Rw6G018070

DUF4205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012146
Physical Location & Seq
Forward (+)
35818976 .. 35828373
9398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G018070.1

Sequence Viewer

Length: 1830 bp
ATGGCGGATCAGGAGGAGGACCTGCAAATGGCACTGAGGATGAGTATGCAGAATTCGCCGCCGGAGCCCAAGCGGAGCAAGCCCAGGGACGCCGATTCGCCGGCGGAGGAGTCGTCGGAAGTCAAGACCCGGCGACTTCAGCGAGAGCTCATGGCTACTGCAGCCGAGAGGAGAATGCTCGATGCCAGGGCCAACGCTCCTCCTACTCCTCCGGCTGCCTCACCGGTCTCCGTTTCCAGGTCTGCGGCGGGCTCGGGTTCGGGTTTGGTGCCCAATGTGGTTAGGAAAGAGAGGCAATTGGGAGCCGAGTTGAGCGTGGGGGATGCCAAGCAGTTGTTTTCGATGGTGTTTGGGGCTGAGGTTTCCAAGGGCATTCTTGCGCAGTGGAGTAATCAGGGTATAAGGTTTAGCCCTGATCCAGAAACATCGATGGGTCTAGTGCAGCATGAAGGTGGGCCCTGTGGCGTTTTAGCAGCCATTCAAGCACTTGTTCTCAAATACCTCCTTTTCTACCCAGACGGACTAGGTAAAGGTGCACCAAACATGCATCACAATTTGGGTTCAAGGACACTTTCTAGTCATTGTGTTGCATCGACTAACTTTTCCTCACTTTCTGAAGATGCAAAAGCAAGAGCATTGCTTAGAAGTATGAGTGAGATATTGTTCTCATGTGGAAGTAATGAAAGGGCGGTGGTTGCAACTTTGAGTGTTATTGGAGATGACGTTGAGAGGTTTGAAGGCAGCAAGGATGAAGTAATAGCAAAATCACTTGAGGGCCTTTCTATTGAATCAGCTGCTGACTTGCATAAAGTGCTAAGAGTTAACACATACACGACGCAAGAAAGTGCATTACGGGGGCTAGAAGCAGTGATTCCTGTTTTCCGAAGTCGTATGGGGGCATTGCTGTTCCTTATTTCTGCTTTGCTTTCACGAGGACTGGACTTAATCCAGGCTGATAGGGATGATCCCAGCCTACCTCTTGTCACTGCTCCATTTGGGCATGCCTCACAGGAAATTGTGAACTTACTGCTCTGCGGGCAGGCCGTCCCAAATGTTTTCGACGGAAAGATGGATTTAGGTGGTGGTATGTCTTTAAAGGGCATATCCAGAACCGTGGAAGTTGGATTCCTCACACTGTTGGAGTCCCTTAATTTCTGCAAAGTTGGCCAGTATTTGAAATGCCCCAAATGGCCAATTTGGGTAGTTGGAAGTGAGTCTCATTATACAGTTCTCTTTGCTCCTGATACCAGTGTCCAGGATGAGAATGAACTGGAAGGAAGGGAATCACAGATTCGGAAAGCTTTTGATGCACAAGATCAGAGTGGGGGTGGTGGTTTCATTAGTGTTGAAGGCTTTCATCAAGTCCTTAGGGAAACTAATGTAAAAGTTCCAAGTGAGAAGGTTGATCTTCTTTGCAGCACAGGATTTATTGTATGGAGTGAGTTCTGGCAGGTCATTTTGGATCTGGACAAGAGCATGGGAGGCCTCAAGGATTCAACTGGATTAATGGGCAAGAAGGTCTTTGATCTTTACCATTTTAATGGAATTGCAAAATCAGATCTGAATGGAAGCCAGGTAAGCTCTGGAGGCGAGACTCCAGTGCAAAGACCTAGGCTCACAAAATTGAGGGTGTCAGTTCCTCCAAGATGGACTCCTGAAGAATATATGACAGATGTAGCAGTGTCCTCTAGTGGGAATGAATCCAAAAGTCAAGTCACAGAAATTTCTAAGCCAGAGCCTCCTCAGCATGCACCTTTGGTGGACTGCATAAGAACGCGCTGGCCCCGTGCTGTTTGCAATTGGGTAGGGGATGCCCCTAGTATTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4205

Protein Analysis

609

Amino Acids

65.95

Weight (kDa)

5.65

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MINDY-3_4_CD PF13898 115 - 421 2.6e-58 Deubiquitinating enzyme MINDY-3/4, conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012146)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43690
fragaria_vesca FvH4_2g13910 FvH4_2g13910
malus_domestica MD05G1023700.v1.1 MD10G1024300.v1.1
prunus_persica Prupe.8G028200_v2.0.a1 Prupe.8G028200_v2.0.a1
pyrus_communis pycom05g01520 pycom10g01610
rosa_chinensis RchiOBHm_Chr6g0275661
rosa_laevigata RLG00000013449
rosa_multiflora Rmu_sc0011044.1_g000001
rosa_roxburghii Rroxscaffold_7G00193050
rosa_rugosa Rorug06G0095400
rosa_samantha Rh6AG207300 Rh6BG209900 Rh6CG213300 Rh6DG203000
rosa_wichuraiana Rw6G018070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 381
Acc36I ACCTGC 2 cut(s) 30, 1441
AccB1I GGYRCC 1 cut(s) 268
AccII CGCG 1 cut(s) 1777
AciI CCGC 8 cut(s) 5, 59, 73, 104, 245, 248, 689, 1035
AclWI GGATC 4 cut(s) 15, 410, 959, 1470
AcoI YGGCCR 2 cut(s) 1165, 1190
AcsI RAATTY 2 cut(s) 52, 1722
AcuI CTGAAG 3 cut(s) 122, 636, 1677
AcyI GRCGYC 1 cut(s) 90
AfiI CCNNNNNNNGG 3 cut(s) 28, 237, 1692
AgeI ACCGGT 1 cut(s) 223
AgsI TTSAA 7 cut(s) 482, 564, 737, 788, 1177, 1349, 1497
AjnI CCWGG 6 cut(s) 83, 185, 236, 948, 1254, 1572
AluBI AGCT 4 cut(s) 148, 794, 1301, 1581
AluI AGCT 4 cut(s) 148, 794, 1301, 1581
Alw21I GWGCWC 2 cut(s) 150, 538
Alw26I GTCTC 3 cut(s) 232, 1221, 1586
Alw44I GTGCAC 1 cut(s) 534
AlwI GGATC 4 cut(s) 15, 410, 959, 1470
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 1 cut(s) 253
AoxI GGCC 8 cut(s) 189, 455, 775, 1041, 1165, 1190, 1483, 1781
ApaI GGGCCC 1 cut(s) 459
ApaLI GTGCAC 1 cut(s) 534
ApeKI GCWGC 7 cut(s) 161, 215, 442, 473, 741, 794, 1416
ApoI RAATTY 2 cut(s) 52, 1722
ArsI GACNNNNNNTTYG 2 cut(s) 1698, 1730
AseI ATTAAT 1 cut(s) 1505
AsiGI ACCGGT 1 cut(s) 223
Asp700I GAANNNNTTC 1 cut(s) 1353
AspA2I CCTAGG 1 cut(s) 1610
AspLEI GCGC 2 cut(s) 382, 1779
AspS9I GGNCC 6 cut(s) 19, 189, 455, 456, 775, 1782
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 213
AvaI CYCGRG 1 cut(s) 253
AvaII GGWCC 1 cut(s) 19
AvrII CCTAGG 1 cut(s) 1610
AxyI CCTNAGG 1 cut(s) 1367
BaeGI GKGCMC 3 cut(s) 273, 459, 538
BaeI ACNNNNGTAYC 2 cut(s) 1236, 1269
BalI TGGCCA 2 cut(s) 1167, 1192
BanI GGYRCC 1 cut(s) 268
BanII GRGCYC 4 cut(s) 69, 150, 254, 459
BauI CACGAG 1 cut(s) 930
Bbv12I GWGCWC 2 cut(s) 150, 538
BbvCI CCTCAGC 2 cut(s) 357, 1743
BbvI GCAGC 7 cut(s) 173, 202, 454, 485, 753, 781, 1428
BccI CCATC 4 cut(s) 337, 424, 1063, 1641
BceAI ACGGC 1 cut(s) 1028
BciT130I CCWGG 6 cut(s) 85, 187, 238, 950, 1256, 1574
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 3 cut(s) 232, 1221, 1586
BfaI CTAG 7 cut(s) 437, 524, 576, 860, 1611, 1689, 1818
BfmI CTRYAG 1 cut(s) 159
BfuAI ACCTGC 2 cut(s) 30, 1441
BglII AGATCT 1 cut(s) 1558
BisI GCNGC 9 cut(s) 59, 162, 216, 246, 443, 474, 742, 795, 1417
BlnI CCTAGG 1 cut(s) 1610
BlsI GCNGC 9 cut(s) 60, 163, 217, 247, 444, 475, 743, 796, 1418
Bme1390I CCNGG 7 cut(s) 85, 130, 187, 238, 950, 1256, 1574
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 253
BmgT120I GGNCC 6 cut(s) 19, 189, 455, 456, 775, 1782
BmiI GGNNCC 5 cut(s) 66, 270, 304, 457, 1784
BmrFI CCNGG 7 cut(s) 85, 130, 187, 238, 950, 1256, 1574
BmsI GCATC 7 cut(s) 172, 313, 556, 599, 610, 1297, 1801
BpmI CTGGAG 2 cut(s) 1581, 1605
Bpu10I CCTNAGC 2 cut(s) 357, 1743
BpuEI CTTGAG 2 cut(s) 791, 1472
BpuMI CCSGG 1 cut(s) 130
Bsa29I ATCGAT 1 cut(s) 428
BsaHI GRCGYC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 232
BsaJI CCNNGG 6 cut(s) 83, 84, 186, 366, 1113, 1610
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 708, 738
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 237, 1692
Bse118I RCCGGY 2 cut(s) 100, 223
Bse1I ACTGG 6 cut(s) 942, 1168, 1248, 1275, 1504, 1598
Bse21I CCTNAGG 1 cut(s) 1367
Bse3DI GCAATG 2 cut(s) 635, 899
BseBI CCWGG 6 cut(s) 85, 187, 238, 950, 1256, 1574
BseCI ATCGAT 1 cut(s) 428
BseDI CCNNGG 6 cut(s) 83, 84, 186, 366, 1113, 1610
BseGI GGATG 6 cut(s) 45, 328, 754, 967, 1264, 1816
BseLI CCNNNNNNNGG 3 cut(s) 28, 237, 1692
BseMI GCAATG 2 cut(s) 635, 899
BseMII CTCAG 3 cut(s) 26, 348, 1757
BseNI ACTGG 6 cut(s) 942, 1168, 1248, 1275, 1504, 1598
BseRI GAGGAG 6 cut(s) 29, 122, 184, 189, 198, 1731
BseSI GKGCMC 3 cut(s) 273, 459, 538
BseXI GCAGC 7 cut(s) 173, 202, 454, 485, 753, 781, 1428
BseYI CCCAGC 1 cut(s) 968
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 1777
BshFI GGCC 8 cut(s) 191, 457, 777, 1043, 1167, 1192, 1485, 1783
BshNI GGYRCC 1 cut(s) 268
BshTI ACCGGT 1 cut(s) 223
BshVI ATCGAT 1 cut(s) 428
BsiHKAI GWGCWC 2 cut(s) 150, 538
BsiHKCI CYCGRG 1 cut(s) 253
BsiSI CCGG 5 cut(s) 62, 101, 130, 212, 224
BslFI GGGAC 3 cut(s) 101, 1031, 1129
BslI CCNNNNNNNGG 3 cut(s) 28, 237, 1692
BsmAI GTCTC 3 cut(s) 232, 1221, 1586
BsmFI GGGAC 3 cut(s) 101, 1031, 1129
BsmI GAATGC 2 cut(s) 180, 372
BsnI GGCC 8 cut(s) 191, 457, 777, 1043, 1167, 1192, 1485, 1783
Bso31I GGTCTC 1 cut(s) 232
BsoBI CYCGRG 1 cut(s) 253
Bsp120I GGGCCC 1 cut(s) 455
Bsp1286I GDGCHC 6 cut(s) 69, 150, 254, 273, 459, 538
Bsp143I GATC 8 cut(s) 7, 415, 964, 1315, 1405, 1462, 1525, 1558
BspACI CCGC 8 cut(s) 5, 59, 73, 104, 245, 248, 689, 1035
BspANI GGCC 8 cut(s) 191, 457, 777, 1043, 1167, 1192, 1485, 1783
BspCNI CTCAG 3 cut(s) 27, 349, 1756
BspDI ATCGAT 1 cut(s) 428
BspFNI CGCG 1 cut(s) 1777
BspLI GGNNCC 5 cut(s) 66, 270, 304, 457, 1784
BspMAI CTGCAG 1 cut(s) 163
BspMI ACCTGC 2 cut(s) 30, 1441
BspPI GGATC 4 cut(s) 15, 410, 959, 1470
BspT107I GGYRCC 1 cut(s) 268
BspTNI GGTCTC 1 cut(s) 232
BsrDI GCAATG 2 cut(s) 635, 899
BsrFI RCCGGY 2 cut(s) 100, 223
BsrI ACTGG 6 cut(s) 942, 1168, 1248, 1275, 1504, 1598
BssAI RCCGGY 2 cut(s) 100, 223
BssECI CCNNGG 6 cut(s) 83, 84, 186, 366, 1113, 1610
BssMI GATC 8 cut(s) 7, 415, 964, 1315, 1405, 1462, 1525, 1558
BssNI GRCGYC 1 cut(s) 90
BssSI CACGAG 1 cut(s) 930
BssT1I CCWWGG 2 cut(s) 366, 1610
Bst2BI CACGAG 1 cut(s) 930
Bst2UI CCWGG 6 cut(s) 85, 187, 238, 950, 1256, 1574
Bst4CI ACNGT 3 cut(s) 1114, 1137, 1228
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 811
BstC8I GCNNGC 8 cut(s) 80, 102, 250, 1002, 1037, 1041, 1749, 1781
BstDEI CTNAG 7 cut(s) 35, 357, 641, 815, 1367, 1728, 1743
BstDSI CCRYGG 1 cut(s) 1113
BstF5I GGATG 6 cut(s) 45, 328, 754, 967, 1264, 1816
BstFNI CGCG 1 cut(s) 1777
BstHHI GCGC 2 cut(s) 382, 1779
BstKTI GATC 8 cut(s) 10, 418, 967, 1318, 1408, 1465, 1528, 1561
BstMAI GTCTC 3 cut(s) 232, 1221, 1586
BstMBI GATC 8 cut(s) 7, 415, 964, 1315, 1405, 1462, 1525, 1558
BstNI CCWGG 6 cut(s) 85, 187, 238, 950, 1256, 1574
BstNSI RCATGY 3 cut(s) 547, 1004, 1751
BstSCI CCNGG 7 cut(s) 83, 128, 185, 236, 948, 1254, 1572
BstSFI CTRYAG 1 cut(s) 159
BstSLI GKGCMC 3 cut(s) 273, 459, 538
BstUI CGCG 1 cut(s) 1777
BstV1I GCAGC 7 cut(s) 173, 202, 454, 485, 753, 781, 1428
BstX2I RGATCY 2 cut(s) 1462, 1558
BstXI CCANNNNNNTGG 2 cut(s) 1114, 1541
BstYI RGATCY 2 cut(s) 1462, 1558
Bsu15I ATCGAT 1 cut(s) 428
Bsu36I CCTNAGG 1 cut(s) 1367
BsuRI GGCC 8 cut(s) 191, 457, 777, 1043, 1167, 1192, 1485, 1783
BsuTUI ATCGAT 1 cut(s) 428
BtgI CCRYGG 1 cut(s) 1113
BtsCI GGATG 6 cut(s) 45, 328, 754, 967, 1264, 1816
BtsI GCAGTG 4 cut(s) 389, 873, 984, 1686
BtsIMutI CAGTG 8 cut(s) 32, 389, 873, 984, 1133, 1255, 1605, 1686
BveI ACCTGC 2 cut(s) 30, 1441
Cac8I GCNNGC 8 cut(s) 80, 102, 250, 1002, 1037, 1041, 1749, 1781
CaiI CAGNNNCTG 1 cut(s) 797
CfoI GCGC 2 cut(s) 382, 1779
Cfr10I RCCGGY 2 cut(s) 100, 223
Cfr13I GGNCC 6 cut(s) 19, 189, 455, 456, 775, 1782
ClaI ATCGAT 1 cut(s) 428
CseI GACGC 2 cut(s) 98, 844
CspAI ACCGGT 1 cut(s) 223
CviAII CATG 7 cut(s) 151, 446, 544, 669, 1001, 1477, 1748
DdeI CTNAG 7 cut(s) 35, 357, 641, 815, 1367, 1728, 1743
DpnI GATC 8 cut(s) 9, 417, 966, 1317, 1407, 1464, 1527, 1560
DpnII GATC 8 cut(s) 7, 415, 964, 1315, 1405, 1462, 1525, 1558
DraI TTTAAA 1 cut(s) 1095
EaeI YGGCCR 2 cut(s) 1165, 1190
EciI GGCGGA 2 cut(s) 20, 119
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 366, 1610
Eco147I AGGCCT 1 cut(s) 1485
Eco24I GRGCYC 4 cut(s) 69, 150, 254, 459
Eco31I GGTCTC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 3 cut(s) 122, 636, 1677
Eco81I CCTNAGG 1 cut(s) 1367
Eco88I CYCGRG 1 cut(s) 253
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 3 cut(s) 19, 456, 775
EcoRI GAATTC 1 cut(s) 52
EcoRII CCWGG 6 cut(s) 83, 185, 236, 948, 1254, 1572
EcoT14I CCWWGG 2 cut(s) 366, 1610
EcoT22I ATGCAT 1 cut(s) 549
EcoT38I GRGCYC 4 cut(s) 69, 150, 254, 459
ErhI CCWWGG 2 cut(s) 366, 1610
FaeI CATG 7 cut(s) 154, 449, 547, 672, 1004, 1480, 1751
FalI AAGNNNNNCTT 2 cut(s) 1505, 1537
FaqI GGGAC 3 cut(s) 101, 1031, 1129
FatI CATG 7 cut(s) 150, 445, 543, 668, 1000, 1476, 1747
FauI CCCGC 2 cut(s) 241, 1028
Fnu4HI GCNGC 9 cut(s) 59, 162, 216, 246, 443, 474, 742, 795, 1417
FokI GGATG 6 cut(s) 52, 335, 761, 974, 1271, 1823
FriOI GRGCYC 4 cut(s) 69, 150, 254, 459
Fsp4HI GCNGC 9 cut(s) 59, 162, 216, 246, 443, 474, 742, 795, 1417
FspBI CTAG 7 cut(s) 437, 524, 576, 860, 1611, 1689, 1818
FspI TGCGCA 1 cut(s) 381
GlaI GCGC 2 cut(s) 381, 1778
GluI GCNGC 9 cut(s) 59, 162, 216, 246, 443, 474, 742, 795, 1417
GsaI CCCAGC 1 cut(s) 972
GsuI CTGGAG 2 cut(s) 1581, 1605
HaeIII GGCC 8 cut(s) 191, 457, 777, 1043, 1167, 1192, 1485, 1783
HapII CCGG 5 cut(s) 62, 101, 130, 212, 224
HgaI GACGC 2 cut(s) 98, 844
HhaI GCGC 2 cut(s) 382, 1779
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 7 cut(s) 154, 449, 547, 672, 1004, 1480, 1751
Hin6I GCGC 2 cut(s) 380, 1777
HinP1I GCGC 2 cut(s) 380, 1777
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HindIII AAGCTT 1 cut(s) 1299
HpaI GTTAAC 1 cut(s) 823
HpaII CCGG 5 cut(s) 62, 101, 130, 212, 224
HphI GGTGA 1 cut(s) 213
Hpy166II GTNNAC 4 cut(s) 536, 823, 1021, 1762
Hpy188I TCNGA 8 cut(s) 118, 616, 884, 1296, 1320, 1558, 1563, 1829
Hpy188III TCNNGA 9 cut(s) 11, 124, 419, 930, 1107, 1241, 1466, 1584, 1655
Hpy8I GTNNAC 4 cut(s) 536, 823, 1021, 1762
Hpy99I CGWCG 3 cut(s) 118, 838, 1064
HpyAV CCTTC 7 cut(s) 443, 731, 1268, 1272, 1343, 1393, 1510
HpyCH4III ACNGT 3 cut(s) 1114, 1137, 1228
HpyCH4IV ACGT 1 cut(s) 723
HpyF3I CTNAG 7 cut(s) 35, 357, 641, 815, 1367, 1728, 1743
HpySE526I ACGT 1 cut(s) 723
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 7 cut(s) 154, 449, 547, 672, 1004, 1480, 1751
HspAI GCGC 2 cut(s) 380, 1777
KroI GCCGGC 1 cut(s) 100
KroNI GCCGGC 1 cut(s) 102
KspAI GTTAAC 1 cut(s) 823
Kzo9I GATC 8 cut(s) 7, 415, 964, 1315, 1405, 1462, 1525, 1558
LmnI GCTCC 6 cut(s) 64, 75, 202, 302, 994, 1243
Lsp1109I GCAGC 7 cut(s) 173, 202, 454, 485, 753, 781, 1428
LweI GCATC 7 cut(s) 172, 313, 556, 599, 610, 1297, 1801
MaeI CTAG 7 cut(s) 437, 524, 576, 860, 1611, 1689, 1818
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 2 cut(s) 982, 1714
MalI GATC 8 cut(s) 9, 417, 966, 1317, 1407, 1464, 1527, 1560
MboI GATC 8 cut(s) 7, 415, 964, 1315, 1405, 1462, 1525, 1558
MboII GAAGA 3 cut(s) 629, 1400, 1670
MfeI CAATTG 2 cut(s) 296, 1798
MflI RGATCY 2 cut(s) 1462, 1558
MhlI GDGCHC 6 cut(s) 69, 150, 254, 273, 459, 538
MlsI TGGCCA 2 cut(s) 1167, 1192
MluNI TGGCCA 2 cut(s) 1167, 1192
MlyI GAGTC 5 cut(s) 119, 1151, 1223, 1588, 1645
MmeI TCCRAC 4 cut(s) 96, 1102, 1119, 1186
Mox20I TGGCCA 2 cut(s) 1167, 1192
Mph1103I ATGCAT 1 cut(s) 549
MreI CGCCGGCG 1 cut(s) 100
MroNI GCCGGC 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 1353
MscI TGGCCA 2 cut(s) 1167, 1192
MseI TTAA 6 cut(s) 822, 944, 1094, 1149, 1505, 1539
MslI CAYNNNNRTG 2 cut(s) 450, 1539
Msp20I TGGCCA 2 cut(s) 1167, 1192
MspA1I CMGCKG 1 cut(s) 794
MspI CCGG 5 cut(s) 62, 101, 130, 212, 224
MspR9I CCNGG 7 cut(s) 85, 130, 187, 238, 950, 1256, 1574
MunI CAATTG 2 cut(s) 296, 1798
Mva1269I GAATGC 2 cut(s) 180, 372
MvaI CCWGG 6 cut(s) 85, 187, 238, 950, 1256, 1574
MvnI CGCG 1 cut(s) 1777
NaeI GCCGGC 1 cut(s) 102
NciI CCSGG 1 cut(s) 130
NdeII GATC 8 cut(s) 7, 415, 964, 1315, 1405, 1462, 1525, 1558
NgoMIV GCCGGC 1 cut(s) 100
NlaIII CATG 7 cut(s) 154, 449, 547, 672, 1004, 1480, 1751
NlaIV GGNNCC 5 cut(s) 66, 270, 304, 457, 1784
NmeAIII GCCGAG 2 cut(s) 190, 331
NmuCI GTSAC 2 cut(s) 982, 1714
NsbI TGCGCA 1 cut(s) 381
NsiI ATGCAT 1 cut(s) 549
NspI RCATGY 3 cut(s) 547, 1004, 1751
PaeI GCATGC 2 cut(s) 1004, 1751
PasI CCCWGGG 1 cut(s) 84
PceI AGGCCT 1 cut(s) 1485
PctI GAATGC 2 cut(s) 180, 372
PdiI GCCGGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 1353
PfeI GAWTC 8 cut(s) 95, 788, 871, 1125, 1283, 1291, 1493, 1700
PfoI TCCNGGA 1 cut(s) 1254
PinAI ACCGGT 1 cut(s) 223
PkrI GCNGC 9 cut(s) 60, 163, 217, 247, 444, 475, 743, 796, 1418
PleI GAGTC 5 cut(s) 118, 1150, 1222, 1588, 1645
PpsI GAGTC 5 cut(s) 118, 1150, 1222, 1588, 1645
PpuMI RGGWCCY 1 cut(s) 19
PshBI ATTAAT 1 cut(s) 1505
Psp124BI GAGCTC 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 6 cut(s) 83, 185, 236, 948, 1254, 1572
PspFI CCCAGC 1 cut(s) 968
PspGI CCWGG 6 cut(s) 83, 185, 236, 948, 1254, 1572
PspN4I GGNNCC 5 cut(s) 66, 270, 304, 457, 1784
PspOMI GGGCCC 1 cut(s) 455
PspPI GGNCC 6 cut(s) 19, 189, 455, 456, 775, 1782
PspPPI RGGWCCY 1 cut(s) 19
PstI CTGCAG 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 2 cut(s) 1462, 1558
PvuII CAGCTG 1 cut(s) 794
RseI CAYNNNNRTG 2 cut(s) 450, 1539
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 6 cut(s) 822, 944, 1094, 1149, 1505, 1539
SatI GCNGC 9 cut(s) 59, 162, 216, 246, 443, 474, 742, 795, 1417
Sau3AI GATC 8 cut(s) 7, 415, 964, 1315, 1405, 1462, 1525, 1558
Sau96I GGNCC 6 cut(s) 19, 189, 455, 456, 775, 1782
SchI GAGTC 5 cut(s) 119, 1151, 1223, 1588, 1645
ScrFI CCNGG 7 cut(s) 85, 130, 187, 238, 950, 1256, 1574
SduI GDGCHC 6 cut(s) 69, 150, 254, 273, 459, 538
SfaNI GCATC 7 cut(s) 172, 313, 556, 599, 610, 1297, 1801
SfcI CTRYAG 1 cut(s) 159
SgrAI CRCCGGYG 1 cut(s) 100
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 2 cut(s) 450, 1539
SmlI CTYRAG 2 cut(s) 770, 1487
SmoI CTYRAG 2 cut(s) 770, 1487
SphI GCATGC 2 cut(s) 1004, 1751
SseBI AGGCCT 1 cut(s) 1485
SsiI CCGC 8 cut(s) 5, 59, 73, 104, 245, 248, 689, 1035
SspMI CTAG 7 cut(s) 437, 524, 576, 860, 1611, 1689, 1818
SstI GAGCTC 1 cut(s) 150
StuI AGGCCT 1 cut(s) 1485
StyD4I CCNGG 7 cut(s) 83, 128, 185, 236, 948, 1254, 1572
StyI CCWWGG 2 cut(s) 366, 1610
TaaI ACNGT 3 cut(s) 1114, 1137, 1228
TaiI ACGT 1 cut(s) 726
TaqI TCGA 5 cut(s) 180, 341, 428, 593, 1059
TauI GCSGC 2 cut(s) 61, 248
TfiI GAWTC 8 cut(s) 95, 788, 871, 1125, 1283, 1291, 1493, 1700
Tru1I TTAA 6 cut(s) 822, 944, 1094, 1149, 1505, 1539
Tru9I TTAA 6 cut(s) 822, 944, 1094, 1149, 1505, 1539
TscAI CASTG 8 cut(s) 39, 389, 873, 991, 1140, 1255, 1605, 1686
TseFI GTSAC 2 cut(s) 982, 1714
TseI GCWGC 7 cut(s) 161, 215, 442, 473, 741, 794, 1416
Tsp45I GTSAC 2 cut(s) 982, 1714
TspDTI ATGAA 7 cut(s) 462, 696, 765, 1281, 1327, 1346, 1713
TspGWI ACGGA 3 cut(s) 220, 534, 1077
TspRI CASTG 8 cut(s) 39, 389, 873, 991, 1140, 1255, 1605, 1686
VneI GTGCAC 1 cut(s) 534
VpaK11BI GGWCC 1 cut(s) 19
VspI ATTAAT 1 cut(s) 1505
XapI RAATTY 2 cut(s) 52, 1722
XceI RCATGY 3 cut(s) 547, 1004, 1751
XcmI CCANNNNNNNNNTGG 1 cut(s) 1580
XmaJI CCTAGG 1 cut(s) 1610
XmnI GAANNNNTTC 1 cut(s) 1353
XspI CTAG 7 cut(s) 437, 524, 576, 860, 1611, 1689, 1818
Zsp2I ATGCAT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.