Rroxscaffold_7G00212570

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013727
Physical Location & Seq
Reverse (-)
63074696 .. 63084599
9904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00212570.1

Sequence Viewer

Length: 345 bp
ATGAGGTTTTCGAGAGAAGTCCGCAGCTGGTGGCCGATGAATGGATTCCGGATAGTCTTCAAATTTCAAGTTCAACGACTCGACAAGGACTCAAACACCGATAGGAGAGGTTCGTGGCAGCGCAACCTGAACACGACTTTATCCAGCCATCATGTCTCGGAACCCCTACCATCCATATACATGACCAAATTACGAGGCAACGATCGAGCCGAAGAGCTGGCCAAGCTACTAATTAGAGCTAAGACATGGGGTATGGTTGTAGTTGAAGGGCACGGTGTAGCCGAGTACGTTTTGCATGGTGTGAGGGATATGGTGAAGGAGTTCTTTGGGTACGTTGTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

114

Amino Acids

13.4

Weight (kDa)

10.07

Isoelectric Point (pI)

22.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 56 - 109 2.1e-07 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 48
AciI CCGC 1 cut(s) 22
AcoI YGGCCR 2 cut(s) 32, 219
AcsI RAATTY 1 cut(s) 62
AfaI GTAC 2 cut(s) 287, 332
AfiI CCNNNNNNNGG 1 cut(s) 41
AgsI TTSAA 4 cut(s) 61, 68, 74, 266
AluBI AGCT 4 cut(s) 27, 217, 226, 239
AluI AGCT 4 cut(s) 27, 217, 226, 239
Alw26I GTCTC 1 cut(s) 160
Aor13HI TCCGGA 1 cut(s) 48
AoxI GGCC 2 cut(s) 32, 219
ApeKI GCWGC 2 cut(s) 24, 118
ApoI RAATTY 1 cut(s) 62
Asp700I GAANNNNTTC 2 cut(s) 44, 320
AspLEI GCGC 1 cut(s) 123
AsuHPI GGTGA 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 273
BalI TGGCCA 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 49
BbvI GCAGC 2 cut(s) 36, 130
BccI CCATC 2 cut(s) 156, 178
BcoDI GTCTC 1 cut(s) 160
BisI GCNGC 2 cut(s) 25, 119
BlsI GCNGC 2 cut(s) 26, 120
BmiI GGNNCC 1 cut(s) 162
BpiI GAAGAC 1 cut(s) 49
BsaWI WCCGGW 1 cut(s) 48
Bsc4I CCNNNNNNNGG 1 cut(s) 41
BseAI TCCGGA 1 cut(s) 48
BseGI GGATG 1 cut(s) 170
BseLI CCNNNNNNNGG 1 cut(s) 41
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 2 cut(s) 36, 130
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 2 cut(s) 34, 221
BsiEI CGRYCG 1 cut(s) 205
BsiSI CCGG 1 cut(s) 49
BslI CCNNNNNNNGG 1 cut(s) 41
BsmAI GTCTC 1 cut(s) 160
BsnI GGCC 2 cut(s) 34, 221
Bsp1286I GDGCHC 1 cut(s) 273
Bsp13I TCCGGA 1 cut(s) 48
Bsp143I GATC 1 cut(s) 202
BspACI CCGC 1 cut(s) 22
BspANI GGCC 2 cut(s) 34, 221
BspEI TCCGGA 1 cut(s) 48
BspLI GGNNCC 1 cut(s) 162
BspQI GCTCTTC 1 cut(s) 207
BssMI GATC 1 cut(s) 202
Bst4CI ACNGT 1 cut(s) 275
Bst6I CTCTTC 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 240
BstF5I GGATG 1 cut(s) 170
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 1 cut(s) 202
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 1 cut(s) 223
BstSLI GKGCMC 1 cut(s) 273
BstV1I GCAGC 2 cut(s) 36, 130
BstV2I GAAGAC 1 cut(s) 49
BsuRI GGCC 2 cut(s) 34, 221
BtsCI GGATG 1 cut(s) 170
Cac8I GCNNGC 1 cut(s) 219
CfoI GCGC 1 cut(s) 123
Csp6I GTAC 2 cut(s) 286, 331
CviAII CATG 4 cut(s) 152, 181, 246, 296
CviJI RGCY 9 cut(s) 27, 34, 147, 209, 217, 221, 226, 239, 281
CviKI_1 RGCY 9 cut(s) 27, 34, 147, 209, 217, 221, 226, 239, 281
CviQI GTAC 2 cut(s) 286, 331
DdeI CTNAG 1 cut(s) 240
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
EaeI YGGCCR 2 cut(s) 32, 219
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
FaeI CATG 4 cut(s) 155, 184, 249, 299
FaiI YATR 8 cut(s) 153, 176, 178, 182, 247, 254, 297, 311
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FatI CATG 4 cut(s) 151, 180, 245, 295
Fnu4HI GCNGC 2 cut(s) 25, 119
FokI GGATG 1 cut(s) 157
Fsp4HI GCNGC 2 cut(s) 25, 119
GlaI GCGC 1 cut(s) 122
GluI GCNGC 2 cut(s) 25, 119
HaeIII GGCC 2 cut(s) 34, 221
HapII CCGG 1 cut(s) 49
HhaI GCGC 1 cut(s) 123
Hin1II CATG 4 cut(s) 155, 184, 249, 299
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HinfI GANTC 3 cut(s) 45, 78, 89
HpaII CCGG 1 cut(s) 49
HphI GGTGA 1 cut(s) 325
Hpy188I TCNGA 1 cut(s) 160
Hpy188III TCNNGA 2 cut(s) 12, 49
HpyAV CCTTC 2 cut(s) 260, 310
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4IV ACGT 2 cut(s) 288, 333
HpyCH4V TGCA 1 cut(s) 295
HpyF10VI GCNNNNNNNGC 1 cut(s) 223
HpyF3I CTNAG 1 cut(s) 240
HpySE526I ACGT 2 cut(s) 288, 333
Hsp92II CATG 4 cut(s) 155, 184, 249, 299
HspAI GCGC 1 cut(s) 121
Kpn2I TCCGGA 1 cut(s) 48
Kzo9I GATC 1 cut(s) 202
LguI GCTCTTC 1 cut(s) 207
LpnPI CCDG 5 cut(s) 13, 62, 140, 157, 203
Lsp1109I GCAGC 2 cut(s) 36, 130
MaeII ACGT 2 cut(s) 288, 333
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 2 cut(s) 49, 224
MhlI GDGCHC 1 cut(s) 273
MlsI TGGCCA 1 cut(s) 221
MluCI AATT 3 cut(s) 62, 188, 231
MluNI TGGCCA 1 cut(s) 221
MlyI GAGTC 2 cut(s) 72, 83
MnlI CCTC 3 cut(s) 101, 188, 297
Mox20I TGGCCA 1 cut(s) 221
MroI TCCGGA 1 cut(s) 48
MroXI GAANNNNTTC 2 cut(s) 44, 320
MscI TGGCCA 1 cut(s) 221
MslI CAYNNNNRTG 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 27
MspI CCGG 1 cut(s) 49
MwoI GCNNNNNNNGC 1 cut(s) 223
NdeII GATC 1 cut(s) 202
NlaIII CATG 4 cut(s) 155, 184, 249, 299
NlaIV GGNNCC 1 cut(s) 162
NmeAIII GCCGAG 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 207
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 279
PdmI GAANNNNTTC 2 cut(s) 44, 320
PfeI GAWTC 1 cut(s) 45
PkrI GCNGC 2 cut(s) 26, 120
Ple19I CGATCG 1 cut(s) 205
PleI GAGTC 2 cut(s) 72, 83
PpsI GAGTC 2 cut(s) 72, 83
PspN4I GGNNCC 1 cut(s) 162
PvuI CGATCG 1 cut(s) 205
PvuII CAGCTG 1 cut(s) 27
RsaI GTAC 2 cut(s) 287, 332
RsaNI GTAC 2 cut(s) 286, 331
RseI CAYNNNNRTG 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 207
SatI GCNGC 2 cut(s) 25, 119
Sau3AI GATC 1 cut(s) 202
SchI GAGTC 2 cut(s) 72, 83
SduI GDGCHC 1 cut(s) 273
SetI ASST 9 cut(s) 8, 29, 112, 129, 219, 228, 241, 291, 336
SmiMI CAYNNNNRTG 1 cut(s) 179
Sse9I AATT 3 cut(s) 62, 188, 231
SsiI CCGC 1 cut(s) 22
TaaI ACNGT 1 cut(s) 275
TaiI ACGT 2 cut(s) 291, 336
TaqI TCGA 3 cut(s) 11, 81, 205
TasI AATT 3 cut(s) 62, 188, 231
TfiI GAWTC 1 cut(s) 45
TseI GCWGC 2 cut(s) 24, 118
TspDTI ATGAA 1 cut(s) 53
XapI RAATTY 1 cut(s) 62
XmnI GAANNNNTTC 2 cut(s) 44, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.