Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0007602

Physical Location & Seq

Genomic Coordinates Reverse (-)

4658191 .. 4665049

Gene Structure 6859 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0053700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGATTGTGAGGACCTACGGCCGGCGTAAGGGAGGAGGAGTAGGCCTTCCCAGGACTTATTCCGACTCAACCCTAAACGACGCCGTTCGCGGCGACGACGACGACGACCTCAGCACCGACCCGTTTAGAATCTCTCTCTCCCAAGATACCCCCCAATTCAATTTCAATTTCTCGTCGTCGCAAGACTCGTCGTCGCAGTGGTCGCAGTTCGATTCGGAGCCCTATCCTCCCGAATTGAAAAGGCCCAGTTCGCGAAACGGCGACGTTTTGAGGAGGCCGTCGAAGAAGGCGAAGAAGGCCGCGGCGGCGCTGGCGACCGCGACGTTGATGGAGGCGCAGGAGTTCGGGGAGATGATGGAGCATGTGGACGAGGTCAATTTCGCGCTAGATGGACTGAGGAATGGTCAGCCGGTTCGGATCAGAAGGGCGAGCTTGTTGTCTTTGTTGGGGATTTGCGGCACGCCTCAGCAGAGACGGCTTCTGAGGACTCAGGGGATGGCAAAAACGATAATTGACGCTATATTGGGTCTCAGTTTTGATGATACACCTAGTGATCTGGCTGCTGCAACAATTTTCTATGTTTTGACTTGTGATGGTCAGGATGATCCTCTTTTGGAATCACCAAGCTGTATTAAATTTTTGATTAGGTTTTGCAAACCAATTGTCTCAAACATCACTGAAGATAAAGCACCAAAAATTGGACGAAAGCTTTTAGCATTGCGTTTGAGTGCTGACATCTCGCATTGTGCACCAAAAAGATTAGACTCCAGCTCAGCTGCTATTCTTTCCAAAGTACATGAGATTCTTGTTACTTGCAAGGAAATGAAACCAAGTTCTGCTGATGATGGTGAAATGAGCATGCCGGAGTTAAGCCCCAAATGGATAGCTTTGCTTACTATGGAGAAAGCTTGCTTATCTACTATTTCTCTTGAAGAAACAACTGGTACAGTGAGGAAGGCTGGGGGCAACTTTAAAGAAAAATTACGAGAGCTTGGAGGCCTTGATGCTGTCTTTGAGGTGTCCATGACTTGCCATTCCGAAATGGAGGGTTGGCTAAAAGGTAATTCTCCTTCTACTTGGGAAAAGGAAATCAACATGGTGCGGAGCCTGGTTCTGCTTTTGAAATGCTTGAAGATCATGGAAAATGCCACATTCTTGAGTAAAGAAAATCAGAGCCACTTGCTAGAAATGAAAGGAAAGTTGGATCCCATGGAAAAGCCCATGTCTTTCACAGAACTTGTTATAAGTACCATCAGTATCCTATCAGGGCTTTATCTACATAAAAGTTTATCTGCTGCCTCCAATGATGACAAGTCTCATAATCTTTCAAATGGGAATGAATATGCTTCTGAGAAAAGCTCCCACAAATGTCAGAGCAATGGATTGGTGTCCACTTCTCGCTCGGTATATTCTGTATCCAGTTCAGAAACTACCAGCACGTCTATGACTGACACCTATTCGTTGAAGACAAGACTTAGTTCTTCCAGAAATGGCTCATCTAGTGGTACAGCAAGCCATTTAAGTGAGGCAACCCGCACATTTAGCTATGGTTCCCGGAAAGATGCTGGTCCCAGTCAGAGATCCTATATTTCTAAAAACTCTATAATTGATCTTTCGGAGGAGAGCCAAGATCCTTTTGCATTTAGCTATGGTTCCGGTGAGGATGCTGGTCTCAGTCAGAGATCCTATATTTCTGAAGACTGTAAAGTTGACCTTTCACAGGAGAGTCAAGATCCTTTTGCATTTGATGAAGATGATATTGAACCTTCTCAGTGGGACATATTATCTGGCAAGAAAAAGATTTGTCGAACGCGAAAAAATGGAGAAGCTTACAGAGAACTTGATGATGGTTGCCAATTGCAGCTGATTATGAGCCAAGCAGAATCAAGTATCGGGGAAGACCATGATATGCCTGAGACATCCTATGCGGGTGCTGTTAGTAAAGAGGGTTCCAGTCTTTTAGCTGACTGCCTTCTTACTGCTGTTAAGGTTCTGATGAACTTGGCAAATGAGAATCCTGTTGGTTGTCAGCAAATTGCTGCGAATGGAGGACTGGAGACCATGTCTTCCTTGATTGCTAGCCACTTTCCTTCATTCTCATCTCCCTTCAGCGAGAGAAATGACAATACTTCAAGCATTGAACTGGATAATCAGAATGGTAGACATCTTACTGATCAAGAGCTAGATTTTCTTGTAGCTATTTTGGGCCTGCTTGTAAACTTAGTTGAGAAAGATGGTCAAAACAGATCACGGCTCGCTGCAGTCACTGTTCACTTACCTATTTCTGATGGGTTTGAAGAGGAAAGTCACAAGGATCTAATTCTGCTAATATGCTCTATATTCCTGGCTAATCAAGGTGCTGGCGAGGGATCCGAAGAAGGAAAAACTTTGCCAAATGATGAGGCAGCTGTGTTACAAGGAGAACAAGAAGCTGAGAAAATGATAGTAGAGGCTTATGCTGCCCTGCTTTTAGCATTCCTTTCAACTGAAAGTAAGGGGGTACGCGATGCCATTGCTGATTGTCTCCCAGATCGTAACCTGGCTATCCTAGTACCTGTGTTGGACAGATTTGTGGCATTTCACTTGACATTAAACATGATCTCACCGGAGACTCATAAAGTCGTAAGCGAAGTAATTGAATCATGTAGGATACGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031201 GO:0031410 GO:0031982 GO:0032940 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048284 GO:0051179 GO:0051234 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0097708 GO:0098796

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Longin Synaptobrevin

Protein Analysis

885

Amino Acids

96.75

Weight (kDa)

5.05

Isoelectric Point (pI)

52.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WAPL	PF07814	108 - 746	4.3e-51	Wings apart-like protein regulation of heterochromatin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007602)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33110
fragaria_vesca	FvH4_2g19280 FvH4_3g12050
malus_domestica	MD05G1177800.v1.1 MD10G1166000.v1.1
prunus_persica	Prupe.8G204500_v2.0.a1 Prupe.8G204500_v2.0.a1
pyrus_communis	pycom05g16420 pycom10g14320
rosa_chinensis	RchiOBHm_Chr6g0285071
rosa_laevigata	RLG00000012680
rosa_multiflora	Rmu_sc0002478.1_g000007 Rmu_sc0002535.1_g000048
rosa_roxburghii	Rroxscaffold_7G00183760 Rroxscaffold_7G00184050
rosa_rugosa	Rorug05G0053700 Rorug06G0170400 Rorug06G0170400 Rorug06G0170500
rosa_samantha	Rh5CG154700 Rh5DG143200 Rh6AG282200 Rh6BG282700
rosa_wichuraiana	Rw6G024240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1244
AccB7I	CCANNNNNTGG	1 cut(s)	698
AccI	GTMKAC	1 cut(s)	2161
AccII	CGCG	7 cut(s)	90, 253, 302, 320, 383, 1813, 2505
AciI	CCGC	9 cut(s)	90, 300, 302, 305, 318, 456, 1102, 1534, 1928
AcoI	YGGCCR	1 cut(s)	19
AcsI	RAATTY	1 cut(s)	635
AcuI	CTGAAG	3 cut(s)	699, 1716, 2092
AcyI	GRCGYC	1 cut(s)	81
AdeI	CACNNNGTG	1 cut(s)	551
AfaI	GTAC	6 cut(s)	795, 946, 1249, 1507, 2502, 2553
AfiI	CCNNNNNNNGG	4 cut(s)	21, 28, 698, 1720
AhdI	GACNNNNNGTC	1 cut(s)	190
AjiI	CACGTC	1 cut(s)	1440
AjnI	CCWGG	4 cut(s)	50, 1107, 2343, 2538
AjuI	GAANNNNNNNTTGG	2 cut(s)	1184, 1216
Alw21I	GWGCWC	1 cut(s)	751
Alw26I	GTCTC	9 cut(s)	466, 533, 670, 1320, 1676, 1910, 2051, 2528, 2603
Alw44I	GTGCAC	1 cut(s)	747
AlwNI	CAGNNNCTG	1 cut(s)	2267
AoxI	GGCC	7 cut(s)	19, 43, 242, 275, 297, 997, 2206
ApaLI	GTGCAC	1 cut(s)	747
ApeKI	GCWGC	9 cut(s)	560, 563, 776, 1295, 1861, 2039, 2258, 2405, 2459
ApoI	RAATTY	1 cut(s)	635
ArsI	GACNNNNNNTTYG	2 cut(s)	648, 680
AspLEI	GCGC	3 cut(s)	310, 337, 385
AspS9I	GGNCC	4 cut(s)	12, 243, 1568, 2206
AsuC2I	CCSGG	1 cut(s)	1555
AsuHPI	GGTGA	4 cut(s)	612, 860, 1670, 2595
AsuNHI	GCTAGC	1 cut(s)	2078
AvaII	GGWCC	2 cut(s)	12, 1568
BaeGI	GKGCMC	1 cut(s)	751
BamHI	GGATCC	2 cut(s)	1204, 2369
BanII	GRGCYC	1 cut(s)	222
BarI	GAAGNNNNNNTAC	2 cut(s)	1054, 1086
BbsI	GAAGAC	4 cut(s)	1472, 1704, 1905, 2058
Bbv12I	GWGCWC	1 cut(s)	751
BbvCI	CCTCAGC	2 cut(s)	110, 465
BbvI	GCAGC	9 cut(s)	547, 550, 763, 1282, 1873, 2026, 2245, 2417, 2446
BceAI	ACGGC	6 cut(s)	34, 68, 262, 274, 491, 2267
BcgI	CGANNNNNNTGC	2 cut(s)	2495, 2529
BciT130I	CCWGG	4 cut(s)	52, 1109, 2345, 2540
BciVI	GTATCC	3 cut(s)	1268, 1426, 2643
BclI	TGATCA	1 cut(s)	2173
BcnI	CCSGG	1 cut(s)	1555
BcoDI	GTCTC	9 cut(s)	466, 533, 670, 1320, 1676, 1910, 2051, 2528, 2603
BfaI	CTAG	7 cut(s)	386, 549, 1184, 1500, 2079, 2183, 2549
BfmI	CTRYAG	1 cut(s)	2259
BfoI	RGCGCY	1 cut(s)	311
BfuI	GTATCC	3 cut(s)	1268, 1426, 2643
BglI	GCCNNNNNGGC	1 cut(s)	305
BlpI	GCTNAGC	1 cut(s)	772
Bme1390I	CCNGG	5 cut(s)	52, 1109, 1555, 2345, 2540
Bme18I	GGWCC	2 cut(s)	12, 1568
BmeRI	GACNNNNNGTC	1 cut(s)	190
BmgBI	CACGTC	1 cut(s)	1440
BmgT120I	GGNCC	4 cut(s)	12, 243, 1568, 2206
BmiI	GGNNCC	8 cut(s)	219, 1106, 1206, 1552, 1570, 1654, 1951, 2371
BmrFI	CCNGG	5 cut(s)	52, 1109, 1555, 2345, 2540
BmrI	ACTGGG	2 cut(s)	240, 1566
BmsI	GCATC	4 cut(s)	994, 1552, 1654, 2497
BmtI	GCTAGC	1 cut(s)	2082
BmuI	ACTGGG	2 cut(s)	240, 1566
BpiI	GAAGAC	4 cut(s)	1472, 1704, 1905, 2058
BplI	GAGNNNNNCTC	2 cut(s)	1343, 1375
BpmI	CTGGAG	2 cut(s)	751, 2075
Bpu10I	CCTNAGC	2 cut(s)	110, 465
Bpu1102I	GCTNAGC	1 cut(s)	772
BpuEI	CTTGAG	1 cut(s)	1177
BpuMI	CCSGG	1 cut(s)	1555
BsaHI	GRCGYC	1 cut(s)	81
BsaI	GGTCTC	3 cut(s)	533, 1676, 2051
BsaJI	CCNNGG	3 cut(s)	50, 300, 1209
BsaWI	WCCGGW	2 cut(s)	1655, 2605
BsaXI	ACNNNNNCTCC	4 cut(s)	24, 54, 2048, 2078
Bsc4I	CCNNNNNNNGG	4 cut(s)	21, 28, 698, 1720
Bse118I	RCCGGY	2 cut(s)	21, 409
Bse1I	ACTGG	7 cut(s)	246, 946, 1419, 1572, 1953, 2058, 2148
Bse3DI	GCAATG	3 cut(s)	716, 1384, 2511
BseBI	CCWGG	4 cut(s)	52, 1109, 2345, 2540
BseDI	CCNNGG	3 cut(s)	50, 300, 1209
BseGI	GGATG	4 cut(s)	501, 607, 1669, 1919
BseLI	CCNNNNNNNGG	4 cut(s)	21, 28, 698, 1720
BseMI	GCAATG	3 cut(s)	716, 1384, 2511
BseNI	ACTGG	7 cut(s)	246, 946, 1419, 1572, 1953, 2058, 2148
BseRI	GAGGAG	4 cut(s)	48, 51, 286, 1634
BseSI	GKGCMC	1 cut(s)	751
BseX3I	CGGCCG	1 cut(s)	19
BseXI	GCAGC	9 cut(s)	547, 550, 763, 1282, 1873, 2026, 2245, 2417, 2446
BseYI	CCCAGC	1 cut(s)	959
Bsh1236I	CGCG	7 cut(s)	90, 253, 302, 320, 383, 1813, 2505
Bsh1285I	CGRYCG	2 cut(s)	22, 318
BshFI	GGCC	7 cut(s)	21, 45, 244, 277, 299, 999, 2208
BsiEI	CGRYCG	2 cut(s)	22, 318
BsiHKAI	GWGCWC	1 cut(s)	751
BsiSI	CCGG	6 cut(s)	22, 410, 863, 1555, 1656, 2606
BslFI	GGGAC	2 cut(s)	1554, 1790
BslI	CCNNNNNNNGG	4 cut(s)	21, 28, 698, 1720
BsmAI	GTCTC	9 cut(s)	466, 533, 670, 1320, 1676, 1910, 2051, 2528, 2603
BsmBI	CGTCTC	1 cut(s)	466
BsmFI	GGGAC	2 cut(s)	1554, 1790
BsmI	GAATGC	1 cut(s)	2474
BsnI	GGCC	7 cut(s)	21, 45, 244, 277, 299, 999, 2208
Bso31I	GGTCTC	3 cut(s)	533, 1676, 2051
Bsp1286I	GDGCHC	2 cut(s)	222, 751
Bsp1720I	GCTNAGC	1 cut(s)	772
Bsp19I	CCATGG	1 cut(s)	1209
Bsp68I	TCGCGA	1 cut(s)	253
BspACI	CCGC	9 cut(s)	90, 300, 302, 305, 318, 456, 1102, 1534, 1928
BspANI	GGCC	7 cut(s)	21, 45, 244, 277, 299, 999, 2208
BspFNI	CGCG	7 cut(s)	90, 253, 302, 320, 383, 1813, 2505
BspLI	GGNNCC	8 cut(s)	219, 1106, 1206, 1552, 1570, 1654, 1951, 2371
BspMAI	CTGCAG	1 cut(s)	2263
BspOI	GCTAGC	1 cut(s)	2082
BspTNI	GGTCTC	3 cut(s)	533, 1676, 2051
BsrDI	GCAATG	3 cut(s)	716, 1384, 2511
BsrFI	RCCGGY	2 cut(s)	21, 409
BsrI	ACTGG	7 cut(s)	246, 946, 1419, 1572, 1953, 2058, 2148
BssAI	RCCGGY	2 cut(s)	21, 409
BssECI	CCNNGG	3 cut(s)	50, 300, 1209
BssNI	GRCGYC	1 cut(s)	81
BssT1I	CCWWGG	1 cut(s)	1209
Bst2UI	CCWGG	4 cut(s)	52, 1109, 2345, 2540
Bst4CI	ACNGT	3 cut(s)	949, 1703, 2269
Bst6I	CTCTTC	1 cut(s)	2292
BstACI	GRCGYC	1 cut(s)	81
BstDSI	CCRYGG	2 cut(s)	300, 1209
BstENI	CCTNNNNNAGG	1 cut(s)	1718
BstF5I	GGATG	4 cut(s)	501, 607, 1669, 1919
BstFNI	CGCG	7 cut(s)	90, 253, 302, 320, 383, 1813, 2505
BstH2I	RGCGCY	1 cut(s)	311
BstHHI	GCGC	3 cut(s)	310, 337, 385
BstMAI	GTCTC	9 cut(s)	466, 533, 670, 1320, 1676, 1910, 2051, 2528, 2603
BstMCI	CGRYCG	2 cut(s)	22, 318
BstMWI	GCNNNNNNNGC	8 cut(s)	202, 250, 296, 305, 311, 475, 1542, 2459
BstNI	CCWGG	4 cut(s)	52, 1109, 2345, 2540
BstNSI	RCATGY	2 cut(s)	365, 862
BstSCI	CCNGG	5 cut(s)	50, 1107, 1553, 2343, 2538
BstSFI	CTRYAG	1 cut(s)	2259
BstSLI	GKGCMC	1 cut(s)	751
BstUI	CGCG	7 cut(s)	90, 253, 302, 320, 383, 1813, 2505
BstV1I	GCAGC	9 cut(s)	547, 550, 763, 1282, 1873, 2026, 2245, 2417, 2446
BstV2I	GAAGAC	4 cut(s)	1472, 1704, 1905, 2058
BstX2I	RGATCY	7 cut(s)	1204, 1580, 1630, 1682, 1732, 2314, 2369
BstYI	RGATCY	7 cut(s)	1204, 1580, 1630, 1682, 1732, 2314, 2369
BstZI	CGGCCG	1 cut(s)	19
BsuI	GTATCC	3 cut(s)	1268, 1426, 2643
BsuRI	GGCC	7 cut(s)	21, 45, 244, 277, 299, 999, 2208
BtgI	CCRYGG	2 cut(s)	300, 1209
BtgZI	GCGATG	1 cut(s)	2520
BtrI	CACGTC	1 cut(s)	1440
BtsCI	GGATG	4 cut(s)	501, 607, 1669, 1919
BtsI	GCAGTG	1 cut(s)	203
BtsIMutI	CAGTG	5 cut(s)	203, 675, 954, 1778, 2265
BtuMI	TCGCGA	1 cut(s)	253
CaiI	CAGNNNCTG	1 cut(s)	2267
CfoI	GCGC	3 cut(s)	310, 337, 385
Cfr10I	RCCGGY	2 cut(s)	21, 409
Cfr13I	GGNCC	4 cut(s)	12, 243, 1568, 2206
Cfr42I	CCGCGG	1 cut(s)	303
CseI	GACGC	2 cut(s)	89, 524
Csp6I	GTAC	6 cut(s)	794, 945, 1248, 1506, 2501, 2552
CviQI	GTAC	6 cut(s)	794, 945, 1248, 1506, 2501, 2552
DraI	TTTAAA	1 cut(s)	973
DraIII	CACNNNGTG	1 cut(s)	551
DriI	GACNNNNNGTC	1 cut(s)	190
EaeI	YGGCCR	1 cut(s)	19
EagI	CGGCCG	1 cut(s)	19
Eam1104I	CTCTTC	1 cut(s)	2292
Eam1105I	GACNNNNNGTC	1 cut(s)	190
EarI	CTCTTC	1 cut(s)	2292
EclXI	CGGCCG	1 cut(s)	19
Eco130I	CCWWGG	1 cut(s)	1209
Eco147I	AGGCCT	2 cut(s)	45, 999
Eco24I	GRGCYC	1 cut(s)	222
Eco31I	GGTCTC	3 cut(s)	533, 1676, 2051
Eco47I	GGWCC	2 cut(s)	12, 1568
Eco52I	CGGCCG	1 cut(s)	19
Eco57I	CTGAAG	3 cut(s)	699, 1716, 2092
EcoNI	CCTNNNNNAGG	1 cut(s)	1718
EcoO109I	RGGNCCY	1 cut(s)	12
EcoRII	CCWGG	4 cut(s)	50, 1107, 2343, 2538
EcoT14I	CCWWGG	1 cut(s)	1209
EcoT38I	GRGCYC	1 cut(s)	222
ErhI	CCWWGG	1 cut(s)	1209
Esp3I	CGTCTC	1 cut(s)	466
FalI	AAGNNNNNCTT	2 cut(s)	1698, 1730
FaqI	GGGAC	2 cut(s)	1554, 1790
FauI	CCCGC	2 cut(s)	1541, 1921
FbaI	TGATCA	1 cut(s)	2173
FblI	GTMKAC	1 cut(s)	2161
FokI	GGATG	4 cut(s)	508, 614, 1676, 1906
FriOI	GRGCYC	1 cut(s)	222
FspBI	CTAG	7 cut(s)	386, 549, 1184, 1500, 2079, 2183, 2549
GlaI	GCGC	3 cut(s)	309, 336, 384
GsaI	CCCAGC	1 cut(s)	963
GsuI	CTGGAG	2 cut(s)	751, 2075
HaeII	RGCGCY	1 cut(s)	311
HaeIII	GGCC	7 cut(s)	21, 45, 244, 277, 299, 999, 2208
HapII	CCGG	6 cut(s)	22, 410, 863, 1555, 1656, 2606
HgaI	GACGC	2 cut(s)	89, 524
HhaI	GCGC	3 cut(s)	310, 337, 385
Hin1I	GRCGYC	1 cut(s)	81
Hin6I	GCGC	3 cut(s)	308, 335, 383
HinP1I	GCGC	3 cut(s)	308, 335, 383
HincII	GTYRAC	1 cut(s)	1711
HindII	GTYRAC	1 cut(s)	1711
HindIII	AAGCTT	3 cut(s)	707, 906, 1827
HpaII	CCGG	6 cut(s)	22, 410, 863, 1555, 1656, 2606
HphI	GGTGA	4 cut(s)	612, 860, 1670, 2595
Hpy166II	GTNNAC	7 cut(s)	367, 749, 1392, 1711, 2162, 2218, 2272
Hpy188III	TCNNGA	8 cut(s)	230, 252, 599, 929, 1156, 1485, 1730, 2177
Hpy8I	GTNNAC	7 cut(s)	367, 749, 1392, 1711, 2162, 2218, 2272
HpyCH4III	ACNGT	3 cut(s)	949, 1703, 2269
HpyCH4IV	ACGT	3 cut(s)	264, 323, 1439
HpyCH4V	TGCA	8 cut(s)	566, 654, 749, 816, 1640, 1742, 1861, 2261
HpyF10VI	GCNNNNNNNGC	8 cut(s)	202, 250, 296, 305, 311, 475, 1542, 2459
HpySE526I	ACGT	3 cut(s)	264, 323, 1439
Hsp92I	GRCGYC	1 cut(s)	81
HspAI	GCGC	3 cut(s)	308, 335, 383
KroI	GCCGGC	1 cut(s)	21
KroNI	GCCGGC	1 cut(s)	23
Ksp22I	TGATCA	1 cut(s)	2173
KspI	CCGCGG	1 cut(s)	303
LmnI	GCTCC	4 cut(s)	217, 358, 1104, 1364
Lsp1109I	GCAGC	9 cut(s)	547, 550, 763, 1282, 1873, 2026, 2245, 2417, 2446
LweI	GCATC	4 cut(s)	994, 1552, 1654, 2497
MaeI	CTAG	7 cut(s)	386, 549, 1184, 1500, 2079, 2183, 2549
MaeII	ACGT	3 cut(s)	264, 323, 1439
MaeIII	GTNAC	5 cut(s)	808, 2263, 2306, 2412, 2534
MfeI	CAATTG	2 cut(s)	660, 1856
MflI	RGATCY	7 cut(s)	1204, 1580, 1630, 1682, 1732, 2314, 2369
MhlI	GDGCHC	2 cut(s)	222, 751
MlyI	GAGTC	6 cut(s)	59, 179, 481, 758, 1735, 2605
MmeI	TCCRAC	3 cut(s)	87, 1182, 2541
MroNI	GCCGGC	1 cut(s)	21
MseI	TTAA	6 cut(s)	633, 869, 972, 1520, 1986, 2591
MslI	CAYNNNNRTG	2 cut(s)	1442, 1521
MspA1I	CMGCKG	4 cut(s)	302, 776, 1864, 2408
MspI	CCGG	6 cut(s)	22, 410, 863, 1555, 1656, 2606
MspR9I	CCNGG	5 cut(s)	52, 1109, 1555, 2345, 2540
MunI	CAATTG	2 cut(s)	660, 1856
Mva1269I	GAATGC	1 cut(s)	2474
MvaI	CCWGG	4 cut(s)	52, 1109, 2345, 2540
MvnI	CGCG	7 cut(s)	90, 253, 302, 320, 383, 1813, 2505
MwoI	GCNNNNNNNGC	8 cut(s)	202, 250, 296, 305, 311, 475, 1542, 2459
NaeI	GCCGGC	1 cut(s)	23
NciI	CCSGG	1 cut(s)	1555
NcoI	CCATGG	1 cut(s)	1209
NgoMIV	GCCGGC	1 cut(s)	21
NheI	GCTAGC	1 cut(s)	2078
NlaIV	GGNNCC	8 cut(s)	219, 1106, 1206, 1552, 1570, 1654, 1951, 2371
NmuCI	GTSAC	2 cut(s)	2263, 2306
NruI	TCGCGA	1 cut(s)	253
NspI	RCATGY	2 cut(s)	365, 862
PaeI	GCATGC	1 cut(s)	862
PceI	AGGCCT	2 cut(s)	45, 999
PcsI	WCGNNNNNNNCGW	3 cut(s)	102, 185, 287
PctI	GAATGC	1 cut(s)	2474
PdiI	GCCGGC	1 cut(s)	23
PfeI	GAWTC	7 cut(s)	129, 212, 617, 802, 1883, 2014, 2639
PflFI	GACNNNGTC	2 cut(s)	371, 2062
PflMI	CCANNNNNTGG	1 cut(s)	698
PfoI	TCCNGGA	1 cut(s)	1553
PleI	GAGTC	6 cut(s)	59, 179, 481, 758, 1734, 2605
PpsI	GAGTC	6 cut(s)	59, 179, 481, 758, 1734, 2605
PpuMI	RGGWCCY	1 cut(s)	12
PsiI	TTATAA	1 cut(s)	1244
Psp5II	RGGWCCY	1 cut(s)	12
Psp6I	CCWGG	4 cut(s)	50, 1107, 2343, 2538
PspFI	CCCAGC	1 cut(s)	959
PspGI	CCWGG	4 cut(s)	50, 1107, 2343, 2538
PspN4I	GGNNCC	8 cut(s)	219, 1106, 1206, 1552, 1570, 1654, 1951, 2371
PspPI	GGNCC	4 cut(s)	12, 243, 1568, 2206
PspPPI	RGGWCCY	1 cut(s)	12
PsrI	GAACNNNNNNTAC	2 cut(s)	1933, 1965
PstI	CTGCAG	1 cut(s)	2263
PstNI	CAGNNNCTG	1 cut(s)	2267
PsuI	RGATCY	7 cut(s)	1204, 1580, 1630, 1682, 1732, 2314, 2369
PsyI	GACNNNGTC	2 cut(s)	371, 2062
PvuII	CAGCTG	3 cut(s)	776, 1864, 2408
RruI	TCGCGA	1 cut(s)	253
RsaI	GTAC	6 cut(s)	795, 946, 1249, 1507, 2502, 2553
RsaNI	GTAC	6 cut(s)	794, 945, 1248, 1506, 2501, 2552
RseI	CAYNNNNRTG	2 cut(s)	1442, 1521
SacII	CCGCGG	1 cut(s)	303
SaqAI	TTAA	6 cut(s)	633, 869, 972, 1520, 1986, 2591
Sau96I	GGNCC	4 cut(s)	12, 243, 1568, 2206
SchI	GAGTC	6 cut(s)	59, 179, 481, 758, 1735, 2605
ScrFI	CCNGG	5 cut(s)	52, 1109, 1555, 2345, 2540
SduI	GDGCHC	2 cut(s)	222, 751
SfaNI	GCATC	4 cut(s)	994, 1552, 1654, 2497
SfcI	CTRYAG	1 cut(s)	2259
Sfr303I	CCGCGG	1 cut(s)	303
SgrBI	CCGCGG	1 cut(s)	303
SinI	GGWCC	2 cut(s)	12, 1568
SmiMI	CAYNNNNRTG	2 cut(s)	1442, 1521
SmlI	CTYRAG	1 cut(s)	1156
SmoI	CTYRAG	1 cut(s)	1156
SphI	GCATGC	1 cut(s)	862
SseBI	AGGCCT	2 cut(s)	45, 999
SsiI	CCGC	9 cut(s)	90, 300, 302, 305, 318, 456, 1102, 1534, 1928
SspMI	CTAG	7 cut(s)	386, 549, 1184, 1500, 2079, 2183, 2549
StuI	AGGCCT	2 cut(s)	45, 999
StyD4I	CCNGG	5 cut(s)	50, 1107, 1553, 2343, 2538
StyI	CCWWGG	1 cut(s)	1209
TaaI	ACNGT	3 cut(s)	949, 1703, 2269
TaiI	ACGT	3 cut(s)	267, 326, 1442
TaqI	TCGA	3 cut(s)	210, 281, 1807
TatI	WGTACW	1 cut(s)	793
TauI	GCSGC	5 cut(s)	93, 302, 305, 308, 459
TfiI	GAWTC	7 cut(s)	129, 212, 617, 802, 1883, 2014, 2639
Tru1I	TTAA	6 cut(s)	633, 869, 972, 1520, 1986, 2591
Tru9I	TTAA	6 cut(s)	633, 869, 972, 1520, 1986, 2591
TscAI	CASTG	5 cut(s)	203, 682, 954, 1778, 2272
TseFI	GTSAC	2 cut(s)	2263, 2306
TseI	GCWGC	9 cut(s)	560, 563, 776, 1295, 1861, 2039, 2258, 2405, 2459
Tsp45I	GTSAC	2 cut(s)	2263, 2306
TspDTI	ATGAA	6 cut(s)	839, 1205, 1353, 1764, 2012, 2082
TspRI	CASTG	5 cut(s)	203, 682, 954, 1778, 2272
Tth111I	GACNNNGTC	2 cut(s)	371, 2062
Van91I	CCANNNNNTGG	1 cut(s)	698
VneI	GTGCAC	1 cut(s)	747
VpaK11BI	GGWCC	2 cut(s)	12, 1568
XagI	CCTNNNNNAGG	1 cut(s)	1718
XapI	RAATTY	1 cut(s)	635
XceI	RCATGY	2 cut(s)	365, 862
XmiI	GTMKAC	1 cut(s)	2161
XspI	CTAG	7 cut(s)	386, 549, 1184, 1500, 2079, 2183, 2549

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.