RLG00000013449

DUF4205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012146
Physical Location & Seq
Reverse (-)
31624725 .. 31632812
8088 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013449

Sequence Viewer

Length: 1833 bp
ATGGCGGATCAGGAGGAGGACCTGCAAATGGCACTGAGGATGAGTATGCAGAATTCGCCGCCGGAGCCCAAGCGGAGCAAGCCCAGGGACGCCGATTCGCCGGCGGAGGAGTCGTCGGAAGTCAAGACCCGGCGACTTCAGCGAGAGCTCATGGCTACTGCAGCCGAGAGGAGAATGCTCGACGCCAGGGCCACCGCTCCTCCTACTCCTCCTCCGGCTGCCTCGCCGGTCTCCGTTTCCAGGTCTGCGGCGGGCTCGGGTTCGGGTTTGGCGCCCAATGTGGTTAGGAAAGAGAGGCAATTGGGCGCGGAGTTGAGCGTGGGGGATGCCAAGCAGTTGTTTTCGATGGTGTTTGGGGCTGAGGTTTCCAAGGGCATTCTTGCGCAGTGGAGTAATCAGGGTATAAGGTTTAGCCCTGATCCAGAAACATCGATGGGTCTAGTGCAGCATGAAGGTGGGCCCTGTGGCGTTTTAGCAGCCATTCAAGCACTTGTTCTCAAATACCTCCTTTTCTACCCAGACGGACTAGGTAAAGGTGCACCAAACATGCATCACAATTTGGGTTCAAGGACACTTTCTAGTCATTATGTTGCATCGACTAACTTTTCCTCACTTTCTGAAGATGCAAAAGCAAGAGCATTGCTTAGAAGTATGGGTGAGATATTGTTCTCTTGTGGAAGTAATAAAAGGGCAGTGATTGCAACTTTGAGTGTTATTGGAGATGATGTTGAGAGGTTTGAAGGCAGCAAGGATGAAGTAATATCAAAATCACTTGAGGGCCTTTCTATTGAATCAGCTGCTGACTTGCATAAAGTGCTAAGAGTTAACACATACACAACGCAAGAAAGTGCATTACGGGGGCTAGAAGCAGTGATTCCTGTTTTCCGAAGTCGTATGGGGGCATTGCTGTTCCTTATTTCTGCTTTGCTTTCACGAGGACTGGACTTAATCCAGGCTGATAGGGATGATCCTAGCCTACCTCTTGTCACTGCTCCATTTGGGCATGCCTCACAGGAAATTGTGAACTTACTGCTCTGCGGGCAGGCCGTCCCAAATGTTTTCGACGGAAAGATGGATTTAGGTGGTGGTATGTCTTTAAAGGGCATATCCAGAACCGTGGAAGTTGGATTCCTCACACTGTTGGAGTCCCTTAATTTCTGCAAAGTTGGCCAGTATTTGAAATGCCCCAAATGGCCAATTTGGGTAGTTGGAAGTGAGTCTCATTATACAGTTCTCTTTGCTCCTGATACCAGTGTCCAGGATGAGAATGAACTGGAAGGAAGGGAATCACAGATTCGGAAAGCTTTTGATGCACAAGATCAGAGTGGGGGTGGTGGTTTCATAAGTGTTGAAGGCTTTCATCAAGTCCTTAGGGAAACTAATGTAAAAGTTCCAAGTGAGAAGGTTGATCTTCTCTGCAGCACAGGATTTATTGTATGGAGTGAGTTCTGGCAGGTCATTTTGGATCTGGACAAGAGCATGGGAGGCCTCAAGGATTCAACTGGATTAATGGGCAAGAAGGTCTTTGATCTTTACCATTTTAATGGAATTGCAAAATCAGATCTGAATGGAAGCCAGGTAAGCTCTGGAGGCGAGACTCCAGTGCAAAGACCTAGGCTCACAAAATTGAGGGTGTCAGTTCCTCCAAGATGGACTCCTGAAGAATATATGACAGATGTAGCAGTGTCCTCTAGTGGGAATGAATCCAAAGGTGAAGTCACAGAAATTTCTAAGCCAGAGCCTCCTCAGCATGCACCTTTGGTGGACTGCATAAGAACGCGCTGGCCCCGCGCTGTTTGCAATTGGGTAGGGGATGCGCCTAGTATTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4205

Protein Analysis

611

Amino Acids

66.04

Weight (kDa)

5.73

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MINDY-3_4_CD PF13898 116 - 422 1.6e-57 Deubiquitinating enzyme MINDY-3/4, conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012146)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43690
fragaria_vesca FvH4_2g13910 FvH4_2g13910
malus_domestica MD05G1023700.v1.1 MD10G1024300.v1.1
prunus_persica Prupe.8G028200_v2.0.a1 Prupe.8G028200_v2.0.a1
pyrus_communis pycom05g01520 pycom10g01610
rosa_chinensis RchiOBHm_Chr6g0275661
rosa_laevigata RLG00000013449
rosa_multiflora Rmu_sc0011044.1_g000001
rosa_roxburghii Rroxscaffold_7G00193050
rosa_rugosa Rorug06G0095400
rosa_samantha Rh6AG207300 Rh6BG209900 Rh6CG213300 Rh6DG203000
rosa_wichuraiana Rw6G018070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 384
Acc36I ACCTGC 2 cut(s) 30, 1444
AccB1I GGYRCC 1 cut(s) 271
AccBSI CCGCTC 1 cut(s) 197
AccII CGCG 3 cut(s) 308, 1780, 1791
AclWI GGATC 4 cut(s) 15, 413, 962, 1473
AcoI YGGCCR 2 cut(s) 1168, 1193
AcsI RAATTY 2 cut(s) 52, 1725
AcuI CTGAAG 3 cut(s) 122, 639, 1680
AcyI GRCGYC 3 cut(s) 90, 183, 272
AfiI CCNNNNNNNGG 3 cut(s) 28, 240, 1695
AgsI TTSAA 7 cut(s) 485, 567, 740, 791, 1180, 1352, 1500
AjnI CCWGG 6 cut(s) 83, 185, 239, 951, 1257, 1575
AluBI AGCT 4 cut(s) 148, 797, 1304, 1584
AluI AGCT 4 cut(s) 148, 797, 1304, 1584
Alw21I GWGCWC 2 cut(s) 150, 541
Alw26I GTCTC 3 cut(s) 235, 1224, 1589
Alw44I GTGCAC 1 cut(s) 537
AlwI GGATC 4 cut(s) 15, 413, 962, 1473
AlwNI CAGNNNCTG 1 cut(s) 800
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 8 cut(s) 189, 458, 778, 1044, 1168, 1193, 1486, 1784
ApaI GGGCCC 1 cut(s) 462
ApaLI GTGCAC 1 cut(s) 537
ApeKI GCWGC 7 cut(s) 161, 218, 445, 476, 744, 797, 1419
ApoI RAATTY 2 cut(s) 52, 1725
ArsI GACNNNNNNTTYG 2 cut(s) 1701, 1733
AseI ATTAAT 1 cut(s) 1508
Asp700I GAANNNNTTC 1 cut(s) 1356
AspA2I CCTAGG 1 cut(s) 1613
AspLEI GCGC 6 cut(s) 274, 308, 385, 1782, 1793, 1819
AspS9I GGNCC 6 cut(s) 19, 189, 458, 459, 778, 1785
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 2 cut(s) 668, 1724
AvaI CYCGRG 1 cut(s) 256
AvaII GGWCC 1 cut(s) 19
AvrII CCTAGG 1 cut(s) 1613
AxyI CCTNAGG 1 cut(s) 1370
BaeGI GKGCMC 2 cut(s) 462, 541
BaeI ACNNNNGTAYC 2 cut(s) 1239, 1272
BalI TGGCCA 2 cut(s) 1170, 1195
BanI GGYRCC 1 cut(s) 271
BanII GRGCYC 4 cut(s) 69, 150, 257, 462
BauI CACGAG 1 cut(s) 933
Bbv12I GWGCWC 2 cut(s) 150, 541
BbvCI CCTCAGC 2 cut(s) 360, 1746
BbvI GCAGC 7 cut(s) 173, 205, 457, 488, 756, 784, 1431
BccI CCATC 4 cut(s) 340, 427, 1066, 1644
BceAI ACGGC 1 cut(s) 1031
BciT130I CCWGG 6 cut(s) 85, 187, 241, 953, 1259, 1577
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 3 cut(s) 235, 1224, 1589
BfaI CTAG 8 cut(s) 440, 527, 579, 863, 972, 1614, 1692, 1821
BfmI CTRYAG 2 cut(s) 159, 1417
BfoI RGCGCY 1 cut(s) 275
BfuAI ACCTGC 2 cut(s) 30, 1444
BglII AGATCT 1 cut(s) 1561
BisI GCNGC 9 cut(s) 59, 162, 219, 249, 446, 477, 745, 798, 1420
BlnI CCTAGG 1 cut(s) 1613
BlsI GCNGC 9 cut(s) 60, 163, 220, 250, 447, 478, 746, 799, 1421
Bme1390I CCNGG 7 cut(s) 85, 130, 187, 241, 953, 1259, 1577
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 6 cut(s) 19, 189, 458, 459, 778, 1785
BmiI GGNNCC 4 cut(s) 66, 273, 460, 1787
BmrFI CCNGG 7 cut(s) 85, 130, 187, 241, 953, 1259, 1577
BmsI GCATC 6 cut(s) 316, 559, 602, 613, 1300, 1804
BpmI CTGGAG 2 cut(s) 1584, 1608
Bpu10I CCTNAGC 2 cut(s) 360, 1746
BpuEI CTTGAG 2 cut(s) 794, 1475
BpuMI CCSGG 1 cut(s) 130
Bsa29I ATCGAT 1 cut(s) 431
BsaHI GRCGYC 3 cut(s) 90, 183, 272
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 6 cut(s) 83, 84, 186, 369, 1116, 1613
BsaXI ACNNNNNCTCC 8 cut(s) 98, 128, 184, 196, 214, 226, 711, 741
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 240, 1695
Bse118I RCCGGY 2 cut(s) 100, 226
Bse1I ACTGG 6 cut(s) 945, 1171, 1251, 1278, 1507, 1601
Bse21I CCTNAGG 1 cut(s) 1370
Bse3DI GCAATG 2 cut(s) 638, 902
BseBI CCWGG 6 cut(s) 85, 187, 241, 953, 1259, 1577
BseCI ATCGAT 1 cut(s) 431
BseDI CCNNGG 6 cut(s) 83, 84, 186, 369, 1116, 1613
BseGI GGATG 6 cut(s) 45, 331, 757, 970, 1267, 1819
BseLI CCNNNNNNNGG 3 cut(s) 28, 240, 1695
BseMI GCAATG 2 cut(s) 638, 902
BseMII CTCAG 3 cut(s) 26, 351, 1760
BseNI ACTGG 6 cut(s) 945, 1171, 1251, 1278, 1507, 1601
BseRI GAGGAG 7 cut(s) 29, 122, 184, 189, 198, 201, 1734
BseSI GKGCMC 2 cut(s) 462, 541
BseXI GCAGC 7 cut(s) 173, 205, 457, 488, 756, 784, 1431
BsgI GTGCAG 1 cut(s) 464
Bsh1236I CGCG 3 cut(s) 308, 1780, 1791
BshFI GGCC 8 cut(s) 191, 460, 780, 1046, 1170, 1195, 1488, 1786
BshNI GGYRCC 1 cut(s) 271
BshVI ATCGAT 1 cut(s) 431
BsiHKAI GWGCWC 2 cut(s) 150, 541
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 5 cut(s) 62, 101, 130, 215, 227
BslFI GGGAC 3 cut(s) 101, 1034, 1132
BslI CCNNNNNNNGG 3 cut(s) 28, 240, 1695
BsmAI GTCTC 3 cut(s) 235, 1224, 1589
BsmFI GGGAC 3 cut(s) 101, 1034, 1132
BsmI GAATGC 2 cut(s) 180, 375
BsnI GGCC 8 cut(s) 191, 460, 780, 1046, 1170, 1195, 1488, 1786
Bso31I GGTCTC 1 cut(s) 235
BsoBI CYCGRG 1 cut(s) 256
Bsp120I GGGCCC 1 cut(s) 458
Bsp1286I GDGCHC 5 cut(s) 69, 150, 257, 462, 541
Bsp143I GATC 8 cut(s) 7, 418, 967, 1318, 1408, 1465, 1528, 1561
BspANI GGCC 8 cut(s) 191, 460, 780, 1046, 1170, 1195, 1488, 1786
BspCNI CTCAG 3 cut(s) 27, 352, 1759
BspDI ATCGAT 1 cut(s) 431
BspFNI CGCG 3 cut(s) 308, 1780, 1791
BspLI GGNNCC 4 cut(s) 66, 273, 460, 1787
BspMAI CTGCAG 2 cut(s) 163, 1421
BspMI ACCTGC 2 cut(s) 30, 1444
BspPI GGATC 4 cut(s) 15, 413, 962, 1473
BspT107I GGYRCC 1 cut(s) 271
BspTNI GGTCTC 1 cut(s) 235
BsrBI CCGCTC 1 cut(s) 197
BsrDI GCAATG 2 cut(s) 638, 902
BsrFI RCCGGY 2 cut(s) 100, 226
BsrI ACTGG 6 cut(s) 945, 1171, 1251, 1278, 1507, 1601
BssAI RCCGGY 2 cut(s) 100, 226
BssECI CCNNGG 6 cut(s) 83, 84, 186, 369, 1116, 1613
BssMI GATC 8 cut(s) 7, 418, 967, 1318, 1408, 1465, 1528, 1561
BssNI GRCGYC 3 cut(s) 90, 183, 272
BssSI CACGAG 1 cut(s) 933
BssT1I CCWWGG 2 cut(s) 369, 1613
Bst2BI CACGAG 1 cut(s) 933
Bst2UI CCWGG 6 cut(s) 85, 187, 241, 953, 1259, 1577
Bst4CI ACNGT 3 cut(s) 1117, 1140, 1231
BstACI GRCGYC 3 cut(s) 90, 183, 272
BstAPI GCANNNNNTGC 2 cut(s) 698, 814
BstC8I GCNNGC 8 cut(s) 80, 102, 253, 1005, 1040, 1044, 1752, 1784
BstDEI CTNAG 7 cut(s) 35, 360, 644, 818, 1370, 1731, 1746
BstDSI CCRYGG 1 cut(s) 1116
BstF5I GGATG 6 cut(s) 45, 331, 757, 970, 1267, 1819
BstFNI CGCG 3 cut(s) 308, 1780, 1791
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 6 cut(s) 274, 308, 385, 1782, 1793, 1819
BstKTI GATC 8 cut(s) 10, 421, 970, 1321, 1411, 1468, 1531, 1564
BstMAI GTCTC 3 cut(s) 235, 1224, 1589
BstMBI GATC 8 cut(s) 7, 418, 967, 1318, 1408, 1465, 1528, 1561
BstNI CCWGG 6 cut(s) 85, 187, 241, 953, 1259, 1577
BstNSI RCATGY 3 cut(s) 550, 1007, 1754
BstSCI CCNGG 7 cut(s) 83, 128, 185, 239, 951, 1257, 1575
BstSFI CTRYAG 2 cut(s) 159, 1417
BstSLI GKGCMC 2 cut(s) 462, 541
BstUI CGCG 3 cut(s) 308, 1780, 1791
BstV1I GCAGC 7 cut(s) 173, 205, 457, 488, 756, 784, 1431
BstX2I RGATCY 2 cut(s) 1465, 1561
BstXI CCANNNNNNTGG 2 cut(s) 1117, 1544
BstYI RGATCY 2 cut(s) 1465, 1561
Bsu15I ATCGAT 1 cut(s) 431
Bsu36I CCTNAGG 1 cut(s) 1370
BsuRI GGCC 8 cut(s) 191, 460, 780, 1046, 1170, 1195, 1488, 1786
BsuTUI ATCGAT 1 cut(s) 431
BtgI CCRYGG 1 cut(s) 1116
BtsCI GGATG 6 cut(s) 45, 331, 757, 970, 1267, 1819
BtsI GCAGTG 5 cut(s) 392, 699, 876, 987, 1689
BtsIMutI CAGTG 9 cut(s) 32, 392, 699, 876, 987, 1136, 1258, 1608, 1689
BveI ACCTGC 2 cut(s) 30, 1444
Cac8I GCNNGC 8 cut(s) 80, 102, 253, 1005, 1040, 1044, 1752, 1784
CaiI CAGNNNCTG 1 cut(s) 800
CfoI GCGC 6 cut(s) 274, 308, 385, 1782, 1793, 1819
Cfr10I RCCGGY 2 cut(s) 100, 226
Cfr13I GGNCC 6 cut(s) 19, 189, 458, 459, 778, 1785
ClaI ATCGAT 1 cut(s) 431
CseI GACGC 2 cut(s) 98, 191
CviAII CATG 6 cut(s) 151, 449, 547, 1004, 1480, 1751
DdeI CTNAG 7 cut(s) 35, 360, 644, 818, 1370, 1731, 1746
DinI GGCGCC 1 cut(s) 273
DpnI GATC 8 cut(s) 9, 420, 969, 1320, 1410, 1467, 1530, 1563
DpnII GATC 8 cut(s) 7, 418, 967, 1318, 1408, 1465, 1528, 1561
DraI TTTAAA 1 cut(s) 1098
EaeI YGGCCR 2 cut(s) 1168, 1193
EciI GGCGGA 2 cut(s) 20, 119
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 369, 1613
Eco147I AGGCCT 1 cut(s) 1488
Eco24I GRGCYC 4 cut(s) 69, 150, 257, 462
Eco31I GGTCTC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 3 cut(s) 122, 639, 1680
Eco81I CCTNAGG 1 cut(s) 1370
Eco88I CYCGRG 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 3 cut(s) 19, 459, 778
EcoRI GAATTC 1 cut(s) 52
EcoRII CCWGG 6 cut(s) 83, 185, 239, 951, 1257, 1575
EcoT14I CCWWGG 2 cut(s) 369, 1613
EcoT22I ATGCAT 1 cut(s) 552
EcoT38I GRGCYC 4 cut(s) 69, 150, 257, 462
EgeI GGCGCC 1 cut(s) 273
EheI GGCGCC 1 cut(s) 273
ErhI CCWWGG 2 cut(s) 369, 1613
FaeI CATG 6 cut(s) 154, 452, 550, 1007, 1483, 1754
FalI AAGNNNNNCTT 2 cut(s) 1508, 1540
FaqI GGGAC 3 cut(s) 101, 1034, 1132
FatI CATG 6 cut(s) 150, 448, 546, 1003, 1479, 1750
FauI CCCGC 3 cut(s) 244, 1031, 1796
Fnu4HI GCNGC 9 cut(s) 59, 162, 219, 249, 446, 477, 745, 798, 1420
FokI GGATG 6 cut(s) 52, 338, 764, 977, 1274, 1826
FriOI GRGCYC 4 cut(s) 69, 150, 257, 462
Fsp4HI GCNGC 9 cut(s) 59, 162, 219, 249, 446, 477, 745, 798, 1420
FspBI CTAG 8 cut(s) 440, 527, 579, 863, 972, 1614, 1692, 1821
FspI TGCGCA 1 cut(s) 384
GlaI GCGC 6 cut(s) 273, 307, 384, 1781, 1792, 1818
GluI GCNGC 9 cut(s) 59, 162, 219, 249, 446, 477, 745, 798, 1420
GsuI CTGGAG 2 cut(s) 1584, 1608
HaeII RGCGCY 1 cut(s) 275
HaeIII GGCC 8 cut(s) 191, 460, 780, 1046, 1170, 1195, 1488, 1786
HapII CCGG 5 cut(s) 62, 101, 130, 215, 227
HgaI GACGC 2 cut(s) 98, 191
HhaI GCGC 6 cut(s) 274, 308, 385, 1782, 1793, 1819
Hin1I GRCGYC 3 cut(s) 90, 183, 272
Hin1II CATG 6 cut(s) 154, 452, 550, 1007, 1483, 1754
Hin6I GCGC 6 cut(s) 272, 306, 383, 1780, 1791, 1817
HinP1I GCGC 6 cut(s) 272, 306, 383, 1780, 1791, 1817
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HindIII AAGCTT 1 cut(s) 1302
HpaI GTTAAC 1 cut(s) 826
HpaII CCGG 5 cut(s) 62, 101, 130, 215, 227
HphI GGTGA 2 cut(s) 668, 1724
Hpy166II GTNNAC 4 cut(s) 539, 826, 1024, 1765
Hpy188I TCNGA 8 cut(s) 118, 619, 887, 1299, 1323, 1561, 1566, 1832
Hpy188III TCNNGA 9 cut(s) 11, 124, 422, 933, 1110, 1244, 1469, 1587, 1658
Hpy8I GTNNAC 4 cut(s) 539, 826, 1024, 1765
Hpy99I CGWCG 3 cut(s) 118, 185, 1067
HpyAV CCTTC 7 cut(s) 446, 734, 1271, 1275, 1346, 1396, 1513
HpyCH4III ACNGT 3 cut(s) 1117, 1140, 1231
HpyF3I CTNAG 7 cut(s) 35, 360, 644, 818, 1370, 1731, 1746
Hsp92I GRCGYC 3 cut(s) 90, 183, 272
Hsp92II CATG 6 cut(s) 154, 452, 550, 1007, 1483, 1754
HspAI GCGC 6 cut(s) 272, 306, 383, 1780, 1791, 1817
KasI GGCGCC 1 cut(s) 271
KroI GCCGGC 1 cut(s) 100
KroNI GCCGGC 1 cut(s) 102
KspAI GTTAAC 1 cut(s) 826
Kzo9I GATC 8 cut(s) 7, 418, 967, 1318, 1408, 1465, 1528, 1561
LmnI GCTCC 5 cut(s) 64, 75, 202, 997, 1246
Lsp1109I GCAGC 7 cut(s) 173, 205, 457, 488, 756, 784, 1431
LweI GCATC 6 cut(s) 316, 559, 602, 613, 1300, 1804
MaeI CTAG 8 cut(s) 440, 527, 579, 863, 972, 1614, 1692, 1821
MaeIII GTNAC 2 cut(s) 985, 1717
MalI GATC 8 cut(s) 9, 420, 969, 1320, 1410, 1467, 1530, 1563
MbiI CCGCTC 1 cut(s) 197
MboI GATC 8 cut(s) 7, 418, 967, 1318, 1408, 1465, 1528, 1561
MboII GAAGA 3 cut(s) 632, 1403, 1673
MfeI CAATTG 2 cut(s) 299, 1801
MflI RGATCY 2 cut(s) 1465, 1561
MhlI GDGCHC 5 cut(s) 69, 150, 257, 462, 541
MlsI TGGCCA 2 cut(s) 1170, 1195
MluNI TGGCCA 2 cut(s) 1170, 1195
Mly113I GGCGCC 1 cut(s) 272
MlyI GAGTC 5 cut(s) 119, 1154, 1226, 1591, 1648
MmeI TCCRAC 4 cut(s) 96, 1105, 1122, 1189
Mox20I TGGCCA 2 cut(s) 1170, 1195
Mph1103I ATGCAT 1 cut(s) 552
MreI CGCCGGCG 1 cut(s) 100
MroNI GCCGGC 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 1356
MscI TGGCCA 2 cut(s) 1170, 1195
MseI TTAA 6 cut(s) 825, 947, 1097, 1152, 1508, 1542
MslI CAYNNNNRTG 2 cut(s) 453, 1542
Msp20I TGGCCA 2 cut(s) 1170, 1195
MspA1I CMGCKG 1 cut(s) 797
MspI CCGG 5 cut(s) 62, 101, 130, 215, 227
MspR9I CCNGG 7 cut(s) 85, 130, 187, 241, 953, 1259, 1577
MunI CAATTG 2 cut(s) 299, 1801
Mva1269I GAATGC 2 cut(s) 180, 375
MvaI CCWGG 6 cut(s) 85, 187, 241, 953, 1259, 1577
MvnI CGCG 3 cut(s) 308, 1780, 1791
NaeI GCCGGC 1 cut(s) 102
NarI GGCGCC 1 cut(s) 272
NciI CCSGG 1 cut(s) 130
NdeII GATC 8 cut(s) 7, 418, 967, 1318, 1408, 1465, 1528, 1561
NgoMIV GCCGGC 1 cut(s) 100
NlaIII CATG 6 cut(s) 154, 452, 550, 1007, 1483, 1754
NlaIV GGNNCC 4 cut(s) 66, 273, 460, 1787
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 2 cut(s) 985, 1717
NsbI TGCGCA 1 cut(s) 384
NsiI ATGCAT 1 cut(s) 552
NspI RCATGY 3 cut(s) 550, 1007, 1754
PaeI GCATGC 2 cut(s) 1007, 1754
PasI CCCWGGG 1 cut(s) 84
PceI AGGCCT 1 cut(s) 1488
PctI GAATGC 2 cut(s) 180, 375
PdiI GCCGGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 1356
PfeI GAWTC 8 cut(s) 95, 791, 874, 1128, 1286, 1294, 1496, 1703
PfoI TCCNGGA 1 cut(s) 1257
PkrI GCNGC 9 cut(s) 60, 163, 220, 250, 447, 478, 746, 799, 1421
PleI GAGTC 5 cut(s) 118, 1153, 1225, 1591, 1648
PluTI GGCGCC 1 cut(s) 275
PpsI GAGTC 5 cut(s) 118, 1153, 1225, 1591, 1648
PpuMI RGGWCCY 1 cut(s) 19
PshBI ATTAAT 1 cut(s) 1508
Psp124BI GAGCTC 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 6 cut(s) 83, 185, 239, 951, 1257, 1575
PspGI CCWGG 6 cut(s) 83, 185, 239, 951, 1257, 1575
PspN4I GGNNCC 4 cut(s) 66, 273, 460, 1787
PspOMI GGGCCC 1 cut(s) 458
PspPI GGNCC 6 cut(s) 19, 189, 458, 459, 778, 1785
PspPPI RGGWCCY 1 cut(s) 19
PstI CTGCAG 2 cut(s) 163, 1421
PstNI CAGNNNCTG 1 cut(s) 800
PsuI RGATCY 2 cut(s) 1465, 1561
PvuII CAGCTG 1 cut(s) 797
RseI CAYNNNNRTG 2 cut(s) 453, 1542
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 6 cut(s) 825, 947, 1097, 1152, 1508, 1542
SatI GCNGC 9 cut(s) 59, 162, 219, 249, 446, 477, 745, 798, 1420
Sau3AI GATC 8 cut(s) 7, 418, 967, 1318, 1408, 1465, 1528, 1561
Sau96I GGNCC 6 cut(s) 19, 189, 458, 459, 778, 1785
SchI GAGTC 5 cut(s) 119, 1154, 1226, 1591, 1648
ScrFI CCNGG 7 cut(s) 85, 130, 187, 241, 953, 1259, 1577
SduI GDGCHC 5 cut(s) 69, 150, 257, 462, 541
SfaNI GCATC 6 cut(s) 316, 559, 602, 613, 1300, 1804
SfcI CTRYAG 2 cut(s) 159, 1417
SfoI GGCGCC 1 cut(s) 273
SgrAI CRCCGGYG 1 cut(s) 100
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 2 cut(s) 453, 1542
SmlI CTYRAG 2 cut(s) 773, 1490
SmoI CTYRAG 2 cut(s) 773, 1490
SphI GCATGC 2 cut(s) 1007, 1754
SseBI AGGCCT 1 cut(s) 1488
SspDI GGCGCC 1 cut(s) 271
SspMI CTAG 8 cut(s) 440, 527, 579, 863, 972, 1614, 1692, 1821
SstI GAGCTC 1 cut(s) 150
StuI AGGCCT 1 cut(s) 1488
StyD4I CCNGG 7 cut(s) 83, 128, 185, 239, 951, 1257, 1575
StyI CCWWGG 2 cut(s) 369, 1613
TaaI ACNGT 3 cut(s) 1117, 1140, 1231
TaqI TCGA 5 cut(s) 180, 344, 431, 596, 1062
TauI GCSGC 2 cut(s) 61, 251
TfiI GAWTC 8 cut(s) 95, 791, 874, 1128, 1286, 1294, 1496, 1703
Tru1I TTAA 6 cut(s) 825, 947, 1097, 1152, 1508, 1542
Tru9I TTAA 6 cut(s) 825, 947, 1097, 1152, 1508, 1542
TscAI CASTG 9 cut(s) 39, 392, 699, 876, 994, 1143, 1258, 1608, 1689
TseFI GTSAC 2 cut(s) 985, 1717
TseI GCWGC 7 cut(s) 161, 218, 445, 476, 744, 797, 1419
Tsp45I GTSAC 2 cut(s) 985, 1717
TspDTI ATGAA 6 cut(s) 465, 768, 1284, 1330, 1349, 1716
TspGWI ACGGA 3 cut(s) 223, 537, 1080
TspRI CASTG 9 cut(s) 39, 392, 699, 876, 994, 1143, 1258, 1608, 1689
VneI GTGCAC 1 cut(s) 537
VpaK11BI GGWCC 1 cut(s) 19
VspI ATTAAT 1 cut(s) 1508
XapI RAATTY 2 cut(s) 52, 1725
XceI RCATGY 3 cut(s) 550, 1007, 1754
XcmI CCANNNNNNNNNTGG 1 cut(s) 1583
XmaJI CCTAGG 1 cut(s) 1613
XmnI GAANNNNTTC 1 cut(s) 1356
XspI CTAG 8 cut(s) 440, 527, 579, 863, 972, 1614, 1692, 1821
Zsp2I ATGCAT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.