Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0016717

Physical Location & Seq

Genomic Coordinates Reverse (-)

32351778 .. 32354495

Gene Structure 2718 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1226400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2718 bp

ATGTCCTCCTCCTACACTCAACTGCTCACACCTCTCTCTGGCCCCTTACCTTCCCAACCACTTTCTGCCCTCCAAATCCACCAACCCACAACTCTCAAAACACACACTCTAAAGCCCTCTCCTTTACTCAACCCACCCACCACCACCACGGGCGGCGGTTCTGCACCGAAACTCGCATCCCACTCCTCTCGCACTCCGGCCACATGGGTCGAGACCCTCCGCTCCCAAACCCGGTCCAATCATTTCCGAGAAGCCATTTCTACGTACATCGAAATGACTCTTTCAGGTGTGGCACCCGACAACTTTGCTTTCCCTGCAGTTCTGAAGGCCGTCACGGGGATTCAGGACTTGAATTTGGGGAAACAGATTCATGCCCATGTTGTGAAATTCGGGTATGGGTTATCCTCGGTGACCGTGGCTAATACCCTTGTAAATGTGTATGGGAAATGTGGAGATATTGGAGATGCATGCAAAGTGTTCGATGGAATAACTGACAGAGACCAAGTTTCTTGGAATTCCATGATTGCTGCGTTGTGTCGGATCGAGGAATGGGAACTCGCTCTCGATGCGTTTAGGTCGATGTTATTGGAGAATATGGAGCCTAGCTCCTTTACTTTGGTGAGTGTGGCACATGCTTGTTCGAATTTGCATAAGCGTGATGGGTTGCGGCTTGGGAAGCAAGTTCATGCGTATGGTCTGAGAGTGAGTGATTGGAAAACATTTACAATCAATGCTTTATTGGCAATGTATGCTAAATTAGGATTAGTTGAGTACTCGAGAGCTTTGTTTGAGATGTTTGAGGACTGTGACATGGTTTCGTGGAACACAATGATAAGTTCGTTCTCTCAGAACGACCAGTTTATGGAAGCATTAGAGTTCTTCCGCCTTATGGTTCTTGCAGGACTCAAACCAGATGGGGTTACAGTTGCAAGTGTTCTTCCGGCATGTTCTCATCTGGAGATGTTAGATACAGGGAAGGAAATTCATGCTTATGCCTTAAGAACCACTGAATTAAGTGAAAATTCTTATGTTGGTAGTGCTTTAGTTGACATGTATTGCAACTGCCGACAAGTTGGAAGTGGTCGTCAAGTTTTCGACGCTGTCTTGGAGCGGAAAATTCCACTTTGGAATGCAATGATTACTGGCTATGCACAAAATGAGTACGATGAAGAGGCATTGAAACTGTTCCTTGAAATGTATGCTGCCTCCGCGCTTTTCCCAAATTCCACCACAATGTCGAGTATTGTGCCAGCCTGTGTGCGGTCTGAATCATTCGCTGATAAAGAGAGTATCCACGGTTATGTAATAAAGAGGGGTCTGGAGAAGAACAGGTACGTGCAAAATGCACTTGTGGACATGTACTCCAGGTTAGGAAAGACAGTAATTTCAGAAATCATATTTAACAGCATGGAGGCAAAAGACATAGTGTCGTGGAATACCATGATCACTGGTTATGTTATTTCTGGACGCCATGGTGATGCGCTTAATCTGCTATGTGATATGCAAAGAGTAGAAGAAAAGAAGAACACAGATTATACTGGTTATGACAATGAAAAAAGTATTCCTCTTAAACCAAATTCAATAACATTCATGACAATTCTTCCTGGTTGTGCTGCACTGGGAGCACTCGCTAAGGGGAAAGAGATCCATGCTTATGCTATTAGAAACTTATTGGCATTTGATGTTGCAGTAGGAAGTGCGCTGGTTGACATGTATGCGAAATGTGGCTGCATAGGTTTAGCTAGAACTGTGTTTAATCAGATACCAATAAAGAATGTGATCACCTGGAATGTACTTATCATGGCTTATGGAATGCAAGGGAGAGGGGAGGAAGCATTAGAACTACTTAGGGATATGGTGGATGAAGGAAGACGAAACAAAGAGGTAAGGCCTAATGAAGTTACGTTCATTGCACTTTTTGCTGCGTGTAGTCATTCTGGGATGGTTGATGAAGGCCTGAATTTGTTCCGAAAAATGAAAGAGGACTATGGAATTGAACCTGCACCAGATCATTATGCTTGTGTTGTAGACTTGCTTGGTCGGGCTGGTAATGTGGAGGAAGCATATCAATTCATCAACTCTATGCCTTCTCAACTCGATAAAGCAGGTGCTTGGAGTAGCTTGCTTGGTGCCTGTCGAATTCACCAGAATGTAGAGATTGGAGAAGTTGCAGCTAATCATCTCCTGCAGTTGGAGCCTGATGTGGCTAGTCACTACGTTCTACTTTCTAATATCTACTCATCTTCCGGATTCTGGGAAAAGGCAATGGATGTTCGGAGAAAGATGAGGGAATTGGGAGTGAGAAAAGAACCTGGTTATAGTTGGATTGAGTTTGGCGATGAGGTTCATAAGTTTTTGGCTGGAGATTTGTCACACCAACAAAGCAGGCAGCTCCATGAATTTCTCGAAACCCTGTCAGAAAAGATGAAAAGGGAGGGTTATGTGCCTGATACTTCTTGTGTACTTCACAATGTTGACGAGGAGGAGAAAGAAACTCTACTCTGTGGGCATAGCGAGAAACTGGCAATAGCGTTTGGCATTCTCAATACCCGTCCCGGGACTACCATTAGAGTTTCCAAGAACCTTCGAGTTTGTAATGACTGCCACACTGCGTCAAAGTATATCTCAAAGATTACGGACAGGGAGATCATTCTAAGAGACGTAAGAAGGTTCCATCACTTTAAAAATGGAACTTGTTCATGCGGAGATTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

906

Amino Acids

100.74

Weight (kDa)

6.06

Isoelectric Point (pI)

42.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	167 - 214	3.9e-08	PPR repeat family
PPR_2	PF13041	270 - 317	2.9e-08	PPR repeat family
PPR	PF01535	272 - 301	1.1e-06	PPR repeat
PPR_2	PF13041	473 - 503	4.3e-06	PPR repeat family
PPR_2	PF13041	591 - 623	1.6e-06	PPR repeat family
PPR	PF01535	594 - 623	1.7e-07	PPR repeat
E_motif	PF20431	715 - 777	3.5e-18	E motif
DYW_deaminase	PF14432	813 - 905	1.3e-45	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0016717)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57430
fragaria_vesca	FvH4_3g13090
malus_domestica	MD10G1226400.v1.1
prunus_persica	Prupe.1G101200_v2.0.a1
pyrus_communis	pycom10g19030
rosa_chinensis	RchiOBHm_Chr5g0021321
rosa_laevigata	RLG00000032604
rosa_multiflora	Rmu_co8520529.1_g000003 Rmu_sc0003392.1_g000007 Rmu_sc0004847.1_g000016
rosa_rugosa	Rorug05G0066300
rosa_samantha	Rh5BG154900 Rh5CG168400
rosa_wichuraiana	Rw5G013850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2096
Acc36I	ACCTGC	2 cut(s)	2008, 2096
AccB1I	GGYRCC	2 cut(s)	292, 2129
AccB7I	CCANNNNNTGG	1 cut(s)	862
AccBSI	CCGCTC	2 cut(s)	222, 1111
AccI	GTMKAC	1 cut(s)	2028
AccII	CGCG	1 cut(s)	1211
AccIII	TCCGGA	1 cut(s)	2246
AciI	CCGC	9 cut(s)	153, 156, 220, 667, 883, 1111, 1209, 1261, 2703
AclWI	GGATC	2 cut(s)	548, 1639
AcoI	YGGCCR	1 cut(s)	198
AcuI	CTGAAG	1 cut(s)	344
AcyI	GRCGYC	1 cut(s)	1468
AfaI	GTAC	7 cut(s)	266, 773, 1163, 1334, 1361, 1794, 2463
AfiI	CCNNNNNNNGG	9 cut(s)	38, 231, 336, 862, 889, 1260, 2191, 2253, 2556
AflII	CTTAAG	1 cut(s)	997
AflIII	ACRYGT	3 cut(s)	1050, 1356, 1710
AgsI	TTSAA	5 cut(s)	352, 1180, 1193, 1581, 1997
AhdI	GACNNNNNGTC	1 cut(s)	1426
AjnI	CCWGG	4 cut(s)	1364, 1603, 1784, 2311
AluBI	AGCT	6 cut(s)	606, 782, 1742, 2121, 2174, 2392
AluI	AGCT	6 cut(s)	606, 782, 1742, 2121, 2174, 2392
Alw21I	GWGCWC	1 cut(s)	1627
Alw26I	GTCTC	3 cut(s)	206, 492, 2652
AlwI	GGATC	2 cut(s)	548, 1639
Ama87I	CYCGRG	2 cut(s)	775, 2555
Aor13HI	TCCGGA	1 cut(s)	2246
AoxI	GGCC	5 cut(s)	40, 198, 327, 1889, 1954
ApeKI	GCWGC	7 cut(s)	527, 1202, 1613, 1728, 1922, 2171, 2389
Asp700I	GAANNNNTTC	3 cut(s)	1184, 1964, 2695
AspLEI	GCGC	3 cut(s)	1213, 1483, 1702
AspS9I	GGNCC	2 cut(s)	41, 234
AsuC2I	CCSGG	3 cut(s)	232, 2556, 2557
AsuHPI	GGTGA	5 cut(s)	421, 631, 1487, 1774, 2135
AsuII	TTCGAA	1 cut(s)	641
AvaI	CYCGRG	2 cut(s)	775, 2555
AvaII	GGWCC	1 cut(s)	234
BanI	GGYRCC	2 cut(s)	292, 2129
BarI	GAAGNNNNNNTAC	2 cut(s)	1316, 1348
BbsI	GAAGAC	1 cut(s)	1876
Bbv12I	GWGCWC	1 cut(s)	1627
BbvI	GCAGC	7 cut(s)	514, 1189, 1600, 1715, 1909, 2183, 2401
BccI	CCATC	5 cut(s)	476, 653, 908, 1936, 2682
BceAI	ACGGC	1 cut(s)	314
BcgI	CGANNNNNNTGC	6 cut(s)	287, 321, 460, 494, 1228, 1262
BciT130I	CCWGG	4 cut(s)	1366, 1605, 1786, 2313
BciVI	GTATCC	1 cut(s)	1301
BclI	TGATCA	2 cut(s)	1443, 1779
BcnI	CCSGG	3 cut(s)	232, 2556, 2557
BcoDI	GTCTC	3 cut(s)	206, 492, 2652
BfaI	CTAG	3 cut(s)	603, 1743, 2208
BfmI	CTRYAG	2 cut(s)	315, 2186
BfrI	CTTAAG	1 cut(s)	997
BfuAI	ACCTGC	2 cut(s)	2008, 2096
BfuI	GTATCC	1 cut(s)	1301
BisI	GCNGC	9 cut(s)	154, 528, 668, 1203, 1614, 1729, 1923, 2172, 2390
BlsI	GCNGC	9 cut(s)	155, 529, 669, 1204, 1615, 1730, 1924, 2173, 2391
BmcAI	AGTACT	1 cut(s)	773
Bme1390I	CCNGG	7 cut(s)	232, 1366, 1605, 1786, 2313, 2556, 2557
Bme18I	GGWCC	1 cut(s)	234
BmeRI	GACNNNNNGTC	1 cut(s)	1426
BmeT110I	CYCGRG	2 cut(s)	775, 2555
BmgT120I	GGNCC	2 cut(s)	41, 234
BmiI	GGNNCC	6 cut(s)	43, 294, 600, 2131, 2196, 2672
BmrFI	CCNGG	7 cut(s)	232, 1366, 1605, 1786, 2313, 2556, 2557
BmrI	ACTGGG	1 cut(s)	1628
BmsI	GCATC	4 cut(s)	185, 454, 556, 1468
BmuI	ACTGGG	1 cut(s)	1628
BpiI	GAAGAC	1 cut(s)	1876
BplI	GAGNNNNNCTC	2 cut(s)	590, 622
BpmI	CTGGAG	4 cut(s)	977, 1340, 1348, 2382
Bpu10I	CCTNAGC	1 cut(s)	1632
Bpu14I	TTCGAA	1 cut(s)	641
BpuMI	CCSGG	3 cut(s)	232, 2556, 2557
BsaAI	YACGTR	2 cut(s)	264, 1336
BsaHI	GRCGYC	1 cut(s)	1468
BsaI	GGTCTC	2 cut(s)	206, 492
BsaJI	CCNNGG	6 cut(s)	147, 405, 414, 1294, 1471, 2555
BsaWI	WCCGGW	1 cut(s)	2246
BsaXI	ACNNNNNCTCC	2 cut(s)	1346, 1376
Bsc4I	CCNNNNNNNGG	9 cut(s)	38, 231, 336, 862, 889, 1260, 2191, 2253, 2556
Bse1I	ACTGG	6 cut(s)	856, 1147, 1453, 1543, 1623, 2526
Bse3DI	GCAATG	4 cut(s)	750, 1140, 1908, 2271
BseAI	TCCGGA	1 cut(s)	2246
BseBI	CCWGG	4 cut(s)	1366, 1605, 1786, 2313
BseDI	CCNNGG	6 cut(s)	147, 405, 414, 1294, 1471, 2555
BseGI	GGATG	4 cut(s)	176, 1867, 1947, 2275
BseLI	CCNNNNNNNGG	9 cut(s)	38, 231, 336, 862, 889, 1260, 2191, 2253, 2556
BseMI	GCAATG	4 cut(s)	750, 1140, 1908, 2271
BseMII	CTCAG	2 cut(s)	689, 860
BseNI	ACTGG	6 cut(s)	856, 1147, 1453, 1543, 1623, 2526
BseRI	GAGGAG	3 cut(s)	175, 2495, 2498
BseXI	GCAGC	7 cut(s)	514, 1189, 1600, 1715, 1909, 2183, 2401
BsgI	GTGCAG	3 cut(s)	147, 1599, 1986
Bsh1236I	CGCG	1 cut(s)	1211
BshFI	GGCC	5 cut(s)	42, 200, 329, 1891, 1956
BshNI	GGYRCC	2 cut(s)	292, 2129
BsiHKAI	GWGCWC	1 cut(s)	1627
BsiHKCI	CYCGRG	2 cut(s)	775, 2555
BsiSI	CCGG	5 cut(s)	197, 232, 941, 2247, 2556
BslFI	GGGAC	2 cut(s)	2538, 2572
BslI	CCNNNNNNNGG	9 cut(s)	38, 231, 336, 862, 889, 1260, 2191, 2253, 2556
BsmAI	GTCTC	3 cut(s)	206, 492, 2652
BsmBI	CGTCTC	1 cut(s)	2652
BsmFI	GGGAC	2 cut(s)	2538, 2572
BsmI	GAATGC	3 cut(s)	1135, 1818, 2538
BsnI	GGCC	5 cut(s)	42, 200, 329, 1891, 1956
Bso31I	GGTCTC	2 cut(s)	206, 492
BsoBI	CYCGRG	2 cut(s)	775, 2555
Bsp119I	TTCGAA	1 cut(s)	641
Bsp1286I	GDGCHC	1 cut(s)	1627
Bsp13I	TCCGGA	1 cut(s)	2246
Bsp143I	GATC	6 cut(s)	540, 1443, 1644, 1779, 2008, 2646
Bsp19I	CCATGG	1 cut(s)	1471
BspACI	CCGC	9 cut(s)	153, 156, 220, 667, 883, 1111, 1209, 1261, 2703
BspANI	GGCC	5 cut(s)	42, 200, 329, 1891, 1956
BspCNI	CTCAG	2 cut(s)	690, 859
BspEI	TCCGGA	1 cut(s)	2246
BspFNI	CGCG	1 cut(s)	1211
BspHI	TCATGA	1 cut(s)	1590
BspLI	GGNNCC	6 cut(s)	43, 294, 600, 2131, 2196, 2672
BspMAI	CTGCAG	2 cut(s)	319, 2190
BspMI	ACCTGC	2 cut(s)	2008, 2096
BspPI	GGATC	2 cut(s)	548, 1639
BspT104I	TTCGAA	1 cut(s)	641
BspT107I	GGYRCC	2 cut(s)	292, 2129
BspTI	CTTAAG	1 cut(s)	997
BspTNI	GGTCTC	2 cut(s)	206, 492
BsrBI	CCGCTC	2 cut(s)	222, 1111
BsrDI	GCAATG	4 cut(s)	750, 1140, 1908, 2271
BsrI	ACTGG	6 cut(s)	856, 1147, 1453, 1543, 1623, 2526
BssECI	CCNNGG	6 cut(s)	147, 405, 414, 1294, 1471, 2555
BssMI	GATC	6 cut(s)	540, 1443, 1644, 1779, 2008, 2646
BssNI	GRCGYC	1 cut(s)	1468
BssT1I	CCWWGG	1 cut(s)	1471
Bst2UI	CCWGG	4 cut(s)	1366, 1605, 1786, 2313
Bst4CI	ACNGT	7 cut(s)	415, 806, 925, 1185, 1298, 1381, 1750
Bst6I	CTCTTC	1 cut(s)	1164
BstACI	GRCGYC	1 cut(s)	1468
BstAFI	CTTAAG	1 cut(s)	997
BstAPI	GCANNNNNTGC	2 cut(s)	749, 1919
BstBAI	YACGTR	2 cut(s)	264, 1336
BstBI	TTCGAA	1 cut(s)	641
BstC8I	GCNNGC	4 cut(s)	469, 1251, 2123, 2387
BstDEI	CTNAG	5 cut(s)	698, 846, 1632, 1847, 2654
BstDSI	CCRYGG	4 cut(s)	147, 414, 1294, 1471
BstEII	GGTNACC	1 cut(s)	409
BstF5I	GGATG	4 cut(s)	176, 1867, 1947, 2275
BstFNI	CGCG	1 cut(s)	1211
BstHHI	GCGC	3 cut(s)	1213, 1483, 1702
BstKTI	GATC	6 cut(s)	543, 1446, 1647, 1782, 2011, 2649
BstMAI	GTCTC	3 cut(s)	206, 492, 2652
BstMBI	GATC	6 cut(s)	540, 1443, 1644, 1779, 2008, 2646
BstNI	CCWGG	4 cut(s)	1366, 1605, 1786, 2313
BstNSI	RCATGY	6 cut(s)	471, 635, 948, 1054, 1360, 1714
BstPI	GGTNACC	1 cut(s)	409
BstSCI	CCNGG	7 cut(s)	230, 1364, 1603, 1784, 2311, 2554, 2555
BstSFI	CTRYAG	2 cut(s)	315, 2186
BstSNI	TACGTA	1 cut(s)	264
BstUI	CGCG	1 cut(s)	1211
BstV1I	GCAGC	7 cut(s)	514, 1189, 1600, 1715, 1909, 2183, 2401
BstV2I	GAAGAC	1 cut(s)	1876
BstX2I	RGATCY	1 cut(s)	1644
BstYI	RGATCY	1 cut(s)	1644
BsuI	GTATCC	1 cut(s)	1301
BsuRI	GGCC	5 cut(s)	42, 200, 329, 1891, 1956
BtgI	CCRYGG	4 cut(s)	147, 414, 1294, 1471
BtgZI	GCGATG	1 cut(s)	2352
BtsCI	GGATG	4 cut(s)	176, 1867, 1947, 2275
BtsI	GCAGTG	1 cut(s)	2607
BtsIMutI	CAGTG	4 cut(s)	1005, 1446, 1616, 2607
BveI	ACCTGC	2 cut(s)	2008, 2096
Cac8I	GCNNGC	4 cut(s)	469, 1251, 2123, 2387
CciI	TCATGA	1 cut(s)	1590
CfoI	GCGC	3 cut(s)	1213, 1483, 1702
Cfr13I	GGNCC	2 cut(s)	41, 234
Cfr9I	CCCGGG	1 cut(s)	2555
CseI	GACGC	3 cut(s)	1106, 1476, 2601
CsiI	ACCWGGT	1 cut(s)	2311
Csp6I	GTAC	7 cut(s)	265, 772, 1162, 1333, 1360, 1793, 2462
CviQI	GTAC	7 cut(s)	265, 772, 1162, 1333, 1360, 1793, 2462
DdeI	CTNAG	5 cut(s)	698, 846, 1632, 1847, 2654
DpnI	GATC	6 cut(s)	542, 1445, 1646, 1781, 2010, 2648
DpnII	GATC	6 cut(s)	540, 1443, 1644, 1779, 2008, 2646
DraI	TTTAAA	1 cut(s)	2683
DriI	GACNNNNNGTC	1 cut(s)	1426
EaeI	YGGCCR	1 cut(s)	198
Eam1104I	CTCTTC	1 cut(s)	1164
Eam1105I	GACNNNNNGTC	1 cut(s)	1426
EarI	CTCTTC	1 cut(s)	1164
EciI	GGCGGA	1 cut(s)	872
Eco105I	TACGTA	1 cut(s)	264
Eco130I	CCWWGG	1 cut(s)	1471
Eco147I	AGGCCT	2 cut(s)	1891, 1956
Eco31I	GGTCTC	2 cut(s)	206, 492
Eco47I	GGWCC	1 cut(s)	234
Eco57I	CTGAAG	1 cut(s)	344
Eco88I	CYCGRG	2 cut(s)	775, 2555
Eco91I	GGTNACC	1 cut(s)	409
EcoO65I	GGTNACC	1 cut(s)	409
EcoRI	GAATTC	2 cut(s)	514, 2139
EcoRII	CCWGG	4 cut(s)	1364, 1603, 1784, 2311
EcoT14I	CCWWGG	1 cut(s)	1471
EcoT22I	ATGCAT	1 cut(s)	469
ErhI	CCWWGG	1 cut(s)	1471
Esp3I	CGTCTC	1 cut(s)	2652
FaqI	GGGAC	2 cut(s)	2538, 2572
FbaI	TGATCA	2 cut(s)	1443, 1779
FblI	GTMKAC	1 cut(s)	2028
Fnu4HI	GCNGC	9 cut(s)	154, 528, 668, 1203, 1614, 1729, 1923, 2172, 2390
FokI	GGATG	4 cut(s)	163, 1874, 1954, 2282
Fsp4HI	GCNGC	9 cut(s)	154, 528, 668, 1203, 1614, 1729, 1923, 2172, 2390
FspBI	CTAG	3 cut(s)	603, 1743, 2208
GlaI	GCGC	3 cut(s)	1212, 1482, 1701
GluI	GCNGC	9 cut(s)	154, 528, 668, 1203, 1614, 1729, 1923, 2172, 2390
GsuI	CTGGAG	4 cut(s)	977, 1340, 1348, 2382
HaeIII	GGCC	5 cut(s)	42, 200, 329, 1891, 1956
HapII	CCGG	5 cut(s)	197, 232, 941, 2247, 2556
HgaI	GACGC	3 cut(s)	1106, 1476, 2601
HhaI	GCGC	3 cut(s)	1213, 1483, 1702
Hin1I	GRCGYC	1 cut(s)	1468
Hin6I	GCGC	3 cut(s)	1211, 1481, 1700
HinP1I	GCGC	3 cut(s)	1211, 1481, 1700
HincII	GTYRAC	3 cut(s)	1048, 1708, 2476
HindII	GTYRAC	3 cut(s)	1048, 1708, 2476
HinfI	GANTC	6 cut(s)	277, 340, 367, 903, 1268, 2250
HpaII	CCGG	5 cut(s)	197, 232, 941, 2247, 2556
HphI	GGTGA	5 cut(s)	421, 631, 1487, 1774, 2135
Hpy166II	GTNNAC	6 cut(s)	1048, 1354, 1708, 2029, 2462, 2476
Hpy8I	GTNNAC	6 cut(s)	1048, 1354, 1708, 2029, 2462, 2476
Hpy99I	CGWCG	1 cut(s)	1100
HpyAV	CCTTC	8 cut(s)	60, 319, 970, 1859, 1946, 2097, 2594, 2661
HpyCH4III	ACNGT	7 cut(s)	415, 806, 925, 1185, 1298, 1381, 1750
HpyCH4IV	ACGT	5 cut(s)	263, 1335, 1904, 2217, 2661
HpyF3I	CTNAG	5 cut(s)	698, 846, 1632, 1847, 2654
HpySE526I	ACGT	5 cut(s)	263, 1335, 1904, 2217, 2661
Hsp92I	GRCGYC	1 cut(s)	1468
HspAI	GCGC	3 cut(s)	1211, 1481, 1700
Kpn2I	TCCGGA	1 cut(s)	2246
Ksp22I	TGATCA	2 cut(s)	1443, 1779
Kzo9I	GATC	6 cut(s)	540, 1443, 1644, 1779, 2008, 2646
LmnI	GCTCC	7 cut(s)	227, 598, 611, 1108, 1622, 2194, 2397
Lsp1109I	GCAGC	7 cut(s)	514, 1189, 1600, 1715, 1909, 2183, 2401
LweI	GCATC	4 cut(s)	185, 454, 556, 1468
MabI	ACCWGGT	1 cut(s)	2311
MaeI	CTAG	3 cut(s)	603, 1743, 2208
MaeII	ACGT	5 cut(s)	263, 1335, 1904, 2217, 2661
MaeIII	GTNAC	7 cut(s)	331, 409, 806, 919, 1900, 2210, 2370
MalI	GATC	6 cut(s)	542, 1445, 1646, 1781, 2010, 2648
MbiI	CCGCTC	2 cut(s)	222, 1111
MboI	GATC	6 cut(s)	540, 1443, 1644, 1779, 2008, 2646
MboII	GAAGA	9 cut(s)	871, 929, 1181, 1336, 1526, 1534, 1592, 1881, 2235
MflI	RGATCY	1 cut(s)	1644
MhlI	GDGCHC	1 cut(s)	1627
MlyI	GAGTC	2 cut(s)	271, 897
MmeI	TCCRAC	4 cut(s)	518, 1054, 2172, 2303
Mph1103I	ATGCAT	1 cut(s)	469
MroI	TCCGGA	1 cut(s)	2246
MroXI	GAANNNNTTC	3 cut(s)	1184, 1964, 2695
MseI	TTAA	7 cut(s)	998, 1013, 1401, 1485, 1569, 1755, 2682
MspCI	CTTAAG	1 cut(s)	997
MspI	CCGG	5 cut(s)	197, 232, 941, 2247, 2556
MspR9I	CCNGG	7 cut(s)	232, 1366, 1605, 1786, 2313, 2556, 2557
Mva1269I	GAATGC	3 cut(s)	1135, 1818, 2538
MvaI	CCWGG	4 cut(s)	1366, 1605, 1786, 2313
MvnI	CGCG	1 cut(s)	1211
NciI	CCSGG	3 cut(s)	232, 2556, 2557
NcoI	CCATGG	1 cut(s)	1471
NdeII	GATC	6 cut(s)	540, 1443, 1644, 1779, 2008, 2646
NlaIV	GGNNCC	6 cut(s)	43, 294, 600, 2131, 2196, 2672
NmuCI	GTSAC	5 cut(s)	331, 409, 806, 2210, 2370
NsiI	ATGCAT	1 cut(s)	469
NspI	RCATGY	6 cut(s)	471, 635, 948, 1054, 1360, 1714
NspV	TTCGAA	1 cut(s)	641
PaeI	GCATGC	1 cut(s)	471
PaeR7I	CTCGAG	1 cut(s)	775
PagI	TCATGA	1 cut(s)	1590
PaqCI	CACCTGC	1 cut(s)	2096
PceI	AGGCCT	2 cut(s)	1891, 1956
PciI	ACATGT	3 cut(s)	1050, 1356, 1710
PctI	GAATGC	3 cut(s)	1135, 1818, 2538
PdmI	GAANNNNTTC	3 cut(s)	1184, 1964, 2695
PfeI	GAWTC	4 cut(s)	340, 367, 1268, 2250
PflFI	GACNNNGTC	1 cut(s)	1100
PflMI	CCANNNNNTGG	1 cut(s)	862
PkrI	GCNGC	9 cut(s)	155, 529, 669, 1204, 1615, 1730, 1924, 2173, 2391
PleI	GAGTC	2 cut(s)	271, 897
PpsI	GAGTC	2 cut(s)	271, 897
Ppu21I	YACGTR	2 cut(s)	264, 1336
PscI	ACATGT	3 cut(s)	1050, 1356, 1710
Psp6I	CCWGG	4 cut(s)	1364, 1603, 1784, 2311
PspEI	GGTNACC	1 cut(s)	409
PspGI	CCWGG	4 cut(s)	1364, 1603, 1784, 2311
PspN4I	GGNNCC	6 cut(s)	43, 294, 600, 2131, 2196, 2672
PspPI	GGNCC	2 cut(s)	41, 234
PstI	CTGCAG	2 cut(s)	319, 2190
PsuI	RGATCY	1 cut(s)	1644
PsyI	GACNNNGTC	1 cut(s)	1100
RsaI	GTAC	7 cut(s)	266, 773, 1163, 1334, 1361, 1794, 2463
RsaNI	GTAC	7 cut(s)	265, 772, 1162, 1333, 1360, 1793, 2462
SaqAI	TTAA	7 cut(s)	998, 1013, 1401, 1485, 1569, 1755, 2682
SatI	GCNGC	9 cut(s)	154, 528, 668, 1203, 1614, 1729, 1923, 2172, 2390
Sau3AI	GATC	6 cut(s)	540, 1443, 1644, 1779, 2008, 2646
Sau96I	GGNCC	2 cut(s)	41, 234
ScaI	AGTACT	1 cut(s)	773
SchI	GAGTC	2 cut(s)	271, 897
ScrFI	CCNGG	7 cut(s)	232, 1366, 1605, 1786, 2313, 2556, 2557
SduI	GDGCHC	1 cut(s)	1627
SexAI	ACCWGGT	1 cut(s)	2311
SfaNI	GCATC	4 cut(s)	185, 454, 556, 1468
SfcI	CTRYAG	2 cut(s)	315, 2186
Sfr274I	CTCGAG	1 cut(s)	775
SfuI	TTCGAA	1 cut(s)	641
SinI	GGWCC	1 cut(s)	234
SlaI	CTCGAG	1 cut(s)	775
SmaI	CCCGGG	1 cut(s)	2557
SmlI	CTYRAG	2 cut(s)	775, 997
SmoI	CTYRAG	2 cut(s)	775, 997
SnaBI	TACGTA	1 cut(s)	264
SphI	GCATGC	1 cut(s)	471
SseBI	AGGCCT	2 cut(s)	1891, 1956
SsiI	CCGC	9 cut(s)	153, 156, 220, 667, 883, 1111, 1209, 1261, 2703
SspMI	CTAG	3 cut(s)	603, 1743, 2208
StuI	AGGCCT	2 cut(s)	1891, 1956
StyD4I	CCNGG	7 cut(s)	230, 1364, 1603, 1784, 2311, 2554, 2555
StyI	CCWWGG	1 cut(s)	1471
TaaI	ACNGT	7 cut(s)	415, 806, 925, 1185, 1298, 1381, 1750
TaiI	ACGT	5 cut(s)	266, 1338, 1907, 2220, 2664
TatI	WGTACW	4 cut(s)	771, 1359, 1792, 2461
TauI	GCSGC	2 cut(s)	156, 670
TfiI	GAWTC	4 cut(s)	340, 367, 1268, 2250
Tru1I	TTAA	7 cut(s)	998, 1013, 1401, 1485, 1569, 1755, 2682
Tru9I	TTAA	7 cut(s)	998, 1013, 1401, 1485, 1569, 1755, 2682
TscAI	CASTG	4 cut(s)	1012, 1453, 1623, 2614
TseFI	GTSAC	5 cut(s)	331, 409, 806, 2210, 2370
TseI	GCWGC	7 cut(s)	527, 1202, 1613, 1728, 1922, 2171, 2389
Tsp45I	GTSAC	5 cut(s)	331, 409, 806, 2210, 2370
TspGWI	ACGGA	1 cut(s)	2651
TspMI	CCCGGG	1 cut(s)	2555
TspRI	CASTG	4 cut(s)	1012, 1453, 1623, 2614
Tth111I	GACNNNGTC	1 cut(s)	1100
Van91I	CCANNNNNTGG	1 cut(s)	862
Vha464I	CTTAAG	1 cut(s)	997
VpaK11BI	GGWCC	1 cut(s)	234
XceI	RCATGY	6 cut(s)	471, 635, 948, 1054, 1360, 1714
XhoI	CTCGAG	1 cut(s)	775
XmaI	CCCGGG	1 cut(s)	2555
XmiI	GTMKAC	1 cut(s)	2028
XmnI	GAANNNNTTC	3 cut(s)	1184, 1964, 2695
XspI	CTAG	3 cut(s)	603, 1743, 2208
ZrmI	AGTACT	1 cut(s)	773
Zsp2I	ATGCAT	1 cut(s)	469

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.