MD09G1251200.v1.1

YIF1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009482
Physical Location & Seq
Reverse (-)
32206199 .. 32209584
3386 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1251200.v1.1.491

Sequence Viewer

Length: 774 bp
ATGCCGACGGCATCCCCGCGGCCGAATCCGTTTGAGAATTCGTTGTACGGTGCTAGTTCCGGACTAATCCGTGGTGGATTGGGTGCATATGGAGAAAAGATTTTAGGATCGAGCTCTGAGTATGTACAAAGCAATATCAGTAGGTATTTCTCTGATCCGCAATACTACTTCCAAGTGAATGACCAGTATGTGAGGAACAAATTGAAGGTCGTTCTGTTTCCTTTTCTCCACAGGGGCCATTGGACTAGAATAACGGAGCCAGTAGGAGGCAGGCTTTCTTATAAACCCCCAATCTATGACATCAATGCACCAGACCTATATATTCCATTTATGGCATTTGGTACCTATGTGGTTCTGGCTGGCTTCTCATTGGGTCTTCAAGGAAAGTTTAGCCCTGAAGCTCTAAACTGGTTGTTTATCAAGGGCTTGCTTGGCTGGTTTATGCAAGTTGCACTACTGAAAGTATCATTGCTTTCATTGGGCGGTGGTGAGGCACCGTTGCTGGACATGGTGGCATATGCTGGGTATGCTTTCACGGGGTTGTGCTTTGCTGTACTTGGACGGATTATGTTGAGCTACTCATACTACTTTTTGATGCCTTGGACGTGTTTATGCATGGGAGTCTTCTTGGTGAAGACAATGAAGAGAGTTCTATTCGCCGAGGTGAGGAGTTATGATTCAAGCAAGAACCATTACCTGTTGCTGTTTATTGCTTTGGCTCAGTTACCTCTGTTTACATGGCTTGGCAACGTTACTGTTAATTGGCTCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YIF1

Protein Analysis

258

Amino Acids

28.91

Weight (kDa)

9.23

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YIF1 PF03878 29 - 248 8.1e-56 YIF1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 341, 493
AccII CGCG 1 cut(s) 19
AccIII TCCGGA 1 cut(s) 59
AciI CCGC 4 cut(s) 17, 19, 158, 483
AclI AACGTT 1 cut(s) 750
AclWI GGATC 2 cut(s) 115, 149
AcoI YGGCCR 1 cut(s) 20
AcsI RAATTY 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 4 cut(s) 47, 126, 343, 555
AfiI CCNNNNNNNGG 2 cut(s) 266, 666
AflIII ACRYGT 1 cut(s) 605
AgsI TTSAA 3 cut(s) 205, 380, 681
AjiI CACGTC 1 cut(s) 606
AluBI AGCT 3 cut(s) 114, 401, 576
AluI AGCT 3 cut(s) 114, 401, 576
Alw21I GWGCWC 1 cut(s) 116
AlwI GGATC 2 cut(s) 115, 149
Aor13HI TCCGGA 1 cut(s) 59
AoxI GGCC 2 cut(s) 20, 235
ApoI RAATTY 1 cut(s) 37
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
Asp718I GGTACC 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 3 cut(s) 500, 643, 676
BaeI ACNNNNGTAYC 2 cut(s) 333, 366
BanI GGYRCC 2 cut(s) 341, 493
BanII GRGCYC 1 cut(s) 116
BbsI GAAGAC 3 cut(s) 368, 616, 641
Bbv12I GWGCWC 1 cut(s) 116
BceAI ACGGC 1 cut(s) 24
BfaI CTAG 2 cut(s) 54, 246
BisI GCNGC 1 cut(s) 20
BlsI GCNGC 1 cut(s) 21
BmgBI CACGTC 1 cut(s) 606
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 4 cut(s) 236, 258, 343, 495
BmsI GCATC 2 cut(s) 20, 585
BpiI GAAGAC 3 cut(s) 368, 616, 641
BsaJI CCNNGG 4 cut(s) 17, 70, 599, 660
BsaWI WCCGGW 1 cut(s) 59
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 666
Bse1I ACTGG 3 cut(s) 184, 260, 413
Bse3DI GCAATG 1 cut(s) 467
BseAI TCCGGA 1 cut(s) 59
BseDI CCNNGG 4 cut(s) 17, 70, 599, 660
BseGI GGATG 1 cut(s) 11
BseLI CCNNNNNNNGG 2 cut(s) 266, 666
BseMI GCAATG 1 cut(s) 467
BseMII CTCAG 2 cut(s) 108, 734
BseNI ACTGG 3 cut(s) 184, 260, 413
BseRI GAGGAG 1 cut(s) 682
BseX3I CGGCCG 1 cut(s) 20
BseYI CCCAGC 1 cut(s) 521
Bsh1236I CGCG 1 cut(s) 19
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 2 cut(s) 22, 237
BshNI GGYRCC 2 cut(s) 341, 493
BsiEI CGRYCG 1 cut(s) 23
BsiHKAI GWGCWC 1 cut(s) 116
BsiSI CCGG 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 266, 666
BsnI GGCC 2 cut(s) 22, 237
Bsp1286I GDGCHC 1 cut(s) 116
Bsp13I TCCGGA 1 cut(s) 59
Bsp1407I TGTACA 1 cut(s) 124
Bsp143I GATC 2 cut(s) 107, 154
BspACI CCGC 4 cut(s) 17, 19, 158, 483
BspANI GGCC 2 cut(s) 22, 237
BspCNI CTCAG 2 cut(s) 109, 733
BspEI TCCGGA 1 cut(s) 59
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 4 cut(s) 236, 258, 343, 495
BspPI GGATC 2 cut(s) 115, 149
BspT107I GGYRCC 2 cut(s) 341, 493
BsrDI GCAATG 1 cut(s) 467
BsrGI TGTACA 1 cut(s) 124
BsrI ACTGG 3 cut(s) 184, 260, 413
BssECI CCNNGG 4 cut(s) 17, 70, 599, 660
BssMI GATC 2 cut(s) 107, 154
BssT1I CCWWGG 1 cut(s) 599
Bst4CI ACNGT 3 cut(s) 50, 498, 757
Bst6I CTCTTC 1 cut(s) 638
BstAUI TGTACA 1 cut(s) 124
BstC8I GCNNGC 3 cut(s) 272, 361, 428
BstDEI CTNAG 2 cut(s) 117, 720
BstDSI CCRYGG 2 cut(s) 17, 70
BstF5I GGATG 1 cut(s) 11
BstFNI CGCG 1 cut(s) 19
BstKTI GATC 2 cut(s) 110, 157
BstMBI GATC 2 cut(s) 107, 154
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 2 cut(s) 432, 527
BstUI CGCG 1 cut(s) 19
BstV2I GAAGAC 3 cut(s) 368, 616, 641
BstZI CGGCCG 1 cut(s) 20
BsuRI GGCC 2 cut(s) 22, 237
BtgI CCRYGG 2 cut(s) 17, 70
BtrI CACGTC 1 cut(s) 606
BtsCI GGATG 1 cut(s) 11
Cac8I GCNNGC 3 cut(s) 272, 361, 428
Cfr13I GGNCC 1 cut(s) 235
Cfr42I CCGCGG 1 cut(s) 20
Csp6I GTAC 4 cut(s) 46, 125, 342, 554
CviAII CATG 3 cut(s) 508, 616, 738
CviQI GTAC 4 cut(s) 46, 125, 342, 554
DdeI CTNAG 2 cut(s) 117, 720
DpnI GATC 2 cut(s) 109, 156
DpnII GATC 2 cut(s) 107, 154
EaeI YGGCCR 1 cut(s) 20
EagI CGGCCG 1 cut(s) 20
Eam1104I CTCTTC 1 cut(s) 638
EarI CTCTTC 1 cut(s) 638
Ecl136II GAGCTC 1 cut(s) 114
EclXI CGGCCG 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 599
Eco24I GRGCYC 1 cut(s) 116
Eco52I CGGCCG 1 cut(s) 20
Eco53kI GAGCTC 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 114
EcoRI GAATTC 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 599
EcoT22I ATGCAT 1 cut(s) 617
EcoT38I GRGCYC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 599
FaeI CATG 3 cut(s) 511, 619, 741
FatI CATG 3 cut(s) 507, 615, 737
FauI CCCGC 1 cut(s) 24
FauNDI CATATG 2 cut(s) 88, 517
Fnu4HI GCNGC 1 cut(s) 20
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 1 cut(s) 20
FspBI CTAG 2 cut(s) 54, 246
GluI GCNGC 1 cut(s) 20
GsaI CCCAGC 1 cut(s) 525
HaeIII GGCC 2 cut(s) 22, 237
HapII CCGG 1 cut(s) 60
Hin1II CATG 3 cut(s) 511, 619, 741
HinfI GANTC 3 cut(s) 25, 621, 677
HpaII CCGG 1 cut(s) 60
HphI GGTGA 3 cut(s) 500, 643, 676
Hpy166II GTNNAC 1 cut(s) 735
Hpy188I TCNGA 2 cut(s) 118, 154
Hpy188III TCNNGA 1 cut(s) 60
Hpy8I GTNNAC 1 cut(s) 735
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 3 cut(s) 50, 498, 757
HpyCH4IV ACGT 2 cut(s) 605, 750
HpyCH4V TGCA 5 cut(s) 86, 308, 445, 452, 615
HpyF10VI GCNNNNNNNGC 2 cut(s) 432, 527
HpyF3I CTNAG 2 cut(s) 117, 720
HpySE526I ACGT 2 cut(s) 605, 750
Hsp92II CATG 3 cut(s) 511, 619, 741
Kpn2I TCCGGA 1 cut(s) 59
KpnI GGTACC 1 cut(s) 345
KspI CCGCGG 1 cut(s) 20
Kzo9I GATC 2 cut(s) 107, 154
LmnI GCTCC 1 cut(s) 256
LweI GCATC 2 cut(s) 20, 585
MaeI CTAG 2 cut(s) 54, 246
MaeII ACGT 2 cut(s) 605, 750
MaeIII GTNAC 2 cut(s) 723, 751
MalI GATC 2 cut(s) 109, 156
MboI GATC 2 cut(s) 107, 154
MboII GAAGA 4 cut(s) 368, 616, 646, 655
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 3 cut(s) 37, 200, 760
MlyI GAGTC 1 cut(s) 630
MnlI CCTC 6 cut(s) 186, 260, 484, 655, 660, 738
Mph1103I ATGCAT 1 cut(s) 617
MroI TCCGGA 1 cut(s) 59
MseI TTAA 1 cut(s) 759
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 1 cut(s) 60
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 2 cut(s) 432, 527
NdeI CATATG 2 cut(s) 88, 517
NdeII GATC 2 cut(s) 107, 154
NlaIII CATG 3 cut(s) 511, 619, 741
NlaIV GGNNCC 4 cut(s) 236, 258, 343, 495
NmeAIII GCCGAG 1 cut(s) 685
NsiI ATGCAT 1 cut(s) 617
PfeI GAWTC 2 cut(s) 25, 677
PkrI GCNGC 1 cut(s) 21
PleI GAGTC 1 cut(s) 629
PpsI GAGTC 1 cut(s) 629
PsiI TTATAA 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 116
Psp1406I AACGTT 1 cut(s) 750
PspFI CCCAGC 1 cut(s) 521
PspN4I GGNNCC 4 cut(s) 236, 258, 343, 495
PspPI GGNCC 1 cut(s) 235
RsaI GTAC 4 cut(s) 47, 126, 343, 555
RsaNI GTAC 4 cut(s) 46, 125, 342, 554
SacI GAGCTC 1 cut(s) 116
SacII CCGCGG 1 cut(s) 20
SaqAI TTAA 1 cut(s) 759
SatI GCNGC 1 cut(s) 20
Sau3AI GATC 2 cut(s) 107, 154
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 1 cut(s) 630
SduI GDGCHC 1 cut(s) 116
SfaNI GCATC 2 cut(s) 20, 585
Sfr303I CCGCGG 1 cut(s) 20
SgrBI CCGCGG 1 cut(s) 20
Sse9I AATT 3 cut(s) 37, 200, 760
SsiI CCGC 4 cut(s) 17, 19, 158, 483
SspMI CTAG 2 cut(s) 54, 246
SstI GAGCTC 1 cut(s) 116
StyI CCWWGG 1 cut(s) 599
TaaI ACNGT 3 cut(s) 50, 498, 757
TaiI ACGT 2 cut(s) 608, 753
TaqI TCGA 1 cut(s) 110
TasI AATT 3 cut(s) 37, 200, 760
TatI WGTACW 2 cut(s) 124, 553
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 2 cut(s) 25, 677
Tru1I TTAA 1 cut(s) 759
Tru9I TTAA 1 cut(s) 759
TspDTI ATGAA 2 cut(s) 465, 656
TspGWI ACGGA 4 cut(s) 18, 59, 269, 577
XapI RAATTY 1 cut(s) 37
XspI CTAG 2 cut(s) 54, 246
Zsp2I ATGCAT 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.