MD10G1039400.v1.1

F-actin-capping protein subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010468
Physical Location & Seq
Forward (+)
5178976 .. 5181381
2406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1039400.v1.1.491

Sequence Viewer

Length: 519 bp
ATGGAAGCAGAGATGGGACTGATGCGTCGGATGCCTCCCAAGTACACCGAGACGGCTCTATCCGCCCTTCTCTCCCTTCTCCCCCAGCACTCCTCTGATCTCCTCTCTCAAGTCGATCAGCCCCTCCAGGTTTTGTGCGATATCGAATACGGAAAGCAGTTCATTTTGTGTGAATATAACAGAGACGCAGACTCTTACAGATCGCCTTGGTCAAACAAATATCATCCGCCACTAGAAGACAGCAGCCTCCCATCTTTAGAGTTGAGGAAACTTGAAGTTGAAGCAAACGACATCTTCGCCATTTATCTTGACCAGTACTATGAAGGTGGCATTTCGTCAGTTTATATGTGGGAGGATGATAACGAAGGTTTTGTAGGCTGCTTTTTAATAAAGAGAAATGATGGCTCCAAGACAGGACAAGGTAGGAGAGGTTATCTGCAGGAGGGGGCATGGGATGCTATACATGTGATTGAGGTAAACTCTACTTGTCATGGTAGGAACAACGTGGATCTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F_actin_cap_B

Protein Analysis

173

Amino Acids

19.72

Weight (kDa)

4.64

Isoelectric Point (pI)

57.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F_actin_cap_B PF01115 1 - 165 8.7e-65 F-actin capping protein, beta subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 24
AciI CCGC 2 cut(s) 63, 227
AclWI GGATC 1 cut(s) 516
AfaI GTAC 2 cut(s) 44, 317
AflIII ACRYGT 1 cut(s) 463
AgsI TTSAA 2 cut(s) 275, 281
AjnI CCWGG 1 cut(s) 126
Alw26I GTCTC 2 cut(s) 44, 177
AlwI GGATC 1 cut(s) 516
ApeKI GCWGC 2 cut(s) 243, 378
BbsI GAAGAC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 255, 365
BccI CCATC 3 cut(s) 7, 259, 395
BceAI ACGGC 1 cut(s) 69
BciT130I CCWGG 1 cut(s) 128
BcoDI GTCTC 2 cut(s) 44, 177
BfaI CTAG 1 cut(s) 233
BfmI CTRYAG 1 cut(s) 437
BisI GCNGC 2 cut(s) 244, 379
BlsI GCNGC 2 cut(s) 245, 380
BmcAI AGTACT 1 cut(s) 317
Bme1390I CCNGG 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 3 cut(s) 12, 21, 445
BpiI GAAGAC 1 cut(s) 243
BplI GAGNNNNNCTC 2 cut(s) 464, 496
BpmI CTGGAG 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 313
BseBI CCWGG 1 cut(s) 128
BseDI CCNNGG 1 cut(s) 206
BseGI GGATG 4 cut(s) 36, 223, 361, 460
BseNI ACTGG 1 cut(s) 313
BseRI GAGGAG 2 cut(s) 82, 92
BseXI GCAGC 2 cut(s) 255, 365
BseYI CCCAGC 1 cut(s) 84
BslFI GGGAC 1 cut(s) 30
BsmAI GTCTC 2 cut(s) 44, 177
BsmBI CGTCTC 2 cut(s) 44, 177
BsmFI GGGAC 1 cut(s) 30
Bsp143I GATC 4 cut(s) 97, 115, 200, 508
BspACI CCGC 2 cut(s) 63, 227
BspLI GGNNCC 1 cut(s) 406
BspMAI CTGCAG 1 cut(s) 441
BspPI GGATC 1 cut(s) 516
BsrI ACTGG 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 206
BssMI GATC 4 cut(s) 97, 115, 200, 508
BssT1I CCWWGG 1 cut(s) 206
Bst2UI CCWGG 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 455
BstF5I GGATG 4 cut(s) 36, 223, 361, 460
BstKTI GATC 4 cut(s) 100, 118, 203, 511
BstMAI GTCTC 2 cut(s) 44, 177
BstMBI GATC 4 cut(s) 97, 115, 200, 508
BstMWI GCNNNNNNNGC 3 cut(s) 31, 62, 455
BstNI CCWGG 1 cut(s) 128
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 126
BstSFI CTRYAG 1 cut(s) 437
BstV1I GCAGC 2 cut(s) 255, 365
BstV2I GAAGAC 1 cut(s) 243
BstX2I RGATCY 1 cut(s) 508
BstYI RGATCY 1 cut(s) 508
BtsCI GGATG 4 cut(s) 36, 223, 361, 460
CseI GACGC 2 cut(s) 14, 194
Csp6I GTAC 2 cut(s) 43, 316
CviAII CATG 3 cut(s) 450, 464, 491
CviJI RGCY 5 cut(s) 56, 121, 246, 378, 405
CviKI_1 RGCY 5 cut(s) 56, 121, 246, 378, 405
CviQI GTAC 2 cut(s) 43, 316
DpnI GATC 4 cut(s) 99, 117, 202, 510
DpnII GATC 4 cut(s) 97, 115, 200, 508
DrdI GACNNNNNNGTC 1 cut(s) 24
DseDI GACNNNNNNGTC 1 cut(s) 24
EciI GGCGGA 2 cut(s) 52, 216
Eco130I CCWWGG 1 cut(s) 206
Eco32I GATATC 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 126
EcoRV GATATC 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 206
Esp3I CGTCTC 2 cut(s) 44, 177
FaeI CATG 3 cut(s) 453, 467, 494
FaiI YATR 8 cut(s) 177, 321, 345, 347, 451, 461, 465, 492
FaqI GGGAC 1 cut(s) 30
FatI CATG 3 cut(s) 449, 463, 490
Fnu4HI GCNGC 2 cut(s) 244, 379
FokI GGATG 4 cut(s) 43, 210, 368, 467
Fsp4HI GCNGC 2 cut(s) 244, 379
FspBI CTAG 1 cut(s) 233
GluI GCNGC 2 cut(s) 244, 379
GsaI CCCAGC 1 cut(s) 88
GsuI CTGGAG 1 cut(s) 110
HgaI GACGC 2 cut(s) 14, 194
Hin1II CATG 3 cut(s) 453, 467, 494
HinfI GANTC 1 cut(s) 191
Hpy166II GTNNAC 2 cut(s) 45, 478
Hpy188I TCNGA 2 cut(s) 30, 97
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 2 cut(s) 45, 478
Hpy99I CGWCG 1 cut(s) 30
HpyAV CCTTC 4 cut(s) 77, 86, 317, 359
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 1 cut(s) 439
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 62, 455
HpySE526I ACGT 1 cut(s) 504
Hsp92II CATG 3 cut(s) 453, 467, 494
Kzo9I GATC 4 cut(s) 97, 115, 200, 508
LmnI GCTCC 1 cut(s) 410
LpnPI CCDG 6 cut(s) 98, 113, 140, 326, 399, 425
Lsp1109I GCAGC 2 cut(s) 255, 365
LweI GCATC 3 cut(s) 12, 21, 445
MaeI CTAG 1 cut(s) 233
MaeII ACGT 1 cut(s) 504
MalI GATC 4 cut(s) 99, 117, 202, 510
MboI GATC 4 cut(s) 97, 115, 200, 508
MboII GAAGA 2 cut(s) 248, 286
MflI RGATCY 1 cut(s) 508
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 8
MseI TTAA 1 cut(s) 386
MspR9I CCNGG 1 cut(s) 128
MvaI CCWGG 1 cut(s) 128
MwoI GCNNNNNNNGC 3 cut(s) 31, 62, 455
NdeII GATC 4 cut(s) 97, 115, 200, 508
NlaIII CATG 3 cut(s) 453, 467, 494
NlaIV GGNNCC 1 cut(s) 406
NspI RCATGY 1 cut(s) 467
PciI ACATGT 1 cut(s) 463
PkrI GCNGC 2 cut(s) 245, 380
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PscI ACATGT 1 cut(s) 463
Psp6I CCWGG 1 cut(s) 126
PspFI CCCAGC 1 cut(s) 84
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 406
PstI CTGCAG 1 cut(s) 441
PsuI RGATCY 1 cut(s) 508
RsaI GTAC 2 cut(s) 44, 317
RsaNI GTAC 2 cut(s) 43, 316
SaqAI TTAA 1 cut(s) 386
SatI GCNGC 2 cut(s) 244, 379
Sau3AI GATC 4 cut(s) 97, 115, 200, 508
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 128
SetI ASST 7 cut(s) 132, 328, 370, 424, 433, 477, 507
SfaNI GCATC 3 cut(s) 12, 21, 445
SfcI CTRYAG 1 cut(s) 437
SmlI CTYRAG 1 cut(s) 108
SmoI CTYRAG 1 cut(s) 108
SsiI CCGC 2 cut(s) 63, 227
SspMI CTAG 1 cut(s) 233
StyD4I CCNGG 1 cut(s) 126
StyI CCWWGG 1 cut(s) 206
TaiI ACGT 1 cut(s) 507
TaqI TCGA 2 cut(s) 114, 144
TatI WGTACW 2 cut(s) 42, 315
Tru1I TTAA 1 cut(s) 386
Tru9I TTAA 1 cut(s) 386
TseI GCWGC 2 cut(s) 243, 378
TspDTI ATGAA 2 cut(s) 151, 336
TspGWI ACGGA 1 cut(s) 165
XceI RCATGY 1 cut(s) 467
XspI CTAG 1 cut(s) 233
ZrmI AGTACT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.