Rh2AG334800

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015071
Physical Location & Seq
Reverse (-)
48481211 .. 48483281
2071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG334800.1

Sequence Viewer

Length: 771 bp
ATGTCTTCTTCCTCTCACACAGATCCGATCATGCGCCTTCTCAACGAAAACGATGATTTTCCGAAGCCGCTGCTCCCTTCTCATAAAGACCCGTATGACTTGAACAGCAAGATCATGCTCATCGCCATCATTGCATTATCCGTCGTGGTTGTGCTGGTAGTACTCCTTCACACCTACGCAAGATGCGTCCTCAGACGCCAGTCTCTTCAGCGAGCTGCCGCCTTACGCCGCCTGGGTTCGACTGTGGCCCATGTCCACTCTTCCGCCGAGCCACCCAAGACCGGCCTCGAGCCCTCCGTCATCGCCGCCTTGCCCGTCTTCGTATACAAGCGCGTGGAGGGTGAGGGACGTCATGAGGGCGATGCTGACGTCGAGTGTGCCGTGTGTCTGAGCATGTTGGAGGATGAAGAAATGGCGAGGTTGCTTCCCAATTGTAAGCACAGCTTCCATGCACAATGCATAGACACATGGCTGAATTCTCACACCACTTGCCCTATCTGCCGGACGGAGGCGGAGCCCCGTATAGCTCTCCAGCCAGAGCGACGGGAGGGTCCTGTGGCAGGCGCAGCAGCCACGGCTCCACCGTTGGAGCGGGTGAACTCCGTCTTGTCATGTGCGGAAGAGACATCGGAAGACGGTAAAGCAATAAATAGTGGTTCCATTTCGAGGTTGAGTAGTTCTTTCAGAAGAATGCTTAGTAGAGAAAGATCTTCCAGAAGGGTTCAATCATATGGTCAAGAAGATGGTTTTGAAGATTTAGAGAGACAGTGA

Protein Analysis

256

Amino Acids

28.29

Weight (kDa)

6.15

Isoelectric Point (pI)

64.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 125 - 168 3e-13 Ring finger domain
zf-C3HC4_2 PF13923 125 - 167 3.4e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 125 - 154 8.6e-10 RING-like zinc finger
zf-RING_5 PF14634 125 - 169 6e-06 zinc-RING finger domain
zf-rbx1 PF12678 126 - 168 5e-10 RING-H2 zinc finger domain
zf-C3HC4 PF00097 126 - 167 1.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 139 - 170 4.1e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42350 AT2G42360
fragaria_vesca FvH4_6g27024
malus_domestica MD09G1240700.v1.1 MD17G1233600.v1.1
prunus_persica Prupe.3G125000_v2.0.a1
pyrus_communis pycom09g15940 pycom17g23670
rosa_chinensis RchiOBHm_Chr2g0128891
rosa_laevigata RLG00000019039
rosa_rugosa Rorug02G0282200
rosa_samantha Rh2AG334800 Rh2BG342800 Rh2CG321800 Rh2DG359400
rosa_wichuraiana Rw2G027080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 549
AatII GACGTC 2 cut(s) 352, 372
AccBSI CCGCTC 1 cut(s) 592
AccI GTMKAC 1 cut(s) 324
AccII CGCG 1 cut(s) 333
AciI CCGC 8 cut(s) 68, 219, 229, 264, 306, 512, 592, 617
AclWI GGATC 1 cut(s) 17
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 1 cut(s) 191
AcyI GRCGYC 3 cut(s) 196, 349, 369
AfaI GTAC 1 cut(s) 162
AfiI CCNNNNNNNGG 4 cut(s) 281, 508, 560, 666
AgsI TTSAA 3 cut(s) 103, 725, 752
AjnI CCWGG 1 cut(s) 231
AluBI AGCT 3 cut(s) 215, 444, 527
AluI AGCT 3 cut(s) 215, 444, 527
Alw26I GTCTC 3 cut(s) 207, 617, 757
AlwI GGATC 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 2 cut(s) 246, 283
ApeKI GCWGC 4 cut(s) 70, 215, 566, 569
ApoI RAATTY 1 cut(s) 475
AspLEI GCGC 3 cut(s) 36, 333, 566
AspS9I GGNCC 2 cut(s) 247, 551
AsuHPI GGTGA 2 cut(s) 353, 607
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 551
BanII GRGCYC 2 cut(s) 294, 519
BarI GAAGNNNNNNTAC 2 cut(s) 150, 182
BbsI GAAGAC 2 cut(s) 310, 639
BbvI GCAGC 4 cut(s) 57, 202, 578, 581
BccI CCATC 2 cut(s) 134, 737
BceAI ACGGC 2 cut(s) 365, 591
BcgI CGANNNNNNTGC 2 cut(s) 52, 86
BciT130I CCWGG 1 cut(s) 233
BcoDI GTCTC 3 cut(s) 207, 617, 757
BglII AGATCT 1 cut(s) 707
BisI GCNGC 8 cut(s) 68, 71, 216, 219, 229, 306, 567, 570
BlsI GCNGC 8 cut(s) 69, 72, 217, 220, 230, 307, 568, 571
BmcAI AGTACT 1 cut(s) 162
Bme1390I CCNGG 1 cut(s) 233
Bme18I GGWCC 1 cut(s) 551
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 2 cut(s) 247, 551
BmiI GGNNCC 4 cut(s) 516, 552, 579, 658
BmrFI CCNGG 1 cut(s) 233
BmsI GCATC 2 cut(s) 173, 352
BoxI GACNNNNGTC 1 cut(s) 199
BpiI GAAGAC 2 cut(s) 310, 639
BpmI CTGGAG 1 cut(s) 515
BsaHI GRCGYC 3 cut(s) 196, 349, 369
BsaJI CCNNGG 2 cut(s) 232, 573
Bsc4I CCNNNNNNNGG 4 cut(s) 281, 508, 560, 666
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 1 cut(s) 129
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 232, 573
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 4 cut(s) 281, 508, 560, 666
BseMI GCAATG 1 cut(s) 129
BseMII CTCAG 2 cut(s) 205, 380
BseNI ACTGG 1 cut(s) 199
BseXI GCAGC 4 cut(s) 57, 202, 578, 581
Bsh1236I CGCG 1 cut(s) 333
BshFI GGCC 2 cut(s) 248, 285
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 2 cut(s) 282, 502
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 4 cut(s) 281, 508, 560, 666
BsmAI GTCTC 3 cut(s) 207, 617, 757
BsmFI GGGAC 1 cut(s) 360
BsmI GAATGC 1 cut(s) 696
BsnI GGCC 2 cut(s) 248, 285
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 2 cut(s) 294, 519
Bsp143I GATC 4 cut(s) 22, 27, 111, 707
BspACI CCGC 8 cut(s) 68, 219, 229, 264, 306, 512, 592, 617
BspANI GGCC 2 cut(s) 248, 285
BspCNI CTCAG 2 cut(s) 204, 381
BspFNI CGCG 1 cut(s) 333
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 4 cut(s) 516, 552, 579, 658
BspPI GGATC 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 592
BsrDI GCAATG 1 cut(s) 129
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 2 cut(s) 232, 573
BssMI GATC 4 cut(s) 22, 27, 111, 707
BssNAI GTATAC 1 cut(s) 325
BssNI GRCGYC 3 cut(s) 196, 349, 369
Bst1107I GTATAC 1 cut(s) 325
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 4 cut(s) 244, 585, 638, 768
Bst6I CTCTTC 3 cut(s) 210, 265, 615
BstACI GRCGYC 3 cut(s) 196, 349, 369
BstC8I GCNNGC 2 cut(s) 213, 562
BstDEI CTNAG 3 cut(s) 191, 389, 695
BstDSI CCRYGG 1 cut(s) 573
BstENI CCTNNNNNAGG 1 cut(s) 558
BstF5I GGATG 1 cut(s) 409
BstFNI CGCG 1 cut(s) 333
BstHHI GCGC 3 cut(s) 36, 333, 566
BstKTI GATC 4 cut(s) 25, 30, 114, 710
BstMAI GTCTC 3 cut(s) 207, 617, 757
BstMBI GATC 4 cut(s) 22, 27, 111, 707
BstMWI GCNNNNNNNGC 4 cut(s) 131, 498, 566, 575
BstNI CCWGG 1 cut(s) 233
BstNSI RCATGY 1 cut(s) 397
BstPAI GACNNNNGTC 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 231
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 4 cut(s) 57, 202, 578, 581
BstV2I GAAGAC 2 cut(s) 310, 639
BstX2I RGATCY 2 cut(s) 22, 707
BstYI RGATCY 2 cut(s) 22, 707
BstZ17I GTATAC 1 cut(s) 325
BsuRI GGCC 2 cut(s) 248, 285
BtgI CCRYGG 1 cut(s) 573
BtgZI GCGATG 3 cut(s) 106, 286, 375
BtsCI GGATG 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 213, 562
CciI TCATGA 1 cut(s) 352
CfoI GCGC 3 cut(s) 36, 333, 566
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 2 cut(s) 247, 551
CseI GACGC 2 cut(s) 175, 204
Csp6I GTAC 1 cut(s) 161
CviAII CATG 8 cut(s) 31, 115, 251, 353, 394, 449, 468, 612
CviQI GTAC 1 cut(s) 161
DdeI CTNAG 3 cut(s) 191, 389, 695
DpnI GATC 4 cut(s) 24, 29, 113, 709
DpnII GATC 4 cut(s) 22, 27, 111, 707
DrdI GACNNNNNNGTC 1 cut(s) 549
DseDI GACNNNNNNGTC 1 cut(s) 549
Eam1104I CTCTTC 3 cut(s) 210, 265, 615
EarI CTCTTC 3 cut(s) 210, 265, 615
EciI GGCGGA 2 cut(s) 253, 527
Eco24I GRGCYC 2 cut(s) 294, 519
Eco47I GGWCC 1 cut(s) 551
Eco57I CTGAAG 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 287
EcoNI CCTNNNNNAGG 1 cut(s) 558
EcoO109I RGGNCCY 1 cut(s) 551
EcoRI GAATTC 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 231
EcoT22I ATGCAT 1 cut(s) 461
EcoT38I GRGCYC 2 cut(s) 294, 519
FaeI CATG 8 cut(s) 34, 118, 254, 356, 397, 452, 471, 615
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FaqI GGGAC 1 cut(s) 360
FatI CATG 8 cut(s) 30, 114, 250, 352, 393, 448, 467, 611
FauI CCCGC 1 cut(s) 585
FauNDI CATATG 1 cut(s) 730
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 8 cut(s) 68, 71, 216, 219, 229, 306, 567, 570
FokI GGATG 1 cut(s) 416
FriOI GRGCYC 2 cut(s) 294, 519
Fsp4HI GCNGC 8 cut(s) 68, 71, 216, 219, 229, 306, 567, 570
GlaI GCGC 3 cut(s) 35, 332, 565
GluI GCNGC 8 cut(s) 68, 71, 216, 219, 229, 306, 567, 570
GsuI CTGGAG 1 cut(s) 515
HaeIII GGCC 2 cut(s) 248, 285
HapII CCGG 2 cut(s) 282, 502
HgaI GACGC 2 cut(s) 175, 204
HhaI GCGC 3 cut(s) 36, 333, 566
Hin1I GRCGYC 3 cut(s) 196, 349, 369
Hin1II CATG 8 cut(s) 34, 118, 254, 356, 397, 452, 471, 615
Hin6I GCGC 3 cut(s) 34, 331, 564
HinP1I GCGC 3 cut(s) 34, 331, 564
HpaII CCGG 2 cut(s) 282, 502
HphI GGTGA 2 cut(s) 353, 607
Hpy166II GTNNAC 3 cut(s) 256, 325, 598
Hpy188I TCNGA 6 cut(s) 27, 63, 194, 390, 631, 686
Hpy188III TCNNGA 3 cut(s) 353, 714, 737
Hpy8I GTNNAC 3 cut(s) 256, 325, 598
Hpy99I CGWCG 3 cut(s) 146, 374, 546
HpyAV CCTTC 4 cut(s) 47, 87, 176, 711
HpyCH4III ACNGT 4 cut(s) 244, 585, 638, 768
HpyCH4IV ACGT 2 cut(s) 349, 369
HpyCH4V TGCA 3 cut(s) 134, 452, 459
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 498, 566, 575
HpyF3I CTNAG 3 cut(s) 191, 389, 695
HpySE526I ACGT 2 cut(s) 349, 369
Hsp92I GRCGYC 3 cut(s) 196, 349, 369
Hsp92II CATG 8 cut(s) 34, 118, 254, 356, 397, 452, 471, 615
HspAI GCGC 3 cut(s) 34, 331, 564
Kzo9I GATC 4 cut(s) 22, 27, 111, 707
LmnI GCTCC 4 cut(s) 78, 514, 583, 589
Lsp1109I GCAGC 4 cut(s) 57, 202, 578, 581
LweI GCATC 2 cut(s) 173, 352
MaeII ACGT 2 cut(s) 349, 369
MalI GATC 4 cut(s) 24, 29, 113, 709
MbiI CCGCTC 1 cut(s) 592
MboI GATC 4 cut(s) 22, 27, 111, 707
MfeI CAATTG 1 cut(s) 430
MflI RGATCY 2 cut(s) 22, 707
MhlI GDGCHC 2 cut(s) 294, 519
MluCI AATT 2 cut(s) 430, 475
MmeI TCCRAC 2 cut(s) 378, 567
Mph1103I ATGCAT 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 70
MspI CCGG 2 cut(s) 282, 502
MspR9I CCNGG 1 cut(s) 233
MunI CAATTG 1 cut(s) 430
Mva1269I GAATGC 1 cut(s) 696
MvaI CCWGG 1 cut(s) 233
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 4 cut(s) 131, 498, 566, 575
NdeI CATATG 1 cut(s) 730
NdeII GATC 4 cut(s) 22, 27, 111, 707
NlaIII CATG 8 cut(s) 34, 118, 254, 356, 397, 452, 471, 615
NlaIV GGNNCC 4 cut(s) 516, 552, 579, 658
NmeAIII GCCGAG 1 cut(s) 292
NsiI ATGCAT 1 cut(s) 461
NspI RCATGY 1 cut(s) 397
PaeR7I CTCGAG 1 cut(s) 287
PagI TCATGA 1 cut(s) 352
PcsI WCGNNNNNNNCGW 4 cut(s) 183, 294, 378, 581
PctI GAATGC 1 cut(s) 696
PkrI GCNGC 8 cut(s) 69, 72, 217, 220, 230, 307, 568, 571
PpuMI RGGWCCY 1 cut(s) 551
PshAI GACNNNNGTC 1 cut(s) 199
Psp5II RGGWCCY 1 cut(s) 551
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 4 cut(s) 516, 552, 579, 658
PspPI GGNCC 2 cut(s) 247, 551
PspPPI RGGWCCY 1 cut(s) 551
PspXI VCTCGAGB 1 cut(s) 287
PsuI RGATCY 2 cut(s) 22, 707
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
SatI GCNGC 8 cut(s) 68, 71, 216, 219, 229, 306, 567, 570
Sau3AI GATC 4 cut(s) 22, 27, 111, 707
Sau96I GGNCC 2 cut(s) 247, 551
ScaI AGTACT 1 cut(s) 162
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 2 cut(s) 294, 519
SetI ASST 8 cut(s) 176, 217, 352, 372, 422, 446, 529, 671
SfaNI GCATC 2 cut(s) 173, 352
Sfr274I CTCGAG 1 cut(s) 287
SinI GGWCC 1 cut(s) 551
SlaI CTCGAG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 2 cut(s) 430, 475
SsiI CCGC 8 cut(s) 68, 219, 229, 264, 306, 512, 592, 617
StyD4I CCNGG 1 cut(s) 231
TaaI ACNGT 4 cut(s) 244, 585, 638, 768
TaiI ACGT 2 cut(s) 352, 372
TaqI TCGA 4 cut(s) 239, 288, 372, 665
TasI AATT 2 cut(s) 430, 475
TatI WGTACW 1 cut(s) 160
TauI GCSGC 4 cut(s) 70, 221, 231, 308
TseI GCWGC 4 cut(s) 70, 215, 566, 569
TspDTI ATGAA 1 cut(s) 420
TspGWI ACGGA 4 cut(s) 130, 286, 521, 592
VpaK11BI GGWCC 1 cut(s) 551
XagI CCTNNNNNAGG 1 cut(s) 558
XapI RAATTY 1 cut(s) 475
XceI RCATGY 1 cut(s) 397
XhoI CTCGAG 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 324
ZraI GACGTC 2 cut(s) 350, 370
ZrmI AGTACT 1 cut(s) 162
Zsp2I ATGCAT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.