Rw5G012120

Protein prune homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006920
Physical Location & Seq
Reverse (-)
14192593 .. 14197583
4991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012120.1

Sequence Viewer

Length: 1722 bp
ATGAGGTCTCCAACAAGCGCGAGACCAAGAGCAGTCGCTCAGCCTCAGAAGGAAACTTACAGTCGTGCAAGAAAGAACCCATTTTTTAATGGACTGATCGATGGGCCTCCTCCGGCGTTTGATTTTAATGTGAGTCACAGCAGGAACATTGCTCGGTCTATGTCAGATATTGGTCATAAACAGCCACAAGATACCTTATTAGATGTGTTTGAACGGTTCAGTCCAAATGAAAAACCCTTTGATCGAATTACTCCAAAGGAAAAATCCTCTTCATTCCGTATAAAGAAGAACCCTTTTGAGTTTAGTCCAGAAAAGAACTTTGAGTGGTCAGAGAATGGTAAATCTGAGTCCTTTGAATTCACAAATGTTCAGACTGTGTTTTGCTCTAACTTGGCTACTGAAGAAAATGATCAGCAGCGTGAGAGTGTATCTAGTAATACAAGTCCCACTGTTGAAAAAACATTTCGTGTGAATAGAAGGAAGGAAAACCTGTCTGCAGTTCCACTTCCTCAGTCTGCAGCTTCATTTTACAATGGCAACTCGCCGCAGTTCGAAATTTCTGAGTCATGTCCAAGCATTAAGAGGCTTAATCGGTATCTGAAAGCCACAAAGGATGAAGTTAATGCTGGTGTTCCGGGAAGATTTTTACATGCTGTAATAGCTCAAGATGTTCCTGATGTCGGATCCCTTGCCTCAACCATCATGTATGCTTTCTACGTGAATGAAACACATAAAAGTGACCATTTATGCACGGTGCCTGTGATCAACATGAAGAGGTCAGACATAAGTTCTCAAAGCGAACTCAAATGGTTGCTTGCTTCTTGTCAAATTGATCACATGTCCTTAATTTTTGTAGATGAGATCGATCTGTCATATTATGATCTATTTGGGAGTCTTAAACTAGTTCTACTAAATGGCTACAAGCTCCCAACCAAGCAAGAGGCATTGAAAGATGCAGTAGTTGAAATTTTCAATTGCGGAAAGGGTGACTCTGTATACCCCTGGGTTGACACTGTTACTGTAGGAGAGGACAGCTCATGCTGTACTCCTATCGCTGAGAAATTTGCGCTGCGCTCACCTGAGATTTTGGCTGGACATGGATTCAGTAGATTTTTGTTAGCTGCCATCCTCTTAGATTCTGCAAACTTGAGCAGTTCTAATTGTTCCATCAAAGATAAATACATGGCTGCAATGCTAATCCATGGTGCTGGTCGCTTTGGATGCGATGGTCTTTACCAGTTATTGAGATACAAGATGTATGATGTAACTGGTCTCAAAGTAGCTGATATATTGCGCAAGGATTTCAAAAAGTGGACACGAGTAGGTAATCAGGACAATACCGGTTCTAGATTGACAGTATCAGATGTTGGGATGAGCTCTATTGGGACGTCAATTGCAGAACTTCTTGATCATGAGGAATCCTCAGTCCATGAAATCAAATATTTTCAACAGGTGGAGAAACTTCGTCTTCTCATGATTGTTTCTGGGTATTATGATTTGAAAAAGAACTTCAAGAGAGAGATCCTAGTGTCTGCTCAATCAGTGGAGCTCATGAGGAATCTGCTGTCTTTCTTCAATTCAAGTGCTTCCCAACTACCTCTGAAAGCTATCCCCAAGCCAGGTCTCGGAGAGCAGATGCAAGCATTTGAGATTGACAAGATCATATCAAGAAAGACCATCGAGCGGCTCTTGGAAGAATTTGGTGGGAATTCAAAAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
DHHA2

Protein Analysis

573

Amino Acids

64.34

Weight (kDa)

7.94

Isoelectric Point (pI)

53.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHA2 PF02833 419 - 558 7.5e-10 DHHA2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1391
Acc16I TGCGCA 1 cut(s) 1295
AccB1I GGYRCC 1 cut(s) 754
AccBSI CCGCTC 1 cut(s) 1684
AccI GTMKAC 1 cut(s) 996
AccII CGCG 1 cut(s) 20
AciI CCGC 3 cut(s) 545, 978, 1684
AclWI GGATC 3 cut(s) 678, 691, 1516
AcsI RAATTY 6 cut(s) 356, 555, 966, 1061, 1697, 1708
AcuI CTGAAG 1 cut(s) 420
AcyI GRCGYC 1 cut(s) 1388
AfaI GTAC 1 cut(s) 1045
AfiI CCNNNNNNNGG 4 cut(s) 680, 1619, 1625, 1683
AflIII ACRYGT 1 cut(s) 837
AgeI ACCGGT 1 cut(s) 1340
AhlI ACTAGT 1 cut(s) 901
AjnI CCWGG 2 cut(s) 1001, 1618
AluBI AGCT 9 cut(s) 521, 662, 925, 1035, 1121, 1283, 1377, 1549, 1607
AluI AGCT 9 cut(s) 521, 662, 925, 1035, 1121, 1283, 1377, 1549, 1607
Alw21I GWGCWC 2 cut(s) 1379, 1551
Alw26I GTCTC 4 cut(s) 12, 16, 1277, 1628
AlwI GGATC 3 cut(s) 678, 691, 1516
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 5 cut(s) 415, 518, 1069, 1121, 1187
ApoI RAATTY 6 cut(s) 356, 555, 966, 1061, 1697, 1708
AsiGI ACCGGT 1 cut(s) 1340
AspLEI GCGC 4 cut(s) 20, 1069, 1074, 1296
AspS9I GGNCC 1 cut(s) 104
AsuC2I CCSGG 1 cut(s) 636
AsuHPI GGTGA 2 cut(s) 998, 1068
AsuII TTCGAA 1 cut(s) 552
BamHI GGATCC 1 cut(s) 683
BanI GGYRCC 1 cut(s) 754
BanII GRGCYC 2 cut(s) 1379, 1551
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
BauI CACGAG 1 cut(s) 1317
BbsI GAAGAC 1 cut(s) 1460
Bbv12I GWGCWC 2 cut(s) 1379, 1551
BbvI GCAGC 5 cut(s) 427, 530, 1056, 1108, 1174
BccI CCATC 6 cut(s) 95, 707, 1133, 1175, 1220, 1685
BciT130I CCWGG 2 cut(s) 1003, 1620
BclI TGATCA 4 cut(s) 409, 762, 832, 1408
BcnI CCSGG 1 cut(s) 636
BcoDI GTCTC 4 cut(s) 12, 16, 1277, 1628
BcuI ACTAGT 1 cut(s) 901
BfaI CTAG 4 cut(s) 432, 902, 1347, 1526
BfmI CTRYAG 3 cut(s) 495, 516, 1020
BisI GCNGC 7 cut(s) 416, 519, 545, 1070, 1122, 1188, 1685
BlpI GCTNAGC 1 cut(s) 39
BlsI GCNGC 7 cut(s) 417, 520, 546, 1071, 1123, 1189, 1686
Bme1390I CCNGG 3 cut(s) 636, 1003, 1620
BmgT120I GGNCC 1 cut(s) 104
BmiI GGNNCC 2 cut(s) 685, 756
BmrFI CCNGG 3 cut(s) 636, 1003, 1620
BmsI GCATC 3 cut(s) 943, 1211, 1626
BpiI GAAGAC 1 cut(s) 1460
BplI GAGNNNNNCTC 4 cut(s) 1019, 1051, 1406, 1438
Bpu1102I GCTNAGC 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 552
BpuEI CTTGAG 2 cut(s) 648, 1168
BpuMI CCSGG 1 cut(s) 636
Bsa29I ATCGAT 2 cut(s) 99, 864
BsaAI YACGTR 1 cut(s) 718
BsaHI GRCGYC 1 cut(s) 1388
BsaI GGTCTC 4 cut(s) 12, 16, 1277, 1628
BsaJI CCNNGG 3 cut(s) 1001, 1002, 1201
BsaWI WCCGGW 1 cut(s) 1340
Bsc4I CCNNNNNNNGG 4 cut(s) 680, 1619, 1625, 1683
Bse118I RCCGGY 1 cut(s) 1340
Bse1I ACTGG 2 cut(s) 1237, 1273
Bse3DI GCAATG 2 cut(s) 147, 1197
BseBI CCWGG 2 cut(s) 1003, 1620
BseCI ATCGAT 2 cut(s) 99, 864
BseDI CCNNGG 3 cut(s) 1001, 1002, 1201
BseGI GGATG 4 cut(s) 619, 1125, 1226, 1377
BseLI CCNNNNNNNGG 4 cut(s) 680, 1619, 1625, 1683
BseMI GCAATG 2 cut(s) 147, 1197
BseMII CTCAG 8 cut(s) 53, 59, 336, 524, 552, 1047, 1071, 1437
BseNI ACTGG 2 cut(s) 1237, 1273
BseRI GAGGAG 1 cut(s) 99
BseXI GCAGC 5 cut(s) 427, 530, 1056, 1108, 1174
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 1 cut(s) 106
BshNI GGYRCC 1 cut(s) 754
BshTI ACCGGT 1 cut(s) 1340
BshVI ATCGAT 2 cut(s) 99, 864
BsiHKAI GWGCWC 2 cut(s) 1379, 1551
BsiSI CCGG 3 cut(s) 113, 635, 1341
BslFI GGGAC 2 cut(s) 429, 1399
BslI CCNNNNNNNGG 4 cut(s) 680, 1619, 1625, 1683
BsmAI GTCTC 4 cut(s) 12, 16, 1277, 1628
BsmFI GGGAC 2 cut(s) 429, 1399
BsnI GGCC 1 cut(s) 106
Bso31I GGTCTC 4 cut(s) 12, 16, 1277, 1628
Bsp119I TTCGAA 1 cut(s) 552
Bsp1286I GDGCHC 2 cut(s) 1379, 1551
Bsp1720I GCTNAGC 1 cut(s) 39
Bsp19I CCATGG 1 cut(s) 1201
BspACI CCGC 3 cut(s) 545, 978, 1684
BspANI GGCC 1 cut(s) 106
BspCNI CTCAG 8 cut(s) 52, 58, 337, 523, 553, 1048, 1072, 1436
BspDI ATCGAT 2 cut(s) 99, 864
BspFNI CGCG 1 cut(s) 20
BspHI TCATGA 3 cut(s) 1411, 1473, 1551
BspLI GGNNCC 2 cut(s) 685, 756
BspMAI CTGCAG 2 cut(s) 499, 520
BspPI GGATC 3 cut(s) 678, 691, 1516
BspT104I TTCGAA 1 cut(s) 552
BspT107I GGYRCC 1 cut(s) 754
BspTNI GGTCTC 4 cut(s) 12, 16, 1277, 1628
BsrBI CCGCTC 1 cut(s) 1684
BsrDI GCAATG 2 cut(s) 147, 1197
BsrFI RCCGGY 1 cut(s) 1340
BsrI ACTGG 2 cut(s) 1237, 1273
BssAI RCCGGY 1 cut(s) 1340
BssECI CCNNGG 3 cut(s) 1001, 1002, 1201
BssNAI GTATAC 1 cut(s) 997
BssNI GRCGYC 1 cut(s) 1388
BssSI CACGAG 1 cut(s) 1317
BssT1I CCWWGG 1 cut(s) 1201
Bst1107I GTATAC 1 cut(s) 997
Bst2BI CACGAG 1 cut(s) 1317
Bst2UI CCWGG 2 cut(s) 1003, 1620
Bst4CI ACNGT 8 cut(s) 62, 216, 376, 451, 754, 1015, 1021, 1357
Bst6I CTCTTC 2 cut(s) 274, 767
BstACI GRCGYC 1 cut(s) 1388
BstBAI YACGTR 1 cut(s) 718
BstBI TTCGAA 1 cut(s) 552
BstC8I GCNNGC 2 cut(s) 816, 1641
BstDEI CTNAG 9 cut(s) 39, 45, 345, 510, 561, 1056, 1080, 1132, 1423
BstDSI CCRYGG 1 cut(s) 1201
BstF5I GGATG 4 cut(s) 619, 1125, 1226, 1377
BstFNI CGCG 1 cut(s) 20
BstHHI GCGC 4 cut(s) 20, 1069, 1074, 1296
BstMAI GTCTC 4 cut(s) 12, 16, 1277, 1628
BstMWI GCNNNNNNNGC 2 cut(s) 659, 1221
BstNI CCWGG 2 cut(s) 1003, 1620
BstNSI RCATGY 2 cut(s) 653, 841
BstSCI CCNGG 3 cut(s) 634, 1001, 1618
BstSFI CTRYAG 3 cut(s) 495, 516, 1020
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 5 cut(s) 427, 530, 1056, 1108, 1174
BstV2I GAAGAC 1 cut(s) 1460
BstX2I RGATCY 2 cut(s) 683, 1521
BstXI CCANNNNNNTGG 1 cut(s) 1208
BstYI RGATCY 2 cut(s) 683, 1521
BstZ17I GTATAC 1 cut(s) 997
Bsu15I ATCGAT 2 cut(s) 99, 864
BsuRI GGCC 1 cut(s) 106
BsuTUI ATCGAT 2 cut(s) 99, 864
BtgI CCRYGG 1 cut(s) 1201
BtgZI GCGATG 1 cut(s) 1239
BtsCI GGATG 4 cut(s) 619, 1125, 1226, 1377
BtsIMutI CAGTG 3 cut(s) 447, 1011, 1548
Cac8I GCNNGC 2 cut(s) 816, 1641
CciI TCATGA 3 cut(s) 1411, 1473, 1551
CfoI GCGC 4 cut(s) 20, 1069, 1074, 1296
Cfr10I RCCGGY 1 cut(s) 1340
Cfr13I GGNCC 1 cut(s) 104
ClaI ATCGAT 2 cut(s) 99, 864
Csp6I GTAC 1 cut(s) 1044
CspAI ACCGGT 1 cut(s) 1340
CviQI GTAC 1 cut(s) 1044
DdeI CTNAG 9 cut(s) 39, 45, 345, 510, 561, 1056, 1080, 1132, 1423
Eam1104I CTCTTC 2 cut(s) 274, 767
EarI CTCTTC 2 cut(s) 274, 767
Ecl136II GAGCTC 2 cut(s) 1377, 1549
Eco130I CCWWGG 1 cut(s) 1201
Eco24I GRGCYC 2 cut(s) 1379, 1551
Eco31I GGTCTC 4 cut(s) 12, 16, 1277, 1628
Eco53kI GAGCTC 2 cut(s) 1377, 1549
Eco57I CTGAAG 1 cut(s) 420
EcoICRI GAGCTC 2 cut(s) 1377, 1549
EcoRI GAATTC 2 cut(s) 356, 1708
EcoRII CCWGG 2 cut(s) 1001, 1618
EcoT14I CCWWGG 1 cut(s) 1201
EcoT38I GRGCYC 2 cut(s) 1379, 1551
ErhI CCWWGG 1 cut(s) 1201
FaqI GGGAC 2 cut(s) 429, 1399
FbaI TGATCA 4 cut(s) 409, 762, 832, 1408
FblI GTMKAC 1 cut(s) 996
Fnu4HI GCNGC 7 cut(s) 416, 519, 545, 1070, 1122, 1188, 1685
FokI GGATG 4 cut(s) 626, 1112, 1233, 1384
FriOI GRGCYC 2 cut(s) 1379, 1551
Fsp4HI GCNGC 7 cut(s) 416, 519, 545, 1070, 1122, 1188, 1685
FspBI CTAG 4 cut(s) 432, 902, 1347, 1526
FspI TGCGCA 1 cut(s) 1295
GlaI GCGC 4 cut(s) 19, 1068, 1073, 1295
GluI GCNGC 7 cut(s) 416, 519, 545, 1070, 1122, 1188, 1685
HaeIII GGCC 1 cut(s) 106
HapII CCGG 3 cut(s) 113, 635, 1341
HhaI GCGC 4 cut(s) 20, 1069, 1074, 1296
Hin1I GRCGYC 1 cut(s) 1388
Hin6I GCGC 4 cut(s) 18, 1067, 1072, 1294
HinP1I GCGC 4 cut(s) 18, 1067, 1072, 1294
HincII GTYRAC 1 cut(s) 1009
HindII GTYRAC 1 cut(s) 1009
HinfI GANTC 9 cut(s) 133, 347, 563, 892, 989, 1101, 1136, 1418, 1558
HpaII CCGG 3 cut(s) 113, 635, 1341
HphI GGTGA 2 cut(s) 998, 1068
Hpy166II GTNNAC 3 cut(s) 997, 1009, 1314
Hpy8I GTNNAC 3 cut(s) 997, 1009, 1314
HpyAV CCTTC 3 cut(s) 43, 471, 475
HpyCH4III ACNGT 8 cut(s) 62, 216, 376, 451, 754, 1015, 1021, 1357
HpyCH4IV ACGT 2 cut(s) 717, 1388
HpyCH4V TGCA 9 cut(s) 68, 497, 518, 750, 956, 1142, 1190, 1397, 1639
HpyF10VI GCNNNNNNNGC 2 cut(s) 659, 1221
HpyF3I CTNAG 9 cut(s) 39, 45, 345, 510, 561, 1056, 1080, 1132, 1423
HpySE526I ACGT 2 cut(s) 717, 1388
Hsp92I GRCGYC 1 cut(s) 1388
HspAI GCGC 4 cut(s) 18, 1067, 1072, 1294
Ksp22I TGATCA 4 cut(s) 409, 762, 832, 1408
LmnI GCTCC 2 cut(s) 930, 1546
Lsp1109I GCAGC 5 cut(s) 427, 530, 1056, 1108, 1174
LweI GCATC 3 cut(s) 943, 1211, 1626
MaeI CTAG 4 cut(s) 432, 902, 1347, 1526
MaeII ACGT 2 cut(s) 717, 1388
MaeIII GTNAC 5 cut(s) 134, 737, 986, 1015, 1264
MbiI CCGCTC 1 cut(s) 1684
MboII GAAGA 8 cut(s) 261, 298, 413, 651, 784, 1460, 1564, 1706
MfeI CAATTG 2 cut(s) 973, 1392
MflI RGATCY 2 cut(s) 683, 1521
MhlI GDGCHC 2 cut(s) 1379, 1551
MlyI GAGTC 5 cut(s) 142, 356, 572, 901, 983
MmeI TCCRAC 2 cut(s) 35, 661
MseI TTAA 7 cut(s) 87, 126, 579, 588, 621, 845, 897
MslI CAYNNNNRTG 1 cut(s) 735
MspI CCGG 3 cut(s) 113, 635, 1341
MspR9I CCNGG 3 cut(s) 636, 1003, 1620
MteI GCGCNGCGC 1 cut(s) 1070
MunI CAATTG 2 cut(s) 973, 1392
MvaI CCWGG 2 cut(s) 1003, 1620
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 659, 1221
NciI CCSGG 1 cut(s) 636
NcoI CCATGG 1 cut(s) 1201
NlaIV GGNNCC 2 cut(s) 685, 756
NmuCI GTSAC 3 cut(s) 134, 737, 986
NsbI TGCGCA 1 cut(s) 1295
NspI RCATGY 2 cut(s) 653, 841
NspV TTCGAA 1 cut(s) 552
PagI TCATGA 3 cut(s) 1411, 1473, 1551
PasI CCCWGGG 1 cut(s) 1002
PciI ACATGT 1 cut(s) 837
PfeI GAWTC 4 cut(s) 1101, 1136, 1418, 1558
PfoI TCCNGGA 1 cut(s) 634
PinAI ACCGGT 1 cut(s) 1340
PkrI GCNGC 7 cut(s) 417, 520, 546, 1071, 1123, 1189, 1686
PleI GAGTC 5 cut(s) 141, 355, 571, 900, 983
PpsI GAGTC 5 cut(s) 141, 355, 571, 900, 983
Ppu21I YACGTR 1 cut(s) 718
PscI ACATGT 1 cut(s) 837
Psp124BI GAGCTC 2 cut(s) 1379, 1551
Psp6I CCWGG 2 cut(s) 1001, 1618
PspGI CCWGG 2 cut(s) 1001, 1618
PspN4I GGNNCC 2 cut(s) 685, 756
PspPI GGNCC 1 cut(s) 104
PstI CTGCAG 2 cut(s) 499, 520
PsuI RGATCY 2 cut(s) 683, 1521
RsaI GTAC 1 cut(s) 1045
RsaNI GTAC 1 cut(s) 1044
RseI CAYNNNNRTG 1 cut(s) 735
SacI GAGCTC 2 cut(s) 1379, 1551
SaqAI TTAA 7 cut(s) 87, 126, 579, 588, 621, 845, 897
SatI GCNGC 7 cut(s) 416, 519, 545, 1070, 1122, 1188, 1685
Sau96I GGNCC 1 cut(s) 104
SchI GAGTC 5 cut(s) 142, 356, 572, 901, 983
ScrFI CCNGG 3 cut(s) 636, 1003, 1620
SduI GDGCHC 2 cut(s) 1379, 1551
SfaNI GCATC 3 cut(s) 943, 1211, 1626
SfcI CTRYAG 3 cut(s) 495, 516, 1020
SfuI TTCGAA 1 cut(s) 552
SmiMI CAYNNNNRTG 1 cut(s) 735
SmlI CTYRAG 2 cut(s) 663, 1147
SmoI CTYRAG 2 cut(s) 663, 1147
SpeI ACTAGT 1 cut(s) 901
SsiI CCGC 3 cut(s) 545, 978, 1684
SspI AATATT 1 cut(s) 1442
SspMI CTAG 4 cut(s) 432, 902, 1347, 1526
SstI GAGCTC 2 cut(s) 1379, 1551
StyD4I CCNGG 3 cut(s) 634, 1001, 1618
StyI CCWWGG 1 cut(s) 1201
TaaI ACNGT 8 cut(s) 62, 216, 376, 451, 754, 1015, 1021, 1357
TaiI ACGT 2 cut(s) 720, 1391
TaqI TCGA 5 cut(s) 99, 244, 552, 864, 1680
TaqII GACCGA 1 cut(s) 144
TatI WGTACW 1 cut(s) 1043
TauI GCSGC 2 cut(s) 547, 1687
TfiI GAWTC 4 cut(s) 1101, 1136, 1418, 1558
Tru1I TTAA 7 cut(s) 87, 126, 579, 588, 621, 845, 897
Tru9I TTAA 7 cut(s) 87, 126, 579, 588, 621, 845, 897
TscAI CASTG 3 cut(s) 454, 1018, 1548
TseFI GTSAC 3 cut(s) 134, 737, 986
TseI GCWGC 5 cut(s) 415, 518, 1069, 1121, 1187
Tsp45I GTSAC 3 cut(s) 134, 737, 986
TspDTI ATGAA 7 cut(s) 243, 261, 513, 630, 738, 785, 1446
TspGWI ACGGA 1 cut(s) 266
TspRI CASTG 3 cut(s) 454, 1018, 1548
XapI RAATTY 6 cut(s) 356, 555, 966, 1061, 1697, 1708
XbaI TCTAGA 1 cut(s) 1346
XceI RCATGY 2 cut(s) 653, 841
XmiI GTMKAC 1 cut(s) 996
XspI CTAG 4 cut(s) 432, 902, 1347, 1526
ZraI GACGTC 1 cut(s) 1389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.