, 1798
CseI	GACGC	1 cut(s)	625
CsiI	ACCWGGT	1 cut(s)	1354
Csp6I	GTAC	4 cut(s)	373, 622, 2268, 2547
CviQI	GTAC	4 cut(s)	373, 622, 2268, 2547
DpnI	GATC	9 cut(s)	1034, 1589, 1676, 1713, 2079, 2154, 2202, 2259, 2376
DpnII	GATC	9 cut(s)	1032, 1587, 1674, 1711, 2077, 2152, 2200, 2257, 2374
DraIII	CACNNNGTG	1 cut(s)	2228
DriI	GACNNNNNGTC	1 cut(s)	350
EaeI	YGGCCR	1 cut(s)	602
EagI	CGGCCG	1 cut(s)	602
Eam1104I	CTCTTC	4 cut(s)	1333, 1567, 1657, 2495
Eam1105I	GACNNNNNGTC	1 cut(s)	350
EarI	CTCTTC	4 cut(s)	1333, 1567, 1657, 2495
EciI	GGCGGA	2 cut(s)	579, 1058
Ecl136II	GAGCTC	1 cut(s)	1202
EclXI	CGGCCG	1 cut(s)	602
Eco24I	GRGCYC	3 cut(s)	26, 1204, 1272
Eco47I	GGWCC	2 cut(s)	336, 1798
Eco52I	CGGCCG	1 cut(s)	602
Eco53kI	GAGCTC	1 cut(s)	1202
Eco57I	CTGAAG	2 cut(s)	431, 1845
Eco72I	CACGTG	1 cut(s)	2228
Eco88I	CYCGRG	2 cut(s)	326, 1271
EcoICRI	GAGCTC	1 cut(s)	1202
EcoRI	GAATTC	2 cut(s)	366, 2519
EcoRII	CCWGG	7 cut(s)	339, 888, 1126, 1354, 1381, 1729, 1765
EcoT38I	GRGCYC	3 cut(s)	26, 1204, 1272
Esp3I	CGTCTC	2 cut(s)	109, 361
FalI	AAGNNNNNCTT	4 cut(s)	2163, 2195, 2489, 2521
FaqI	GGGAC	2 cut(s)	322, 357
FauI	CCCGC	1 cut(s)	142
FbaI	TGATCA	2 cut(s)	1587, 1674
FblI	GTMKAC	1 cut(s)	1833
FokI	GGATG	7 cut(s)	772, 1127, 1256, 1574, 1661, 2483, 2567
FriOI	GRGCYC	3 cut(s)	26, 1204, 1272
FspBI	CTAG	7 cut(s)	1709, 1851, 1883, 1991, 2112, 2298, 2568
GlaI	GCGC	3 cut(s)	274, 276, 390
GsaI	CCCAGC	2 cut(s)	79, 382
GsuI	CTGGAG	1 cut(s)	1404
HaeII	RGCGCY	1 cut(s)	392
HaeIII	GGCC	6 cut(s)	604, 646, 911, 1377, 1454, 2331
HapII	CCGG	5 cut(s)	431, 605, 649, 669, 1744
HgaI	GACGC	1 cut(s)	625
HhaI	GCGC	3 cut(s)	275, 277, 391
Hin1I	GRCGYC	1 cut(s)	617
Hin6I	GCGC	3 cut(s)	273, 275, 389
HinP1I	GCGC	3 cut(s)	273, 275, 389
HincII	GTYRAC	2 cut(s)	1834, 2416
HindII	GTYRAC	2 cut(s)	1834, 2416
HindIII	AAGCTT	1 cut(s)	306
HinfI	GANTC	8 cut(s)	234, 329, 345, 482, 563, 860, 1144, 2379
HpaII	CCGG	5 cut(s)	431, 605, 649, 669, 1744
HphI	GGTGA	7 cut(s)	58, 123, 199, 1115, 1148, 1334, 1433
Hpy166II	GTNNAC	8 cut(s)	288, 375, 1254, 1435, 1604, 1834, 2068, 2416
Hpy188I	TCNGA	8 cut(s)	304, 804, 1196, 1502, 1727, 2168, 2338, 2585
Hpy8I	GTNNAC	8 cut(s)	288, 375, 1254, 1435, 1604, 1834, 2068, 2416
Hpy99I	CGWCG	7 cut(s)	143, 251, 356, 619, 664, 860, 1200
HpyCH4III	ACNGT	6 cut(s)	103, 211, 502, 982, 1737, 2212
HpyCH4IV	ACGT	4 cut(s)	1612, 2227, 2315, 2431
HpySE526I	ACGT	4 cut(s)	1612, 2227, 2315, 2431
Hsp92I	GRCGYC	1 cut(s)	617
HspAI	GCGC	3 cut(s)	273, 275, 389
KpnI	GGTACC	1 cut(s)	2550
Ksp22I	TGATCA	2 cut(s)	1587, 1674
Kzo9I	GATC	9 cut(s)	1032, 1587, 1674, 1711, 2077, 2152, 2200, 2257, 2374
LguI	GCTCTTC	1 cut(s)	2495
LmnI	GCTCC	5 cut(s)	538, 1537, 1638, 2113, 2199
Lsp1109I	GCAGC	8 cut(s)	1203, 1891, 1951, 2032, 2243, 2318, 2575, 2648
LweI	GCATC	6 cut(s)	723, 1552, 1606, 1908, 2353, 2461
MabI	ACCWGGT	1 cut(s)	1354
MaeI	CTAG	7 cut(s)	1709, 1851, 1883, 1991, 2112, 2298, 2568
MaeII	ACGT	4 cut(s)	1612, 2227, 2315, 2431
MaeIII	GTNAC	3 cut(s)	199, 2098, 2538
MalI	GATC	9 cut(s)	1034, 1589, 1676, 1713, 2079, 2154, 2202, 2259, 2376
MboI	GATC	9 cut(s)	1032, 1587, 1674, 1711, 2077, 2152, 2200, 2257, 2374
MfeI	CAATTG	1 cut(s)	2103
MflI	RGATCY	2 cut(s)	1711, 2152
MhlI	GDGCHC	5 cut(s)	26, 1189, 1204, 1272, 1507
MlyI	GAGTC	4 cut(s)	338, 339, 572, 2373
MmeI	TCCRAC	5 cut(s)	732, 1056, 1219, 1539, 1549
MroXI	GAANNNNTTC	1 cut(s)	2603
MseI	TTAA	7 cut(s)	576, 597, 1347, 1626, 2037, 2264, 2507
MslI	CAYNNNNRTG	1 cut(s)	932
MspA1I	CMGCKG	2 cut(s)	382, 2234
MspI	CCGG	5 cut(s)	431, 605, 649, 669, 1744
MunI	CAATTG	1 cut(s)	2103
Mva1269I	GAATGC	2 cut(s)	1992, 2181
MvaI	CCWGG	7 cut(s)	341, 890, 1128, 1356, 1383, 1731, 1767
MvnI	CGCG	2 cut(s)	273, 275
NciI	CCSGG	3 cut(s)	432, 649, 670
NdeII	GATC	9 cut(s)	1032, 1587, 1674, 1711, 2077, 2152, 2200, 2257, 2374
NheI	GCTAGC	1 cut(s)	1882
NlaIV	GGNNCC	6 cut(s)	338, 612, 647, 2154, 2223, 2548
NmeAIII	GCCGAG	1 cut(s)	2164
NmuCI	GTSAC	1 cut(s)	2098
NspI	RCATGY	2 cut(s)	419, 2422
OliI	CACNNNNGTG	1 cut(s)	932
PaeR7I	CTCGAG	1 cut(s)	326
PagI	TCATGA	1 cut(s)	1173
PasI	CCCWGGG	1 cut(s)	340
PauI	GCGCGC	1 cut(s)	273
PciI	ACATGT	1 cut(s)	2418
PciSI	GCTCTTC	1 cut(s)	2495
PcsI	WCGNNNNNNNCGW	2 cut(s)	153, 1195
PctI	GAATGC	2 cut(s)	1992, 2181
PdmI	GAANNNNTTC	1 cut(s)	2603
PfeI	GAWTC	4 cut(s)	234, 482, 860, 1144
PflMI	CCANNNNNTGG	2 cut(s)	385, 895
PfoI	TCCNGGA	2 cut(s)	668, 1381
PleI	GAGTC	4 cut(s)	337, 339, 571, 2373
PmaCI	CACGTG	1 cut(s)	2228
PmlI	CACGTG	1 cut(s)	2228
PpsI	GAGTC	4 cut(s)	337, 339, 571, 2373
Ppu21I	YACGTR	1 cut(s)	2228
PscI	ACATGT	1 cut(s)	2418
PshBI	ATTAAT	1 cut(s)	2037
Psp124BI	GAGCTC	1 cut(s)	1204
Psp6I	CCWGG	7 cut(s)	339, 888, 1126, 1354, 1381, 1729, 1765
PspCI	CACGTG	1 cut(s)	2228
PspFI	CCCAGC	2 cut(s)	75, 378
PspGI	CCWGG	7 cut(s)	339, 888, 1126, 1354, 1381, 1729, 1765
PspN4I	GGNNCC	6 cut(s)	338, 612, 647, 2154, 2223, 2548
PspPI	GGNCC	5 cut(s)	336, 645, 909, 1376, 1798
PspXI	VCTCGAGB	1 cut(s)	326
PstNI	CAGNNNCTG	1 cut(s)	461
PsuI	RGATCY	2 cut(s)	1711, 2152
PteI	GCGCGC	1 cut(s)	273
PvuII	CAGCTG	2 cut(s)	382, 2234
RsaI	GTAC	4 cut(s)	374, 623, 2269, 2548
RsaNI	GTAC	4 cut(s)	373, 622, 2268, 2547
RseI	CAYNNNNRTG	1 cut(s)	932
SacI	GAGCTC	1 cut(s)	1204
SalI	GTCGAC	1 cut(s)	1832
SapI	GCTCTTC	1 cut(s)	2495
SaqAI	TTAA	7 cut(s)	576, 597, 1347, 1626, 2037, 2264, 2507
Sau3AI	GATC	9 cut(s)	1032, 1587, 1674, 1711, 2077, 2152, 2200, 2257, 2374
Sau96I	GGNCC	5 cut(s)	336, 645, 909, 1376, 1798
SchI	GAGTC	4 cut(s)	338, 339, 572, 2373
SduI	GDGCHC	5 cut(s)	26, 1189, 1204, 1272, 1507
SexAI	ACCWGGT	1 cut(s)	1354
SfaNI	GCATC	6 cut(s)	723, 1552, 1606, 1908, 2353, 2461
Sfr274I	CTCGAG	1 cut(s)	326
SinI	GGWCC	2 cut(s)	336, 1798
SlaI	CTCGAG	1 cut(s)	326
SmiMI	CAYNNNNRTG	1 cut(s)	932
SmlI	CTYRAG	4 cut(s)	188, 326, 1286, 1292
SmoI	CTYRAG	4 cut(s)	188, 326, 1286, 1292
SsiI	CCGC	8 cut(s)	135, 243, 252, 428, 590, 593, 1043, 2564
SspI	AATATT	1 cut(s)	2089
SspMI	CTAG	7 cut(s)	1709, 1851, 1883, 1991, 2112, 2298, 2568
SstI	GAGCTC	1 cut(s)	1204
TaaI	ACNGT	6 cut(s)	103, 211, 502, 982, 1737, 2212
TaiI	ACGT	4 cut(s)	1615, 2230, 2318, 2434
TatI	WGTACW	1 cut(s)	2267
TauI	GCSGC	4 cut(s)	254, 430, 595, 2566
TfiI	GAWTC	4 cut(s)	234, 482, 860, 1144
Tru1I	TTAA	7 cut(s)	576, 597, 1347, 1626, 2037, 2264, 2507
Tru9I	TTAA	7 cut(s)	576, 597, 1347, 1626, 2037, 2264, 2507
TscAI	CASTG	2 cut(s)	2215, 2481
TseFI	GTSAC	1 cut(s)	2098
TseI	GCWGC	8 cut(s)	1216, 1904, 1964, 2045, 2231, 2306, 2588, 2636
Tsp45I	GTSAC	1 cut(s)	2098
TspGWI	ACGGA	3 cut(s)	360, 892, 2611
TspRI	CASTG	2 cut(s)	2215, 2481
Van91I	CCANNNNNTGG	2 cut(s)	385, 895
VpaK11BI	GGWCC	2 cut(s)	336, 1798
VspI	ATTAAT	1 cut(s)	2037
XapI	RAATTY	3 cut(s)	366, 1788, 2519
XbaI	TCTAGA	1 cut(s)	1708
XceI	RCATGY	2 cut(s)	419, 2422
XhoI	CTCGAG	1 cut(s)	326
XmiI	GTMKAC	1 cut(s)	1833
XmnI	GAANNNNTTC	1 cut(s)	2603
XspI	CTAG	7 cut(s)	1709, 1851, 1883, 1991, 2112, 2298, 2568

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.