Rh5DG136800

Protein prune homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006920
Physical Location & Seq
Reverse (-)
13718307 .. 13723457
5151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG136800.1

Sequence Viewer

Length: 1563 bp
ATGTCAGATATTGGTCATAAACAGCCACAAGATACCTTATTAGATGTGTTTGAACGGTTCAGTCCAAATGAAAAACCCTTTGATCGAATTACTCCAAAGGAAAAATCCTCTTCATTCCGTATAAAGAAGAACCCTTTTGAGTTTAGTCCAGAAAAGAACTTTGAGTGGTCAGAGAATGGTAAATCTGAGTCCTTTGAATTCACAAATGTTCAGACTGTGTTTTGCTCTAACTTGGCTACTGAAGAAAATGATCAGCAGCGTGAGAGTGTATCTAGTAATACAAGTCCCACTGTTGAAAAAACATTTCGTGTGAATAGAAGGAAGGAAAACCTGTCTGCAGTTCCACTTCCTCAGTCTGCAGCTTCATTTTACAATGGCAACTCGCCGCAGTTCGAAATTTCTGAGTCATGTCCAAGCATTAAGAGGCTTAATCGGTATCTGAAAGCCACAAAGGATGAAGTTAATGCTGGTGTTCCGGGAAGATTTTTACATGCTGTAATAGCTCAAGATGTTCCTGATGTCGGATCCCTTGCCTCAACCATCATGTATGCTTTCTACGTGAATGAAACACATAAAAGTGACCATTTATGCACGGTGCCTGTGATCAACATGAAGAGGTCAGACATAAGTTCTCAAAGCGAACTCAAATGGTTGCTTGCTTCTTGTCAAATTGATCACATGTCCTTAATTTTTGTAGATGAGATCGATCTGTCATATTATGATCTATTTGGGAGTCTTAAACTAGTTCTACTAAATGGCTACAAGCTCCCAACCAAGCAAGAGGCATTGAAAGATGCAGTAGTTGAAATTTTCAATTGCGGAAAGGGTGACTCTGTATACCCCTGGGTTGACACTGTTACTGTAGGAGAGGACAGCTCATGCTGTACTCCTATCGCTGAGAAATTTGCGCTGCGCTCACCTGAGATTTTGGCTGGACATGGATTCAGTAGATTTTTGTTAGCTGCCATCCTCTTAGATTCTGCAAACTTGAGCAGTTCTAATTGTTCCATCAAAGATAAATACATGGCTGCAATGCTAATCCATGGTGCTGGTCGCTTTGGATGCGATGGTCTTTACCAGTTATTGAGATACAAGATGTATGATGTAACTGGTCTCAAAGTAGCTGATATATTGCGCAAGGATTTCAAAAAGTGGACACGAGTAGGTAATCAGGACAATACCGGTTCTAGATTGACAGTATCAGATGTTGGGATGAGCTCTATTGGGACGTCAATTGCAGAACTTCTTGATCATGAGGAATCCTCGGTCCATGAAATCAAATATTTTCAACAGGTGGAGAAACTTCGTCTTCTCATGATTGTTTCTGGGTATTATGATTTGAAAAAGAACTTCAAGAGAGAGATCCTAGTGTCTGCTCAATCAGTGGAGCTCATGAGGAATCTGCTGTCTTTCTTCAATTCAAGTGCTTCCCAACTACCTCTGAAAGCTATCCCCAAGCCAGGTCTCGGAGAGCAGATGCAAGCATTTGAGATTGACAAGATCATATCAAGAAAGACCATCGAGCGGCTCTTGGAAGAATTTGGTGGGAATTCAAAAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
DHHA2

Protein Analysis

520

Amino Acids

58.42

Weight (kDa)

6.21

Isoelectric Point (pI)

51.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHA2 PF02833 366 - 505 6.3e-10 DHHA2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1232
Acc16I TGCGCA 1 cut(s) 1136
AccB1I GGYRCC 1 cut(s) 595
AccBSI CCGCTC 1 cut(s) 1525
AccI GTMKAC 1 cut(s) 837
AciI CCGC 3 cut(s) 386, 819, 1525
AclWI GGATC 3 cut(s) 519, 532, 1357
AcsI RAATTY 6 cut(s) 197, 396, 807, 902, 1538, 1549
AcuI CTGAAG 1 cut(s) 261
AcyI GRCGYC 1 cut(s) 1229
AfaI GTAC 1 cut(s) 886
AfiI CCNNNNNNNGG 4 cut(s) 521, 1460, 1466, 1524
AflIII ACRYGT 1 cut(s) 678
AgeI ACCGGT 1 cut(s) 1181
AhlI ACTAGT 1 cut(s) 742
AjnI CCWGG 2 cut(s) 842, 1459
AluBI AGCT 9 cut(s) 362, 503, 766, 876, 962, 1124, 1218, 1390, 1448
AluI AGCT 9 cut(s) 362, 503, 766, 876, 962, 1124, 1218, 1390, 1448
Alw21I GWGCWC 2 cut(s) 1220, 1392
Alw26I GTCTC 2 cut(s) 1118, 1469
AlwI GGATC 3 cut(s) 519, 532, 1357
ApeKI GCWGC 5 cut(s) 256, 359, 910, 962, 1028
ApoI RAATTY 6 cut(s) 197, 396, 807, 902, 1538, 1549
AsiGI ACCGGT 1 cut(s) 1181
AspLEI GCGC 3 cut(s) 910, 915, 1137
AspS9I GGNCC 1 cut(s) 1267
AsuC2I CCSGG 1 cut(s) 477
AsuHPI GGTGA 2 cut(s) 839, 909
AsuII TTCGAA 1 cut(s) 393
AvaII GGWCC 1 cut(s) 1267
BamHI GGATCC 1 cut(s) 524
BanI GGYRCC 1 cut(s) 595
BanII GRGCYC 2 cut(s) 1220, 1392
BauI CACGAG 1 cut(s) 1158
BbsI GAAGAC 1 cut(s) 1301
Bbv12I GWGCWC 2 cut(s) 1220, 1392
BbvI GCAGC 5 cut(s) 268, 371, 897, 949, 1015
BccI CCATC 5 cut(s) 548, 974, 1016, 1061, 1526
BciT130I CCWGG 2 cut(s) 844, 1461
BclI TGATCA 4 cut(s) 250, 603, 673, 1249
BcnI CCSGG 1 cut(s) 477
BcoDI GTCTC 2 cut(s) 1118, 1469
BcuI ACTAGT 1 cut(s) 742
BfaI CTAG 4 cut(s) 273, 743, 1188, 1367
BfmI CTRYAG 3 cut(s) 336, 357, 861
BisI GCNGC 7 cut(s) 257, 360, 386, 911, 963, 1029, 1526
BlsI GCNGC 7 cut(s) 258, 361, 387, 912, 964, 1030, 1527
Bme1390I CCNGG 3 cut(s) 477, 844, 1461
Bme18I GGWCC 1 cut(s) 1267
BmgT120I GGNCC 1 cut(s) 1267
BmiI GGNNCC 2 cut(s) 526, 597
BmrFI CCNGG 3 cut(s) 477, 844, 1461
BmsI GCATC 3 cut(s) 784, 1052, 1467
BpiI GAAGAC 1 cut(s) 1301
BplI GAGNNNNNCTC 4 cut(s) 860, 892, 1247, 1279
Bpu14I TTCGAA 1 cut(s) 393
BpuEI CTTGAG 2 cut(s) 489, 1009
BpuMI CCSGG 1 cut(s) 477
Bsa29I ATCGAT 1 cut(s) 705
BsaAI YACGTR 1 cut(s) 559
BsaHI GRCGYC 1 cut(s) 1229
BsaI GGTCTC 2 cut(s) 1118, 1469
BsaJI CCNNGG 4 cut(s) 842, 843, 1042, 1263
BsaWI WCCGGW 1 cut(s) 1181
Bsc4I CCNNNNNNNGG 4 cut(s) 521, 1460, 1466, 1524
Bse118I RCCGGY 1 cut(s) 1181
Bse1I ACTGG 2 cut(s) 1078, 1114
Bse3DI GCAATG 1 cut(s) 1038
BseBI CCWGG 2 cut(s) 844, 1461
BseCI ATCGAT 1 cut(s) 705
BseDI CCNNGG 4 cut(s) 842, 843, 1042, 1263
BseGI GGATG 4 cut(s) 460, 966, 1067, 1218
BseLI CCNNNNNNNGG 4 cut(s) 521, 1460, 1466, 1524
BseMI GCAATG 1 cut(s) 1038
BseMII CTCAG 5 cut(s) 177, 365, 393, 888, 912
BseNI ACTGG 2 cut(s) 1078, 1114
BseXI GCAGC 5 cut(s) 268, 371, 897, 949, 1015
BshNI GGYRCC 1 cut(s) 595
BshTI ACCGGT 1 cut(s) 1181
BshVI ATCGAT 1 cut(s) 705
BsiHKAI GWGCWC 2 cut(s) 1220, 1392
BsiSI CCGG 2 cut(s) 476, 1182
BslFI GGGAC 2 cut(s) 270, 1240
BslI CCNNNNNNNGG 4 cut(s) 521, 1460, 1466, 1524
BsmAI GTCTC 2 cut(s) 1118, 1469
BsmFI GGGAC 2 cut(s) 270, 1240
Bso31I GGTCTC 2 cut(s) 1118, 1469
Bsp119I TTCGAA 1 cut(s) 393
Bsp1286I GDGCHC 2 cut(s) 1220, 1392
Bsp19I CCATGG 1 cut(s) 1042
BspACI CCGC 3 cut(s) 386, 819, 1525
BspCNI CTCAG 5 cut(s) 178, 364, 394, 889, 913
BspDI ATCGAT 1 cut(s) 705
BspHI TCATGA 3 cut(s) 1252, 1314, 1392
BspLI GGNNCC 2 cut(s) 526, 597
BspMAI CTGCAG 2 cut(s) 340, 361
BspPI GGATC 3 cut(s) 519, 532, 1357
BspT104I TTCGAA 1 cut(s) 393
BspT107I GGYRCC 1 cut(s) 595
BspTNI GGTCTC 2 cut(s) 1118, 1469
BsrBI CCGCTC 1 cut(s) 1525
BsrDI GCAATG 1 cut(s) 1038
BsrFI RCCGGY 1 cut(s) 1181
BsrI ACTGG 2 cut(s) 1078, 1114
BssAI RCCGGY 1 cut(s) 1181
BssECI CCNNGG 4 cut(s) 842, 843, 1042, 1263
BssNAI GTATAC 1 cut(s) 838
BssNI GRCGYC 1 cut(s) 1229
BssSI CACGAG 1 cut(s) 1158
BssT1I CCWWGG 1 cut(s) 1042
Bst1107I GTATAC 1 cut(s) 838
Bst2BI CACGAG 1 cut(s) 1158
Bst2UI CCWGG 2 cut(s) 844, 1461
Bst4CI ACNGT 7 cut(s) 57, 217, 292, 595, 856, 862, 1198
Bst6I CTCTTC 2 cut(s) 115, 608
BstACI GRCGYC 1 cut(s) 1229
BstBAI YACGTR 1 cut(s) 559
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 2 cut(s) 657, 1482
BstDEI CTNAG 6 cut(s) 186, 351, 402, 897, 921, 973
BstDSI CCRYGG 1 cut(s) 1042
BstF5I GGATG 4 cut(s) 460, 966, 1067, 1218
BstHHI GCGC 3 cut(s) 910, 915, 1137
BstMAI GTCTC 2 cut(s) 1118, 1469
BstMWI GCNNNNNNNGC 2 cut(s) 500, 1062
BstNI CCWGG 2 cut(s) 844, 1461
BstNSI RCATGY 2 cut(s) 494, 682
BstSCI CCNGG 3 cut(s) 475, 842, 1459
BstSFI CTRYAG 3 cut(s) 336, 357, 861
BstV1I GCAGC 5 cut(s) 268, 371, 897, 949, 1015
BstV2I GAAGAC 1 cut(s) 1301
BstX2I RGATCY 2 cut(s) 524, 1362
BstXI CCANNNNNNTGG 1 cut(s) 1049
BstYI RGATCY 2 cut(s) 524, 1362
BstZ17I GTATAC 1 cut(s) 838
Bsu15I ATCGAT 1 cut(s) 705
BsuTUI ATCGAT 1 cut(s) 705
BtgI CCRYGG 1 cut(s) 1042
BtgZI GCGATG 1 cut(s) 1080
BtsCI GGATG 4 cut(s) 460, 966, 1067, 1218
BtsIMutI CAGTG 3 cut(s) 288, 852, 1389
Cac8I GCNNGC 2 cut(s) 657, 1482
CciI TCATGA 3 cut(s) 1252, 1314, 1392
CfoI GCGC 3 cut(s) 910, 915, 1137
Cfr10I RCCGGY 1 cut(s) 1181
Cfr13I GGNCC 1 cut(s) 1267
ClaI ATCGAT 1 cut(s) 705
Csp6I GTAC 1 cut(s) 885
CspAI ACCGGT 1 cut(s) 1181
CviQI GTAC 1 cut(s) 885
DdeI CTNAG 6 cut(s) 186, 351, 402, 897, 921, 973
Eam1104I CTCTTC 2 cut(s) 115, 608
EarI CTCTTC 2 cut(s) 115, 608
Ecl136II GAGCTC 2 cut(s) 1218, 1390
Eco130I CCWWGG 1 cut(s) 1042
Eco24I GRGCYC 2 cut(s) 1220, 1392
Eco31I GGTCTC 2 cut(s) 1118, 1469
Eco47I GGWCC 1 cut(s) 1267
Eco53kI GAGCTC 2 cut(s) 1218, 1390
Eco57I CTGAAG 1 cut(s) 261
EcoICRI GAGCTC 2 cut(s) 1218, 1390
EcoRI GAATTC 2 cut(s) 197, 1549
EcoRII CCWGG 2 cut(s) 842, 1459
EcoT14I CCWWGG 1 cut(s) 1042
EcoT38I GRGCYC 2 cut(s) 1220, 1392
ErhI CCWWGG 1 cut(s) 1042
FaqI GGGAC 2 cut(s) 270, 1240
FbaI TGATCA 4 cut(s) 250, 603, 673, 1249
FblI GTMKAC 1 cut(s) 837
Fnu4HI GCNGC 7 cut(s) 257, 360, 386, 911, 963, 1029, 1526
FokI GGATG 4 cut(s) 467, 953, 1074, 1225
FriOI GRGCYC 2 cut(s) 1220, 1392
Fsp4HI GCNGC 7 cut(s) 257, 360, 386, 911, 963, 1029, 1526
FspBI CTAG 4 cut(s) 273, 743, 1188, 1367
FspI TGCGCA 1 cut(s) 1136
GlaI GCGC 3 cut(s) 909, 914, 1136
GluI GCNGC 7 cut(s) 257, 360, 386, 911, 963, 1029, 1526
HapII CCGG 2 cut(s) 476, 1182
HhaI GCGC 3 cut(s) 910, 915, 1137
Hin1I GRCGYC 1 cut(s) 1229
Hin6I GCGC 3 cut(s) 908, 913, 1135
HinP1I GCGC 3 cut(s) 908, 913, 1135
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HinfI GANTC 8 cut(s) 188, 404, 733, 830, 942, 977, 1259, 1399
HpaII CCGG 2 cut(s) 476, 1182
HphI GGTGA 2 cut(s) 839, 909
Hpy166II GTNNAC 3 cut(s) 838, 850, 1155
Hpy8I GTNNAC 3 cut(s) 838, 850, 1155
HpyAV CCTTC 2 cut(s) 312, 316
HpyCH4III ACNGT 7 cut(s) 57, 217, 292, 595, 856, 862, 1198
HpyCH4IV ACGT 2 cut(s) 558, 1229
HpyCH4V TGCA 8 cut(s) 338, 359, 591, 797, 983, 1031, 1238, 1480
HpyF10VI GCNNNNNNNGC 2 cut(s) 500, 1062
HpyF3I CTNAG 6 cut(s) 186, 351, 402, 897, 921, 973
HpySE526I ACGT 2 cut(s) 558, 1229
Hsp92I GRCGYC 1 cut(s) 1229
HspAI GCGC 3 cut(s) 908, 913, 1135
Ksp22I TGATCA 4 cut(s) 250, 603, 673, 1249
LmnI GCTCC 2 cut(s) 771, 1387
Lsp1109I GCAGC 5 cut(s) 268, 371, 897, 949, 1015
LweI GCATC 3 cut(s) 784, 1052, 1467
MaeI CTAG 4 cut(s) 273, 743, 1188, 1367
MaeII ACGT 2 cut(s) 558, 1229
MaeIII GTNAC 4 cut(s) 578, 827, 856, 1105
MbiI CCGCTC 1 cut(s) 1525
MboII GAAGA 8 cut(s) 102, 139, 254, 492, 625, 1301, 1405, 1547
MfeI CAATTG 2 cut(s) 814, 1233
MflI RGATCY 2 cut(s) 524, 1362
MhlI GDGCHC 2 cut(s) 1220, 1392
MlyI GAGTC 4 cut(s) 197, 413, 742, 824
MmeI TCCRAC 1 cut(s) 502
MseI TTAA 5 cut(s) 420, 429, 462, 686, 738
MslI CAYNNNNRTG 1 cut(s) 576
MspI CCGG 2 cut(s) 476, 1182
MspR9I CCNGG 3 cut(s) 477, 844, 1461
MteI GCGCNGCGC 1 cut(s) 911
MunI CAATTG 2 cut(s) 814, 1233
MvaI CCWGG 2 cut(s) 844, 1461
MwoI GCNNNNNNNGC 2 cut(s) 500, 1062
NciI CCSGG 1 cut(s) 477
NcoI CCATGG 1 cut(s) 1042
NlaIV GGNNCC 2 cut(s) 526, 597
NmuCI GTSAC 2 cut(s) 578, 827
NsbI TGCGCA 1 cut(s) 1136
NspI RCATGY 2 cut(s) 494, 682
NspV TTCGAA 1 cut(s) 393
PagI TCATGA 3 cut(s) 1252, 1314, 1392
PasI CCCWGGG 1 cut(s) 843
PciI ACATGT 1 cut(s) 678
PfeI GAWTC 4 cut(s) 942, 977, 1259, 1399
PfoI TCCNGGA 1 cut(s) 475
PinAI ACCGGT 1 cut(s) 1181
PkrI GCNGC 7 cut(s) 258, 361, 387, 912, 964, 1030, 1527
PleI GAGTC 4 cut(s) 196, 412, 741, 824
PpsI GAGTC 4 cut(s) 196, 412, 741, 824
Ppu21I YACGTR 1 cut(s) 559
PscI ACATGT 1 cut(s) 678
Psp124BI GAGCTC 2 cut(s) 1220, 1392
Psp6I CCWGG 2 cut(s) 842, 1459
PspGI CCWGG 2 cut(s) 842, 1459
PspN4I GGNNCC 2 cut(s) 526, 597
PspPI GGNCC 1 cut(s) 1267
PstI CTGCAG 2 cut(s) 340, 361
PsuI RGATCY 2 cut(s) 524, 1362
RsaI GTAC 1 cut(s) 886
RsaNI GTAC 1 cut(s) 885
RseI CAYNNNNRTG 1 cut(s) 576
SacI GAGCTC 2 cut(s) 1220, 1392
SaqAI TTAA 5 cut(s) 420, 429, 462, 686, 738
SatI GCNGC 7 cut(s) 257, 360, 386, 911, 963, 1029, 1526
Sau96I GGNCC 1 cut(s) 1267
SchI GAGTC 4 cut(s) 197, 413, 742, 824
ScrFI CCNGG 3 cut(s) 477, 844, 1461
SduI GDGCHC 2 cut(s) 1220, 1392
SfaNI GCATC 3 cut(s) 784, 1052, 1467
SfcI CTRYAG 3 cut(s) 336, 357, 861
SfuI TTCGAA 1 cut(s) 393
SinI GGWCC 1 cut(s) 1267
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 2 cut(s) 504, 988
SmoI CTYRAG 2 cut(s) 504, 988
SpeI ACTAGT 1 cut(s) 742
SsiI CCGC 3 cut(s) 386, 819, 1525
SspI AATATT 1 cut(s) 1283
SspMI CTAG 4 cut(s) 273, 743, 1188, 1367
SstI GAGCTC 2 cut(s) 1220, 1392
StyD4I CCNGG 3 cut(s) 475, 842, 1459
StyI CCWWGG 1 cut(s) 1042
TaaI ACNGT 7 cut(s) 57, 217, 292, 595, 856, 862, 1198
TaiI ACGT 2 cut(s) 561, 1232
TaqI TCGA 4 cut(s) 85, 393, 705, 1521
TaqII GACCGA 1 cut(s) 1255
TatI WGTACW 1 cut(s) 884
TauI GCSGC 2 cut(s) 388, 1528
TfiI GAWTC 4 cut(s) 942, 977, 1259, 1399
Tru1I TTAA 5 cut(s) 420, 429, 462, 686, 738
Tru9I TTAA 5 cut(s) 420, 429, 462, 686, 738
TscAI CASTG 3 cut(s) 295, 859, 1389
TseFI GTSAC 2 cut(s) 578, 827
TseI GCWGC 5 cut(s) 256, 359, 910, 962, 1028
Tsp45I GTSAC 2 cut(s) 578, 827
TspDTI ATGAA 7 cut(s) 84, 102, 354, 471, 579, 626, 1287
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 3 cut(s) 295, 859, 1389
VpaK11BI GGWCC 1 cut(s) 1267
XapI RAATTY 6 cut(s) 197, 396, 807, 902, 1538, 1549
XbaI TCTAGA 1 cut(s) 1187
XceI RCATGY 2 cut(s) 494, 682
XmiI GTMKAC 1 cut(s) 837
XspI CTAG 4 cut(s) 273, 743, 1188, 1367
ZraI GACGTC 1 cut(s) 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.