Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007602

Physical Location & Seq

Genomic Coordinates Forward (+)

25657292 .. 25661119

Gene Structure 3828 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1166000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 660 bp

ATGGGGCAGCAATCGTTGATCTACAGCTTCGTAGCGCGCGGCACGGTGGTCCTCGCAGAGTACACGGAATTCACCGGAAACTTCACCAGCATAGCCTCCCAGTGCCTCCAGAAACTTCCGGCCACCAACAACAAGTTCACCTACAACTGCGACGGCCACACCTTCAACTACCTCGTCGATAATGGCTTCACATATTGTGTAGTCGCAGTTGAGGCGGTTGGTCGACAAGTTCCTATTGCCTTCCTTGAGCGGATCAAGGAGGATTTTACTGGTAGATATGGTGGGGGAAAAGCTGCAACAGCAGTTGCAAATAGCCTGAACAAGGAATTTGGGTCCAAACTGAAGGAGCACATGCAATACTGTGTGGATCATCCCGAAGAGATCAGCAAGCTTGCAAAAGTGAAAGCTCAGGTTTCCGAAGTCAAGGGAGTTATGATGGAAAATATTGAGAAGGTTCTTGATCGTGGGGAGAAGATTGAGCTTCTGGTGGATAAAACAGAGAACCTCCGCTCACAGGCACAAGATTTCAGGCAGCAGGGGACCCAGATGAGGAGGAAGATGTGGTTGCAGAACATGAAGGTGAAGCTGATAGTTTTGGGAATCTTAATCGCATTGATTCTCATCATCGTTCTATCCGTGTGCAATGGCTTCAAATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031201 GO:0031410 GO:0031982 GO:0032940 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048284 GO:0051179 GO:0051234 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0097708 GO:0098796

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Longin Synaptobrevin

Protein Analysis

220

Amino Acids

24.57

Weight (kDa)

8.83

Isoelectric Point (pI)

29.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Longin	PF13774	32 - 111	1.2e-21	Regulated-SNARE-like domain
Synaptobrevin	PF00957	130 - 210	2.9e-30	Synaptobrevin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007602)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33110
fragaria_vesca	FvH4_2g19280 FvH4_3g12050
malus_domestica	MD05G1177800.v1.1 MD10G1166000.v1.1
prunus_persica	Prupe.8G204500_v2.0.a1 Prupe.8G204500_v2.0.a1
pyrus_communis	pycom05g16420 pycom10g14320
rosa_chinensis	RchiOBHm_Chr6g0285071
rosa_laevigata	RLG00000012680
rosa_multiflora	Rmu_sc0002478.1_g000007 Rmu_sc0002535.1_g000048
rosa_roxburghii	Rroxscaffold_7G00183760 Rroxscaffold_7G00184050
rosa_rugosa	Rorug05G0053700 Rorug06G0170400 Rorug06G0170400 Rorug06G0170500
rosa_samantha	Rh5CG154700 Rh5DG143200 Rh6AG282200 Rh6BG282700
rosa_wichuraiana	Rw6G024240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	250, 510
AccI	GTMKAC	1 cut(s)	223
AccII	CGCG	2 cut(s)	37, 39
AciI	CCGC	4 cut(s)	39, 215, 250, 508
AclWI	GGATC	2 cut(s)	260, 375
AcoI	YGGCCR	2 cut(s)	120, 154
AcsI	RAATTY	2 cut(s)	68, 326
AcuI	CTGAAG	1 cut(s)	362
AfaI	GTAC	1 cut(s)	62
AfiI	CCNNNNNNNGG	3 cut(s)	322, 514, 549
AgsI	TTSAA	2 cut(s)	166, 652
AjuI	GAANNNNNNNTTGG	2 cut(s)	119, 151
AluBI	AGCT	6 cut(s)	27, 293, 391, 407, 481, 586
AluI	AGCT	6 cut(s)	27, 293, 391, 407, 481, 586
Alw21I	GWGCWC	1 cut(s)	351
AlwI	GGATC	2 cut(s)	260, 375
AoxI	GGCC	2 cut(s)	120, 154
ApeKI	GCWGC	3 cut(s)	7, 293, 532
ApoI	RAATTY	2 cut(s)	68, 326
AspLEI	GCGC	2 cut(s)	37, 39
AspS9I	GGNCC	3 cut(s)	49, 333, 540
AsuHPI	GGTGA	4 cut(s)	64, 76, 130, 592
AvaII	GGWCC	3 cut(s)	49, 333, 540
Bbv12I	GWGCWC	1 cut(s)	351
BbvI	GCAGC	3 cut(s)	19, 280, 544
BccI	CCATC	1 cut(s)	430
BceAI	ACGGC	1 cut(s)	169
BfmI	CTRYAG	1 cut(s)	22
BisI	GCNGC	4 cut(s)	8, 40, 294, 533
BlsI	GCNGC	4 cut(s)	9, 41, 295, 534
Bme18I	GGWCC	3 cut(s)	49, 333, 540
BmgT120I	GGNCC	3 cut(s)	49, 333, 540
BmiI	GGNNCC	3 cut(s)	334, 541, 542
BmrI	ACTGGG	1 cut(s)	94
BmuI	ACTGGG	1 cut(s)	94
BpmI	CTGGAG	1 cut(s)	92
Bpu10I	CCTNAGC	1 cut(s)	408
BpuEI	CTTGAG	1 cut(s)	266
BsaBI	GATNNNNATC	1 cut(s)	620
BsaWI	WCCGGW	1 cut(s)	74
Bsc4I	CCNNNNNNNGG	3 cut(s)	322, 514, 549
Bse1I	ACTGG	2 cut(s)	100, 274
Bse3DI	GCAATG	1 cut(s)	649
Bse8I	GATNNNNATC	1 cut(s)	620
BseGI	GGATG	1 cut(s)	370
BseJI	GATNNNNATC	1 cut(s)	620
BseLI	CCNNNNNNNGG	3 cut(s)	322, 514, 549
BseMI	GCAATG	1 cut(s)	649
BseMII	CTCAG	1 cut(s)	422
BseNI	ACTGG	2 cut(s)	100, 274
BsePI	GCGCGC	1 cut(s)	35
BseRI	GAGGAG	1 cut(s)	565
BseXI	GCAGC	3 cut(s)	19, 280, 544
Bsh1236I	CGCG	2 cut(s)	37, 39
BshFI	GGCC	2 cut(s)	122, 156
BsiHKAI	GWGCWC	1 cut(s)	351
BsiSI	CCGG	2 cut(s)	75, 119
BslFI	GGGAC	1 cut(s)	553
BslI	CCNNNNNNNGG	3 cut(s)	322, 514, 549
BsmFI	GGGAC	1 cut(s)	553
BsnI	GGCC	2 cut(s)	122, 156
Bsp1286I	GDGCHC	1 cut(s)	351
Bsp143I	GATC	5 cut(s)	18, 252, 367, 381, 460
BspACI	CCGC	4 cut(s)	39, 215, 250, 508
BspANI	GGCC	2 cut(s)	122, 156
BspCNI	CTCAG	1 cut(s)	421
BspFNI	CGCG	2 cut(s)	37, 39
BspLI	GGNNCC	3 cut(s)	334, 541, 542
BspPI	GGATC	2 cut(s)	260, 375
BsrBI	CCGCTC	2 cut(s)	250, 510
BsrDI	GCAATG	1 cut(s)	649
BsrI	ACTGG	2 cut(s)	100, 274
BssHII	GCGCGC	1 cut(s)	35
BssMI	GATC	5 cut(s)	18, 252, 367, 381, 460
Bst4CI	ACNGT	2 cut(s)	46, 362
Bst6I	CTCTTC	1 cut(s)	372
BstC8I	GCNNGC	3 cut(s)	37, 389, 393
BstDEI	CTNAG	1 cut(s)	408
BstENI	CCTNNNNNAGG	1 cut(s)	320
BstF5I	GGATG	1 cut(s)	370
BstFNI	CGCG	2 cut(s)	37, 39
BstHHI	GCGC	2 cut(s)	37, 39
BstKTI	GATC	5 cut(s)	21, 255, 370, 384, 463
BstMBI	GATC	5 cut(s)	18, 252, 367, 381, 460
BstMWI	GCNNNNNNNGC	3 cut(s)	212, 299, 654
BstNSI	RCATGY	1 cut(s)	355
BstSFI	CTRYAG	1 cut(s)	22
BstUI	CGCG	2 cut(s)	37, 39
BstV1I	GCAGC	3 cut(s)	19, 280, 544
BsuRI	GGCC	2 cut(s)	122, 156
BtsCI	GGATG	1 cut(s)	370
BtsIMutI	CAGTG	1 cut(s)	107
Cac8I	GCNNGC	3 cut(s)	37, 389, 393
CfoI	GCGC	2 cut(s)	37, 39
Cfr13I	GGNCC	3 cut(s)	49, 333, 540
Csp6I	GTAC	1 cut(s)	61
CviAII	CATG	2 cut(s)	352, 574
CviQI	GTAC	1 cut(s)	61
DdeI	CTNAG	1 cut(s)	408
DpnI	GATC	5 cut(s)	20, 254, 369, 383, 462
DpnII	GATC	5 cut(s)	18, 252, 367, 381, 460
EaeI	YGGCCR	2 cut(s)	120, 154
Eam1104I	CTCTTC	1 cut(s)	372
EarI	CTCTTC	1 cut(s)	372
Eco47I	GGWCC	3 cut(s)	49, 333, 540
Eco57I	CTGAAG	1 cut(s)	362
EcoNI	CCTNNNNNAGG	1 cut(s)	320
EcoO109I	RGGNCCY	1 cut(s)	540
EcoRI	GAATTC	1 cut(s)	68
FaeI	CATG	2 cut(s)	355, 577
FaiI	YATR	6 cut(s)	92, 193, 279, 353, 434, 575
FaqI	GGGAC	1 cut(s)	553
FatI	CATG	2 cut(s)	351, 573
FblI	GTMKAC	1 cut(s)	223
Fnu4HI	GCNGC	4 cut(s)	8, 40, 294, 533
FokI	GGATG	1 cut(s)	357
Fsp4HI	GCNGC	4 cut(s)	8, 40, 294, 533
GlaI	GCGC	2 cut(s)	36, 38
GluI	GCNGC	4 cut(s)	8, 40, 294, 533
GsuI	CTGGAG	1 cut(s)	92
HaeIII	GGCC	2 cut(s)	122, 156
HapII	CCGG	2 cut(s)	75, 119
HhaI	GCGC	2 cut(s)	37, 39
Hin1II	CATG	2 cut(s)	355, 577
Hin6I	GCGC	2 cut(s)	35, 37
HinP1I	GCGC	2 cut(s)	35, 37
HincII	GTYRAC	1 cut(s)	224
HindII	GTYRAC	1 cut(s)	224
HindIII	AAGCTT	1 cut(s)	389
HinfI	GANTC	2 cut(s)	600, 616
HpaII	CCGG	2 cut(s)	75, 119
HphI	GGTGA	4 cut(s)	64, 76, 130, 592
Hpy166II	GTNNAC	3 cut(s)	63, 138, 224
Hpy188I	TCNGA	1 cut(s)	418
Hpy188III	TCNNGA	3 cut(s)	109, 374, 458
Hpy8I	GTNNAC	3 cut(s)	63, 138, 224
Hpy99I	CGWCG	2 cut(s)	155, 179
HpyAV	CCTTC	5 cut(s)	172, 250, 337, 445, 571
HpyCH4III	ACNGT	2 cut(s)	46, 362
HpyCH4V	TGCA	6 cut(s)	296, 308, 355, 395, 568, 642
HpyF10VI	GCNNNNNNNGC	3 cut(s)	212, 299, 654
HpyF3I	CTNAG	1 cut(s)	408
Hsp92II	CATG	2 cut(s)	355, 577
HspAI	GCGC	2 cut(s)	35, 37
KflI	GGGWCCC	1 cut(s)	540
Kzo9I	GATC	5 cut(s)	18, 252, 367, 381, 460
LmnI	GCTCC	1 cut(s)	346
Lsp1109I	GCAGC	3 cut(s)	19, 280, 544
MalI	GATC	5 cut(s)	20, 254, 369, 383, 462
MbiI	CCGCTC	2 cut(s)	250, 510
MboI	GATC	5 cut(s)	18, 252, 367, 381, 460
MboII	GAAGA	3 cut(s)	389, 484, 568
MhlI	GDGCHC	1 cut(s)	351
MluCI	AATT	2 cut(s)	68, 326
MnlI	CCTC	9 cut(s)	62, 106, 116, 182, 205, 253, 515, 543, 546
MseI	TTAA	1 cut(s)	605
MslI	CAYNNNNRTG	1 cut(s)	578
MspI	CCGG	2 cut(s)	75, 119
MvnI	CGCG	2 cut(s)	37, 39
MwoI	GCNNNNNNNGC	3 cut(s)	212, 299, 654
NdeII	GATC	5 cut(s)	18, 252, 367, 381, 460
NlaIII	CATG	2 cut(s)	355, 577
NlaIV	GGNNCC	3 cut(s)	334, 541, 542
NspI	RCATGY	1 cut(s)	355
PauI	GCGCGC	1 cut(s)	35
PcsI	WCGNNNNNNNCGW	1 cut(s)	633
PfeI	GAWTC	2 cut(s)	600, 616
PkrI	GCNGC	4 cut(s)	9, 41, 295, 534
PpuMI	RGGWCCY	1 cut(s)	540
Psp5II	RGGWCCY	1 cut(s)	540
PspN4I	GGNNCC	3 cut(s)	334, 541, 542
PspPI	GGNCC	3 cut(s)	49, 333, 540
PspPPI	RGGWCCY	1 cut(s)	540
PteI	GCGCGC	1 cut(s)	35
RsaI	GTAC	1 cut(s)	62
RsaNI	GTAC	1 cut(s)	61
RseI	CAYNNNNRTG	1 cut(s)	578
SalI	GTCGAC	1 cut(s)	222
SaqAI	TTAA	1 cut(s)	605
SatI	GCNGC	4 cut(s)	8, 40, 294, 533
Sau3AI	GATC	5 cut(s)	18, 252, 367, 381, 460
Sau96I	GGNCC	3 cut(s)	49, 333, 540
SduI	GDGCHC	1 cut(s)	351
SfcI	CTRYAG	1 cut(s)	22
SinI	GGWCC	3 cut(s)	49, 333, 540
SmiMI	CAYNNNNRTG	1 cut(s)	578
SmlI	CTYRAG	1 cut(s)	245
SmoI	CTYRAG	1 cut(s)	245
Sse9I	AATT	2 cut(s)	68, 326
SsiI	CCGC	4 cut(s)	39, 215, 250, 508
SspI	AATATT	1 cut(s)	445
TaaI	ACNGT	2 cut(s)	46, 362
TaqI	TCGA	2 cut(s)	177, 223
TasI	AATT	2 cut(s)	68, 326
TatI	WGTACW	1 cut(s)	60
TauI	GCSGC	1 cut(s)	42
TfiI	GAWTC	2 cut(s)	600, 616
Tru1I	TTAA	1 cut(s)	605
Tru9I	TTAA	1 cut(s)	605
TscAI	CASTG	1 cut(s)	107
TseI	GCWGC	3 cut(s)	7, 293, 532
TspDTI	ATGAA	1 cut(s)	590
TspGWI	ACGGA	2 cut(s)	80, 625
TspRI	CASTG	1 cut(s)	107
VpaK11BI	GGWCC	3 cut(s)	49, 333, 540
XagI	CCTNNNNNAGG	1 cut(s)	320
XapI	RAATTY	2 cut(s)	68, 326
XceI	RCATGY	1 cut(s)	355
XmiI	GTMKAC	1 cut(s)	223

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.