Rroxscaffold_1G00058520

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006392
Physical Location & Seq
Reverse (-)
80423810 .. 80435294
11485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058520.1

Sequence Viewer

Length: 2784 bp
ATGGCTGCTTCTTCCTTACGAGAGTTGCTTACAGAAGAAGCATATCAACGCGGGAACAACAACAACAACATGTTCGTCGCCAAAACCAAAAAACCAGTGAAGTACAGAGTTGCTCCAGATGAGTCTCTGGCTTTGTTGCCTATACACATCTGCCACGATCGAAAAAGCTACGACTTCTCCAAGCACAAGGCTCAAAACTCTGTTATGCGAAAAGGGTTGTCTAGAAGAGTGAGTTCTACTTCAGAGAGGTCGAATTCCAAGACGGTGGTCTCGGAAAGTAGTTCGAGAAGAACAGAACCTACGGCGCTTGATGAAGTTGCCACTAAAGCTGTGGTTTCCATTCTCAGCGGATATGCGGGTCGTTATATAAAGGACGAGGAGTTTAGAGAAGGGATTGAGGAGAAGTGTAAGGCTTGTTTGGCGAGGAAGAAAAGAGATTCGGCTAGTGGAGTACTTGAGACTTTGGAATCAGGTGTTGAAAATGTTAACAAGTTGGTGGAGAATCCAGTTTTTTCGACGAAAGCAATGAGAAAATGTATTGGGAATTTGAGCAGTGCTGTTGCTTCTCTGGATGCCAATAAGTCCAAGATGAATGCTTCGACTTGTGGTATACCCAATTCAAATCTCTCTGCTTGTGCTCAGCTCTACTTGGCAATTGTTCACAAGCTTGAGAGAAATGACTTGGTTTCTGCTAAGCATTTGCTTCAAGTTTTCTGTGATTCACCATTTTTAGCAAGAACCCATTTGCTTCCTGATCTATGGGAACATTTCTTACTTCCACATCTTCTTCATCTGAAGATATGGTATACCAAGGAGATTGAGGTTGTTTCACACTCATTTGAGAAGGAGAAGAGAATGAAATCCTTAACCAAAGTCTATAATGACCAAATGGATTTGGGGACTACAAAGTTTGCTCAATACTACAAAGAGTGGCTTAAATTTGGGGCTGAGGCTCCTCCTGTTGCTCCTGAAATACCTCTGCCTTCAGTACCAAGTAGCAGATCAAGACGAAGGAGATCATCCGATTCTTTCGCTTCACATTCCTCATTGAACAAAAATTTATATCAGGCTGTGTTTGGCCCCACACTCGAAAGGCGATCAGTGGACCTTGATGATCGAAATGGAGTTTCAATTGCTTCTTGGGATGTAAATGAAGAGGAGAAGCTGTTTGAAGATGAAGCTAAGGCAGACAAGTACAACAGTCTCAGTTTTGTTCATAGGGAAGACAGTAATAGACGGAAGTCATCGAGCCGAAGTCATAGAAATCCAAAAACTGAGTTATGGCCTGAGTCTGATCAGACAAAGAAATCAGACTATTTTGGATTCTTTAGCTGCCAGAATGCACCTACTGAATGCCTAGTGAATAGCAATCTCATATTTAAGAGTAATTCAGTTCAACAAGAAGACAATTCCCACCTTCCTTCAAGCAGTTTAGGCAGTGCAATCAGTACTCTTTACTCGTCGGATAGTCTAAGTGATTGCGAAATTGCAGTTCGTGCAATTACCAAAGCTTGGTTGGACTCACATGGTGATCCTGTCATTGAAGCCATGTTATCCGAGCCACCTCTTATCCAGGGAATGCTGGAGGTGTTGTTTGCTTCCACTGATGATGAAATTTTGGAACTAGTAATATCGGTTTTAGCTGAATTTGTAGCAAGAAATGAGCAGAATAGAAAGATAATACTGAATTTTGATCCACAGCTTGAAATCTTCACGAGACTTCTAAGAAGTAGTGGTCTATTTTTGAAAGCAGCTGTTTTGCTTTACCTGTTGATGCCAAAGGCAAAACAGATGATATCACTTGAATGGGTGGCACTAGTCCTTCGAGTACTCGAGTTTGGAGACCAGTTGCAGACCCTATTCACCTTACGATGCAGCCCTCAAGCAGCAGCGTTGTACCTTTTGGACCAGCTTCTTACGGGTTTTGATGAAGATCGGAACTTGGAGAATGCTAGACAGGTGGTTTCTCTAGGAGGCTTAAGCCTTCTGGTGACTCAAATTGAGAAAGGGGATGCTCATGAGAGGAATAATGTTGCTTCCATCATTTCATGCTGTGTTCGAGCTGATGGAAGCTGTCGAAACTACTTGGCTGATTTTCTGAACAAGCCTTCTCTTCTCGAACTCATTGTACTCGGAAATGGAAGCAACTCAACTTGTTCTGCATTTGCTCTATTGATTGAAATACTCTGCCTTAGTAGACGAACGAAGATCACTCAAATCCTAGAAGGACTGAAAGAAGGATGCTGTGGCTTGAACACAATGCAAATTTTATTGGTCTATCTGCAGAGAGCTTCACCAGGAGAGCGCCCCTTAGTTGCAGCCATATTGCTACAACTTGATCTTATGAATGAAGACGAAGAAGCAACAGAAAATTGGCAGCGGAAAGCAGCCACTGCATTGTTTAGAAGCGGTGGTAAGAAATTACTGGGGGCCCTTTCGGATTCCATAGCAAATGGCATCCTGAGTTTAGCACGAGTGAGCCTTGTGACATTGTCCTGGATGAGTAGCTTTCTCATTACAGTTGGAGATGAACATTTGAAGTCCATGGCATGCTCAATTCTTGTGCCTCGGTTGCTCGAGTCGCTGAAGTTTCACAAAGATGTGGAGGAAAGAGTACTTGCTTCTTACTCATTACTGAATCTTGTAAAAAGTTCAGGTGATGAATACATCCCCATGCTCTCATCAGTAGACAAAGAGGTTCTTAGCAAACTCCAGAACCTCTCCTTAGTGACATGGACAGCTAATGAGCTTATTTCAATCGTCGCAAGCAATTATGAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

927

Amino Acids

103.41

Weight (kDa)

6.57

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 106 - 329 1.4e-90 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_2nd PF23654 475 - 636 4.3e-59 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 637 - 784 7.6e-53 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
ARM_LIN_C PF23628 783 - 921 1.6e-41 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1512
AccI GTMKAC 4 cut(s) 610, 806, 2197, 2688
AccII CGCG 1 cut(s) 51
AciI CCGC 5 cut(s) 51, 348, 356, 2380, 2409
AclWI GGATC 2 cut(s) 1526, 1688
AcsI RAATTY 8 cut(s) 253, 544, 938, 1057, 1614, 1646, 1687, 2265
AcuI CTGAAG 4 cut(s) 225, 815, 969, 2606
AdeI CACNNNGTG 1 cut(s) 1529
AfaI GTAC 9 cut(s) 104, 453, 990, 1196, 1450, 1830, 1898, 2130, 2616
AfiI CCNNNNNNNGG 1 cut(s) 1512
AflII CTTAAG 1 cut(s) 1978
AflIII ACRYGT 1 cut(s) 69
AhlI ACTAGT 2 cut(s) 1624, 1816
AjnI CCWGG 3 cut(s) 1572, 2296, 2495
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 6 cut(s) 129, 274, 452, 1208, 1711, 1836
AlwI GGATC 2 cut(s) 1526, 1688
AlwNI CAGNNNCTG 1 cut(s) 2393
Ama87I CYCGRG 2 cut(s) 1832, 2576
AoxI GGCC 3 cut(s) 1078, 1283, 2430
ApaI GGGCCC 1 cut(s) 2434
ApeKI GCWGC 9 cut(s) 5, 1332, 1751, 1875, 1886, 1889, 2318, 2377, 2387
ApoI RAATTY 8 cut(s) 253, 544, 938, 1057, 1614, 1646, 1687, 2265
AspLEI GCGC 2 cut(s) 307, 2307
AspS9I GGNCC 5 cut(s) 1079, 1105, 1906, 2430, 2431
AsuHPI GGTGA 6 cut(s) 714, 1541, 1855, 2002, 2286, 2669
AvaI CYCGRG 2 cut(s) 1832, 2576
AvaII GGWCC 2 cut(s) 1105, 1906
BaeGI GKGCMC 1 cut(s) 2434
BanII GRGCYC 1 cut(s) 2434
BauI CACGAG 2 cut(s) 1714, 2472
BbsI GAAGAC 3 cut(s) 1230, 1410, 2358
Bbv12I GWGCWC 1 cut(s) 640
BbvCI CCTCAGC 1 cut(s) 948
BbvI GCAGC 8 cut(s) 1319, 1763, 1887, 1898, 1901, 2330, 2389, 2399
BccI CCATC 2 cut(s) 2048, 2060
BceAI ACGGC 1 cut(s) 318
BciT130I CCWGG 3 cut(s) 1574, 2298, 2497
BclI TGATCA 1 cut(s) 1294
BcoDI GTCTC 6 cut(s) 129, 274, 452, 1208, 1711, 1836
BcuI ACTAGT 2 cut(s) 1624, 1816
BfaI CTAG 9 cut(s) 222, 444, 1358, 1625, 1817, 1953, 1970, 2222, 2782
BfmI CTRYAG 1 cut(s) 2282
BfoI RGCGCY 2 cut(s) 308, 2308
BfrI CTTAAG 1 cut(s) 1978
BisI GCNGC 9 cut(s) 6, 1333, 1752, 1876, 1887, 1890, 2319, 2378, 2388
BlpI GCTNAGC 2 cut(s) 639, 693
BlsI GCNGC 9 cut(s) 7, 1334, 1753, 1877, 1888, 1891, 2320, 2379, 2389
BmcAI AGTACT 4 cut(s) 453, 1450, 1830, 2616
Bme1390I CCNGG 3 cut(s) 1574, 2298, 2497
Bme18I GGWCC 2 cut(s) 1105, 1906
BmeT110I CYCGRG 2 cut(s) 1832, 2576
BmgT120I GGNCC 5 cut(s) 1079, 1105, 1906, 2430, 2431
BmiI GGNNCC 4 cut(s) 954, 1081, 2431, 2432
BmrFI CCNGG 3 cut(s) 1574, 2298, 2497
BmrI ACTGGG 1 cut(s) 2435
BmsI GCATC 6 cut(s) 562, 1764, 1862, 2002, 2231, 2466
BmuI ACTGGG 1 cut(s) 2435
BoxI GACNNNNGTC 2 cut(s) 266, 1240
BpiI GAAGAC 3 cut(s) 1230, 1410, 2358
BpmI CTGGAG 3 cut(s) 99, 1604, 2696
Bpu10I CCTNAGC 2 cut(s) 948, 1182
Bpu1102I GCTNAGC 2 cut(s) 639, 693
BpuEI CTTGAG 3 cut(s) 476, 689, 1866
BsaBI GATNNNNATC 1 cut(s) 1932
BsaI GGTCTC 2 cut(s) 274, 1836
BsaJI CCNNGG 4 cut(s) 810, 1573, 2544, 2567
BsaXI ACNNNNNCTCC 6 cut(s) 161, 191, 1151, 1181, 1577, 1607
Bsc4I CCNNNNNNNGG 1 cut(s) 1512
Bse1I ACTGG 4 cut(s) 95, 506, 1846, 2430
Bse3DI GCAATG 1 cut(s) 531
Bse8I GATNNNNATC 1 cut(s) 1932
BseBI CCWGG 3 cut(s) 1574, 2298, 2497
BseDI CCNNGG 4 cut(s) 810, 1573, 2544, 2567
BseGI GGATG 8 cut(s) 577, 1019, 1150, 2017, 2246, 2457, 2505, 2667
BseJI GATNNNNATC 1 cut(s) 1932
BseLI CCNNNNNNNGG 1 cut(s) 1512
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 7 cut(s) 358, 653, 939, 1219, 1266, 1278, 2453
BseNI ACTGG 4 cut(s) 95, 506, 1846, 2430
BseRI GAGGAG 4 cut(s) 392, 413, 945, 1172
BseSI GKGCMC 1 cut(s) 2434
BseXI GCAGC 8 cut(s) 1319, 1763, 1887, 1898, 1901, 2330, 2389, 2399
Bsh1236I CGCG 1 cut(s) 51
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 3 cut(s) 1080, 1285, 2432
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 640
BsiHKCI CYCGRG 2 cut(s) 1832, 2576
BslFI GGGAC 1 cut(s) 913
BslI CCNNNNNNNGG 1 cut(s) 1512
BsmAI GTCTC 6 cut(s) 129, 274, 452, 1208, 1711, 1836
BsmFI GGGAC 1 cut(s) 913
BsmI GAATGC 5 cut(s) 598, 1345, 1358, 1584, 1954
BsnI GGCC 3 cut(s) 1080, 1285, 2432
Bso31I GGTCTC 2 cut(s) 274, 1836
BsoBI CYCGRG 2 cut(s) 1832, 2576
Bsp120I GGGCCC 1 cut(s) 2430
Bsp1286I GDGCHC 2 cut(s) 640, 2434
Bsp1720I GCTNAGC 2 cut(s) 639, 693
Bsp19I CCATGG 1 cut(s) 2544
BspACI CCGC 5 cut(s) 51, 348, 356, 2380, 2409
BspANI GGCC 3 cut(s) 1080, 1285, 2432
BspCNI CTCAG 7 cut(s) 357, 652, 940, 1218, 1267, 1279, 2454
BspFNI CGCG 1 cut(s) 51
BspHI TCATGA 1 cut(s) 2017
BspLI GGNNCC 4 cut(s) 954, 1081, 2431, 2432
BspMAI CTGCAG 1 cut(s) 2286
BspPI GGATC 2 cut(s) 1526, 1688
BspTI CTTAAG 1 cut(s) 1978
BspTNI GGTCTC 2 cut(s) 274, 1836
BsrDI GCAATG 1 cut(s) 531
BsrI ACTGG 4 cut(s) 95, 506, 1846, 2430
BssECI CCNNGG 4 cut(s) 810, 1573, 2544, 2567
BssNAI GTATAC 2 cut(s) 611, 807
BssSI CACGAG 2 cut(s) 1714, 2472
BssT1I CCWWGG 2 cut(s) 810, 2544
Bst1107I GTATAC 2 cut(s) 611, 807
Bst2BI CACGAG 2 cut(s) 1714, 2472
Bst2UI CCWGG 3 cut(s) 1574, 2298, 2497
Bst4CI ACNGT 4 cut(s) 265, 1202, 1229, 2521
Bst6I CTCTTC 4 cut(s) 220, 845, 1149, 2118
BstAFI CTTAAG 1 cut(s) 1978
BstAPI GCANNNNNTGC 2 cut(s) 1496, 2393
BstC8I GCNNGC 2 cut(s) 2551, 2767
BstDSI CCRYGG 1 cut(s) 2544
BstF5I GGATG 8 cut(s) 577, 1019, 1150, 2017, 2246, 2457, 2505, 2667
BstFNI CGCG 1 cut(s) 51
BstH2I RGCGCY 2 cut(s) 308, 2308
BstHHI GCGC 2 cut(s) 307, 2307
BstMAI GTCTC 6 cut(s) 129, 274, 452, 1208, 1711, 1836
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 7 cut(s) 326, 419, 1434, 1496, 2393, 2572, 2581
BstNI CCWGG 3 cut(s) 1574, 2298, 2497
BstNSI RCATGY 2 cut(s) 73, 2553
BstPAI GACNNNNGTC 2 cut(s) 266, 1240
BstSCI CCNGG 3 cut(s) 1572, 2296, 2495
BstSFI CTRYAG 1 cut(s) 2282
BstSLI GKGCMC 1 cut(s) 2434
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 8 cut(s) 1319, 1763, 1887, 1898, 1901, 2330, 2389, 2399
BstV2I GAAGAC 3 cut(s) 1230, 1410, 2358
BstXI CCANNNNNNTGG 1 cut(s) 265
BstZ17I GTATAC 2 cut(s) 611, 807
BsuRI GGCC 3 cut(s) 1080, 1285, 2432
BtgI CCRYGG 1 cut(s) 2544
BtsCI GGATG 8 cut(s) 577, 1019, 1150, 2017, 2246, 2457, 2505, 2667
BtsI GCAGTG 3 cut(s) 559, 1444, 2391
BtsIMutI CAGTG 6 cut(s) 102, 559, 1107, 1444, 1602, 2391
Cac8I GCNNGC 2 cut(s) 2551, 2767
CaiI CAGNNNCTG 1 cut(s) 2393
CciI TCATGA 1 cut(s) 2017
CfoI GCGC 2 cut(s) 307, 2307
Cfr13I GGNCC 5 cut(s) 1079, 1105, 1906, 2430, 2431
Csp6I GTAC 9 cut(s) 103, 452, 989, 1195, 1449, 1829, 1897, 2129, 2615
CviAII CATG 9 cut(s) 70, 1526, 1549, 2018, 2049, 2545, 2550, 2674, 2733
CviQI GTAC 9 cut(s) 103, 452, 989, 1195, 1449, 1829, 1897, 2129, 2615
DraIII CACNNNGTG 1 cut(s) 1529
Eam1104I CTCTTC 4 cut(s) 220, 845, 1149, 2118
EarI CTCTTC 4 cut(s) 220, 845, 1149, 2118
Eco130I CCWWGG 2 cut(s) 810, 2544
Eco24I GRGCYC 1 cut(s) 2434
Eco31I GGTCTC 2 cut(s) 274, 1836
Eco32I GATATC 1 cut(s) 1797
Eco47I GGWCC 2 cut(s) 1105, 1906
Eco57I CTGAAG 4 cut(s) 225, 815, 969, 2606
Eco88I CYCGRG 2 cut(s) 1832, 2576
EcoO109I RGGNCCY 2 cut(s) 2430, 2431
EcoRI GAATTC 1 cut(s) 253
EcoRII CCWGG 3 cut(s) 1572, 2296, 2495
EcoRV GATATC 1 cut(s) 1797
EcoT14I CCWWGG 2 cut(s) 810, 2544
EcoT38I GRGCYC 1 cut(s) 2434
ErhI CCWWGG 2 cut(s) 810, 2544
FaeI CATG 9 cut(s) 73, 1529, 1552, 2021, 2052, 2548, 2553, 2677, 2736
FalI AAGNNNNNCTT 4 cut(s) 918, 950, 2685, 2717
FaqI GGGAC 1 cut(s) 913
FatI CATG 9 cut(s) 69, 1525, 1548, 2017, 2048, 2544, 2549, 2673, 2732
FauI CCCGC 2 cut(s) 44, 349
FbaI TGATCA 1 cut(s) 1294
FblI GTMKAC 4 cut(s) 610, 806, 2197, 2688
Fnu4HI GCNGC 9 cut(s) 6, 1333, 1752, 1876, 1887, 1890, 2319, 2378, 2388
FokI GGATG 8 cut(s) 584, 1006, 1157, 2024, 2253, 2444, 2512, 2654
FriOI GRGCYC 1 cut(s) 2434
Fsp4HI GCNGC 9 cut(s) 6, 1333, 1752, 1876, 1887, 1890, 2319, 2378, 2388
FspBI CTAG 9 cut(s) 222, 444, 1358, 1625, 1817, 1953, 1970, 2222, 2782
GlaI GCGC 2 cut(s) 306, 2306
GluI GCNGC 9 cut(s) 6, 1333, 1752, 1876, 1887, 1890, 2319, 2378, 2388
GsuI CTGGAG 3 cut(s) 99, 1604, 2696
HaeII RGCGCY 2 cut(s) 308, 2308
HaeIII GGCC 3 cut(s) 1080, 1285, 2432
HhaI GCGC 2 cut(s) 307, 2307
Hin1II CATG 9 cut(s) 73, 1529, 1552, 2021, 2052, 2548, 2553, 2677, 2736
Hin6I GCGC 2 cut(s) 305, 2305
HinP1I GCGC 2 cut(s) 305, 2305
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HindIII AAGCTT 2 cut(s) 665, 1509
HpaI GTTAAC 1 cut(s) 487
HphI GGTGA 6 cut(s) 714, 1541, 1855, 2002, 2286, 2669
Hpy166II GTNNAC 7 cut(s) 487, 611, 661, 807, 1105, 2198, 2689
Hpy8I GTNNAC 7 cut(s) 487, 611, 661, 807, 1105, 2198, 2689
Hpy99I CGWCG 4 cut(s) 80, 520, 1465, 2765
HpyCH4III ACNGT 4 cut(s) 265, 1202, 1229, 2521
HpyF10VI GCNNNNNNNGC 7 cut(s) 326, 419, 1434, 1496, 2393, 2572, 2581
Hsp92II CATG 9 cut(s) 73, 1529, 1552, 2021, 2052, 2548, 2553, 2677, 2736
HspAI GCGC 2 cut(s) 305, 2305
Ksp22I TGATCA 1 cut(s) 1294
KspAI GTTAAC 1 cut(s) 487
LmnI GCTCC 3 cut(s) 118, 958, 970
Lsp1109I GCAGC 8 cut(s) 1319, 1763, 1887, 1898, 1901, 2330, 2389, 2399
LweI GCATC 6 cut(s) 562, 1764, 1862, 2002, 2231, 2466
MaeI CTAG 9 cut(s) 222, 444, 1358, 1625, 1817, 1953, 1970, 2222, 2782
MaeIII GTNAC 3 cut(s) 1990, 2485, 2728
MfeI CAATTG 2 cut(s) 654, 1131
MhlI GDGCHC 2 cut(s) 640, 2434
MlyI GAGTC 5 cut(s) 131, 1298, 1514, 1987, 2588
MmeI TCCRAC 3 cut(s) 1443, 1497, 2503
MseI TTAA 5 cut(s) 486, 866, 936, 1380, 1979
MslI CAYNNNNRTG 3 cut(s) 1804, 2598, 2672
MspA1I CMGCKG 3 cut(s) 348, 1754, 2380
MspCI CTTAAG 1 cut(s) 1978
MspR9I CCNGG 3 cut(s) 1574, 2298, 2497
MunI CAATTG 2 cut(s) 654, 1131
Mva1269I GAATGC 5 cut(s) 598, 1345, 1358, 1584, 1954
MvaI CCWGG 3 cut(s) 1574, 2298, 2497
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 7 cut(s) 326, 419, 1434, 1496, 2393, 2572, 2581
NcoI CCATGG 1 cut(s) 2544
NlaIII CATG 9 cut(s) 73, 1529, 1552, 2021, 2052, 2548, 2553, 2677, 2736
NlaIV GGNNCC 4 cut(s) 954, 1081, 2431, 2432
NmuCI GTSAC 3 cut(s) 1990, 2485, 2728
NspI RCATGY 2 cut(s) 73, 2553
PaeI GCATGC 1 cut(s) 2553
PaeR7I CTCGAG 2 cut(s) 1832, 2576
PagI TCATGA 1 cut(s) 2017
PciI ACATGT 1 cut(s) 69
PctI GAATGC 5 cut(s) 598, 1345, 1358, 1584, 1954
PfeI GAWTC 8 cut(s) 437, 467, 502, 719, 1025, 1323, 2441, 2638
PflFI GACNNNGTC 1 cut(s) 2491
PflMI CCANNNNNTGG 1 cut(s) 1512
PfoI TCCNGGA 1 cut(s) 2495
PkrI GCNGC 9 cut(s) 7, 1334, 1753, 1877, 1888, 1891, 2320, 2379, 2389
Ple19I CGATCG 1 cut(s) 160
PleI GAGTC 5 cut(s) 130, 1297, 1514, 1987, 2587
PpsI GAGTC 5 cut(s) 130, 1297, 1514, 1987, 2587
PscI ACATGT 1 cut(s) 69
PshAI GACNNNNGTC 2 cut(s) 266, 1240
Psp6I CCWGG 3 cut(s) 1572, 2296, 2495
PspGI CCWGG 3 cut(s) 1572, 2296, 2495
PspN4I GGNNCC 4 cut(s) 954, 1081, 2431, 2432
PspOMI GGGCCC 1 cut(s) 2430
PspPI GGNCC 5 cut(s) 1079, 1105, 1906, 2430, 2431
PspXI VCTCGAGB 2 cut(s) 1832, 2576
PsrI GAACNNNNNNTAC 6 cut(s) 283, 315, 756, 788, 2112, 2144
PstI CTGCAG 1 cut(s) 2286
PstNI CAGNNNCTG 1 cut(s) 2393
PsyI GACNNNGTC 1 cut(s) 2491
PvuI CGATCG 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 1754
RsaI GTAC 9 cut(s) 104, 453, 990, 1196, 1450, 1830, 1898, 2130, 2616
RsaNI GTAC 9 cut(s) 103, 452, 989, 1195, 1449, 1829, 1897, 2129, 2615
RseI CAYNNNNRTG 3 cut(s) 1804, 2598, 2672
SaqAI TTAA 5 cut(s) 486, 866, 936, 1380, 1979
SatI GCNGC 9 cut(s) 6, 1333, 1752, 1876, 1887, 1890, 2319, 2378, 2388
Sau96I GGNCC 5 cut(s) 1079, 1105, 1906, 2430, 2431
ScaI AGTACT 4 cut(s) 453, 1450, 1830, 2616
SchI GAGTC 5 cut(s) 131, 1298, 1514, 1987, 2588
ScrFI CCNGG 3 cut(s) 1574, 2298, 2497
SduI GDGCHC 2 cut(s) 640, 2434
SfaNI GCATC 6 cut(s) 562, 1764, 1862, 2002, 2231, 2466
SfcI CTRYAG 1 cut(s) 2282
Sfr274I CTCGAG 2 cut(s) 1832, 2576
SinI GGWCC 2 cut(s) 1105, 1906
SlaI CTCGAG 2 cut(s) 1832, 2576
SmiMI CAYNNNNRTG 3 cut(s) 1804, 2598, 2672
SmlI CTYRAG 6 cut(s) 455, 668, 1832, 1881, 1978, 2576
SmoI CTYRAG 6 cut(s) 455, 668, 1832, 1881, 1978, 2576
SpeI ACTAGT 2 cut(s) 1624, 1816
SphI GCATGC 1 cut(s) 2553
SsiI CCGC 5 cut(s) 51, 348, 356, 2380, 2409
SspMI CTAG 9 cut(s) 222, 444, 1358, 1625, 1817, 1953, 1970, 2222, 2782
StyD4I CCNGG 3 cut(s) 1572, 2296, 2495
StyI CCWWGG 2 cut(s) 810, 2544
TaaI ACNGT 4 cut(s) 265, 1202, 1229, 2521
TatI WGTACW 7 cut(s) 102, 451, 1194, 1448, 1828, 2128, 2614
TfiI GAWTC 8 cut(s) 437, 467, 502, 719, 1025, 1323, 2441, 2638
Tru1I TTAA 5 cut(s) 486, 866, 936, 1380, 1979
Tru9I TTAA 5 cut(s) 486, 866, 936, 1380, 1979
TscAI CASTG 6 cut(s) 102, 559, 1107, 1444, 1609, 2398
TseFI GTSAC 3 cut(s) 1990, 2485, 2728
TseI GCWGC 9 cut(s) 5, 1332, 1751, 1875, 1886, 1889, 2318, 2377, 2387
Tsp45I GTSAC 3 cut(s) 1990, 2485, 2728
TspGWI ACGGA 1 cut(s) 1252
TspRI CASTG 6 cut(s) 102, 559, 1107, 1444, 1609, 2398
Tth111I GACNNNGTC 1 cut(s) 2491
Van91I CCANNNNNTGG 1 cut(s) 1512
Vha464I CTTAAG 1 cut(s) 1978
VpaK11BI GGWCC 2 cut(s) 1105, 1906
XapI RAATTY 8 cut(s) 253, 544, 938, 1057, 1614, 1646, 1687, 2265
XbaI TCTAGA 1 cut(s) 221
XceI RCATGY 2 cut(s) 73, 2553
XcmI CCANNNNNNNNNTGG 2 cut(s) 328, 1513
XhoI CTCGAG 2 cut(s) 1832, 2576
XmiI GTMKAC 4 cut(s) 610, 806, 2197, 2688
XspI CTAG 9 cut(s) 222, 444, 1358, 1625, 1817, 1953, 1970, 2222, 2782
ZrmI AGTACT 4 cut(s) 453, 1450, 1830, 2616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.