Rh4DG133000

YIF1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009482
Physical Location & Seq
Reverse (-)
26530187 .. 26536278
6092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG133000.1

Sequence Viewer

Length: 684 bp
ATGGTGATTGAGCTTTATTCTGATAGCTTCTGTTTAAATGAGCAGATTAGTAGATATTTCTCTGATCCTCAGTATTACTTCCAAGTGAATGATCAGTATGTGAGGAACAAATTGAAGGTGGTTCTATTTCCATTTCTGCACAGGGGTCATTGGACACGGATAACTGAGCCCGTAGGAGGCAGACTTTCTTATAAACCCCCAATATACGATATCAATGCACCCGACCTATACATTCCCTTTATGGCATTTGGTACCTATGTGGTTTTGGCTGGCTTCTCATTGGGTCGTCATGGGAAGTTCAGCCCTGAGGCTCTAAACTGGTTATTTATCAAGGGATTGCTTGGCTGGTTTGTGCAAGTTATAATTCTGAAAATCACGTTGTTTTCATTGGGTGGTGGTGAGGCGCCCTTGCTAGATATGGTGGCATATGCAGGGTATACTTTCACTGGGTTATGCTTGGCTGTGCTTGGACGGATTATGTGGAATTATTCGTACTATTTTTTGATGCCTTGGACCTGTTTATGCATGGGAGTCTTCTTGGTGAAGACTATGAAGAGAGTCCTCTTTGCAGAGGTGAGGAGTTATGATTCAAGCAAGCACCATTATCTCCTGCTCTTCATTGCTTTGGCTCAGTTCCCAGTTTTTACTTGGCTTGGTAACATTAGTGTTAATTGGCTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YIF1

Protein Analysis

227

Amino Acids

26.3

Weight (kDa)

8.96

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YIF1 PF03878 12 - 218 1.3e-51 YIF1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 192, 362
Acc65I GGTACC 1 cut(s) 251
AccB1I GGYRCC 2 cut(s) 251, 403
AccI GTMKAC 1 cut(s) 437
AclWI GGATC 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 404
AfaI GTAC 2 cut(s) 253, 494
AfiI CCNNNNNNNGG 1 cut(s) 176
AgsI TTSAA 2 cut(s) 115, 591
AluBI AGCT 2 cut(s) 13, 27
AluI AGCT 2 cut(s) 13, 27
AlwI GGATC 1 cut(s) 59
Asp718I GGTACC 1 cut(s) 251
AspLEI GCGC 1 cut(s) 406
AspS9I GGNCC 1 cut(s) 513
AsuHPI GGTGA 4 cut(s) 16, 410, 553, 586
AvaII GGWCC 1 cut(s) 513
AxyI CCTNAGG 1 cut(s) 306
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BanI GGYRCC 2 cut(s) 251, 403
BanII GRGCYC 1 cut(s) 171
BbsI GAAGAC 2 cut(s) 526, 551
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BclI TGATCA 1 cut(s) 91
BfaI CTAG 1 cut(s) 413
BfoI RGCGCY 1 cut(s) 407
Bme18I GGWCC 1 cut(s) 513
BmgT120I GGNCC 1 cut(s) 513
BmiI GGNNCC 2 cut(s) 253, 405
BmrI ACTGGG 2 cut(s) 456, 632
BmsI GCATC 1 cut(s) 495
BmuI ACTGGG 2 cut(s) 456, 632
BpiI GAAGAC 2 cut(s) 526, 551
BsaHI GRCGYC 1 cut(s) 404
BsaJI CCNNGG 1 cut(s) 509
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse1I ACTGG 3 cut(s) 323, 451, 638
Bse21I CCTNAGG 1 cut(s) 306
Bse3DI GCAATG 1 cut(s) 618
BseDI CCNNGG 1 cut(s) 509
BseLI CCNNNNNNNGG 1 cut(s) 176
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 4 cut(s) 83, 156, 297, 644
BseNI ACTGG 3 cut(s) 323, 451, 638
BseRI GAGGAG 1 cut(s) 592
BsgI GTGCAG 1 cut(s) 122
BshNI GGYRCC 2 cut(s) 251, 403
BslI CCNNNNNNNGG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 2 cut(s) 64, 91
BspCNI CTCAG 4 cut(s) 82, 157, 298, 643
BspLI GGNNCC 2 cut(s) 253, 405
BspPI GGATC 1 cut(s) 59
BspQI GCTCTTC 1 cut(s) 620
BspT107I GGYRCC 2 cut(s) 251, 403
BsrDI GCAATG 1 cut(s) 618
BsrI ACTGG 3 cut(s) 323, 451, 638
BssECI CCNNGG 1 cut(s) 509
BssMI GATC 2 cut(s) 64, 91
BssNAI GTATAC 1 cut(s) 438
BssNI GRCGYC 1 cut(s) 404
BssT1I CCWWGG 1 cut(s) 509
Bst1107I GTATAC 1 cut(s) 438
Bst6I CTCTTC 2 cut(s) 548, 620
BstACI GRCGYC 1 cut(s) 404
BstC8I GCNNGC 2 cut(s) 271, 596
BstDEI CTNAG 4 cut(s) 69, 165, 306, 630
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 1 cut(s) 406
BstKTI GATC 2 cut(s) 67, 94
BstMBI GATC 2 cut(s) 64, 91
BstV2I GAAGAC 2 cut(s) 526, 551
BstZ17I GTATAC 1 cut(s) 438
Bsu36I CCTNAGG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 444
Cac8I GCNNGC 2 cut(s) 271, 596
CfoI GCGC 1 cut(s) 406
Cfr13I GGNCC 1 cut(s) 513
Csp6I GTAC 2 cut(s) 252, 493
CviAII CATG 2 cut(s) 290, 526
CviQI GTAC 2 cut(s) 252, 493
DdeI CTNAG 4 cut(s) 69, 165, 306, 630
DinI GGCGCC 1 cut(s) 405
DpnI GATC 2 cut(s) 66, 93
DpnII GATC 2 cut(s) 64, 91
DraI TTTAAA 1 cut(s) 36
Eam1104I CTCTTC 2 cut(s) 548, 620
EarI CTCTTC 2 cut(s) 548, 620
Eco130I CCWWGG 1 cut(s) 509
Eco24I GRGCYC 1 cut(s) 171
Eco32I GATATC 1 cut(s) 211
Eco47I GGWCC 1 cut(s) 513
Eco81I CCTNAGG 1 cut(s) 306
EcoRV GATATC 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 509
EcoT22I ATGCAT 1 cut(s) 527
EcoT38I GRGCYC 1 cut(s) 171
EgeI GGCGCC 1 cut(s) 405
EheI GGCGCC 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 509
FaeI CATG 2 cut(s) 293, 529
FatI CATG 2 cut(s) 289, 525
FauNDI CATATG 1 cut(s) 427
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 437
FriOI GRGCYC 1 cut(s) 171
FspBI CTAG 1 cut(s) 413
GlaI GCGC 1 cut(s) 405
HaeII RGCGCY 1 cut(s) 407
HhaI GCGC 1 cut(s) 406
Hin1I GRCGYC 1 cut(s) 404
Hin1II CATG 2 cut(s) 293, 529
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HinfI GANTC 3 cut(s) 531, 558, 587
HphI GGTGA 4 cut(s) 16, 410, 553, 586
Hpy166II GTNNAC 1 cut(s) 438
Hpy188I TCNGA 3 cut(s) 22, 64, 369
Hpy8I GTNNAC 1 cut(s) 438
HpyAV CCTTC 1 cut(s) 109
HpyCH4IV ACGT 1 cut(s) 377
HpyCH4V TGCA 6 cut(s) 139, 218, 355, 431, 525, 569
HpyF3I CTNAG 4 cut(s) 69, 165, 306, 630
HpySE526I ACGT 1 cut(s) 377
Hsp92I GRCGYC 1 cut(s) 404
Hsp92II CATG 2 cut(s) 293, 529
HspAI GCGC 1 cut(s) 404
KasI GGCGCC 1 cut(s) 403
KpnI GGTACC 1 cut(s) 255
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 2 cut(s) 64, 91
LguI GCTCTTC 1 cut(s) 620
LweI GCATC 1 cut(s) 495
MaeI CTAG 1 cut(s) 413
MaeII ACGT 1 cut(s) 377
MaeIII GTNAC 1 cut(s) 656
MalI GATC 2 cut(s) 66, 93
MboI GATC 2 cut(s) 64, 91
MboII GAAGA 4 cut(s) 526, 556, 565, 607
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 4 cut(s) 110, 363, 484, 670
Mly113I GGCGCC 1 cut(s) 404
MlyI GAGTC 2 cut(s) 540, 567
MnlI CCTC 8 cut(s) 78, 96, 170, 301, 394, 565, 570, 572
Mph1103I ATGCAT 1 cut(s) 527
MseI TTAA 2 cut(s) 35, 669
NarI GGCGCC 1 cut(s) 404
NdeI CATATG 1 cut(s) 427
NdeII GATC 2 cut(s) 64, 91
NlaIII CATG 2 cut(s) 293, 529
NlaIV GGNNCC 2 cut(s) 253, 405
NsiI ATGCAT 1 cut(s) 527
PciSI GCTCTTC 1 cut(s) 620
PfeI GAWTC 1 cut(s) 587
PleI GAGTC 2 cut(s) 539, 566
PluTI GGCGCC 1 cut(s) 407
PpsI GAGTC 2 cut(s) 539, 566
PsiI TTATAA 2 cut(s) 192, 362
PspN4I GGNNCC 2 cut(s) 253, 405
PspPI GGNCC 1 cut(s) 513
RsaI GTAC 2 cut(s) 253, 494
RsaNI GTAC 2 cut(s) 252, 493
SapI GCTCTTC 1 cut(s) 620
SaqAI TTAA 2 cut(s) 35, 669
Sau3AI GATC 2 cut(s) 64, 91
Sau96I GGNCC 1 cut(s) 513
SchI GAGTC 2 cut(s) 540, 567
SduI GDGCHC 1 cut(s) 171
SetI ASST 8 cut(s) 15, 29, 120, 228, 257, 380, 518, 576
SfaNI GCATC 1 cut(s) 495
SfoI GGCGCC 1 cut(s) 405
SinI GGWCC 1 cut(s) 513
Sse9I AATT 4 cut(s) 110, 363, 484, 670
SspDI GGCGCC 1 cut(s) 403
SspMI CTAG 1 cut(s) 413
StyI CCWWGG 1 cut(s) 509
TaiI ACGT 1 cut(s) 380
TasI AATT 4 cut(s) 110, 363, 484, 670
TfiI GAWTC 1 cut(s) 587
Tru1I TTAA 2 cut(s) 35, 669
Tru9I TTAA 2 cut(s) 35, 669
TscAI CASTG 1 cut(s) 451
TspDTI ATGAA 3 cut(s) 375, 566, 607
TspGWI ACGGA 2 cut(s) 172, 487
TspRI CASTG 1 cut(s) 451
VpaK11BI GGWCC 1 cut(s) 513
XcmI CCANNNNNNNNNTGG 1 cut(s) 645
XmiI GTMKAC 1 cut(s) 437
XspI CTAG 1 cut(s) 413
Zsp2I ATGCAT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.