MD10G1107900.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013727
Physical Location & Seq
Reverse (-)
17757156 .. 17763773
6618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1107900.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGAGGAGCTGAGTGAAAATTGCTTCCATGAAGCCACCTACCTAGACCAAGAAGGTCAATTAAGAACTTCAAGAGTGCCCGTGGTTCAAGAACTCGCTCGGCGAGGATTTAAACATTTGCCTGAGAGATTCATAAGGGTGCACCCTGAACACGATTTTGACGTTGTCAACTTATCTTTGCTTAATAATGTCTCAGAAGTCCTACCATCCATAAGCATGGCTAAATTACGAGCCCGATCTGAGTCCACAGACTGGACCCGAGAGCTGGGCGAGCTAGCAAGTGGTGCTAATACTTGGGGTATGCTCATAATCAAGGACCATGGTGTGCCAGGGCATGTTTTAGATGGTGTGAAGGATGTGGTCAAGGAGTTCTTTGGGTTGTCATTTGAAGAGAAGAAAGCAAGCGTTGGGTCGTATGTGAGTGTGGATAACATGGGGTACGGCCGGAACTTTGTGAAGTCGGAAGATCAACTGTTGGATTGGATTGACCGTGTGACTGTGAAAGCAGCTTCTGTTGGAGCAACCCAAGGGCTCCATGTTTGGCCTCAAAGGCCTGCCGATTTCAGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

191

Amino Acids

21.42

Weight (kDa)

5.49

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 68 - 183 1.9e-17 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 252
AcoI YGGCCR 1 cut(s) 442
AfaI GTAC 1 cut(s) 440
AfiI CCNNNNNNNGG 2 cut(s) 252, 265
AgsI TTSAA 3 cut(s) 72, 89, 389
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 4 cut(s) 10, 265, 274, 509
AluI AGCT 4 cut(s) 10, 265, 274, 509
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 1 cut(s) 196
Alw44I GTGCAC 1 cut(s) 140
AlwNI CAGNNNCTG 1 cut(s) 512
Ama87I CYCGRG 1 cut(s) 258
AoxI GGCC 3 cut(s) 442, 542, 551
ApaLI GTGCAC 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 506
AspS9I GGNCC 2 cut(s) 255, 316
AsuNHI GCTAGC 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 258
AvaII GGWCC 2 cut(s) 255, 316
BaeGI GKGCMC 2 cut(s) 81, 144
BanII GRGCYC 2 cut(s) 235, 534
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 1 cut(s) 518
BccI CCATC 2 cut(s) 214, 338
BceAI ACGGC 1 cut(s) 457
BciT130I CCWGG 1 cut(s) 330
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 2 cut(s) 44, 275
BglI GCCNNNNNGGC 1 cut(s) 550
BisI GCNGC 1 cut(s) 507
BlsI GCNGC 1 cut(s) 508
Bme1390I CCNGG 1 cut(s) 330
Bme18I GGWCC 2 cut(s) 255, 316
BmeT110I CYCGRG 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 255, 316
BmiI GGNNCC 2 cut(s) 257, 533
BmrFI CCNGG 1 cut(s) 330
BmtI GCTAGC 1 cut(s) 278
BsaJI CCNNGG 4 cut(s) 81, 319, 329, 526
Bsc4I CCNNNNNNNGG 2 cut(s) 252, 265
Bse1I ACTGG 1 cut(s) 257
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 4 cut(s) 81, 319, 329, 526
BseGI GGATG 2 cut(s) 206, 361
BseLI CCNNNNNNNGG 2 cut(s) 252, 265
BseMII CTCAG 3 cut(s) 114, 207, 231
BseNI ACTGG 1 cut(s) 257
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 2 cut(s) 81, 144
BseX3I CGGCCG 1 cut(s) 442
BseXI GCAGC 1 cut(s) 518
BseYI CCCAGC 1 cut(s) 265
Bsh1285I CGRYCG 1 cut(s) 445
BshFI GGCC 3 cut(s) 444, 544, 553
BsiEI CGRYCG 1 cut(s) 445
BsiHKAI GWGCWC 1 cut(s) 144
BsiHKCI CYCGRG 1 cut(s) 258
BsiSI CCGG 1 cut(s) 445
BslI CCNNNNNNNGG 2 cut(s) 252, 265
BsmAI GTCTC 1 cut(s) 196
BsnI GGCC 3 cut(s) 444, 544, 553
BsoBI CYCGRG 1 cut(s) 258
Bsp1286I GDGCHC 4 cut(s) 81, 144, 235, 534
Bsp143I GATC 2 cut(s) 236, 466
Bsp19I CCATGG 1 cut(s) 319
BspANI GGCC 3 cut(s) 444, 544, 553
BspCNI CTCAG 3 cut(s) 115, 206, 232
BspLI GGNNCC 2 cut(s) 257, 533
BspOI GCTAGC 1 cut(s) 278
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 4 cut(s) 81, 319, 329, 526
BssMI GATC 2 cut(s) 236, 466
BssT1I CCWWGG 2 cut(s) 319, 526
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 3 cut(s) 474, 491, 499
Bst6I CTCTTC 1 cut(s) 384
BstAPI GCANNNNNTGC 1 cut(s) 284
BstC8I GCNNGC 4 cut(s) 272, 276, 403, 555
BstDEI CTNAG 4 cut(s) 11, 123, 193, 240
BstDSI CCRYGG 2 cut(s) 81, 319
BstF5I GGATG 2 cut(s) 206, 361
BstKTI GATC 2 cut(s) 239, 469
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 2 cut(s) 236, 466
BstMCI CGRYCG 1 cut(s) 445
BstMWI GCNNNNNNNGC 3 cut(s) 271, 284, 550
BstNI CCWGG 1 cut(s) 330
BstNSI RCATGY 1 cut(s) 338
BstSCI CCNGG 1 cut(s) 328
BstSLI GKGCMC 2 cut(s) 81, 144
BstV1I GCAGC 1 cut(s) 518
BstXI CCANNNNNNTGG 1 cut(s) 217
BstZI CGGCCG 1 cut(s) 442
BsuRI GGCC 3 cut(s) 444, 544, 553
BtgI CCRYGG 2 cut(s) 81, 319
BtsCI GGATG 2 cut(s) 206, 361
Cac8I GCNNGC 4 cut(s) 272, 276, 403, 555
CaiI CAGNNNCTG 1 cut(s) 512
Cfr13I GGNCC 2 cut(s) 255, 316
Csp6I GTAC 1 cut(s) 439
CviAII CATG 6 cut(s) 29, 217, 320, 335, 433, 536
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 4 cut(s) 11, 123, 193, 240
DpnI GATC 2 cut(s) 238, 468
DpnII GATC 2 cut(s) 236, 466
DraI TTTAAA 1 cut(s) 112
EaeI YGGCCR 1 cut(s) 442
EagI CGGCCG 1 cut(s) 442
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
EclXI CGGCCG 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 319, 526
Eco147I AGGCCT 1 cut(s) 553
Eco24I GRGCYC 2 cut(s) 235, 534
Eco47I GGWCC 2 cut(s) 255, 316
Eco52I CGGCCG 1 cut(s) 442
Eco88I CYCGRG 1 cut(s) 258
EcoRII CCWGG 1 cut(s) 328
EcoT14I CCWWGG 2 cut(s) 319, 526
EcoT38I GRGCYC 2 cut(s) 235, 534
ErhI CCWWGG 2 cut(s) 319, 526
FaeI CATG 6 cut(s) 32, 220, 323, 338, 436, 539
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FatI CATG 6 cut(s) 28, 216, 319, 334, 432, 535
Fnu4HI GCNGC 1 cut(s) 507
FokI GGATG 2 cut(s) 193, 368
FriOI GRGCYC 2 cut(s) 235, 534
Fsp4HI GCNGC 1 cut(s) 507
FspBI CTAG 2 cut(s) 44, 275
GluI GCNGC 1 cut(s) 507
GsaI CCCAGC 1 cut(s) 269
HaeIII GGCC 3 cut(s) 444, 544, 553
HapII CCGG 1 cut(s) 445
Hin1II CATG 6 cut(s) 32, 220, 323, 338, 436, 539
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HinfI GANTC 2 cut(s) 129, 242
HpaII CCGG 1 cut(s) 445
Hpy166II GTNNAC 3 cut(s) 142, 169, 246
Hpy188I TCNGA 3 cut(s) 196, 241, 463
Hpy188III TCNNGA 2 cut(s) 72, 89
Hpy8I GTNNAC 3 cut(s) 142, 169, 246
HpyAV CCTTC 2 cut(s) 47, 346
HpyCH4III ACNGT 3 cut(s) 474, 491, 499
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 1 cut(s) 142
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 284, 550
HpyF3I CTNAG 4 cut(s) 11, 123, 193, 240
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 6 cut(s) 32, 220, 323, 338, 436, 539
Kzo9I GATC 2 cut(s) 236, 466
LmnI GCTCC 3 cut(s) 7, 518, 537
LpnPI CCDG 8 cut(s) 135, 159, 238, 251, 315, 342, 458, 567
Lsp1109I GCAGC 1 cut(s) 518
MaeI CTAG 2 cut(s) 44, 275
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 1 cut(s) 493
MalI GATC 2 cut(s) 238, 468
MboI GATC 2 cut(s) 236, 466
MboII GAAGA 3 cut(s) 401, 406, 476
MhlI GDGCHC 4 cut(s) 81, 144, 235, 534
MluCI AATT 3 cut(s) 19, 59, 224
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 3 cut(s) 441, 456, 496
MnlI CCTC 2 cut(s) 98, 555
MseI TTAA 4 cut(s) 62, 111, 183, 571
MslI CAYNNNNRTG 2 cut(s) 137, 215
MspI CCGG 1 cut(s) 445
MspR9I CCNGG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 330
MwoI GCNNNNNNNGC 3 cut(s) 271, 284, 550
NcoI CCATGG 1 cut(s) 319
NdeII GATC 2 cut(s) 236, 466
NheI GCTAGC 1 cut(s) 274
NlaIII CATG 6 cut(s) 32, 220, 323, 338, 436, 539
NlaIV GGNNCC 2 cut(s) 257, 533
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 1 cut(s) 493
NspI RCATGY 1 cut(s) 338
PceI AGGCCT 1 cut(s) 553
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PfeI GAWTC 1 cut(s) 129
PflFI GACNNNGTC 1 cut(s) 164
PflMI CCANNNNNTGG 1 cut(s) 252
PkrI GCNGC 1 cut(s) 508
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 328
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 2 cut(s) 257, 533
PspPI GGNCC 2 cut(s) 255, 316
PstNI CAGNNNCTG 1 cut(s) 512
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
RseI CAYNNNNRTG 2 cut(s) 137, 215
SaqAI TTAA 4 cut(s) 62, 111, 183, 571
SatI GCNGC 1 cut(s) 507
Sau3AI GATC 2 cut(s) 236, 466
Sau96I GGNCC 2 cut(s) 255, 316
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 330
SduI GDGCHC 4 cut(s) 81, 144, 235, 534
SetI ASST 8 cut(s) 12, 41, 45, 58, 165, 267, 276, 511
SfiI GGCCNNNNNGGCC 1 cut(s) 550
SinI GGWCC 2 cut(s) 255, 316
SmiMI CAYNNNNRTG 2 cut(s) 137, 215
Sse9I AATT 3 cut(s) 19, 59, 224
SseBI AGGCCT 1 cut(s) 553
SspMI CTAG 2 cut(s) 44, 275
StuI AGGCCT 1 cut(s) 553
StyD4I CCNGG 1 cut(s) 328
StyI CCWWGG 2 cut(s) 319, 526
TaaI ACNGT 3 cut(s) 474, 491, 499
TaiI ACGT 1 cut(s) 165
TasI AATT 3 cut(s) 19, 59, 224
TfiI GAWTC 1 cut(s) 129
Tru1I TTAA 4 cut(s) 62, 111, 183, 571
Tru9I TTAA 4 cut(s) 62, 111, 183, 571
TseFI GTSAC 1 cut(s) 493
TseI GCWGC 1 cut(s) 506
Tsp45I GTSAC 1 cut(s) 493
TspDTI ATGAA 2 cut(s) 45, 121
Tth111I GACNNNGTC 1 cut(s) 164
Van91I CCANNNNNTGG 1 cut(s) 252
VneI GTGCAC 1 cut(s) 140
VpaK11BI GGWCC 2 cut(s) 255, 316
XceI RCATGY 1 cut(s) 338
XspI CTAG 2 cut(s) 44, 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.