Rw2G027080

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015071
Physical Location & Seq
Reverse (-)
42141996 .. 42143153
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027080.1

Sequence Viewer

Length: 768 bp
ATGTCCTCCTCTCACACAGATCCGATCATGCGCCTTCTCGACGAAAGCGATGATAATCCGAAGAAGTCGTTCCCTTCTCATAATGACCCGTATGACTTGAACAGCAAGATCATGCTCATCGCCATCATTGCATTATCCGTCGTGGTTGTGCTGGTAGTACTCCTTCACACCTACGCAAGATGCGTCCTCAGACGCCAGTCTCTTCAGCGAGCTGCCGCCTTACGCCGCCTGGGTTCGACTGTGGCCCATGTCCACTCTTCTGCCGAGCCACCCAAGACCGGCCTCGAGCCCTCCGTCATCGCCGCCTTGCCCGTCTTCGTATACAAGCGCGTGGAGGGTGAGGGACGTCATGAGGGCGATGCTGATGTCGAGTGTGCCGTGTGTCTGAGCATGTTGGAGGATGAAGAAATGGCGAGGTTGCTTCCCAATTGTAAGCACAGCTTCCATGCACAATGCATAGACACGTGGCTGAATTCTCACACCACTTGCCCTATCTGCCGGACGGAGGCGGAGCCCCGTATAGCTCTCCAGCCGGAGCGACGGGAGGGTCCTGTGGCCGGCGCAGTAGCCACGGCTCCACCGTTGGAGCGGGTGAACTCCGTCTTGTCATGTGCGGAAGAGACATCGGAAGACGGTAAAGCAATAAATAGTGGTTCCATTTCGAGGTTGAGTAGTTCTTTCAGAAGAATGCTTAGTAGAGAAAGATCTTCCAGAAGGGTTCAATCATATGGTCAAGAAGAAGGTTTTGAAGATTTAGAGAGACAGTGA

Protein Analysis

255

Amino Acids

28.22

Weight (kDa)

6.01

Isoelectric Point (pI)

64.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 124 - 167 3e-13 Ring finger domain
zf-C3HC4_2 PF13923 124 - 166 3.4e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 124 - 153 8.5e-10 RING-like zinc finger
zf-RING_5 PF14634 124 - 168 6e-06 zinc-RING finger domain
zf-rbx1 PF12678 125 - 167 4.9e-10 RING-H2 zinc finger domain
zf-C3HC4 PF00097 125 - 166 1.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 138 - 169 4.1e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42350 AT2G42360
fragaria_vesca FvH4_6g27024
malus_domestica MD09G1240700.v1.1 MD17G1233600.v1.1
prunus_persica Prupe.3G125000_v2.0.a1
pyrus_communis pycom09g15940 pycom17g23670
rosa_chinensis RchiOBHm_Chr2g0128891
rosa_laevigata RLG00000019039
rosa_rugosa Rorug02G0282200
rosa_samantha Rh2AG334800 Rh2BG342800 Rh2CG321800 Rh2DG359400
rosa_wichuraiana Rw2G027080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 546
AatII GACGTC 1 cut(s) 349
AccBSI CCGCTC 1 cut(s) 589
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 330
AciI CCGC 6 cut(s) 216, 226, 303, 509, 589, 614
AclWI GGATC 1 cut(s) 14
AcoI YGGCCR 1 cut(s) 555
AcsI RAATTY 1 cut(s) 472
AcuI CTGAAG 1 cut(s) 188
AcvI CACGTG 1 cut(s) 465
AcyI GRCGYC 2 cut(s) 193, 346
AfaI GTAC 1 cut(s) 159
AfiI CCNNNNNNNGG 4 cut(s) 278, 505, 557, 663
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 3 cut(s) 100, 722, 749
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 3 cut(s) 212, 441, 524
AluI AGCT 3 cut(s) 212, 441, 524
Alw26I GTCTC 3 cut(s) 204, 614, 754
AlwI GGATC 1 cut(s) 14
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 3 cut(s) 243, 280, 555
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 1 cut(s) 472
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 3 cut(s) 33, 330, 563
AspS9I GGNCC 2 cut(s) 244, 548
AsuHPI GGTGA 2 cut(s) 350, 604
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 1 cut(s) 548
BanII GRGCYC 2 cut(s) 291, 516
BarI GAAGNNNNNNTAC 2 cut(s) 147, 179
BbrPI CACGTG 1 cut(s) 465
BbsI GAAGAC 2 cut(s) 307, 636
BbvI GCAGC 1 cut(s) 199
BccI CCATC 1 cut(s) 131
BceAI ACGGC 2 cut(s) 362, 588
BciT130I CCWGG 1 cut(s) 230
BcoDI GTCTC 3 cut(s) 204, 614, 754
BglII AGATCT 1 cut(s) 704
BisI GCNGC 4 cut(s) 213, 216, 226, 303
BlsI GCNGC 4 cut(s) 214, 217, 227, 304
BmcAI AGTACT 1 cut(s) 159
Bme1390I CCNGG 1 cut(s) 230
Bme18I GGWCC 1 cut(s) 548
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 2 cut(s) 244, 548
BmiI GGNNCC 4 cut(s) 513, 549, 576, 655
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 2 cut(s) 170, 349
BoxI GACNNNNGTC 1 cut(s) 196
BpiI GAAGAC 2 cut(s) 307, 636
BpmI CTGGAG 1 cut(s) 512
BsaAI YACGTR 1 cut(s) 465
BsaBI GATNNNNATC 1 cut(s) 54
BsaHI GRCGYC 2 cut(s) 193, 346
BsaJI CCNNGG 2 cut(s) 229, 570
Bsc4I CCNNNNNNNGG 4 cut(s) 278, 505, 557, 663
Bse118I RCCGGY 2 cut(s) 278, 557
Bse1I ACTGG 1 cut(s) 196
Bse3DI GCAATG 1 cut(s) 126
Bse8I GATNNNNATC 1 cut(s) 54
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 229, 570
BseGI GGATG 1 cut(s) 406
BseJI GATNNNNATC 1 cut(s) 54
BseLI CCNNNNNNNGG 4 cut(s) 278, 505, 557, 663
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 2 cut(s) 202, 377
BseNI ACTGG 1 cut(s) 196
BseXI GCAGC 1 cut(s) 199
Bsh1236I CGCG 1 cut(s) 330
BshFI GGCC 3 cut(s) 245, 282, 557
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 4 cut(s) 279, 499, 533, 558
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 4 cut(s) 278, 505, 557, 663
BsmAI GTCTC 3 cut(s) 204, 614, 754
BsmFI GGGAC 1 cut(s) 357
BsmI GAATGC 1 cut(s) 693
BsnI GGCC 3 cut(s) 245, 282, 557
BsoBI CYCGRG 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 291, 516
Bsp143I GATC 4 cut(s) 19, 24, 108, 704
BspACI CCGC 6 cut(s) 216, 226, 303, 509, 589, 614
BspANI GGCC 3 cut(s) 245, 282, 557
BspCNI CTCAG 2 cut(s) 201, 378
BspFNI CGCG 1 cut(s) 330
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 4 cut(s) 513, 549, 576, 655
BspPI GGATC 1 cut(s) 14
BsrBI CCGCTC 1 cut(s) 589
BsrDI GCAATG 1 cut(s) 126
BsrFI RCCGGY 2 cut(s) 278, 557
BsrI ACTGG 1 cut(s) 196
BssAI RCCGGY 2 cut(s) 278, 557
BssECI CCNNGG 2 cut(s) 229, 570
BssMI GATC 4 cut(s) 19, 24, 108, 704
BssNAI GTATAC 1 cut(s) 322
BssNI GRCGYC 2 cut(s) 193, 346
Bst1107I GTATAC 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 4 cut(s) 241, 582, 635, 765
Bst6I CTCTTC 3 cut(s) 207, 262, 612
BstACI GRCGYC 2 cut(s) 193, 346
BstBAI YACGTR 1 cut(s) 465
BstC8I GCNNGC 2 cut(s) 210, 559
BstDEI CTNAG 3 cut(s) 188, 386, 692
BstDSI CCRYGG 1 cut(s) 570
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 1 cut(s) 330
BstHHI GCGC 3 cut(s) 33, 330, 563
BstKTI GATC 4 cut(s) 22, 27, 111, 707
BstMAI GTCTC 3 cut(s) 204, 614, 754
BstMBI GATC 4 cut(s) 19, 24, 108, 704
BstMWI GCNNNNNNNGC 2 cut(s) 128, 495
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 394
BstPAI GACNNNNGTC 1 cut(s) 196
BstSCI CCNGG 1 cut(s) 228
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 1 cut(s) 199
BstV2I GAAGAC 2 cut(s) 307, 636
BstX2I RGATCY 2 cut(s) 19, 704
BstYI RGATCY 2 cut(s) 19, 704
BstZ17I GTATAC 1 cut(s) 322
BsuRI GGCC 3 cut(s) 245, 282, 557
BtgI CCRYGG 1 cut(s) 570
BtgZI GCGATG 4 cut(s) 63, 103, 283, 372
BtsCI GGATG 1 cut(s) 406
Cac8I GCNNGC 2 cut(s) 210, 559
CciI TCATGA 1 cut(s) 349
CfoI GCGC 3 cut(s) 33, 330, 563
Cfr10I RCCGGY 2 cut(s) 278, 557
Cfr13I GGNCC 2 cut(s) 244, 548
CseI GACGC 2 cut(s) 172, 201
Csp6I GTAC 1 cut(s) 158
CviAII CATG 7 cut(s) 28, 112, 248, 350, 391, 446, 609
CviQI GTAC 1 cut(s) 158
DdeI CTNAG 3 cut(s) 188, 386, 692
DpnI GATC 4 cut(s) 21, 26, 110, 706
DpnII GATC 4 cut(s) 19, 24, 108, 704
DrdI GACNNNNNNGTC 1 cut(s) 546
DseDI GACNNNNNNGTC 1 cut(s) 546
EaeI YGGCCR 1 cut(s) 555
Eam1104I CTCTTC 3 cut(s) 207, 262, 612
EarI CTCTTC 3 cut(s) 207, 262, 612
EciI GGCGGA 1 cut(s) 524
Eco24I GRGCYC 2 cut(s) 291, 516
Eco47I GGWCC 1 cut(s) 548
Eco57I CTGAAG 1 cut(s) 188
Eco72I CACGTG 1 cut(s) 465
Eco88I CYCGRG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 548
EcoRI GAATTC 1 cut(s) 472
EcoRII CCWGG 1 cut(s) 228
EcoT22I ATGCAT 1 cut(s) 458
EcoT38I GRGCYC 2 cut(s) 291, 516
FaeI CATG 7 cut(s) 31, 115, 251, 353, 394, 449, 612
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 1 cut(s) 357
FatI CATG 7 cut(s) 27, 111, 247, 349, 390, 445, 608
FauI CCCGC 1 cut(s) 582
FauNDI CATATG 1 cut(s) 727
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 4 cut(s) 213, 216, 226, 303
FokI GGATG 1 cut(s) 413
FriOI GRGCYC 2 cut(s) 291, 516
Fsp4HI GCNGC 4 cut(s) 213, 216, 226, 303
GlaI GCGC 3 cut(s) 32, 329, 562
GluI GCNGC 4 cut(s) 213, 216, 226, 303
GsuI CTGGAG 1 cut(s) 512
HaeIII GGCC 3 cut(s) 245, 282, 557
HapII CCGG 4 cut(s) 279, 499, 533, 558
HgaI GACGC 2 cut(s) 172, 201
HhaI GCGC 3 cut(s) 33, 330, 563
Hin1I GRCGYC 2 cut(s) 193, 346
Hin1II CATG 7 cut(s) 31, 115, 251, 353, 394, 449, 612
Hin6I GCGC 3 cut(s) 31, 328, 561
HinP1I GCGC 3 cut(s) 31, 328, 561
HpaII CCGG 4 cut(s) 279, 499, 533, 558
HphI GGTGA 2 cut(s) 350, 604
Hpy166II GTNNAC 3 cut(s) 253, 322, 595
Hpy188I TCNGA 6 cut(s) 24, 60, 191, 387, 628, 683
Hpy188III TCNNGA 4 cut(s) 38, 350, 711, 734
Hpy8I GTNNAC 3 cut(s) 253, 322, 595
Hpy99I CGWCG 3 cut(s) 44, 143, 543
HpyAV CCTTC 5 cut(s) 44, 84, 173, 708, 734
HpyCH4III ACNGT 4 cut(s) 241, 582, 635, 765
HpyCH4IV ACGT 2 cut(s) 346, 464
HpyCH4V TGCA 3 cut(s) 131, 449, 456
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 495
HpyF3I CTNAG 3 cut(s) 188, 386, 692
HpySE526I ACGT 2 cut(s) 346, 464
Hsp92I GRCGYC 2 cut(s) 193, 346
Hsp92II CATG 7 cut(s) 31, 115, 251, 353, 394, 449, 612
HspAI GCGC 3 cut(s) 31, 328, 561
KroI GCCGGC 1 cut(s) 557
KroNI GCCGGC 1 cut(s) 559
Kzo9I GATC 4 cut(s) 19, 24, 108, 704
LmnI GCTCC 4 cut(s) 511, 535, 580, 586
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 2 cut(s) 170, 349
MaeII ACGT 2 cut(s) 346, 464
MalI GATC 4 cut(s) 21, 26, 110, 706
MbiI CCGCTC 1 cut(s) 589
MboI GATC 4 cut(s) 19, 24, 108, 704
MfeI CAATTG 1 cut(s) 427
MflI RGATCY 2 cut(s) 19, 704
MhlI GDGCHC 2 cut(s) 291, 516
MluCI AATT 2 cut(s) 427, 472
MmeI TCCRAC 2 cut(s) 375, 564
Mph1103I ATGCAT 1 cut(s) 458
MroNI GCCGGC 1 cut(s) 557
MroXI GAANNNNTTC 1 cut(s) 68
MspI CCGG 4 cut(s) 279, 499, 533, 558
MspR9I CCNGG 1 cut(s) 230
MunI CAATTG 1 cut(s) 427
Mva1269I GAATGC 1 cut(s) 693
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 2 cut(s) 128, 495
NaeI GCCGGC 1 cut(s) 559
NdeI CATATG 1 cut(s) 727
NdeII GATC 4 cut(s) 19, 24, 108, 704
NgoMIV GCCGGC 1 cut(s) 557
NlaIII CATG 7 cut(s) 31, 115, 251, 353, 394, 449, 612
NlaIV GGNNCC 4 cut(s) 513, 549, 576, 655
NmeAIII GCCGAG 1 cut(s) 289
NsiI ATGCAT 1 cut(s) 458
NspI RCATGY 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 284
PagI TCATGA 1 cut(s) 349
PcsI WCGNNNNNNNCGW 5 cut(s) 45, 180, 291, 375, 578
PctI GAATGC 1 cut(s) 693
PdiI GCCGGC 1 cut(s) 559
PdmI GAANNNNTTC 1 cut(s) 68
PkrI GCNGC 4 cut(s) 214, 217, 227, 304
PmaCI CACGTG 1 cut(s) 465
PmlI CACGTG 1 cut(s) 465
Ppu21I YACGTR 1 cut(s) 465
PpuMI RGGWCCY 1 cut(s) 548
PshAI GACNNNNGTC 1 cut(s) 196
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 228
PspCI CACGTG 1 cut(s) 465
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 4 cut(s) 513, 549, 576, 655
PspPI GGNCC 2 cut(s) 244, 548
PspPPI RGGWCCY 1 cut(s) 548
PspXI VCTCGAGB 1 cut(s) 284
PsuI RGATCY 2 cut(s) 19, 704
RsaI GTAC 1 cut(s) 159
RsaNI GTAC 1 cut(s) 158
SatI GCNGC 4 cut(s) 213, 216, 226, 303
Sau3AI GATC 4 cut(s) 19, 24, 108, 704
Sau96I GGNCC 2 cut(s) 244, 548
ScaI AGTACT 1 cut(s) 159
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 2 cut(s) 291, 516
SetI ASST 9 cut(s) 173, 214, 349, 419, 443, 467, 526, 668, 745
SfaNI GCATC 2 cut(s) 170, 349
Sfr274I CTCGAG 1 cut(s) 284
SinI GGWCC 1 cut(s) 548
SlaI CTCGAG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 2 cut(s) 427, 472
SsiI CCGC 6 cut(s) 216, 226, 303, 509, 589, 614
StyD4I CCNGG 1 cut(s) 228
TaaI ACNGT 4 cut(s) 241, 582, 635, 765
TaiI ACGT 2 cut(s) 349, 467
TaqI TCGA 5 cut(s) 39, 236, 285, 369, 662
TasI AATT 2 cut(s) 427, 472
TatI WGTACW 1 cut(s) 157
TauI GCSGC 3 cut(s) 218, 228, 305
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 417
TspGWI ACGGA 4 cut(s) 127, 283, 518, 589
VpaK11BI GGWCC 1 cut(s) 548
XapI RAATTY 1 cut(s) 472
XceI RCATGY 1 cut(s) 394
XhoI CTCGAG 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 321
XmnI GAANNNNTTC 1 cut(s) 68
ZraI GACGTC 1 cut(s) 347
ZrmI AGTACT 1 cut(s) 159
Zsp2I ATGCAT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.