Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012146

Physical Location & Seq

Genomic Coordinates Forward (+)

3031694 .. 3039440

Gene Structure 7747 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1024300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1866 bp

ATGGCGGATCACGAGGAGGAGGACCTCCGAATGGCACTGCGGATGAGTATGCAGAATTCACCTCCGGAGCCCAAGCGTAGCAAGCCCAGGGACGGACCCGCCGGAGCTCCGGCTGAGGAGCCGTCGGAAGTGAAGACCCGGAGACTTCAGCGGGAGCTCATGGCTGCTGCTGCTGAGAAGAGAATGCTGGACATCAGTAAGTGTCCGCCTTCGGCTTCGGCTTCACCTAGCAAGGTGTTGTCGGGGTCTGCGGGTTCCGGTTCGGGTTTGGCGCCAAACGTGGCGACGAAAGATAAGGAATTGAGCTTGGAGGAAGTGAATTTGGGAAAGGGGTTGAGCGAAGAGGATGCAAAGCAGTTGTTTTCGATGGTTTTCGGACCCGAGGTTACGAAGGGCATTCTTGTGCAGTGGAGTAATCAGGGAATAAGGTTTAGCTCTGATCCAGAAACATCTATGGGTCTAGTGCAGCATGAAGGTGGGCCCTGTGGCGTTTTAGCAGCCATTCAAGCATTTGTTCTCAAATACCTCCTTTTCTACCCAGCTGAATTAGGTAAAGTTGCACCAAACATGCATCAAAATACGGGTTCAAGGACACCGTCTAATAGTCAGTGTGTTGCATCGAATAATTTTGCCTCTCTTACTGAAGATGCAAAAGCAAGTGTACTTATTAGAAGTATGGGTGAGATATTGTTCTTATGTGGAAGCAATAAAAGGGCGGTGATTGCAACTTTGAGTGTTATTGGAGATGAAACTGAGAGGGCTGATGACAACCTGAATGATGAAGTAATAACAAAGTCACTTGAAAGTCTTTCAATCGAATCAGCTGCTGATTTGCATAAAGTGCTAAGAGTTAGCACTTACACAACACAAGAAAGTGCGTTTATGGGGCTCCAAGCAGTGCTTCCTGTCTTCCAAAGTCGTATGGGGGCACTGCTGTTCCTTATTTCTGCTTTACTTTCTCGAGGACTGGACTTGGTACAATCTGACAGGGATGACCCCGGCCTACCTCTTGTCACTGCTCCATTTGGGCATGCTTCACAGGAAATTGTGAACTTATTGCTCTGTGGGCAGGCAGTCCCTAATGTTTTCGATGGAAAGATGGATTTAGGTGGTGGCATGTCTTTAAAAGGCATATCCAAAACTGTGGAAGTTGGATTCCTCACTCTGTTAGAATCCCTAAATTACTGTAAGGTTGGTCAGTATTTGAAAAGTCCAAAATGGCCAATCTGGGTGGTTGGGAGTGAGTCTCATTATACAGTCCTTTTTTCTCTTGATACCACTGTTCAAGATGAGAATGAACTGGAAGGAAGGGAATCACAGATTCGGAGAGCTTTTGATGCACAAGATCAGAGTGGTGGTGGTGGTTTTATTAGTGTGGAAGGCTTTCATCAAGTCCTTAGGGAAACTAACGTAAAACTTCCAACTGAGAAGGTTGACCTCCTCTGCAACACAGGATTTATCGTATGGAGTGAGTTGTGGCAGGTCATTTTGGATTTGGACAAGAGCTTGGGAGGCCTGAAGGATTCAACTGGATTGATGGGTAAGAAGGTGTTTGATCTTTACCATTTTAATGGAATTGCAAAATCTGACCTGAATGGAAGCCAGACAGCCTCTGGAGGCGAGATTCCAGTTCAAAGACCTAGGCTCACAAAATTGAGGGTGTCGGTACCTCCAAGGTGGACTCCTGAAGAATACATGACAGATGTGGTAGTGTCCTCTGCTTCTAGTGGTAATGAATCTGGAGGGAAAGTCACAGAAATTGCTAAGCCGGAGCCTGCTCAGCATGCACCTCTGGTGGACTGCATAAGAACACGCTGGCCCCGTGCTGTCTGCAATTGGGTAGGTGACCCGCCTAGCATAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4205

Protein Analysis

622

Amino Acids

67.09

Weight (kDa)

5.28

Isoelectric Point (pI)

48.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MINDY-3_4_CD	PF13898	123 - 433	5.9e-60	Deubiquitinating enzyme MINDY-3/4, conserved domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012146)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G43690
fragaria_vesca	FvH4_2g13910 FvH4_2g13910
malus_domestica	MD05G1023700.v1.1 MD10G1024300.v1.1
prunus_persica	Prupe.8G028200_v2.0.a1 Prupe.8G028200_v2.0.a1
pyrus_communis	pycom05g01520 pycom10g01610
rosa_chinensis	RchiOBHm_Chr6g0275661
rosa_laevigata	RLG00000013449
rosa_multiflora	Rmu_sc0011044.1_g000001
rosa_roxburghii	Rroxscaffold_7G00193050
rosa_rugosa	Rorug06G0095400
rosa_samantha	Rh6AG207300 Rh6BG209900 Rh6CG213300 Rh6DG203000
rosa_wichuraiana	Rw6G018070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1471
Acc65I	GGTACC	1 cut(s)	1666
AccB1I	GGYRCC	2 cut(s)	271, 1666
AccIII	TCCGGA	1 cut(s)	64
AciI	CCGC	8 cut(s)	5, 40, 99, 151, 206, 251, 716, 1850
AclWI	GGATC	2 cut(s)	15, 434
AcoI	YGGCCR	1 cut(s)	1220
AcsI	RAATTY	2 cut(s)	55, 319
AcuI	CTGAAG	4 cut(s)	131, 663, 1538, 1707
AcyI	GRCGYC	1 cut(s)	272
AfaI	GTAC	3 cut(s)	663, 978, 1668
AfiI	CCNNNNNNNGG	2 cut(s)	31, 92
AgsI	TTSAA	8 cut(s)	506, 588, 803, 813, 1207, 1286, 1527, 1634
AjnI	CCWGG	1 cut(s)	86
AjuI	GAANNNNNNNTTGG	2 cut(s)	885, 917
AluBI	AGCT	8 cut(s)	107, 157, 306, 435, 542, 824, 1331, 1506
AluI	AGCT	8 cut(s)	107, 157, 306, 435, 542, 824, 1331, 1506
Alw21I	GWGCWC	2 cut(s)	109, 159
Alw26I	GTCTC	2 cut(s)	136, 1251
AlwI	GGATC	2 cut(s)	15, 434
AlwNI	CAGNNNCTG	2 cut(s)	827, 1613
Ama87I	CYCGRG	2 cut(s)	380, 960
Aor13HI	TCCGGA	1 cut(s)	64
AoxI	GGCC	5 cut(s)	479, 1000, 1220, 1513, 1817
ApaI	GGGCCC	1 cut(s)	483
ApeKI	GCWGC	6 cut(s)	164, 167, 170, 466, 497, 824
ApoI	RAATTY	2 cut(s)	55, 319
Asp700I	GAANNNNTTC	1 cut(s)	1383
Asp718I	GGTACC	1 cut(s)	1666
AspA2I	CCTAGG	1 cut(s)	1640
AspLEI	GCGC	1 cut(s)	274
AspS9I	GGNCC	6 cut(s)	22, 95, 377, 479, 480, 1818
AsuC2I	CCSGG	2 cut(s)	139, 999
AsuHPI	GGTGA	5 cut(s)	51, 216, 692, 730, 1856
AvaI	CYCGRG	2 cut(s)	380, 960
AvaII	GGWCC	3 cut(s)	22, 95, 377
AvrII	CCTAGG	1 cut(s)	1640
AxyI	CCTNAGG	1 cut(s)	1397
BaeGI	GKGCMC	2 cut(s)	483, 931
BaeI	ACNNNNGTAYC	2 cut(s)	1266, 1299
BalI	TGGCCA	1 cut(s)	1222
BanI	GGYRCC	2 cut(s)	271, 1666
BanII	GRGCYC	5 cut(s)	72, 109, 159, 483, 891
BauI	CACGAG	1 cut(s)	11
BbsI	GAAGAC	2 cut(s)	140, 901
Bbv12I	GWGCWC	2 cut(s)	109, 159
BbvCI	CCTCAGC	1 cut(s)	114
BbvI	GCAGC	6 cut(s)	151, 154, 157, 478, 509, 811
BccI	CCATC	4 cut(s)	361, 1085, 1093, 1531
BceAI	ACGGC	1 cut(s)	106
BcgI	CGANNNNNNTGC	4 cut(s)	329, 363, 806, 840
BciT130I	CCWGG	1 cut(s)	88
BcnI	CCSGG	2 cut(s)	139, 999
BcoDI	GTCTC	2 cut(s)	136, 1251
BfaI	CTAG	5 cut(s)	228, 461, 1641, 1725, 1854
BfoI	RGCGCY	1 cut(s)	275
BfuAI	ACCTGC	1 cut(s)	1471
BisI	GCNGC	6 cut(s)	165, 168, 171, 467, 498, 825
BlnI	CCTAGG	1 cut(s)	1640
BlpI	GCTNAGC	2 cut(s)	1764, 1779
BlsI	GCNGC	6 cut(s)	166, 169, 172, 468, 499, 826
Bme1390I	CCNGG	3 cut(s)	88, 139, 999
Bme18I	GGWCC	3 cut(s)	22, 95, 377
BmeT110I	CYCGRG	2 cut(s)	380, 960
BmgT120I	GGNCC	6 cut(s)	22, 95, 377, 479, 480, 1818
BmrFI	CCNGG	3 cut(s)	88, 139, 999
BmsI	GCATC	5 cut(s)	337, 580, 626, 637, 1327
BpiI	GAAGAC	2 cut(s)	140, 901
BplI	GAGNNNNNCTC	2 cut(s)	1231, 1263
BpmI	CTGGAG	2 cut(s)	1635, 1761
Bpu10I	CCTNAGC	1 cut(s)	114
Bpu1102I	GCTNAGC	2 cut(s)	1764, 1779
BpuMI	CCSGG	2 cut(s)	139, 999
BsaHI	GRCGYC	1 cut(s)	272
BsaJI	CCNNGG	6 cut(s)	86, 87, 381, 997, 1640, 1673
BsaWI	WCCGGW	2 cut(s)	64, 257
BsaXI	ACNNNNNCTCC	2 cut(s)	1734, 1764
Bsc4I	CCNNNNNNNGG	2 cut(s)	31, 92
Bse1I	ACTGG	4 cut(s)	972, 1305, 1534, 1628
Bse21I	CCTNAGG	1 cut(s)	1397
BseAI	TCCGGA	1 cut(s)	64
BseBI	CCWGG	1 cut(s)	88
BseDI	CCNNGG	6 cut(s)	86, 87, 381, 997, 1640, 1673
BseGI	GGATG	3 cut(s)	48, 352, 997
BseLI	CCNNNNNNNGG	2 cut(s)	31, 92
BseMII	CTCAG	5 cut(s)	105, 165, 744, 1416, 1793
BseNI	ACTGG	4 cut(s)	972, 1305, 1534, 1628
BseRI	GAGGAG	4 cut(s)	29, 32, 131, 1430
BseSI	GKGCMC	2 cut(s)	483, 931
BseXI	GCAGC	6 cut(s)	151, 154, 157, 478, 509, 811
BseYI	CCCAGC	1 cut(s)	538
BsgI	GTGCAG	2 cut(s)	425, 485
BshFI	GGCC	5 cut(s)	481, 1002, 1222, 1515, 1819
BshNI	GGYRCC	2 cut(s)	271, 1666
BsiHKAI	GWGCWC	2 cut(s)	109, 159
BsiHKCI	CYCGRG	2 cut(s)	380, 960
BsiSI	CCGG	7 cut(s)	65, 102, 110, 139, 258, 999, 1769
BslFI	GGGAC	2 cut(s)	104, 1061
BslI	CCNNNNNNNGG	2 cut(s)	31, 92
BsmAI	GTCTC	2 cut(s)	136, 1251
BsmFI	GGGAC	2 cut(s)	104, 1061
BsmI	GAATGC	2 cut(s)	189, 396
BsnI	GGCC	5 cut(s)	481, 1002, 1222, 1515, 1819
BsoBI	CYCGRG	2 cut(s)	380, 960
Bsp120I	GGGCCC	1 cut(s)	479
Bsp1286I	GDGCHC	6 cut(s)	72, 109, 159, 483, 891, 931
Bsp13I	TCCGGA	1 cut(s)	64
Bsp143I	GATC	4 cut(s)	7, 439, 1345, 1555
Bsp1720I	GCTNAGC	2 cut(s)	1764, 1779
BspACI	CCGC	8 cut(s)	5, 40, 99, 151, 206, 251, 716, 1850
BspANI	GGCC	5 cut(s)	481, 1002, 1222, 1515, 1819
BspCNI	CTCAG	5 cut(s)	106, 166, 745, 1417, 1792
BspEI	TCCGGA	1 cut(s)	64
BspMI	ACCTGC	1 cut(s)	1471
BspPI	GGATC	2 cut(s)	15, 434
BspT107I	GGYRCC	2 cut(s)	271, 1666
BsrI	ACTGG	4 cut(s)	972, 1305, 1534, 1628
BssECI	CCNNGG	6 cut(s)	86, 87, 381, 997, 1640, 1673
BssMI	GATC	4 cut(s)	7, 439, 1345, 1555
BssNI	GRCGYC	1 cut(s)	272
BssSI	CACGAG	1 cut(s)	11
BssT1I	CCWWGG	2 cut(s)	1640, 1673
Bst2BI	CACGAG	1 cut(s)	11
Bst2UI	CCWGG	1 cut(s)	88
Bst4CI	ACNGT	5 cut(s)	597, 1144, 1187, 1258, 1282
Bst6I	CTCTTC	2 cut(s)	173, 336
BstACI	GRCGYC	1 cut(s)	272
BstAPI	GCANNNNNTGC	1 cut(s)	841
BstC8I	GCNNGC	6 cut(s)	83, 1032, 1071, 1776, 1785, 1817
BstDEI	CTNAG	8 cut(s)	114, 174, 753, 845, 1397, 1425, 1764, 1779
BstEII	GGTNACC	1 cut(s)	1844
BstF5I	GGATG	3 cut(s)	48, 352, 997
BstH2I	RGCGCY	1 cut(s)	275
BstHHI	GCGC	1 cut(s)	274
BstKTI	GATC	4 cut(s)	10, 442, 1348, 1558
BstMAI	GTCTC	2 cut(s)	136, 1251
BstMBI	GATC	4 cut(s)	7, 439, 1345, 1555
BstNI	CCWGG	1 cut(s)	88
BstNSI	RCATGY	4 cut(s)	571, 1034, 1120, 1787
BstPI	GGTNACC	1 cut(s)	1844
BstSCI	CCNGG	3 cut(s)	86, 137, 997
BstSLI	GKGCMC	2 cut(s)	483, 931
BstV1I	GCAGC	6 cut(s)	151, 154, 157, 478, 509, 811
BstV2I	GAAGAC	2 cut(s)	140, 901
BstXI	CCANNNNNNTGG	2 cut(s)	1144, 1571
Bsu36I	CCTNAGG	1 cut(s)	1397
BsuRI	GGCC	5 cut(s)	481, 1002, 1222, 1515, 1819
BtsCI	GGATG	3 cut(s)	48, 352, 997
BtsI	GCAGTG	5 cut(s)	35, 413, 903, 929, 1014
BtsIMutI	CAGTG	7 cut(s)	35, 413, 614, 903, 929, 1014, 1278
BveI	ACCTGC	1 cut(s)	1471
Cac8I	GCNNGC	6 cut(s)	83, 1032, 1071, 1776, 1785, 1817
CaiI	CAGNNNCTG	2 cut(s)	827, 1613
CfoI	GCGC	1 cut(s)	274
Cfr13I	GGNCC	6 cut(s)	22, 95, 377, 479, 480, 1818
Csp6I	GTAC	3 cut(s)	662, 977, 1667
CspCI	CAANNNNNGTGG	2 cut(s)	1212, 1247
CviAII	CATG	7 cut(s)	160, 470, 568, 1031, 1117, 1696, 1784
CviQI	GTAC	3 cut(s)	662, 977, 1667
DdeI	CTNAG	8 cut(s)	114, 174, 753, 845, 1397, 1425, 1764, 1779
DinI	GGCGCC	1 cut(s)	273
DpnI	GATC	4 cut(s)	9, 441, 1347, 1557
DpnII	GATC	4 cut(s)	7, 439, 1345, 1555
DraI	TTTAAA	1 cut(s)	1125
EaeI	YGGCCR	1 cut(s)	1220
Eam1104I	CTCTTC	2 cut(s)	173, 336
EarI	CTCTTC	2 cut(s)	173, 336
EciI	GGCGGA	2 cut(s)	20, 195
Ecl136II	GAGCTC	2 cut(s)	107, 157
Eco130I	CCWWGG	2 cut(s)	1640, 1673
Eco147I	AGGCCT	1 cut(s)	1515
Eco24I	GRGCYC	5 cut(s)	72, 109, 159, 483, 891
Eco47I	GGWCC	3 cut(s)	22, 95, 377
Eco53kI	GAGCTC	2 cut(s)	107, 157
Eco57I	CTGAAG	4 cut(s)	131, 663, 1538, 1707
Eco81I	CCTNAGG	1 cut(s)	1397
Eco88I	CYCGRG	2 cut(s)	380, 960
Eco91I	GGTNACC	1 cut(s)	1844
EcoICRI	GAGCTC	2 cut(s)	107, 157
EcoO109I	RGGNCCY	2 cut(s)	22, 480
EcoO65I	GGTNACC	1 cut(s)	1844
EcoRI	GAATTC	1 cut(s)	55
EcoRII	CCWGG	1 cut(s)	86
EcoT14I	CCWWGG	2 cut(s)	1640, 1673
EcoT22I	ATGCAT	1 cut(s)	573
EcoT38I	GRGCYC	5 cut(s)	72, 109, 159, 483, 891
EgeI	GGCGCC	1 cut(s)	273
EheI	GGCGCC	1 cut(s)	273
ErhI	CCWWGG	2 cut(s)	1640, 1673
FaeI	CATG	7 cut(s)	163, 473, 571, 1034, 1120, 1699, 1787
FalI	AAGNNNNNCTT	2 cut(s)	885, 917
FaqI	GGGAC	2 cut(s)	104, 1061
FatI	CATG	7 cut(s)	159, 469, 567, 1030, 1116, 1695, 1783
FauI	CCCGC	4 cut(s)	106, 144, 244, 1857
Fnu4HI	GCNGC	6 cut(s)	165, 168, 171, 467, 498, 825
FokI	GGATG	3 cut(s)	55, 359, 1004
FriOI	GRGCYC	5 cut(s)	72, 109, 159, 483, 891
Fsp4HI	GCNGC	6 cut(s)	165, 168, 171, 467, 498, 825
FspBI	CTAG	5 cut(s)	228, 461, 1641, 1725, 1854
GlaI	GCGC	1 cut(s)	273
GluI	GCNGC	6 cut(s)	165, 168, 171, 467, 498, 825
GsaI	CCCAGC	1 cut(s)	542
GsuI	CTGGAG	2 cut(s)	1635, 1761
HaeII	RGCGCY	1 cut(s)	275
HaeIII	GGCC	5 cut(s)	481, 1002, 1222, 1515, 1819
HapII	CCGG	7 cut(s)	65, 102, 110, 139, 258, 999, 1769
HhaI	GCGC	1 cut(s)	274
Hin1I	GRCGYC	1 cut(s)	272
Hin1II	CATG	7 cut(s)	163, 473, 571, 1034, 1120, 1699, 1787
Hin6I	GCGC	1 cut(s)	272
HinP1I	GCGC	1 cut(s)	272
HincII	GTYRAC	1 cut(s)	1435
HindII	GTYRAC	1 cut(s)	1435
HpaII	CCGG	7 cut(s)	65, 102, 110, 139, 258, 999, 1769
HphI	GGTGA	5 cut(s)	51, 216, 692, 730, 1856
Hpy166II	GTNNAC	5 cut(s)	662, 1051, 1435, 1680, 1798
Hpy188I	TCNGA	8 cut(s)	29, 127, 377, 439, 985, 1326, 1350, 1588
Hpy188III	TCNNGA	9 cut(s)	11, 65, 443, 960, 1271, 1286, 1614, 1685, 1740
Hpy8I	GTNNAC	5 cut(s)	662, 1051, 1435, 1680, 1798
Hpy99I	CGWCG	2 cut(s)	127, 289
HpyAV	CCTTC	9 cut(s)	219, 385, 467, 1298, 1302, 1373, 1423, 1513, 1540
HpyCH4III	ACNGT	5 cut(s)	597, 1144, 1187, 1258, 1282
HpyCH4IV	ACGT	2 cut(s)	279, 1410
HpyF3I	CTNAG	8 cut(s)	114, 174, 753, 845, 1397, 1425, 1764, 1779
HpySE526I	ACGT	2 cut(s)	279, 1410
Hsp92I	GRCGYC	1 cut(s)	272
Hsp92II	CATG	7 cut(s)	163, 473, 571, 1034, 1120, 1699, 1787
HspAI	GCGC	1 cut(s)	272
KasI	GGCGCC	1 cut(s)	271
Kpn2I	TCCGGA	1 cut(s)	64
KpnI	GGTACC	1 cut(s)	1670
Kzo9I	GATC	4 cut(s)	7, 439, 1345, 1555
LmnI	GCTCC	8 cut(s)	67, 104, 112, 118, 154, 894, 1024, 1771
Lsp1109I	GCAGC	6 cut(s)	151, 154, 157, 478, 509, 811
LweI	GCATC	5 cut(s)	337, 580, 626, 637, 1327
MaeI	CTAG	5 cut(s)	228, 461, 1641, 1725, 1854
MaeII	ACGT	2 cut(s)	279, 1410
MaeIII	GTNAC	5 cut(s)	385, 795, 1012, 1750, 1844
MalI	GATC	4 cut(s)	9, 441, 1347, 1557
MboI	GATC	4 cut(s)	7, 439, 1345, 1555
MboII	GAAGA	6 cut(s)	145, 190, 353, 656, 901, 1700
MfeI	CAATTG	1 cut(s)	1834
MhlI	GDGCHC	6 cut(s)	72, 109, 159, 483, 891, 931
MlsI	TGGCCA	1 cut(s)	1222
MluNI	TGGCCA	1 cut(s)	1222
Mly113I	GGCGCC	1 cut(s)	272
MlyI	GAGTC	2 cut(s)	1253, 1675
MmeI	TCCRAC	3 cut(s)	105, 1132, 1445
Mox20I	TGGCCA	1 cut(s)	1222
Mph1103I	ATGCAT	1 cut(s)	573
MroI	TCCGGA	1 cut(s)	64
MroXI	GAANNNNTTC	1 cut(s)	1383
MscI	TGGCCA	1 cut(s)	1222
MseI	TTAA	2 cut(s)	1124, 1569
MslI	CAYNNNNRTG	3 cut(s)	401, 474, 1569
Msp20I	TGGCCA	1 cut(s)	1222
MspA1I	CMGCKG	3 cut(s)	151, 542, 824
MspI	CCGG	7 cut(s)	65, 102, 110, 139, 258, 999, 1769
MspR9I	CCNGG	3 cut(s)	88, 139, 999
MunI	CAATTG	1 cut(s)	1834
Mva1269I	GAATGC	2 cut(s)	189, 396
MvaI	CCWGG	1 cut(s)	88
NarI	GGCGCC	1 cut(s)	272
NciI	CCSGG	2 cut(s)	139, 999
NdeII	GATC	4 cut(s)	7, 439, 1345, 1555
NlaIII	CATG	7 cut(s)	163, 473, 571, 1034, 1120, 1699, 1787
NmuCI	GTSAC	4 cut(s)	795, 1012, 1750, 1844
NsiI	ATGCAT	1 cut(s)	573
NspI	RCATGY	4 cut(s)	571, 1034, 1120, 1787
PaeI	GCATGC	2 cut(s)	1034, 1787
PaeR7I	CTCGAG	1 cut(s)	960
PasI	CCCWGGG	1 cut(s)	87
PceI	AGGCCT	1 cut(s)	1515
PcsI	WCGNNNNNNNCGW	1 cut(s)	1819
PctI	GAATGC	2 cut(s)	189, 396
PdmI	GAANNNNTTC	1 cut(s)	1383
PfeI	GAWTC	8 cut(s)	818, 1155, 1172, 1313, 1321, 1523, 1624, 1736
PflFI	GACNNNGTC	1 cut(s)	595
PkrI	GCNGC	6 cut(s)	166, 169, 172, 468, 499, 826
PleI	GAGTC	2 cut(s)	1252, 1675
PluTI	GGCGCC	1 cut(s)	275
PpsI	GAGTC	2 cut(s)	1252, 1675
PpuMI	RGGWCCY	1 cut(s)	22
Psp124BI	GAGCTC	2 cut(s)	109, 159
Psp5II	RGGWCCY	1 cut(s)	22
Psp6I	CCWGG	1 cut(s)	86
PspEI	GGTNACC	1 cut(s)	1844
PspFI	CCCAGC	1 cut(s)	538
PspGI	CCWGG	1 cut(s)	86
PspOMI	GGGCCC	1 cut(s)	479
PspPI	GGNCC	6 cut(s)	22, 95, 377, 479, 480, 1818
PspPPI	RGGWCCY	1 cut(s)	22
PstNI	CAGNNNCTG	2 cut(s)	827, 1613
PsyI	GACNNNGTC	1 cut(s)	595
PvuII	CAGCTG	2 cut(s)	542, 824
RsaI	GTAC	3 cut(s)	663, 978, 1668
RsaNI	GTAC	3 cut(s)	662, 977, 1667
RseI	CAYNNNNRTG	3 cut(s)	401, 474, 1569
SacI	GAGCTC	2 cut(s)	109, 159
SaqAI	TTAA	2 cut(s)	1124, 1569
SatI	GCNGC	6 cut(s)	165, 168, 171, 467, 498, 825
Sau3AI	GATC	4 cut(s)	7, 439, 1345, 1555
Sau96I	GGNCC	6 cut(s)	22, 95, 377, 479, 480, 1818
SchI	GAGTC	2 cut(s)	1253, 1675
ScrFI	CCNGG	3 cut(s)	88, 139, 999
SduI	GDGCHC	6 cut(s)	72, 109, 159, 483, 891, 931
SfaNI	GCATC	5 cut(s)	337, 580, 626, 637, 1327
SfoI	GGCGCC	1 cut(s)	273
Sfr274I	CTCGAG	1 cut(s)	960
SinI	GGWCC	3 cut(s)	22, 95, 377
SlaI	CTCGAG	1 cut(s)	960
SmiMI	CAYNNNNRTG	3 cut(s)	401, 474, 1569
SmlI	CTYRAG	1 cut(s)	960
SmoI	CTYRAG	1 cut(s)	960
SphI	GCATGC	2 cut(s)	1034, 1787
SseBI	AGGCCT	1 cut(s)	1515
SsiI	CCGC	8 cut(s)	5, 40, 99, 151, 206, 251, 716, 1850
SspDI	GGCGCC	1 cut(s)	271
SspMI	CTAG	5 cut(s)	228, 461, 1641, 1725, 1854
SstI	GAGCTC	2 cut(s)	109, 159
StuI	AGGCCT	1 cut(s)	1515
StyD4I	CCNGG	3 cut(s)	86, 137, 997
StyI	CCWWGG	2 cut(s)	1640, 1673
TaaI	ACNGT	5 cut(s)	597, 1144, 1187, 1258, 1282
TaiI	ACGT	2 cut(s)	282, 1413
TaqI	TCGA	5 cut(s)	365, 620, 816, 961, 1089
TatI	WGTACW	1 cut(s)	661
TfiI	GAWTC	8 cut(s)	818, 1155, 1172, 1313, 1321, 1523, 1624, 1736
Tru1I	TTAA	2 cut(s)	1124, 1569
Tru9I	TTAA	2 cut(s)	1124, 1569
TscAI	CASTG	7 cut(s)	42, 413, 614, 903, 936, 1021, 1285
TseFI	GTSAC	4 cut(s)	795, 1012, 1750, 1844
TseI	GCWGC	6 cut(s)	164, 167, 170, 466, 497, 824
Tsp45I	GTSAC	4 cut(s)	795, 1012, 1750, 1844
TspDTI	ATGAA	6 cut(s)	486, 762, 795, 1311, 1376, 1749
TspGWI	ACGGA	1 cut(s)	108
TspRI	CASTG	7 cut(s)	42, 413, 614, 903, 936, 1021, 1285
Tth111I	GACNNNGTC	1 cut(s)	595
VpaK11BI	GGWCC	3 cut(s)	22, 95, 377
XapI	RAATTY	2 cut(s)	55, 319
XceI	RCATGY	4 cut(s)	571, 1034, 1120, 1787
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1610
XhoI	CTCGAG	1 cut(s)	960
XmaJI	CCTAGG	1 cut(s)	1640
XmnI	GAANNNNTTC	1 cut(s)	1383
XspI	CTAG	5 cut(s)	228, 461, 1641, 1725, 1854
Zsp2I	ATGCAT	1 cut(s)	573

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.