Rroxscaffold_7G00193050

DUF4205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012146
Physical Location & Seq
Reverse (-)
34418765 .. 34427370
8606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00193050.1

Sequence Viewer

Length: 1911 bp
ATGGCGGATCAGGAGGAGGACCTGCAAATGGCACTGAGGATGAGTATGCAGAATTCGCCGCCGGAGCCCAAGCGGAGCAAGCCCAGGGACGCCGATTCGCCGGCGGAGGAGTCGTCGGAAGTCAAGACCCGGCGACTTCAGCGAGAGCTCATGGCTAATGCAGCCGAGAGGAGAATGCTCGACGCCAGGGCCAACGCTCCTCCTACTCCTCCGGCTGCCTCGCCGGTCTCCGTTTCCAGGTCTGCGGCGGGCTCGGGTTCGGGTTCGGGTTCGGCGCCCAATGTGGTTATGAAAGAGAGGCAATTGGGAGCGGAGTTGAGCGTGGCGGATGCCAAGCAGTTGTTTTCGATGGTGTTTGGGGCTGAGGTTTCCAAGGGCATTCTTGCGCAGTGGAGTAATCAGGGTATAAGGTTTAGCCCTGATCCAGAAACATCGATGGGTCTAGTGCAGCATGAAGGTGGGCCCTGTGGCGTTTTAGCAGCCATTCAACATGATATTGACATGTGCAACCATATCATGATTGTGCATTTTGCATTCATCACAGTTGTTTGTGTGGTTGCGCAGGCACTTGTTCTCAAATACCTCCTTTTCTACCCAGACGGACTAGGTAAAGGTGCACCAAACATGCATCACAATTTGGGTTCAAGGACACTTTCTAGTCATTGTGTTGCATCGACTAACTTTTCCTCAATTTCTGAAGATGCAAAAGCAAGAGCATTGCTTAGAAGTATGGGTGAGATATTGTTCTCTTGTGGAAGTAATAAAAGGGTGGTGGTTGCAACTTTGAGTGTTATTGGAGATGATGTTGAGAGGTTTGAAGGCAGCAAGGATGAATTAATAGCAAAATCACTTGAGGGCCTTTCTATTGAATCAGCTGCAGACTTGCATAAAGTGCTAAGAGTTAACACATACACGACGCAAGAAAGTGCACTACGGGGGCTAGAAGCAGTGATTCCTGTTTTCCGAAGTCGTATGGGGGCTTTGCTGTTCCTTATTTCTGCTTTGCTTTCACGAGGACTGGACTTAATCCAGGCTGATAGGGATGATCCCAGCCTACCTCTTGTCACTGCTCCATTTGGGCATGCCTCACAGGAAATTGTGAACTTACTGCTCTGCGGGCAGGCCGTCCCAAATGTTTTTGATGGAAAGATGGATTTAGGTGGTGGTATGTCTTTAAAGGGCATATCCAGAACCGTGGAAGTTGGATTCCTCACACTGTTGGAGTCCCTTAATTTCTGCAAAGTTGGCCAGTATTTGAAATGCCCCAAATGGCCAATTTGGGTAGTTGGAAGTGAGTCTCATTATACAGTTCTCTTTGCTCCTGATACCAGTGTCCAGGATGAGAATGAACTGGAAGGAAGGGAATCACAGATTCGGAAAGCTTTTGATGCACAAGATCAGAGTGGGGGTGGTGGTTTCATTAGTGTTGAAGGCTTTCATCAAGTCCTTAGGGAAACTAATGTAAAAGTTCCAAGTGAGAACGTTGATCTTCTTTGCAGCACAGGATTTATTGTATGGAGTGAGTTCTGGCAGGTCATTTTGGATCTGGACAAGAGCATGGGAGGCCTCAAGGATTCAACTGGATTAATGGGCAAGAAGGTCTTTGATCTTTACCATTTTAATGGAATTGCAAAATCAGATCTGAATGGAAGCCAGGTAAGCTCAGGAGGCGAGACTCCAGTGCAAAGACCTAGGCTCACAAAATTGAGGGTGTCAGTTCCTCCAAGATGGACTCCTGAAGAATATATGACAGATGTAGCAGTGTCCTCTAGTGGGAATGAATCCAAAGGTCAAGTCACAGAAATTTCTAAGCCAGAGCCTCCTCAGCATGCACCTTTGGTGGACTGCATAAGAACGCGCTGGCCCCGTGCTGTTTGCAATTGGGTAGGGGATGCGCCTAGTATTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4205

Protein Analysis

636

Amino Acids

68.85

Weight (kDa)

5.65

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MINDY-3_4_CD PF13898 117 - 163 3.5e-06 Deubiquitinating enzyme MINDY-3/4, conserved domain
MINDY-3_4_CD PF13898 183 - 448 6.4e-44 Deubiquitinating enzyme MINDY-3/4, conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012146)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43690
fragaria_vesca FvH4_2g13910 FvH4_2g13910
malus_domestica MD05G1023700.v1.1 MD10G1024300.v1.1
prunus_persica Prupe.8G028200_v2.0.a1 Prupe.8G028200_v2.0.a1
pyrus_communis pycom05g01520 pycom10g01610
rosa_chinensis RchiOBHm_Chr6g0275661
rosa_laevigata RLG00000013449
rosa_multiflora Rmu_sc0011044.1_g000001
rosa_roxburghii Rroxscaffold_7G00193050
rosa_rugosa Rorug06G0095400
rosa_samantha Rh6AG207300 Rh6BG209900 Rh6CG213300 Rh6DG203000
rosa_wichuraiana Rw6G018070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 387, 561
Acc36I ACCTGC 2 cut(s) 30, 1522
AccB1I GGYRCC 1 cut(s) 274
AccBSI CCGCTC 1 cut(s) 311
AccII CGCG 1 cut(s) 1858
AciI CCGC 9 cut(s) 5, 59, 73, 104, 245, 248, 311, 326, 1116
AclI AACGTT 1 cut(s) 1482
AclWI GGATC 4 cut(s) 15, 416, 1040, 1551
AcoI YGGCCR 2 cut(s) 1246, 1271
AcsI RAATTY 2 cut(s) 52, 1803
AcuI CTGAAG 3 cut(s) 122, 717, 1758
AcyI GRCGYC 3 cut(s) 90, 183, 275
AfiI CCNNNNNNNGG 3 cut(s) 28, 237, 1773
AflIII ACRYGT 1 cut(s) 501
AgsI TTSAA 7 cut(s) 488, 645, 818, 869, 1258, 1430, 1578
AjnI CCWGG 6 cut(s) 83, 185, 236, 1029, 1335, 1653
AluBI AGCT 4 cut(s) 148, 875, 1382, 1662
AluI AGCT 4 cut(s) 148, 875, 1382, 1662
Alw21I GWGCWC 3 cut(s) 150, 619, 931
Alw26I GTCTC 3 cut(s) 232, 1302, 1667
Alw44I GTGCAC 2 cut(s) 615, 927
AlwI GGATC 4 cut(s) 15, 416, 1040, 1551
Ama87I CYCGRG 1 cut(s) 253
AoxI GGCC 8 cut(s) 189, 461, 856, 1122, 1246, 1271, 1564, 1862
ApaI GGGCCC 1 cut(s) 465
ApaLI GTGCAC 2 cut(s) 615, 927
ApeKI GCWGC 7 cut(s) 161, 215, 448, 479, 822, 875, 1497
ApoI RAATTY 2 cut(s) 52, 1803
ArsI GACNNNNNNTTYG 2 cut(s) 1779, 1811
AseI ATTAAT 2 cut(s) 836, 1586
Asp700I GAANNNNTTC 1 cut(s) 1434
AspA2I CCTAGG 1 cut(s) 1691
AspLEI GCGC 5 cut(s) 277, 388, 562, 1860, 1897
AspS9I GGNCC 6 cut(s) 19, 189, 461, 462, 856, 1863
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 746
AvaI CYCGRG 1 cut(s) 253
AvaII GGWCC 1 cut(s) 19
AvrII CCTAGG 1 cut(s) 1691
AxyI CCTNAGG 1 cut(s) 1448
BaeGI GKGCMC 3 cut(s) 465, 619, 931
BaeI ACNNNNGTAYC 2 cut(s) 1317, 1350
BalI TGGCCA 2 cut(s) 1248, 1273
BanI GGYRCC 1 cut(s) 274
BanII GRGCYC 4 cut(s) 69, 150, 254, 465
BauI CACGAG 1 cut(s) 1011
Bbv12I GWGCWC 3 cut(s) 150, 619, 931
BbvCI CCTCAGC 2 cut(s) 363, 1824
BbvI GCAGC 7 cut(s) 173, 202, 460, 491, 834, 862, 1509
BccI CCATC 5 cut(s) 343, 430, 1136, 1144, 1722
BceAI ACGGC 1 cut(s) 1109
BciT130I CCWGG 6 cut(s) 85, 187, 238, 1031, 1337, 1655
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 3 cut(s) 232, 1302, 1667
BfaI CTAG 7 cut(s) 443, 605, 657, 941, 1692, 1770, 1899
BfmI CTRYAG 1 cut(s) 876
BfoI RGCGCY 1 cut(s) 278
BfuAI ACCTGC 2 cut(s) 30, 1522
BglII AGATCT 1 cut(s) 1639
BisI GCNGC 9 cut(s) 59, 162, 216, 246, 449, 480, 823, 876, 1498
BlnI CCTAGG 1 cut(s) 1691
BlsI GCNGC 9 cut(s) 60, 163, 217, 247, 450, 481, 824, 877, 1499
Bme1390I CCNGG 7 cut(s) 85, 130, 187, 238, 1031, 1337, 1655
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 253
BmgT120I GGNCC 6 cut(s) 19, 189, 461, 462, 856, 1863
BmiI GGNNCC 4 cut(s) 66, 276, 463, 1865
BmrFI CCNGG 7 cut(s) 85, 130, 187, 238, 1031, 1337, 1655
BmsI GCATC 6 cut(s) 319, 637, 680, 691, 1378, 1882
BpmI CTGGAG 1 cut(s) 1662
Bpu10I CCTNAGC 3 cut(s) 363, 1663, 1824
BpuEI CTTGAG 2 cut(s) 872, 1553
BpuMI CCSGG 1 cut(s) 130
Bsa29I ATCGAT 1 cut(s) 434
BsaHI GRCGYC 3 cut(s) 90, 183, 275
BsaI GGTCTC 1 cut(s) 232
BsaJI CCNNGG 6 cut(s) 83, 84, 186, 372, 1194, 1691
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 789, 819
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 237, 1773
Bse118I RCCGGY 2 cut(s) 100, 223
Bse1I ACTGG 6 cut(s) 1023, 1249, 1329, 1356, 1585, 1679
Bse21I CCTNAGG 1 cut(s) 1448
Bse3DI GCAATG 1 cut(s) 716
BseBI CCWGG 6 cut(s) 85, 187, 238, 1031, 1337, 1655
BseCI ATCGAT 1 cut(s) 434
BseDI CCNNGG 6 cut(s) 83, 84, 186, 372, 1194, 1691
BseGI GGATG 6 cut(s) 45, 334, 835, 1048, 1345, 1897
BseLI CCNNNNNNNGG 3 cut(s) 28, 237, 1773
BseMI GCAATG 1 cut(s) 716
BseMII CTCAG 4 cut(s) 26, 354, 1677, 1838
BseNI ACTGG 6 cut(s) 1023, 1249, 1329, 1356, 1585, 1679
BseRI GAGGAG 6 cut(s) 29, 122, 184, 189, 198, 1812
BseSI GKGCMC 3 cut(s) 465, 619, 931
BseXI GCAGC 7 cut(s) 173, 202, 460, 491, 834, 862, 1509
BseYI CCCAGC 1 cut(s) 1049
BsgI GTGCAG 1 cut(s) 467
Bsh1236I CGCG 1 cut(s) 1858
BshFI GGCC 8 cut(s) 191, 463, 858, 1124, 1248, 1273, 1566, 1864
BshNI GGYRCC 1 cut(s) 274
BshVI ATCGAT 1 cut(s) 434
BsiHKAI GWGCWC 3 cut(s) 150, 619, 931
BsiHKCI CYCGRG 1 cut(s) 253
BsiSI CCGG 5 cut(s) 62, 101, 130, 212, 224
BslFI GGGAC 3 cut(s) 101, 1112, 1210
BslI CCNNNNNNNGG 3 cut(s) 28, 237, 1773
BsmAI GTCTC 3 cut(s) 232, 1302, 1667
BsmFI GGGAC 3 cut(s) 101, 1112, 1210
BsmI GAATGC 3 cut(s) 180, 378, 533
BsnI GGCC 8 cut(s) 191, 463, 858, 1124, 1248, 1273, 1566, 1864
Bso31I GGTCTC 1 cut(s) 232
BsoBI CYCGRG 1 cut(s) 253
Bsp120I GGGCCC 1 cut(s) 461
Bsp1286I GDGCHC 6 cut(s) 69, 150, 254, 465, 619, 931
Bsp143I GATC 8 cut(s) 7, 421, 1045, 1396, 1486, 1543, 1606, 1639
BspACI CCGC 9 cut(s) 5, 59, 73, 104, 245, 248, 311, 326, 1116
BspANI GGCC 8 cut(s) 191, 463, 858, 1124, 1248, 1273, 1566, 1864
BspCNI CTCAG 4 cut(s) 27, 355, 1676, 1837
BspDI ATCGAT 1 cut(s) 434
BspFNI CGCG 1 cut(s) 1858
BspHI TCATGA 1 cut(s) 516
BspLI GGNNCC 4 cut(s) 66, 276, 463, 1865
BspMAI CTGCAG 1 cut(s) 880
BspMI ACCTGC 2 cut(s) 30, 1522
BspPI GGATC 4 cut(s) 15, 416, 1040, 1551
BspT107I GGYRCC 1 cut(s) 274
BspTNI GGTCTC 1 cut(s) 232
BsrBI CCGCTC 1 cut(s) 311
BsrDI GCAATG 1 cut(s) 716
BsrFI RCCGGY 2 cut(s) 100, 223
BsrI ACTGG 6 cut(s) 1023, 1249, 1329, 1356, 1585, 1679
BssAI RCCGGY 2 cut(s) 100, 223
BssECI CCNNGG 6 cut(s) 83, 84, 186, 372, 1194, 1691
BssMI GATC 8 cut(s) 7, 421, 1045, 1396, 1486, 1543, 1606, 1639
BssNI GRCGYC 3 cut(s) 90, 183, 275
BssSI CACGAG 1 cut(s) 1011
BssT1I CCWWGG 2 cut(s) 372, 1691
Bst2BI CACGAG 1 cut(s) 1011
Bst2UI CCWGG 6 cut(s) 85, 187, 238, 1031, 1337, 1655
Bst4CI ACNGT 4 cut(s) 544, 1195, 1218, 1309
BstACI GRCGYC 3 cut(s) 90, 183, 275
BstAPI GCANNNNNTGC 1 cut(s) 892
BstC8I GCNNGC 9 cut(s) 80, 102, 250, 564, 1083, 1118, 1122, 1830, 1862
BstDEI CTNAG 8 cut(s) 35, 363, 722, 896, 1448, 1663, 1809, 1824
BstDSI CCRYGG 1 cut(s) 1194
BstF5I GGATG 6 cut(s) 45, 334, 835, 1048, 1345, 1897
BstFNI CGCG 1 cut(s) 1858
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 5 cut(s) 277, 388, 562, 1860, 1897
BstKTI GATC 8 cut(s) 10, 424, 1048, 1399, 1489, 1546, 1609, 1642
BstMAI GTCTC 3 cut(s) 232, 1302, 1667
BstMBI GATC 8 cut(s) 7, 421, 1045, 1396, 1486, 1543, 1606, 1639
BstNI CCWGG 6 cut(s) 85, 187, 238, 1031, 1337, 1655
BstNSI RCATGY 4 cut(s) 505, 628, 1085, 1832
BstSCI CCNGG 7 cut(s) 83, 128, 185, 236, 1029, 1335, 1653
BstSFI CTRYAG 1 cut(s) 876
BstSLI GKGCMC 3 cut(s) 465, 619, 931
BstUI CGCG 1 cut(s) 1858
BstV1I GCAGC 7 cut(s) 173, 202, 460, 491, 834, 862, 1509
BstX2I RGATCY 2 cut(s) 1543, 1639
BstXI CCANNNNNNTGG 2 cut(s) 1195, 1622
BstYI RGATCY 2 cut(s) 1543, 1639
Bsu15I ATCGAT 1 cut(s) 434
Bsu36I CCTNAGG 1 cut(s) 1448
BsuRI GGCC 8 cut(s) 191, 463, 858, 1124, 1248, 1273, 1566, 1864
BsuTUI ATCGAT 1 cut(s) 434
BtgI CCRYGG 1 cut(s) 1194
BtsCI GGATG 6 cut(s) 45, 334, 835, 1048, 1345, 1897
BtsI GCAGTG 4 cut(s) 395, 954, 1065, 1767
BtsIMutI CAGTG 8 cut(s) 32, 395, 954, 1065, 1214, 1336, 1686, 1767
BveI ACCTGC 2 cut(s) 30, 1522
Cac8I GCNNGC 9 cut(s) 80, 102, 250, 564, 1083, 1118, 1122, 1830, 1862
CciI TCATGA 1 cut(s) 516
CfoI GCGC 5 cut(s) 277, 388, 562, 1860, 1897
Cfr10I RCCGGY 2 cut(s) 100, 223
Cfr13I GGNCC 6 cut(s) 19, 189, 461, 462, 856, 1863
ClaI ATCGAT 1 cut(s) 434
CseI GACGC 3 cut(s) 98, 191, 925
CviAII CATG 9 cut(s) 151, 452, 491, 502, 517, 625, 1082, 1558, 1829
DdeI CTNAG 8 cut(s) 35, 363, 722, 896, 1448, 1663, 1809, 1824
DinI GGCGCC 1 cut(s) 276
DpnI GATC 8 cut(s) 9, 423, 1047, 1398, 1488, 1545, 1608, 1641
DpnII GATC 8 cut(s) 7, 421, 1045, 1396, 1486, 1543, 1606, 1639
DraI TTTAAA 1 cut(s) 1176
EaeI YGGCCR 2 cut(s) 1246, 1271
EciI GGCGGA 3 cut(s) 20, 119, 341
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 372, 1691
Eco147I AGGCCT 1 cut(s) 1566
Eco24I GRGCYC 4 cut(s) 69, 150, 254, 465
Eco31I GGTCTC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 3 cut(s) 122, 717, 1758
Eco81I CCTNAGG 1 cut(s) 1448
Eco88I CYCGRG 1 cut(s) 253
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 3 cut(s) 19, 462, 856
EcoRI GAATTC 1 cut(s) 52
EcoRII CCWGG 6 cut(s) 83, 185, 236, 1029, 1335, 1653
EcoT14I CCWWGG 2 cut(s) 372, 1691
EcoT22I ATGCAT 1 cut(s) 630
EcoT38I GRGCYC 4 cut(s) 69, 150, 254, 465
EgeI GGCGCC 1 cut(s) 276
EheI GGCGCC 1 cut(s) 276
ErhI CCWWGG 2 cut(s) 372, 1691
FaeI CATG 9 cut(s) 154, 455, 494, 505, 520, 628, 1085, 1561, 1832
FalI AAGNNNNNCTT 2 cut(s) 1586, 1618
FaqI GGGAC 3 cut(s) 101, 1112, 1210
FatI CATG 9 cut(s) 150, 451, 490, 501, 516, 624, 1081, 1557, 1828
FauI CCCGC 2 cut(s) 241, 1109
Fnu4HI GCNGC 9 cut(s) 59, 162, 216, 246, 449, 480, 823, 876, 1498
FokI GGATG 6 cut(s) 52, 341, 842, 1055, 1352, 1904
FriOI GRGCYC 4 cut(s) 69, 150, 254, 465
Fsp4HI GCNGC 9 cut(s) 59, 162, 216, 246, 449, 480, 823, 876, 1498
FspBI CTAG 7 cut(s) 443, 605, 657, 941, 1692, 1770, 1899
FspI TGCGCA 2 cut(s) 387, 561
GlaI GCGC 5 cut(s) 276, 387, 561, 1859, 1896
GluI GCNGC 9 cut(s) 59, 162, 216, 246, 449, 480, 823, 876, 1498
GsaI CCCAGC 1 cut(s) 1053
GsuI CTGGAG 1 cut(s) 1662
HaeII RGCGCY 1 cut(s) 278
HaeIII GGCC 8 cut(s) 191, 463, 858, 1124, 1248, 1273, 1566, 1864
HapII CCGG 5 cut(s) 62, 101, 130, 212, 224
HgaI GACGC 3 cut(s) 98, 191, 925
HhaI GCGC 5 cut(s) 277, 388, 562, 1860, 1897
Hin1I GRCGYC 3 cut(s) 90, 183, 275
Hin1II CATG 9 cut(s) 154, 455, 494, 505, 520, 628, 1085, 1561, 1832
Hin6I GCGC 5 cut(s) 275, 386, 560, 1858, 1895
HinP1I GCGC 5 cut(s) 275, 386, 560, 1858, 1895
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HindIII AAGCTT 1 cut(s) 1380
HpaI GTTAAC 1 cut(s) 904
HpaII CCGG 5 cut(s) 62, 101, 130, 212, 224
HphI GGTGA 1 cut(s) 746
Hpy166II GTNNAC 5 cut(s) 617, 904, 929, 1102, 1843
Hpy188I TCNGA 8 cut(s) 118, 697, 965, 1377, 1401, 1639, 1644, 1910
Hpy8I GTNNAC 5 cut(s) 617, 904, 929, 1102, 1843
Hpy99I CGWCG 3 cut(s) 118, 185, 919
HpyAV CCTTC 6 cut(s) 449, 812, 1349, 1353, 1424, 1591
HpyCH4III ACNGT 4 cut(s) 544, 1195, 1218, 1309
HpyCH4IV ACGT 1 cut(s) 1482
HpyF3I CTNAG 8 cut(s) 35, 363, 722, 896, 1448, 1663, 1809, 1824
HpySE526I ACGT 1 cut(s) 1482
Hsp92I GRCGYC 3 cut(s) 90, 183, 275
Hsp92II CATG 9 cut(s) 154, 455, 494, 505, 520, 628, 1085, 1561, 1832
HspAI GCGC 5 cut(s) 275, 386, 560, 1858, 1895
KasI GGCGCC 1 cut(s) 274
KroI GCCGGC 1 cut(s) 100
KroNI GCCGGC 1 cut(s) 102
KspAI GTTAAC 1 cut(s) 904
Kzo9I GATC 8 cut(s) 7, 421, 1045, 1396, 1486, 1543, 1606, 1639
LmnI GCTCC 6 cut(s) 64, 75, 202, 308, 1075, 1324
Lsp1109I GCAGC 7 cut(s) 173, 202, 460, 491, 834, 862, 1509
LweI GCATC 6 cut(s) 319, 637, 680, 691, 1378, 1882
MaeI CTAG 7 cut(s) 443, 605, 657, 941, 1692, 1770, 1899
MaeII ACGT 1 cut(s) 1482
MaeIII GTNAC 2 cut(s) 1063, 1795
MalI GATC 8 cut(s) 9, 423, 1047, 1398, 1488, 1545, 1608, 1641
MbiI CCGCTC 1 cut(s) 311
MboI GATC 8 cut(s) 7, 421, 1045, 1396, 1486, 1543, 1606, 1639
MboII GAAGA 3 cut(s) 710, 1481, 1751
MfeI CAATTG 2 cut(s) 302, 1879
MflI RGATCY 2 cut(s) 1543, 1639
MhlI GDGCHC 6 cut(s) 69, 150, 254, 465, 619, 931
MlsI TGGCCA 2 cut(s) 1248, 1273
MluNI TGGCCA 2 cut(s) 1248, 1273
Mly113I GGCGCC 1 cut(s) 275
MlyI GAGTC 5 cut(s) 119, 1232, 1304, 1669, 1726
MmeI TCCRAC 4 cut(s) 96, 1183, 1200, 1267
Mox20I TGGCCA 2 cut(s) 1248, 1273
Mph1103I ATGCAT 1 cut(s) 630
MreI CGCCGGCG 1 cut(s) 100
MroNI GCCGGC 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 1434
MscI TGGCCA 2 cut(s) 1248, 1273
MseI TTAA 7 cut(s) 836, 903, 1025, 1175, 1230, 1586, 1620
MslI CAYNNNNRTG 3 cut(s) 456, 521, 1620
Msp20I TGGCCA 2 cut(s) 1248, 1273
MspA1I CMGCKG 1 cut(s) 875
MspI CCGG 5 cut(s) 62, 101, 130, 212, 224
MspR9I CCNGG 7 cut(s) 85, 130, 187, 238, 1031, 1337, 1655
MunI CAATTG 2 cut(s) 302, 1879
Mva1269I GAATGC 3 cut(s) 180, 378, 533
MvaI CCWGG 6 cut(s) 85, 187, 238, 1031, 1337, 1655
MvnI CGCG 1 cut(s) 1858
NaeI GCCGGC 1 cut(s) 102
NarI GGCGCC 1 cut(s) 275
NciI CCSGG 1 cut(s) 130
NdeII GATC 8 cut(s) 7, 421, 1045, 1396, 1486, 1543, 1606, 1639
NgoMIV GCCGGC 1 cut(s) 100
NlaIII CATG 9 cut(s) 154, 455, 494, 505, 520, 628, 1085, 1561, 1832
NlaIV GGNNCC 4 cut(s) 66, 276, 463, 1865
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 2 cut(s) 1063, 1795
NsbI TGCGCA 2 cut(s) 387, 561
NsiI ATGCAT 1 cut(s) 630
NspI RCATGY 4 cut(s) 505, 628, 1085, 1832
PaeI GCATGC 2 cut(s) 1085, 1832
PagI TCATGA 1 cut(s) 516
PasI CCCWGGG 1 cut(s) 84
PceI AGGCCT 1 cut(s) 1566
PciI ACATGT 1 cut(s) 501
PctI GAATGC 3 cut(s) 180, 378, 533
PdiI GCCGGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 1434
PfeI GAWTC 8 cut(s) 95, 869, 952, 1206, 1364, 1372, 1574, 1781
PfoI TCCNGGA 1 cut(s) 1335
PkrI GCNGC 9 cut(s) 60, 163, 217, 247, 450, 481, 824, 877, 1499
PleI GAGTC 5 cut(s) 118, 1231, 1303, 1669, 1726
PluTI GGCGCC 1 cut(s) 278
PpsI GAGTC 5 cut(s) 118, 1231, 1303, 1669, 1726
PpuMI RGGWCCY 1 cut(s) 19
PscI ACATGT 1 cut(s) 501
PshBI ATTAAT 2 cut(s) 836, 1586
Psp124BI GAGCTC 1 cut(s) 150
Psp1406I AACGTT 1 cut(s) 1482
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 6 cut(s) 83, 185, 236, 1029, 1335, 1653
PspFI CCCAGC 1 cut(s) 1049
PspGI CCWGG 6 cut(s) 83, 185, 236, 1029, 1335, 1653
PspN4I GGNNCC 4 cut(s) 66, 276, 463, 1865
PspOMI GGGCCC 1 cut(s) 461
PspPI GGNCC 6 cut(s) 19, 189, 461, 462, 856, 1863
PspPPI RGGWCCY 1 cut(s) 19
PstI CTGCAG 1 cut(s) 880
PsuI RGATCY 2 cut(s) 1543, 1639
PvuII CAGCTG 1 cut(s) 875
RseI CAYNNNNRTG 3 cut(s) 456, 521, 1620
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 7 cut(s) 836, 903, 1025, 1175, 1230, 1586, 1620
SatI GCNGC 9 cut(s) 59, 162, 216, 246, 449, 480, 823, 876, 1498
Sau3AI GATC 8 cut(s) 7, 421, 1045, 1396, 1486, 1543, 1606, 1639
Sau96I GGNCC 6 cut(s) 19, 189, 461, 462, 856, 1863
SchI GAGTC 5 cut(s) 119, 1232, 1304, 1669, 1726
ScrFI CCNGG 7 cut(s) 85, 130, 187, 238, 1031, 1337, 1655
SduI GDGCHC 6 cut(s) 69, 150, 254, 465, 619, 931
SfaNI GCATC 6 cut(s) 319, 637, 680, 691, 1378, 1882
SfcI CTRYAG 1 cut(s) 876
SfoI GGCGCC 1 cut(s) 276
SgrAI CRCCGGYG 1 cut(s) 100
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 3 cut(s) 456, 521, 1620
SmlI CTYRAG 2 cut(s) 851, 1568
SmoI CTYRAG 2 cut(s) 851, 1568
SphI GCATGC 2 cut(s) 1085, 1832
SseBI AGGCCT 1 cut(s) 1566
SsiI CCGC 9 cut(s) 5, 59, 73, 104, 245, 248, 311, 326, 1116
SspDI GGCGCC 1 cut(s) 274
SspMI CTAG 7 cut(s) 443, 605, 657, 941, 1692, 1770, 1899
SstI GAGCTC 1 cut(s) 150
StuI AGGCCT 1 cut(s) 1566
StyD4I CCNGG 7 cut(s) 83, 128, 185, 236, 1029, 1335, 1653
StyI CCWWGG 2 cut(s) 372, 1691
TaaI ACNGT 4 cut(s) 544, 1195, 1218, 1309
TaiI ACGT 1 cut(s) 1485
TaqI TCGA 4 cut(s) 180, 347, 434, 674
TauI GCSGC 2 cut(s) 61, 248
TfiI GAWTC 8 cut(s) 95, 869, 952, 1206, 1364, 1372, 1574, 1781
Tru1I TTAA 7 cut(s) 836, 903, 1025, 1175, 1230, 1586, 1620
Tru9I TTAA 7 cut(s) 836, 903, 1025, 1175, 1230, 1586, 1620
TscAI CASTG 8 cut(s) 39, 395, 954, 1072, 1221, 1336, 1686, 1767
TseFI GTSAC 2 cut(s) 1063, 1795
TseI GCWGC 7 cut(s) 161, 215, 448, 479, 822, 875, 1497
Tsp45I GTSAC 2 cut(s) 1063, 1795
TspDTI ATGAA 8 cut(s) 305, 468, 526, 846, 1362, 1408, 1427, 1794
TspGWI ACGGA 2 cut(s) 220, 615
TspRI CASTG 8 cut(s) 39, 395, 954, 1072, 1221, 1336, 1686, 1767
VneI GTGCAC 2 cut(s) 615, 927
VpaK11BI GGWCC 1 cut(s) 19
VspI ATTAAT 2 cut(s) 836, 1586
XapI RAATTY 2 cut(s) 52, 1803
XceI RCATGY 4 cut(s) 505, 628, 1085, 1832
XmaJI CCTAGG 1 cut(s) 1691
XmnI GAANNNNTTC 1 cut(s) 1434
XspI CTAG 7 cut(s) 443, 605, 657, 941, 1692, 1770, 1899
Zsp2I ATGCAT 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.