RLG00000012290

Protein OSB2, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012578
Physical Location & Seq
Forward (+)
17199947 .. 17201792
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012290

Sequence Viewer

Length: 864 bp
ATGGCGTTAGACCGAGCACTACAAGCAGCAACCTCCACAAACTTCCTCACAAACCCCCAAATCCCCTCTATATCCCCAAACACAACCACCACCCACAACCACTTGGCCTTCGCGCGACGTCGTTTCAGCTTCAAATGCGCGGTGGAGTACAGCAGAGACCACTACAGCAGCAGCGTCCAAGTTCCGGTGGCGCATCCTAGACCGGCGGAGGTCCAGTGGAAGAAGGAGCTCTGTAATACGGTGCAACTCATCGGCGTCGTCGGTCTTCCCGTCGAAATTAAGCACCTTCCCTCCGGTAAAGTCCTCGCCTGGACCCGCCTCGCCGTCAAGAAATCCGCCACCGACACCTCATGGATCAATTTGACATTTTGGGATGAGATAGCGCATGTTGCTTTTCAGCATTTAGAGAAAGGCCATCAGATATATGTTTCTGGAAGACTGATTTCAGATGCTGTTGAAACCAATGAGGGGAAACAGCAGACCTATTACAAGGTTGTTGTTCAGCAACTGAATTTTGTTGAAAAGAGCTCCCCAGCGCCGAATGGTGGTCAGGACTCGGATTCCTTTACACCAGGCAAAAGTTCTAGCCACAGCTTTACGAAGCACACAGAAACCACACAAGAATTGTGGCAAGCATTCTTTGCTAATCCAGTAGAGTGGTGGGATAATAGGAAGACCAAGAGGAATTCAAAATATCCAGATTTTAAGCACAAAGACACCGGAGAAGCCTTGTGGATTGAAGGCCGGAACAATCCTCCATGGGTGAAGTCCCAACTTGCTATACTGGATACAAGAATGGGGACCGCTAATGATCAAGATCTTAAGACGCATTCAAACCTGTTTGCTGATGATAATTATTTCTAA

Protein Analysis

288

Amino Acids

32.57

Weight (kDa)

8.72

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 79 - 172 3.6e-16 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 121
AccII CGCG 3 cut(s) 113, 115, 140
AciI CCGC 5 cut(s) 140, 206, 316, 336, 804
AclWI GGATC 1 cut(s) 362
AcsI RAATTY 2 cut(s) 511, 685
AcyI GRCGYC 2 cut(s) 118, 255
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 3 cut(s) 184, 468, 545
AflII CTTAAG 1 cut(s) 821
AgsI TTSAA 6 cut(s) 133, 458, 521, 690, 740, 834
AjnI CCWGG 2 cut(s) 308, 571
AluBI AGCT 4 cut(s) 129, 229, 528, 594
AluI AGCT 4 cut(s) 129, 229, 528, 594
Alw21I GWGCWC 3 cut(s) 19, 231, 530
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 1 cut(s) 362
AlwNI CAGNNNCTG 2 cut(s) 452, 508
AoxI GGCC 3 cut(s) 105, 412, 742
ApeKI GCWGC 3 cut(s) 26, 168, 171
ApoI RAATTY 2 cut(s) 511, 685
AspLEI GCGC 5 cut(s) 115, 140, 193, 385, 538
AspS9I GGNCC 3 cut(s) 211, 312, 801
AsuHPI GGTGA 1 cut(s) 775
AvaII GGWCC 3 cut(s) 211, 312, 801
BanII GRGCYC 2 cut(s) 231, 530
BbsI GAAGAC 3 cut(s) 257, 442, 680
Bbv12I GWGCWC 3 cut(s) 19, 231, 530
BbvI GCAGC 3 cut(s) 38, 180, 183
BccI CCATC 1 cut(s) 423
BceAI ACGGC 1 cut(s) 308
BciT130I CCWGG 2 cut(s) 310, 573
BciVI GTATCC 1 cut(s) 781
BclI TGATCA 1 cut(s) 811
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 2 cut(s) 198, 585
BfmI CTRYAG 1 cut(s) 163
BfoI RGCGCY 1 cut(s) 539
BfrI CTTAAG 1 cut(s) 821
BfuI GTATCC 1 cut(s) 781
BglII AGATCT 1 cut(s) 817
BisI GCNGC 3 cut(s) 27, 169, 172
BlsI GCNGC 3 cut(s) 28, 170, 173
Bme1390I CCNGG 2 cut(s) 310, 573
Bme18I GGWCC 3 cut(s) 211, 312, 801
BmgT120I GGNCC 3 cut(s) 211, 312, 801
BmiI GGNNCC 2 cut(s) 314, 802
BmrFI CCNGG 2 cut(s) 310, 573
BmsI GCATC 2 cut(s) 202, 439
BpiI GAAGAC 3 cut(s) 257, 442, 680
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 2 cut(s) 118, 255
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 758
BsaWI WCCGGW 3 cut(s) 184, 293, 719
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 275, 305
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 468, 545
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 3 cut(s) 214, 650, 789
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 2 cut(s) 310, 573
BseDI CCNNGG 1 cut(s) 758
BseGI GGATG 2 cut(s) 193, 379
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 3 cut(s) 184, 468, 545
BseNI ACTGG 3 cut(s) 214, 650, 789
BseXI GCAGC 3 cut(s) 38, 180, 183
BseYI CCCAGC 1 cut(s) 532
Bsh1236I CGCG 3 cut(s) 113, 115, 140
BshFI GGCC 3 cut(s) 107, 414, 744
BsiHKAI GWGCWC 3 cut(s) 19, 231, 530
BsiSI CCGG 5 cut(s) 185, 203, 294, 720, 745
BslFI GGGAC 2 cut(s) 754, 814
BslI CCNNNNNNNGG 3 cut(s) 184, 468, 545
BsmAI GTCTC 1 cut(s) 150
BsmFI GGGAC 2 cut(s) 754, 814
BsmI GAATGC 2 cut(s) 635, 829
BsnI GGCC 3 cut(s) 107, 414, 744
Bso31I GGTCTC 1 cut(s) 150
Bsp1286I GDGCHC 3 cut(s) 19, 231, 530
Bsp143I GATC 3 cut(s) 354, 811, 817
Bsp19I CCATGG 1 cut(s) 758
BspACI CCGC 5 cut(s) 140, 206, 316, 336, 804
BspANI GGCC 3 cut(s) 107, 414, 744
BspFNI CGCG 3 cut(s) 113, 115, 140
BspLI GGNNCC 2 cut(s) 314, 802
BspPI GGATC 1 cut(s) 362
BspTI CTTAAG 1 cut(s) 821
BspTNI GGTCTC 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 3 cut(s) 214, 650, 789
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 1 cut(s) 758
BssMI GATC 3 cut(s) 354, 811, 817
BssNI GRCGYC 2 cut(s) 118, 255
BssT1I CCWWGG 1 cut(s) 758
Bst2UI CCWGG 2 cut(s) 310, 573
Bst4CI ACNGT 1 cut(s) 241
BstACI GRCGYC 2 cut(s) 118, 255
BstAFI CTTAAG 1 cut(s) 821
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 1 cut(s) 633
BstDSI CCRYGG 1 cut(s) 758
BstF5I GGATG 2 cut(s) 193, 379
BstFNI CGCG 3 cut(s) 113, 115, 140
BstH2I RGCGCY 1 cut(s) 539
BstHHI GCGC 5 cut(s) 115, 140, 193, 385, 538
BstKTI GATC 3 cut(s) 357, 814, 820
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 3 cut(s) 354, 811, 817
BstMWI GCNNNNNNNGC 4 cut(s) 23, 135, 389, 641
BstNI CCWGG 2 cut(s) 310, 573
BstNSI RCATGY 1 cut(s) 389
BstSCI CCNGG 2 cut(s) 308, 571
BstSFI CTRYAG 1 cut(s) 163
BstUI CGCG 3 cut(s) 113, 115, 140
BstV1I GCAGC 3 cut(s) 38, 180, 183
BstV2I GAAGAC 3 cut(s) 257, 442, 680
BstX2I RGATCY 1 cut(s) 817
BstXI CCANNNNNNTGG 1 cut(s) 657
BstYI RGATCY 1 cut(s) 817
BsuI GTATCC 1 cut(s) 781
BsuRI GGCC 3 cut(s) 107, 414, 744
BtgI CCRYGG 1 cut(s) 758
BtsCI GGATG 2 cut(s) 193, 379
BtsIMutI CAGTG 1 cut(s) 221
Cac8I GCNNGC 1 cut(s) 633
CaiI CAGNNNCTG 2 cut(s) 452, 508
CfoI GCGC 5 cut(s) 115, 140, 193, 385, 538
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 3 cut(s) 211, 312, 801
CseI GACGC 3 cut(s) 163, 244, 835
Csp6I GTAC 1 cut(s) 148
CspCI CAANNNNNGTGG 2 cut(s) 608, 643
CviAII CATG 3 cut(s) 351, 386, 759
CviJI RGCY 9 cut(s) 107, 129, 229, 414, 528, 588, 594, 728, 744
CviKI_1 RGCY 9 cut(s) 107, 129, 229, 414, 528, 588, 594, 728, 744
CviQI GTAC 1 cut(s) 148
DpnI GATC 3 cut(s) 356, 813, 819
DpnII GATC 3 cut(s) 354, 811, 817
EciI GGCGGA 2 cut(s) 221, 325
Ecl136II GAGCTC 2 cut(s) 229, 528
Eco130I CCWWGG 1 cut(s) 758
Eco24I GRGCYC 2 cut(s) 231, 530
Eco31I GGTCTC 1 cut(s) 150
Eco47I GGWCC 3 cut(s) 211, 312, 801
Eco53kI GAGCTC 2 cut(s) 229, 528
EcoICRI GAGCTC 2 cut(s) 229, 528
EcoRI GAATTC 1 cut(s) 685
EcoRII CCWGG 2 cut(s) 308, 571
EcoT14I CCWWGG 1 cut(s) 758
EcoT38I GRGCYC 2 cut(s) 231, 530
ErhI CCWWGG 1 cut(s) 758
FaeI CATG 3 cut(s) 354, 389, 762
FaiI YATR 7 cut(s) 71, 352, 387, 424, 426, 760, 782
FaqI GGGAC 2 cut(s) 754, 814
FatI CATG 3 cut(s) 350, 385, 758
FauI CCCGC 1 cut(s) 323
FbaI TGATCA 1 cut(s) 811
Fnu4HI GCNGC 3 cut(s) 27, 169, 172
FokI GGATG 2 cut(s) 180, 386
FriOI GRGCYC 2 cut(s) 231, 530
Fsp4HI GCNGC 3 cut(s) 27, 169, 172
FspBI CTAG 2 cut(s) 198, 585
GlaI GCGC 5 cut(s) 114, 139, 192, 384, 537
GluI GCNGC 3 cut(s) 27, 169, 172
GsaI CCCAGC 1 cut(s) 536
HaeII RGCGCY 1 cut(s) 539
HaeIII GGCC 3 cut(s) 107, 414, 744
HapII CCGG 5 cut(s) 185, 203, 294, 720, 745
HgaI GACGC 3 cut(s) 163, 244, 835
HhaI GCGC 5 cut(s) 115, 140, 193, 385, 538
Hin1I GRCGYC 2 cut(s) 118, 255
Hin1II CATG 3 cut(s) 354, 389, 762
Hin6I GCGC 5 cut(s) 113, 138, 191, 383, 536
HinP1I GCGC 5 cut(s) 113, 138, 191, 383, 536
HinfI GANTC 2 cut(s) 554, 560
HpaII CCGG 5 cut(s) 185, 203, 294, 720, 745
HphI GGTGA 1 cut(s) 775
Hpy188I TCNGA 3 cut(s) 420, 448, 559
Hpy188III TCNNGA 5 cut(s) 328, 432, 551, 698, 815
Hpy99I CGWCG 5 cut(s) 120, 123, 260, 263, 275
HpyAV CCTTC 4 cut(s) 118, 217, 296, 734
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 1 cut(s) 244
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 135, 389, 641
HpySE526I ACGT 1 cut(s) 118
Hsp92I GRCGYC 2 cut(s) 118, 255
Hsp92II CATG 3 cut(s) 354, 389, 762
HspAI GCGC 5 cut(s) 113, 138, 191, 383, 536
Ksp22I TGATCA 1 cut(s) 811
Kzo9I GATC 3 cut(s) 354, 811, 817
LmnI GCTCC 2 cut(s) 226, 533
Lsp1109I GCAGC 3 cut(s) 38, 180, 183
LweI GCATC 2 cut(s) 202, 439
MaeI CTAG 2 cut(s) 198, 585
MaeII ACGT 1 cut(s) 118
MalI GATC 3 cut(s) 356, 813, 819
MboI GATC 3 cut(s) 354, 811, 817
MboII GAAGA 4 cut(s) 232, 257, 447, 685
MflI RGATCY 1 cut(s) 817
MhlI GDGCHC 3 cut(s) 19, 231, 530
MluCI AATT 6 cut(s) 276, 358, 511, 623, 685, 853
MlyI GAGTC 1 cut(s) 548
MseI TTAA 3 cut(s) 279, 705, 822
MspCI CTTAAG 1 cut(s) 821
MspI CCGG 5 cut(s) 185, 203, 294, 720, 745
MspR9I CCNGG 2 cut(s) 310, 573
Mva1269I GAATGC 2 cut(s) 635, 829
MvaI CCWGG 2 cut(s) 310, 573
MvnI CGCG 3 cut(s) 113, 115, 140
MwoI GCNNNNNNNGC 4 cut(s) 23, 135, 389, 641
NcoI CCATGG 1 cut(s) 758
NdeII GATC 3 cut(s) 354, 811, 817
NlaIII CATG 3 cut(s) 354, 389, 762
NlaIV GGNNCC 2 cut(s) 314, 802
NspI RCATGY 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 2 cut(s) 635, 829
PfeI GAWTC 1 cut(s) 560
PkrI GCNGC 3 cut(s) 28, 170, 173
PleI GAGTC 1 cut(s) 548
PpsI GAGTC 1 cut(s) 548
Psp124BI GAGCTC 2 cut(s) 231, 530
Psp6I CCWGG 2 cut(s) 308, 571
PspFI CCCAGC 1 cut(s) 532
PspGI CCWGG 2 cut(s) 308, 571
PspN4I GGNNCC 2 cut(s) 314, 802
PspPI GGNCC 3 cut(s) 211, 312, 801
PstNI CAGNNNCTG 2 cut(s) 452, 508
PsuI RGATCY 1 cut(s) 817
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SacI GAGCTC 2 cut(s) 231, 530
SaqAI TTAA 3 cut(s) 279, 705, 822
SatI GCNGC 3 cut(s) 27, 169, 172
Sau3AI GATC 3 cut(s) 354, 811, 817
Sau96I GGNCC 3 cut(s) 211, 312, 801
SchI GAGTC 1 cut(s) 548
ScrFI CCNGG 2 cut(s) 310, 573
SduI GDGCHC 3 cut(s) 19, 231, 530
SfaNI GCATC 2 cut(s) 202, 439
SfcI CTRYAG 1 cut(s) 163
SinI GGWCC 3 cut(s) 211, 312, 801
SmlI CTYRAG 1 cut(s) 821
SmoI CTYRAG 1 cut(s) 821
Sse9I AATT 6 cut(s) 276, 358, 511, 623, 685, 853
SsiI CCGC 5 cut(s) 140, 206, 316, 336, 804
SspMI CTAG 2 cut(s) 198, 585
SstI GAGCTC 2 cut(s) 231, 530
StyD4I CCNGG 2 cut(s) 308, 571
StyI CCWWGG 1 cut(s) 758
TaaI ACNGT 1 cut(s) 241
TaiI ACGT 1 cut(s) 121
TaqI TCGA 1 cut(s) 273
TaqII GACCGA 2 cut(s) 27, 251
TasI AATT 6 cut(s) 276, 358, 511, 623, 685, 853
TatI WGTACW 1 cut(s) 147
TfiI GAWTC 1 cut(s) 560
Tru1I TTAA 3 cut(s) 279, 705, 822
Tru9I TTAA 3 cut(s) 279, 705, 822
TscAI CASTG 1 cut(s) 221
TseI GCWGC 3 cut(s) 26, 168, 171
TspRI CASTG 1 cut(s) 221
Vha464I CTTAAG 1 cut(s) 821
VpaK11BI GGWCC 3 cut(s) 211, 312, 801
XapI RAATTY 2 cut(s) 511, 685
XceI RCATGY 1 cut(s) 389
XcmI CCANNNNNNNNNTGG 1 cut(s) 657
XspI CTAG 2 cut(s) 198, 585
ZraI GACGTC 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.