Rorug04G0071600

YIF1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009482
Physical Location & Seq
Forward (+)
11028732 .. 11031808
3077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0071600.1

Sequence Viewer

Length: 630 bp
ATGGCGACGGAAGTAGCAGCACCGGCACCGCCGTTATCCGACGTGGAGGACAACACCACCGCCGCCGCGGCAGCAGAGACGAAGAGCAGCGGGCCCCACAAGCTCGAGAGAAAGTGGACCTTCTGGTTCGACAACCAATCTAAGCCCAAACAAAGCGGCGCTGCTTGGGGCTCCTCTCTCAAAAAAGCCTACACCTTTGAAACCGTCCAGGAGTTTTGGTGTTTGTATGATCAGATATTCAAGCCGAGCAAGTTTCCAGCAAATGCTGAGTTCCACTTGTTCAGGGCTGGGGTTGAACCGAAATGGGAGGATCCTGAATGTGCTAATGGAGGGAAGTGGACTGTCACAAGCAGCAGGAAGGCCAACCTTGATACCATGTGGCTAGAAACTTTGATGGCACTGATTGGAGAGCAATTTGATGAGGGTGATGAGATTTGTGGAGTGGTAGCAAGTGTGCGCCAGAGGCAAGACAAACTTGCATTGTGGACTAGGAATGCTGCAAACGAGGCTGCCCAGATGAGCATTGGGAGGAAGTGGAAGGAGATAATCGATGTTACTGATAAGATCACTTACAGCTTCCACGATGATAGTAAAAGGGAAAGATCAGCGAAGCCTCGATACAATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YIF1

Protein Analysis

209

Amino Acids

23.63

Weight (kDa)

5.99

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 34 - 190 4.2e-56 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 25
AccII CGCG 1 cut(s) 68
AciI CCGC 7 cut(s) 29, 60, 63, 66, 68, 90, 156
AclWI GGATC 2 cut(s) 305, 318
AgsI TTSAA 3 cut(s) 200, 241, 296
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 2 cut(s) 103, 576
AluI AGCT 2 cut(s) 103, 576
Alw26I GTCTC 1 cut(s) 71
AlwI GGATC 2 cut(s) 305, 318
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 2 cut(s) 92, 360
ApaI GGGCCC 1 cut(s) 96
ApeKI GCWGC 7 cut(s) 17, 71, 87, 161, 351, 497, 509
AspLEI GCGC 2 cut(s) 161, 459
AspS9I GGNCC 3 cut(s) 92, 93, 117
AsuHPI GGTGA 1 cut(s) 437
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 96
BamHI GGATCC 1 cut(s) 310
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 2 cut(s) 96, 173
BarI GAAGNNNNNNTAC 1 cut(s) 602
BbvI GCAGC 7 cut(s) 29, 83, 99, 148, 363, 484, 496
BccI CCATC 1 cut(s) 388
BceAI ACGGC 1 cut(s) 16
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 2 cut(s) 383, 489
BfoI RGCGCY 1 cut(s) 162
BglI GCCNNNNNGGC 1 cut(s) 68
Bme1390I CCNGG 1 cut(s) 209
Bme18I GGWCC 1 cut(s) 117
BmeT110I CYCGRG 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 3 cut(s) 92, 93, 117
BmiI GGNNCC 5 cut(s) 27, 94, 95, 172, 312
BmrFI CCNGG 1 cut(s) 209
Bsa29I ATCGAT 1 cut(s) 549
BsaJI CCNNGG 1 cut(s) 66
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bse118I RCCGGY 1 cut(s) 22
BseBI CCWGG 1 cut(s) 209
BseCI ATCGAT 1 cut(s) 549
BseDI CCNNGG 1 cut(s) 66
BseMII CTCAG 1 cut(s) 258
BseRI GAGGAG 1 cut(s) 163
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 7 cut(s) 29, 83, 99, 148, 363, 484, 496
BseYI CCCAGC 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 2 cut(s) 94, 362
BshNI GGYRCC 1 cut(s) 25
BshVI ATCGAT 1 cut(s) 549
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 23
BsmAI GTCTC 1 cut(s) 71
BsmBI CGTCTC 1 cut(s) 71
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 2 cut(s) 94, 362
BsoBI CYCGRG 1 cut(s) 104
Bsp120I GGGCCC 1 cut(s) 92
Bsp1286I GDGCHC 2 cut(s) 96, 173
Bsp143I GATC 4 cut(s) 229, 310, 564, 602
BspACI CCGC 7 cut(s) 29, 60, 63, 66, 68, 90, 156
BspANI GGCC 2 cut(s) 94, 362
BspCNI CTCAG 1 cut(s) 259
BspDI ATCGAT 1 cut(s) 549
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 5 cut(s) 27, 94, 95, 172, 312
BspPI GGATC 2 cut(s) 305, 318
BspQI GCTCTTC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 25
BsrFI RCCGGY 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 4 cut(s) 229, 310, 564, 602
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 2 cut(s) 205, 343
Bst6I CTCTTC 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 92
BstDEI CTNAG 2 cut(s) 141, 267
BstDSI CCRYGG 1 cut(s) 66
BstFNI CGCG 1 cut(s) 68
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 2 cut(s) 161, 459
BstKTI GATC 4 cut(s) 232, 313, 567, 605
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 4 cut(s) 229, 310, 564, 602
BstMWI GCNNNNNNNGC 6 cut(s) 23, 68, 71, 100, 463, 506
BstNI CCWGG 1 cut(s) 209
BstSCI CCNGG 1 cut(s) 207
BstSLI GKGCMC 1 cut(s) 96
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 7 cut(s) 29, 83, 99, 148, 363, 484, 496
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
Bsu15I ATCGAT 1 cut(s) 549
BsuRI GGCC 2 cut(s) 94, 362
BsuTUI ATCGAT 1 cut(s) 549
BtgI CCRYGG 1 cut(s) 66
BtrI CACGTC 1 cut(s) 43
BtsIMutI CAGTG 1 cut(s) 398
Cac8I GCNNGC 1 cut(s) 92
CfoI GCGC 2 cut(s) 161, 459
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 3 cut(s) 92, 93, 117
Cfr42I CCGCGG 1 cut(s) 69
ClaI ATCGAT 1 cut(s) 549
CviAII CATG 1 cut(s) 376
DdeI CTNAG 2 cut(s) 141, 267
DpnI GATC 4 cut(s) 231, 312, 566, 604
DpnII GATC 4 cut(s) 229, 310, 564, 602
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Eco24I GRGCYC 2 cut(s) 96, 173
Eco47I GGWCC 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 93
EcoRII CCWGG 1 cut(s) 207
EcoT38I GRGCYC 2 cut(s) 96, 173
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 1 cut(s) 379
FaiI YATR 2 cut(s) 228, 377
FalI AAGNNNNNCTT 4 cut(s) 104, 136, 459, 491
FatI CATG 1 cut(s) 375
FauI CCCGC 1 cut(s) 83
FbaI TGATCA 1 cut(s) 229
FriOI GRGCYC 2 cut(s) 96, 173
FspBI CTAG 2 cut(s) 383, 489
GlaI GCGC 2 cut(s) 160, 458
GsaI CCCAGC 1 cut(s) 291
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 2 cut(s) 94, 362
HapII CCGG 1 cut(s) 23
HhaI GCGC 2 cut(s) 161, 459
Hin1II CATG 1 cut(s) 379
Hin6I GCGC 2 cut(s) 159, 457
HinP1I GCGC 2 cut(s) 159, 457
HpaII CCGG 1 cut(s) 23
HphI GGTGA 1 cut(s) 437
Hpy166II GTNNAC 3 cut(s) 117, 339, 486
Hpy188I TCNGA 2 cut(s) 40, 234
Hpy188III TCNNGA 2 cut(s) 106, 314
Hpy8I GTNNAC 3 cut(s) 117, 339, 486
Hpy99I CGWCG 2 cut(s) 10, 44
HpyAV CCTTC 3 cut(s) 130, 352, 532
HpyCH4III ACNGT 2 cut(s) 205, 343
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 2 cut(s) 479, 500
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 68, 71, 100, 463, 506
HpyF3I CTNAG 2 cut(s) 141, 267
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 1 cut(s) 379
HspAI GCGC 2 cut(s) 159, 457
Ksp22I TGATCA 1 cut(s) 229
KspI CCGCGG 1 cut(s) 69
Kzo9I GATC 4 cut(s) 229, 310, 564, 602
LguI GCTCTTC 1 cut(s) 77
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 7 cut(s) 29, 83, 99, 148, 363, 484, 496
MaeI CTAG 2 cut(s) 383, 489
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 2 cut(s) 343, 553
MalI GATC 4 cut(s) 231, 312, 566, 604
MboI GATC 4 cut(s) 229, 310, 564, 602
MboII GAAGA 1 cut(s) 94
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 2 cut(s) 96, 173
MluCI AATT 1 cut(s) 413
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 9 cut(s) 40, 184, 301, 323, 415, 456, 499, 522, 624
MspA1I CMGCKG 2 cut(s) 68, 90
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 209
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 1 cut(s) 209
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 6 cut(s) 23, 68, 71, 100, 463, 506
NdeII GATC 4 cut(s) 229, 310, 564, 602
NlaIII CATG 1 cut(s) 379
NlaIV GGNNCC 5 cut(s) 27, 94, 95, 172, 312
NmeAIII GCCGAG 1 cut(s) 270
NmuCI GTSAC 1 cut(s) 343
PaeR7I CTCGAG 1 cut(s) 104
PciSI GCTCTTC 1 cut(s) 77
PctI GAATGC 1 cut(s) 499
PfoI TCCNGGA 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 207
PspFI CCCAGC 1 cut(s) 287
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 5 cut(s) 27, 94, 95, 172, 312
PspOMI GGGCCC 1 cut(s) 92
PspPI GGNCC 3 cut(s) 92, 93, 117
PsuI RGATCY 1 cut(s) 310
SacII CCGCGG 1 cut(s) 69
SapI GCTCTTC 1 cut(s) 77
Sau3AI GATC 4 cut(s) 229, 310, 564, 602
Sau96I GGNCC 3 cut(s) 92, 93, 117
ScrFI CCNGG 1 cut(s) 209
SduI GDGCHC 2 cut(s) 96, 173
SetI ASST 6 cut(s) 45, 105, 122, 197, 369, 578
Sfr274I CTCGAG 1 cut(s) 104
Sfr303I CCGCGG 1 cut(s) 69
SgrBI CCGCGG 1 cut(s) 69
SinI GGWCC 1 cut(s) 117
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 1 cut(s) 413
SsiI CCGC 7 cut(s) 29, 60, 63, 66, 68, 90, 156
SspMI CTAG 2 cut(s) 383, 489
StyD4I CCNGG 1 cut(s) 207
TaaI ACNGT 2 cut(s) 205, 343
TaiI ACGT 1 cut(s) 45
TaqI TCGA 4 cut(s) 105, 129, 549, 616
TasI AATT 1 cut(s) 413
TauI GCSGC 4 cut(s) 65, 68, 71, 159
TscAI CASTG 1 cut(s) 405
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 7 cut(s) 17, 71, 87, 161, 351, 497, 509
Tsp45I GTSAC 1 cut(s) 343
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 1 cut(s) 405
VpaK11BI GGWCC 1 cut(s) 117
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XhoI CTCGAG 1 cut(s) 104
XspI CTAG 2 cut(s) 383, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.