MD10G1116600.v1.1

5'-methylthioadenosine S-adenosylhomocysteine nucleosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010266
Physical Location & Seq
Forward (+)
19427237 .. 19431320
4084 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1116600.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGCTCCTCACGGTGACAAATCGGACGCTGCAGTGGAAGCCTTGGTTTCCGAAGCTGAGAAGCGCCCCATTTCCACCATCCTCCTCGTCATCGCTATGCAGACTGAAGCGCTTCCAGTGGTGAACAAGTTCAATCTCACAGAGGATCCTGACTCCGTGTTCCCAAACGGGGTGCCTTGGGTCCGGTATCACGGTTCATACAAAGATCTGAAGATTAATCTGGTTTGGCCTGGAAAAGATTCTTCGTTGGGGGTTGATTGTGTAGGCACGGTTTCTGCGTCTCTTGTGACCTATGCTTCCATTCAAGCATTACAACCAGACCTAATTATCAATGCAGGCACTGCTGGTGGTTTTAAGGCCAAAGGAGCTTGCATCGGTGATTTGTATCTCGCATGTGATGTTGCTTACCATGATAGAAGGATACCTATTCCTGTCTTTGATCTATATGGAGTTGGTTTGCGGCAAGCCTGCGCATCACCGAATCTTCAAAAGGAACTTAACCTGAAGGTGGGTAAACTGTCTACCGGAAACTCTCTAGATATGTCCCCACAGGACGAAGCATCGATCATGGCAAATGATGCTACAATTAAAGACATGGAGGGTGCAGCTGTTGCTTACGTTGCAGATATGTTGAAAGTCCCAATCATATTTGTTAAAGCTGTGACTGATATAGTAGACGGTGAAAAACCAACCGCGGAAGAATTCTTGCAAAATTTGGCAGCTGTAACTGCTGCTCTTGAAGAGAAGGTCACCCAAGTCATAGACTTTATTAATGGAAAGTGCCTCTCTGATCTTTGA

Protein Analysis

266

Amino Acids

28.25

Weight (kDa)

4.66

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNP_UDP_1 PF01048 26 - 254 2.4e-28 Phosphorylase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34840 AT4G34840 AT4G34840 AT4G34840 AT4G34840 AT4G38800
fragaria_vesca FvH4_2g02150
malus_domestica MD10G1116600.v1.1
prunus_persica Prupe.8G159400_v2.0.a1 Prupe.8G159500_v2.0.a1
pyrus_communis pycom10g10140
rosa_chinensis RchiOBHm_Chr6g0245651
rosa_laevigata RLG00000015296
rosa_multiflora Rmu_sc0016035.1_g000004
rosa_roxburghii Rroxscaffold_7G00215150
rosa_rugosa Rorug05G0515300
rosa_samantha Rh6AG030000 Rh6BG027400 Rh6CG026700 Rh6DG026100
rosa_wichuraiana Rw6G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 472
AccB1I GGYRCC 1 cut(s) 172
AccI GTMKAC 2 cut(s) 521, 675
AccII CGCG 1 cut(s) 695
AciI CCGC 3 cut(s) 460, 693, 695
AclWI GGATC 2 cut(s) 140, 153
AcsI RAATTY 2 cut(s) 701, 712
AcuI CTGAAG 3 cut(s) 126, 230, 524
AfeI AGCGCT 1 cut(s) 111
AfiI CCNNNNNNNGG 2 cut(s) 169, 508
AgsI TTSAA 5 cut(s) 133, 305, 488, 634, 740
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 5 cut(s) 56, 368, 608, 659, 722
AluI AGCT 5 cut(s) 56, 368, 608, 659, 722
Alw26I GTCTC 1 cut(s) 285
AlwI GGATC 2 cut(s) 140, 153
AlwNI CAGNNNCTG 1 cut(s) 341
Aor51HI AGCGCT 1 cut(s) 111
AoxI GGCC 2 cut(s) 227, 357
ApeKI GCWGC 4 cut(s) 29, 605, 719, 731
ApoI RAATTY 2 cut(s) 701, 712
AseI ATTAAT 2 cut(s) 216, 771
Asp700I GAANNNNTTC 3 cut(s) 111, 128, 238
AspLEI GCGC 3 cut(s) 66, 112, 473
AspS9I GGNCC 1 cut(s) 181
AsuHPI GGTGA 6 cut(s) 26, 133, 389, 468, 692, 742
AvaII GGWCC 1 cut(s) 181
BamHI GGATCC 1 cut(s) 145
BanI GGYRCC 1 cut(s) 172
BbvI GCAGC 4 cut(s) 16, 617, 718, 731
BccI CCATC 1 cut(s) 86
BciT130I CCWGG 1 cut(s) 231
BciVI GTATCC 1 cut(s) 414
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 2 cut(s) 67, 113
BfuI GTATCC 1 cut(s) 414
BglII AGATCT 1 cut(s) 205
BisI GCNGC 5 cut(s) 30, 461, 606, 720, 732
BlsI GCNGC 5 cut(s) 31, 462, 607, 721, 733
Bme1390I CCNGG 1 cut(s) 231
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 4 cut(s) 6, 147, 174, 182
BmrFI CCNGG 1 cut(s) 231
BmsI GCATC 4 cut(s) 381, 482, 568, 569
Bsa29I ATCGAT 1 cut(s) 563
BsaBI GATNNNNATC 1 cut(s) 384
BsaJI CCNNGG 3 cut(s) 42, 176, 693
BsaWI WCCGGW 2 cut(s) 183, 524
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 508
Bse1I ACTGG 1 cut(s) 116
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 1 cut(s) 231
BseCI ATCGAT 1 cut(s) 563
BseDI CCNNGG 3 cut(s) 42, 176, 693
BseGI GGATG 1 cut(s) 78
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 2 cut(s) 169, 508
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 116
BseRI GAGGAG 1 cut(s) 74
BseXI GCAGC 4 cut(s) 16, 617, 718, 731
BsgI GTGCAG 1 cut(s) 624
Bsh1236I CGCG 1 cut(s) 695
BshFI GGCC 2 cut(s) 229, 359
BshNI GGYRCC 1 cut(s) 172
BshVI ATCGAT 1 cut(s) 563
BsiSI CCGG 2 cut(s) 184, 525
BslFI GGGAC 2 cut(s) 529, 623
BslI CCNNNNNNNGG 2 cut(s) 169, 508
BsmAI GTCTC 1 cut(s) 285
BsmBI CGTCTC 1 cut(s) 285
BsmFI GGGAC 2 cut(s) 529, 623
BsnI GGCC 2 cut(s) 229, 359
Bsp143I GATC 5 cut(s) 145, 205, 439, 564, 790
BspACI CCGC 3 cut(s) 460, 693, 695
BspANI GGCC 2 cut(s) 229, 359
BspCNI CTCAG 1 cut(s) 49
BspDI ATCGAT 1 cut(s) 563
BspFNI CGCG 1 cut(s) 695
BspLI GGNNCC 4 cut(s) 6, 147, 174, 182
BspMAI CTGCAG 1 cut(s) 34
BspPI GGATC 2 cut(s) 140, 153
BspT107I GGYRCC 1 cut(s) 172
BsrI ACTGG 1 cut(s) 116
BssECI CCNNGG 3 cut(s) 42, 176, 693
BssMI GATC 5 cut(s) 145, 205, 439, 564, 790
BssT1I CCWWGG 2 cut(s) 42, 176
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 5 cut(s) 14, 194, 271, 519, 680
Bst6I CTCTTC 1 cut(s) 735
BstAPI GCANNNNNTGC 3 cut(s) 341, 578, 611
BstC8I GCNNGC 4 cut(s) 337, 370, 465, 469
BstDEI CTNAG 1 cut(s) 57
BstDSI CCRYGG 1 cut(s) 693
BstEII GGTNACC 1 cut(s) 748
BstF5I GGATG 1 cut(s) 78
BstFNI CGCG 1 cut(s) 695
BstH2I RGCGCY 2 cut(s) 67, 113
BstHHI GCGC 3 cut(s) 66, 112, 473
BstKTI GATC 5 cut(s) 148, 208, 442, 567, 793
BstMAI GTCTC 1 cut(s) 285
BstMBI GATC 5 cut(s) 145, 205, 439, 564, 790
BstMWI GCNNNNNNNGC 7 cut(s) 38, 341, 365, 578, 611, 620, 728
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 396
BstPI GGTNACC 1 cut(s) 748
BstSCI CCNGG 1 cut(s) 229
BstSFI CTRYAG 1 cut(s) 30
BstUI CGCG 1 cut(s) 695
BstV1I GCAGC 4 cut(s) 16, 617, 718, 731
BstX2I RGATCY 2 cut(s) 145, 205
BstYI RGATCY 2 cut(s) 145, 205
Bsu15I ATCGAT 1 cut(s) 563
BsuI GTATCC 1 cut(s) 414
BsuRI GGCC 2 cut(s) 229, 359
BsuTUI ATCGAT 1 cut(s) 563
BtgI CCRYGG 1 cut(s) 693
BtgZI GCGATG 1 cut(s) 76
BtsCI GGATG 1 cut(s) 78
BtsI GCAGTG 2 cut(s) 39, 339
BtsIMutI CAGTG 3 cut(s) 39, 123, 339
Cac8I GCNNGC 4 cut(s) 337, 370, 465, 469
CaiI CAGNNNCTG 1 cut(s) 341
CfoI GCGC 3 cut(s) 66, 112, 473
Cfr13I GGNCC 1 cut(s) 181
Cfr42I CCGCGG 1 cut(s) 696
ClaI ATCGAT 1 cut(s) 563
CseI GACGC 2 cut(s) 35, 267
CviAII CATG 4 cut(s) 393, 410, 568, 595
DdeI CTNAG 1 cut(s) 57
DpnI GATC 5 cut(s) 147, 207, 441, 566, 792
DpnII GATC 5 cut(s) 145, 205, 439, 564, 790
Eam1104I CTCTTC 1 cut(s) 735
EarI CTCTTC 1 cut(s) 735
Eco130I CCWWGG 2 cut(s) 42, 176
Eco47I GGWCC 1 cut(s) 181
Eco47III AGCGCT 1 cut(s) 111
Eco57I CTGAAG 3 cut(s) 126, 230, 524
Eco91I GGTNACC 1 cut(s) 748
EcoO65I GGTNACC 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 701
EcoRII CCWGG 1 cut(s) 229
EcoT14I CCWWGG 2 cut(s) 42, 176
ErhI CCWWGG 2 cut(s) 42, 176
Esp3I CGTCTC 1 cut(s) 285
FaeI CATG 4 cut(s) 396, 413, 571, 598
FaqI GGGAC 2 cut(s) 529, 623
FatI CATG 4 cut(s) 392, 409, 567, 594
FblI GTMKAC 2 cut(s) 521, 675
Fnu4HI GCNGC 5 cut(s) 30, 461, 606, 720, 732
FokI GGATG 1 cut(s) 65
Fsp4HI GCNGC 5 cut(s) 30, 461, 606, 720, 732
FspBI CTAG 1 cut(s) 536
FspI TGCGCA 1 cut(s) 472
GlaI GCGC 3 cut(s) 65, 111, 472
GluI GCNGC 5 cut(s) 30, 461, 606, 720, 732
HaeII RGCGCY 2 cut(s) 67, 113
HaeIII GGCC 2 cut(s) 229, 359
HapII CCGG 2 cut(s) 184, 525
HgaI GACGC 2 cut(s) 35, 267
HhaI GCGC 3 cut(s) 66, 112, 473
Hin1II CATG 4 cut(s) 396, 413, 571, 598
Hin6I GCGC 3 cut(s) 64, 110, 471
HinP1I GCGC 3 cut(s) 64, 110, 471
HinfI GANTC 3 cut(s) 152, 239, 481
HpaII CCGG 2 cut(s) 184, 525
HphI GGTGA 6 cut(s) 26, 133, 389, 468, 692, 742
Hpy166II GTNNAC 4 cut(s) 124, 515, 522, 676
Hpy188I TCNGA 4 cut(s) 25, 52, 210, 790
Hpy188III TCNNGA 3 cut(s) 149, 536, 737
Hpy8I GTNNAC 4 cut(s) 124, 515, 522, 676
HpyAV CCTTC 3 cut(s) 411, 499, 739
HpyCH4III ACNGT 5 cut(s) 14, 194, 271, 519, 680
HpyCH4IV ACGT 1 cut(s) 618
HpyCH4V TGCA 7 cut(s) 32, 100, 335, 372, 605, 623, 709
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 341, 365, 578, 611, 620, 728
HpyF3I CTNAG 1 cut(s) 57
HpySE526I ACGT 1 cut(s) 618
Hsp92II CATG 4 cut(s) 396, 413, 571, 598
HspAI GCGC 3 cut(s) 64, 110, 471
KspI CCGCGG 1 cut(s) 696
Kzo9I GATC 5 cut(s) 145, 205, 439, 564, 790
LmnI GCTCC 2 cut(s) 10, 365
Lsp1109I GCAGC 4 cut(s) 16, 617, 718, 731
LweI GCATC 4 cut(s) 381, 482, 568, 569
MaeI CTAG 1 cut(s) 536
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 5 cut(s) 14, 286, 661, 724, 748
MalI GATC 5 cut(s) 147, 207, 441, 566, 792
MboI GATC 5 cut(s) 145, 205, 439, 564, 790
MboII GAAGA 5 cut(s) 223, 234, 476, 710, 752
MflI RGATCY 2 cut(s) 145, 205
MluCI AATT 4 cut(s) 324, 585, 701, 712
MlyI GAGTC 1 cut(s) 146
MnlI CCTC 6 cut(s) 18, 92, 95, 136, 592, 794
MroXI GAANNNNTTC 3 cut(s) 111, 128, 238
MseI TTAA 6 cut(s) 216, 354, 498, 588, 654, 771
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 3 cut(s) 608, 695, 722
MspI CCGG 2 cut(s) 184, 525
MspR9I CCNGG 1 cut(s) 231
MvaI CCWGG 1 cut(s) 231
MvnI CGCG 1 cut(s) 695
MwoI GCNNNNNNNGC 7 cut(s) 38, 341, 365, 578, 611, 620, 728
NdeII GATC 5 cut(s) 145, 205, 439, 564, 790
NlaIII CATG 4 cut(s) 396, 413, 571, 598
NlaIV GGNNCC 4 cut(s) 6, 147, 174, 182
NmuCI GTSAC 4 cut(s) 14, 286, 661, 748
NsbI TGCGCA 1 cut(s) 472
NspI RCATGY 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 275
PdmI GAANNNNTTC 3 cut(s) 111, 128, 238
PfeI GAWTC 2 cut(s) 239, 481
PkrI GCNGC 5 cut(s) 31, 462, 607, 721, 733
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PshBI ATTAAT 2 cut(s) 216, 771
Psp6I CCWGG 1 cut(s) 229
PspEI GGTNACC 1 cut(s) 748
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 4 cut(s) 6, 147, 174, 182
PspPI GGNCC 1 cut(s) 181
PstI CTGCAG 1 cut(s) 34
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 2 cut(s) 145, 205
PvuII CAGCTG 2 cut(s) 608, 722
RseI CAYNNNNRTG 1 cut(s) 95
SacII CCGCGG 1 cut(s) 696
SaqAI TTAA 6 cut(s) 216, 354, 498, 588, 654, 771
SatI GCNGC 5 cut(s) 30, 461, 606, 720, 732
Sau3AI GATC 5 cut(s) 145, 205, 439, 564, 790
Sau96I GGNCC 1 cut(s) 181
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 231
SfaNI GCATC 4 cut(s) 381, 482, 568, 569
SfcI CTRYAG 1 cut(s) 30
Sfr303I CCGCGG 1 cut(s) 696
SgrBI CCGCGG 1 cut(s) 696
SinI GGWCC 1 cut(s) 181
SmiMI CAYNNNNRTG 1 cut(s) 95
Sse9I AATT 4 cut(s) 324, 585, 701, 712
SsiI CCGC 3 cut(s) 460, 693, 695
SspMI CTAG 1 cut(s) 536
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 2 cut(s) 42, 176
TaaI ACNGT 5 cut(s) 14, 194, 271, 519, 680
TaiI ACGT 1 cut(s) 621
TaqI TCGA 1 cut(s) 563
TasI AATT 4 cut(s) 324, 585, 701, 712
TauI GCSGC 1 cut(s) 463
TfiI GAWTC 2 cut(s) 239, 481
Tru1I TTAA 6 cut(s) 216, 354, 498, 588, 654, 771
Tru9I TTAA 6 cut(s) 216, 354, 498, 588, 654, 771
TscAI CASTG 3 cut(s) 39, 123, 346
TseFI GTSAC 4 cut(s) 14, 286, 661, 748
TseI GCWGC 4 cut(s) 29, 605, 719, 731
Tsp45I GTSAC 4 cut(s) 14, 286, 661, 748
TspDTI ATGAA 1 cut(s) 186
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 3 cut(s) 39, 123, 346
VpaK11BI GGWCC 1 cut(s) 181
VspI ATTAAT 2 cut(s) 216, 771
XapI RAATTY 2 cut(s) 701, 712
XbaI TCTAGA 1 cut(s) 535
XceI RCATGY 1 cut(s) 396
XmiI GTMKAC 2 cut(s) 521, 675
XmnI GAANNNNTTC 3 cut(s) 111, 128, 238
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.