Rroxscaffold_1G00059110

Protein prune homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006920
Physical Location & Seq
Forward (+)
81109098 .. 81114434
5337 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059110.1

Sequence Viewer

Length: 1794 bp
ATGCTCACAGTGGCCTTTTGTGCTCTTGTTTTACTCATGGACTCAGCCGCCTTCCAATGCCTTCAAAGCTTGCTCCTTTGCATTAGTCTTGGGCTCTCTTCTCTTCATTTCCAGCATCATGAGGAAACTTACAGTCGTGCAAGAAAGAACCCATTTTTTAATGGGCTGATCGATGGGCCTCCTCCGGCGTTTGATTTTAATGTGAGTCCCAGCAGGAAGATTGCTCGGTCTGTGTCAGATATTGGTCATAAACAGCCACAAGATACCTTATTAGATATGTTTGAACGGTTCAGTCCAAATGAAAAACCCTTTGATCGAATTACTCCAAAGGAAAAATCCTCTTCATTCAGTATAAAGAAGAATCCTTTTGAGTTTAGTCCAGAAAAGAACTTTGAGTGGTCGGAGAATGATAACGCTGAGTCCTTTGAATTCACAAATGTTCAGACTGTGTTTTGCTCTAACTTGGCTACTGAAGAAAATGATCAGCAGCGTGAGAGTGTATCTAGTAATACAAGTCCCACTGTTGAAAAAACGTTTCATGTGAATAGAAGGAAGGAAAACCTGTCTGCAGTTCCACTTCCTCAGTCTGCAGCTTCATTTTACAATGGCAACTCGCCGCAGTTCGAAATTTCTGAGTCATGTCCAAGCATTAAGAGGCTTAATTGGTATCTGAAAGCCACAAAGGATGAAGTTAATGCTGGTGTTCCGGGAAGATTCTTACGTGCTGTAATAGCTCAAGATGTTCCTGATGTCGGATCCATTGCCTCAACCATCATGTATGCTTTCTACCTGAATGAAACACATAAAAGTGACCATTTATGCACGGTGCCTGTGATCAACATGAAGAGGTCAGACATAAGTTCTCAAAGCGAACTCAAATGGTTGCTTGCTTCTTGTCAAATTGATCACATGTCCTTAATTTTTGTAGATGAGATCGATCTGTCGTATTATGATCTATTTGGGAGTCTTAAACTAGTTCTACTAAATGGCTACAAGCTCCCGACCAAGCAAGAGGCATTGAAAGATGCAGTAGTTGAAATTTTCAATTGCGGAAAGGGTGACTCTGTATACCCCTGGGTTGACACTGTTACTGTAGGAGAGGACAGCTCATGCTGTACTCCTATCGCTGAGAAATTTGCGCTGCTTTCACCTGAGATTTTGGCTGGACATGGATTCAGTAGATTTTTGTTAGCTGCCATCCTCTTAGATTCTGCAAACTTGAGCAGTTCTAATTGTTCCATCAAAGATAAATACATGGCTGCAATGCTAATCCATGGTGCTGGTCGCTTTGGATGCAATGGTCTTTACCAGTTATTGAGATACAAGATGTATGATGTAACTGGTCTCAAAGTAGCTGATATATTGCGCAAGGATTTCAAAAAGTGGACACGAGTAGGTAATCAGGAGAATACTGGTTCTAGATTGACAGTATCACATGTTGGGATGAGTTCTATTGGGATGTCAATTGCAGAACTTCTTGATCATGAGGATTCTTCAGTCCATGAAATCAAATATTTTCAACAGGTGGAGAAACTTCGTCTTCTCATGATTGTTTCTGGGTATTATGATTTGAAAAAGAACTTCAAGAGAGAGATCCTAGTGTCTGCTCAATCAGTGGAGCTCATGAGGAATCTGCTGTCTTTCTTCAATTCAAGTGCTTCCCAACTACCTCTGAAAGCTATCCCCAAGCCAGGTCTCGGAGAGCAGATGCAAGCATTTGAGATTGACAAGATCATATCAAGAAAGACCATCGAGCGACAATTGGAAGAATTTGGTGGGAATTCAAAAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
DHHA2

Protein Analysis

597

Amino Acids

66.97

Weight (kDa)

6.16

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHA2 PF02833 443 - 581 5.2e-10 DHHA2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1367
AccB1I GGYRCC 1 cut(s) 826
AccI GTMKAC 1 cut(s) 1068
AciI CCGC 3 cut(s) 48, 617, 1050
AclI AACGTT 1 cut(s) 533
AclWI GGATC 3 cut(s) 750, 763, 1588
AcsI RAATTY 6 cut(s) 428, 627, 1038, 1133, 1769, 1780
AcuI CTGAAG 2 cut(s) 492, 1479
AfaI GTAC 1 cut(s) 1117
AfiI CCNNNNNNNGG 3 cut(s) 752, 1691, 1697
AflIII ACRYGT 2 cut(s) 909, 1435
AhlI ACTAGT 1 cut(s) 973
AjnI CCWGG 2 cut(s) 1073, 1690
AluBI AGCT 9 cut(s) 69, 593, 734, 997, 1107, 1193, 1355, 1621, 1679
AluI AGCT 9 cut(s) 69, 593, 734, 997, 1107, 1193, 1355, 1621, 1679
Alw21I GWGCWC 2 cut(s) 25, 1623
Alw26I GTCTC 2 cut(s) 1349, 1700
AlwI GGATC 3 cut(s) 750, 763, 1588
AoxI GGCC 2 cut(s) 12, 176
ApeKI GCWGC 5 cut(s) 487, 590, 1141, 1193, 1259
ApoI RAATTY 6 cut(s) 428, 627, 1038, 1133, 1769, 1780
AspLEI GCGC 2 cut(s) 1141, 1368
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 1 cut(s) 708
AsuHPI GGTGA 2 cut(s) 1070, 1140
AsuII TTCGAA 1 cut(s) 624
BamHI GGATCC 1 cut(s) 755
BanI GGYRCC 1 cut(s) 826
BanII GRGCYC 2 cut(s) 96, 1623
BauI CACGAG 1 cut(s) 1389
BbsI GAAGAC 1 cut(s) 1532
Bbv12I GWGCWC 2 cut(s) 25, 1623
BbvI GCAGC 5 cut(s) 499, 602, 1128, 1180, 1246
BccI CCATC 5 cut(s) 167, 779, 1205, 1247, 1757
BciT130I CCWGG 2 cut(s) 1075, 1692
BclI TGATCA 4 cut(s) 481, 834, 904, 1480
BcnI CCSGG 1 cut(s) 708
BcoDI GTCTC 2 cut(s) 1349, 1700
BcuI ACTAGT 1 cut(s) 973
BfaI CTAG 4 cut(s) 504, 974, 1419, 1598
BfmI CTRYAG 3 cut(s) 567, 588, 1092
BisI GCNGC 7 cut(s) 48, 488, 591, 617, 1142, 1194, 1260
BlsI GCNGC 7 cut(s) 49, 489, 592, 618, 1143, 1195, 1261
Bme1390I CCNGG 3 cut(s) 708, 1075, 1692
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 2 cut(s) 757, 828
BmrFI CCNGG 3 cut(s) 708, 1075, 1692
BmsI GCATC 4 cut(s) 124, 1015, 1283, 1698
BpiI GAAGAC 1 cut(s) 1532
BplI GAGNNNNNCTC 2 cut(s) 1091, 1123
Bpu14I TTCGAA 1 cut(s) 624
BpuEI CTTGAG 2 cut(s) 720, 1240
BpuMI CCSGG 1 cut(s) 708
Bsa29I ATCGAT 2 cut(s) 171, 936
BsaAI YACGTR 1 cut(s) 722
BsaI GGTCTC 2 cut(s) 1349, 1700
BsaJI CCNNGG 3 cut(s) 1073, 1074, 1273
Bsc4I CCNNNNNNNGG 3 cut(s) 752, 1691, 1697
Bse1I ACTGG 3 cut(s) 1309, 1345, 1417
Bse3DI GCAATG 3 cut(s) 759, 1269, 1303
BseBI CCWGG 2 cut(s) 1075, 1692
BseCI ATCGAT 2 cut(s) 171, 936
BseDI CCNNGG 3 cut(s) 1073, 1074, 1273
BseGI GGATG 5 cut(s) 691, 1197, 1298, 1449, 1464
BseLI CCNNNNNNNGG 3 cut(s) 752, 1691, 1697
BseMI GCAATG 3 cut(s) 759, 1269, 1303
BseMII CTCAG 6 cut(s) 57, 408, 596, 624, 1119, 1143
BseNI ACTGG 3 cut(s) 1309, 1345, 1417
BseRI GAGGAG 1 cut(s) 171
BseXI GCAGC 5 cut(s) 499, 602, 1128, 1180, 1246
BseYI CCCAGC 1 cut(s) 209
BshFI GGCC 2 cut(s) 14, 178
BshNI GGYRCC 1 cut(s) 826
BshVI ATCGAT 2 cut(s) 171, 936
BsiHKAI GWGCWC 2 cut(s) 25, 1623
BsiSI CCGG 2 cut(s) 185, 707
BslFI GGGAC 2 cut(s) 192, 501
BslI CCNNNNNNNGG 3 cut(s) 752, 1691, 1697
BsmAI GTCTC 2 cut(s) 1349, 1700
BsmFI GGGAC 2 cut(s) 192, 501
BsnI GGCC 2 cut(s) 14, 178
Bso31I GGTCTC 2 cut(s) 1349, 1700
Bsp119I TTCGAA 1 cut(s) 624
Bsp1286I GDGCHC 3 cut(s) 25, 96, 1623
Bsp19I CCATGG 1 cut(s) 1273
BspACI CCGC 3 cut(s) 48, 617, 1050
BspANI GGCC 2 cut(s) 14, 178
BspCNI CTCAG 6 cut(s) 56, 409, 595, 625, 1120, 1144
BspDI ATCGAT 2 cut(s) 171, 936
BspHI TCATGA 4 cut(s) 118, 1483, 1545, 1623
BspLI GGNNCC 2 cut(s) 757, 828
BspMAI CTGCAG 2 cut(s) 571, 592
BspPI GGATC 3 cut(s) 750, 763, 1588
BspT104I TTCGAA 1 cut(s) 624
BspT107I GGYRCC 1 cut(s) 826
BspTNI GGTCTC 2 cut(s) 1349, 1700
BsrDI GCAATG 3 cut(s) 759, 1269, 1303
BsrI ACTGG 3 cut(s) 1309, 1345, 1417
BssECI CCNNGG 3 cut(s) 1073, 1074, 1273
BssNAI GTATAC 1 cut(s) 1069
BssSI CACGAG 1 cut(s) 1389
BssT1I CCWWGG 1 cut(s) 1273
Bst1107I GTATAC 1 cut(s) 1069
Bst2BI CACGAG 1 cut(s) 1389
Bst2UI CCWGG 2 cut(s) 1075, 1692
Bst4CI ACNGT 9 cut(s) 10, 134, 288, 448, 523, 826, 1087, 1093, 1429
Bst6I CTCTTC 4 cut(s) 103, 108, 346, 839
BstBAI YACGTR 1 cut(s) 722
BstBI TTCGAA 1 cut(s) 624
BstC8I GCNNGC 3 cut(s) 71, 888, 1713
BstDEI CTNAG 7 cut(s) 43, 417, 582, 633, 1128, 1152, 1204
BstDSI CCRYGG 1 cut(s) 1273
BstF5I GGATG 5 cut(s) 691, 1197, 1298, 1449, 1464
BstHHI GCGC 2 cut(s) 1141, 1368
BstMAI GTCTC 2 cut(s) 1349, 1700
BstMWI GCNNNNNNNGC 4 cut(s) 20, 66, 731, 1293
BstNI CCWGG 2 cut(s) 1075, 1692
BstNSI RCATGY 2 cut(s) 913, 1439
BstSCI CCNGG 3 cut(s) 706, 1073, 1690
BstSFI CTRYAG 3 cut(s) 567, 588, 1092
BstV1I GCAGC 5 cut(s) 499, 602, 1128, 1180, 1246
BstV2I GAAGAC 1 cut(s) 1532
BstX2I RGATCY 2 cut(s) 755, 1593
BstXI CCANNNNNNTGG 1 cut(s) 1280
BstYI RGATCY 2 cut(s) 755, 1593
BstZ17I GTATAC 1 cut(s) 1069
Bsu15I ATCGAT 2 cut(s) 171, 936
BsuRI GGCC 2 cut(s) 14, 178
BsuTUI ATCGAT 2 cut(s) 171, 936
BtgI CCRYGG 1 cut(s) 1273
BtsCI GGATG 5 cut(s) 691, 1197, 1298, 1449, 1464
BtsIMutI CAGTG 4 cut(s) 15, 519, 1083, 1620
Cac8I GCNNGC 3 cut(s) 71, 888, 1713
CciI TCATGA 4 cut(s) 118, 1483, 1545, 1623
CfoI GCGC 2 cut(s) 1141, 1368
Cfr13I GGNCC 1 cut(s) 176
ClaI ATCGAT 2 cut(s) 171, 936
Csp6I GTAC 1 cut(s) 1116
CviQI GTAC 1 cut(s) 1116
DdeI CTNAG 7 cut(s) 43, 417, 582, 633, 1128, 1152, 1204
Eam1104I CTCTTC 4 cut(s) 103, 108, 346, 839
EarI CTCTTC 4 cut(s) 103, 108, 346, 839
Ecl136II GAGCTC 1 cut(s) 1621
Eco130I CCWWGG 1 cut(s) 1273
Eco24I GRGCYC 2 cut(s) 96, 1623
Eco31I GGTCTC 2 cut(s) 1349, 1700
Eco53kI GAGCTC 1 cut(s) 1621
Eco57I CTGAAG 2 cut(s) 492, 1479
EcoICRI GAGCTC 1 cut(s) 1621
EcoRI GAATTC 2 cut(s) 428, 1780
EcoRII CCWGG 2 cut(s) 1073, 1690
EcoT14I CCWWGG 1 cut(s) 1273
EcoT38I GRGCYC 2 cut(s) 96, 1623
ErhI CCWWGG 1 cut(s) 1273
FaqI GGGAC 2 cut(s) 192, 501
FbaI TGATCA 4 cut(s) 481, 834, 904, 1480
FblI GTMKAC 1 cut(s) 1068
Fnu4HI GCNGC 7 cut(s) 48, 488, 591, 617, 1142, 1194, 1260
FokI GGATG 5 cut(s) 698, 1184, 1305, 1456, 1471
FriOI GRGCYC 2 cut(s) 96, 1623
Fsp4HI GCNGC 7 cut(s) 48, 488, 591, 617, 1142, 1194, 1260
FspBI CTAG 4 cut(s) 504, 974, 1419, 1598
FspI TGCGCA 1 cut(s) 1367
GlaI GCGC 2 cut(s) 1140, 1367
GluI GCNGC 7 cut(s) 48, 488, 591, 617, 1142, 1194, 1260
GsaI CCCAGC 1 cut(s) 213
HaeIII GGCC 2 cut(s) 14, 178
HapII CCGG 2 cut(s) 185, 707
HhaI GCGC 2 cut(s) 1141, 1368
Hin6I GCGC 2 cut(s) 1139, 1366
HinP1I GCGC 2 cut(s) 1139, 1366
HincII GTYRAC 1 cut(s) 1081
HindII GTYRAC 1 cut(s) 1081
HindIII AAGCTT 1 cut(s) 67
HpaII CCGG 2 cut(s) 185, 707
HphI GGTGA 2 cut(s) 1070, 1140
Hpy166II GTNNAC 3 cut(s) 1069, 1081, 1386
Hpy188I TCNGA 9 cut(s) 238, 403, 444, 634, 672, 755, 853, 1674, 1700
Hpy8I GTNNAC 3 cut(s) 1069, 1081, 1386
HpyAV CCTTC 4 cut(s) 61, 71, 543, 547
HpyCH4III ACNGT 9 cut(s) 10, 134, 288, 448, 523, 826, 1087, 1093, 1429
HpyCH4IV ACGT 2 cut(s) 533, 721
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 66, 731, 1293
HpyF3I CTNAG 7 cut(s) 43, 417, 582, 633, 1128, 1152, 1204
HpySE526I ACGT 2 cut(s) 533, 721
HspAI GCGC 2 cut(s) 1139, 1366
Ksp22I TGATCA 4 cut(s) 481, 834, 904, 1480
LmnI GCTCC 3 cut(s) 78, 1002, 1618
Lsp1109I GCAGC 5 cut(s) 499, 602, 1128, 1180, 1246
LweI GCATC 4 cut(s) 124, 1015, 1283, 1698
MaeI CTAG 4 cut(s) 504, 974, 1419, 1598
MaeII ACGT 2 cut(s) 533, 721
MaeIII GTNAC 4 cut(s) 809, 1058, 1087, 1336
MfeI CAATTG 3 cut(s) 1045, 1464, 1760
MflI RGATCY 2 cut(s) 755, 1593
MhlI GDGCHC 3 cut(s) 25, 96, 1623
MlyI GAGTC 6 cut(s) 35, 214, 428, 644, 973, 1055
MmeI TCCRAC 2 cut(s) 381, 733
MseI TTAA 7 cut(s) 159, 198, 651, 660, 693, 917, 969
MslI CAYNNNNRTG 1 cut(s) 807
MspI CCGG 2 cut(s) 185, 707
MspR9I CCNGG 3 cut(s) 708, 1075, 1692
MunI CAATTG 3 cut(s) 1045, 1464, 1760
MvaI CCWGG 2 cut(s) 1075, 1692
MwoI GCNNNNNNNGC 4 cut(s) 20, 66, 731, 1293
NciI CCSGG 1 cut(s) 708
NcoI CCATGG 1 cut(s) 1273
NlaIV GGNNCC 2 cut(s) 757, 828
NmuCI GTSAC 2 cut(s) 809, 1058
NsbI TGCGCA 1 cut(s) 1367
NspI RCATGY 2 cut(s) 913, 1439
NspV TTCGAA 1 cut(s) 624
PagI TCATGA 4 cut(s) 118, 1483, 1545, 1623
PasI CCCWGGG 1 cut(s) 1074
PciI ACATGT 2 cut(s) 909, 1435
PfeI GAWTC 6 cut(s) 361, 714, 1173, 1208, 1490, 1630
PfoI TCCNGGA 1 cut(s) 706
PkrI GCNGC 7 cut(s) 49, 489, 592, 618, 1143, 1195, 1261
PleI GAGTC 6 cut(s) 35, 213, 427, 643, 972, 1055
PpsI GAGTC 6 cut(s) 35, 213, 427, 643, 972, 1055
Ppu21I YACGTR 1 cut(s) 722
PscI ACATGT 2 cut(s) 909, 1435
Psp124BI GAGCTC 1 cut(s) 1623
Psp1406I AACGTT 1 cut(s) 533
Psp6I CCWGG 2 cut(s) 1073, 1690
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 2 cut(s) 1073, 1690
PspN4I GGNNCC 2 cut(s) 757, 828
PspPI GGNCC 1 cut(s) 176
PstI CTGCAG 2 cut(s) 571, 592
PsuI RGATCY 2 cut(s) 755, 1593
RsaI GTAC 1 cut(s) 1117
RsaNI GTAC 1 cut(s) 1116
RseI CAYNNNNRTG 1 cut(s) 807
SacI GAGCTC 1 cut(s) 1623
SaqAI TTAA 7 cut(s) 159, 198, 651, 660, 693, 917, 969
SatI GCNGC 7 cut(s) 48, 488, 591, 617, 1142, 1194, 1260
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 6 cut(s) 35, 214, 428, 644, 973, 1055
ScrFI CCNGG 3 cut(s) 708, 1075, 1692
SduI GDGCHC 3 cut(s) 25, 96, 1623
SfaNI GCATC 4 cut(s) 124, 1015, 1283, 1698
SfcI CTRYAG 3 cut(s) 567, 588, 1092
SfuI TTCGAA 1 cut(s) 624
SmiMI CAYNNNNRTG 1 cut(s) 807
SmlI CTYRAG 2 cut(s) 735, 1219
SmoI CTYRAG 2 cut(s) 735, 1219
SpeI ACTAGT 1 cut(s) 973
SsiI CCGC 3 cut(s) 48, 617, 1050
SspI AATATT 1 cut(s) 1514
SspMI CTAG 4 cut(s) 504, 974, 1419, 1598
SstI GAGCTC 1 cut(s) 1623
StyD4I CCNGG 3 cut(s) 706, 1073, 1690
StyI CCWWGG 1 cut(s) 1273
TaaI ACNGT 9 cut(s) 10, 134, 288, 448, 523, 826, 1087, 1093, 1429
TaiI ACGT 2 cut(s) 536, 724
TaqI TCGA 5 cut(s) 171, 316, 624, 936, 1752
TaqII GACCGA 1 cut(s) 216
TatI WGTACW 1 cut(s) 1115
TauI GCSGC 2 cut(s) 50, 619
TfiI GAWTC 6 cut(s) 361, 714, 1173, 1208, 1490, 1630
Tru1I TTAA 7 cut(s) 159, 198, 651, 660, 693, 917, 969
Tru9I TTAA 7 cut(s) 159, 198, 651, 660, 693, 917, 969
TscAI CASTG 4 cut(s) 15, 526, 1090, 1620
TseFI GTSAC 2 cut(s) 809, 1058
TseI GCWGC 5 cut(s) 487, 590, 1141, 1193, 1259
Tsp45I GTSAC 2 cut(s) 809, 1058
TspDTI ATGAA 9 cut(s) 95, 315, 333, 527, 585, 702, 810, 857, 1518
TspRI CASTG 4 cut(s) 15, 526, 1090, 1620
XapI RAATTY 6 cut(s) 428, 627, 1038, 1133, 1769, 1780
XbaI TCTAGA 1 cut(s) 1418
XceI RCATGY 2 cut(s) 913, 1439
XmiI GTMKAC 1 cut(s) 1068
XspI CTAG 4 cut(s) 504, 974, 1419, 1598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.