MD10G1236500.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006392
Physical Location & Seq
Reverse (-)
33302271 .. 33303567
1297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1236500.v1.1.491

Sequence Viewer

Length: 564 bp
ATGTTGGGAGGCCGGTTTTCTAACACCGGCGAGGCATCAACAGAACAGGGGCTTTTACAACAAGCCGGTTACAGTCATTGGCCAAGAAATTCATTTCACTTCAAAGAAAATGTTGTGGATGGTTTTGCGCACTCGAACGAAGACGAAGCAGCAACGGAAAATTGGCAAAGGAAAACAGCCACTGTTTTGTTAAAAAACGGCAACAGGAAACTACTCGTGGCCCTTTCGGATTCCATGGCGAATGGCATCCCGAGTTTAGCGCGATCGAGCCTTGTGACGGTGTCGTGGATGAGTAGGTTTGTCGATGCAGTCGGAGATGAAGATTTAGGGTCCATGGCATGCTCGGTTCTTGTGCCTCAGCTGCTAGAATCACTGAATTATGACAGAGATGTGGAGGAAAGAGTGCTTGCTTCATATACATTGCTGAATCTAGCAAAAAGTTCAGTTCATCAAAACGTCCCTATGCTATCATCAGTGGGCAAAGAGTTGCTTAGGAAACTTCAGAACCTGTCCTTAGTGACATGGACGGCTAATGAGCTTATTTCAATCATCACAAGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

20.57

Weight (kDa)

5.55

Isoelectric Point (pI)

29.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN_C PF23628 1 - 184 1.1e-53 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 129
AccII CGCG 1 cut(s) 262
AcoI YGGCCR 1 cut(s) 80
AcsI RAATTY 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 485
AfiI CCNNNNNNNGG 1 cut(s) 277
AgsI TTSAA 2 cut(s) 103, 546
AluBI AGCT 2 cut(s) 361, 538
AluI AGCT 2 cut(s) 361, 538
AlwNI CAGNNNCTG 2 cut(s) 182, 508
Ama87I CYCGRG 1 cut(s) 250
AoxI GGCC 3 cut(s) 10, 80, 219
ApeKI GCWGC 2 cut(s) 149, 361
ApoI RAATTY 1 cut(s) 88
AspLEI GCGC 2 cut(s) 130, 262
AspS9I GGNCC 2 cut(s) 220, 330
AvaI CYCGRG 1 cut(s) 250
AvaII GGWCC 1 cut(s) 330
BalI TGGCCA 1 cut(s) 82
BauI CACGAG 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 147
BbvCI CCTCAGC 1 cut(s) 357
BbvI GCAGC 2 cut(s) 161, 348
BccI CCATC 1 cut(s) 113
BceAI ACGGC 2 cut(s) 214, 543
BfaI CTAG 2 cut(s) 365, 431
BisI GCNGC 2 cut(s) 150, 362
BlsI GCNGC 2 cut(s) 151, 363
Bme18I GGWCC 1 cut(s) 330
BmeT110I CYCGRG 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 220, 330
BmiI GGNNCC 1 cut(s) 331
BmsI GCATC 3 cut(s) 44, 255, 295
BpiI GAAGAC 1 cut(s) 147
Bpu10I CCTNAGC 2 cut(s) 357, 491
BsaJI CCNNGG 2 cut(s) 234, 333
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse118I RCCGGY 3 cut(s) 12, 26, 65
Bse3DI GCAATG 1 cut(s) 419
BseDI CCNNGG 2 cut(s) 234, 333
BseGI GGATG 3 cut(s) 124, 246, 294
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMI GCAATG 1 cut(s) 419
BseMII CTCAG 1 cut(s) 371
BseXI GCAGC 2 cut(s) 161, 348
Bsh1236I CGCG 1 cut(s) 262
Bsh1285I CGRYCG 1 cut(s) 266
BshFI GGCC 3 cut(s) 12, 82, 221
BsiEI CGRYCG 1 cut(s) 266
BsiHKCI CYCGRG 1 cut(s) 250
BsiSI CCGG 3 cut(s) 13, 27, 66
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 443
BsnI GGCC 3 cut(s) 12, 82, 221
BsoBI CYCGRG 1 cut(s) 250
Bsp143I GATC 1 cut(s) 263
Bsp19I CCATGG 2 cut(s) 234, 333
BspANI GGCC 3 cut(s) 12, 82, 221
BspCNI CTCAG 1 cut(s) 370
BspFNI CGCG 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 419
BsrFI RCCGGY 3 cut(s) 12, 26, 65
BssAI RCCGGY 3 cut(s) 12, 26, 65
BssECI CCNNGG 2 cut(s) 234, 333
BssMI GATC 1 cut(s) 263
BssSI CACGAG 1 cut(s) 215
BssT1I CCWWGG 2 cut(s) 234, 333
Bst2BI CACGAG 1 cut(s) 215
Bst4CI ACNGT 3 cut(s) 74, 184, 280
BstC8I GCNNGC 2 cut(s) 340, 408
BstDEI CTNAG 3 cut(s) 357, 491, 514
BstDSI CCRYGG 2 cut(s) 234, 333
BstF5I GGATG 3 cut(s) 124, 246, 294
BstFNI CGCG 1 cut(s) 262
BstHHI GCGC 2 cut(s) 130, 262
BstKTI GATC 1 cut(s) 266
BstMBI GATC 1 cut(s) 263
BstMCI CGRYCG 1 cut(s) 266
BstMWI GCNNNNNNNGC 1 cut(s) 361
BstNSI RCATGY 1 cut(s) 342
BstUI CGCG 1 cut(s) 262
BstV1I GCAGC 2 cut(s) 161, 348
BstV2I GAAGAC 1 cut(s) 147
BsuRI GGCC 3 cut(s) 12, 82, 221
BtgI CCRYGG 2 cut(s) 234, 333
BtsCI GGATG 3 cut(s) 124, 246, 294
BtsIMutI CAGTG 3 cut(s) 180, 371, 480
Cac8I GCNNGC 2 cut(s) 340, 408
CaiI CAGNNNCTG 2 cut(s) 182, 508
CfoI GCGC 2 cut(s) 130, 262
Cfr10I RCCGGY 3 cut(s) 12, 26, 65
Cfr13I GGNCC 2 cut(s) 220, 330
CviAII CATG 4 cut(s) 235, 334, 339, 522
DdeI CTNAG 3 cut(s) 357, 491, 514
DpnI GATC 1 cut(s) 265
DpnII GATC 1 cut(s) 263
EaeI YGGCCR 1 cut(s) 80
Eco130I CCWWGG 2 cut(s) 234, 333
Eco47I GGWCC 1 cut(s) 330
Eco57I CTGAAG 1 cut(s) 485
Eco88I CYCGRG 1 cut(s) 250
EcoT14I CCWWGG 2 cut(s) 234, 333
ErhI CCWWGG 2 cut(s) 234, 333
FaeI CATG 4 cut(s) 238, 337, 342, 525
FaiI YATR 8 cut(s) 236, 335, 340, 381, 415, 417, 464, 523
FalI AAGNNNNNCTT 2 cut(s) 474, 506
FaqI GGGAC 1 cut(s) 443
FatI CATG 4 cut(s) 234, 333, 338, 521
Fnu4HI GCNGC 2 cut(s) 150, 362
FokI GGATG 3 cut(s) 131, 233, 301
Fsp4HI GCNGC 2 cut(s) 150, 362
FspBI CTAG 2 cut(s) 365, 431
FspI TGCGCA 1 cut(s) 129
GlaI GCGC 2 cut(s) 129, 261
GluI GCNGC 2 cut(s) 150, 362
HaeIII GGCC 3 cut(s) 12, 82, 221
HapII CCGG 3 cut(s) 13, 27, 66
HhaI GCGC 2 cut(s) 130, 262
Hin1II CATG 4 cut(s) 238, 337, 342, 525
Hin6I GCGC 2 cut(s) 128, 260
HinP1I GCGC 2 cut(s) 128, 260
HinfI GANTC 3 cut(s) 230, 368, 427
HpaII CCGG 3 cut(s) 13, 27, 66
Hpy188I TCNGA 3 cut(s) 229, 314, 504
Hpy188III TCNNGA 1 cut(s) 250
HpyCH4III ACNGT 3 cut(s) 74, 184, 280
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 1 cut(s) 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 361
HpyF3I CTNAG 3 cut(s) 357, 491, 514
HpySE526I ACGT 1 cut(s) 456
Hsp92II CATG 4 cut(s) 238, 337, 342, 525
HspAI GCGC 2 cut(s) 128, 260
Kzo9I GATC 1 cut(s) 263
LpnPI CCDG 6 cut(s) 26, 32, 40, 79, 190, 521
Lsp1109I GCAGC 2 cut(s) 161, 348
LweI GCATC 3 cut(s) 44, 255, 295
MaeI CTAG 2 cut(s) 365, 431
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 3 cut(s) 68, 274, 517
MalI GATC 1 cut(s) 265
MboI GATC 1 cut(s) 263
MboII GAAGA 2 cut(s) 152, 332
MlsI TGGCCA 1 cut(s) 82
MluCI AATT 3 cut(s) 88, 160, 376
MluNI TGGCCA 1 cut(s) 82
MmeI TCCRAC 1 cut(s) 292
MnlI CCTC 3 cut(s) 25, 366, 388
Mox20I TGGCCA 1 cut(s) 82
MscI TGGCCA 1 cut(s) 82
MseI TTAA 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 82
MspA1I CMGCKG 1 cut(s) 361
MspI CCGG 3 cut(s) 13, 27, 66
MvnI CGCG 1 cut(s) 262
MwoI GCNNNNNNNGC 1 cut(s) 361
NcoI CCATGG 2 cut(s) 234, 333
NdeII GATC 1 cut(s) 263
NlaIII CATG 4 cut(s) 238, 337, 342, 525
NlaIV GGNNCC 1 cut(s) 331
NmuCI GTSAC 2 cut(s) 274, 517
NsbI TGCGCA 1 cut(s) 129
NspI RCATGY 1 cut(s) 342
PaeI GCATGC 1 cut(s) 342
PfeI GAWTC 3 cut(s) 230, 368, 427
PflFI GACNNNGTC 1 cut(s) 280
PkrI GCNGC 2 cut(s) 151, 363
Ple19I CGATCG 1 cut(s) 266
PspN4I GGNNCC 1 cut(s) 331
PspPI GGNCC 2 cut(s) 220, 330
PstNI CAGNNNCTG 2 cut(s) 182, 508
PsyI GACNNNGTC 1 cut(s) 280
PvuI CGATCG 1 cut(s) 266
PvuII CAGCTG 1 cut(s) 361
SaqAI TTAA 1 cut(s) 191
SatI GCNGC 2 cut(s) 150, 362
Sau3AI GATC 1 cut(s) 263
Sau96I GGNCC 2 cut(s) 220, 330
SetI ASST 5 cut(s) 299, 363, 459, 510, 540
SfaNI GCATC 3 cut(s) 44, 255, 295
SgrAI CRCCGGYG 1 cut(s) 26
SinI GGWCC 1 cut(s) 330
SphI GCATGC 1 cut(s) 342
Sse9I AATT 3 cut(s) 88, 160, 376
SspMI CTAG 2 cut(s) 365, 431
StyI CCWWGG 2 cut(s) 234, 333
TaaI ACNGT 3 cut(s) 74, 184, 280
TaiI ACGT 1 cut(s) 459
TaqI TCGA 3 cut(s) 134, 266, 303
TasI AATT 3 cut(s) 88, 160, 376
TfiI GAWTC 3 cut(s) 230, 368, 427
Tru1I TTAA 1 cut(s) 191
Tru9I TTAA 1 cut(s) 191
TscAI CASTG 3 cut(s) 187, 378, 480
TseFI GTSAC 2 cut(s) 274, 517
TseI GCWGC 2 cut(s) 149, 361
Tsp45I GTSAC 2 cut(s) 274, 517
TspDTI ATGAA 4 cut(s) 81, 333, 402, 437
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 3 cut(s) 187, 378, 480
Tth111I GACNNNGTC 1 cut(s) 280
VpaK11BI GGWCC 1 cut(s) 330
XapI RAATTY 1 cut(s) 88
XceI RCATGY 1 cut(s) 342
XspI CTAG 2 cut(s) 365, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.