Rh6BG209900

DUF4205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012146
Physical Location & Seq
Forward (+)
39585606 .. 39594822
9217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG209900.1

Sequence Viewer

Length: 1410 bp
ATGTGCAACCATATCATGATTGTGCATTTTCCATTCATCACAGTTGTTTGTGTGGTTGCGCAGGCACTTGTTCTCAAATACCTCCTTTTCTACCCAGACGGAGTAGGTAAAGGTGCACCAAACATGCATCACAATTTGGGTTCAAGGACACTTTCTAGTCATTGTGTTGCATCGACTAACTTTTCCTCACTTTCTGAAGATGCAAAAGCAAGAGCATTGCTTAGAAGTATGGGTGAGATATTGTTCTCATGTGGAAGTAATGAAAGGGCGGTGGTTGCAACTTTGAGTGTTATTGGAGATGACGTTGAGAGGTTTGAAGGCAGCAAGGATGAAGTAATAGCAAAATCACTTGAGGGCCTTTCTATTGAATCAGCTGCTGACTTGCATAAAGTGCTAAGAGTTAACACATACACGACGCAAGAAAGTGCATTACGGGGGCTAGAAGCAGTGATTCCTGTTTTCCGAAGTCGTATGGGGGCATTGCTGTTCCTTATTTCTGCTTTGCTTTCACGAGGACTGGACTTAATCCAGGCTGATAGGGATGATCCCAGCCTACCTCTTGTCACTGCTCCATTTGGGCATGCCTCACAGGAAATTGTGAACTTACTGCTCTGCGGGCAGGCCGTCCCAAATGTTTTCGACGGAAAGATGGATTTAGGTGGTGGTATGTCTTTAAAGGGCATATCCAGAACTGTGGAAGTTGGATTCCTCACACTGTTGGAGTCCCTTAATTTCTGCAAAGTTGGCCAGTATTTGAAATGCCCCAAATGGCCAATTTGGGTAGTTGGAAGTGAGTCTCATTATACAGTTCTCTTTGCTCCTGATACCAGTGTCCAGGATGAGAATGAACTGGAAGGAAGGGAATCACAGATTCGGAAAGCTTTTGATGCACAAGATCAGAGTGGGGGTGGTGGTTTCATTAGTGTTGAAGGCTTTCATCAAGTCCTTAGGGAAACTAATGTAAAAGTTCCAAGTGAGAAGGTTGATCTTCTTTGCAGCACAGGATTTATTGTATGGAGTGAGTTCTGGCAGGTCATTTTGGATCTGGACAAGAGCATGGGAGGCCTCAAGGATTCAACTGGATTAATGGGCAAGAAGGTCTTTGATCTTTACCATTTTAATGGAATTGCAAAATCAGATCTGAATGGAAGCCAGGTAAGCTCTGGAGGCGAGACTCCAGTGCAAAGACCTAGGCTCACAAAATTGAGGGTGTCAGTTCCTCCAAGATGGACTCCTGAAGAATATATGACAGATGTAGCAGTGTCCTCTAGTGGGAATGAATCCAAAAGTCAAGTCACAGAAATTTCTAAGCCAGAGCCTCCTCAGCATGCACCTTTGGTGGACTGCATAAGAACACGCTGGCCCCGTGCTGTTTGCAATTGGGTAGGGGATGCGCCTAGTATTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4205

Protein Analysis

469

Amino Acids

51.13

Weight (kDa)

5.72

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MINDY-3_4_CD PF13898 16 - 281 1.3e-44 Deubiquitinating enzyme MINDY-3/4, conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012146)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43690
fragaria_vesca FvH4_2g13910 FvH4_2g13910
malus_domestica MD05G1023700.v1.1 MD10G1024300.v1.1
prunus_persica Prupe.8G028200_v2.0.a1 Prupe.8G028200_v2.0.a1
pyrus_communis pycom05g01520 pycom10g01610
rosa_chinensis RchiOBHm_Chr6g0275661
rosa_laevigata RLG00000013449
rosa_multiflora Rmu_sc0011044.1_g000001
rosa_roxburghii Rroxscaffold_7G00193050
rosa_rugosa Rorug06G0095400
rosa_samantha Rh6AG207300 Rh6BG209900 Rh6CG213300 Rh6DG203000
rosa_wichuraiana Rw6G018070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 60
Acc36I ACCTGC 1 cut(s) 1021
AciI CCGC 2 cut(s) 269, 615
AclWI GGATC 2 cut(s) 539, 1050
AcoI YGGCCR 2 cut(s) 745, 770
AcsI RAATTY 1 cut(s) 1302
AcuI CTGAAG 2 cut(s) 216, 1257
AfiI CCNNNNNNNGG 1 cut(s) 1272
AgsI TTSAA 6 cut(s) 144, 317, 368, 757, 929, 1077
AjnI CCWGG 3 cut(s) 528, 834, 1152
AluBI AGCT 3 cut(s) 374, 881, 1161
AluI AGCT 3 cut(s) 374, 881, 1161
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 2 cut(s) 801, 1166
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 2 cut(s) 539, 1050
AlwNI CAGNNNCTG 1 cut(s) 377
AoxI GGCC 6 cut(s) 355, 621, 745, 770, 1063, 1361
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 3 cut(s) 321, 374, 996
ApoI RAATTY 1 cut(s) 1302
ArsI GACNNNNNNTTYG 2 cut(s) 1278, 1310
AseI ATTAAT 1 cut(s) 1085
Asp700I GAANNNNTTC 1 cut(s) 933
AspA2I CCTAGG 1 cut(s) 1190
AspLEI GCGC 2 cut(s) 61, 1396
AspS9I GGNCC 2 cut(s) 355, 1362
AsuHPI GGTGA 1 cut(s) 245
AvrII CCTAGG 1 cut(s) 1190
AxyI CCTNAGG 1 cut(s) 947
BaeGI GKGCMC 1 cut(s) 118
BaeI ACNNNNGTAYC 2 cut(s) 816, 849
BalI TGGCCA 2 cut(s) 747, 772
BauI CACGAG 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 118
BbvCI CCTCAGC 1 cut(s) 1323
BbvI GCAGC 3 cut(s) 333, 361, 1008
BccI CCATC 2 cut(s) 643, 1221
BceAI ACGGC 1 cut(s) 608
BciT130I CCWGG 3 cut(s) 530, 836, 1154
BcoDI GTCTC 2 cut(s) 801, 1166
BfaI CTAG 5 cut(s) 156, 440, 1191, 1269, 1398
BfuAI ACCTGC 1 cut(s) 1021
BglII AGATCT 1 cut(s) 1138
BisI GCNGC 3 cut(s) 322, 375, 997
BlnI CCTAGG 1 cut(s) 1190
BlsI GCNGC 3 cut(s) 323, 376, 998
Bme1390I CCNGG 3 cut(s) 530, 836, 1154
BmgT120I GGNCC 2 cut(s) 355, 1362
BmiI GGNNCC 1 cut(s) 1364
BmrFI CCNGG 3 cut(s) 530, 836, 1154
BmsI GCATC 5 cut(s) 136, 179, 190, 877, 1381
BpmI CTGGAG 2 cut(s) 1161, 1185
Bpu10I CCTNAGC 1 cut(s) 1323
BpuEI CTTGAG 2 cut(s) 371, 1052
BsaJI CCNNGG 1 cut(s) 1190
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 1 cut(s) 1272
Bse1I ACTGG 6 cut(s) 522, 748, 828, 855, 1084, 1178
Bse21I CCTNAGG 1 cut(s) 947
Bse3DI GCAATG 2 cut(s) 215, 479
BseBI CCWGG 3 cut(s) 530, 836, 1154
BseDI CCNNGG 1 cut(s) 1190
BseGI GGATG 4 cut(s) 334, 547, 844, 1396
BseLI CCNNNNNNNGG 1 cut(s) 1272
BseMI GCAATG 2 cut(s) 215, 479
BseMII CTCAG 1 cut(s) 1337
BseNI ACTGG 6 cut(s) 522, 748, 828, 855, 1084, 1178
BseRI GAGGAG 1 cut(s) 1311
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 3 cut(s) 333, 361, 1008
BseYI CCCAGC 1 cut(s) 548
BshFI GGCC 6 cut(s) 357, 623, 747, 772, 1065, 1363
BsiHKAI GWGCWC 1 cut(s) 118
BslFI GGGAC 2 cut(s) 611, 709
BslI CCNNNNNNNGG 1 cut(s) 1272
BsmAI GTCTC 2 cut(s) 801, 1166
BsmFI GGGAC 2 cut(s) 611, 709
BsnI GGCC 6 cut(s) 357, 623, 747, 772, 1065, 1363
Bsp1286I GDGCHC 1 cut(s) 118
Bsp143I GATC 6 cut(s) 544, 895, 985, 1042, 1105, 1138
BspACI CCGC 2 cut(s) 269, 615
BspANI GGCC 6 cut(s) 357, 623, 747, 772, 1065, 1363
BspCNI CTCAG 1 cut(s) 1336
BspHI TCATGA 1 cut(s) 15
BspLI GGNNCC 1 cut(s) 1364
BspMI ACCTGC 1 cut(s) 1021
BspPI GGATC 2 cut(s) 539, 1050
BsrDI GCAATG 2 cut(s) 215, 479
BsrI ACTGG 6 cut(s) 522, 748, 828, 855, 1084, 1178
BssECI CCNNGG 1 cut(s) 1190
BssMI GATC 6 cut(s) 544, 895, 985, 1042, 1105, 1138
BssSI CACGAG 1 cut(s) 510
BssT1I CCWWGG 1 cut(s) 1190
Bst2BI CACGAG 1 cut(s) 510
Bst2UI CCWGG 3 cut(s) 530, 836, 1154
Bst4CI ACNGT 4 cut(s) 43, 694, 717, 808
BstAPI GCANNNNNTGC 1 cut(s) 391
BstC8I GCNNGC 6 cut(s) 63, 582, 617, 621, 1329, 1361
BstDEI CTNAG 5 cut(s) 221, 395, 947, 1308, 1323
BstF5I GGATG 4 cut(s) 334, 547, 844, 1396
BstHHI GCGC 2 cut(s) 61, 1396
BstKTI GATC 6 cut(s) 547, 898, 988, 1045, 1108, 1141
BstMAI GTCTC 2 cut(s) 801, 1166
BstMBI GATC 6 cut(s) 544, 895, 985, 1042, 1105, 1138
BstMWI GCNNNNNNNGC 9 cut(s) 275, 391, 616, 744, 887, 1062, 1158, 1167, 1324
BstNI CCWGG 3 cut(s) 530, 836, 1154
BstNSI RCATGY 3 cut(s) 127, 584, 1331
BstSCI CCNGG 3 cut(s) 528, 834, 1152
BstSLI GKGCMC 1 cut(s) 118
BstV1I GCAGC 3 cut(s) 333, 361, 1008
BstX2I RGATCY 2 cut(s) 1042, 1138
BstXI CCANNNNNNTGG 2 cut(s) 694, 1121
BstYI RGATCY 2 cut(s) 1042, 1138
Bsu36I CCTNAGG 1 cut(s) 947
BsuRI GGCC 6 cut(s) 357, 623, 747, 772, 1065, 1363
BtsCI GGATG 4 cut(s) 334, 547, 844, 1396
BtsI GCAGTG 3 cut(s) 453, 564, 1266
BtsIMutI CAGTG 6 cut(s) 453, 564, 713, 835, 1185, 1266
BveI ACCTGC 1 cut(s) 1021
Cac8I GCNNGC 6 cut(s) 63, 582, 617, 621, 1329, 1361
CaiI CAGNNNCTG 1 cut(s) 377
CciI TCATGA 1 cut(s) 15
CfoI GCGC 2 cut(s) 61, 1396
Cfr13I GGNCC 2 cut(s) 355, 1362
CseI GACGC 1 cut(s) 424
CviAII CATG 6 cut(s) 16, 124, 249, 581, 1057, 1328
DdeI CTNAG 5 cut(s) 221, 395, 947, 1308, 1323
DpnI GATC 6 cut(s) 546, 897, 987, 1044, 1107, 1140
DpnII GATC 6 cut(s) 544, 895, 985, 1042, 1105, 1138
DraI TTTAAA 1 cut(s) 675
EaeI YGGCCR 2 cut(s) 745, 770
Eco130I CCWWGG 1 cut(s) 1190
Eco147I AGGCCT 1 cut(s) 1065
Eco57I CTGAAG 2 cut(s) 216, 1257
Eco81I CCTNAGG 1 cut(s) 947
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 3 cut(s) 528, 834, 1152
EcoT14I CCWWGG 1 cut(s) 1190
EcoT22I ATGCAT 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 1190
FaeI CATG 6 cut(s) 19, 127, 252, 584, 1060, 1331
FalI AAGNNNNNCTT 2 cut(s) 1085, 1117
FaqI GGGAC 2 cut(s) 611, 709
FatI CATG 6 cut(s) 15, 123, 248, 580, 1056, 1327
FauI CCCGC 1 cut(s) 608
Fnu4HI GCNGC 3 cut(s) 322, 375, 997
FokI GGATG 4 cut(s) 341, 554, 851, 1403
Fsp4HI GCNGC 3 cut(s) 322, 375, 997
FspBI CTAG 5 cut(s) 156, 440, 1191, 1269, 1398
FspI TGCGCA 1 cut(s) 60
GlaI GCGC 2 cut(s) 60, 1395
GluI GCNGC 3 cut(s) 322, 375, 997
GsaI CCCAGC 1 cut(s) 552
GsuI CTGGAG 2 cut(s) 1161, 1185
HaeIII GGCC 6 cut(s) 357, 623, 747, 772, 1065, 1363
HgaI GACGC 1 cut(s) 424
HhaI GCGC 2 cut(s) 61, 1396
Hin1II CATG 6 cut(s) 19, 127, 252, 584, 1060, 1331
Hin6I GCGC 2 cut(s) 59, 1394
HinP1I GCGC 2 cut(s) 59, 1394
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 1 cut(s) 879
HpaI GTTAAC 1 cut(s) 403
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 4 cut(s) 116, 403, 601, 1342
Hpy188I TCNGA 7 cut(s) 196, 464, 876, 900, 1138, 1143, 1409
Hpy188III TCNNGA 7 cut(s) 16, 510, 687, 821, 1046, 1164, 1235
Hpy8I GTNNAC 4 cut(s) 116, 403, 601, 1342
Hpy99I CGWCG 2 cut(s) 418, 644
HpyAV CCTTC 6 cut(s) 311, 848, 852, 923, 973, 1090
HpyCH4III ACNGT 4 cut(s) 43, 694, 717, 808
HpyCH4IV ACGT 1 cut(s) 303
HpyF10VI GCNNNNNNNGC 9 cut(s) 275, 391, 616, 744, 887, 1062, 1158, 1167, 1324
HpyF3I CTNAG 5 cut(s) 221, 395, 947, 1308, 1323
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 6 cut(s) 19, 127, 252, 584, 1060, 1331
HspAI GCGC 2 cut(s) 59, 1394
KspAI GTTAAC 1 cut(s) 403
Kzo9I GATC 6 cut(s) 544, 895, 985, 1042, 1105, 1138
LmnI GCTCC 2 cut(s) 574, 823
Lsp1109I GCAGC 3 cut(s) 333, 361, 1008
LweI GCATC 5 cut(s) 136, 179, 190, 877, 1381
MaeI CTAG 5 cut(s) 156, 440, 1191, 1269, 1398
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 2 cut(s) 562, 1294
MalI GATC 6 cut(s) 546, 897, 987, 1044, 1107, 1140
MboI GATC 6 cut(s) 544, 895, 985, 1042, 1105, 1138
MboII GAAGA 3 cut(s) 209, 980, 1250
MfeI CAATTG 1 cut(s) 1378
MflI RGATCY 2 cut(s) 1042, 1138
MhlI GDGCHC 1 cut(s) 118
MlsI TGGCCA 2 cut(s) 747, 772
MluCI AATT 8 cut(s) 133, 594, 730, 774, 1125, 1202, 1302, 1378
MluNI TGGCCA 2 cut(s) 747, 772
MlyI GAGTC 4 cut(s) 731, 803, 1168, 1225
MmeI TCCRAC 3 cut(s) 682, 699, 766
Mox20I TGGCCA 2 cut(s) 747, 772
Mph1103I ATGCAT 1 cut(s) 129
MroXI GAANNNNTTC 1 cut(s) 933
MscI TGGCCA 2 cut(s) 747, 772
MseI TTAA 6 cut(s) 402, 524, 674, 729, 1085, 1119
MslI CAYNNNNRTG 2 cut(s) 20, 1119
Msp20I TGGCCA 2 cut(s) 747, 772
MspA1I CMGCKG 1 cut(s) 374
MspR9I CCNGG 3 cut(s) 530, 836, 1154
MunI CAATTG 1 cut(s) 1378
MvaI CCWGG 3 cut(s) 530, 836, 1154
MwoI GCNNNNNNNGC 9 cut(s) 275, 391, 616, 744, 887, 1062, 1158, 1167, 1324
NdeII GATC 6 cut(s) 544, 895, 985, 1042, 1105, 1138
NlaIII CATG 6 cut(s) 19, 127, 252, 584, 1060, 1331
NlaIV GGNNCC 1 cut(s) 1364
NmuCI GTSAC 2 cut(s) 562, 1294
NsbI TGCGCA 1 cut(s) 60
NsiI ATGCAT 1 cut(s) 129
NspI RCATGY 3 cut(s) 127, 584, 1331
PaeI GCATGC 2 cut(s) 584, 1331
PagI TCATGA 1 cut(s) 15
PceI AGGCCT 1 cut(s) 1065
PcsI WCGNNNNNNNCGW 1 cut(s) 1363
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 7 cut(s) 368, 451, 705, 863, 871, 1073, 1280
PfoI TCCNGGA 1 cut(s) 834
PkrI GCNGC 3 cut(s) 323, 376, 998
PleI GAGTC 4 cut(s) 730, 802, 1168, 1225
PpsI GAGTC 4 cut(s) 730, 802, 1168, 1225
PshBI ATTAAT 1 cut(s) 1085
Psp6I CCWGG 3 cut(s) 528, 834, 1152
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 3 cut(s) 528, 834, 1152
PspN4I GGNNCC 1 cut(s) 1364
PspPI GGNCC 2 cut(s) 355, 1362
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 2 cut(s) 1042, 1138
PvuII CAGCTG 1 cut(s) 374
RseI CAYNNNNRTG 2 cut(s) 20, 1119
SaqAI TTAA 6 cut(s) 402, 524, 674, 729, 1085, 1119
SatI GCNGC 3 cut(s) 322, 375, 997
Sau3AI GATC 6 cut(s) 544, 895, 985, 1042, 1105, 1138
Sau96I GGNCC 2 cut(s) 355, 1362
SchI GAGTC 4 cut(s) 731, 803, 1168, 1225
ScrFI CCNGG 3 cut(s) 530, 836, 1154
SduI GDGCHC 1 cut(s) 118
SfaNI GCATC 5 cut(s) 136, 179, 190, 877, 1381
SmiMI CAYNNNNRTG 2 cut(s) 20, 1119
SmlI CTYRAG 2 cut(s) 350, 1067
SmoI CTYRAG 2 cut(s) 350, 1067
SphI GCATGC 2 cut(s) 584, 1331
Sse9I AATT 8 cut(s) 133, 594, 730, 774, 1125, 1202, 1302, 1378
SseBI AGGCCT 1 cut(s) 1065
SsiI CCGC 2 cut(s) 269, 615
SspMI CTAG 5 cut(s) 156, 440, 1191, 1269, 1398
StuI AGGCCT 1 cut(s) 1065
StyD4I CCNGG 3 cut(s) 528, 834, 1152
StyI CCWWGG 1 cut(s) 1190
TaaI ACNGT 4 cut(s) 43, 694, 717, 808
TaiI ACGT 1 cut(s) 306
TaqI TCGA 2 cut(s) 173, 639
TasI AATT 8 cut(s) 133, 594, 730, 774, 1125, 1202, 1302, 1378
TfiI GAWTC 7 cut(s) 368, 451, 705, 863, 871, 1073, 1280
Tru1I TTAA 6 cut(s) 402, 524, 674, 729, 1085, 1119
Tru9I TTAA 6 cut(s) 402, 524, 674, 729, 1085, 1119
TscAI CASTG 6 cut(s) 453, 571, 720, 835, 1185, 1266
TseFI GTSAC 2 cut(s) 562, 1294
TseI GCWGC 3 cut(s) 321, 374, 996
Tsp45I GTSAC 2 cut(s) 562, 1294
TspDTI ATGAA 7 cut(s) 25, 276, 345, 861, 907, 926, 1293
TspGWI ACGGA 2 cut(s) 114, 657
TspRI CASTG 6 cut(s) 453, 571, 720, 835, 1185, 1266
VneI GTGCAC 1 cut(s) 114
VspI ATTAAT 1 cut(s) 1085
XapI RAATTY 1 cut(s) 1302
XceI RCATGY 3 cut(s) 127, 584, 1331
XcmI CCANNNNNNNNNTGG 1 cut(s) 1160
XmaJI CCTAGG 1 cut(s) 1190
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 5 cut(s) 156, 440, 1191, 1269, 1398
Zsp2I ATGCAT 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.