Rh6BG322900

Protein OSB2, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012578
Physical Location & Seq
Reverse (-)
55399958 .. 55402033
2076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG322900.1

Sequence Viewer

Length: 867 bp
ATGGCGTTAGACCGAGCACTACAAGCAGCAACCTCCACAAACTTCCTCACAAACCCCCAAATCCCCTCTATATCCCCAAACACAACCACCACCACCCACAACCACTTGGCCTTCGCGCGACGTCGTTTCAGCTTCAAATGCGCGGTGGAGTACAGCAGAGACCACTACAGCAGCAGCGTCCAAGTTCCGGTGGCGCATCCTAGACCGGCGGAGGTCCAGTGGAAGAAGGAGCTCTGTAATACGGTGCAACTCATCGGCGTCGTCGGTCTTCCCGTCGAAATTAAGCACCTTCCCTCCGGTAAAGTCCTCGCCTGGACCCGCCTCGCCGTCAAGAAATCCGCCACCGACACCTCATGGATCAATTTGACATTTTGGGATGAGCTAGCGCATGTTGCTTTTCAGCATTTAGAGAAAGGCCATCAGATATATGTTTCTGGAAGACTGATTTCAGATGCTGTTGAAACCAATGAGGGGAAACAGCAGACCTACTATAAGGTTGTTGTTCAGCAACTGAATTTTGTTGAAAAGAGCTCCCCAACGGCGAATGGTGGTCAGGACTCGGATTCCTTTACACCAGGCAAAAGTTCTAGCCACAGCTTTACAAAGCACACAGAAACCACACAAGAATTGTGGCAAGCATTCTTTGCTAATCCAGTAGAGTGGTGGGATAATAGGAAGACCAAGAGGAATTCAAAATATCCAGATTTTAAGCACAAAGACACCGGAGAAGCCTTGTGGATTGAAGGCCGGAACAATCCTCCATGGGTGAAGTCCCAACTTGCTATACTGGATACAAGAATGGGGACCGCTAATGATCAAGATCTTAAGACGCATTCAAACCTGTTTGCTGATGATAATTTTTTCTAA

Protein Analysis

288

Amino Acids

32.66

Weight (kDa)

8.73

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 80 - 173 7e-17 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 124
AccII CGCG 3 cut(s) 116, 118, 143
AciI CCGC 5 cut(s) 143, 209, 319, 339, 807
AclWI GGATC 1 cut(s) 365
AcsI RAATTY 2 cut(s) 514, 688
AcyI GRCGYC 2 cut(s) 121, 258
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 2 cut(s) 187, 471
AflII CTTAAG 1 cut(s) 824
AgsI TTSAA 6 cut(s) 136, 461, 524, 693, 743, 837
AjnI CCWGG 2 cut(s) 311, 574
AluBI AGCT 5 cut(s) 132, 232, 382, 531, 597
AluI AGCT 5 cut(s) 132, 232, 382, 531, 597
Alw21I GWGCWC 3 cut(s) 19, 234, 533
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 1 cut(s) 365
AlwNI CAGNNNCTG 2 cut(s) 455, 511
AoxI GGCC 3 cut(s) 108, 415, 745
ApeKI GCWGC 3 cut(s) 26, 171, 174
ApoI RAATTY 2 cut(s) 514, 688
AspLEI GCGC 4 cut(s) 118, 143, 196, 388
AspS9I GGNCC 3 cut(s) 214, 315, 804
AsuHPI GGTGA 1 cut(s) 778
AsuNHI GCTAGC 1 cut(s) 382
AvaII GGWCC 3 cut(s) 214, 315, 804
BanII GRGCYC 2 cut(s) 234, 533
BbsI GAAGAC 3 cut(s) 260, 445, 683
Bbv12I GWGCWC 3 cut(s) 19, 234, 533
BbvI GCAGC 3 cut(s) 38, 183, 186
BccI CCATC 1 cut(s) 426
BceAI ACGGC 2 cut(s) 311, 555
BciT130I CCWGG 2 cut(s) 313, 576
BciVI GTATCC 1 cut(s) 784
BclI TGATCA 1 cut(s) 814
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 3 cut(s) 201, 383, 588
BfmI CTRYAG 1 cut(s) 166
BfrI CTTAAG 1 cut(s) 824
BfuI GTATCC 1 cut(s) 784
BglII AGATCT 1 cut(s) 820
BisI GCNGC 3 cut(s) 27, 172, 175
BlsI GCNGC 3 cut(s) 28, 173, 176
Bme1390I CCNGG 2 cut(s) 313, 576
Bme18I GGWCC 3 cut(s) 214, 315, 804
BmgT120I GGNCC 3 cut(s) 214, 315, 804
BmiI GGNNCC 2 cut(s) 317, 805
BmrFI CCNGG 2 cut(s) 313, 576
BmsI GCATC 2 cut(s) 205, 442
BmtI GCTAGC 1 cut(s) 386
BpiI GAAGAC 3 cut(s) 260, 445, 683
BsaBI GATNNNNATC 1 cut(s) 819
BsaHI GRCGYC 2 cut(s) 121, 258
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 761
BsaWI WCCGGW 3 cut(s) 187, 296, 722
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 278, 308
Bsc4I CCNNNNNNNGG 2 cut(s) 187, 471
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 3 cut(s) 217, 653, 792
Bse8I GATNNNNATC 1 cut(s) 819
BseBI CCWGG 2 cut(s) 313, 576
BseDI CCNNGG 1 cut(s) 761
BseGI GGATG 2 cut(s) 196, 382
BseJI GATNNNNATC 1 cut(s) 819
BseLI CCNNNNNNNGG 2 cut(s) 187, 471
BseNI ACTGG 3 cut(s) 217, 653, 792
BseXI GCAGC 3 cut(s) 38, 183, 186
Bsh1236I CGCG 3 cut(s) 116, 118, 143
BshFI GGCC 3 cut(s) 110, 417, 747
BsiHKAI GWGCWC 3 cut(s) 19, 234, 533
BsiSI CCGG 5 cut(s) 188, 206, 297, 723, 748
BslFI GGGAC 2 cut(s) 757, 817
BslI CCNNNNNNNGG 2 cut(s) 187, 471
BsmAI GTCTC 1 cut(s) 153
BsmFI GGGAC 2 cut(s) 757, 817
BsmI GAATGC 2 cut(s) 638, 832
BsnI GGCC 3 cut(s) 110, 417, 747
Bso31I GGTCTC 1 cut(s) 153
Bsp1286I GDGCHC 3 cut(s) 19, 234, 533
Bsp143I GATC 3 cut(s) 357, 814, 820
Bsp19I CCATGG 1 cut(s) 761
BspACI CCGC 5 cut(s) 143, 209, 319, 339, 807
BspANI GGCC 3 cut(s) 110, 417, 747
BspFNI CGCG 3 cut(s) 116, 118, 143
BspLI GGNNCC 2 cut(s) 317, 805
BspOI GCTAGC 1 cut(s) 386
BspPI GGATC 1 cut(s) 365
BspTI CTTAAG 1 cut(s) 824
BspTNI GGTCTC 1 cut(s) 153
BsrFI RCCGGY 1 cut(s) 205
BsrI ACTGG 3 cut(s) 217, 653, 792
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 1 cut(s) 761
BssMI GATC 3 cut(s) 357, 814, 820
BssNI GRCGYC 2 cut(s) 121, 258
BssT1I CCWWGG 1 cut(s) 761
Bst2UI CCWGG 2 cut(s) 313, 576
Bst4CI ACNGT 1 cut(s) 244
BstACI GRCGYC 2 cut(s) 121, 258
BstAFI CTTAAG 1 cut(s) 824
BstAPI GCANNNNNTGC 1 cut(s) 644
BstC8I GCNNGC 2 cut(s) 384, 636
BstDSI CCRYGG 1 cut(s) 761
BstF5I GGATG 2 cut(s) 196, 382
BstFNI CGCG 3 cut(s) 116, 118, 143
BstHHI GCGC 4 cut(s) 118, 143, 196, 388
BstKTI GATC 3 cut(s) 360, 817, 823
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 3 cut(s) 357, 814, 820
BstMWI GCNNNNNNNGC 4 cut(s) 23, 138, 392, 644
BstNI CCWGG 2 cut(s) 313, 576
BstNSI RCATGY 1 cut(s) 392
BstSCI CCNGG 2 cut(s) 311, 574
BstSFI CTRYAG 1 cut(s) 166
BstUI CGCG 3 cut(s) 116, 118, 143
BstV1I GCAGC 3 cut(s) 38, 183, 186
BstV2I GAAGAC 3 cut(s) 260, 445, 683
BstX2I RGATCY 1 cut(s) 820
BstXI CCANNNNNNTGG 1 cut(s) 660
BstYI RGATCY 1 cut(s) 820
BsuI GTATCC 1 cut(s) 784
BsuRI GGCC 3 cut(s) 110, 417, 747
BtgI CCRYGG 1 cut(s) 761
BtsCI GGATG 2 cut(s) 196, 382
BtsIMutI CAGTG 1 cut(s) 224
Cac8I GCNNGC 2 cut(s) 384, 636
CaiI CAGNNNCTG 2 cut(s) 455, 511
CfoI GCGC 4 cut(s) 118, 143, 196, 388
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 3 cut(s) 214, 315, 804
CseI GACGC 3 cut(s) 166, 247, 838
Csp6I GTAC 1 cut(s) 151
CspCI CAANNNNNGTGG 2 cut(s) 611, 646
CviAII CATG 3 cut(s) 354, 389, 762
CviQI GTAC 1 cut(s) 151
DpnI GATC 3 cut(s) 359, 816, 822
DpnII GATC 3 cut(s) 357, 814, 820
EciI GGCGGA 2 cut(s) 224, 328
Ecl136II GAGCTC 2 cut(s) 232, 531
Eco130I CCWWGG 1 cut(s) 761
Eco24I GRGCYC 2 cut(s) 234, 533
Eco31I GGTCTC 1 cut(s) 153
Eco47I GGWCC 3 cut(s) 214, 315, 804
Eco53kI GAGCTC 2 cut(s) 232, 531
EcoICRI GAGCTC 2 cut(s) 232, 531
EcoRI GAATTC 1 cut(s) 688
EcoRII CCWGG 2 cut(s) 311, 574
EcoT14I CCWWGG 1 cut(s) 761
EcoT38I GRGCYC 2 cut(s) 234, 533
ErhI CCWWGG 1 cut(s) 761
FaeI CATG 3 cut(s) 357, 392, 765
FaiI YATR 8 cut(s) 71, 355, 390, 427, 429, 492, 763, 785
FaqI GGGAC 2 cut(s) 757, 817
FatI CATG 3 cut(s) 353, 388, 761
FauI CCCGC 1 cut(s) 326
FbaI TGATCA 1 cut(s) 814
Fnu4HI GCNGC 3 cut(s) 27, 172, 175
FokI GGATG 2 cut(s) 183, 389
FriOI GRGCYC 2 cut(s) 234, 533
Fsp4HI GCNGC 3 cut(s) 27, 172, 175
FspBI CTAG 3 cut(s) 201, 383, 588
GlaI GCGC 4 cut(s) 117, 142, 195, 387
GluI GCNGC 3 cut(s) 27, 172, 175
HaeIII GGCC 3 cut(s) 110, 417, 747
HapII CCGG 5 cut(s) 188, 206, 297, 723, 748
HgaI GACGC 3 cut(s) 166, 247, 838
HhaI GCGC 4 cut(s) 118, 143, 196, 388
Hin1I GRCGYC 2 cut(s) 121, 258
Hin1II CATG 3 cut(s) 357, 392, 765
Hin6I GCGC 4 cut(s) 116, 141, 194, 386
HinP1I GCGC 4 cut(s) 116, 141, 194, 386
HinfI GANTC 2 cut(s) 557, 563
HpaII CCGG 5 cut(s) 188, 206, 297, 723, 748
HphI GGTGA 1 cut(s) 778
Hpy188I TCNGA 3 cut(s) 423, 451, 562
Hpy188III TCNNGA 5 cut(s) 331, 435, 554, 701, 818
Hpy99I CGWCG 5 cut(s) 123, 126, 263, 266, 278
HpyAV CCTTC 4 cut(s) 121, 220, 299, 737
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 1 cut(s) 121
HpyCH4V TGCA 1 cut(s) 247
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 138, 392, 644
HpySE526I ACGT 1 cut(s) 121
Hsp92I GRCGYC 2 cut(s) 121, 258
Hsp92II CATG 3 cut(s) 357, 392, 765
HspAI GCGC 4 cut(s) 116, 141, 194, 386
Ksp22I TGATCA 1 cut(s) 814
Kzo9I GATC 3 cut(s) 357, 814, 820
LmnI GCTCC 2 cut(s) 229, 536
Lsp1109I GCAGC 3 cut(s) 38, 183, 186
LweI GCATC 2 cut(s) 205, 442
MaeI CTAG 3 cut(s) 201, 383, 588
MaeII ACGT 1 cut(s) 121
MalI GATC 3 cut(s) 359, 816, 822
MboI GATC 3 cut(s) 357, 814, 820
MboII GAAGA 4 cut(s) 235, 260, 450, 688
MflI RGATCY 1 cut(s) 820
MhlI GDGCHC 3 cut(s) 19, 234, 533
MluCI AATT 6 cut(s) 279, 361, 514, 626, 688, 856
MlyI GAGTC 1 cut(s) 551
MseI TTAA 3 cut(s) 282, 708, 825
MspCI CTTAAG 1 cut(s) 824
MspI CCGG 5 cut(s) 188, 206, 297, 723, 748
MspR9I CCNGG 2 cut(s) 313, 576
Mva1269I GAATGC 2 cut(s) 638, 832
MvaI CCWGG 2 cut(s) 313, 576
MvnI CGCG 3 cut(s) 116, 118, 143
MwoI GCNNNNNNNGC 4 cut(s) 23, 138, 392, 644
NcoI CCATGG 1 cut(s) 761
NdeII GATC 3 cut(s) 357, 814, 820
NheI GCTAGC 1 cut(s) 382
NlaIII CATG 3 cut(s) 357, 392, 765
NlaIV GGNNCC 2 cut(s) 317, 805
NspI RCATGY 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 2 cut(s) 638, 832
PfeI GAWTC 1 cut(s) 563
PkrI GCNGC 3 cut(s) 28, 173, 176
PleI GAGTC 1 cut(s) 551
PpsI GAGTC 1 cut(s) 551
Psp124BI GAGCTC 2 cut(s) 234, 533
Psp6I CCWGG 2 cut(s) 311, 574
PspGI CCWGG 2 cut(s) 311, 574
PspN4I GGNNCC 2 cut(s) 317, 805
PspPI GGNCC 3 cut(s) 214, 315, 804
PstNI CAGNNNCTG 2 cut(s) 455, 511
PsuI RGATCY 1 cut(s) 820
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
SacI GAGCTC 2 cut(s) 234, 533
SaqAI TTAA 3 cut(s) 282, 708, 825
SatI GCNGC 3 cut(s) 27, 172, 175
Sau3AI GATC 3 cut(s) 357, 814, 820
Sau96I GGNCC 3 cut(s) 214, 315, 804
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 2 cut(s) 313, 576
SduI GDGCHC 3 cut(s) 19, 234, 533
SfaNI GCATC 2 cut(s) 205, 442
SfcI CTRYAG 1 cut(s) 166
SinI GGWCC 3 cut(s) 214, 315, 804
SmlI CTYRAG 1 cut(s) 824
SmoI CTYRAG 1 cut(s) 824
Sse9I AATT 6 cut(s) 279, 361, 514, 626, 688, 856
SsiI CCGC 5 cut(s) 143, 209, 319, 339, 807
SspMI CTAG 3 cut(s) 201, 383, 588
SstI GAGCTC 2 cut(s) 234, 533
StyD4I CCNGG 2 cut(s) 311, 574
StyI CCWWGG 1 cut(s) 761
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 1 cut(s) 124
TaqI TCGA 1 cut(s) 276
TaqII GACCGA 2 cut(s) 27, 254
TasI AATT 6 cut(s) 279, 361, 514, 626, 688, 856
TatI WGTACW 1 cut(s) 150
TfiI GAWTC 1 cut(s) 563
Tru1I TTAA 3 cut(s) 282, 708, 825
Tru9I TTAA 3 cut(s) 282, 708, 825
TscAI CASTG 1 cut(s) 224
TseI GCWGC 3 cut(s) 26, 171, 174
TspRI CASTG 1 cut(s) 224
Vha464I CTTAAG 1 cut(s) 824
VpaK11BI GGWCC 3 cut(s) 214, 315, 804
XapI RAATTY 2 cut(s) 514, 688
XceI RCATGY 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 660
XspI CTAG 3 cut(s) 201, 383, 588
ZraI GACGTC 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.