Rh6BG027400

5'-methylthioadenosine S-adenosylhomocysteine nucleosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010266
Physical Location & Seq
Reverse (-)
4101975 .. 4107487
5513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG027400.1

Sequence Viewer

Length: 612 bp
ATGGCTCCTCACGGCGACAAATCGGACGCTGCGGAGGACGCCATGGTTTCCGGAGCTGAGAAGCGCCCCATATCCTCCGTCCTCTTCGTCATCGCTATGCAGACTGAGGCGCTTCCGGTGGTGAATAAGTTTAACCTCACTGAGGTCCCTGACTCCGGGTTTCCGAAAGGAGTTCCTTGGGTTCGGTATCATGGGTTTTACAAAGATCTTGAGATTAATCTAGTGTGGCCTGGGAAAGATGAATCTTTGGGGGTTGATTGTGTAGGCACAGTTCCTGCATCTCTTGTGACCTATGCTTCCATTCAAGCATTACAGCCAGACCTAATTATCAATGCAGGCACTGCTGGTGGCTTTAAGGACAAAGGAGCATGCATTGGTGACATTTATCTCGCATCTGATGTTGCTTACCATGATAGAAGGATACCTATTCCTGTTTTTGATTTATACGGAGTTGGCTTGCGACTCGCCTACACAACACCCAGTCTTCAAAAGGAACTTGAGCTAAAGGTTGGCAAGCTGTCTACTGGCAACTCTTTGGATATGTCTCCACAGGATGAAGCATCAATAGTTGCAAATGATGCTACCGTTAAAGACATGGAGGTAACCTTTTGA

Protein Analysis

203

Amino Acids

21.81

Weight (kDa)

4.67

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNP_UDP_1 PF01048 28 - 201 9.9e-15 Phosphorylase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34840 AT4G34840 AT4G34840 AT4G34840 AT4G34840 AT4G38800
fragaria_vesca FvH4_2g02150
malus_domestica MD10G1116600.v1.1
prunus_persica Prupe.8G159400_v2.0.a1 Prupe.8G159500_v2.0.a1
pyrus_communis pycom10g10140
rosa_chinensis RchiOBHm_Chr6g0245651
rosa_laevigata RLG00000015296
rosa_multiflora Rmu_sc0016035.1_g000004
rosa_roxburghii Rroxscaffold_7G00215150
rosa_rugosa Rorug05G0515300
rosa_samantha Rh6AG030000 Rh6BG027400 Rh6CG026700 Rh6DG026100
rosa_wichuraiana Rw6G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 521
AccIII TCCGGA 1 cut(s) 50
AciI CCGC 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 39
AfiI CCNNNNNNNGG 2 cut(s) 142, 155
AgsI TTSAA 2 cut(s) 305, 488
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 3 cut(s) 56, 502, 517
AluI AGCT 3 cut(s) 56, 502, 517
Alw26I GTCTC 1 cut(s) 549
AlwNI CAGNNNCTG 2 cut(s) 275, 341
Aor13HI TCCGGA 1 cut(s) 50
AoxI GGCC 1 cut(s) 227
ApeKI GCWGC 1 cut(s) 29
AseI ATTAAT 1 cut(s) 216
AspLEI GCGC 2 cut(s) 66, 112
AspS9I GGNCC 1 cut(s) 145
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 2 cut(s) 133, 389
AvaII GGWCC 1 cut(s) 145
BbsI GAAGAC 1 cut(s) 476
BbvI GCAGC 1 cut(s) 16
BceAI ACGGC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 231
BciVI GTATCC 1 cut(s) 414
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 549
BfaI CTAG 1 cut(s) 221
BfoI RGCGCY 2 cut(s) 67, 113
BfuI GTATCC 1 cut(s) 414
BglII AGATCT 1 cut(s) 205
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 2 cut(s) 157, 231
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 2 cut(s) 6, 147
BmrFI CCNGG 2 cut(s) 157, 231
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 4 cut(s) 287, 401, 568, 569
BmuI ACTGGG 1 cut(s) 474
BpiI GAAGAC 1 cut(s) 476
BpuEI CTTGAG 2 cut(s) 230, 518
BpuMI CCSGG 1 cut(s) 157
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 3 cut(s) 42, 176, 230
BsaWI WCCGGW 2 cut(s) 50, 115
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 155
Bse1I ACTGG 2 cut(s) 480, 529
BseAI TCCGGA 1 cut(s) 50
BseBI CCWGG 1 cut(s) 231
BseDI CCNNGG 3 cut(s) 42, 176, 230
BseGI GGATG 1 cut(s) 559
BseLI CCNNNNNNNGG 2 cut(s) 142, 155
BseMII CTCAG 3 cut(s) 48, 96, 132
BseNI ACTGG 2 cut(s) 480, 529
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 1 cut(s) 229
BsiSI CCGG 3 cut(s) 51, 116, 156
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 142, 155
BsmAI GTCTC 1 cut(s) 549
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 229
Bsp13I TCCGGA 1 cut(s) 50
Bsp143I GATC 1 cut(s) 205
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 1 cut(s) 32
BspANI GGCC 1 cut(s) 229
BspCNI CTCAG 3 cut(s) 49, 97, 133
BspEI TCCGGA 1 cut(s) 50
BspLI GGNNCC 2 cut(s) 6, 147
BsrI ACTGG 2 cut(s) 480, 529
BssECI CCNNGG 3 cut(s) 42, 176, 230
BssMI GATC 1 cut(s) 205
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 2 cut(s) 42, 176
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 2 cut(s) 271, 586
Bst6I CTCTTC 1 cut(s) 89
BstACI GRCGYC 1 cut(s) 39
BstAPI GCANNNNNTGC 2 cut(s) 341, 578
BstC8I GCNNGC 4 cut(s) 337, 370, 458, 515
BstDEI CTNAG 3 cut(s) 57, 105, 141
BstDSI CCRYGG 1 cut(s) 42
BstEII GGTNACC 1 cut(s) 601
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 1 cut(s) 559
BstH2I RGCGCY 2 cut(s) 67, 113
BstHHI GCGC 2 cut(s) 66, 112
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 3 cut(s) 38, 341, 578
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 372
BstPI GGTNACC 1 cut(s) 601
BstSCI CCNGG 2 cut(s) 155, 229
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 476
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BsuI GTATCC 1 cut(s) 414
BsuRI GGCC 1 cut(s) 229
BtgI CCRYGG 1 cut(s) 42
BtgZI GCGATG 1 cut(s) 76
BtsCI GGATG 1 cut(s) 559
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 2 cut(s) 138, 339
Cac8I GCNNGC 4 cut(s) 337, 370, 458, 515
CaiI CAGNNNCTG 2 cut(s) 275, 341
CfoI GCGC 2 cut(s) 66, 112
Cfr13I GGNCC 1 cut(s) 145
CseI GACGC 2 cut(s) 35, 47
CviAII CATG 5 cut(s) 43, 191, 369, 410, 595
CviJI RGCY 8 cut(s) 5, 56, 229, 316, 351, 456, 502, 517
CviKI_1 RGCY 8 cut(s) 5, 56, 229, 316, 351, 456, 502, 517
DdeI CTNAG 3 cut(s) 57, 105, 141
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Eco130I CCWWGG 2 cut(s) 42, 176
Eco47I GGWCC 1 cut(s) 145
Eco91I GGTNACC 1 cut(s) 601
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 145
EcoO65I GGTNACC 1 cut(s) 601
EcoRII CCWGG 1 cut(s) 229
EcoT14I CCWWGG 2 cut(s) 42, 176
EcoT22I ATGCAT 1 cut(s) 374
ErhI CCWWGG 2 cut(s) 42, 176
FaeI CATG 5 cut(s) 46, 194, 372, 413, 598
FaqI GGGAC 1 cut(s) 131
FatI CATG 5 cut(s) 42, 190, 368, 409, 594
FblI GTMKAC 1 cut(s) 521
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 1 cut(s) 566
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 1 cut(s) 221
GlaI GCGC 2 cut(s) 65, 111
GluI GCNGC 1 cut(s) 30
HaeII RGCGCY 2 cut(s) 67, 113
HaeIII GGCC 1 cut(s) 229
HapII CCGG 3 cut(s) 51, 116, 156
HgaI GACGC 2 cut(s) 35, 47
HhaI GCGC 2 cut(s) 66, 112
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 5 cut(s) 46, 194, 372, 413, 598
Hin6I GCGC 2 cut(s) 64, 110
HinP1I GCGC 2 cut(s) 64, 110
HinfI GANTC 3 cut(s) 152, 242, 462
HpaII CCGG 3 cut(s) 51, 116, 156
HphI GGTGA 2 cut(s) 133, 389
Hpy166II GTNNAC 1 cut(s) 522
Hpy188I TCNGA 3 cut(s) 25, 165, 397
Hpy188III TCNNGA 2 cut(s) 51, 209
Hpy8I GTNNAC 1 cut(s) 522
HpyAV CCTTC 1 cut(s) 411
HpyCH4III ACNGT 2 cut(s) 271, 586
HpyCH4V TGCA 5 cut(s) 100, 278, 335, 372, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 341, 578
HpyF3I CTNAG 3 cut(s) 57, 105, 141
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 5 cut(s) 46, 194, 372, 413, 598
HspAI GCGC 2 cut(s) 64, 110
Kpn2I TCCGGA 1 cut(s) 50
Kzo9I GATC 1 cut(s) 205
LmnI GCTCC 3 cut(s) 10, 53, 365
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 4 cut(s) 287, 401, 568, 569
MaeI CTAG 1 cut(s) 221
MaeIII GTNAC 3 cut(s) 286, 377, 601
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 2 cut(s) 76, 476
MflI RGATCY 1 cut(s) 205
MluCI AATT 1 cut(s) 324
MlyI GAGTC 2 cut(s) 146, 456
MnlI CCTC 8 cut(s) 18, 28, 85, 92, 100, 136, 146, 592
Mph1103I ATGCAT 1 cut(s) 374
MroI TCCGGA 1 cut(s) 50
MseI TTAA 4 cut(s) 132, 216, 354, 588
MslI CAYNNNNRTG 1 cut(s) 95
MspI CCGG 3 cut(s) 51, 116, 156
MspR9I CCNGG 2 cut(s) 157, 231
MvaI CCWGG 1 cut(s) 231
MwoI GCNNNNNNNGC 3 cut(s) 38, 341, 578
NciI CCSGG 1 cut(s) 157
NcoI CCATGG 1 cut(s) 42
NdeII GATC 1 cut(s) 205
NlaIII CATG 5 cut(s) 46, 194, 372, 413, 598
NlaIV GGNNCC 2 cut(s) 6, 147
NmuCI GTSAC 2 cut(s) 286, 377
NsiI ATGCAT 1 cut(s) 374
NspI RCATGY 1 cut(s) 372
PaeI GCATGC 1 cut(s) 372
PfeI GAWTC 1 cut(s) 242
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 2 cut(s) 146, 456
PpsI GAGTC 2 cut(s) 146, 456
PpuMI RGGWCCY 1 cut(s) 145
PshBI ATTAAT 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 145
Psp6I CCWGG 1 cut(s) 229
PspEI GGTNACC 1 cut(s) 601
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 2 cut(s) 6, 147
PspPI GGNCC 1 cut(s) 145
PspPPI RGGWCCY 1 cut(s) 145
PsrI GAACNNNNNNTAC 2 cut(s) 255, 287
PstNI CAGNNNCTG 2 cut(s) 275, 341
PsuI RGATCY 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 4 cut(s) 132, 216, 354, 588
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 1 cut(s) 145
SchI GAGTC 2 cut(s) 146, 456
ScrFI CCNGG 2 cut(s) 157, 231
SfaNI GCATC 4 cut(s) 287, 401, 568, 569
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 95
SmlI CTYRAG 2 cut(s) 209, 497
SmoI CTYRAG 2 cut(s) 209, 497
SphI GCATGC 1 cut(s) 372
Sse9I AATT 1 cut(s) 324
SsiI CCGC 1 cut(s) 32
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 2 cut(s) 155, 229
StyI CCWWGG 2 cut(s) 42, 176
TaaI ACNGT 2 cut(s) 271, 586
TasI AATT 1 cut(s) 324
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 4 cut(s) 132, 216, 354, 588
Tru9I TTAA 4 cut(s) 132, 216, 354, 588
TscAI CASTG 2 cut(s) 145, 346
TseFI GTSAC 2 cut(s) 286, 377
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 2 cut(s) 286, 377
TspDTI ATGAA 2 cut(s) 255, 570
TspGWI ACGGA 2 cut(s) 67, 462
TspRI CASTG 2 cut(s) 145, 346
VpaK11BI GGWCC 1 cut(s) 145
VspI ATTAAT 1 cut(s) 216
XagI CCTNNNNNAGG 1 cut(s) 140
XceI RCATGY 1 cut(s) 372
XmiI GTMKAC 1 cut(s) 521
XspI CTAG 1 cut(s) 221
Zsp2I ATGCAT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.