Rw6G004810

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0013727
Physical Location & Seq
Forward (+)
7898669 .. 7899235
567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G004810.1

Sequence Viewer

Length: 567 bp
ATGGAGACCAGTACTGAAAATAATTACTTTCATGAAGCCAGCTACATAGACCAAGCAGGTCAATTACGAACCACAAGAGTGCCTGCAGTTCAAGAACTAGCTCGCCAAGGACTCAAACACCTGCCGGAGAGGTTCGTGGCGCTGCAACCTGAACACGACCAGAACCCGGCTTTATCCAGCTATCATGTCTCAGAACCCCTACCGTCCATAAACATGACCAAATTACGAGGCAACGATCGAGCCGAAGAGCTGGCCAAGCTACTAACTGGAGCTAAGACATGGGGTATGGTTGTAGTTGAAGGCCACGGTGTAGCCGAGTCTGTTTTGCATGGTGTGAGGGATGTGGTGAAGGCGTTCTTTGGGTTGTCGTTTGACGAGAAAAAGGCAAGCGTTGGGTCTTATGTGAGCGTGGATAACATGGGGTATGGCCGGAACTTTGTCAAGTCTGAAGACCAGCCGTTGGATTGGATTGATCGTGTGACTGTGAAAGCAGCTCCGGCCGGAGCAACCGAAGGGCTTCATGTTTGGCCTCAAAGACCTGCTAATTTCAGGTACGTACGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

188

Amino Acids

20.95

Weight (kDa)

6.38

Isoelectric Point (pI)

30.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 67 - 178 1e-18 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 129
Acc36I ACCTGC 3 cut(s) 47, 129, 547
AccB7I CCANNNNNTGG 1 cut(s) 460
AcoI YGGCCR 3 cut(s) 252, 427, 498
AcuI CTGAAG 1 cut(s) 468
AfaI GTAC 3 cut(s) 13, 554, 558
AfiI CCNNNNNNNGG 2 cut(s) 166, 460
AgsI TTSAA 2 cut(s) 92, 299
AleI CACNNNNGTG 1 cut(s) 77
AluBI AGCT 7 cut(s) 42, 101, 180, 250, 259, 272, 494
AluI AGCT 7 cut(s) 42, 101, 180, 250, 259, 272, 494
Alw26I GTCTC 1 cut(s) 193
AoxI GGCC 5 cut(s) 252, 301, 427, 498, 527
ApeKI GCWGC 2 cut(s) 142, 491
Asp700I GAANNNNTTC 2 cut(s) 353, 516
AspLEI GCGC 1 cut(s) 142
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 358
BaeI ACNNNNGTAYC 1 cut(s) 544
BalI TGGCCA 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 456
BbvI GCAGC 2 cut(s) 129, 503
BceAI ACGGC 1 cut(s) 442
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 84
BfoI RGCGCY 1 cut(s) 143
BfuAI ACCTGC 3 cut(s) 47, 129, 547
BisI GCNGC 2 cut(s) 143, 492
BlsI GCNGC 2 cut(s) 144, 493
BmcAI AGTACT 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 167
BmrFI CCNGG 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 456
BpmI CTGGAG 1 cut(s) 288
BpuMI CCSGG 1 cut(s) 167
BsaAI YACGTR 1 cut(s) 556
BsaJI CCNNGG 2 cut(s) 106, 304
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 460
Bse1I ACTGG 2 cut(s) 9, 271
BseDI CCNNGG 2 cut(s) 106, 304
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 2 cut(s) 166, 460
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 2 cut(s) 9, 271
BseX3I CGGCCG 1 cut(s) 498
BseXI GCAGC 2 cut(s) 129, 503
Bsh1285I CGRYCG 2 cut(s) 238, 501
BshFI GGCC 5 cut(s) 254, 303, 429, 500, 529
BsiEI CGRYCG 2 cut(s) 238, 501
BsiSI CCGG 5 cut(s) 125, 167, 430, 497, 501
BsiWI CGTACG 1 cut(s) 556
BslI CCNNNNNNNGG 2 cut(s) 166, 460
BsmAI GTCTC 1 cut(s) 193
BsnI GGCC 5 cut(s) 254, 303, 429, 500, 529
Bsp143I GATC 2 cut(s) 235, 472
BspANI GGCC 5 cut(s) 254, 303, 429, 500, 529
BspCNI CTCAG 1 cut(s) 203
BspHI TCATGA 1 cut(s) 31
BspMAI CTGCAG 1 cut(s) 88
BspMI ACCTGC 3 cut(s) 47, 129, 547
BspQI GCTCTTC 1 cut(s) 240
BsrI ACTGG 2 cut(s) 9, 271
BssECI CCNNGG 2 cut(s) 106, 304
BssMI GATC 2 cut(s) 235, 472
BssT1I CCWWGG 1 cut(s) 106
Bst4CI ACNGT 3 cut(s) 204, 308, 484
Bst6I CTCTTC 1 cut(s) 240
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 5 cut(s) 40, 84, 103, 252, 388
BstDEI CTNAG 2 cut(s) 190, 273
BstDSI CCRYGG 1 cut(s) 304
BstF5I GGATG 1 cut(s) 346
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 2 cut(s) 238, 475
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 2 cut(s) 235, 472
BstMCI CGRYCG 2 cut(s) 238, 501
BstMWI GCNNNNNNNGC 2 cut(s) 256, 497
BstSCI CCNGG 1 cut(s) 165
BstSFI CTRYAG 1 cut(s) 84
BstSNI TACGTA 1 cut(s) 556
BstV1I GCAGC 2 cut(s) 129, 503
BstV2I GAAGAC 1 cut(s) 456
BstZI CGGCCG 1 cut(s) 498
BsuRI GGCC 5 cut(s) 254, 303, 429, 500, 529
BtgI CCRYGG 1 cut(s) 304
BtsCI GGATG 1 cut(s) 346
BveI ACCTGC 3 cut(s) 47, 129, 547
Cac8I GCNNGC 5 cut(s) 40, 84, 103, 252, 388
CciI TCATGA 1 cut(s) 31
CfoI GCGC 1 cut(s) 142
Csp6I GTAC 3 cut(s) 12, 553, 557
CviAII CATG 7 cut(s) 32, 185, 214, 279, 329, 418, 521
CviQI GTAC 3 cut(s) 12, 553, 557
DdeI CTNAG 2 cut(s) 190, 273
DpnI GATC 2 cut(s) 237, 474
DpnII GATC 2 cut(s) 235, 472
EaeI YGGCCR 3 cut(s) 252, 427, 498
EagI CGGCCG 1 cut(s) 498
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
EclXI CGGCCG 1 cut(s) 498
Eco105I TACGTA 1 cut(s) 556
Eco130I CCWWGG 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 498
Eco57I CTGAAG 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 7 cut(s) 35, 188, 217, 282, 332, 421, 524
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FatI CATG 7 cut(s) 31, 184, 213, 278, 328, 417, 520
Fnu4HI GCNGC 2 cut(s) 143, 492
FokI GGATG 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 143, 492
FspBI CTAG 1 cut(s) 98
GlaI GCGC 1 cut(s) 141
GluI GCNGC 2 cut(s) 143, 492
GsuI CTGGAG 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 5 cut(s) 254, 303, 429, 500, 529
HapII CCGG 5 cut(s) 125, 167, 430, 497, 501
HhaI GCGC 1 cut(s) 142
Hin1II CATG 7 cut(s) 35, 188, 217, 282, 332, 421, 524
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HinfI GANTC 2 cut(s) 111, 317
HpaII CCGG 5 cut(s) 125, 167, 430, 497, 501
HphI GGTGA 1 cut(s) 358
Hpy188I TCNGA 2 cut(s) 193, 448
Hpy188III TCNNGA 2 cut(s) 32, 92
HpyAV CCTTC 3 cut(s) 293, 343, 506
HpyCH4III ACNGT 3 cut(s) 204, 308, 484
HpyCH4IV ACGT 2 cut(s) 555, 559
HpyCH4V TGCA 3 cut(s) 86, 145, 328
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 497
HpyF3I CTNAG 2 cut(s) 190, 273
HpySE526I ACGT 2 cut(s) 555, 559
Hsp92II CATG 7 cut(s) 35, 188, 217, 282, 332, 421, 524
HspAI GCGC 1 cut(s) 140
Kzo9I GATC 2 cut(s) 235, 472
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 3 cut(s) 269, 499, 503
Lsp1109I GCAGC 2 cut(s) 129, 503
MaeI CTAG 1 cut(s) 98
MaeII ACGT 2 cut(s) 555, 559
MaeIII GTNAC 1 cut(s) 478
MalI GATC 2 cut(s) 237, 474
MboI GATC 2 cut(s) 235, 472
MboII GAAGA 2 cut(s) 257, 461
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 4 cut(s) 22, 62, 221, 544
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 2 cut(s) 105, 326
MmeI TCCRAC 1 cut(s) 441
MnlI CCTC 4 cut(s) 123, 221, 330, 540
Mox20I TGGCCA 1 cut(s) 254
MroXI GAANNNNTTC 2 cut(s) 353, 516
MscI TGGCCA 1 cut(s) 254
MslI CAYNNNNRTG 2 cut(s) 77, 212
Msp20I TGGCCA 1 cut(s) 254
MspI CCGG 5 cut(s) 125, 167, 430, 497, 501
MspR9I CCNGG 1 cut(s) 167
MwoI GCNNNNNNNGC 2 cut(s) 256, 497
NciI CCSGG 1 cut(s) 167
NdeII GATC 2 cut(s) 235, 472
NlaIII CATG 7 cut(s) 35, 188, 217, 282, 332, 421, 524
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 1 cut(s) 478
OliI CACNNNNGTG 1 cut(s) 77
PagI TCATGA 1 cut(s) 31
PaqCI CACCTGC 1 cut(s) 129
PciSI GCTCTTC 1 cut(s) 240
PcsI WCGNNNNNNNCGW 2 cut(s) 240, 312
PdmI GAANNNNTTC 2 cut(s) 353, 516
Pfl23II CGTACG 1 cut(s) 556
PflMI CCANNNNNTGG 1 cut(s) 460
PkrI GCNGC 2 cut(s) 144, 493
Ple19I CGATCG 1 cut(s) 238
PleI GAGTC 2 cut(s) 105, 325
PpsI GAGTC 2 cut(s) 105, 325
Ppu21I YACGTR 1 cut(s) 556
PspLI CGTACG 1 cut(s) 556
PstI CTGCAG 1 cut(s) 88
PvuI CGATCG 1 cut(s) 238
RsaI GTAC 3 cut(s) 13, 554, 558
RsaNI GTAC 3 cut(s) 12, 553, 557
RseI CAYNNNNRTG 2 cut(s) 77, 212
SapI GCTCTTC 1 cut(s) 240
SatI GCNGC 2 cut(s) 143, 492
Sau3AI GATC 2 cut(s) 235, 472
ScaI AGTACT 1 cut(s) 13
SchI GAGTC 2 cut(s) 105, 326
ScrFI CCNGG 1 cut(s) 167
SfcI CTRYAG 1 cut(s) 84
SmiMI CAYNNNNRTG 2 cut(s) 77, 212
SnaBI TACGTA 1 cut(s) 556
Sse9I AATT 4 cut(s) 22, 62, 221, 544
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 165
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 3 cut(s) 204, 308, 484
TaiI ACGT 2 cut(s) 558, 562
TaqI TCGA 1 cut(s) 238
TasI AATT 4 cut(s) 22, 62, 221, 544
TatI WGTACW 1 cut(s) 11
TseFI GTSAC 1 cut(s) 478
TseI GCWGC 2 cut(s) 142, 491
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 3 cut(s) 20, 48, 509
Van91I CCANNNNNTGG 1 cut(s) 460
XmnI GAANNNNTTC 2 cut(s) 353, 516
XspI CTAG 1 cut(s) 98
ZrmI AGTACT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.