Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012578

Physical Location & Seq

Genomic Coordinates Reverse (-)

28926193 .. 28928772

Gene Structure 2580 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1193100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 873 bp

ATGGCGTTGGAGCGAGCAACTCCGGCGGCAACAAGCGCAACCTTCCTCGCAAACCCCCAAGTTCCCGCCTTTTTCCCATCCACCAACCACTTGGCCTTCGCACGGCGCCGTTTCAATCTCAAATGCTCGATGGAGTACAGTAGAGACCAGTACAACGGCAACGGGAGCAGCATTCAGGTGCCGATAGCGTACCCTAGACCGGTGGAGGTTCAGTGGAAGAAGGAGCTGTGCAATACGGTGCACCTCATCGGCGTCGTCGGTCTTCCCGTCGAAATTAAGCACCTCCCCTCCGGAAAGGTCCTCGCTTGGACCCGCCTCGCCGTTAAGAAGTCCGCCCCCGACACCTCTTGGATTAATCTGACATTTTGGGATGAGCTAGCACATGTTGCTTTTCAGCATGTAGAGAAAGGCCAACAAATATATGTAACGGGCCGTCTGATATCAGATTCTGTTGAAACCGATGAAGGGAAGCAGCAGACCTACTATAAGGTAGTTGTTCAGCAACTGAATTTTGTTGAAAAGGGCTTTTCATCGCCGGCTTTGAAAGATCACGACTCTGATTCATTTACAGCAGGTAGAAACCTTGGCAACAATGCCACAAAGAACATAACGGAAACCACACAAGAATTATGGCAAGCCTTCTTTGCTAATCCAGTTGAATGGTGGGATAATAGGAAGACCAAGAGGAACCCAAAGTATCCGGATTTTAAGCATAAAGATACTGGAGAAGCCTTGTGGATTGAAGGCCGGAACAATCCGCAGTGGGTGAAGTCCCAACTGGCTATACTGGATACAAGAATGGGAACCCTTGATGACCAAGATCATAACATTCATACAAATATATTTGCCGGTGATAGTTTTTCGCCTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000002 GO:0000018 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006996 GO:0007005 GO:0008150 GO:0009892 GO:0009987 GO:0010605 GO:0016043 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045910 GO:0045934 GO:0048046 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0060255 GO:0065007 GO:0071840 GO:0080090 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SSB

Protein Analysis

291

Amino Acids

32.94

Weight (kDa)

7.8

Isoelectric Point (pI)

40.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SSB	PF00436	78 - 171	7.9e-17	Single-strand binding protein family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012578)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g22540 FvH4_2g22540
malus_domestica	MD05G1206000.v1.1 MD10G1193100.v1.1
prunus_persica	Prupe.8G232500_v2.0.a1
pyrus_communis	pycom05g19220 pycom10g16660
rosa_chinensis	RchiOBHm_Chr6g0289591
rosa_laevigata	RLG00000012290
rosa_multiflora	Rmu_sc0001527.1_g000063 Rmu_sc0010363.1_g000003
rosa_roxburghii	Rroxscaffold_7G00177830
rosa_rugosa	Rorug06G0204900 Rorug06G0205000
rosa_samantha	Rh6AG316500 Rh6BG322900 Rh6CG329400 Rh6DG314900
rosa_wichuraiana	Rw6G027290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	563
AccB1I	GGYRCC	2 cut(s)	105, 178
AccIII	TCCGGA	2 cut(s)	290, 700
AciI	CCGC	5 cut(s)	26, 66, 313, 333, 758
AcsI	RAATTY	1 cut(s)	508
AcyI	GRCGYC	2 cut(s)	106, 252
AfaI	GTAC	3 cut(s)	137, 152, 191
AfiI	CCNNNNNNNGG	3 cut(s)	102, 199, 465
AflIII	ACRYGT	1 cut(s)	382
AgeI	ACCGGT	1 cut(s)	199
AgsI	TTSAA	6 cut(s)	115, 455, 518, 544, 659, 743
AluBI	AGCT	2 cut(s)	226, 376
AluI	AGCT	2 cut(s)	226, 376
Alw21I	GWGCWC	1 cut(s)	243
Alw26I	GTCTC	1 cut(s)	138
Alw44I	GTGCAC	1 cut(s)	239
AlwNI	CAGNNNCTG	2 cut(s)	449, 505
Aor13HI	TCCGGA	2 cut(s)	290, 700
AoxI	GGCC	4 cut(s)	93, 409, 430, 745
ApaLI	GTGCAC	1 cut(s)	239
ApeKI	GCWGC	2 cut(s)	168, 472
ApoI	RAATTY	1 cut(s)	508
AseI	ATTAAT	1 cut(s)	354
AsiGI	ACCGGT	1 cut(s)	199
AspLEI	GCGC	2 cut(s)	38, 108
AspS9I	GGNCC	3 cut(s)	298, 309, 430
AsuHPI	GGTGA	2 cut(s)	778, 863
AsuNHI	GCTAGC	1 cut(s)	376
AvaII	GGWCC	2 cut(s)	298, 309
BaeGI	GKGCMC	1 cut(s)	243
BanI	GGYRCC	2 cut(s)	105, 178
BbsI	GAAGAC	2 cut(s)	254, 683
Bbv12I	GWGCWC	1 cut(s)	243
BbvI	GCAGC	2 cut(s)	180, 484
BccI	CCATC	2 cut(s)	85, 124
BceAI	ACGGC	5 cut(s)	93, 119, 172, 305, 417
BciVI	GTATCC	2 cut(s)	708, 784
BcoDI	GTCTC	1 cut(s)	138
BfaI	CTAG	2 cut(s)	195, 377
BfoI	RGCGCY	1 cut(s)	109
BfuAI	ACCTGC	1 cut(s)	563
BfuI	GTATCC	2 cut(s)	708, 784
BisI	GCNGC	3 cut(s)	27, 169, 473
BlsI	GCNGC	3 cut(s)	28, 170, 474
Bme18I	GGWCC	2 cut(s)	298, 309
BmgT120I	GGNCC	3 cut(s)	298, 309, 430
BmiI	GGNNCC	5 cut(s)	107, 180, 311, 689, 805
BmtI	GCTAGC	1 cut(s)	380
BpiI	GAAGAC	2 cut(s)	254, 683
BpmI	CTGGAG	1 cut(s)	744
BsaHI	GRCGYC	2 cut(s)	106, 252
BsaI	GGTCTC	1 cut(s)	138
BsaJI	CCNNGG	1 cut(s)	583
BsaWI	WCCGGW	3 cut(s)	199, 290, 700
BsaXI	ACNNNNNCTCC	4 cut(s)	197, 227, 272, 302
Bsc4I	CCNNNNNNNGG	3 cut(s)	102, 199, 465
Bse118I	RCCGGY	3 cut(s)	199, 535, 848
Bse1I	ACTGG	5 cut(s)	148, 653, 727, 783, 792
BseAI	TCCGGA	2 cut(s)	290, 700
BseDI	CCNNGG	1 cut(s)	583
BseGI	GGATG	2 cut(s)	77, 376
BseLI	CCNNNNNNNGG	3 cut(s)	102, 199, 465
BseNI	ACTGG	5 cut(s)	148, 653, 727, 783, 792
BseSI	GKGCMC	1 cut(s)	243
BseXI	GCAGC	2 cut(s)	180, 484
BshFI	GGCC	4 cut(s)	95, 411, 432, 747
BshNI	GGYRCC	2 cut(s)	105, 178
BshTI	ACCGGT	1 cut(s)	199
BsiHKAI	GWGCWC	1 cut(s)	243
BsiSI	CCGG	7 cut(s)	23, 200, 291, 536, 701, 748, 849
BslFI	GGGAC	1 cut(s)	757
BslI	CCNNNNNNNGG	3 cut(s)	102, 199, 465
BsmAI	GTCTC	1 cut(s)	138
BsmFI	GGGAC	1 cut(s)	757
BsmI	GAATGC	1 cut(s)	171
BsnI	GGCC	4 cut(s)	95, 411, 432, 747
Bso31I	GGTCTC	1 cut(s)	138
Bsp1286I	GDGCHC	1 cut(s)	243
Bsp13I	TCCGGA	2 cut(s)	290, 700
Bsp143I	GATC	2 cut(s)	547, 820
BspACI	CCGC	5 cut(s)	26, 66, 313, 333, 758
BspANI	GGCC	4 cut(s)	95, 411, 432, 747
BspEI	TCCGGA	2 cut(s)	290, 700
BspLI	GGNNCC	5 cut(s)	107, 180, 311, 689, 805
BspMI	ACCTGC	1 cut(s)	563
BspOI	GCTAGC	1 cut(s)	380
BspT107I	GGYRCC	2 cut(s)	105, 178
BspTNI	GGTCTC	1 cut(s)	138
BsrFI	RCCGGY	3 cut(s)	199, 535, 848
BsrI	ACTGG	5 cut(s)	148, 653, 727, 783, 792
BssAI	RCCGGY	3 cut(s)	199, 535, 848
BssECI	CCNNGG	1 cut(s)	583
BssMI	GATC	2 cut(s)	547, 820
BssNI	GRCGYC	2 cut(s)	106, 252
BssT1I	CCWWGG	1 cut(s)	583
Bst4CI	ACNGT	2 cut(s)	140, 238
BstACI	GRCGYC	2 cut(s)	106, 252
BstAPI	GCANNNNNTGC	1 cut(s)	386
BstC8I	GCNNGC	4 cut(s)	15, 378, 537, 636
BstF5I	GGATG	2 cut(s)	77, 376
BstH2I	RGCGCY	1 cut(s)	109
BstHHI	GCGC	2 cut(s)	38, 108
BstKTI	GATC	2 cut(s)	550, 823
BstMAI	GTCTC	1 cut(s)	138
BstMBI	GATC	2 cut(s)	547, 820
BstMWI	GCNNNNNNNGC	5 cut(s)	23, 35, 165, 386, 644
BstNSI	RCATGY	2 cut(s)	386, 401
BstSLI	GKGCMC	1 cut(s)	243
BstV1I	GCAGC	2 cut(s)	180, 484
BstV2I	GAAGAC	2 cut(s)	254, 683
BstXI	CCANNNNNNTGG	2 cut(s)	91, 660
BsuI	GTATCC	2 cut(s)	708, 784
BsuRI	GGCC	4 cut(s)	95, 411, 432, 747
BtgZI	GCGATG	1 cut(s)	516
BtsCI	GGATG	2 cut(s)	77, 376
BtsI	GCAGTG	1 cut(s)	767
BtsIMutI	CAGTG	2 cut(s)	218, 767
BveI	ACCTGC	1 cut(s)	563
Cac8I	GCNNGC	4 cut(s)	15, 378, 537, 636
CaiI	CAGNNNCTG	2 cut(s)	449, 505
CfoI	GCGC	2 cut(s)	38, 108
Cfr10I	RCCGGY	3 cut(s)	199, 535, 848
Cfr13I	GGNCC	3 cut(s)	298, 309, 430
CseI	GACGC	1 cut(s)	241
Csp6I	GTAC	3 cut(s)	136, 151, 190
CspAI	ACCGGT	1 cut(s)	199
CviAII	CATG	2 cut(s)	383, 398
CviQI	GTAC	3 cut(s)	136, 151, 190
DinI	GGCGCC	1 cut(s)	107
DpnI	GATC	2 cut(s)	549, 822
DpnII	GATC	2 cut(s)	547, 820
EciI	GGCGGA	1 cut(s)	322
Eco130I	CCWWGG	1 cut(s)	583
Eco31I	GGTCTC	1 cut(s)	138
Eco32I	GATATC	1 cut(s)	441
Eco47I	GGWCC	2 cut(s)	298, 309
EcoO109I	RGGNCCY	1 cut(s)	298
EcoRV	GATATC	1 cut(s)	441
EcoT14I	CCWWGG	1 cut(s)	583
EgeI	GGCGCC	1 cut(s)	107
EheI	GGCGCC	1 cut(s)	107
ErhI	CCWWGG	1 cut(s)	583
FaeI	CATG	2 cut(s)	386, 401
FaqI	GGGAC	1 cut(s)	757
FatI	CATG	2 cut(s)	382, 397
FauI	CCCGC	2 cut(s)	73, 320
Fnu4HI	GCNGC	3 cut(s)	27, 169, 473
FokI	GGATG	2 cut(s)	64, 383
Fsp4HI	GCNGC	3 cut(s)	27, 169, 473
FspBI	CTAG	2 cut(s)	195, 377
GlaI	GCGC	2 cut(s)	37, 107
GluI	GCNGC	3 cut(s)	27, 169, 473
GsuI	CTGGAG	1 cut(s)	744
HaeII	RGCGCY	1 cut(s)	109
HaeIII	GGCC	4 cut(s)	95, 411, 432, 747
HapII	CCGG	7 cut(s)	23, 200, 291, 536, 701, 748, 849
HgaI	GACGC	1 cut(s)	241
HhaI	GCGC	2 cut(s)	38, 108
Hin1I	GRCGYC	2 cut(s)	106, 252
Hin1II	CATG	2 cut(s)	386, 401
Hin6I	GCGC	2 cut(s)	36, 106
HinP1I	GCGC	2 cut(s)	36, 106
HinfI	GANTC	3 cut(s)	446, 554, 560
HpaII	CCGG	7 cut(s)	23, 200, 291, 536, 701, 748, 849
HphI	GGTGA	2 cut(s)	778, 863
Hpy166II	GTNNAC	1 cut(s)	241
Hpy188I	TCNGA	4 cut(s)	360, 438, 445, 559
Hpy188III	TCNNGA	3 cut(s)	291, 551, 701
Hpy8I	GTNNAC	1 cut(s)	241
Hpy99I	CGWCG	3 cut(s)	257, 260, 272
HpyAV	CCTTC	6 cut(s)	52, 106, 214, 458, 649, 737
HpyCH4III	ACNGT	2 cut(s)	140, 238
HpyCH4V	TGCA	2 cut(s)	231, 241
HpyF10VI	GCNNNNNNNGC	5 cut(s)	23, 35, 165, 386, 644
Hsp92I	GRCGYC	2 cut(s)	106, 252
Hsp92II	CATG	2 cut(s)	386, 401
HspAI	GCGC	2 cut(s)	36, 106
KasI	GGCGCC	1 cut(s)	105
Kpn2I	TCCGGA	2 cut(s)	290, 700
KroI	GCCGGC	1 cut(s)	535
KroNI	GCCGGC	1 cut(s)	537
Kzo9I	GATC	2 cut(s)	547, 820
LmnI	GCTCC	3 cut(s)	10, 165, 223
Lsp1109I	GCAGC	2 cut(s)	180, 484
MaeI	CTAG	2 cut(s)	195, 377
MaeIII	GTNAC	1 cut(s)	424
MalI	GATC	2 cut(s)	549, 822
MboI	GATC	2 cut(s)	547, 820
MboII	GAAGA	3 cut(s)	229, 254, 688
MhlI	GDGCHC	1 cut(s)	243
MluCI	AATT	3 cut(s)	273, 508, 626
Mly113I	GGCGCC	1 cut(s)	106
MlyI	GAGTC	1 cut(s)	548
MnlI	CCTC	9 cut(s)	56, 199, 254, 293, 298, 311, 326, 355, 678
MroI	TCCGGA	2 cut(s)	290, 700
MroNI	GCCGGC	1 cut(s)	535
MseI	TTAA	4 cut(s)	276, 324, 354, 708
MslI	CAYNNNNRTG	1 cut(s)	176
MspI	CCGG	7 cut(s)	23, 200, 291, 536, 701, 748, 849
Mva1269I	GAATGC	1 cut(s)	171
MwoI	GCNNNNNNNGC	5 cut(s)	23, 35, 165, 386, 644
NaeI	GCCGGC	1 cut(s)	537
NarI	GGCGCC	1 cut(s)	106
NdeII	GATC	2 cut(s)	547, 820
NgoMIV	GCCGGC	1 cut(s)	535
NheI	GCTAGC	1 cut(s)	376
NlaIII	CATG	2 cut(s)	386, 401
NlaIV	GGNNCC	5 cut(s)	107, 180, 311, 689, 805
NspI	RCATGY	2 cut(s)	386, 401
PciI	ACATGT	1 cut(s)	382
PcsI	WCGNNNNNNNCGW	1 cut(s)	264
PctI	GAATGC	1 cut(s)	171
PdiI	GCCGGC	1 cut(s)	537
PfeI	GAWTC	2 cut(s)	446, 560
PinAI	ACCGGT	1 cut(s)	199
PkrI	GCNGC	3 cut(s)	28, 170, 474
PleI	GAGTC	1 cut(s)	548
PluTI	GGCGCC	1 cut(s)	109
PpsI	GAGTC	1 cut(s)	548
PpuMI	RGGWCCY	1 cut(s)	298
PscI	ACATGT	1 cut(s)	382
PshBI	ATTAAT	1 cut(s)	354
Psp5II	RGGWCCY	1 cut(s)	298
PspN4I	GGNNCC	5 cut(s)	107, 180, 311, 689, 805
PspPI	GGNCC	3 cut(s)	298, 309, 430
PspPPI	RGGWCCY	1 cut(s)	298
PstNI	CAGNNNCTG	2 cut(s)	449, 505
RsaI	GTAC	3 cut(s)	137, 152, 191
RsaNI	GTAC	3 cut(s)	136, 151, 190
RseI	CAYNNNNRTG	1 cut(s)	176
SaqAI	TTAA	4 cut(s)	276, 324, 354, 708
SatI	GCNGC	3 cut(s)	27, 169, 473
Sau3AI	GATC	2 cut(s)	547, 820
Sau96I	GGNCC	3 cut(s)	298, 309, 430
SchI	GAGTC	1 cut(s)	548
SduI	GDGCHC	1 cut(s)	243
SfoI	GGCGCC	1 cut(s)	107
SinI	GGWCC	2 cut(s)	298, 309
SmiMI	CAYNNNNRTG	1 cut(s)	176
Sse9I	AATT	3 cut(s)	273, 508, 626
SsiI	CCGC	5 cut(s)	26, 66, 313, 333, 758
SspDI	GGCGCC	1 cut(s)	105
SspMI	CTAG	2 cut(s)	195, 377
StyI	CCWWGG	1 cut(s)	583
TaaI	ACNGT	2 cut(s)	140, 238
TaqI	TCGA	2 cut(s)	128, 270
TaqII	GACCGA	1 cut(s)	248
TasI	AATT	3 cut(s)	273, 508, 626
TatI	WGTACW	2 cut(s)	135, 150
TauI	GCSGC	1 cut(s)	29
TfiI	GAWTC	2 cut(s)	446, 560
Tru1I	TTAA	4 cut(s)	276, 324, 354, 708
Tru9I	TTAA	4 cut(s)	276, 324, 354, 708
TscAI	CASTG	2 cut(s)	218, 767
TseI	GCWGC	2 cut(s)	168, 472
TspDTI	ATGAA	4 cut(s)	477, 519, 552, 821
TspGWI	ACGGA	1 cut(s)	626
TspRI	CASTG	2 cut(s)	218, 767
VneI	GTGCAC	1 cut(s)	239
VpaK11BI	GGWCC	2 cut(s)	298, 309
VspI	ATTAAT	1 cut(s)	354
XapI	RAATTY	1 cut(s)	508
XceI	RCATGY	2 cut(s)	386, 401
XcmI	CCANNNNNNNNNTGG	2 cut(s)	88, 660
XspI	CTAG	2 cut(s)	195, 377

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.