RLG00000008769

YIF1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009482
Physical Location & Seq
Forward (+)
36846044 .. 36853992
7949 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008769

Sequence Viewer

Length: 1197 bp
ATGAAGAAAGTAGTAAGAGACCATTCCGATGAAAGTGATGAAAGTAGAGATCATTCTAATGAAAGTGATGGAAATGGTTATAAATTATCTAGAGGCACTAGTGAAAAATCAATTGATAATGATGATGATGATGATGATGGTGATGATGGTGATGGTGGTAATATTAGTGGTGATAGCAACCATTGTTGCTATGGTGGTAGTGGACGTGATAACTCGTGTGACAATAGTAGTCATGACTACACTAATCTTGAGAAAGATGAGAATTGGATGATCGGAAGGTTGAACTGTGTTGCTAAGATCTCTTCGCCGGAGTTCTTTTCTCCTGAATTAACGGGAGAGAAGACTGAAGCTCCGCGTCGAGTCTTCACTTTTCCCTTGCCCATCGAAATGTACAATAATGTGGGAGCCCAGCCTGGTGTGCCGAGACCACCTGCAAATCCCCAGCCTAATCCGTTTGGAAATACGTTTTATGGTGCTGGTTCAGGACTTATCCGAGGTGGATTGGGTGCCTATGGAGAGAAAATTCTAGGATCAAGCTCTGAGTATGTACAAAGCAACATTAGTAGATATTTCTCTGATCCTCAGTATTACTTCCAAGTGAATGATCAGTATGTGAGGAACAAATTGAAGGTGGTTCTATTTCCATTTCTGCACAGGGGTCATTGGACACGGATAACTGAGCCCGTAGGAGGCAGACTTTCTTATAAACCCCCAATATACGATATCAATGCACCCGACCTATACATTCCCTTTATGGCATTTGGTACCTATGTGGTTTTGGCTGGCTTCTCATTGGGTCGTCATGGGAAGTTCAGCCCTGAGGCTCTAAACTGGTTATTTATCAAGGGATTGCTTGGCTGGTTTGTGCAAGTTATAATTCTGAAAGTCACGTTGTTTTCATTGGGTGGTGGTGAGGCGCCCTTGCTAGATATGGTGGCATATGCAGGGTATACTTTCACTGGGTTATGCTTGGCTGTGCTTGGACGAATTATGTGGAATTATTCATACTATTTTTTGATGCCTTGGACCTGTTTATGCATGGGAGTCTTCTTGGTGAAGACTATGAAGAGAGTCCTCTTTGCAGAGGTGAGGAGTTATGATTCAAGCAAGCACCATTATCTCCTGCTCTTCATTGCTTTGGCTCAGTTCCCAGTTTTTACTTGGCTTGGTAACATTAGTGTTAATTGGCTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YIF1

Protein Analysis

399

Amino Acids

44.58

Weight (kDa)

5.62

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YIF1 PF03878 170 - 389 7.6e-56 YIF1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 81, 705, 875
AarI CACCTGC 1 cut(s) 439
Acc36I ACCTGC 1 cut(s) 439
Acc65I GGTACC 1 cut(s) 764
AccB1I GGYRCC 3 cut(s) 506, 764, 916
AccI GTMKAC 1 cut(s) 950
AccII CGCG 1 cut(s) 355
AciI CCGC 1 cut(s) 353
AclWI GGATC 2 cut(s) 538, 572
AcsI RAATTY 1 cut(s) 522
AcuI CTGAAG 1 cut(s) 366
AcyI GRCGYC 1 cut(s) 917
AfaI GTAC 3 cut(s) 392, 549, 766
AfiI CCNNNNNNNGG 1 cut(s) 689
AgsI TTSAA 3 cut(s) 283, 628, 1104
AhlI ACTAGT 1 cut(s) 98
AjiI CACGTC 1 cut(s) 206
AjnI CCWGG 1 cut(s) 412
AluBI AGCT 2 cut(s) 350, 537
AluI AGCT 2 cut(s) 350, 537
Alw26I GTCTC 2 cut(s) 12, 418
AlwI GGATC 2 cut(s) 538, 572
ApoI RAATTY 1 cut(s) 522
Asp718I GGTACC 1 cut(s) 764
AspLEI GCGC 1 cut(s) 919
AspS9I GGNCC 1 cut(s) 1026
AsuHPI GGTGA 6 cut(s) 152, 161, 182, 923, 1066, 1099
AvaII GGWCC 1 cut(s) 1026
AxyI CCTNAGG 1 cut(s) 819
BaeI ACNNNNGTAYC 2 cut(s) 756, 789
BanI GGYRCC 3 cut(s) 506, 764, 916
BanII GRGCYC 2 cut(s) 409, 684
BauI CACGAG 1 cut(s) 214
BbsI GAAGAC 4 cut(s) 347, 355, 1039, 1064
BccI CCATC 5 cut(s) 62, 131, 140, 146, 389
BcgI CGANNNNNNTGC 2 cut(s) 710, 744
BciT130I CCWGG 1 cut(s) 414
BclI TGATCA 1 cut(s) 604
BcoDI GTCTC 2 cut(s) 12, 418
BcuI ACTAGT 1 cut(s) 98
BfaI CTAG 4 cut(s) 90, 99, 527, 926
BfoI RGCGCY 1 cut(s) 920
BfuAI ACCTGC 1 cut(s) 439
BglII AGATCT 1 cut(s) 297
Bme1390I CCNGG 1 cut(s) 414
Bme18I GGWCC 1 cut(s) 1026
BmgBI CACGTC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 1026
BmiI GGNNCC 4 cut(s) 406, 508, 766, 918
BmrFI CCNGG 1 cut(s) 414
BmrI ACTGGG 2 cut(s) 969, 1145
BmsI GCATC 1 cut(s) 1008
BmuI ACTGGG 2 cut(s) 969, 1145
BpiI GAAGAC 4 cut(s) 347, 355, 1039, 1064
BpuEI CTTGAG 1 cut(s) 269
BsaHI GRCGYC 1 cut(s) 917
BsaI GGTCTC 2 cut(s) 12, 418
BsaJI CCNNGG 2 cut(s) 493, 1022
BsaXI ACNNNNNCTCC 2 cut(s) 334, 364
Bsc4I CCNNNNNNNGG 1 cut(s) 689
Bse1I ACTGG 3 cut(s) 836, 964, 1151
Bse21I CCTNAGG 1 cut(s) 819
Bse3DI GCAATG 1 cut(s) 1131
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 2 cut(s) 493, 1022
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 689
BseMI GCAATG 1 cut(s) 1131
BseMII CTCAG 5 cut(s) 531, 596, 669, 810, 1157
BseNI ACTGG 3 cut(s) 836, 964, 1151
BseRI GAGGAG 1 cut(s) 1105
BseYI CCCAGC 2 cut(s) 408, 441
BsgI GTGCAG 1 cut(s) 635
Bsh1236I CGCG 1 cut(s) 355
BshNI GGYRCC 3 cut(s) 506, 764, 916
BsiSI CCGG 1 cut(s) 308
BslI CCNNNNNNNGG 1 cut(s) 689
BsmAI GTCTC 2 cut(s) 12, 418
Bso31I GGTCTC 2 cut(s) 12, 418
Bsp1286I GDGCHC 2 cut(s) 409, 684
Bsp1407I TGTACA 2 cut(s) 390, 547
Bsp143I GATC 6 cut(s) 49, 270, 297, 530, 577, 604
BspACI CCGC 1 cut(s) 353
BspCNI CTCAG 5 cut(s) 532, 595, 670, 811, 1156
BspFNI CGCG 1 cut(s) 355
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 4 cut(s) 406, 508, 766, 918
BspMI ACCTGC 1 cut(s) 439
BspPI GGATC 2 cut(s) 538, 572
BspQI GCTCTTC 1 cut(s) 1133
BspT107I GGYRCC 3 cut(s) 506, 764, 916
BspTNI GGTCTC 2 cut(s) 12, 418
BsrDI GCAATG 1 cut(s) 1131
BsrGI TGTACA 2 cut(s) 390, 547
BsrI ACTGG 3 cut(s) 836, 964, 1151
BssECI CCNNGG 2 cut(s) 493, 1022
BssMI GATC 6 cut(s) 49, 270, 297, 530, 577, 604
BssNAI GTATAC 1 cut(s) 951
BssNI GRCGYC 1 cut(s) 917
BssSI CACGAG 1 cut(s) 214
BssT1I CCWWGG 1 cut(s) 1022
Bst1107I GTATAC 1 cut(s) 951
Bst2BI CACGAG 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 1 cut(s) 287
Bst6I CTCTTC 3 cut(s) 307, 1061, 1133
BstACI GRCGYC 1 cut(s) 917
BstAUI TGTACA 2 cut(s) 390, 547
BstC8I GCNNGC 2 cut(s) 784, 1109
BstDEI CTNAG 6 cut(s) 294, 540, 582, 678, 819, 1143
BstF5I GGATG 1 cut(s) 273
BstFNI CGCG 1 cut(s) 355
BstH2I RGCGCY 1 cut(s) 920
BstHHI GCGC 1 cut(s) 919
BstKTI GATC 6 cut(s) 52, 273, 300, 533, 580, 607
BstMAI GTCTC 2 cut(s) 12, 418
BstMBI GATC 6 cut(s) 49, 270, 297, 530, 577, 604
BstMWI GCNNNNNNNGC 1 cut(s) 418
BstNI CCWGG 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 412
BstUI CGCG 1 cut(s) 355
BstV2I GAAGAC 4 cut(s) 347, 355, 1039, 1064
BstX2I RGATCY 1 cut(s) 297
BstYI RGATCY 1 cut(s) 297
BstZ17I GTATAC 1 cut(s) 951
Bsu36I CCTNAGG 1 cut(s) 819
BtrI CACGTC 1 cut(s) 206
BtsCI GGATG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 957
BveI ACCTGC 1 cut(s) 439
Cac8I GCNNGC 2 cut(s) 784, 1109
CciI TCATGA 1 cut(s) 232
CfoI GCGC 1 cut(s) 919
Cfr13I GGNCC 1 cut(s) 1026
CseI GACGC 1 cut(s) 344
Csp6I GTAC 3 cut(s) 391, 548, 765
CviAII CATG 3 cut(s) 233, 803, 1039
CviQI GTAC 3 cut(s) 391, 548, 765
DdeI CTNAG 6 cut(s) 294, 540, 582, 678, 819, 1143
DinI GGCGCC 1 cut(s) 918
DpnI GATC 6 cut(s) 51, 272, 299, 532, 579, 606
DpnII GATC 6 cut(s) 49, 270, 297, 530, 577, 604
Eam1104I CTCTTC 3 cut(s) 307, 1061, 1133
EarI CTCTTC 3 cut(s) 307, 1061, 1133
Eco130I CCWWGG 1 cut(s) 1022
Eco24I GRGCYC 2 cut(s) 409, 684
Eco31I GGTCTC 2 cut(s) 12, 418
Eco32I GATATC 1 cut(s) 724
Eco47I GGWCC 1 cut(s) 1026
Eco57I CTGAAG 1 cut(s) 366
Eco81I CCTNAGG 1 cut(s) 819
EcoRII CCWGG 1 cut(s) 412
EcoRV GATATC 1 cut(s) 724
EcoT14I CCWWGG 1 cut(s) 1022
EcoT22I ATGCAT 1 cut(s) 1040
EcoT38I GRGCYC 2 cut(s) 409, 684
EgeI GGCGCC 1 cut(s) 918
EheI GGCGCC 1 cut(s) 918
ErhI CCWWGG 1 cut(s) 1022
FaeI CATG 3 cut(s) 236, 806, 1042
FatI CATG 3 cut(s) 232, 802, 1038
FauNDI CATATG 1 cut(s) 940
FbaI TGATCA 1 cut(s) 604
FblI GTMKAC 1 cut(s) 950
FokI GGATG 1 cut(s) 280
FriOI GRGCYC 2 cut(s) 409, 684
FspBI CTAG 4 cut(s) 90, 99, 527, 926
GlaI GCGC 1 cut(s) 918
GsaI CCCAGC 2 cut(s) 412, 445
HaeII RGCGCY 1 cut(s) 920
HapII CCGG 1 cut(s) 308
HgaI GACGC 1 cut(s) 344
HhaI GCGC 1 cut(s) 919
Hin1I GRCGYC 1 cut(s) 917
Hin1II CATG 3 cut(s) 236, 806, 1042
Hin6I GCGC 1 cut(s) 917
HinP1I GCGC 1 cut(s) 917
HinfI GANTC 4 cut(s) 360, 1044, 1071, 1100
HpaII CCGG 1 cut(s) 308
HphI GGTGA 6 cut(s) 152, 161, 182, 923, 1066, 1099
Hpy166II GTNNAC 2 cut(s) 203, 951
Hpy188I TCNGA 6 cut(s) 28, 275, 494, 541, 577, 882
Hpy188III TCNNGA 5 cut(s) 90, 233, 248, 323, 483
Hpy8I GTNNAC 2 cut(s) 203, 951
Hpy99I CGWCG 1 cut(s) 360
HpyAV CCTTC 2 cut(s) 270, 622
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4IV ACGT 3 cut(s) 205, 464, 890
HpyCH4V TGCA 7 cut(s) 434, 652, 731, 868, 944, 1038, 1082
HpyF10VI GCNNNNNNNGC 1 cut(s) 418
HpyF3I CTNAG 6 cut(s) 294, 540, 582, 678, 819, 1143
HpySE526I ACGT 3 cut(s) 205, 464, 890
Hsp92I GRCGYC 1 cut(s) 917
Hsp92II CATG 3 cut(s) 236, 806, 1042
HspAI GCGC 1 cut(s) 917
KasI GGCGCC 1 cut(s) 916
KpnI GGTACC 1 cut(s) 768
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 6 cut(s) 49, 270, 297, 530, 577, 604
LguI GCTCTTC 1 cut(s) 1133
LmnI GCTCC 2 cut(s) 355, 404
LweI GCATC 1 cut(s) 1008
MaeI CTAG 4 cut(s) 90, 99, 527, 926
MaeII ACGT 3 cut(s) 205, 464, 890
MaeIII GTNAC 3 cut(s) 218, 886, 1169
MalI GATC 6 cut(s) 51, 272, 299, 532, 579, 606
MboI GATC 6 cut(s) 49, 270, 297, 530, 577, 604
MboII GAAGA 8 cut(s) 16, 294, 352, 355, 1039, 1069, 1078, 1120
MfeI CAATTG 1 cut(s) 111
MflI RGATCY 1 cut(s) 297
MhlI GDGCHC 2 cut(s) 409, 684
Mly113I GGCGCC 1 cut(s) 917
MlyI GAGTC 3 cut(s) 369, 1053, 1080
Mph1103I ATGCAT 1 cut(s) 1040
MseI TTAA 2 cut(s) 329, 1182
MslI CAYNNNNRTG 3 cut(s) 27, 57, 386
MspI CCGG 1 cut(s) 308
MspR9I CCNGG 1 cut(s) 414
MunI CAATTG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 414
MvnI CGCG 1 cut(s) 355
MwoI GCNNNNNNNGC 1 cut(s) 418
NarI GGCGCC 1 cut(s) 917
NdeI CATATG 1 cut(s) 940
NdeII GATC 6 cut(s) 49, 270, 297, 530, 577, 604
NlaIII CATG 3 cut(s) 236, 806, 1042
NlaIV GGNNCC 4 cut(s) 406, 508, 766, 918
NmeAIII GCCGAG 1 cut(s) 447
NmuCI GTSAC 2 cut(s) 218, 886
NsiI ATGCAT 1 cut(s) 1040
PagI TCATGA 1 cut(s) 232
PaqCI CACCTGC 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 1133
PfeI GAWTC 1 cut(s) 1100
PleI GAGTC 3 cut(s) 368, 1052, 1079
PluTI GGCGCC 1 cut(s) 920
PpsI GAGTC 3 cut(s) 368, 1052, 1079
PsiI TTATAA 3 cut(s) 81, 705, 875
Psp6I CCWGG 1 cut(s) 412
PspFI CCCAGC 2 cut(s) 408, 441
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 4 cut(s) 406, 508, 766, 918
PspPI GGNCC 1 cut(s) 1026
PsuI RGATCY 1 cut(s) 297
RsaI GTAC 3 cut(s) 392, 549, 766
RsaNI GTAC 3 cut(s) 391, 548, 765
RseI CAYNNNNRTG 3 cut(s) 27, 57, 386
SapI GCTCTTC 1 cut(s) 1133
SaqAI TTAA 2 cut(s) 329, 1182
Sau3AI GATC 6 cut(s) 49, 270, 297, 530, 577, 604
Sau96I GGNCC 1 cut(s) 1026
SchI GAGTC 3 cut(s) 369, 1053, 1080
ScrFI CCNGG 1 cut(s) 414
SduI GDGCHC 2 cut(s) 409, 684
SfaNI GCATC 1 cut(s) 1008
SfoI GGCGCC 1 cut(s) 918
SinI GGWCC 1 cut(s) 1026
SmiMI CAYNNNNRTG 3 cut(s) 27, 57, 386
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
SpeI ACTAGT 1 cut(s) 98
SsiI CCGC 1 cut(s) 353
SspDI GGCGCC 1 cut(s) 916
SspI AATATT 1 cut(s) 163
SspMI CTAG 4 cut(s) 90, 99, 527, 926
StyD4I CCNGG 1 cut(s) 412
StyI CCWWGG 1 cut(s) 1022
TaaI ACNGT 1 cut(s) 287
TaiI ACGT 3 cut(s) 208, 467, 893
TaqI TCGA 2 cut(s) 358, 384
TatI WGTACW 2 cut(s) 390, 547
TfiI GAWTC 1 cut(s) 1100
Tru1I TTAA 2 cut(s) 329, 1182
Tru9I TTAA 2 cut(s) 329, 1182
TscAI CASTG 1 cut(s) 964
TseFI GTSAC 2 cut(s) 218, 886
Tsp45I GTSAC 2 cut(s) 218, 886
TspDTI ATGAA 8 cut(s) 17, 45, 54, 75, 888, 993, 1079, 1120
TspGWI ACGGA 2 cut(s) 441, 685
TspRI CASTG 1 cut(s) 964
VpaK11BI GGWCC 1 cut(s) 1026
XapI RAATTY 1 cut(s) 522
XbaI TCTAGA 1 cut(s) 89
XcmI CCANNNNNNNNNTGG 2 cut(s) 188, 1158
XmiI GTMKAC 1 cut(s) 950
XspI CTAG 4 cut(s) 90, 99, 527, 926
Zsp2I ATGCAT 1 cut(s) 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.