MD10G1240200.v1.1

Protein prune homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006920
Physical Location & Seq
Forward (+)
33582135 .. 33589014
6880 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1240200.v1.1.491

Sequence Viewer

Length: 1668 bp
ATGAGGTCCAGGAGTTCCAGTCCCGAAGCAGTTGGGCAACTCAATCAGGTGAAGGAGCCATACAACCGTTCCAGAAAGGACCCGTTTTTCGATGGCCTGTTGAGGCTTGATGATCAGAATCCTAGCCCTCCTACTGTTGATTTTCCGGCAATCCCCGGAAGGCGGAACATTTCTCGTACCATGTCGGATATTGGTAACACTCTCACAAGAAAAGGGCAGCTTCCATTCTCTGATGATAGTGTTCTTGAGCCCTTTGAAAGGGCGAAACTTGGTAAAACTGCTTCATTAGCATTCGCTAATGTTCAGCCAGTTTCTAGCTTTGATTTGGGGAAATTAAATGAGCCCGCCATTGAAGCAAATGATCATAAGCATGGTAGAGTAAATGGTCTAGCAAGTGTCACAAGTGGGAAATCATTCAGGGTGAATAGAAGGAAAGAAAACCTGTCCACAGTTTCACTTCCTCAGTCTGCTGCATCGTTTTATAATGGGGACTCGCCGCAATTTGAAATTGTTGCATCGTGTCCAAGCATTTACAAGCTTAATTTGTTTCTTAAAGCCACAAAGGATGAAGTTAAGGCCGGTGTCCCGGGAAAATTCTTACATGTTGTAATGGCTCAAGATGTTGCTGATGTTGGATCTGTTGCTTCAACCGTCAGTTATGCCTTCTACCTGAATGAAACACAAAAATCTGACCAGTTCTGCACAGTGCCTGTCATCAACATGAAGAGGTCGGACATAAATTCTAAAGCTGAACTCAAATGGTTACTTGATTCTTGTCAAATTGATCAGTCATCCTTAATATTCGTGGATGAGATTGATCTGTCATATTATGATCTATTTGGGAGTCTTAAAGTAGTTCTATTAAATGGCCACAAGCTCCCAGCCAAGCAAGAGGCATTGAAAGAGGCTGTAGTGGAAAATTTCAATTGCAAACAGGGAGAATCTGTATATCCCTGGGTTAAGAGTGTTACTGTGGCAGAGGATAGCTCATGCTGTACAGTTGTTGCTGAGAAGTTTTCTCTGATTTCACCTGAGATTTTGGCTGGACAAGGATTCAGTAGACTTTTGTTAGCTGCCATCCTTTTAGACTCCACAAACTTGTGTAGTTCTCATTGTTCAACCAAGGATAAGTACATGTCTGCACTGTTAATCCATGGTGCTGGTCGCTTTGGATGCAATGGCCTTTACCAGTTATTGAGATACAAAATGTACGATGTATCTAATCTCAAGATTAGTGATATATTGCGCAAGGATTTCAAAAAGTGGACAAGAGTAGGTAAACTGGAGGGTCTTGGTGCTAGAACATCACAAATCGGGATGAGTTCCACCGGAATGCCAATTGCACAGCTTCTTGAACATGAGAACACCTCAGTGCGTGAAATTAAAAATTTCCAACAGATGGAGAAACTTCAGCTTCTCGTGGTTGTTTCTGGGTATTATGATTCGAAAAAGAACTTCAAGAGAGAGATCCTAGTGTCAGCAGAGTCAGTGGAGCTCTTGAAGAATCTGCTGTTTTTCTTCAATTCAGATGCTTCCCAACTGCCTCTCAAAGCAATCCATCAGCCAGTTCTCGGAGAGGTGATGCAAGTATTTGAGATTGACAAGGTCACATCAAGAAAGACCATTGAGCGCCTTTTAGAAGAATTTGGTGGGACTTTGAAAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
DHHA2

Protein Analysis

556

Amino Acids

61.58

Weight (kDa)

8.63

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHA2 PF02833 402 - 540 3.6e-10 DHHA2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 483
Acc16I TGCGCA 1 cut(s) 1247
AccB7I CCANNNNNTGG 1 cut(s) 1399
AccI GTMKAC 1 cut(s) 1060
AciI CCGC 3 cut(s) 163, 345, 497
AclWI GGATC 2 cut(s) 643, 1462
AcoI YGGCCR 1 cut(s) 868
AcsI RAATTY 5 cut(s) 593, 739, 919, 1387, 1643
AcuI CTGAAG 1 cut(s) 1394
AfaI GTAC 4 cut(s) 178, 997, 1133, 1211
AfiI CCNNNNNNNGG 4 cut(s) 162, 258, 1399, 1571
AflIII ACRYGT 2 cut(s) 601, 1134
AjnI CCWGG 2 cut(s) 8, 953
AleI CACNNNNGTG 1 cut(s) 1370
Alw21I GWGCWC 1 cut(s) 1497
AlwI GGATC 2 cut(s) 643, 1462
AlwNI CAGNNNCTG 1 cut(s) 710
Ama87I CYCGRG 1 cut(s) 586
AoxI GGCC 4 cut(s) 94, 576, 868, 1180
ApeKI GCWGC 3 cut(s) 217, 470, 1073
ApoI RAATTY 5 cut(s) 593, 739, 919, 1387, 1643
ArsI GACNNNNNNTTYG 2 cut(s) 71, 103
Asp700I GAANNNNTTC 1 cut(s) 413
AspLEI GCGC 2 cut(s) 1248, 1632
AspS9I GGNCC 2 cut(s) 6, 79
AsuC2I CCSGG 3 cut(s) 156, 587, 588
AsuHPI GGTGA 4 cut(s) 61, 433, 1020, 1591
AsuII TTCGAA 1 cut(s) 1445
AvaI CYCGRG 1 cut(s) 586
AvaII GGWCC 2 cut(s) 6, 79
BalI TGGCCA 1 cut(s) 870
BanII GRGCYC 3 cut(s) 252, 345, 1497
BarI GAAGNNNNNNTAC 4 cut(s) 44, 76, 265, 297
BauI CACGAG 1 cut(s) 1418
Bbv12I GWGCWC 1 cut(s) 1497
BbvI GCAGC 3 cut(s) 229, 457, 1060
BccI CCATC 4 cut(s) 86, 1085, 1393, 1566
BciT130I CCWGG 2 cut(s) 10, 955
BclI TGATCA 3 cut(s) 112, 361, 784
BcnI CCSGG 3 cut(s) 156, 587, 588
BfaI CTAG 5 cut(s) 123, 315, 389, 1299, 1472
BfmI CTRYAG 1 cut(s) 909
BfoI RGCGCY 1 cut(s) 1633
BisI GCNGC 4 cut(s) 218, 471, 497, 1074
BlsI GCNGC 4 cut(s) 219, 472, 498, 1075
Bme1390I CCNGG 5 cut(s) 10, 156, 587, 588, 955
Bme18I GGWCC 2 cut(s) 6, 79
BmeT110I CYCGRG 1 cut(s) 586
BmgT120I GGNCC 2 cut(s) 6, 79
BmiI GGNNCC 2 cut(s) 57, 81
BmrFI CCNGG 5 cut(s) 10, 156, 587, 588, 955
BmsI GCATC 5 cut(s) 482, 524, 1163, 1519, 1572
BplI GAGNNNNNCTC 4 cut(s) 971, 1003, 1352, 1384
BpmI CTGGAG 1 cut(s) 1304
Bpu14I TTCGAA 1 cut(s) 1445
BpuEI CTTGAG 3 cut(s) 266, 600, 1211
BpuMI CCSGG 3 cut(s) 156, 587, 588
BsaBI GATNNNNATC 1 cut(s) 117
BsaJI CCNNGG 6 cut(s) 154, 586, 953, 954, 1122, 1153
BsaWI WCCGGW 1 cut(s) 1328
BsaXI ACNNNNNCTCC 3 cut(s) 34, 930, 960
Bsc4I CCNNNNNNNGG 4 cut(s) 162, 258, 1399, 1571
Bse118I RCCGGY 1 cut(s) 578
Bse1I ACTGG 6 cut(s) 18, 308, 694, 1189, 1287, 1565
Bse3DI GCAATG 1 cut(s) 1183
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 2 cut(s) 10, 955
BseDI CCNNGG 6 cut(s) 154, 586, 953, 954, 1122, 1153
BseGI GGATG 6 cut(s) 571, 791, 814, 1077, 1178, 1323
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 4 cut(s) 162, 258, 1399, 1571
BseMI GCAATG 1 cut(s) 1183
BseMII CTCAG 4 cut(s) 476, 999, 1023, 1383
BseNI ACTGG 6 cut(s) 18, 308, 694, 1189, 1287, 1565
BseXI GCAGC 3 cut(s) 229, 457, 1060
BseYI CCCAGC 1 cut(s) 880
BsgI GTGCAG 2 cut(s) 685, 1125
BshFI GGCC 4 cut(s) 96, 578, 870, 1182
BsiHKAI GWGCWC 1 cut(s) 1497
BsiHKCI CYCGRG 1 cut(s) 586
BsiSI CCGG 5 cut(s) 146, 156, 579, 587, 1329
BslFI GGGAC 3 cut(s) 6, 503, 569
BslI CCNNNNNNNGG 4 cut(s) 162, 258, 1399, 1571
BsmFI GGGAC 3 cut(s) 6, 503, 569
BsmI GAATGC 2 cut(s) 290, 1338
BsnI GGCC 4 cut(s) 96, 578, 870, 1182
BsoBI CYCGRG 1 cut(s) 586
Bsp119I TTCGAA 1 cut(s) 1445
Bsp1286I GDGCHC 3 cut(s) 252, 345, 1497
Bsp1407I TGTACA 1 cut(s) 995
Bsp143I GATC 7 cut(s) 112, 361, 635, 784, 817, 832, 1467
Bsp19I CCATGG 1 cut(s) 1153
BspACI CCGC 3 cut(s) 163, 345, 497
BspANI GGCC 4 cut(s) 96, 578, 870, 1182
BspCNI CTCAG 4 cut(s) 475, 1000, 1024, 1382
BspLI GGNNCC 2 cut(s) 57, 81
BspPI GGATC 2 cut(s) 643, 1462
BspT104I TTCGAA 1 cut(s) 1445
BsrDI GCAATG 1 cut(s) 1183
BsrFI RCCGGY 1 cut(s) 578
BsrGI TGTACA 1 cut(s) 995
BsrI ACTGG 6 cut(s) 18, 308, 694, 1189, 1287, 1565
BssAI RCCGGY 1 cut(s) 578
BssECI CCNNGG 6 cut(s) 154, 586, 953, 954, 1122, 1153
BssMI GATC 7 cut(s) 112, 361, 635, 784, 817, 832, 1467
BssSI CACGAG 1 cut(s) 1418
BssT1I CCWWGG 2 cut(s) 1122, 1153
Bst2BI CACGAG 1 cut(s) 1418
Bst2UI CCWGG 2 cut(s) 10, 955
Bst4CI ACNGT 8 cut(s) 68, 136, 451, 652, 706, 973, 1000, 1146
Bst6I CTCTTC 1 cut(s) 719
BstAUI TGTACA 1 cut(s) 995
BstBI TTCGAA 1 cut(s) 1445
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 4 cut(s) 462, 1008, 1032, 1369
BstDSI CCRYGG 1 cut(s) 1153
BstENI CCTNNNNNAGG 1 cut(s) 256
BstF5I GGATG 6 cut(s) 571, 791, 814, 1077, 1178, 1323
BstH2I RGCGCY 1 cut(s) 1633
BstHHI GCGC 2 cut(s) 1248, 1632
BstKTI GATC 7 cut(s) 115, 364, 638, 787, 820, 835, 1470
BstMBI GATC 7 cut(s) 112, 361, 635, 784, 817, 832, 1467
BstMWI GCNNNNNNNGC 3 cut(s) 287, 353, 1173
BstNI CCWGG 2 cut(s) 10, 955
BstNSI RCATGY 2 cut(s) 605, 1138
BstSCI CCNGG 5 cut(s) 8, 154, 585, 586, 953
BstSFI CTRYAG 1 cut(s) 909
BstV1I GCAGC 3 cut(s) 229, 457, 1060
BstX2I RGATCY 2 cut(s) 635, 1467
BstXI CCANNNNNNTGG 1 cut(s) 1160
BstYI RGATCY 2 cut(s) 635, 1467
BsuRI GGCC 4 cut(s) 96, 578, 870, 1182
BtgI CCRYGG 1 cut(s) 1153
BtsCI GGATG 6 cut(s) 571, 791, 814, 1077, 1178, 1323
BtsIMutI CAGTG 4 cut(s) 711, 1142, 1377, 1494
Cac8I GCNNGC 1 cut(s) 345
CaiI CAGNNNCTG 1 cut(s) 710
CfoI GCGC 2 cut(s) 1248, 1632
Cfr10I RCCGGY 1 cut(s) 578
Cfr13I GGNCC 2 cut(s) 6, 79
Cfr9I CCCGGG 1 cut(s) 586
Csp6I GTAC 4 cut(s) 177, 996, 1132, 1210
CviAII CATG 8 cut(s) 181, 371, 602, 721, 990, 1135, 1154, 1358
CviQI GTAC 4 cut(s) 177, 996, 1132, 1210
DdeI CTNAG 4 cut(s) 462, 1008, 1032, 1369
DpnI GATC 7 cut(s) 114, 363, 637, 786, 819, 834, 1469
DpnII GATC 7 cut(s) 112, 361, 635, 784, 817, 832, 1467
EaeI YGGCCR 1 cut(s) 868
Eam1104I CTCTTC 1 cut(s) 719
EarI CTCTTC 1 cut(s) 719
EciI GGCGGA 1 cut(s) 178
Ecl136II GAGCTC 1 cut(s) 1495
Eco130I CCWWGG 2 cut(s) 1122, 1153
Eco24I GRGCYC 3 cut(s) 252, 345, 1497
Eco47I GGWCC 2 cut(s) 6, 79
Eco53kI GAGCTC 1 cut(s) 1495
Eco57I CTGAAG 1 cut(s) 1394
Eco88I CYCGRG 1 cut(s) 586
EcoICRI GAGCTC 1 cut(s) 1495
EcoNI CCTNNNNNAGG 1 cut(s) 256
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 2 cut(s) 8, 953
EcoT14I CCWWGG 2 cut(s) 1122, 1153
EcoT38I GRGCYC 3 cut(s) 252, 345, 1497
ErhI CCWWGG 2 cut(s) 1122, 1153
FaeI CATG 8 cut(s) 184, 374, 605, 724, 993, 1138, 1157, 1361
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 3 cut(s) 6, 503, 569
FatI CATG 8 cut(s) 180, 370, 601, 720, 989, 1134, 1153, 1357
FauI CCCGC 1 cut(s) 352
FbaI TGATCA 3 cut(s) 112, 361, 784
FblI GTMKAC 1 cut(s) 1060
Fnu4HI GCNGC 4 cut(s) 218, 471, 497, 1074
FokI GGATG 6 cut(s) 578, 778, 821, 1064, 1185, 1330
FriOI GRGCYC 3 cut(s) 252, 345, 1497
Fsp4HI GCNGC 4 cut(s) 218, 471, 497, 1074
FspBI CTAG 5 cut(s) 123, 315, 389, 1299, 1472
FspI TGCGCA 1 cut(s) 1247
GlaI GCGC 2 cut(s) 1247, 1631
GluI GCNGC 4 cut(s) 218, 471, 497, 1074
GsaI CCCAGC 1 cut(s) 884
GsuI CTGGAG 1 cut(s) 1304
HaeII RGCGCY 1 cut(s) 1633
HaeIII GGCC 4 cut(s) 96, 578, 870, 1182
HapII CCGG 5 cut(s) 146, 156, 579, 587, 1329
HhaI GCGC 2 cut(s) 1248, 1632
Hin1II CATG 8 cut(s) 184, 374, 605, 724, 993, 1138, 1157, 1361
Hin6I GCGC 2 cut(s) 1246, 1630
HinP1I GCGC 2 cut(s) 1246, 1630
HindIII AAGCTT 1 cut(s) 536
HpaII CCGG 5 cut(s) 146, 156, 579, 587, 1329
HphI GGTGA 4 cut(s) 61, 433, 1020, 1591
Hpy166II GTNNAC 4 cut(s) 447, 1061, 1266, 1280
Hpy188I TCNGA 8 cut(s) 117, 187, 232, 691, 733, 1023, 1528, 1574
Hpy8I GTNNAC 4 cut(s) 447, 1061, 1266, 1280
HpyAV CCTTC 4 cut(s) 46, 153, 423, 673
HpyCH4III ACNGT 8 cut(s) 68, 136, 451, 652, 706, 973, 1000, 1146
HpyCH4V TGCA 8 cut(s) 473, 515, 702, 930, 1142, 1176, 1343, 1585
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 353, 1173
HpyF3I CTNAG 4 cut(s) 462, 1008, 1032, 1369
Hsp92II CATG 8 cut(s) 184, 374, 605, 724, 993, 1138, 1157, 1361
HspAI GCGC 2 cut(s) 1246, 1630
Ksp22I TGATCA 3 cut(s) 112, 361, 784
Kzo9I GATC 7 cut(s) 112, 361, 635, 784, 817, 832, 1467
LmnI GCTCC 3 cut(s) 55, 882, 1492
Lsp1109I GCAGC 3 cut(s) 229, 457, 1060
LweI GCATC 5 cut(s) 482, 524, 1163, 1519, 1572
MaeI CTAG 5 cut(s) 123, 315, 389, 1299, 1472
MaeIII GTNAC 5 cut(s) 194, 397, 762, 967, 1606
MalI GATC 7 cut(s) 114, 363, 637, 786, 819, 834, 1469
MboI GATC 7 cut(s) 112, 361, 635, 784, 817, 832, 1467
MboII GAAGA 4 cut(s) 736, 1510, 1513, 1652
MfeI CAATTG 2 cut(s) 925, 1338
MflI RGATCY 2 cut(s) 635, 1467
MhlI GDGCHC 3 cut(s) 252, 345, 1497
MlsI TGGCCA 1 cut(s) 870
MluNI TGGCCA 1 cut(s) 870
MlyI GAGTC 4 cut(s) 485, 853, 1082, 1493
MmeI TCCRAC 4 cut(s) 165, 613, 711, 1417
Mox20I TGGCCA 1 cut(s) 870
MroXI GAANNNNTTC 1 cut(s) 413
MscI TGGCCA 1 cut(s) 870
MslI CAYNNNNRTG 4 cut(s) 369, 719, 1331, 1370
Msp20I TGGCCA 1 cut(s) 870
MspI CCGG 5 cut(s) 146, 156, 579, 587, 1329
MspR9I CCNGG 5 cut(s) 10, 156, 587, 588, 955
MunI CAATTG 2 cut(s) 925, 1338
Mva1269I GAATGC 2 cut(s) 290, 1338
MvaI CCWGG 2 cut(s) 10, 955
MwoI GCNNNNNNNGC 3 cut(s) 287, 353, 1173
NciI CCSGG 3 cut(s) 156, 587, 588
NcoI CCATGG 1 cut(s) 1153
NdeII GATC 7 cut(s) 112, 361, 635, 784, 817, 832, 1467
NlaIII CATG 8 cut(s) 184, 374, 605, 724, 993, 1138, 1157, 1361
NlaIV GGNNCC 2 cut(s) 57, 81
NmuCI GTSAC 2 cut(s) 397, 1606
NsbI TGCGCA 1 cut(s) 1247
NspI RCATGY 2 cut(s) 605, 1138
NspV TTCGAA 1 cut(s) 1445
OliI CACNNNNGTG 1 cut(s) 1370
PasI CCCWGGG 1 cut(s) 954
PciI ACATGT 2 cut(s) 601, 1134
PctI GAATGC 2 cut(s) 290, 1338
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 6 cut(s) 118, 770, 941, 1053, 1442, 1504
PflFI GACNNNGTC 1 cut(s) 1604
PflMI CCANNNNNTGG 1 cut(s) 1399
PfoI TCCNGGA 1 cut(s) 8
PkrI GCNGC 4 cut(s) 219, 472, 498, 1075
PleI GAGTC 4 cut(s) 485, 852, 1082, 1492
PpsI GAGTC 4 cut(s) 485, 852, 1082, 1492
PpuMI RGGWCCY 1 cut(s) 79
PscI ACATGT 2 cut(s) 601, 1134
PsiI TTATAA 1 cut(s) 483
Psp124BI GAGCTC 1 cut(s) 1497
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 2 cut(s) 8, 953
PspFI CCCAGC 1 cut(s) 880
PspGI CCWGG 2 cut(s) 8, 953
PspN4I GGNNCC 2 cut(s) 57, 81
PspPI GGNCC 2 cut(s) 6, 79
PspPPI RGGWCCY 1 cut(s) 79
PstNI CAGNNNCTG 1 cut(s) 710
PsuI RGATCY 2 cut(s) 635, 1467
PsyI GACNNNGTC 1 cut(s) 1604
RsaI GTAC 4 cut(s) 178, 997, 1133, 1211
RsaNI GTAC 4 cut(s) 177, 996, 1132, 1210
RseI CAYNNNNRTG 4 cut(s) 369, 719, 1331, 1370
SacI GAGCTC 1 cut(s) 1497
SatI GCNGC 4 cut(s) 218, 471, 497, 1074
Sau3AI GATC 7 cut(s) 112, 361, 635, 784, 817, 832, 1467
Sau96I GGNCC 2 cut(s) 6, 79
SchI GAGTC 4 cut(s) 485, 853, 1082, 1493
ScrFI CCNGG 5 cut(s) 10, 156, 587, 588, 955
SduI GDGCHC 3 cut(s) 252, 345, 1497
SfaNI GCATC 5 cut(s) 482, 524, 1163, 1519, 1572
SfcI CTRYAG 1 cut(s) 909
SfuI TTCGAA 1 cut(s) 1445
SinI GGWCC 2 cut(s) 6, 79
SmaI CCCGGG 1 cut(s) 588
SmiMI CAYNNNNRTG 4 cut(s) 369, 719, 1331, 1370
SmlI CTYRAG 3 cut(s) 245, 615, 1226
SmoI CTYRAG 3 cut(s) 245, 615, 1226
SsiI CCGC 3 cut(s) 163, 345, 497
SspI AATATT 1 cut(s) 801
SspMI CTAG 5 cut(s) 123, 315, 389, 1299, 1472
SstI GAGCTC 1 cut(s) 1497
StyD4I CCNGG 5 cut(s) 8, 154, 585, 586, 953
StyI CCWWGG 2 cut(s) 1122, 1153
TaaI ACNGT 8 cut(s) 68, 136, 451, 652, 706, 973, 1000, 1146
TaqI TCGA 2 cut(s) 90, 1445
TatI WGTACW 2 cut(s) 995, 1131
TauI GCSGC 1 cut(s) 499
TfiI GAWTC 6 cut(s) 118, 770, 941, 1053, 1442, 1504
TscAI CASTG 4 cut(s) 711, 1149, 1377, 1494
TseFI GTSAC 2 cut(s) 397, 1606
TseI GCWGC 3 cut(s) 217, 470, 1073
Tsp45I GTSAC 2 cut(s) 397, 1606
TspDTI ATGAA 4 cut(s) 273, 582, 690, 737
TspMI CCCGGG 1 cut(s) 586
TspRI CASTG 4 cut(s) 711, 1149, 1377, 1494
Tth111I GACNNNGTC 1 cut(s) 1604
Van91I CCANNNNNTGG 1 cut(s) 1399
VpaK11BI GGWCC 2 cut(s) 6, 79
XagI CCTNNNNNAGG 1 cut(s) 256
XapI RAATTY 5 cut(s) 593, 739, 919, 1387, 1643
XceI RCATGY 2 cut(s) 605, 1138
XmaI CCCGGG 1 cut(s) 586
XmiI GTMKAC 1 cut(s) 1060
XmnI GAANNNNTTC 1 cut(s) 413
XspI CTAG 5 cut(s) 123, 315, 389, 1299, 1472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.