Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0006392

Physical Location & Seq

Genomic Coordinates Reverse (-)

25401354 .. 25404968

Gene Structure 3615 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g23530.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2955 bp

ATGGCTTCCTTGAAGGAAATGCTCACTGAAGAAAGACTCGAACTCGAAAGCAAAAGGTACCCGAAAGGCCAGAAACCATTCAAGTACAGAGAAATATATGAATCTATGGCTCTTTTGCTGCCTATACACATCTGCCATGATCGGAAAAGTGGCGATTTTTCGGACCACAAGGCTCCGACGCGAAAAGGGTTGTCGAGAAGGGTTAATACTACTTCGGAGAGGTCGAATTCCAAGTCATTGGTTTCGGAGAGTTCGAGGACTAGTGAGCCTGCTGCCATTGATGAAGTTGCCACTGGAGCTGTGGTTTCTATACTTAGTGGTTACGTAGGGAGGTACGTGAAGGATGAGGCTTTTCAGGAAACAATCCGTGAAAAGTGCAGGTCTTGCTTGGTGAGGAAGAAAAAGGATTTGGATAATGGGATGTTGGGAAACTTGGAATCGGGTATTGAAAGTGTTGAGAAGTTGGTGGAAGATCAATGGTCGACGAAGAAGGAAGTTAGGGCGAAAACAGTGAAGAATTTGATTCGGGTTTTTAACGTTGTTGGTTCCTCAAATTCTAGCAATTCTCATATTTCTGCTTGTGCTCAGCTTTACTTGTCAATTGTTCACAAGCTTGAGAAAAATGACCAGCTTTCGTCATGGCACTTGCTTCAAGTGTTCTGTGATTCACCGGGTTTGGCACGCACACATTTGCTCCCTGATCTTTGGGAGCATTTGTTTCTTCCACATCTTTTACATGTGAAGGTTTGGTATGCCAAGGAAATGGATGCTCTTTCGAGCTCAGAGGACGGTGAGAAGGAGAAGAAAATGAAAGCCATGACGAGCGTGTATGATGAACAAATGGATATGGGAACAAAAAAGTTTGCTTTGTACTACAAGGAGTGGCTTCAAGTGGGGGCTGAGGCTCCTCCCATTCCTCCTACTATACCTTTACCTTCTTCGATAAGCAGTTGCAGATCATCGAGAAGGAGATCATCAGATTCTTACACTTCACATTCCTCACTCAACAAAAATTTATACCGATCTGTGTTTGGCTCCACGCTTGAGCGAAGATCTGTGAACCTCGATGATGGAAAAGGAGTTTTAAATGATACTTGGGGTTTGGATGAAGAGGAGGAAAATCTTTGTGCAGATTATGTTCATAGGGGAGAAAGGTCTAGTTGGCGAAGGTCATCAAGCGAAAGTCATAGAAATCCGATAATTGCGCCATGGCCTGAGTCAGAGACACAGAAGTCAGACCATTTTGGATTCTTTAGATGCCAGAATGCACCAACAGTATGCTTGGTGAGCAAGAATCTCATAGTCAAGAACAATTCAATCCGAAAAGAAGATAACTCCCATCTTCGACCTTCAAGCAACTTAAGCCGTGCTATTAGCACTATCTGCTCCTCCGATAATCCAAGTGATTGCGAAACTGCAATTCGTGTGATTACCAAGGCTTGGTTGGACTCACATGGTGATCCTGTCGTTGAGGCTGAGCTATCAAAGCCACCCGTTATTCAGGGAATGTTGGAGGTGTTGTTTGCTTCTAGTGATGAAGAAATTTTGGAATTAGTAATCTCAGTTTTAGCTGAACTCACAACGAGAAACGAGATGATTAGACAGATCATACTGAACTCAGATCCACAGCTTGAAATTTTTATGAGACTTCTAAGAAGTAATGGTCTATTTTTGAAAGCTGCAATTCTGCTTTACTTGTTGAAGCCAAAGGCAAAACAGATGATATCAGTCGAATGGGCAGCGCTAGTCCTTCGAGTACTAGAGTTTGGAGATCAGTTGCAGATGCTATTCACAGTTCGTTGCAGTCCTCAAGTGGCGGCATTGTACCTTTTAGACCAGCTTCTTACCGGTTTTGATGAAGATCGAAACTTGGAGAATGCTGGAGAGGTAATTTCTCTAGGAGGGCTGAGCCTTCTGGTGGCACAAATCGAGAGAGGGGAGACTCATGAGAGGAACAACATTACTTCGATCATTGTGTGTTGTGTTCGTGCTGATGGTAGGTGTCGAAACTACTTGGCTGATTTTCTCAACAAGGCTTCTCTTCTTGAACTCGTTGTACTTGGAAATGGCAAGAACTCTACTGGCTCCGCATTCACCCTATTGATCGAAATGCTCTGCCTTAGTAGACGAACAAAGATCACTGAAATCCTAGATGGGCTGAAAGAAGGATCGGGCGGCTTTAACACAATGCAGATTTTATTGGTCTATCTGCAGAGAGCTCCACTTGAAGAACGCCCGTTAATTGCATCAGTATTGCTACAGCTTGATCTTATGGGAGACCCTTTCAGGAGCAGTGTATACAGAGAAGAAGCCATTGAAGCAATCATAGAAGCTATAAACTGTCGAACGTGCCACGTAAAAGTCCAGCAACGATCAGCAAGAGCTCTCTCAATGTTGGGAGGCCGGTTTTCTGATGCCGGCGAGGCAACAACAGAACATTGGCTTTTACAAGAAGCAGGTTACAGTCATTGGCCAGGAAATTCATTTCTCTTTAAAGAAAACATTGTGGATGGTTTTGCACACTCAAACGAAGACGAAGCAGCAACGGAAAATTGGCAAAGAAAAACAGCCACTGTTTTGTTCAAAAGTGGCAACAAGGAACTACTCGTGGCCCTTTCGGATTCCATGGCGAATGGCATCCCAAGTTTAGCACGATCGAGCCTTGTGACGGTGTCATGGATGAGTAGATTTCTCGATGCAGTCGGAGGTGAAGATTTGAGGTCAATGACATGGTCAGTTCTTGTGCCTCAGCTGCTAGAATATCTGAATTATGACAGAGATGTGGAGGAAAGAGTGCTTGCTTCATACACACTGCTCAAATTAGCAAAAAGTTCAGCTCATGAATATGTCCCCATGCTATCATCAGCGGACAAAGATTTGCTCAGGAAACTTCAGAACCTCTCCTTTGTGACATGGACAGCCAATGAACTTATCTCAATCATCACAAGCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

985

Amino Acids

110.52

Weight (kDa)

6.13

Isoelectric Point (pI)

47.48

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006392)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g11960 FvH4_3g11960 FvH4_3g11960 FvH4_3g11960 FvH4_3g11960
malus_domestica	MD05G1256200.v1.1 MD10G1236500.v1.1 MD10G1236600.v1.1
prunus_persica	Prupe.4G107900_v2.0.a1 Prupe.4G107900_v2.0.a1 Prupe.4G107900_v2.0.a1
pyrus_communis	pycom05g23530 pycom10g19790
rosa_chinensis	RchiOBHm_Chr5g0019541
rosa_laevigata	RLG00000032455
rosa_multiflora	Rmu_co8318911.1_g000001 Rmu_sc0002759.1_g000051 Rmu_sc0002759.1_g000053
rosa_roxburghii	Rroxscaffold_1G00058520
rosa_rugosa	Rorug05G0051700 Rorug05G0051800 Rorug05G0051900
rosa_samantha	Rh5AG142500 Rh5BG140900 Rh5CG153000 Rh5DG141600
rosa_wichuraiana	Rw5G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1231
Acc36I	ACCTGC	2 cut(s)	369, 2447
Acc65I	GGTACC	1 cut(s)	57
AccB1I	GGYRCC	1 cut(s)	57
AccB7I	CCANNNNNTGG	1 cut(s)	1440
AccI	GTMKAC	3 cut(s)	482, 2125, 2298
AccII	CGCG	1 cut(s)	181
AciI	CCGC	4 cut(s)	1817, 2088, 2175, 2867
AclI	AACGTT	1 cut(s)	537
AclWI	GGATC	3 cut(s)	1454, 1616, 2176
AcoI	YGGCCR	1 cut(s)	2471
AcsI	RAATTY	7 cut(s)	226, 517, 553, 1012, 1542, 1635, 2479
AcuI	CTGAAG	2 cut(s)	48, 2876
AdeI	CACNNNGTG	1 cut(s)	1457
AfaI	GTAC	7 cut(s)	59, 86, 335, 872, 1758, 1826, 2058
AfeI	AGCGCT	1 cut(s)	1743
AfiI	CCNNNNNNNGG	3 cut(s)	1440, 1918, 2668
AflII	CTTAAG	1 cut(s)	1360
AflIII	ACRYGT	1 cut(s)	736
AgeI	ACCGGT	1 cut(s)	1847
AhlI	ACTAGT	1 cut(s)	260
AjnI	CCWGG	1 cut(s)	2473
AjuI	GAANNNNNNNTTGG	2 cut(s)	392, 424
Alw21I	GWGCWC	4 cut(s)	586, 782, 2221, 2386
Alw26I	GTCTC	4 cut(s)	1217, 1639, 1934, 2271
AlwI	GGATC	3 cut(s)	1454, 1616, 2176
AlwNI	CAGNNNCTG	1 cut(s)	2573
Aor51HI	AGCGCT	1 cut(s)	1743
AoxI	GGCC	5 cut(s)	67, 1211, 2401, 2471, 2610
ApeKI	GCWGC	6 cut(s)	118, 272, 1679, 1739, 2540, 2752
ApoI	RAATTY	7 cut(s)	226, 517, 553, 1012, 1542, 1635, 2479
ArsI	GACNNNNNNTTYG	2 cut(s)	218, 250
AsiGI	ACCGGT	1 cut(s)	1847
Asp700I	GAANNNNTTC	1 cut(s)	77
Asp718I	GGTACC	1 cut(s)	57
AspLEI	GCGC	2 cut(s)	1207, 1744
AspS9I	GGNCC	2 cut(s)	163, 2611
AsuC2I	CCSGG	1 cut(s)	672
AsuHPI	GGTGA	7 cut(s)	403, 660, 803, 1297, 1469, 2086, 2720
AvaII	GGWCC	1 cut(s)	163
BalI	TGGCCA	1 cut(s)	2473
BanI	GGYRCC	1 cut(s)	57
BanII	GRGCYC	3 cut(s)	782, 2221, 2386
BarI	GAAGNNNNNNTAC	2 cut(s)	190, 222
BauI	CACGAG	1 cut(s)	2606
BbsI	GAAGAC	1 cut(s)	2538
Bbv12I	GWGCWC	4 cut(s)	586, 782, 2221, 2386
BbvCI	CCTCAGC	2 cut(s)	900, 2748
BbvI	GCAGC	6 cut(s)	105, 259, 1666, 1751, 2552, 2739
BccI	CCATC	5 cut(s)	1064, 1347, 1988, 2147, 2504
BceAI	ACGGC	1 cut(s)	1350
BciT130I	CCWGG	1 cut(s)	2475
BcnI	CCSGG	1 cut(s)	672
BcoDI	GTCTC	4 cut(s)	1217, 1639, 1934, 2271
BcuI	ACTAGT	1 cut(s)	260
BfaI	CTAG	9 cut(s)	261, 558, 1158, 1530, 1745, 1760, 1898, 2150, 2756
BfmI	CTRYAG	2 cut(s)	2210, 2258
BfoI	RGCGCY	1 cut(s)	1745
BfrI	CTTAAG	1 cut(s)	1360
BfuAI	ACCTGC	2 cut(s)	369, 2447
BglI	GCCNNNNNGGC	1 cut(s)	2423
BglII	AGATCT	1 cut(s)	1052
BisI	GCNGC	8 cut(s)	119, 273, 1680, 1740, 1818, 2176, 2541, 2753
BlpI	GCTNAGC	3 cut(s)	585, 1476, 1907
BlsI	GCNGC	8 cut(s)	120, 274, 1681, 1741, 1819, 2177, 2542, 2754
BmcAI	AGTACT	1 cut(s)	1758
Bme1390I	CCNGG	2 cut(s)	672, 2475
Bme18I	GGWCC	1 cut(s)	163
BmgT120I	GGNCC	2 cut(s)	163, 2611
BmiI	GGNNCC	6 cut(s)	59, 174, 547, 906, 1036, 2086
BmrFI	CCNGG	2 cut(s)	672, 2475
BmsI	GCATC	7 cut(s)	757, 1247, 1773, 2255, 2404, 2646, 2686
BpiI	GAAGAC	1 cut(s)	2538
BpmI	CTGGAG	2 cut(s)	315, 1902
Bpu10I	CCTNAGC	3 cut(s)	900, 2748, 2882
Bpu1102I	GCTNAGC	3 cut(s)	585, 1476, 1907
BpuEI	CTTGAG	3 cut(s)	635, 1064, 1794
BpuMI	CCSGG	1 cut(s)	672
BsaAI	YACGTR	3 cut(s)	325, 337, 2356
BsaBI	GATNNNNATC	1 cut(s)	1860
BsaI	GGTCTC	1 cut(s)	2271
BsaJI	CCNNGG	4 cut(s)	756, 1208, 1434, 2625
BsaWI	WCCGGW	1 cut(s)	1847
BsaXI	ACNNNNNCTCC	8 cut(s)	288, 318, 678, 701, 708, 731, 1505, 1535
Bsc4I	CCNNNNNNNGG	3 cut(s)	1440, 1918, 2668
Bse118I	RCCGGY	3 cut(s)	1847, 2403, 2417
Bse1I	ACTGG	2 cut(s)	298, 2086
Bse8I	GATNNNNATC	1 cut(s)	1860
BseBI	CCWGG	1 cut(s)	2475
BseDI	CCNNGG	4 cut(s)	756, 1208, 1434, 2625
BseGI	GGATG	7 cut(s)	349, 426, 772, 1111, 2515, 2637, 2685
BseJI	GATNNNNATC	1 cut(s)	1860
BseLI	CCNNNNNNNGG	3 cut(s)	1440, 1918, 2668
BseNI	ACTGG	2 cut(s)	298, 2086
BseRI	GAGGAG	3 cut(s)	897, 1127, 1378
BseXI	GCAGC	6 cut(s)	105, 259, 1666, 1751, 2552, 2739
BsgI	GTGCAG	2 cut(s)	397, 1149
Bsh1236I	CGCG	1 cut(s)	181
Bsh1285I	CGRYCG	1 cut(s)	2657
BshFI	GGCC	5 cut(s)	69, 1213, 2403, 2473, 2612
BshNI	GGYRCC	1 cut(s)	57
BshTI	ACCGGT	1 cut(s)	1847
BsiEI	CGRYCG	1 cut(s)	2657
BsiHKAI	GWGCWC	4 cut(s)	586, 782, 2221, 2386
BsiSI	CCGG	4 cut(s)	671, 1848, 2404, 2418
BslFI	GGGAC	1 cut(s)	2834
BslI	CCNNNNNNNGG	3 cut(s)	1440, 1918, 2668
BsmAI	GTCTC	4 cut(s)	1217, 1639, 1934, 2271
BsmFI	GGGAC	1 cut(s)	2834
BsmI	GAATGC	3 cut(s)	1270, 1882, 2090
BsnI	GGCC	5 cut(s)	69, 1213, 2403, 2473, 2612
Bso31I	GGTCTC	1 cut(s)	2271
Bsp1286I	GDGCHC	4 cut(s)	586, 782, 2221, 2386
Bsp1720I	GCTNAGC	3 cut(s)	585, 1476, 1907
Bsp19I	CCATGG	2 cut(s)	1208, 2625
BspACI	CCGC	4 cut(s)	1817, 2088, 2175, 2867
BspANI	GGCC	5 cut(s)	69, 1213, 2403, 2473, 2612
BspFNI	CGCG	1 cut(s)	181
BspHI	TCATGA	2 cut(s)	1945, 2839
BspLI	GGNNCC	6 cut(s)	59, 174, 547, 906, 1036, 2086
BspMAI	CTGCAG	1 cut(s)	2214
BspMI	ACCTGC	2 cut(s)	369, 2447
BspPI	GGATC	3 cut(s)	1454, 1616, 2176
BspT107I	GGYRCC	1 cut(s)	57
BspTI	CTTAAG	1 cut(s)	1360
BspTNI	GGTCTC	1 cut(s)	2271
BsrFI	RCCGGY	3 cut(s)	1847, 2403, 2417
BsrI	ACTGG	2 cut(s)	298, 2086
BssAI	RCCGGY	3 cut(s)	1847, 2403, 2417
BssECI	CCNNGG	4 cut(s)	756, 1208, 1434, 2625
BssNAI	GTATAC	1 cut(s)	2299
BssSI	CACGAG	1 cut(s)	2606
BssT1I	CCWWGG	4 cut(s)	756, 1208, 1434, 2625
Bst1107I	GTATAC	1 cut(s)	2299
Bst2BI	CACGAG	1 cut(s)	2606
Bst2UI	CCWGG	1 cut(s)	2475
Bst4CI	ACNGT	8 cut(s)	511, 791, 1276, 1795, 2342, 2465, 2575, 2671
Bst6I	CTCTTC	2 cut(s)	1104, 2046
BstAFI	CTTAAG	1 cut(s)	1360
BstAPI	GCANNNNNTGC	2 cut(s)	384, 1383
BstBAI	YACGTR	3 cut(s)	325, 337, 2356
BstC8I	GCNNGC	4 cut(s)	270, 682, 2419, 2799
BstDSI	CCRYGG	2 cut(s)	1208, 2625
BstF5I	GGATG	7 cut(s)	349, 426, 772, 1111, 2515, 2637, 2685
BstFNI	CGCG	1 cut(s)	181
BstH2I	RGCGCY	1 cut(s)	1745
BstHHI	GCGC	2 cut(s)	1207, 1744
BstMAI	GTCTC	4 cut(s)	1217, 1639, 1934, 2271
BstMCI	CGRYCG	1 cut(s)	2657
BstMWI	GCNNNNNNNGC	9 cut(s)	296, 384, 1287, 1362, 1383, 1486, 2318, 2423, 2752
BstNI	CCWGG	1 cut(s)	2475
BstNSI	RCATGY	1 cut(s)	740
BstSCI	CCNGG	2 cut(s)	670, 2473
BstSFI	CTRYAG	2 cut(s)	2210, 2258
BstSNI	TACGTA	1 cut(s)	325
BstUI	CGCG	1 cut(s)	181
BstV1I	GCAGC	6 cut(s)	105, 259, 1666, 1751, 2552, 2739
BstV2I	GAAGAC	1 cut(s)	2538
BstX2I	RGATCY	2 cut(s)	1052, 1621
BstXI	CCANNNNNNTGG	2 cut(s)	238, 763
BstYI	RGATCY	2 cut(s)	1052, 1621
BstZ17I	GTATAC	1 cut(s)	2299
BsuRI	GGCC	5 cut(s)	69, 1213, 2403, 2473, 2612
BtgI	CCRYGG	2 cut(s)	1208, 2625
BtsCI	GGATG	7 cut(s)	349, 426, 772, 1111, 2515, 2637, 2685
BtsI	GCAGTG	2 cut(s)	2299, 2810
BtsIMutI	CAGTG	7 cut(s)	24, 291, 516, 2139, 2299, 2571, 2810
BveI	ACCTGC	2 cut(s)	369, 2447
Cac8I	GCNNGC	4 cut(s)	270, 682, 2419, 2799
CaiI	CAGNNNCTG	1 cut(s)	2573
CciI	TCATGA	2 cut(s)	1945, 2839
CfoI	GCGC	2 cut(s)	1207, 1744
Cfr10I	RCCGGY	3 cut(s)	1847, 2403, 2417
Cfr13I	GGNCC	2 cut(s)	163, 2611
CseI	GACGC	1 cut(s)	187
Csp6I	GTAC	7 cut(s)	58, 85, 334, 871, 1757, 1825, 2057
CspAI	ACCGGT	1 cut(s)	1847
CviQI	GTAC	7 cut(s)	58, 85, 334, 871, 1757, 1825, 2057
DraI	TTTAAA	2 cut(s)	1086, 2494
DraIII	CACNNNGTG	1 cut(s)	1457
DrdI	GACNNNNNNGTC	1 cut(s)	1231
DseDI	GACNNNNNNGTC	1 cut(s)	1231
EaeI	YGGCCR	1 cut(s)	2471
Eam1104I	CTCTTC	2 cut(s)	1104, 2046
EarI	CTCTTC	2 cut(s)	1104, 2046
Ecl136II	GAGCTC	3 cut(s)	780, 2219, 2384
Eco105I	TACGTA	1 cut(s)	325
Eco130I	CCWWGG	4 cut(s)	756, 1208, 1434, 2625
Eco24I	GRGCYC	3 cut(s)	782, 2221, 2386
Eco31I	GGTCTC	1 cut(s)	2271
Eco32I	GATATC	1 cut(s)	1725
Eco47I	GGWCC	1 cut(s)	163
Eco47III	AGCGCT	1 cut(s)	1743
Eco53kI	GAGCTC	3 cut(s)	780, 2219, 2384
Eco57I	CTGAAG	2 cut(s)	48, 2876
EcoICRI	GAGCTC	3 cut(s)	780, 2219, 2384
EcoRI	GAATTC	1 cut(s)	226
EcoRII	CCWGG	1 cut(s)	2473
EcoRV	GATATC	1 cut(s)	1725
EcoT14I	CCWWGG	4 cut(s)	756, 1208, 1434, 2625
EcoT38I	GRGCYC	3 cut(s)	782, 2221, 2386
ErhI	CCWWGG	4 cut(s)	756, 1208, 1434, 2625
FaqI	GGGAC	1 cut(s)	2834
FblI	GTMKAC	3 cut(s)	482, 2125, 2298
Fnu4HI	GCNGC	8 cut(s)	119, 273, 1680, 1740, 1818, 2176, 2541, 2753
FokI	GGATG	7 cut(s)	356, 433, 779, 1118, 2522, 2624, 2692
FriOI	GRGCYC	3 cut(s)	782, 2221, 2386
Fsp4HI	GCNGC	8 cut(s)	119, 273, 1680, 1740, 1818, 2176, 2541, 2753
FspBI	CTAG	9 cut(s)	261, 558, 1158, 1530, 1745, 1760, 1898, 2150, 2756
GlaI	GCGC	2 cut(s)	1206, 1743
GluI	GCNGC	8 cut(s)	119, 273, 1680, 1740, 1818, 2176, 2541, 2753
GsuI	CTGGAG	2 cut(s)	315, 1902
HaeII	RGCGCY	1 cut(s)	1745
HaeIII	GGCC	5 cut(s)	69, 1213, 2403, 2473, 2612
HapII	CCGG	4 cut(s)	671, 1848, 2404, 2418
HgaI	GACGC	1 cut(s)	187
HhaI	GCGC	2 cut(s)	1207, 1744
Hin6I	GCGC	2 cut(s)	1205, 1742
HinP1I	GCGC	2 cut(s)	1205, 1742
HincII	GTYRAC	1 cut(s)	483
HindII	GTYRAC	1 cut(s)	483
HindIII	AAGCTT	1 cut(s)	611
HpaII	CCGG	4 cut(s)	671, 1848, 2404, 2418
HphI	GGTGA	7 cut(s)	403, 660, 803, 1297, 1469, 2086, 2720
Hpy166II	GTNNAC	5 cut(s)	483, 607, 1060, 2126, 2299
Hpy8I	GTNNAC	5 cut(s)	483, 607, 1060, 2126, 2299
Hpy99I	CGWCG	2 cut(s)	181, 487
HpyCH4III	ACNGT	8 cut(s)	511, 791, 1276, 1795, 2342, 2465, 2575, 2671
HpyCH4IV	ACGT	5 cut(s)	324, 336, 537, 2348, 2355
HpyF10VI	GCNNNNNNNGC	9 cut(s)	296, 384, 1287, 1362, 1383, 1486, 2318, 2423, 2752
HpySE526I	ACGT	5 cut(s)	324, 336, 537, 2348, 2355
HspAI	GCGC	2 cut(s)	1205, 1742
KpnI	GGTACC	1 cut(s)	61
KroI	GCCGGC	1 cut(s)	2417
KroNI	GCCGGC	1 cut(s)	2419
Lsp1109I	GCAGC	6 cut(s)	105, 259, 1666, 1751, 2552, 2739
LweI	GCATC	7 cut(s)	757, 1247, 1773, 2255, 2404, 2646, 2686
MaeI	CTAG	9 cut(s)	261, 558, 1158, 1530, 1745, 1760, 1898, 2150, 2756
MaeII	ACGT	5 cut(s)	324, 336, 537, 2348, 2355
MaeIII	GTNAC	4 cut(s)	320, 2459, 2665, 2908
MfeI	CAATTG	1 cut(s)	600
MflI	RGATCY	2 cut(s)	1052, 1621
MhlI	GDGCHC	4 cut(s)	586, 782, 2221, 2386
MlsI	TGGCCA	1 cut(s)	2473
MluNI	TGGCCA	1 cut(s)	2473
MlyI	GAGTC	4 cut(s)	30, 1226, 1442, 1936
MmeI	TCCRAC	4 cut(s)	200, 1425, 1491, 2683
Mox20I	TGGCCA	1 cut(s)	2473
MroNI	GCCGGC	1 cut(s)	2417
MroXI	GAANNNNTTC	1 cut(s)	77
MscI	TGGCCA	1 cut(s)	2473
MseI	TTAA	7 cut(s)	204, 534, 1085, 1361, 2181, 2240, 2493
MslI	CAYNNNNRTG	1 cut(s)	2844
Msp20I	TGGCCA	1 cut(s)	2473
MspA1I	CMGCKG	2 cut(s)	2752, 2867
MspCI	CTTAAG	1 cut(s)	1360
MspI	CCGG	4 cut(s)	671, 1848, 2404, 2418
MspR9I	CCNGG	2 cut(s)	672, 2475
MunI	CAATTG	1 cut(s)	600
Mva1269I	GAATGC	3 cut(s)	1270, 1882, 2090
MvaI	CCWGG	1 cut(s)	2475
MvnI	CGCG	1 cut(s)	181
MwoI	GCNNNNNNNGC	9 cut(s)	296, 384, 1287, 1362, 1383, 1486, 2318, 2423, 2752
NaeI	GCCGGC	1 cut(s)	2419
NciI	CCSGG	1 cut(s)	672
NcoI	CCATGG	2 cut(s)	1208, 2625
NgoMIV	GCCGGC	1 cut(s)	2417
NlaIV	GGNNCC	6 cut(s)	59, 174, 547, 906, 1036, 2086
NmuCI	GTSAC	2 cut(s)	2665, 2908
NspI	RCATGY	1 cut(s)	740
PagI	TCATGA	2 cut(s)	1945, 2839
PciI	ACATGT	1 cut(s)	736
PcsI	WCGNNNNNNNCGW	2 cut(s)	221, 251
PctI	GAATGC	3 cut(s)	1270, 1882, 2090
PdiI	GCCGGC	1 cut(s)	2419
PdmI	GAANNNNTTC	1 cut(s)	77
PfeI	GAWTC	8 cut(s)	101, 437, 523, 665, 980, 1248, 1294, 2621
PflFI	GACNNNGTC	2 cut(s)	2671, 2731
PflMI	CCANNNNNTGG	1 cut(s)	1440
PinAI	ACCGGT	1 cut(s)	1847
PkrI	GCNGC	8 cut(s)	120, 274, 1681, 1741, 1819, 2177, 2542, 2754
Ple19I	CGATCG	1 cut(s)	2657
PleI	GAGTC	4 cut(s)	30, 1225, 1442, 1936
PpsI	GAGTC	4 cut(s)	30, 1225, 1442, 1936
Ppu21I	YACGTR	3 cut(s)	325, 337, 2356
PscI	ACATGT	1 cut(s)	736
Psp124BI	GAGCTC	3 cut(s)	782, 2221, 2386
Psp1406I	AACGTT	1 cut(s)	537
Psp6I	CCWGG	1 cut(s)	2473
PspGI	CCWGG	1 cut(s)	2473
PspN4I	GGNNCC	6 cut(s)	59, 174, 547, 906, 1036, 2086
PspPI	GGNCC	2 cut(s)	163, 2611
PsrI	GAACNNNNNNTAC	2 cut(s)	2040, 2072
PstI	CTGCAG	1 cut(s)	2214
PstNI	CAGNNNCTG	1 cut(s)	2573
PsuI	RGATCY	2 cut(s)	1052, 1621
PsyI	GACNNNGTC	2 cut(s)	2671, 2731
PvuI	CGATCG	1 cut(s)	2657
PvuII	CAGCTG	1 cut(s)	2752
RsaI	GTAC	7 cut(s)	59, 86, 335, 872, 1758, 1826, 2058
RsaNI	GTAC	7 cut(s)	58, 85, 334, 871, 1757, 1825, 2057
RseI	CAYNNNNRTG	1 cut(s)	2844
SacI	GAGCTC	3 cut(s)	782, 2221, 2386
SalI	GTCGAC	1 cut(s)	481
SaqAI	TTAA	7 cut(s)	204, 534, 1085, 1361, 2181, 2240, 2493
SatI	GCNGC	8 cut(s)	119, 273, 1680, 1740, 1818, 2176, 2541, 2753
Sau96I	GGNCC	2 cut(s)	163, 2611
ScaI	AGTACT	1 cut(s)	1758
SchI	GAGTC	4 cut(s)	30, 1226, 1442, 1936
ScrFI	CCNGG	2 cut(s)	672, 2475
SduI	GDGCHC	4 cut(s)	586, 782, 2221, 2386
SfaNI	GCATC	7 cut(s)	757, 1247, 1773, 2255, 2404, 2646, 2686
SfcI	CTRYAG	2 cut(s)	2210, 2258
SinI	GGWCC	1 cut(s)	163
SmiMI	CAYNNNNRTG	1 cut(s)	2844
SmlI	CTYRAG	4 cut(s)	614, 1043, 1360, 1809
SmoI	CTYRAG	4 cut(s)	614, 1043, 1360, 1809
SnaBI	TACGTA	1 cut(s)	325
SpeI	ACTAGT	1 cut(s)	260
SsiI	CCGC	4 cut(s)	1817, 2088, 2175, 2867
SspMI	CTAG	9 cut(s)	261, 558, 1158, 1530, 1745, 1760, 1898, 2150, 2756
SstI	GAGCTC	3 cut(s)	782, 2221, 2386
StyD4I	CCNGG	2 cut(s)	670, 2473
StyI	CCWWGG	4 cut(s)	756, 1208, 1434, 2625
TaaI	ACNGT	8 cut(s)	511, 791, 1276, 1795, 2342, 2465, 2575, 2671
TaiI	ACGT	5 cut(s)	327, 339, 540, 2351, 2358
TatI	WGTACW	4 cut(s)	84, 870, 1756, 2056
TauI	GCSGC	2 cut(s)	1820, 2178
TfiI	GAWTC	8 cut(s)	101, 437, 523, 665, 980, 1248, 1294, 2621
Tru1I	TTAA	7 cut(s)	204, 534, 1085, 1361, 2181, 2240, 2493
Tru9I	TTAA	7 cut(s)	204, 534, 1085, 1361, 2181, 2240, 2493
TscAI	CASTG	7 cut(s)	31, 298, 516, 2146, 2299, 2578, 2817
TseFI	GTSAC	2 cut(s)	2665, 2908
TseI	GCWGC	6 cut(s)	118, 272, 1679, 1739, 2540, 2752
Tsp45I	GTSAC	2 cut(s)	2665, 2908
TspGWI	ACGGA	2 cut(s)	356, 2561
TspRI	CASTG	7 cut(s)	31, 298, 516, 2146, 2299, 2578, 2817
Tth111I	GACNNNGTC	2 cut(s)	2671, 2731
Van91I	CCANNNNNTGG	1 cut(s)	1440
Vha464I	CTTAAG	1 cut(s)	1360
VpaK11BI	GGWCC	1 cut(s)	163
XapI	RAATTY	7 cut(s)	226, 517, 553, 1012, 1542, 1635, 2479
XceI	RCATGY	1 cut(s)	740
XcmI	CCANNNNNNNNNTGG	2 cut(s)	298, 1441
XmiI	GTMKAC	3 cut(s)	482, 2125, 2298
XmnI	GAANNNNTTC	1 cut(s)	77
XspI	CTAG	9 cut(s)	261, 558, 1158, 1530, 1745, 1760, 1898, 2150, 2756
ZrmI	AGTACT	1 cut(s)	1758

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.