MD09G1240700.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015071
Physical Location & Seq
Forward (+)
30760751 .. 30761518
768 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1240700.v1.1.491

Sequence Viewer

Length: 768 bp
ATGTCTTCCTTGTCTACAATTCCGATGCGCCTCCTCAAGGATGACGAGACCGATCGGCCATGGTTCCATCACAGAAGCTCATACGACTTCAACAGCAAGATTATGCTAACAGCCATCATTTCATTATCCGTCGTTGTTTTGCTAGTAATCGTGCTTCATACCTACGCAAGGTGCGTCCTCCGACGCCAGGCCCTTCGCCGAGCGGCCTGGCATCATAATTTGGGGTCAACCGTGGCTCATGCCCACTCCGTTGAGTCTGAGCCACCCAAGAACGGACTCGAGCCCTCCGTCATCGCCGCCTTGCCCATATTCGTATTCACACGTGCGGGCGGTCGAGATGGCCGGGATGACAACGCAATAGAATGCGCTGTGTGTTTGAGTATGTTAGAGGATGAAGAAATGGCACGGTTGCTTCCAAATTGCAAGCATAGTTTCCATGCAGAGTGCATCGACAAGTGGCTGAGTTCCCACACTACCTGCCCCATTTGCCGGACAGAGGCGGAGCCACGTCTGCCGCTCGAGCCACGAGAGGGTCCCGCGGCCGGGACGCCCACGGCTCCACCATTGGAGCATGTGAATTCTGTGTTGTCATCATCATGTATGGAAGGGACATCAGAAGGGGCTCAATCCAGCTCAGTTGGTACTAAAGTAAATGCTCCAGTTTCGAGGTTGAGTTCTTTCAGAAGAATCCTTAGCATGGACAGATCTTCGAGACGGGTTCAATCTTGTGGTCAAGAAGATGGTATGGAAGACTTAGAGAGGCAGTGA

Protein Analysis

256

Amino Acids

28.18

Weight (kDa)

6.41

Isoelectric Point (pI)

65.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 121 - 164 7.7e-14 Ring finger domain
zf-C3HC4_2 PF13923 121 - 163 2.9e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 121 - 150 1.6e-09 RING-like zinc finger
zf-RING_5 PF14634 121 - 165 1.8e-06 zinc-RING finger domain
zf-rbx1 PF12678 122 - 164 4.2e-10 RING-H2 zinc finger domain
zf-C3HC4 PF00097 122 - 163 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 122 - 161 8.2e-06 RING-type zinc-finger
zf-ANAPC11 PF12861 136 - 166 4.2e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42350 AT2G42360
fragaria_vesca FvH4_6g27024
malus_domestica MD09G1240700.v1.1 MD17G1233600.v1.1
prunus_persica Prupe.3G125000_v2.0.a1
pyrus_communis pycom09g15940 pycom17g23670
rosa_chinensis RchiOBHm_Chr2g0128891
rosa_laevigata RLG00000019039
rosa_rugosa Rorug02G0282200
rosa_samantha Rh2AG334800 Rh2BG342800 Rh2CG321800 Rh2DG359400
rosa_wichuraiana Rw2G027080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 485
AccBSI CCGCTC 2 cut(s) 203, 517
AccI GTMKAC 1 cut(s) 14
AccII CGCG 1 cut(s) 539
AciI CCGC 8 cut(s) 203, 297, 326, 330, 500, 515, 537, 539
AcoI YGGCCR 3 cut(s) 56, 340, 540
AcsI RAATTY 1 cut(s) 577
AcvI CACGTG 1 cut(s) 323
AcyI GRCGYC 2 cut(s) 184, 548
AfaI GTAC 1 cut(s) 643
AflIII ACRYGT 1 cut(s) 320
AgsI TTSAA 2 cut(s) 91, 722
AjiI CACGTC 1 cut(s) 509
AjnI CCWGG 2 cut(s) 186, 206
AluBI AGCT 2 cut(s) 78, 633
AluI AGCT 2 cut(s) 78, 633
Alw26I GTCTC 2 cut(s) 41, 706
Ama87I CYCGRG 2 cut(s) 278, 518
AoxI GGCC 5 cut(s) 56, 189, 204, 340, 540
ApoI RAATTY 1 cut(s) 577
AspLEI GCGC 2 cut(s) 30, 368
AspS9I GGNCC 2 cut(s) 190, 533
AsuC2I CCSGG 2 cut(s) 344, 544
AvaI CYCGRG 2 cut(s) 278, 518
AvaII GGWCC 1 cut(s) 533
BaeI ACNNNNGTAYC 2 cut(s) 633, 666
BanII GRGCYC 2 cut(s) 285, 625
BarI GAAGNNNNNNTAC 2 cut(s) 138, 170
BauI CACGAG 1 cut(s) 525
BbrPI CACGTG 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 756
BccI CCATC 4 cut(s) 75, 122, 332, 734
BceAI ACGGC 1 cut(s) 570
BciT130I CCWGG 2 cut(s) 188, 208
BcnI CCSGG 2 cut(s) 344, 544
BcoDI GTCTC 2 cut(s) 41, 706
BfaI CTAG 1 cut(s) 143
BfuAI ACCTGC 1 cut(s) 485
BglII AGATCT 1 cut(s) 704
BisI GCNGC 4 cut(s) 204, 297, 515, 540
BlsI GCNGC 4 cut(s) 205, 298, 516, 541
Bme1390I CCNGG 4 cut(s) 188, 208, 344, 544
Bme18I GGWCC 1 cut(s) 533
BmeT110I CYCGRG 2 cut(s) 278, 518
BmgBI CACGTC 1 cut(s) 509
BmgT120I GGNCC 2 cut(s) 190, 533
BmiI GGNNCC 5 cut(s) 65, 504, 534, 535, 558
BmrFI CCNGG 4 cut(s) 188, 208, 344, 544
BmsI GCATC 3 cut(s) 15, 220, 456
BpiI GAAGAC 1 cut(s) 756
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 1 cut(s) 692
BpuEI CTTGAG 1 cut(s) 20
BpuMI CCSGG 2 cut(s) 344, 544
BsaAI YACGTR 1 cut(s) 323
BsaHI GRCGYC 2 cut(s) 184, 548
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 4 cut(s) 59, 231, 537, 552
Bse1I ACTGG 1 cut(s) 659
BseBI CCWGG 2 cut(s) 188, 208
BseDI CCNNGG 4 cut(s) 59, 231, 537, 552
BseGI GGATG 3 cut(s) 46, 352, 397
BseMII CTCAG 3 cut(s) 249, 452, 648
BseNI ACTGG 1 cut(s) 659
BseRI GAGGAG 1 cut(s) 23
BseX3I CGGCCG 1 cut(s) 540
Bsh1236I CGCG 1 cut(s) 539
Bsh1285I CGRYCG 3 cut(s) 55, 334, 543
BshFI GGCC 5 cut(s) 58, 191, 206, 342, 542
BsiEI CGRYCG 3 cut(s) 55, 334, 543
BsiHKCI CYCGRG 2 cut(s) 278, 518
BsiSI CCGG 3 cut(s) 343, 490, 543
BslFI GGGAC 3 cut(s) 519, 559, 622
BsmAI GTCTC 2 cut(s) 41, 706
BsmBI CGTCTC 1 cut(s) 706
BsmFI GGGAC 3 cut(s) 519, 559, 622
BsmI GAATGC 1 cut(s) 368
BsnI GGCC 5 cut(s) 58, 191, 206, 342, 542
Bso31I GGTCTC 1 cut(s) 41
BsoBI CYCGRG 2 cut(s) 278, 518
Bsp1286I GDGCHC 2 cut(s) 285, 625
Bsp143I GATC 2 cut(s) 52, 704
Bsp19I CCATGG 1 cut(s) 59
BspACI CCGC 8 cut(s) 203, 297, 326, 330, 500, 515, 537, 539
BspANI GGCC 5 cut(s) 58, 191, 206, 342, 542
BspCNI CTCAG 3 cut(s) 250, 453, 647
BspFNI CGCG 1 cut(s) 539
BspLI GGNNCC 5 cut(s) 65, 504, 534, 535, 558
BspMI ACCTGC 1 cut(s) 485
BspTNI GGTCTC 1 cut(s) 41
BsrBI CCGCTC 2 cut(s) 203, 517
BsrI ACTGG 1 cut(s) 659
BssECI CCNNGG 4 cut(s) 59, 231, 537, 552
BssMI GATC 2 cut(s) 52, 704
BssNI GRCGYC 2 cut(s) 184, 548
BssSI CACGAG 1 cut(s) 525
BssT1I CCWWGG 1 cut(s) 59
Bst2BI CACGAG 1 cut(s) 525
Bst2UI CCWGG 2 cut(s) 188, 208
Bst4CI ACNGT 2 cut(s) 232, 408
BstACI GRCGYC 2 cut(s) 184, 548
BstBAI YACGTR 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 328, 425
BstDEI CTNAG 5 cut(s) 258, 461, 634, 692, 754
BstDSI CCRYGG 4 cut(s) 59, 231, 537, 552
BstENI CCTNNNNNAGG 2 cut(s) 35, 166
BstF5I GGATG 3 cut(s) 46, 352, 397
BstFNI CGCG 1 cut(s) 539
BstHHI GCGC 2 cut(s) 30, 368
BstKTI GATC 2 cut(s) 55, 707
BstMAI GTCTC 2 cut(s) 41, 706
BstMBI GATC 2 cut(s) 52, 704
BstMCI CGRYCG 3 cut(s) 55, 334, 543
BstMWI GCNNNNNNNGC 3 cut(s) 486, 511, 520
BstNI CCWGG 2 cut(s) 188, 208
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 4 cut(s) 186, 206, 342, 542
BstUI CGCG 1 cut(s) 539
BstV2I GAAGAC 1 cut(s) 756
BstX2I RGATCY 1 cut(s) 704
BstYI RGATCY 1 cut(s) 704
BstZI CGGCCG 1 cut(s) 540
BsuRI GGCC 5 cut(s) 58, 191, 206, 342, 542
BtgI CCRYGG 4 cut(s) 59, 231, 537, 552
BtgZI GCGATG 1 cut(s) 277
BtrI CACGTC 1 cut(s) 509
BtsCI GGATG 3 cut(s) 46, 352, 397
BveI ACCTGC 1 cut(s) 485
Cac8I GCNNGC 2 cut(s) 328, 425
CfoI GCGC 2 cut(s) 30, 368
Cfr13I GGNCC 2 cut(s) 190, 533
Cfr42I CCGCGG 1 cut(s) 540
CseI GACGC 3 cut(s) 163, 192, 556
Csp6I GTAC 1 cut(s) 642
CviAII CATG 6 cut(s) 60, 239, 437, 572, 597, 697
CviQI GTAC 1 cut(s) 642
DdeI CTNAG 5 cut(s) 258, 461, 634, 692, 754
DpnI GATC 2 cut(s) 54, 706
DpnII GATC 2 cut(s) 52, 704
EaeI YGGCCR 3 cut(s) 56, 340, 540
EagI CGGCCG 1 cut(s) 540
EciI GGCGGA 1 cut(s) 515
EclXI CGGCCG 1 cut(s) 540
Eco130I CCWWGG 1 cut(s) 59
Eco24I GRGCYC 2 cut(s) 285, 625
Eco31I GGTCTC 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 533
Eco52I CGGCCG 1 cut(s) 540
Eco72I CACGTG 1 cut(s) 323
Eco88I CYCGRG 2 cut(s) 278, 518
EcoNI CCTNNNNNAGG 2 cut(s) 35, 166
EcoO109I RGGNCCY 2 cut(s) 190, 533
EcoRI GAATTC 1 cut(s) 577
EcoRII CCWGG 2 cut(s) 186, 206
EcoT14I CCWWGG 1 cut(s) 59
EcoT38I GRGCYC 2 cut(s) 285, 625
ErhI CCWWGG 1 cut(s) 59
Esp3I CGTCTC 1 cut(s) 706
FaeI CATG 6 cut(s) 63, 242, 440, 575, 600, 700
FaqI GGGAC 3 cut(s) 519, 559, 622
FatI CATG 6 cut(s) 59, 238, 436, 571, 596, 696
FauI CCCGC 2 cut(s) 319, 544
FblI GTMKAC 1 cut(s) 14
Fnu4HI GCNGC 4 cut(s) 204, 297, 515, 540
FokI GGATG 3 cut(s) 53, 359, 404
FriOI GRGCYC 2 cut(s) 285, 625
Fsp4HI GCNGC 4 cut(s) 204, 297, 515, 540
FspBI CTAG 1 cut(s) 143
GlaI GCGC 2 cut(s) 29, 367
GluI GCNGC 4 cut(s) 204, 297, 515, 540
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 5 cut(s) 58, 191, 206, 342, 542
HapII CCGG 3 cut(s) 343, 490, 543
HgaI GACGC 3 cut(s) 163, 192, 556
HhaI GCGC 2 cut(s) 30, 368
Hin1I GRCGYC 2 cut(s) 184, 548
Hin1II CATG 6 cut(s) 63, 242, 440, 575, 600, 700
Hin6I GCGC 2 cut(s) 28, 366
HinP1I GCGC 2 cut(s) 28, 366
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HinfI GANTC 3 cut(s) 254, 276, 687
HpaII CCGG 3 cut(s) 343, 490, 543
Hpy166II GTNNAC 2 cut(s) 15, 228
Hpy188I TCNGA 5 cut(s) 24, 182, 259, 616, 683
Hpy188III TCNNGA 3 cut(s) 335, 711, 734
Hpy8I GTNNAC 2 cut(s) 15, 228
Hpy99I CGWCG 2 cut(s) 134, 186
HpyAV CCTTC 3 cut(s) 203, 599, 611
HpyCH4III ACNGT 2 cut(s) 232, 408
HpyCH4IV ACGT 2 cut(s) 322, 508
HpyCH4V TGCA 3 cut(s) 423, 440, 447
HpyF10VI GCNNNNNNNGC 3 cut(s) 486, 511, 520
HpyF3I CTNAG 5 cut(s) 258, 461, 634, 692, 754
HpySE526I ACGT 2 cut(s) 322, 508
Hsp92I GRCGYC 2 cut(s) 184, 548
Hsp92II CATG 6 cut(s) 63, 242, 440, 575, 600, 700
HspAI GCGC 2 cut(s) 28, 366
KflI GGGWCCC 1 cut(s) 533
KspI CCGCGG 1 cut(s) 540
Kzo9I GATC 2 cut(s) 52, 704
LmnI GCTCC 4 cut(s) 502, 562, 568, 661
LweI GCATC 3 cut(s) 15, 220, 456
MaeI CTAG 1 cut(s) 143
MaeII ACGT 2 cut(s) 322, 508
MalI GATC 2 cut(s) 54, 706
MbiI CCGCTC 2 cut(s) 203, 517
MboI GATC 2 cut(s) 52, 704
MboII GAAGA 5 cut(s) 407, 696, 699, 749, 761
MflI RGATCY 1 cut(s) 704
MhlI GDGCHC 2 cut(s) 285, 625
MluCI AATT 4 cut(s) 18, 217, 418, 577
MlyI GAGTC 2 cut(s) 263, 270
MmeI TCCRAC 1 cut(s) 205
MnlI CCTC 9 cut(s) 41, 44, 188, 295, 382, 490, 523, 660, 753
MslI CAYNNNNRTG 1 cut(s) 595
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 3 cut(s) 343, 490, 543
MspR9I CCNGG 4 cut(s) 188, 208, 344, 544
Mva1269I GAATGC 1 cut(s) 368
MvaI CCWGG 2 cut(s) 188, 208
MvnI CGCG 1 cut(s) 539
MwoI GCNNNNNNNGC 3 cut(s) 486, 511, 520
NciI CCSGG 2 cut(s) 344, 544
NcoI CCATGG 1 cut(s) 59
NdeII GATC 2 cut(s) 52, 704
NlaIII CATG 6 cut(s) 63, 242, 440, 575, 600, 700
NlaIV GGNNCC 5 cut(s) 65, 504, 534, 535, 558
NmeAIII GCCGAG 1 cut(s) 224
NspI RCATGY 1 cut(s) 575
PaeR7I CTCGAG 2 cut(s) 278, 518
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 285
PctI GAATGC 1 cut(s) 368
PfeI GAWTC 1 cut(s) 687
PkrI GCNGC 4 cut(s) 205, 298, 516, 541
Ple19I CGATCG 1 cut(s) 55
PleI GAGTC 2 cut(s) 262, 270
PmaCI CACGTG 1 cut(s) 323
PmlI CACGTG 1 cut(s) 323
PpsI GAGTC 2 cut(s) 262, 270
Ppu21I YACGTR 1 cut(s) 323
PpuMI RGGWCCY 1 cut(s) 533
Psp5II RGGWCCY 1 cut(s) 533
Psp6I CCWGG 2 cut(s) 186, 206
PspCI CACGTG 1 cut(s) 323
PspGI CCWGG 2 cut(s) 186, 206
PspN4I GGNNCC 5 cut(s) 65, 504, 534, 535, 558
PspPI GGNCC 2 cut(s) 190, 533
PspPPI RGGWCCY 1 cut(s) 533
PspXI VCTCGAGB 2 cut(s) 278, 518
PsuI RGATCY 1 cut(s) 704
PvuI CGATCG 1 cut(s) 55
RsaI GTAC 1 cut(s) 643
RsaNI GTAC 1 cut(s) 642
RseI CAYNNNNRTG 1 cut(s) 595
SacII CCGCGG 1 cut(s) 540
SatI GCNGC 4 cut(s) 204, 297, 515, 540
Sau3AI GATC 2 cut(s) 52, 704
Sau96I GGNCC 2 cut(s) 190, 533
SchI GAGTC 2 cut(s) 263, 270
ScrFI CCNGG 4 cut(s) 188, 208, 344, 544
SduI GDGCHC 2 cut(s) 285, 625
SetI ASST 8 cut(s) 80, 164, 173, 325, 479, 511, 635, 671
SfaNI GCATC 3 cut(s) 15, 220, 456
Sfr274I CTCGAG 2 cut(s) 278, 518
Sfr303I CCGCGG 1 cut(s) 540
SgrBI CCGCGG 1 cut(s) 540
SinI GGWCC 1 cut(s) 533
SlaI CTCGAG 2 cut(s) 278, 518
SmiMI CAYNNNNRTG 1 cut(s) 595
SmlI CTYRAG 3 cut(s) 35, 278, 518
SmoI CTYRAG 3 cut(s) 35, 278, 518
Sse9I AATT 4 cut(s) 18, 217, 418, 577
SsiI CCGC 8 cut(s) 203, 297, 326, 330, 500, 515, 537, 539
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 4 cut(s) 186, 206, 342, 542
StyI CCWWGG 1 cut(s) 59
TaaI ACNGT 2 cut(s) 232, 408
TaiI ACGT 2 cut(s) 325, 511
TaqI TCGA 6 cut(s) 279, 334, 450, 519, 665, 710
TaqII GACCGA 1 cut(s) 65
TasI AATT 4 cut(s) 18, 217, 418, 577
TauI GCSGC 4 cut(s) 206, 299, 517, 542
TfiI GAWTC 1 cut(s) 687
TspDTI ATGAA 3 cut(s) 111, 146, 408
TspGWI ACGGA 4 cut(s) 118, 238, 277, 288
VpaK11BI GGWCC 1 cut(s) 533
XagI CCTNNNNNAGG 2 cut(s) 35, 166
XapI RAATTY 1 cut(s) 577
XceI RCATGY 1 cut(s) 575
XhoI CTCGAG 2 cut(s) 278, 518
XmiI GTMKAC 1 cut(s) 14
XspI CTAG 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.