pycom05g01520

DUF4205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012146
Physical Location & Seq
Forward (+)
1840912 .. 1847675
6764 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01520.5

Sequence Viewer

Length: 1860 bp
ATGGCGAATCACGAGGAGGATGACCTCCGAATGGCACTACGGATGAGTATGCAGAATTCACCTCCGGATCCCAAGCGTAGCAAGCCCAGGGACGGACTCGCCGGAGCTCCGGCTGAAGAGCCGTCGGAAGTGAAGACCCGCAGACTTCAGCGAGAGCTGATGGCTGCTGCAGCTGAGAAGAGAATGCTGGTCGCCAAGAAGTCTTCGCCTTCGGCTTCACCTAGCAAGGTGTTGTCCGGGTCTGCAGGTTCCGGTTCGGATTTGGCGCCAAAGGTGGTGACGAAAGATAAGGAATTGAGTTTGGAGCAAGTGAATTTGGGAAAGGGGTTGAGCGAAGAGGATGCAAAGCAGCTGTTTTCGATGGTTTTCGGGGCTGAGGTTACCAAGGGCATTCTTGCGCAGTGGAGTAATCAGGGTATAAGGTTTAGCCCTGATCCAGAAACATCTATGGGTCTAGTGCAGCATGAAGGTGGGCCCTGTGGCGTTTTAGCAGCCATTCAAGCATTTGTTCTCAAATACCTACTTTTCTACCCAGCTGAATTAGGTAAAGTTGCACCAAACATGCATCAAAATACGGGTTCAAGGACACCGTCTAATAGTCAGTGTGTTGCAACAAATAATTTTGCCTCTCTAACTGAAGATGCAAAAGCAAGAGTACTTATTAGAAGTATGGGTGAGATATTGTTCTTATGTGGAAGCAACAAAAGGGCGGTGATTGCGACTTTGAGTGTTATTGGAGAAGAAACTGAGAGGTCTGAAGACAGACTGAATGATGAAGTAATAACCAAGTCACTTGAAGGTCTTTCAATCGAATCGGCTGCTGATTTGCATAAAGTGCTAAGAGTTAGCACATACACAACACAAGAAAGTGCATTTCAGAGGCTCCAAGCAGTGCTTCCTGCTTTTCAAAGTCGTATGGGGGCACTGCTGTTCCTTATTTCTGCTTTACTTTCTCGTGGACTGGACTTAGTTCAATCTGATAGGGACGACCCCAGCCTACCCCTTGTCACTGCTCCGTTTGGCCATGCCTCACAGGAAATTGTGAACTTATTGCTCTGCGGGCAGGCAGTCCCTAATGTTTTCGATGGAAAGATGGATTTGGGTGGTGGCATGTCTTTAAAAGGCATATCCAAAACTGTGGAAGTTGGATTCCTCACTCTGTTAGAATCCCTAAATTTCTGTAAGGTTGGTCAGTATTTGAAAAGTCCAAAATGGCCAATCTGGGTAGTTGGAAGCGAGTCTCACTATACAGTCCTTTTTTCTCTTGATACCACTGTCCAAGATGAGAATGAGCTGGAAGGAAGGGAATCACAGATTCGGAAAGCTTTTGACGCACAAGATCAGAGCGGCGGCGGTGGTTTTATTAGTGTAGAAGGCTTTCATCAAGTCCTTAGGGAAACTAACGTAAAACTTCCAACTGAGAAGGTTGACCTCCTCTGCAGCACAGGATTTATTGTATGGAGCGAATTCTGGCAGGTCATTTTGGATTTGGACAAGAGCTTAGGAGGCCTAAAGGATTCAACTGGATTGATGGGTAAGAAGGTGTTTGATCTTTACCATTTTAATGGAATTGCAAAATCTGACCTAAATGGAAGCCAGACAAGCTCGGGAGGCGAGATTCCAGTTCAAAGACCTAGGCTCACAAAATTGAGGGTGTCAGTACCTCCAAGGTGGACTCCTGAAGAATACATGACAGATGTGGTAGTGACCTCAGCTTCTAGTGGTAATGAATCTGGAGGGAAAGTCACAGAAATTGCTAAGCCGGAGCCTGCTCAGCATGCACCTCTGGTGGACTGCATAAGAACTCGCTGGCCCCGTGCTGTCTGCAATTGGGTAGGTGACCCGCCTAGCATAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4205

Protein Analysis

620

Amino Acids

67.16

Weight (kDa)

5.78

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012146)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43690
fragaria_vesca FvH4_2g13910 FvH4_2g13910
malus_domestica MD05G1023700.v1.1 MD10G1024300.v1.1
prunus_persica Prupe.8G028200_v2.0.a1 Prupe.8G028200_v2.0.a1
pyrus_communis pycom05g01520 pycom10g01610
rosa_chinensis RchiOBHm_Chr6g0275661
rosa_laevigata RLG00000013449
rosa_multiflora Rmu_sc0011044.1_g000001
rosa_roxburghii Rroxscaffold_7G00193050
rosa_rugosa Rorug06G0095400
rosa_samantha Rh6AG207300 Rh6BG209900 Rh6CG213300 Rh6DG203000
rosa_wichuraiana Rw6G018070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 399
Acc36I ACCTGC 2 cut(s) 236, 1465
AccB1I GGYRCC 1 cut(s) 265
AccBSI CCGCTC 1 cut(s) 1347
AccIII TCCGGA 1 cut(s) 64
AciI CCGC 7 cut(s) 139, 710, 1059, 1347, 1350, 1353, 1844
AclWI GGATC 3 cut(s) 62, 75, 428
AcoI YGGCCR 2 cut(s) 1021, 1214
AcsI RAATTY 4 cut(s) 55, 313, 1174, 1466
AcuI CTGAAG 5 cut(s) 131, 135, 657, 777, 1701
AcyI GRCGYC 1 cut(s) 266
AfaI GTAC 2 cut(s) 657, 1662
AfiI CCNNNNNNNGG 2 cut(s) 31, 92
AgsI TTSAA 9 cut(s) 500, 582, 797, 807, 908, 974, 1201, 1521, 1628
AjnI CCWGG 1 cut(s) 86
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 1 cut(s) 1245
AlwI GGATC 3 cut(s) 62, 75, 428
Ama87I CYCGRG 1 cut(s) 1606
Aor13HI TCCGGA 1 cut(s) 64
AoxI GGCC 5 cut(s) 473, 1021, 1214, 1507, 1811
ApaI GGGCCC 1 cut(s) 477
ApeKI GCWGC 8 cut(s) 164, 167, 170, 349, 460, 491, 818, 1440
ApoI RAATTY 4 cut(s) 55, 313, 1174, 1466
Asp700I GAANNNNTTC 1 cut(s) 1377
AspA2I CCTAGG 1 cut(s) 1634
AspLEI GCGC 2 cut(s) 268, 400
AspS9I GGNCC 3 cut(s) 473, 474, 1812
AsuC2I CCSGG 1 cut(s) 238
AsuHPI GGTGA 6 cut(s) 51, 210, 289, 686, 724, 1850
AvaI CYCGRG 1 cut(s) 1606
AvrII CCTAGG 1 cut(s) 1634
AxyI CCTNAGG 1 cut(s) 1391
BaeGI GKGCMC 2 cut(s) 477, 925
BaeI ACNNNNGTAYC 2 cut(s) 1260, 1293
BalI TGGCCA 2 cut(s) 1023, 1216
BamHI GGATCC 1 cut(s) 67
BanI GGYRCC 1 cut(s) 265
BanII GRGCYC 2 cut(s) 109, 477
BauI CACGAG 2 cut(s) 11, 954
BbsI GAAGAC 3 cut(s) 140, 195, 765
Bbv12I GWGCWC 1 cut(s) 109
BbvCI CCTCAGC 2 cut(s) 375, 1711
BbvI GCAGC 8 cut(s) 151, 154, 182, 361, 472, 503, 805, 1452
BccI CCATC 5 cut(s) 154, 355, 1079, 1087, 1525
BceAI ACGGC 1 cut(s) 106
BcgI CGANNNNNNTGC 4 cut(s) 323, 357, 800, 834
BciT130I CCWGG 1 cut(s) 88
BcnI CCSGG 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 1245
BfaI CTAG 5 cut(s) 222, 455, 1635, 1719, 1848
BfmI CTRYAG 3 cut(s) 168, 243, 1438
BfoI RGCGCY 1 cut(s) 269
BfuAI ACCTGC 2 cut(s) 236, 1465
BlnI CCTAGG 1 cut(s) 1634
BlpI GCTNAGC 2 cut(s) 1758, 1773
BmcAI AGTACT 1 cut(s) 657
Bme1390I CCNGG 2 cut(s) 88, 238
BmeT110I CYCGRG 1 cut(s) 1606
BmgT120I GGNCC 3 cut(s) 473, 474, 1812
BmiI GGNNCC 7 cut(s) 69, 250, 267, 475, 884, 1767, 1814
BmrFI CCNGG 2 cut(s) 88, 238
BmsI GCATC 3 cut(s) 331, 574, 631
BpiI GAAGAC 3 cut(s) 140, 195, 765
BpmI CTGGAG 1 cut(s) 1755
Bpu10I CCTNAGC 3 cut(s) 375, 1501, 1711
Bpu1102I GCTNAGC 2 cut(s) 1758, 1773
BpuMI CCSGG 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 266
BsaJI CCNNGG 5 cut(s) 86, 87, 384, 1634, 1667
BsaWI WCCGGW 2 cut(s) 64, 251
BsaXI ACNNNNNCTCC 2 cut(s) 1728, 1758
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 92
Bse1I ACTGG 3 cut(s) 966, 1528, 1622
Bse21I CCTNAGG 1 cut(s) 1391
BseAI TCCGGA 1 cut(s) 64
BseBI CCWGG 1 cut(s) 88
BseDI CCNNGG 5 cut(s) 86, 87, 384, 1634, 1667
BseGI GGATG 3 cut(s) 25, 48, 346
BseLI CCNNNNNNNGG 2 cut(s) 31, 92
BseMII CTCAG 6 cut(s) 165, 366, 738, 1410, 1725, 1787
BseNI ACTGG 3 cut(s) 966, 1528, 1622
BseRI GAGGAG 2 cut(s) 29, 1424
BseSI GKGCMC 2 cut(s) 477, 925
BseXI GCAGC 8 cut(s) 151, 154, 182, 361, 472, 503, 805, 1452
BseYI CCCAGC 2 cut(s) 532, 992
BsgI GTGCAG 1 cut(s) 479
BshFI GGCC 5 cut(s) 475, 1023, 1216, 1509, 1813
BshNI GGYRCC 1 cut(s) 265
BsiHKAI GWGCWC 1 cut(s) 109
BsiHKCI CYCGRG 1 cut(s) 1606
BsiSI CCGG 6 cut(s) 65, 102, 110, 237, 252, 1763
BslFI GGGAC 3 cut(s) 104, 998, 1055
BslI CCNNNNNNNGG 2 cut(s) 31, 92
BsmAI GTCTC 1 cut(s) 1245
BsmFI GGGAC 3 cut(s) 104, 998, 1055
BsmI GAATGC 2 cut(s) 189, 390
BsnI GGCC 5 cut(s) 475, 1023, 1216, 1509, 1813
BsoBI CYCGRG 1 cut(s) 1606
Bsp120I GGGCCC 1 cut(s) 473
Bsp1286I GDGCHC 3 cut(s) 109, 477, 925
Bsp13I TCCGGA 1 cut(s) 64
Bsp143I GATC 4 cut(s) 67, 433, 1339, 1549
Bsp1720I GCTNAGC 2 cut(s) 1758, 1773
BspACI CCGC 7 cut(s) 139, 710, 1059, 1347, 1350, 1353, 1844
BspANI GGCC 5 cut(s) 475, 1023, 1216, 1509, 1813
BspCNI CTCAG 6 cut(s) 166, 367, 739, 1411, 1724, 1786
BspEI TCCGGA 1 cut(s) 64
BspLI GGNNCC 7 cut(s) 69, 250, 267, 475, 884, 1767, 1814
BspMAI CTGCAG 3 cut(s) 172, 247, 1442
BspMI ACCTGC 2 cut(s) 236, 1465
BspPI GGATC 3 cut(s) 62, 75, 428
BspQI GCTCTTC 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 265
BsrBI CCGCTC 1 cut(s) 1347
BsrI ACTGG 3 cut(s) 966, 1528, 1622
BssECI CCNNGG 5 cut(s) 86, 87, 384, 1634, 1667
BssMI GATC 4 cut(s) 67, 433, 1339, 1549
BssNI GRCGYC 1 cut(s) 266
BssSI CACGAG 2 cut(s) 11, 954
BssT1I CCWWGG 3 cut(s) 384, 1634, 1667
Bst2BI CACGAG 2 cut(s) 11, 954
Bst2UI CCWGG 1 cut(s) 88
Bst4CI ACNGT 4 cut(s) 591, 1138, 1252, 1276
Bst6I CTCTTC 3 cut(s) 111, 173, 330
BstACI GRCGYC 1 cut(s) 266
BstAPI GCANNNNNTGC 1 cut(s) 835
BstC8I GCNNGC 6 cut(s) 83, 1061, 1065, 1770, 1779, 1811
BstEII GGTNACC 2 cut(s) 379, 1838
BstF5I GGATG 3 cut(s) 25, 48, 346
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 2 cut(s) 268, 400
BstKTI GATC 4 cut(s) 70, 436, 1342, 1552
BstMAI GTCTC 1 cut(s) 1245
BstMBI GATC 4 cut(s) 67, 433, 1339, 1549
BstNI CCWGG 1 cut(s) 88
BstNSI RCATGY 3 cut(s) 565, 1114, 1781
BstPI GGTNACC 2 cut(s) 379, 1838
BstSCI CCNGG 2 cut(s) 86, 236
BstSFI CTRYAG 3 cut(s) 168, 243, 1438
BstSLI GKGCMC 2 cut(s) 477, 925
BstV1I GCAGC 8 cut(s) 151, 154, 182, 361, 472, 503, 805, 1452
BstV2I GAAGAC 3 cut(s) 140, 195, 765
BstX2I RGATCY 1 cut(s) 67
BstXI CCANNNNNNTGG 2 cut(s) 1138, 1565
BstYI RGATCY 1 cut(s) 67
Bsu36I CCTNAGG 1 cut(s) 1391
BsuRI GGCC 5 cut(s) 475, 1023, 1216, 1509, 1813
BtsCI GGATG 3 cut(s) 25, 48, 346
BtsI GCAGTG 4 cut(s) 407, 897, 923, 1008
BtsIMutI CAGTG 6 cut(s) 407, 608, 897, 923, 1008, 1272
BveI ACCTGC 2 cut(s) 236, 1465
Cac8I GCNNGC 6 cut(s) 83, 1061, 1065, 1770, 1779, 1811
CfoI GCGC 2 cut(s) 268, 400
Cfr13I GGNCC 3 cut(s) 473, 474, 1812
CseI GACGC 1 cut(s) 1340
Csp6I GTAC 2 cut(s) 656, 1661
CviAII CATG 6 cut(s) 464, 562, 1025, 1111, 1690, 1778
CviQI GTAC 2 cut(s) 656, 1661
DinI GGCGCC 1 cut(s) 267
DpnI GATC 4 cut(s) 69, 435, 1341, 1551
DpnII GATC 4 cut(s) 67, 433, 1339, 1549
DraI TTTAAA 1 cut(s) 1119
EaeI YGGCCR 2 cut(s) 1021, 1214
Eam1104I CTCTTC 3 cut(s) 111, 173, 330
EarI CTCTTC 3 cut(s) 111, 173, 330
Ecl136II GAGCTC 1 cut(s) 107
Eco130I CCWWGG 3 cut(s) 384, 1634, 1667
Eco147I AGGCCT 1 cut(s) 1509
Eco24I GRGCYC 2 cut(s) 109, 477
Eco53kI GAGCTC 1 cut(s) 107
Eco57I CTGAAG 5 cut(s) 131, 135, 657, 777, 1701
Eco81I CCTNAGG 1 cut(s) 1391
Eco88I CYCGRG 1 cut(s) 1606
Eco91I GGTNACC 2 cut(s) 379, 1838
EcoICRI GAGCTC 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 474
EcoO65I GGTNACC 2 cut(s) 379, 1838
EcoRI GAATTC 2 cut(s) 55, 1466
EcoRII CCWGG 1 cut(s) 86
EcoT14I CCWWGG 3 cut(s) 384, 1634, 1667
EcoT22I ATGCAT 1 cut(s) 567
EcoT38I GRGCYC 2 cut(s) 109, 477
EgeI GGCGCC 1 cut(s) 267
EheI GGCGCC 1 cut(s) 267
ErhI CCWWGG 3 cut(s) 384, 1634, 1667
FaeI CATG 6 cut(s) 467, 565, 1028, 1114, 1693, 1781
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FaqI GGGAC 3 cut(s) 104, 998, 1055
FatI CATG 6 cut(s) 463, 561, 1024, 1110, 1689, 1777
FauI CCCGC 3 cut(s) 146, 1052, 1851
FokI GGATG 3 cut(s) 32, 55, 353
FriOI GRGCYC 2 cut(s) 109, 477
FspBI CTAG 5 cut(s) 222, 455, 1635, 1719, 1848
FspI TGCGCA 1 cut(s) 399
GlaI GCGC 2 cut(s) 267, 399
GsaI CCCAGC 2 cut(s) 536, 996
GsuI CTGGAG 1 cut(s) 1755
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 5 cut(s) 475, 1023, 1216, 1509, 1813
HapII CCGG 6 cut(s) 65, 102, 110, 237, 252, 1763
HgaI GACGC 1 cut(s) 1340
HhaI GCGC 2 cut(s) 268, 400
Hin1I GRCGYC 1 cut(s) 266
Hin1II CATG 6 cut(s) 467, 565, 1028, 1114, 1693, 1781
Hin6I GCGC 2 cut(s) 266, 398
HinP1I GCGC 2 cut(s) 266, 398
HincII GTYRAC 1 cut(s) 1429
HindII GTYRAC 1 cut(s) 1429
HindIII AAGCTT 1 cut(s) 1323
HpaII CCGG 6 cut(s) 65, 102, 110, 237, 252, 1763
HphI GGTGA 6 cut(s) 51, 210, 289, 686, 724, 1850
Hpy166II GTNNAC 5 cut(s) 959, 1045, 1429, 1674, 1792
Hpy188III TCNNGA 7 cut(s) 11, 65, 437, 1265, 1608, 1679, 1734
Hpy8I GTNNAC 5 cut(s) 959, 1045, 1429, 1674, 1792
Hpy99I CGWCG 1 cut(s) 127
HpyAV CCTTC 8 cut(s) 219, 461, 791, 1292, 1296, 1367, 1417, 1534
HpyCH4III ACNGT 4 cut(s) 591, 1138, 1252, 1276
HpyCH4IV ACGT 1 cut(s) 1404
HpySE526I ACGT 1 cut(s) 1404
Hsp92I GRCGYC 1 cut(s) 266
Hsp92II CATG 6 cut(s) 467, 565, 1028, 1114, 1693, 1781
HspAI GCGC 2 cut(s) 266, 398
KasI GGCGCC 1 cut(s) 265
Kpn2I TCCGGA 1 cut(s) 64
Kzo9I GATC 4 cut(s) 67, 433, 1339, 1549
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 7 cut(s) 104, 112, 304, 888, 1018, 1461, 1765
Lsp1109I GCAGC 8 cut(s) 151, 154, 182, 361, 472, 503, 805, 1452
LweI GCATC 3 cut(s) 331, 574, 631
MaeI CTAG 5 cut(s) 222, 455, 1635, 1719, 1848
MaeII ACGT 1 cut(s) 1404
MaeIII GTNAC 7 cut(s) 277, 379, 789, 1006, 1705, 1744, 1838
MalI GATC 4 cut(s) 69, 435, 1341, 1551
MbiI CCGCTC 1 cut(s) 1347
MboI GATC 4 cut(s) 67, 433, 1339, 1549
MboII GAAGA 9 cut(s) 128, 145, 190, 195, 347, 650, 752, 770, 1694
MfeI CAATTG 1 cut(s) 1828
MflI RGATCY 1 cut(s) 67
MhlI GDGCHC 3 cut(s) 109, 477, 925
MlsI TGGCCA 2 cut(s) 1023, 1216
MluNI TGGCCA 2 cut(s) 1023, 1216
Mly113I GGCGCC 1 cut(s) 266
MlyI GAGTC 3 cut(s) 90, 1247, 1669
MmeI TCCRAC 4 cut(s) 105, 1126, 1210, 1439
Mox20I TGGCCA 2 cut(s) 1023, 1216
Mph1103I ATGCAT 1 cut(s) 567
MroI TCCGGA 1 cut(s) 64
MroXI GAANNNNTTC 1 cut(s) 1377
MscI TGGCCA 2 cut(s) 1023, 1216
MseI TTAA 2 cut(s) 1118, 1563
MslI CAYNNNNRTG 2 cut(s) 468, 1563
Msp20I TGGCCA 2 cut(s) 1023, 1216
MspA1I CMGCKG 3 cut(s) 173, 352, 536
MspI CCGG 6 cut(s) 65, 102, 110, 237, 252, 1763
MspR9I CCNGG 2 cut(s) 88, 238
MunI CAATTG 1 cut(s) 1828
Mva1269I GAATGC 2 cut(s) 189, 390
MvaI CCWGG 1 cut(s) 88
NarI GGCGCC 1 cut(s) 266
NciI CCSGG 1 cut(s) 238
NdeII GATC 4 cut(s) 67, 433, 1339, 1549
NlaIII CATG 6 cut(s) 467, 565, 1028, 1114, 1693, 1781
NlaIV GGNNCC 7 cut(s) 69, 250, 267, 475, 884, 1767, 1814
NmuCI GTSAC 6 cut(s) 277, 789, 1006, 1705, 1744, 1838
NsbI TGCGCA 1 cut(s) 399
NsiI ATGCAT 1 cut(s) 567
NspI RCATGY 3 cut(s) 565, 1114, 1781
PaeI GCATGC 1 cut(s) 1781
PasI CCCWGGG 1 cut(s) 87
PceI AGGCCT 1 cut(s) 1509
PciSI GCTCTTC 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 1813
PctI GAATGC 2 cut(s) 189, 390
PdmI GAANNNNTTC 1 cut(s) 1377
PfeI GAWTC 9 cut(s) 7, 812, 1149, 1166, 1307, 1315, 1517, 1618, 1730
PflFI GACNNNGTC 1 cut(s) 589
PleI GAGTC 3 cut(s) 90, 1246, 1669
PluTI GGCGCC 1 cut(s) 269
PpsI GAGTC 3 cut(s) 90, 1246, 1669
Psp124BI GAGCTC 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 86
PspEI GGTNACC 2 cut(s) 379, 1838
PspFI CCCAGC 2 cut(s) 532, 992
PspGI CCWGG 1 cut(s) 86
PspN4I GGNNCC 7 cut(s) 69, 250, 267, 475, 884, 1767, 1814
PspOMI GGGCCC 1 cut(s) 473
PspPI GGNCC 3 cut(s) 473, 474, 1812
PstI CTGCAG 3 cut(s) 172, 247, 1442
PsuI RGATCY 1 cut(s) 67
PsyI GACNNNGTC 1 cut(s) 589
PvuII CAGCTG 3 cut(s) 173, 352, 536
RsaI GTAC 2 cut(s) 657, 1662
RsaNI GTAC 2 cut(s) 656, 1661
RseI CAYNNNNRTG 2 cut(s) 468, 1563
SacI GAGCTC 1 cut(s) 109
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 2 cut(s) 1118, 1563
Sau3AI GATC 4 cut(s) 67, 433, 1339, 1549
Sau96I GGNCC 3 cut(s) 473, 474, 1812
ScaI AGTACT 1 cut(s) 657
SchI GAGTC 3 cut(s) 90, 1247, 1669
ScrFI CCNGG 2 cut(s) 88, 238
SduI GDGCHC 3 cut(s) 109, 477, 925
SfaNI GCATC 3 cut(s) 331, 574, 631
SfcI CTRYAG 3 cut(s) 168, 243, 1438
SfoI GGCGCC 1 cut(s) 267
SmiMI CAYNNNNRTG 2 cut(s) 468, 1563
SphI GCATGC 1 cut(s) 1781
SseBI AGGCCT 1 cut(s) 1509
SsiI CCGC 7 cut(s) 139, 710, 1059, 1347, 1350, 1353, 1844
SspDI GGCGCC 1 cut(s) 265
SspMI CTAG 5 cut(s) 222, 455, 1635, 1719, 1848
SstI GAGCTC 1 cut(s) 109
StuI AGGCCT 1 cut(s) 1509
StyD4I CCNGG 2 cut(s) 86, 236
StyI CCWWGG 3 cut(s) 384, 1634, 1667
TaaI ACNGT 4 cut(s) 591, 1138, 1252, 1276
TaiI ACGT 1 cut(s) 1407
TaqI TCGA 3 cut(s) 359, 810, 1083
TatI WGTACW 1 cut(s) 655
TauI GCSGC 2 cut(s) 1350, 1353
TfiI GAWTC 9 cut(s) 7, 812, 1149, 1166, 1307, 1315, 1517, 1618, 1730
Tru1I TTAA 2 cut(s) 1118, 1563
Tru9I TTAA 2 cut(s) 1118, 1563
TscAI CASTG 6 cut(s) 407, 608, 897, 930, 1015, 1279
TseFI GTSAC 6 cut(s) 277, 789, 1006, 1705, 1744, 1838
TseI GCWGC 8 cut(s) 164, 167, 170, 349, 460, 491, 818, 1440
Tsp45I GTSAC 6 cut(s) 277, 789, 1006, 1705, 1744, 1838
TspDTI ATGAA 4 cut(s) 480, 789, 1370, 1743
TspGWI ACGGA 3 cut(s) 55, 108, 1005
TspRI CASTG 6 cut(s) 407, 608, 897, 930, 1015, 1279
Tth111I GACNNNGTC 1 cut(s) 589
XapI RAATTY 4 cut(s) 55, 313, 1174, 1466
XceI RCATGY 3 cut(s) 565, 1114, 1781
XmaJI CCTAGG 1 cut(s) 1634
XmnI GAANNNNTTC 1 cut(s) 1377
XspI CTAG 5 cut(s) 222, 455, 1635, 1719, 1848
ZrmI AGTACT 1 cut(s) 657
Zsp2I ATGCAT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.