Rw4G011300

YIF1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009482
Physical Location & Seq
Reverse (-)
26797031 .. 26801492
4462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011300.1

Sequence Viewer

Length: 810 bp
ATGTACAATAATGTGGGAGCCCAGCCTGGTGTGCCGAGACCACCTGCAAATCCCCAGCCTAATCCGTTTGGAAATACGTTTTATGGTGCTGGTTCAGGACTTATCCGAGGTGGATTGGGTGCCTATGGAGAGAAAATTCTAGGATCAAGCTCTGAGTATGTACAAAGCAACATTAGTAGATATTTCTCTGATCCTCAGTATTACTTCCAAGTGAATGATCAGTATGTGAGGAACAAATTGAAGGTGGTTCTATTTCCATTTCTGCACAGGGGTCATTGGACACGGATAACTGAGCCCGTAGGAGGCAGACTTTCTTATAAACCCCCAATATACGATATCAATGCACCCGACCTATACATTCCCTTTATGGCATTTGGTACCTATGTGGTTTTGGCTGGCTTCTCATTGGGTCGTCATGGGAAGTTCAGCCCTGAGGCTCTAAACTGGTTATTTATCAAGGGATTGCTTGGCTGGTTTGTGCAAGTTATAATTCTGAAAATCACGTTGTTTTCATTGGGTGGTGGTGAGGCGCCCTTGCTAGATATGGTGGCATATGCAGGGTATACTTTCACTGGGTTATGCTTGGCTGTGCTTGGACGGATTATGTGGAATTATTCGTACTATTTTTTGATGCCTTGGACCTGTTTATGCATGGGAGTCTTCTTGGTGAAGACTATGAAGAGAGTCCTCTTTGCAGAGGTGAGGAGTTATGATTCAAGCAAGCACCATTATCTCCTGCTCTTCATTGCTTTGGCTCAGTTCCCAGTTTTTACTTGGCTTGGTAACATTAGTGTTAATTGGCTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YIF1

Protein Analysis

269

Amino Acids

30.37

Weight (kDa)

9.4

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YIF1 PF03878 41 - 260 2e-56 YIF1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 318, 488
AarI CACCTGC 1 cut(s) 52
Acc36I ACCTGC 1 cut(s) 52
Acc65I GGTACC 1 cut(s) 377
AccB1I GGYRCC 3 cut(s) 119, 377, 529
AccI GTMKAC 1 cut(s) 563
AclWI GGATC 2 cut(s) 151, 185
AcsI RAATTY 1 cut(s) 135
AcyI GRCGYC 1 cut(s) 530
AfaI GTAC 4 cut(s) 5, 162, 379, 620
AfiI CCNNNNNNNGG 1 cut(s) 302
AgsI TTSAA 2 cut(s) 241, 717
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 1 cut(s) 150
AluI AGCT 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 31
AlwI GGATC 2 cut(s) 151, 185
ApoI RAATTY 1 cut(s) 135
Asp718I GGTACC 1 cut(s) 377
AspLEI GCGC 1 cut(s) 532
AspS9I GGNCC 1 cut(s) 639
AsuHPI GGTGA 3 cut(s) 536, 679, 712
AvaII GGWCC 1 cut(s) 639
AxyI CCTNAGG 1 cut(s) 432
BaeI ACNNNNGTAYC 2 cut(s) 369, 402
BanI GGYRCC 3 cut(s) 119, 377, 529
BanII GRGCYC 2 cut(s) 22, 297
BbsI GAAGAC 2 cut(s) 652, 677
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BciT130I CCWGG 1 cut(s) 27
BclI TGATCA 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 31
BfaI CTAG 2 cut(s) 140, 539
BfoI RGCGCY 1 cut(s) 533
BfuAI ACCTGC 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 1 cut(s) 639
BmgT120I GGNCC 1 cut(s) 639
BmiI GGNNCC 4 cut(s) 19, 121, 379, 531
BmrFI CCNGG 1 cut(s) 27
BmrI ACTGGG 2 cut(s) 582, 758
BmsI GCATC 1 cut(s) 621
BmuI ACTGGG 2 cut(s) 582, 758
BpiI GAAGAC 2 cut(s) 652, 677
BsaHI GRCGYC 1 cut(s) 530
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 2 cut(s) 106, 635
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse1I ACTGG 3 cut(s) 449, 577, 764
Bse21I CCTNAGG 1 cut(s) 432
Bse3DI GCAATG 1 cut(s) 744
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 2 cut(s) 106, 635
BseLI CCNNNNNNNGG 1 cut(s) 302
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 5 cut(s) 144, 209, 282, 423, 770
BseNI ACTGG 3 cut(s) 449, 577, 764
BseRI GAGGAG 1 cut(s) 718
BseYI CCCAGC 2 cut(s) 21, 54
BsgI GTGCAG 1 cut(s) 248
BshNI GGYRCC 3 cut(s) 119, 377, 529
BslI CCNNNNNNNGG 1 cut(s) 302
BsmAI GTCTC 1 cut(s) 31
Bso31I GGTCTC 1 cut(s) 31
Bsp1286I GDGCHC 2 cut(s) 22, 297
Bsp1407I TGTACA 2 cut(s) 3, 160
Bsp143I GATC 3 cut(s) 143, 190, 217
BspCNI CTCAG 5 cut(s) 145, 208, 283, 424, 769
BspLI GGNNCC 4 cut(s) 19, 121, 379, 531
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 2 cut(s) 151, 185
BspQI GCTCTTC 1 cut(s) 746
BspT107I GGYRCC 3 cut(s) 119, 377, 529
BspTNI GGTCTC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 744
BsrGI TGTACA 2 cut(s) 3, 160
BsrI ACTGG 3 cut(s) 449, 577, 764
BssECI CCNNGG 2 cut(s) 106, 635
BssMI GATC 3 cut(s) 143, 190, 217
BssNAI GTATAC 1 cut(s) 564
BssNI GRCGYC 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 635
Bst1107I GTATAC 1 cut(s) 564
Bst2UI CCWGG 1 cut(s) 27
Bst6I CTCTTC 2 cut(s) 674, 746
BstACI GRCGYC 1 cut(s) 530
BstAUI TGTACA 2 cut(s) 3, 160
BstC8I GCNNGC 2 cut(s) 397, 722
BstDEI CTNAG 5 cut(s) 153, 195, 291, 432, 756
BstH2I RGCGCY 1 cut(s) 533
BstHHI GCGC 1 cut(s) 532
BstKTI GATC 3 cut(s) 146, 193, 220
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 3 cut(s) 143, 190, 217
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstNI CCWGG 1 cut(s) 27
BstSCI CCNGG 1 cut(s) 25
BstV2I GAAGAC 2 cut(s) 652, 677
BstZ17I GTATAC 1 cut(s) 564
Bsu36I CCTNAGG 1 cut(s) 432
BtsIMutI CAGTG 1 cut(s) 570
BveI ACCTGC 1 cut(s) 52
Cac8I GCNNGC 2 cut(s) 397, 722
CfoI GCGC 1 cut(s) 532
Cfr13I GGNCC 1 cut(s) 639
Csp6I GTAC 4 cut(s) 4, 161, 378, 619
CviAII CATG 2 cut(s) 416, 652
CviQI GTAC 4 cut(s) 4, 161, 378, 619
DdeI CTNAG 5 cut(s) 153, 195, 291, 432, 756
DinI GGCGCC 1 cut(s) 531
DpnI GATC 3 cut(s) 145, 192, 219
DpnII GATC 3 cut(s) 143, 190, 217
Eam1104I CTCTTC 2 cut(s) 674, 746
EarI CTCTTC 2 cut(s) 674, 746
Eco130I CCWWGG 1 cut(s) 635
Eco24I GRGCYC 2 cut(s) 22, 297
Eco31I GGTCTC 1 cut(s) 31
Eco32I GATATC 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 639
Eco81I CCTNAGG 1 cut(s) 432
EcoRII CCWGG 1 cut(s) 25
EcoRV GATATC 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 635
EcoT22I ATGCAT 1 cut(s) 653
EcoT38I GRGCYC 2 cut(s) 22, 297
EgeI GGCGCC 1 cut(s) 531
EheI GGCGCC 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 635
FaeI CATG 2 cut(s) 419, 655
FatI CATG 2 cut(s) 415, 651
FauNDI CATATG 1 cut(s) 553
FbaI TGATCA 1 cut(s) 217
FblI GTMKAC 1 cut(s) 563
FriOI GRGCYC 2 cut(s) 22, 297
FspBI CTAG 2 cut(s) 140, 539
GlaI GCGC 1 cut(s) 531
GsaI CCCAGC 2 cut(s) 25, 58
HaeII RGCGCY 1 cut(s) 533
HhaI GCGC 1 cut(s) 532
Hin1I GRCGYC 1 cut(s) 530
Hin1II CATG 2 cut(s) 419, 655
Hin6I GCGC 1 cut(s) 530
HinP1I GCGC 1 cut(s) 530
HinfI GANTC 3 cut(s) 657, 684, 713
HphI GGTGA 3 cut(s) 536, 679, 712
Hpy166II GTNNAC 1 cut(s) 564
Hpy188I TCNGA 4 cut(s) 107, 154, 190, 495
Hpy188III TCNNGA 1 cut(s) 96
Hpy8I GTNNAC 1 cut(s) 564
HpyAV CCTTC 1 cut(s) 235
HpyCH4IV ACGT 2 cut(s) 77, 503
HpyCH4V TGCA 7 cut(s) 47, 265, 344, 481, 557, 651, 695
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 5 cut(s) 153, 195, 291, 432, 756
HpySE526I ACGT 2 cut(s) 77, 503
Hsp92I GRCGYC 1 cut(s) 530
Hsp92II CATG 2 cut(s) 419, 655
HspAI GCGC 1 cut(s) 530
KasI GGCGCC 1 cut(s) 529
KpnI GGTACC 1 cut(s) 381
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 3 cut(s) 143, 190, 217
LguI GCTCTTC 1 cut(s) 746
LmnI GCTCC 1 cut(s) 17
LweI GCATC 1 cut(s) 621
MaeI CTAG 2 cut(s) 140, 539
MaeII ACGT 2 cut(s) 77, 503
MaeIII GTNAC 1 cut(s) 782
MalI GATC 3 cut(s) 145, 192, 219
MboI GATC 3 cut(s) 143, 190, 217
MboII GAAGA 4 cut(s) 652, 682, 691, 733
MhlI GDGCHC 2 cut(s) 22, 297
MluCI AATT 5 cut(s) 135, 236, 489, 610, 796
Mly113I GGCGCC 1 cut(s) 530
MlyI GAGTC 2 cut(s) 666, 693
MnlI CCTC 9 cut(s) 101, 204, 222, 296, 427, 520, 691, 696, 698
Mph1103I ATGCAT 1 cut(s) 653
MseI TTAA 1 cut(s) 795
MspR9I CCNGG 1 cut(s) 27
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 31
NarI GGCGCC 1 cut(s) 530
NdeI CATATG 1 cut(s) 553
NdeII GATC 3 cut(s) 143, 190, 217
NlaIII CATG 2 cut(s) 419, 655
NlaIV GGNNCC 4 cut(s) 19, 121, 379, 531
NmeAIII GCCGAG 1 cut(s) 60
NsiI ATGCAT 1 cut(s) 653
PaqCI CACCTGC 1 cut(s) 52
PciSI GCTCTTC 1 cut(s) 746
PfeI GAWTC 1 cut(s) 713
PleI GAGTC 2 cut(s) 665, 692
PluTI GGCGCC 1 cut(s) 533
PpsI GAGTC 2 cut(s) 665, 692
PsiI TTATAA 2 cut(s) 318, 488
Psp6I CCWGG 1 cut(s) 25
PspFI CCCAGC 2 cut(s) 21, 54
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 4 cut(s) 19, 121, 379, 531
PspPI GGNCC 1 cut(s) 639
RsaI GTAC 4 cut(s) 5, 162, 379, 620
RsaNI GTAC 4 cut(s) 4, 161, 378, 619
SapI GCTCTTC 1 cut(s) 746
SaqAI TTAA 1 cut(s) 795
Sau3AI GATC 3 cut(s) 143, 190, 217
Sau96I GGNCC 1 cut(s) 639
SchI GAGTC 2 cut(s) 666, 693
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 2 cut(s) 22, 297
SfaNI GCATC 1 cut(s) 621
SfoI GGCGCC 1 cut(s) 531
SinI GGWCC 1 cut(s) 639
Sse9I AATT 5 cut(s) 135, 236, 489, 610, 796
SspDI GGCGCC 1 cut(s) 529
SspMI CTAG 2 cut(s) 140, 539
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 1 cut(s) 635
TaiI ACGT 2 cut(s) 80, 506
TasI AATT 5 cut(s) 135, 236, 489, 610, 796
TatI WGTACW 2 cut(s) 3, 160
TfiI GAWTC 1 cut(s) 713
Tru1I TTAA 1 cut(s) 795
Tru9I TTAA 1 cut(s) 795
TscAI CASTG 1 cut(s) 577
TspDTI ATGAA 3 cut(s) 501, 692, 733
TspGWI ACGGA 3 cut(s) 54, 298, 613
TspRI CASTG 1 cut(s) 577
VpaK11BI GGWCC 1 cut(s) 639
XapI RAATTY 1 cut(s) 135
XcmI CCANNNNNNNNNTGG 1 cut(s) 771
XmiI GTMKAC 1 cut(s) 563
XspI CTAG 2 cut(s) 140, 539
Zsp2I ATGCAT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.