MD10G1138900.v1.1

Transmembrane protein 184 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011477
Physical Location & Seq
Reverse (-)
22375936 .. 22383366
7431 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1138900.v1.1.491

Sequence Viewer

Length: 1461 bp
ATGAAGATAACACCATTTCTCGATCTCATTACCCATTTGGCCTACACACCTCCTACCTGGGCAAGTCTAGCTTGTGGTGTCTTTGTACTCATCACTCTTTCCCTTTCAATTTATCTTGTATTTGAGCACCTTTCTGCATACAAAAATCCTGAGGAGCAAAAGTTTTTAATTGGTGTTGTACTCATGGTTCCGTGCTATGCAATAGAATCTTTAGTATCTTTCTTGAACCCTTCAATAAGCGTTTATTGCGAGATTCTACGGGATTGCTATGAATCCTTTGCCATGTATTGCTTTGGAAGGTACTTGGTTGCATGCTTGGGTGGGGAAGAGAGGACTATTGAGTTTATGGAAAGAGAAGGACGTGCAAGTTCTAAGATTCCCCTATTAGAACACAGTTCTGAAAGGGGAACAGTTAATCATCCTTTTCCGGTGAACTATATCTTGAAACCATGGAAACTTGGTCAATGGTTTTACCAAGTTGTCAAATTCGGAATTGTTCAGTATATGTTAATTAAGTCTCTCAGTGCTATTGTAGCAGTTATCCTTGAAGCTTTTGATGTATATTGCGAAGGTGACTTTAAATGGGGATGTGGGTATATTTATATTGCTGTGGTTCTAAATTTTAGTCAGTCATGGGCTTTGTACTGTCTCGTTCAGTTTTACACTGTTACAAAAGATGGACTAGCTCGCATAAAACCATTAGCCAAGTTTTTGACATTTAAATCAATTGTGTTTTTGACATGGTGGCAAGGTGTGGCAATTGCTCTTCTCTATTCCTTGGGTTTGTTCAAAAGCCCTATAGCTCAAGGATTGCAGTTTAAGTCAAGTGTTCAAGACTTCATCATTTGTATAGAGATGGGCATTGCCTCTGTTGTTCACCTATATGTGTTTCCTGCCAAACCTTATGAAATGATGGGAGATCGTTTTGCTGGAACTGTCTCAGTTCTTGGAGACTATGTATCGGCTGACTGTCCTTTGGATCCGGAGGAGGTTAGGGACAGTGAACGCCCCACAAAGCTACGCCTCCCCACTCCTGACATTGATGTCAAGAGTGGCATGACAATCAGAGAAAGTGTTCGAGATGTTTTCATTGGTGGCGGGGAATATATTGTGCAAGATGTGAAGTTCACAGTTACACAGGCAGTAGAGCCAGTGGAGAAGGGGTTCACCAGGTTCAATGAGAAGCTACACAAGATCTCCGAAAACATTAAGAAACATGACAAGGAGAAACGAAAGACGAGGGATGACAGTTGTATATCCACATCACCTACACGGAAGGTGATTCGTGGGATAGATGACCCTCTCTTGAACGGAAGCATGAGTGACAGTGGGGTTGCAAGGGCAAATAGGCATCGCCGCAAATCTGGATATACAAGTGCAGAAAGCGGTGGAGAGAGCGGTAGTGATCAGAGTTATGGTGGGTTTCAGGTCAGAGGCCGTAGGTGGGTAACCAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Solute_trans_a

Protein Analysis

487

Amino Acids

54.84

Weight (kDa)

8.13

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Solute_trans_a PF03619 23 - 302 2.3e-94 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38360
fragaria_vesca FvH4_2g16560 FvH4_2g16560
malus_domestica MD05G1133900.v1.1 MD10G1138900.v1.1
prunus_persica Prupe.8G177700_v2.0.a1
pyrus_communis pycom05g13070 pycom10g11810
rosa_chinensis RchiOBHm_Chr6g0280631
rosa_laevigata RLG00000013042
rosa_multiflora Rmu_sc0000976.1_g000001
rosa_roxburghii Rroxscaffold_7G00187860
rosa_rugosa Rorug06G0133000 Rorug06G0133100 Rorug06G0133200
rosa_samantha Rh6AG245600 Rh6BG248000 Rh6CG249400 Rh6DG240600
rosa_wichuraiana Rw6G021260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1043
AccBSI CCGCTC 1 cut(s) 1398
AccIII TCCGGA 1 cut(s) 982
AciI CCGC 4 cut(s) 1098, 1357, 1386, 1398
AclWI GGATC 2 cut(s) 974, 987
AcsI RAATTY 2 cut(s) 485, 619
AfaI GTAC 4 cut(s) 87, 180, 302, 644
AfiI CCNNNNNNNGG 1 cut(s) 1444
AgsI TTSAA 9 cut(s) 108, 226, 234, 445, 548, 790, 833, 1177, 1309
AjiI CACGTC 1 cut(s) 362
AjnI CCWGG 2 cut(s) 56, 1169
AloI GAACNNNNNNTCC 2 cut(s) 1148, 1180
AluBI AGCT 6 cut(s) 71, 551, 686, 803, 1018, 1186
AluI AGCT 6 cut(s) 71, 551, 686, 803, 1018, 1186
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 4 cut(s) 522, 653, 943, 945
AlwI GGATC 2 cut(s) 974, 987
Aor13HI TCCGGA 1 cut(s) 982
AoxI GGCC 2 cut(s) 39, 1435
ApoI RAATTY 2 cut(s) 485, 619
Asp700I GAANNNNTTC 2 cut(s) 1074, 1163
AsuHPI GGTGA 6 cut(s) 442, 584, 869, 1159, 1257, 1291
AxyI CCTNAGG 1 cut(s) 150
BamHI GGATCC 1 cut(s) 979
Bbv12I GWGCWC 1 cut(s) 129
BccI CCATC 3 cut(s) 671, 850, 907
BceAI ACGGC 1 cut(s) 1422
BciT130I CCWGG 2 cut(s) 58, 1171
BclI TGATCA 1 cut(s) 1405
BcoDI GTCTC 4 cut(s) 522, 653, 943, 945
BfaI CTAG 2 cut(s) 68, 683
BfmI CTRYAG 1 cut(s) 798
BglII AGATCT 1 cut(s) 1194
BisI GCNGC 1 cut(s) 1357
BlsI GCNGC 1 cut(s) 1358
Bme1390I CCNGG 2 cut(s) 58, 1171
BmgBI CACGTC 1 cut(s) 362
BmiI GGNNCC 2 cut(s) 189, 981
BmrFI CCNGG 2 cut(s) 58, 1171
BmsI GCATC 1 cut(s) 1360
BpuEI CTTGAG 1 cut(s) 789
BsaJI CCNNGG 3 cut(s) 57, 449, 777
BsaWI WCCGGW 2 cut(s) 427, 982
BsaXI ACNNNNNCTCC 8 cut(s) 146, 176, 942, 972, 1148, 1178, 1181, 1211
Bsc4I CCNNNNNNNGG 1 cut(s) 1444
Bse1I ACTGG 1 cut(s) 1151
Bse21I CCTNAGG 1 cut(s) 150
Bse3DI GCAATG 1 cut(s) 861
BseAI TCCGGA 1 cut(s) 982
BseBI CCWGG 2 cut(s) 58, 1171
BseDI CCNNGG 3 cut(s) 57, 449, 777
BseGI GGATG 3 cut(s) 418, 593, 1249
BseLI CCNNNNNNNGG 1 cut(s) 1444
BseMI GCAATG 1 cut(s) 861
BseMII CTCAG 3 cut(s) 141, 535, 954
BseNI ACTGG 1 cut(s) 1151
BseRI GAGGAG 2 cut(s) 167, 1001
BsgI GTGCAG 1 cut(s) 1398
BshFI GGCC 2 cut(s) 41, 1437
BsiHKAI GWGCWC 1 cut(s) 129
BsiSI CCGG 2 cut(s) 428, 983
BslFI GGGAC 1 cut(s) 1010
BslI CCNNNNNNNGG 1 cut(s) 1444
BsmAI GTCTC 4 cut(s) 522, 653, 943, 945
BsmFI GGGAC 1 cut(s) 1010
BsnI GGCC 2 cut(s) 41, 1437
Bsp1286I GDGCHC 1 cut(s) 129
Bsp13I TCCGGA 1 cut(s) 982
Bsp143I GATC 5 cut(s) 22, 919, 979, 1194, 1405
Bsp19I CCATGG 1 cut(s) 449
BspACI CCGC 4 cut(s) 1098, 1357, 1386, 1398
BspANI GGCC 2 cut(s) 41, 1437
BspCNI CTCAG 3 cut(s) 142, 534, 953
BspEI TCCGGA 1 cut(s) 982
BspLI GGNNCC 2 cut(s) 189, 981
BspPI GGATC 2 cut(s) 974, 987
BspQI GCTCTTC 1 cut(s) 771
BsrBI CCGCTC 1 cut(s) 1398
BsrDI GCAATG 1 cut(s) 861
BsrI ACTGG 1 cut(s) 1151
BssECI CCNNGG 3 cut(s) 57, 449, 777
BssMI GATC 5 cut(s) 22, 919, 979, 1194, 1405
BssT1I CCWWGG 2 cut(s) 449, 777
Bst2UI CCWGG 2 cut(s) 58, 1171
Bst6I CTCTTC 2 cut(s) 321, 771
BstC8I GCNNGC 2 cut(s) 313, 688
BstDEI CTNAG 4 cut(s) 150, 372, 521, 940
BstDSI CCRYGG 1 cut(s) 449
BstEII GGTNACC 1 cut(s) 1447
BstF5I GGATG 3 cut(s) 418, 593, 1249
BstKTI GATC 5 cut(s) 25, 922, 982, 1197, 1408
BstMAI GTCTC 4 cut(s) 522, 653, 943, 945
BstMBI GATC 5 cut(s) 22, 919, 979, 1194, 1405
BstMWI GCNNNNNNNGC 3 cut(s) 68, 246, 533
BstNI CCWGG 2 cut(s) 58, 1171
BstNSI RCATGY 1 cut(s) 315
BstPI GGTNACC 1 cut(s) 1447
BstSCI CCNGG 2 cut(s) 56, 1169
BstSFI CTRYAG 1 cut(s) 798
BstX2I RGATCY 2 cut(s) 979, 1194
BstYI RGATCY 2 cut(s) 979, 1194
Bsu36I CCTNAGG 1 cut(s) 150
BsuRI GGCC 2 cut(s) 41, 1437
BtgI CCRYGG 1 cut(s) 449
BtgZI GCGATG 1 cut(s) 1337
BtrI CACGTC 1 cut(s) 362
BtsCI GGATG 3 cut(s) 418, 593, 1249
BtsIMutI CAGTG 5 cut(s) 529, 663, 1006, 1158, 1333
Cac8I GCNNGC 2 cut(s) 313, 688
CsiI ACCWGGT 1 cut(s) 1169
Csp6I GTAC 4 cut(s) 86, 179, 301, 643
CviAII CATG 9 cut(s) 184, 283, 312, 450, 633, 741, 1057, 1217, 1318
CviQI GTAC 4 cut(s) 86, 179, 301, 643
DdeI CTNAG 4 cut(s) 150, 372, 521, 940
DpnI GATC 5 cut(s) 24, 921, 981, 1196, 1407
DpnII GATC 5 cut(s) 22, 919, 979, 1194, 1405
DraI TTTAAA 2 cut(s) 580, 721
DrdI GACNNNNNNGTC 1 cut(s) 1043
DseDI GACNNNNNNGTC 1 cut(s) 1043
Eam1104I CTCTTC 2 cut(s) 321, 771
EarI CTCTTC 2 cut(s) 321, 771
Eco130I CCWWGG 2 cut(s) 449, 777
Eco81I CCTNAGG 1 cut(s) 150
Eco91I GGTNACC 1 cut(s) 1447
EcoO65I GGTNACC 1 cut(s) 1447
EcoRII CCWGG 2 cut(s) 56, 1169
EcoT14I CCWWGG 2 cut(s) 449, 777
ErhI CCWWGG 2 cut(s) 449, 777
FaeI CATG 9 cut(s) 187, 286, 315, 453, 636, 744, 1060, 1220, 1321
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FaqI GGGAC 1 cut(s) 1010
FatI CATG 9 cut(s) 183, 282, 311, 449, 632, 740, 1056, 1216, 1317
FauI CCCGC 1 cut(s) 1091
FbaI TGATCA 1 cut(s) 1405
Fnu4HI GCNGC 1 cut(s) 1357
FokI GGATG 3 cut(s) 405, 600, 1256
Fsp4HI GCNGC 1 cut(s) 1357
FspBI CTAG 2 cut(s) 68, 683
GluI GCNGC 1 cut(s) 1357
HaeIII GGCC 2 cut(s) 41, 1437
HapII CCGG 2 cut(s) 428, 983
Hin1II CATG 9 cut(s) 187, 286, 315, 453, 636, 744, 1060, 1220, 1321
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 5 cut(s) 206, 253, 272, 376, 1282
HpaII CCGG 2 cut(s) 428, 983
HphI GGTGA 6 cut(s) 442, 584, 869, 1159, 1257, 1291
Hpy166II GTNNAC 5 cut(s) 433, 877, 1004, 1128, 1167
Hpy188I TCNGA 6 cut(s) 400, 491, 1067, 1201, 1410, 1433
Hpy8I GTNNAC 5 cut(s) 433, 877, 1004, 1128, 1167
HpyAV CCTTC 6 cut(s) 240, 291, 350, 563, 1153, 1270
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 8 cut(s) 137, 200, 311, 365, 814, 1114, 1337, 1379
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 246, 533
HpyF3I CTNAG 4 cut(s) 150, 372, 521, 940
HpySE526I ACGT 1 cut(s) 361
Hsp92II CATG 9 cut(s) 187, 286, 315, 453, 636, 744, 1060, 1220, 1321
Kpn2I TCCGGA 1 cut(s) 982
Ksp22I TGATCA 1 cut(s) 1405
Kzo9I GATC 5 cut(s) 22, 919, 979, 1194, 1405
LguI GCTCTTC 1 cut(s) 771
LmnI GCTCC 1 cut(s) 154
LweI GCATC 1 cut(s) 1360
MabI ACCWGGT 1 cut(s) 1169
MaeI CTAG 2 cut(s) 68, 683
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 5 cut(s) 572, 667, 1132, 1322, 1447
MalI GATC 5 cut(s) 24, 921, 981, 1196, 1407
MbiI CCGCTC 1 cut(s) 1398
MboI GATC 5 cut(s) 22, 919, 979, 1194, 1405
MboII GAAGA 3 cut(s) 16, 338, 758
MfeI CAATTG 2 cut(s) 726, 759
MflI RGATCY 2 cut(s) 979, 1194
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 8 cut(s) 108, 168, 485, 492, 510, 619, 726, 759
MroI TCCGGA 1 cut(s) 982
MroXI GAANNNNTTC 2 cut(s) 1074, 1163
MseI TTAA 8 cut(s) 167, 414, 509, 513, 579, 720, 819, 1209
MslI CAYNNNNRTG 1 cut(s) 882
MspI CCGG 2 cut(s) 428, 983
MspR9I CCNGG 2 cut(s) 58, 1171
MunI CAATTG 2 cut(s) 726, 759
MvaI CCWGG 2 cut(s) 58, 1171
MwoI GCNNNNNNNGC 3 cut(s) 68, 246, 533
NcoI CCATGG 1 cut(s) 449
NdeII GATC 5 cut(s) 22, 919, 979, 1194, 1405
NlaIII CATG 9 cut(s) 187, 286, 315, 453, 636, 744, 1060, 1220, 1321
NlaIV GGNNCC 2 cut(s) 189, 981
NmuCI GTSAC 2 cut(s) 572, 1322
NspI RCATGY 1 cut(s) 315
PacI TTAATTAA 1 cut(s) 513
PaeI GCATGC 1 cut(s) 315
PciSI GCTCTTC 1 cut(s) 771
PdmI GAANNNNTTC 2 cut(s) 1074, 1163
PfeI GAWTC 5 cut(s) 206, 253, 272, 376, 1282
PkrI GCNGC 1 cut(s) 1358
Psp6I CCWGG 2 cut(s) 56, 1169
PspEI GGTNACC 1 cut(s) 1447
PspGI CCWGG 2 cut(s) 56, 1169
PspN4I GGNNCC 2 cut(s) 189, 981
PsrI GAACNNNNNNTAC 2 cut(s) 171, 203
PsuI RGATCY 2 cut(s) 979, 1194
RsaI GTAC 4 cut(s) 87, 180, 302, 644
RsaNI GTAC 4 cut(s) 86, 179, 301, 643
RseI CAYNNNNRTG 1 cut(s) 882
SapI GCTCTTC 1 cut(s) 771
SaqAI TTAA 8 cut(s) 167, 414, 509, 513, 579, 720, 819, 1209
SatI GCNGC 1 cut(s) 1357
Sau3AI GATC 5 cut(s) 22, 919, 979, 1194, 1405
ScrFI CCNGG 2 cut(s) 58, 1171
SduI GDGCHC 1 cut(s) 129
SexAI ACCWGGT 1 cut(s) 1169
SfaNI GCATC 1 cut(s) 1360
SfcI CTRYAG 1 cut(s) 798
SmiI ATTTAAAT 1 cut(s) 721
SmiMI CAYNNNNRTG 1 cut(s) 882
SmlI CTYRAG 1 cut(s) 804
SmoI CTYRAG 1 cut(s) 804
SphI GCATGC 1 cut(s) 315
Sse9I AATT 8 cut(s) 108, 168, 485, 492, 510, 619, 726, 759
SsiI CCGC 4 cut(s) 1098, 1357, 1386, 1398
SspMI CTAG 2 cut(s) 68, 683
StyD4I CCNGG 2 cut(s) 56, 1169
StyI CCWWGG 2 cut(s) 449, 777
SwaI ATTTAAAT 1 cut(s) 721
TaiI ACGT 1 cut(s) 364
TaqI TCGA 2 cut(s) 21, 1078
TasI AATT 8 cut(s) 108, 168, 485, 492, 510, 619, 726, 759
TatI WGTACW 3 cut(s) 85, 178, 642
TauI GCSGC 1 cut(s) 1359
TfiI GAWTC 5 cut(s) 206, 253, 272, 376, 1282
Tru1I TTAA 8 cut(s) 167, 414, 509, 513, 579, 720, 819, 1209
Tru9I TTAA 8 cut(s) 167, 414, 509, 513, 579, 720, 819, 1209
TscAI CASTG 5 cut(s) 529, 670, 1006, 1158, 1333
TseFI GTSAC 2 cut(s) 572, 1322
Tsp45I GTSAC 2 cut(s) 572, 1322
TspDTI ATGAA 5 cut(s) 17, 285, 829, 921, 1078
TspGWI ACGGA 3 cut(s) 180, 1288, 1326
TspRI CASTG 5 cut(s) 529, 670, 1006, 1158, 1333
XapI RAATTY 2 cut(s) 485, 619
XceI RCATGY 1 cut(s) 315
XmnI GAANNNNTTC 2 cut(s) 1074, 1163
XspI CTAG 2 cut(s) 68, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.