pycom10g19790

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006392
Physical Location & Seq
Reverse (-)
22853967 .. 22856052
2086 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g19790.1

Sequence Viewer

Length: 1857 bp
ATGGCTTCCTTGAAGGAACTGCTCACCCAAGAACGAGTCGGTCTCGAAAAAAATAAGTACCCGAAAGGCCAGAAACCATTGATGTACGGAGAGAGAGATGAACTTACGGCTCTATTTCTGCCTGTGCACATCTGCCAGGATCGAAAAAGTTACGATTTTTCGCACCACAAGGCTCGGACGCGAAAAGGGTCGTCGAGAAGGGTGAGTACTACTTCGGAGAGGTCGAATTCCAAGTCATTGGTTTCGGAGAGTTCGATGGATGGTGAGCCTGCCGCCATTGATGAAGTTACCACTAGAGCTGTGGTTTCGATACTTAGCGGTTACGTTGGGAGGTACGTGAAGGATGAGGGTTTTCGGGAAACAATCCGCGAAAAGTGCAGGTCTTGCTTGGTGAGAAAGAAAAAGGATCTGGATAATGGGATGTTGGGAAACTTGGAACTGGGTATTGAAAGTGTTGAGAAGTTGGTGGAAGATCAATGGTCGGAAAGTAAGGAAGTTAGGACGAAAAAAGTGAAGAATACGATTCGGGTTTTGAACGTTGTTGGTTCCTCAAAATCTAGCAGTTCTCATCTCTCTGCCTGTGCTCAGCTCTACTTGTCGATTGTTCACAAGATTGAGAAAAATGACCGGCTTTCGGCGAGGCACTTGCTTCAAGTGTTCTGTGATTCGGCGAGTTCGGCACGAACGCATTTTCTTCCCGATCTTTGGGAGCATTTCTTTCTTCCACATCTTTTACATGTGAAGATTTGGTATGCCAGGGAAATGGATGTTCTTTCGAGCTCAGATGACGGTGAGAAGGAGAAGAAAATGAAAGCCGTGGCAAGAGCGTACGATGAACAAATGGATATGGGAACTACAAAGTTTGCTTTGTACTACAAAGAGTGGCTTCAAGTTGGGGCTGAGGCCCCTCCTGTTCCTCCTACTATACCTTTGCCTTCTTCGATACGCAGTTGCAGATCATCGAGAAGGAGATCATCGGATTCTTACACTTCACATTCCTCGCTCAACAAAAATTTATACCACGCTGTGTTTGGCCCCACGCCTGAGCGAACATCTGTGGAGCTCAATCAGCGAAATGGAGTTTTGAACGATGCTTGGGGTCTAGATGAGGTGGAAAAGTTGTGTGTAGATGAAGATAATTACAACCATATCGGTTATGTTCATAGTGGAGAAAGGTCTAGTCGGAGAAGGTCATCAAGCGAAAATCATAGAAATCCAGTAATTGCGCTATGGCCTGAGTCGGAGACACAGAAGACGGGCCATTTTGGATTCTTTAGATGCCAGAATGAGCCAACAGAATGCTTGGTGGGCAAGAATCTCGTAGTCAAGAGCAATTCAATCCGTAACCAAGATAACTCCCATCTTCCACCTCTAAGCAACTTAAGCCGGGCTATTAGCACCATCTACTCCTCCGATAATCTAAGTGATTGCGAAGGTGCAATTCGTGTGATTACCAAGGCTTGGTTGGACTCCCATGGTGATCCTGTCATTGAGGTTGAGCTATCAAATCCACCTGTTATTCAGGGAATGCTGGAGGTGTTGTTTGTTTCTAGTAATGACGAAATTTTGGAATTAGTAATCTTGGTTTTAGCTGAATTTGTAGCGAGAAACGAGATGATTAGACAGATCATATTGAACTCGGATCCACAGCTTGAGTTTCTTATGAGACTTTTAAAAAGTAATGGTCTATTTTTGAAAGCTGCAATTCTGCTTTACTTGTTGAAGCCAAAGGCAAAACAGATGATATCTGTTGAATGGGTGGCACTAGTCCTTCGAGTACTAGAGTTTGGATCGATCAGTTGCAGACACTATTCACAGTTCGTTGCAGTCCTCAAGTGGCGGCGTTGTACCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

619

Amino Acids

70.16

Weight (kDa)

8.72

Isoelectric Point (pI)

50.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 97 - 313 2.3e-90 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_2nd PF23654 458 - 598 1.3e-50 E3 ubiquitin-protein ligase LIN-like, ARM repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 369
AccB7I CCANNNNNTGG 1 cut(s) 1461
AccII CGCG 2 cut(s) 181, 369
AciI CCGC 4 cut(s) 273, 318, 367, 1840
AclI AACGTT 1 cut(s) 537
AclWI GGATC 6 cut(s) 147, 414, 1475, 1637, 1650, 1798
AcsI RAATTY 4 cut(s) 226, 1012, 1563, 1595
AdeI CACNNNGTG 1 cut(s) 1027
AfaI GTAC 8 cut(s) 59, 86, 208, 335, 830, 872, 1779, 1849
AfiI CCNNNNNNNGG 3 cut(s) 634, 705, 1461
AflII CTTAAG 1 cut(s) 1381
AflIII ACRYGT 1 cut(s) 736
AhlI ACTAGT 1 cut(s) 1765
AjnI CCWGG 2 cut(s) 135, 755
AluBI AGCT 8 cut(s) 299, 589, 780, 1063, 1501, 1592, 1651, 1700
AluI AGCT 8 cut(s) 299, 589, 780, 1063, 1501, 1592, 1651, 1700
Alw21I GWGCWC 4 cut(s) 129, 586, 782, 1065
Alw26I GTCTC 3 cut(s) 47, 1238, 1660
Alw44I GTGCAC 1 cut(s) 125
AlwI GGATC 6 cut(s) 147, 414, 1475, 1637, 1650, 1798
AoxI GGCC 5 cut(s) 67, 903, 1033, 1232, 1258
ApaLI GTGCAC 1 cut(s) 125
ApeKI GCWGC 1 cut(s) 1700
ApoI RAATTY 4 cut(s) 226, 1012, 1563, 1595
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
AspLEI GCGC 1 cut(s) 1228
AspS9I GGNCC 3 cut(s) 904, 1034, 1258
AsuC2I CCSGG 1 cut(s) 1388
AsuHPI GGTGA 6 cut(s) 16, 214, 275, 403, 803, 1490
BaeGI GKGCMC 1 cut(s) 129
BamHI GGATCC 1 cut(s) 1642
BanII GRGCYC 2 cut(s) 782, 1065
BarI GAAGNNNNNNTAC 2 cut(s) 190, 222
BbsI GAAGAC 1 cut(s) 1259
Bbv12I GWGCWC 4 cut(s) 129, 586, 782, 1065
BbvCI CCTCAGC 1 cut(s) 900
BbvI GCAGC 1 cut(s) 1687
BccI CCATC 4 cut(s) 250, 254, 1368, 1409
BceAI ACGGC 2 cut(s) 123, 800
BcgI CGANNNNNNTGC 4 cut(s) 628, 662, 1300, 1334
BciT130I CCWGG 2 cut(s) 137, 757
BcnI CCSGG 1 cut(s) 1388
BcoDI GTCTC 3 cut(s) 47, 1238, 1660
BcuI ACTAGT 1 cut(s) 1765
BfaI CTAG 7 cut(s) 294, 558, 1103, 1179, 1551, 1766, 1781
BfrI CTTAAG 1 cut(s) 1381
BfuAI ACCTGC 1 cut(s) 369
BisI GCNGC 3 cut(s) 273, 1701, 1841
BlpI GCTNAGC 1 cut(s) 585
BlsI GCNGC 3 cut(s) 274, 1702, 1842
BmcAI AGTACT 2 cut(s) 208, 1779
Bme1390I CCNGG 3 cut(s) 137, 757, 1388
BmgT120I GGNCC 3 cut(s) 904, 1034, 1258
BmiI GGNNCC 4 cut(s) 547, 906, 1036, 1644
BmrFI CCNGG 3 cut(s) 137, 757, 1388
BmrI ACTGGG 1 cut(s) 449
BmsI GCATC 2 cut(s) 1081, 1268
BmuI ACTGGG 1 cut(s) 449
BpiI GAAGAC 1 cut(s) 1259
BplI GAGNNNNNCTC 2 cut(s) 27, 59
BpmI CTGGAG 1 cut(s) 1553
Bpu10I CCTNAGC 2 cut(s) 900, 1044
Bpu1102I GCTNAGC 1 cut(s) 585
BpuEI CTTGAG 2 cut(s) 1673, 1817
BpuMI CCSGG 1 cut(s) 1388
Bsa29I ATCGAT 1 cut(s) 1793
BsaAI YACGTR 1 cut(s) 337
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 4 cut(s) 756, 816, 1455, 1474
BsaXI ACNNNNNCTCC 2 cut(s) 1526, 1556
Bsc4I CCNNNNNNNGG 3 cut(s) 634, 705, 1461
Bse118I RCCGGY 1 cut(s) 627
Bse1I ACTGG 2 cut(s) 444, 1217
BseBI CCWGG 2 cut(s) 137, 757
BseCI ATCGAT 1 cut(s) 1793
BseDI CCNNGG 4 cut(s) 756, 816, 1455, 1474
BseGI GGATG 4 cut(s) 265, 349, 426, 772
BseLI CCNNNNNNNGG 3 cut(s) 634, 705, 1461
BseMII CTCAG 5 cut(s) 599, 795, 891, 1035, 1227
BseNI ACTGG 2 cut(s) 444, 1217
BseRI GAGGAG 1 cut(s) 1399
BseSI GKGCMC 1 cut(s) 129
BseXI GCAGC 1 cut(s) 1687
BsgI GTGCAG 1 cut(s) 397
Bsh1236I CGCG 2 cut(s) 181, 369
BshFI GGCC 5 cut(s) 69, 905, 1035, 1234, 1260
BshVI ATCGAT 1 cut(s) 1793
BsiHKAI GWGCWC 4 cut(s) 129, 586, 782, 1065
BsiSI CCGG 2 cut(s) 628, 1387
BsiWI CGTACG 1 cut(s) 828
BslI CCNNNNNNNGG 3 cut(s) 634, 705, 1461
BsmAI GTCTC 3 cut(s) 47, 1238, 1660
BsmI GAATGC 2 cut(s) 1304, 1533
BsnI GGCC 5 cut(s) 69, 905, 1035, 1234, 1260
Bso31I GGTCTC 1 cut(s) 47
Bsp1286I GDGCHC 4 cut(s) 129, 586, 782, 1065
Bsp1720I GCTNAGC 1 cut(s) 585
Bsp19I CCATGG 1 cut(s) 1474
BspACI CCGC 4 cut(s) 273, 318, 367, 1840
BspANI GGCC 5 cut(s) 69, 905, 1035, 1234, 1260
BspCNI CTCAG 5 cut(s) 598, 794, 892, 1036, 1228
BspDI ATCGAT 1 cut(s) 1793
BspFNI CGCG 2 cut(s) 181, 369
BspLI GGNNCC 4 cut(s) 547, 906, 1036, 1644
BspMI ACCTGC 1 cut(s) 369
BspPI GGATC 6 cut(s) 147, 414, 1475, 1637, 1650, 1798
BspTI CTTAAG 1 cut(s) 1381
BspTNI GGTCTC 1 cut(s) 47
BsrFI RCCGGY 1 cut(s) 627
BsrI ACTGG 2 cut(s) 444, 1217
BssAI RCCGGY 1 cut(s) 627
BssECI CCNNGG 4 cut(s) 756, 816, 1455, 1474
BssT1I CCWWGG 2 cut(s) 1455, 1474
Bst2UI CCWGG 2 cut(s) 137, 757
Bst4CI ACNGT 2 cut(s) 791, 1818
BstAFI CTTAAG 1 cut(s) 1381
BstAPI GCANNNNNTGC 1 cut(s) 384
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 8 cut(s) 314, 585, 781, 900, 1044, 1236, 1373, 1421
BstDSI CCRYGG 2 cut(s) 816, 1474
BstF5I GGATG 4 cut(s) 265, 349, 426, 772
BstFNI CGCG 2 cut(s) 181, 369
BstHHI GCGC 1 cut(s) 1228
BstMAI GTCTC 3 cut(s) 47, 1238, 1660
BstMWI GCNNNNNNNGC 6 cut(s) 375, 384, 677, 1069, 1308, 1383
BstNI CCWGG 2 cut(s) 137, 757
BstNSI RCATGY 1 cut(s) 740
BstSCI CCNGG 3 cut(s) 135, 755, 1386
BstSLI GKGCMC 1 cut(s) 129
BstUI CGCG 2 cut(s) 181, 369
BstV1I GCAGC 1 cut(s) 1687
BstV2I GAAGAC 1 cut(s) 1259
BstX2I RGATCY 2 cut(s) 406, 1642
BstXI CCANNNNNNTGG 2 cut(s) 238, 763
BstYI RGATCY 2 cut(s) 406, 1642
Bsu15I ATCGAT 1 cut(s) 1793
BsuRI GGCC 5 cut(s) 69, 905, 1035, 1234, 1260
BsuTUI ATCGAT 1 cut(s) 1793
BtgI CCRYGG 2 cut(s) 816, 1474
BtsCI GGATG 4 cut(s) 265, 349, 426, 772
BveI ACCTGC 1 cut(s) 369
Cac8I GCNNGC 1 cut(s) 270
CfoI GCGC 1 cut(s) 1228
Cfr10I RCCGGY 1 cut(s) 627
Cfr13I GGNCC 3 cut(s) 904, 1034, 1258
ClaI ATCGAT 1 cut(s) 1793
CseI GACGC 1 cut(s) 187
Csp6I GTAC 8 cut(s) 58, 85, 207, 334, 829, 871, 1778, 1848
CviAII CATG 2 cut(s) 737, 1475
CviQI GTAC 8 cut(s) 58, 85, 207, 334, 829, 871, 1778, 1848
DdeI CTNAG 8 cut(s) 314, 585, 781, 900, 1044, 1236, 1373, 1421
DraI TTTAAA 1 cut(s) 1674
DraIII CACNNNGTG 1 cut(s) 1027
Ecl136II GAGCTC 2 cut(s) 780, 1063
Eco130I CCWWGG 2 cut(s) 1455, 1474
Eco24I GRGCYC 2 cut(s) 782, 1065
Eco31I GGTCTC 1 cut(s) 47
Eco32I GATATC 1 cut(s) 1746
Eco53kI GAGCTC 2 cut(s) 780, 1063
EcoICRI GAGCTC 2 cut(s) 780, 1063
EcoO109I RGGNCCY 1 cut(s) 904
EcoRI GAATTC 1 cut(s) 226
EcoRII CCWGG 2 cut(s) 135, 755
EcoRV GATATC 1 cut(s) 1746
EcoT14I CCWWGG 2 cut(s) 1455, 1474
EcoT38I GRGCYC 2 cut(s) 782, 1065
ErhI CCWWGG 2 cut(s) 1455, 1474
FaeI CATG 2 cut(s) 740, 1478
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FatI CATG 2 cut(s) 736, 1474
Fnu4HI GCNGC 3 cut(s) 273, 1701, 1841
FokI GGATG 4 cut(s) 272, 356, 433, 779
FriOI GRGCYC 2 cut(s) 782, 1065
Fsp4HI GCNGC 3 cut(s) 273, 1701, 1841
FspBI CTAG 7 cut(s) 294, 558, 1103, 1179, 1551, 1766, 1781
GlaI GCGC 1 cut(s) 1227
GluI GCNGC 3 cut(s) 273, 1701, 1841
GsuI CTGGAG 1 cut(s) 1553
HaeIII GGCC 5 cut(s) 69, 905, 1035, 1234, 1260
HapII CCGG 2 cut(s) 628, 1387
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 1228
Hin1II CATG 2 cut(s) 740, 1478
Hin6I GCGC 1 cut(s) 1226
HinP1I GCGC 1 cut(s) 1226
HinfI GANTC 8 cut(s) 36, 523, 665, 980, 1238, 1269, 1315, 1469
HpaII CCGG 2 cut(s) 628, 1387
HphI GGTGA 6 cut(s) 16, 214, 275, 403, 803, 1490
Hpy166II GTNNAC 2 cut(s) 127, 607
Hpy188III TCNNGA 8 cut(s) 44, 195, 356, 410, 698, 963, 1103, 1327
Hpy8I GTNNAC 2 cut(s) 127, 607
Hpy99I CGWCG 1 cut(s) 196
HpyAV CCTTC 9 cut(s) 7, 192, 334, 790, 945, 960, 1182, 1427, 1781
HpyCH4III ACNGT 2 cut(s) 791, 1818
HpyCH4IV ACGT 3 cut(s) 324, 336, 537
HpyCH4V TGCA 7 cut(s) 127, 378, 954, 1439, 1703, 1803, 1826
HpyF10VI GCNNNNNNNGC 6 cut(s) 375, 384, 677, 1069, 1308, 1383
HpyF3I CTNAG 8 cut(s) 314, 585, 781, 900, 1044, 1236, 1373, 1421
HpySE526I ACGT 3 cut(s) 324, 336, 537
Hsp92II CATG 2 cut(s) 740, 1478
HspAI GCGC 1 cut(s) 1226
LmnI GCTCC 2 cut(s) 709, 1060
Lsp1109I GCAGC 1 cut(s) 1687
LweI GCATC 2 cut(s) 1081, 1268
MaeI CTAG 7 cut(s) 294, 558, 1103, 1179, 1551, 1766, 1781
MaeII ACGT 3 cut(s) 324, 336, 537
MaeIII GTNAC 4 cut(s) 149, 286, 320, 1343
MflI RGATCY 2 cut(s) 406, 1642
MhlI GDGCHC 4 cut(s) 129, 586, 782, 1065
MlyI GAGTC 3 cut(s) 45, 1247, 1463
MmeI TCCRAC 4 cut(s) 462, 1163, 1221, 1446
MseI TTAA 2 cut(s) 1382, 1673
MspCI CTTAAG 1 cut(s) 1381
MspI CCGG 2 cut(s) 628, 1387
MspR9I CCNGG 3 cut(s) 137, 757, 1388
Mva1269I GAATGC 2 cut(s) 1304, 1533
MvaI CCWGG 2 cut(s) 137, 757
MvnI CGCG 2 cut(s) 181, 369
MwoI GCNNNNNNNGC 6 cut(s) 375, 384, 677, 1069, 1308, 1383
NciI CCSGG 1 cut(s) 1388
NcoI CCATGG 1 cut(s) 1474
NlaIII CATG 2 cut(s) 740, 1478
NlaIV GGNNCC 4 cut(s) 547, 906, 1036, 1644
NspI RCATGY 1 cut(s) 740
PciI ACATGT 1 cut(s) 736
PcsI WCGNNNNNNNCGW 2 cut(s) 221, 251
PctI GAATGC 2 cut(s) 1304, 1533
PfeI GAWTC 5 cut(s) 523, 665, 980, 1269, 1315
Pfl23II CGTACG 1 cut(s) 828
PflMI CCANNNNNTGG 1 cut(s) 1461
PkrI GCNGC 3 cut(s) 274, 1702, 1842
PleI GAGTC 3 cut(s) 44, 1246, 1463
PpsI GAGTC 3 cut(s) 44, 1246, 1463
Ppu21I YACGTR 1 cut(s) 337
PscI ACATGT 1 cut(s) 736
Psp124BI GAGCTC 2 cut(s) 782, 1065
Psp1406I AACGTT 1 cut(s) 537
Psp6I CCWGG 2 cut(s) 135, 755
PspGI CCWGG 2 cut(s) 135, 755
PspLI CGTACG 1 cut(s) 828
PspN4I GGNNCC 4 cut(s) 547, 906, 1036, 1644
PspPI GGNCC 3 cut(s) 904, 1034, 1258
PsuI RGATCY 2 cut(s) 406, 1642
RsaI GTAC 8 cut(s) 59, 86, 208, 335, 830, 872, 1779, 1849
RsaNI GTAC 8 cut(s) 58, 85, 207, 334, 829, 871, 1778, 1848
SacI GAGCTC 2 cut(s) 782, 1065
SaqAI TTAA 2 cut(s) 1382, 1673
SatI GCNGC 3 cut(s) 273, 1701, 1841
Sau96I GGNCC 3 cut(s) 904, 1034, 1258
ScaI AGTACT 2 cut(s) 208, 1779
SchI GAGTC 3 cut(s) 45, 1247, 1463
ScrFI CCNGG 3 cut(s) 137, 757, 1388
SduI GDGCHC 4 cut(s) 129, 586, 782, 1065
SfaNI GCATC 2 cut(s) 1081, 1268
SmlI CTYRAG 3 cut(s) 1381, 1652, 1832
SmoI CTYRAG 3 cut(s) 1381, 1652, 1832
SpeI ACTAGT 1 cut(s) 1765
SsiI CCGC 4 cut(s) 273, 318, 367, 1840
SspMI CTAG 7 cut(s) 294, 558, 1103, 1179, 1551, 1766, 1781
SstI GAGCTC 2 cut(s) 782, 1065
StyD4I CCNGG 3 cut(s) 135, 755, 1386
StyI CCWWGG 2 cut(s) 1455, 1474
TaaI ACNGT 2 cut(s) 791, 1818
TaiI ACGT 3 cut(s) 327, 339, 540
TaqII GACCGA 1 cut(s) 29
TatI WGTACW 3 cut(s) 206, 870, 1777
TauI GCSGC 2 cut(s) 275, 1843
TfiI GAWTC 5 cut(s) 523, 665, 980, 1269, 1315
Tru1I TTAA 2 cut(s) 1382, 1673
Tru9I TTAA 2 cut(s) 1382, 1673
TseI GCWGC 1 cut(s) 1700
TspDTI ATGAA 6 cut(s) 114, 297, 824, 849, 1146, 1151
TspGWI ACGGA 2 cut(s) 102, 1331
Van91I CCANNNNNTGG 1 cut(s) 1461
Vha464I CTTAAG 1 cut(s) 1381
VneI GTGCAC 1 cut(s) 125
XapI RAATTY 4 cut(s) 226, 1012, 1563, 1595
XbaI TCTAGA 1 cut(s) 1102
XceI RCATGY 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 3 cut(s) 298, 1028, 1462
XspI CTAG 7 cut(s) 294, 558, 1103, 1179, 1551, 1766, 1781
ZrmI AGTACT 2 cut(s) 208, 1779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.