RchiOBHm_Chr6g0275661

DUF4205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012146
Physical Location & Seq
Forward (+)
37540455 .. 37550003
9549 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24731

Sequence Viewer

Length: 1836 bp
ATGGCGGATCAGGAGGAGGACCTGCAAATGGCACTGAGGATGAGTATGCAGAATTCGCCGCCGGAGCCCAAGCGGAGCAAGCCCAGGGACGCCGATTCGCCGGCGGAGGAGTCGTCGGAAGTCAAGACCCGGCGACTTCAGCGAGAGCTCATGGCTACTGCAGCCGAGAGGAGAATGCTCGATGCCAGGGCCAACGCTCCTCCTACTCCTCCGGCTGCCTCACCGGTCTCCGTTTCCAGGTCTGCGGCGGGCTCGGGCTCGGGTTCGGGTTTGGCGCCCAATGTGGTTAGGAAAGAGAGGCAATTGGGAGCGGAGTTGAGCGTGGGGGATGCCAAGCAGTTGTTTTCGATGGTGTTTGGGGCTGAGGTTTCCAAGGGCATTCTTGCGCAGTGGAGTAATCAGGGTATAAGGTTTAGCCCTGATCCAGAAACATCGATGGGTCTAGTGCAGCATGAAGGTGGGCCCTGTGGCGTTTTAGCAGCCATTCAAGCACTTGTTCTCAAATACCTCCTTTTCTACCCAGACGGACTAGGTAAAGGTGCACCAAACATGCATCACAATTTGGGTTCAAGGACACTTTCTAGTCATTGTGTTGCATCGACTAACTTTTCCTCACTTTCTGAAGATGCAAAAGCAAGAGCATTGCTTAGAAGTATGGGTGAGATATTGTTCTCATGTGGAAGTAATGAAAGGGCGGTGGTTGCAACTTTGAGTGTTATTGGAGGTGACGTTGAGAGGTTTGAAGGCAGCAAGGATGAAGTAATAGCAAAATCACTTGAGGGCCTTTCTATTGAATCAGCTGCTGACTTGCATAAAGTGCTAAGAGTTAACACATACACGACGCAAGAAAGTGCATTACGGGGGCTAGAAGCAGTGATTCCTGTTTTCCGAAGTCGTATGGGGGCATTGCTGTTCCTTATTTCTGCTTTGCTTTCACGAGGACTGGACTTAATCCAGGCTGATAGGGATGATCCCAGCCTACCTCTTGTCACTGCTCCATTTGGGCATGCCTCACAGGAAATTGTGAACTTACTGCTCTGCGGGCAGGCCGTCCCAAATGTTTTCGACGGAAAGATGGATTTAGGTGGTGGTATGTCTTTAAAGGGCATATCCAGAACCGTGGAAGTTGGATTCCTCACACTGTTGGAGTCCCTTAATTTCTGCAAAGTTGGCCAGTATTTGAAATGCCCCAAATGGCCAATTTGGGTAGTTGGAAGTGAGTCTCATTACACAGTTCTCTTTGCTCCTGATACCAGTGTCCAGGATGAGAATGAACTGGAAGGAAGGGAATCACAGATTCGGAAAGCTTTTGATGCACAAGATCAGAGTGGGGGTGGTGGTTTCATTAGTGTTGAAGGCTTTCATCAAGTCCTTAGGGAAACTAATGTAAAAGTTCCAAGTGAGAAGGTTGATCTTCTTTGCAGCACAGGGTTTATTGTATGGAGTGAGTTCTGGCAGGTCATTTTGGATCTGGACAAGAGCATGGGAGGCCTCAAGGATTCAACTGGATTAATGGGCAAGAAGGTCTTTGATCTTTACCATTTTAATGGAATTGCAAAATCAGATCTGAATGGAAGCCAGGTAAGCTCTGGAGGCGAGACTCCAGTGCAAAGACCTAGGCTCACAAAATTGAGGGTGTCAGTTCCTCCAAGATGGACTCCTGAAGAATATATGACAGATGTAGCAGTGTCCTCTAGTGGGAATGAATCCAAAAGTCAAGTCACAGAAATTTCTAAGCCAGAGCCTCCTCAGCATGCACCTTTGGTGGACTGCATAAGAACACGCTGGCCCCGTGCTGTTTGCAATTGGGTAGGGGATGCGCCTAGTATTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4205

Protein Analysis

611

Amino Acids

65.98

Weight (kDa)

5.73

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MINDY-3_4_CD PF13898 117 - 423 1.5e-57 Deubiquitinating enzyme MINDY-3/4, conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012146)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43690
fragaria_vesca FvH4_2g13910 FvH4_2g13910
malus_domestica MD05G1023700.v1.1 MD10G1024300.v1.1
prunus_persica Prupe.8G028200_v2.0.a1 Prupe.8G028200_v2.0.a1
pyrus_communis pycom05g01520 pycom10g01610
rosa_chinensis RchiOBHm_Chr6g0275661
rosa_laevigata RLG00000013449
rosa_multiflora Rmu_sc0011044.1_g000001
rosa_roxburghii Rroxscaffold_7G00193050
rosa_rugosa Rorug06G0095400
rosa_samantha Rh6AG207300 Rh6BG209900 Rh6CG213300 Rh6DG203000
rosa_wichuraiana Rw6G018070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 387
Acc36I ACCTGC 2 cut(s) 30, 1447
AccB1I GGYRCC 1 cut(s) 274
AccBSI CCGCTC 1 cut(s) 311
AciI CCGC 9 cut(s) 5, 59, 73, 104, 245, 248, 311, 695, 1041
AclWI GGATC 4 cut(s) 15, 416, 965, 1476
AcoI YGGCCR 2 cut(s) 1171, 1196
AcsI RAATTY 2 cut(s) 52, 1728
AcuI CTGAAG 3 cut(s) 122, 642, 1683
AcyI GRCGYC 2 cut(s) 90, 275
AfiI CCNNNNNNNGG 3 cut(s) 28, 237, 1698
AgeI ACCGGT 1 cut(s) 223
AgsI TTSAA 7 cut(s) 488, 570, 743, 794, 1183, 1355, 1503
AjnI CCWGG 6 cut(s) 83, 185, 236, 954, 1260, 1578
AluBI AGCT 4 cut(s) 148, 800, 1307, 1587
AluI AGCT 4 cut(s) 148, 800, 1307, 1587
Alw21I GWGCWC 2 cut(s) 150, 544
Alw26I GTCTC 3 cut(s) 232, 1227, 1592
Alw44I GTGCAC 1 cut(s) 540
AlwI GGATC 4 cut(s) 15, 416, 965, 1476
AlwNI CAGNNNCTG 1 cut(s) 803
Ama87I CYCGRG 2 cut(s) 253, 259
AoxI GGCC 8 cut(s) 189, 461, 781, 1047, 1171, 1196, 1489, 1787
ApaI GGGCCC 1 cut(s) 465
ApaLI GTGCAC 1 cut(s) 540
ApeKI GCWGC 7 cut(s) 161, 215, 448, 479, 747, 800, 1422
ApoI RAATTY 2 cut(s) 52, 1728
ArsI GACNNNNNNTTYG 2 cut(s) 1704, 1736
AseI ATTAAT 1 cut(s) 1511
AsiGI ACCGGT 1 cut(s) 223
Asp700I GAANNNNTTC 1 cut(s) 1359
AspA2I CCTAGG 1 cut(s) 1616
AspLEI GCGC 3 cut(s) 277, 388, 1822
AspS9I GGNCC 6 cut(s) 19, 189, 461, 462, 781, 1788
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 3 cut(s) 213, 671, 737
AvaI CYCGRG 2 cut(s) 253, 259
AvaII GGWCC 1 cut(s) 19
AvrII CCTAGG 1 cut(s) 1616
AxyI CCTNAGG 1 cut(s) 1373
BaeGI GKGCMC 2 cut(s) 465, 544
BaeI ACNNNNGTAYC 2 cut(s) 1242, 1275
BalI TGGCCA 2 cut(s) 1173, 1198
BanI GGYRCC 1 cut(s) 274
BanII GRGCYC 5 cut(s) 69, 150, 254, 260, 465
BauI CACGAG 1 cut(s) 936
Bbv12I GWGCWC 2 cut(s) 150, 544
BbvCI CCTCAGC 2 cut(s) 363, 1749
BbvI GCAGC 7 cut(s) 173, 202, 460, 491, 759, 787, 1434
BccI CCATC 4 cut(s) 343, 430, 1069, 1647
BceAI ACGGC 1 cut(s) 1034
BciT130I CCWGG 6 cut(s) 85, 187, 238, 956, 1262, 1580
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 3 cut(s) 232, 1227, 1592
BfaI CTAG 7 cut(s) 443, 530, 582, 866, 1617, 1695, 1824
BfmI CTRYAG 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 278
BfuAI ACCTGC 2 cut(s) 30, 1447
BglII AGATCT 1 cut(s) 1564
BisI GCNGC 9 cut(s) 59, 162, 216, 246, 449, 480, 748, 801, 1423
BlnI CCTAGG 1 cut(s) 1616
BlsI GCNGC 9 cut(s) 60, 163, 217, 247, 450, 481, 749, 802, 1424
Bme1390I CCNGG 7 cut(s) 85, 130, 187, 238, 956, 1262, 1580
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 2 cut(s) 253, 259
BmgT120I GGNCC 6 cut(s) 19, 189, 461, 462, 781, 1788
BmiI GGNNCC 4 cut(s) 66, 276, 463, 1790
BmrFI CCNGG 7 cut(s) 85, 130, 187, 238, 956, 1262, 1580
BmsI GCATC 7 cut(s) 172, 319, 562, 605, 616, 1303, 1807
BpmI CTGGAG 2 cut(s) 1587, 1611
Bpu10I CCTNAGC 2 cut(s) 363, 1749
BpuEI CTTGAG 2 cut(s) 797, 1478
BpuMI CCSGG 1 cut(s) 130
Bsa29I ATCGAT 1 cut(s) 434
BsaHI GRCGYC 2 cut(s) 90, 275
BsaI GGTCTC 1 cut(s) 232
BsaJI CCNNGG 6 cut(s) 83, 84, 186, 372, 1119, 1616
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 714, 744
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 237, 1698
Bse118I RCCGGY 2 cut(s) 100, 223
Bse1I ACTGG 6 cut(s) 948, 1174, 1254, 1281, 1510, 1604
Bse21I CCTNAGG 1 cut(s) 1373
Bse3DI GCAATG 2 cut(s) 641, 905
BseBI CCWGG 6 cut(s) 85, 187, 238, 956, 1262, 1580
BseCI ATCGAT 1 cut(s) 434
BseDI CCNNGG 6 cut(s) 83, 84, 186, 372, 1119, 1616
BseGI GGATG 6 cut(s) 45, 334, 760, 973, 1270, 1822
BseLI CCNNNNNNNGG 3 cut(s) 28, 237, 1698
BseMI GCAATG 2 cut(s) 641, 905
BseMII CTCAG 3 cut(s) 26, 354, 1763
BseNI ACTGG 6 cut(s) 948, 1174, 1254, 1281, 1510, 1604
BseRI GAGGAG 6 cut(s) 29, 122, 184, 189, 198, 1737
BseSI GKGCMC 2 cut(s) 465, 544
BseXI GCAGC 7 cut(s) 173, 202, 460, 491, 759, 787, 1434
BseYI CCCAGC 1 cut(s) 974
BsgI GTGCAG 1 cut(s) 467
BshFI GGCC 8 cut(s) 191, 463, 783, 1049, 1173, 1198, 1491, 1789
BshNI GGYRCC 1 cut(s) 274
BshTI ACCGGT 1 cut(s) 223
BshVI ATCGAT 1 cut(s) 434
BsiHKAI GWGCWC 2 cut(s) 150, 544
BsiHKCI CYCGRG 2 cut(s) 253, 259
BsiSI CCGG 5 cut(s) 62, 101, 130, 212, 224
BslFI GGGAC 3 cut(s) 101, 1037, 1135
BslI CCNNNNNNNGG 3 cut(s) 28, 237, 1698
BsmAI GTCTC 3 cut(s) 232, 1227, 1592
BsmFI GGGAC 3 cut(s) 101, 1037, 1135
BsmI GAATGC 2 cut(s) 180, 378
BsnI GGCC 8 cut(s) 191, 463, 783, 1049, 1173, 1198, 1491, 1789
Bso31I GGTCTC 1 cut(s) 232
BsoBI CYCGRG 2 cut(s) 253, 259
Bsp120I GGGCCC 1 cut(s) 461
Bsp1286I GDGCHC 6 cut(s) 69, 150, 254, 260, 465, 544
Bsp143I GATC 8 cut(s) 7, 421, 970, 1321, 1411, 1468, 1531, 1564
BspACI CCGC 9 cut(s) 5, 59, 73, 104, 245, 248, 311, 695, 1041
BspANI GGCC 8 cut(s) 191, 463, 783, 1049, 1173, 1198, 1491, 1789
BspCNI CTCAG 3 cut(s) 27, 355, 1762
BspDI ATCGAT 1 cut(s) 434
BspLI GGNNCC 4 cut(s) 66, 276, 463, 1790
BspMAI CTGCAG 1 cut(s) 163
BspMI ACCTGC 2 cut(s) 30, 1447
BspPI GGATC 4 cut(s) 15, 416, 965, 1476
BspT107I GGYRCC 1 cut(s) 274
BspTNI GGTCTC 1 cut(s) 232
BsrBI CCGCTC 1 cut(s) 311
BsrDI GCAATG 2 cut(s) 641, 905
BsrFI RCCGGY 2 cut(s) 100, 223
BsrI ACTGG 6 cut(s) 948, 1174, 1254, 1281, 1510, 1604
BssAI RCCGGY 2 cut(s) 100, 223
BssECI CCNNGG 6 cut(s) 83, 84, 186, 372, 1119, 1616
BssMI GATC 8 cut(s) 7, 421, 970, 1321, 1411, 1468, 1531, 1564
BssNI GRCGYC 2 cut(s) 90, 275
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 2 cut(s) 372, 1616
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 6 cut(s) 85, 187, 238, 956, 1262, 1580
Bst4CI ACNGT 3 cut(s) 1120, 1143, 1234
BstACI GRCGYC 2 cut(s) 90, 275
BstAPI GCANNNNNTGC 1 cut(s) 817
BstC8I GCNNGC 8 cut(s) 80, 102, 250, 1008, 1043, 1047, 1755, 1787
BstDEI CTNAG 7 cut(s) 35, 363, 647, 821, 1373, 1734, 1749
BstDSI CCRYGG 1 cut(s) 1119
BstF5I GGATG 6 cut(s) 45, 334, 760, 973, 1270, 1822
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 3 cut(s) 277, 388, 1822
BstKTI GATC 8 cut(s) 10, 424, 973, 1324, 1414, 1471, 1534, 1567
BstMAI GTCTC 3 cut(s) 232, 1227, 1592
BstMBI GATC 8 cut(s) 7, 421, 970, 1321, 1411, 1468, 1531, 1564
BstNI CCWGG 6 cut(s) 85, 187, 238, 956, 1262, 1580
BstNSI RCATGY 3 cut(s) 553, 1010, 1757
BstSCI CCNGG 7 cut(s) 83, 128, 185, 236, 954, 1260, 1578
BstSFI CTRYAG 1 cut(s) 159
BstSLI GKGCMC 2 cut(s) 465, 544
BstV1I GCAGC 7 cut(s) 173, 202, 460, 491, 759, 787, 1434
BstX2I RGATCY 2 cut(s) 1468, 1564
BstXI CCANNNNNNTGG 2 cut(s) 1120, 1547
BstYI RGATCY 2 cut(s) 1468, 1564
Bsu15I ATCGAT 1 cut(s) 434
Bsu36I CCTNAGG 1 cut(s) 1373
BsuRI GGCC 8 cut(s) 191, 463, 783, 1049, 1173, 1198, 1491, 1789
BsuTUI ATCGAT 1 cut(s) 434
BtgI CCRYGG 1 cut(s) 1119
BtsCI GGATG 6 cut(s) 45, 334, 760, 973, 1270, 1822
BtsI GCAGTG 4 cut(s) 395, 879, 990, 1692
BtsIMutI CAGTG 8 cut(s) 32, 395, 879, 990, 1139, 1261, 1611, 1692
BveI ACCTGC 2 cut(s) 30, 1447
Cac8I GCNNGC 8 cut(s) 80, 102, 250, 1008, 1043, 1047, 1755, 1787
CaiI CAGNNNCTG 1 cut(s) 803
CfoI GCGC 3 cut(s) 277, 388, 1822
Cfr10I RCCGGY 2 cut(s) 100, 223
Cfr13I GGNCC 6 cut(s) 19, 189, 461, 462, 781, 1788
ClaI ATCGAT 1 cut(s) 434
CseI GACGC 2 cut(s) 98, 850
CspAI ACCGGT 1 cut(s) 223
CviAII CATG 7 cut(s) 151, 452, 550, 675, 1007, 1483, 1754
DdeI CTNAG 7 cut(s) 35, 363, 647, 821, 1373, 1734, 1749
DinI GGCGCC 1 cut(s) 276
DpnI GATC 8 cut(s) 9, 423, 972, 1323, 1413, 1470, 1533, 1566
DpnII GATC 8 cut(s) 7, 421, 970, 1321, 1411, 1468, 1531, 1564
DraI TTTAAA 1 cut(s) 1101
EaeI YGGCCR 2 cut(s) 1171, 1196
EciI GGCGGA 2 cut(s) 20, 119
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 372, 1616
Eco147I AGGCCT 1 cut(s) 1491
Eco24I GRGCYC 5 cut(s) 69, 150, 254, 260, 465
Eco31I GGTCTC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 3 cut(s) 122, 642, 1683
Eco81I CCTNAGG 1 cut(s) 1373
Eco88I CYCGRG 2 cut(s) 253, 259
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 3 cut(s) 19, 462, 781
EcoRI GAATTC 1 cut(s) 52
EcoRII CCWGG 6 cut(s) 83, 185, 236, 954, 1260, 1578
EcoT14I CCWWGG 2 cut(s) 372, 1616
EcoT22I ATGCAT 1 cut(s) 555
EcoT38I GRGCYC 5 cut(s) 69, 150, 254, 260, 465
EgeI GGCGCC 1 cut(s) 276
EheI GGCGCC 1 cut(s) 276
ErhI CCWWGG 2 cut(s) 372, 1616
FaeI CATG 7 cut(s) 154, 455, 553, 678, 1010, 1486, 1757
FalI AAGNNNNNCTT 2 cut(s) 1511, 1543
FaqI GGGAC 3 cut(s) 101, 1037, 1135
FatI CATG 7 cut(s) 150, 451, 549, 674, 1006, 1482, 1753
FauI CCCGC 2 cut(s) 241, 1034
Fnu4HI GCNGC 9 cut(s) 59, 162, 216, 246, 449, 480, 748, 801, 1423
FokI GGATG 6 cut(s) 52, 341, 767, 980, 1277, 1829
FriOI GRGCYC 5 cut(s) 69, 150, 254, 260, 465
Fsp4HI GCNGC 9 cut(s) 59, 162, 216, 246, 449, 480, 748, 801, 1423
FspBI CTAG 7 cut(s) 443, 530, 582, 866, 1617, 1695, 1824
FspI TGCGCA 1 cut(s) 387
GlaI GCGC 3 cut(s) 276, 387, 1821
GluI GCNGC 9 cut(s) 59, 162, 216, 246, 449, 480, 748, 801, 1423
GsaI CCCAGC 1 cut(s) 978
GsuI CTGGAG 2 cut(s) 1587, 1611
HaeII RGCGCY 1 cut(s) 278
HaeIII GGCC 8 cut(s) 191, 463, 783, 1049, 1173, 1198, 1491, 1789
HapII CCGG 5 cut(s) 62, 101, 130, 212, 224
HgaI GACGC 2 cut(s) 98, 850
HhaI GCGC 3 cut(s) 277, 388, 1822
Hin1I GRCGYC 2 cut(s) 90, 275
Hin1II CATG 7 cut(s) 154, 455, 553, 678, 1010, 1486, 1757
Hin6I GCGC 3 cut(s) 275, 386, 1820
HinP1I GCGC 3 cut(s) 275, 386, 1820
HincII GTYRAC 1 cut(s) 829
HindII GTYRAC 1 cut(s) 829
HindIII AAGCTT 1 cut(s) 1305
HpaI GTTAAC 1 cut(s) 829
HpaII CCGG 5 cut(s) 62, 101, 130, 212, 224
HphI GGTGA 3 cut(s) 213, 671, 737
Hpy166II GTNNAC 4 cut(s) 542, 829, 1027, 1768
Hpy188I TCNGA 8 cut(s) 118, 622, 890, 1302, 1326, 1564, 1569, 1835
Hpy188III TCNNGA 9 cut(s) 11, 124, 425, 936, 1113, 1247, 1472, 1590, 1661
Hpy8I GTNNAC 4 cut(s) 542, 829, 1027, 1768
Hpy99I CGWCG 3 cut(s) 118, 844, 1070
HpyAV CCTTC 7 cut(s) 449, 737, 1274, 1278, 1349, 1399, 1516
HpyCH4III ACNGT 3 cut(s) 1120, 1143, 1234
HpyCH4IV ACGT 1 cut(s) 729
HpyF3I CTNAG 7 cut(s) 35, 363, 647, 821, 1373, 1734, 1749
HpySE526I ACGT 1 cut(s) 729
Hsp92I GRCGYC 2 cut(s) 90, 275
Hsp92II CATG 7 cut(s) 154, 455, 553, 678, 1010, 1486, 1757
HspAI GCGC 3 cut(s) 275, 386, 1820
KasI GGCGCC 1 cut(s) 274
KroI GCCGGC 1 cut(s) 100
KroNI GCCGGC 1 cut(s) 102
KspAI GTTAAC 1 cut(s) 829
Kzo9I GATC 8 cut(s) 7, 421, 970, 1321, 1411, 1468, 1531, 1564
LmnI GCTCC 6 cut(s) 64, 75, 202, 308, 1000, 1249
Lsp1109I GCAGC 7 cut(s) 173, 202, 460, 491, 759, 787, 1434
LweI GCATC 7 cut(s) 172, 319, 562, 605, 616, 1303, 1807
MaeI CTAG 7 cut(s) 443, 530, 582, 866, 1617, 1695, 1824
MaeII ACGT 1 cut(s) 729
MaeIII GTNAC 3 cut(s) 725, 988, 1720
MalI GATC 8 cut(s) 9, 423, 972, 1323, 1413, 1470, 1533, 1566
MbiI CCGCTC 1 cut(s) 311
MboI GATC 8 cut(s) 7, 421, 970, 1321, 1411, 1468, 1531, 1564
MboII GAAGA 3 cut(s) 635, 1406, 1676
MfeI CAATTG 2 cut(s) 302, 1804
MflI RGATCY 2 cut(s) 1468, 1564
MhlI GDGCHC 6 cut(s) 69, 150, 254, 260, 465, 544
MlsI TGGCCA 2 cut(s) 1173, 1198
MluNI TGGCCA 2 cut(s) 1173, 1198
Mly113I GGCGCC 1 cut(s) 275
MlyI GAGTC 5 cut(s) 119, 1157, 1229, 1594, 1651
MmeI TCCRAC 4 cut(s) 96, 1108, 1125, 1192
Mox20I TGGCCA 2 cut(s) 1173, 1198
Mph1103I ATGCAT 1 cut(s) 555
MreI CGCCGGCG 1 cut(s) 100
MroNI GCCGGC 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 1359
MscI TGGCCA 2 cut(s) 1173, 1198
MseI TTAA 6 cut(s) 828, 950, 1100, 1155, 1511, 1545
MslI CAYNNNNRTG 2 cut(s) 456, 1545
Msp20I TGGCCA 2 cut(s) 1173, 1198
MspA1I CMGCKG 1 cut(s) 800
MspI CCGG 5 cut(s) 62, 101, 130, 212, 224
MspR9I CCNGG 7 cut(s) 85, 130, 187, 238, 956, 1262, 1580
MunI CAATTG 2 cut(s) 302, 1804
Mva1269I GAATGC 2 cut(s) 180, 378
MvaI CCWGG 6 cut(s) 85, 187, 238, 956, 1262, 1580
NaeI GCCGGC 1 cut(s) 102
NarI GGCGCC 1 cut(s) 275
NciI CCSGG 1 cut(s) 130
NdeII GATC 8 cut(s) 7, 421, 970, 1321, 1411, 1468, 1531, 1564
NgoMIV GCCGGC 1 cut(s) 100
NlaIII CATG 7 cut(s) 154, 455, 553, 678, 1010, 1486, 1757
NlaIV GGNNCC 4 cut(s) 66, 276, 463, 1790
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 3 cut(s) 725, 988, 1720
NsbI TGCGCA 1 cut(s) 387
NsiI ATGCAT 1 cut(s) 555
NspI RCATGY 3 cut(s) 553, 1010, 1757
PaeI GCATGC 2 cut(s) 1010, 1757
PasI CCCWGGG 1 cut(s) 84
PceI AGGCCT 1 cut(s) 1491
PcsI WCGNNNNNNNCGW 1 cut(s) 1789
PctI GAATGC 2 cut(s) 180, 378
PdiI GCCGGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 1359
PfeI GAWTC 8 cut(s) 95, 794, 877, 1131, 1289, 1297, 1499, 1706
PfoI TCCNGGA 1 cut(s) 1260
PinAI ACCGGT 1 cut(s) 223
PkrI GCNGC 9 cut(s) 60, 163, 217, 247, 450, 481, 749, 802, 1424
PleI GAGTC 5 cut(s) 118, 1156, 1228, 1594, 1651
PluTI GGCGCC 1 cut(s) 278
PpsI GAGTC 5 cut(s) 118, 1156, 1228, 1594, 1651
PpuMI RGGWCCY 1 cut(s) 19
PshBI ATTAAT 1 cut(s) 1511
Psp124BI GAGCTC 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 6 cut(s) 83, 185, 236, 954, 1260, 1578
PspFI CCCAGC 1 cut(s) 974
PspGI CCWGG 6 cut(s) 83, 185, 236, 954, 1260, 1578
PspN4I GGNNCC 4 cut(s) 66, 276, 463, 1790
PspOMI GGGCCC 1 cut(s) 461
PspPI GGNCC 6 cut(s) 19, 189, 461, 462, 781, 1788
PspPPI RGGWCCY 1 cut(s) 19
PstI CTGCAG 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 2 cut(s) 1468, 1564
PvuII CAGCTG 1 cut(s) 800
RseI CAYNNNNRTG 2 cut(s) 456, 1545
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 6 cut(s) 828, 950, 1100, 1155, 1511, 1545
SatI GCNGC 9 cut(s) 59, 162, 216, 246, 449, 480, 748, 801, 1423
Sau3AI GATC 8 cut(s) 7, 421, 970, 1321, 1411, 1468, 1531, 1564
Sau96I GGNCC 6 cut(s) 19, 189, 461, 462, 781, 1788
SchI GAGTC 5 cut(s) 119, 1157, 1229, 1594, 1651
ScrFI CCNGG 7 cut(s) 85, 130, 187, 238, 956, 1262, 1580
SduI GDGCHC 6 cut(s) 69, 150, 254, 260, 465, 544
SfaNI GCATC 7 cut(s) 172, 319, 562, 605, 616, 1303, 1807
SfcI CTRYAG 1 cut(s) 159
SfoI GGCGCC 1 cut(s) 276
SgrAI CRCCGGYG 1 cut(s) 100
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 2 cut(s) 456, 1545
SmlI CTYRAG 2 cut(s) 776, 1493
SmoI CTYRAG 2 cut(s) 776, 1493
SphI GCATGC 2 cut(s) 1010, 1757
SseBI AGGCCT 1 cut(s) 1491
SsiI CCGC 9 cut(s) 5, 59, 73, 104, 245, 248, 311, 695, 1041
SspDI GGCGCC 1 cut(s) 274
SspMI CTAG 7 cut(s) 443, 530, 582, 866, 1617, 1695, 1824
SstI GAGCTC 1 cut(s) 150
StuI AGGCCT 1 cut(s) 1491
StyD4I CCNGG 7 cut(s) 83, 128, 185, 236, 954, 1260, 1578
StyI CCWWGG 2 cut(s) 372, 1616
TaaI ACNGT 3 cut(s) 1120, 1143, 1234
TaiI ACGT 1 cut(s) 732
TaqI TCGA 5 cut(s) 180, 347, 434, 599, 1065
TauI GCSGC 2 cut(s) 61, 248
TfiI GAWTC 8 cut(s) 95, 794, 877, 1131, 1289, 1297, 1499, 1706
Tru1I TTAA 6 cut(s) 828, 950, 1100, 1155, 1511, 1545
Tru9I TTAA 6 cut(s) 828, 950, 1100, 1155, 1511, 1545
TscAI CASTG 8 cut(s) 39, 395, 879, 997, 1146, 1261, 1611, 1692
TseFI GTSAC 3 cut(s) 725, 988, 1720
TseI GCWGC 7 cut(s) 161, 215, 448, 479, 747, 800, 1422
Tsp45I GTSAC 3 cut(s) 725, 988, 1720
TspDTI ATGAA 7 cut(s) 468, 702, 771, 1287, 1333, 1352, 1719
TspGWI ACGGA 3 cut(s) 220, 540, 1083
TspRI CASTG 8 cut(s) 39, 395, 879, 997, 1146, 1261, 1611, 1692
VneI GTGCAC 1 cut(s) 540
VpaK11BI GGWCC 1 cut(s) 19
VspI ATTAAT 1 cut(s) 1511
XapI RAATTY 2 cut(s) 52, 1728
XceI RCATGY 3 cut(s) 553, 1010, 1757
XcmI CCANNNNNNNNNTGG 1 cut(s) 1586
XmaJI CCTAGG 1 cut(s) 1616
XmnI GAANNNNTTC 1 cut(s) 1359
XspI CTAG 7 cut(s) 443, 530, 582, 866, 1617, 1695, 1824
Zsp2I ATGCAT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.