Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005866

Physical Location & Seq

Genomic Coordinates Reverse (-)

35877339 .. 35884323

Gene Structure 6985 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1265500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3021 bp

ATGGCGGTCAGACTAACGACCTGGTTCGTCACCCAGGGGCTCAGCAGTACCGGCTCCAACTCCACCTCCCGCCACCGCTCATATTCAAACGCTCGCTTTTCGTTTCACTGCTCTCCTGCTTCTTCCTGCAAGCCCCGGCACCGTAATCTCAGTTGCAATTGCGTGAATAAGAGGCATAGACTAAGGAAGAAAGGCTCGGTTGAACAGCCTTCGACGACTACAGATTTCCGGTTCAATGGCAACGACGACTCCGAATCTGACGGTGCTTCTTCGGCGGGCGTCATCGTCCCCATTGAAAGCGTATCTGATCACGAAGCTCCCACTGCCAATGGAAACGATTCGATTTTGACAACTTTAACTCCGTCTGATCAAGCAAATCCTTCGGCGTATAATACTCAGGATTTGGTTGGCATGATTAGAAATGCAGAGAAAAATATCCATCTTCTCAACCGGGCGCGGGTTAATGCGCTTGAAGACCTTGACAAGATTCTCGGTGAGAAGGAGGCATTGCAAGGACAGATGAATGCTTTGGAGATGAGATTGGCAGAGACCGATGCGCGGATTAGAGTAGCTGCCCAGGAAAAAATAAAGGTGGAACTTTTGGAAAACCAGTTAGATAAGATGCGGAATGAACTGAATCTCAATGGTGACGGTGGTGTTGAAAGAGGTCAGGTGGAGATTTTTGAGAATGAAGACCAGCTGTTTAACGAAGAAGCTCCTCTACCATACAGAACTAGCATCAATGCTCTTGTTGCCAATCTGAATGCATTGAGATTGGAAAATCAATCCCTAAAGAATGATGTAGAAGCACTCAGGGAGGAGCTTAGCTATGTGAAGAATACGGATGAACGGGTGGTAATGCTGGAAAATCAACGGTCGACTTTAGAGTCTGCGTTGAAAGAGTTGGAATCGAAATTGTCAGTTTCTCAGGAAGAAGTGTCCAAACTATCCAATCTTAAAGTTGAATGCAAAGGATTATGGGAGAAGGTGGAGAATTTGCAGCTACTGCTAGATAAGTCAACCAAACAGGCAGATCAAGCTATTGTAGTTTTACAGCAGAACCAAGAGATTCAAAAGAAAGTTGATAAATTAGAAGAATCTCTTGAAACAGCTAATGTCTATAGAGAGTCATCAGAAAAGATGCAACGATATAATGAGCTGATGCAACAGAAAATAAAACTTATGGAGGACCGGCTTCAAAGATCTGACGAAGAGATACATTCTTATGTTCAATTATATCAAGAATCTGTGGAGGAATTTCAAGATACACTAAATACTCTAAAAGAAGAAAGCAAGAGAAGGGCAGTTGATGAACCTGTGGATGATATGCCATGGGAATTTTGGAGTCGTTTATTGCTTATGATCGATGGTTGGTTGTTTGAGAACAAAATATCAATTGATGATGCTCAGGTATTGAGAGAAATGGTTTGGAAGAGAGAGAGACGAATTCATGACTCATACATGGCCTGCAAGGAAAAGAACGAGCATGACGCTGTGTCTACATTTCTCAAACTGATATCATCACAAACAAGTCCAGGATTGCATGTCGTTCATATTGCAGCAGAGATGGCACCAGTTGCTAAGGTTGGTGGTTTGGGGGATGTTGTGGCTGGTCTTGGTAAAGCACTGCAGAAGAAGGGACACCTTGTGGAAATTGTTCTTCCCAAATATGACTGCATGCAGTACGATCGTGTTCCTGACCTAAGGGCCCTAGATCTGGTGTTAGAGTCATACTTTGACGGTCGACTATTCAAAAATAAAGTCTGGGTTGGCACTGTTGAAGGTCTTCCTGTTTATTTTATTGAGCCTCTGCATCCTGATAAGTTTTTCTGGAGAGGACGGTTTTATGGGGAACATGATGATTTCAAGCGATTTTCCTTTTTCAGTCGTGCAGCCCTTGAGTTGCTTCTTCAATCTGGCAAGCAACCAGACATAATTCACTGCCATGATTGGCAGACAGCTTTTGTTGCTCCACTTTACTGGGACCTATATGCGCCAAATGGATTGAACTCAGCTAGAATATGTTTTACATGCCACAACTTTGAATACCAAGGGACTGCACCTGCTTCAGAATTGGCATCTTGTGGTCTTGATGTCCACCAGCTAAATAGACCAGATAGAATGCAGGACAACTCAGCACATGATAGGATCAATGCTGTTAAGGGTGCAGTTGTATTCTCTAATATCGTGACAACAGTATCACCCACCTATGCACAAGAGGTGCTGACTGCTGAGGGAGGACACGGTCTCCATTCAACACTAAATTTTCATTCTAAGAAGTTCAATGGAGTTCTAAATGGTATTGATGCTGATGCATGGAATCCTGCCACTGATGCGTATCTCAAAGTACAGTACAGTGCTAATGATCGTCAGGGGAAAGCAGAAAATAAGGAAGCTTTAAGAAGAACTCTGGGGCTTTCTTCGGCAGATGTCAAGAGGCCATTGGTGGGCTGCATAACAAGATTGGTGCCACAGAAAGGTGTACATCTTATCAGACATGCGATATATCGAACATTGGAGCTCGGAGGACAGTTTGTACTTCTGGGTTCTAGCCCAAGCGAATTTGAGGGTCTTGCAAGCCATTTTGAGAATCATGATCATGTTCGTTTGATATTGAAGTACGACGACTCTCTTTCCCATGCCATTTTTGCTGCTTCTGATATGTTCATCATCCCGTCTATCTTTGAGCCTTGTGGACTTACACAGATGATAGCCATGAGATACGGTTCTATACCCATTGTAAGAAAAACTGGTGGTCTAAATGATAGTGTTTTTGATGTTGATGACGACACCGTACCTCTTCAGTTTCGGAATGGATATTCATTCTTGACTCCTAACGAACAGGGATTCAACGGGGCCTTGGGACGTGCATTCGCCCTCTACATGAATAAGCCGGATAGTTGGCAGCAGCTTGTTCAGAAGGTCATGAATATAGATTTCAGTTGGGATACTTCTGCATCACGGTACGAAGAACTCTACTCCAAGTCTCTGGCCAGAGCAAGGGCTGTCGCTCACGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009011 GO:0009058 GO:0009059 GO:0009250 GO:0009507 GO:0009536 GO:0009987 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026

Pfam Domains

Protein Families

Glyco_transf_5 Glycos_transf_1

Protein Analysis

1007

Amino Acids

113.68

Weight (kDa)

5.72

Isoelectric Point (pI)

40.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_5	PF08323	515 - 755	1.1e-66	Starch synthase catalytic domain
Glyco_transf_4	PF13439	529 - 741	3.5e-08	Glycosyltransferase Family 4
Glycos_transf_1	PF00534	810 - 926	5.5e-11	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	814 - 926	2.9e-08	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005866)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18240
fragaria_vesca	FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130
malus_domestica	MD05G1289600.v1.1 MD10G1265500.v1.1
prunus_persica	Prupe.4G076100_v2.0.a1
pyrus_communis	pycom05g26670 pycom10g22140
rosa_chinensis	RchiOBHm_Chr5g0013241
rosa_laevigata	RLG00000032000
rosa_multiflora	Rmu_co8500739.1_g000001 Rmu_sc0004521.1_g000017
rosa_roxburghii	Rroxscaffold_1G00063270
rosa_rugosa	Rorug05G0008200.1 Rorug05G0008300.1 Rorug05G0008400
rosa_samantha	Rh5AG103000 Rh5BG099800 Rh5CG111200 Rh5DG098600
rosa_wichuraiana	Rw5G008990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2071
AasI	GACNNNNNNGTC	1 cut(s)	886
Acc36I	ACCTGC	1 cut(s)	2071
AccB1I	GGYRCC	3 cut(s)	138, 1570, 2467
AccBSI	CCGCTC	1 cut(s)	78
AccI	GTMKAC	3 cut(s)	878, 1499, 1744
AccII	CGCG	2 cut(s)	457, 559
AciI	CCGC	7 cut(s)	5, 70, 76, 275, 457, 559, 625
AclWI	GGATC	1 cut(s)	2156
AcoI	YGGCCR	1 cut(s)	2991
AcsI	RAATTY	6 cut(s)	994, 1256, 1337, 1446, 2263, 2561
AcuI	CTGAAG	2 cut(s)	2052, 2786
AcyI	GRCGYC	1 cut(s)	279
AdeI	CACNNNGTG	1 cut(s)	1648
AfaI	GTAC	9 cut(s)	49, 1685, 2349, 2354, 2484, 2538, 2621, 2796, 2966
AfiI	CCNNNNNNNGG	6 cut(s)	457, 558, 1717, 2447, 2477, 3000
AhdI	GACNNNNNGTC	1 cut(s)	1495
AjiI	CACGTC	1 cut(s)	2867
AjnI	CCWGG	4 cut(s)	20, 33, 576, 1534
Alw21I	GWGCWC	1 cut(s)	2523
Alw26I	GTCTC	4 cut(s)	542, 1435, 2252, 2991
AlwI	GGATC	1 cut(s)	2156
AlwNI	CAGNNNCTG	1 cut(s)	1006
AoxI	GGCC	5 cut(s)	1464, 1707, 2438, 2856, 2991
ApaI	GGGCCC	1 cut(s)	1711
ApeKI	GCWGC	8 cut(s)	572, 1000, 1559, 1892, 2451, 2651, 2905, 2908
ApoI	RAATTY	6 cut(s)	994, 1256, 1337, 1446, 2263, 2561
ArsI	GACNNNNNNTTYG	2 cut(s)	1746, 1778
Asp700I	GAANNNNTTC	3 cut(s)	337, 1656, 1785
AspLEI	GCGC	4 cut(s)	457, 469, 559, 1996
AspS9I	GGNCC	5 cut(s)	1189, 1707, 1708, 1984, 2856
AsuC2I	CCSGG	2 cut(s)	136, 452
AsuHPI	GGTGA	4 cut(s)	22, 506, 659, 2193
AvaII	GGWCC	2 cut(s)	1189, 1984
AxyI	CCTNAGG	1 cut(s)	1703
BaeGI	GKGCMC	1 cut(s)	1711
BaeI	ACNNNNGTAYC	2 cut(s)	2181, 2214
BalI	TGGCCA	1 cut(s)	2993
BanI	GGYRCC	3 cut(s)	138, 1570, 2467
BanII	GRGCYC	3 cut(s)	42, 1711, 2523
BarI	GAAGNNNNNNTAC	2 cut(s)	705, 737
BbsI	GAAGAC	3 cut(s)	480, 699, 1778
Bbv12I	GWGCWC	1 cut(s)	2523
BbvCI	CCTCAGC	1 cut(s)	2232
BbvI	GCAGC	8 cut(s)	559, 1012, 1571, 1904, 2438, 2638, 2917, 2920
BccI	CCATC	3 cut(s)	447, 1361, 1561
BcgI	CGANNNNNNTGC	4 cut(s)	363, 397, 1670, 1704
BciT130I	CCWGG	4 cut(s)	22, 35, 578, 1536
BciVI	GTATCC	1 cut(s)	2941
BclI	TGATCA	3 cut(s)	307, 367, 2596
BcnI	CCSGG	2 cut(s)	136, 452
BcoDI	GTCTC	4 cut(s)	542, 1435, 2252, 2991
BfaI	CTAG	5 cut(s)	735, 1010, 1712, 2016, 2550
BfmI	CTRYAG	3 cut(s)	219, 1120, 1628
BfuAI	ACCTGC	1 cut(s)	2071
BfuI	GTATCC	1 cut(s)	2941
BglII	AGATCT	2 cut(s)	1202, 1714
BisI	GCNGC	8 cut(s)	573, 1001, 1560, 1893, 2452, 2652, 2906, 2909
BlpI	GCTNAGC	2 cut(s)	41, 824
BlsI	GCNGC	8 cut(s)	574, 1002, 1561, 1894, 2453, 2653, 2907, 2910
Bme1390I	CCNGG	6 cut(s)	22, 35, 136, 452, 578, 1536
Bme18I	GGWCC	2 cut(s)	1189, 1984
BmeRI	GACNNNNNGTC	1 cut(s)	1495
BmgBI	CACGTC	1 cut(s)	2867
BmgT120I	GGNCC	5 cut(s)	1189, 1707, 1708, 1984, 2856
BmiI	GGNNCC	7 cut(s)	55, 140, 1572, 1709, 1985, 2469, 2857
BmrFI	CCNGG	6 cut(s)	22, 35, 136, 452, 578, 1536
BmrI	ACTGGG	1 cut(s)	1990
BmuI	ACTGGG	1 cut(s)	1990
BpiI	GAAGAC	3 cut(s)	480, 699, 1778
BpmI	CTGGAG	1 cut(s)	1852
Bpu10I	CCTNAGC	3 cut(s)	1407, 1581, 2232
Bpu1102I	GCTNAGC	2 cut(s)	41, 824
BpuEI	CTTGAG	1 cut(s)	1919
BpuMI	CCSGG	2 cut(s)	136, 452
Bsa29I	ATCGAT	1 cut(s)	1365
BsaBI	GATNNNNATC	1 cut(s)	2337
BsaHI	GRCGYC	1 cut(s)	279
BsaI	GGTCTC	2 cut(s)	542, 2252
BsaJI	CCNNGG	7 cut(s)	33, 34, 134, 576, 1331, 2050, 2859
BsaWI	WCCGGW	1 cut(s)	228
BsaXI	ACNNNNNCTCC	8 cut(s)	50, 80, 233, 263, 343, 373, 2232, 2262
Bsc4I	CCNNNNNNNGG	6 cut(s)	457, 558, 1717, 2447, 2477, 3000
Bse118I	RCCGGY	2 cut(s)	50, 1191
Bse1I	ACTGG	4 cut(s)	610, 1574, 1985, 2755
Bse21I	CCTNAGG	1 cut(s)	1703
Bse3DI	GCAATG	1 cut(s)	506
Bse8I	GATNNNNATC	1 cut(s)	2337
BseBI	CCWGG	4 cut(s)	22, 35, 578, 1536
BseCI	ATCGAT	1 cut(s)	1365
BseDI	CCNNGG	7 cut(s)	33, 34, 134, 576, 1331, 2050, 2859
BseGI	GGATG	5 cut(s)	850, 1327, 1606, 1813, 2670
BseJI	GATNNNNATC	1 cut(s)	2337
BseLI	CCNNNNNNNGG	6 cut(s)	457, 558, 1717, 2447, 2477, 3000
BseMI	GCAATG	1 cut(s)	506
BseMII	CTCAG	9 cut(s)	55, 163, 410, 826, 941, 1421, 2025, 2148, 2223
BseNI	ACTGG	4 cut(s)	610, 1574, 1985, 2755
BseRI	GAGGAG	2 cut(s)	708, 833
BseSI	GKGCMC	1 cut(s)	1711
BseXI	GCAGC	8 cut(s)	559, 1012, 1571, 1904, 2438, 2638, 2917, 2920
BsgI	GTGCAG	3 cut(s)	1911, 2043, 2187
Bsh1236I	CGCG	2 cut(s)	457, 559
Bsh1285I	CGRYCG	3 cut(s)	878, 1690, 1744
BshFI	GGCC	5 cut(s)	1466, 1709, 2440, 2858, 2993
BshNI	GGYRCC	3 cut(s)	138, 1570, 2467
BshVI	ATCGAT	1 cut(s)	1365
BsiEI	CGRYCG	3 cut(s)	878, 1690, 1744
BsiHKAI	GWGCWC	1 cut(s)	2523
BsiSI	CCGG	6 cut(s)	51, 136, 229, 451, 1192, 2894
BslFI	GGGAC	5 cut(s)	272, 1653, 1997, 2068, 2877
BslI	CCNNNNNNNGG	6 cut(s)	457, 558, 1717, 2447, 2477, 3000
BsmAI	GTCTC	4 cut(s)	542, 1435, 2252, 2991
BsmBI	CGTCTC	1 cut(s)	1435
BsmFI	GGGAC	5 cut(s)	272, 1653, 1997, 2068, 2877
BsmI	GAATGC	5 cut(s)	529, 769, 971, 2127, 2870
BsnI	GGCC	5 cut(s)	1466, 1709, 2440, 2858, 2993
Bso31I	GGTCTC	2 cut(s)	542, 2252
Bsp120I	GGGCCC	1 cut(s)	1707
Bsp1286I	GDGCHC	3 cut(s)	42, 1711, 2523
Bsp1407I	TGTACA	1 cut(s)	2482
Bsp1720I	GCTNAGC	2 cut(s)	41, 824
Bsp19I	CCATGG	1 cut(s)	1331
BspACI	CCGC	7 cut(s)	5, 70, 76, 275, 457, 559, 625
BspANI	GGCC	5 cut(s)	1466, 1709, 2440, 2858, 2993
BspCNI	CTCAG	9 cut(s)	54, 162, 409, 825, 940, 1420, 2024, 2147, 2224
BspDI	ATCGAT	1 cut(s)	1365
BspFNI	CGCG	2 cut(s)	457, 559
BspHI	TCATGA	3 cut(s)	1450, 2593, 2925
BspLI	GGNNCC	7 cut(s)	55, 140, 1572, 1709, 1985, 2469, 2857
BspMAI	CTGCAG	1 cut(s)	1632
BspMI	ACCTGC	1 cut(s)	2071
BspPI	GGATC	1 cut(s)	2156
BspT107I	GGYRCC	3 cut(s)	138, 1570, 2467
BspTNI	GGTCTC	2 cut(s)	542, 2252
BsrBI	CCGCTC	1 cut(s)	78
BsrDI	GCAATG	1 cut(s)	506
BsrFI	RCCGGY	2 cut(s)	50, 1191
BsrGI	TGTACA	1 cut(s)	2482
BsrI	ACTGG	4 cut(s)	610, 1574, 1985, 2755
BssAI	RCCGGY	2 cut(s)	50, 1191
BssECI	CCNNGG	7 cut(s)	33, 34, 134, 576, 1331, 2050, 2859
BssNI	GRCGYC	1 cut(s)	279
BssT1I	CCWWGG	3 cut(s)	1331, 2050, 2859
Bst2UI	CCWGG	4 cut(s)	22, 35, 578, 1536
Bst6I	CTCTTC	3 cut(s)	1206, 1427, 2805
BstACI	GRCGYC	1 cut(s)	279
BstAPI	GCANNNNNTGC	2 cut(s)	1006, 1577
BstAUI	TGTACA	1 cut(s)	2482
BstC8I	GCNNGC	7 cut(s)	94, 131, 277, 1468, 1679, 1922, 2578
BstDSI	CCRYGG	1 cut(s)	1331
BstF5I	GGATG	5 cut(s)	850, 1327, 1606, 1813, 2670
BstFNI	CGCG	2 cut(s)	457, 559
BstHHI	GCGC	4 cut(s)	457, 469, 559, 1996
BstMAI	GTCTC	4 cut(s)	542, 1435, 2252, 2991
BstMCI	CGRYCG	3 cut(s)	878, 1690, 1744
BstNI	CCWGG	4 cut(s)	22, 35, 578, 1536
BstNSI	RCATGY	4 cut(s)	1547, 1681, 2034, 2501
BstSCI	CCNGG	6 cut(s)	20, 33, 134, 450, 576, 1534
BstSFI	CTRYAG	3 cut(s)	219, 1120, 1628
BstSLI	GKGCMC	1 cut(s)	1711
BstUI	CGCG	2 cut(s)	457, 559
BstV1I	GCAGC	8 cut(s)	559, 1012, 1571, 1904, 2438, 2638, 2917, 2920
BstV2I	GAAGAC	3 cut(s)	480, 699, 1778
BstX2I	RGATCY	2 cut(s)	1202, 1714
BstXI	CCANNNNNNTGG	2 cut(s)	1980, 2989
BstYI	RGATCY	2 cut(s)	1202, 1714
Bsu15I	ATCGAT	1 cut(s)	1365
Bsu36I	CCTNAGG	1 cut(s)	1703
BsuI	GTATCC	1 cut(s)	2941
BsuRI	GGCC	5 cut(s)	1466, 1709, 2440, 2858, 2993
BsuTUI	ATCGAT	1 cut(s)	1365
BtgI	CCRYGG	1 cut(s)	1331
BtrI	CACGTC	1 cut(s)	2867
BtsCI	GGATG	5 cut(s)	850, 1327, 1606, 1813, 2670
BtsI	GCAGTG	4 cut(s)	106, 321, 1625, 1939
BtsIMutI	CAGTG	7 cut(s)	106, 321, 1625, 1773, 1939, 2328, 2362
BveI	ACCTGC	1 cut(s)	2071
Cac8I	GCNNGC	7 cut(s)	94, 131, 277, 1468, 1679, 1922, 2578
CaiI	CAGNNNCTG	1 cut(s)	1006
CciI	TCATGA	3 cut(s)	1450, 2593, 2925
CfoI	GCGC	4 cut(s)	457, 469, 559, 1996
Cfr10I	RCCGGY	2 cut(s)	50, 1191
Cfr13I	GGNCC	5 cut(s)	1189, 1707, 1708, 1984, 2856
ClaI	ATCGAT	1 cut(s)	1365
CseI	GACGC	2 cut(s)	268, 1499
CsiI	ACCWGGT	1 cut(s)	20
Csp6I	GTAC	9 cut(s)	48, 1684, 2348, 2353, 2483, 2537, 2620, 2795, 2965
CviQI	GTAC	9 cut(s)	48, 1684, 2348, 2353, 2483, 2537, 2620, 2795, 2965
DraIII	CACNNNGTG	1 cut(s)	1648
DrdI	GACNNNNNNGTC	1 cut(s)	886
DriI	GACNNNNNGTC	1 cut(s)	1495
DseDI	GACNNNNNNGTC	1 cut(s)	886
EaeI	YGGCCR	1 cut(s)	2991
Eam1104I	CTCTTC	3 cut(s)	1206, 1427, 2805
Eam1105I	GACNNNNNGTC	1 cut(s)	1495
EarI	CTCTTC	3 cut(s)	1206, 1427, 2805
Ecl136II	GAGCTC	1 cut(s)	2521
Eco130I	CCWWGG	3 cut(s)	1331, 2050, 2859
Eco24I	GRGCYC	3 cut(s)	42, 1711, 2523
Eco31I	GGTCTC	2 cut(s)	542, 2252
Eco32I	GATATC	1 cut(s)	1518
Eco47I	GGWCC	2 cut(s)	1189, 1984
Eco53kI	GAGCTC	1 cut(s)	2521
Eco57I	CTGAAG	2 cut(s)	2052, 2786
Eco81I	CCTNAGG	1 cut(s)	1703
EcoICRI	GAGCTC	1 cut(s)	2521
EcoO109I	RGGNCCY	4 cut(s)	1707, 1708, 1984, 2856
EcoRI	GAATTC	1 cut(s)	1446
EcoRII	CCWGG	4 cut(s)	20, 33, 576, 1534
EcoRV	GATATC	1 cut(s)	1518
EcoT14I	CCWWGG	3 cut(s)	1331, 2050, 2859
EcoT22I	ATGCAT	2 cut(s)	769, 2317
EcoT38I	GRGCYC	3 cut(s)	42, 1711, 2523
ErhI	CCWWGG	3 cut(s)	1331, 2050, 2859
Esp3I	CGTCTC	1 cut(s)	1435
FalI	AAGNNNNNCTT	2 cut(s)	1086, 1118
FaqI	GGGAC	5 cut(s)	272, 1653, 1997, 2068, 2877
FauI	CCCGC	3 cut(s)	77, 268, 450
FbaI	TGATCA	3 cut(s)	307, 367, 2596
FblI	GTMKAC	3 cut(s)	878, 1499, 1744
Fnu4HI	GCNGC	8 cut(s)	573, 1001, 1560, 1893, 2452, 2652, 2906, 2909
FokI	GGATG	5 cut(s)	857, 1334, 1613, 1800, 2657
FriOI	GRGCYC	3 cut(s)	42, 1711, 2523
Fsp4HI	GCNGC	8 cut(s)	573, 1001, 1560, 1893, 2452, 2652, 2906, 2909
FspBI	CTAG	5 cut(s)	735, 1010, 1712, 2016, 2550
GlaI	GCGC	4 cut(s)	456, 468, 558, 1995
GluI	GCNGC	8 cut(s)	573, 1001, 1560, 1893, 2452, 2652, 2906, 2909
GsuI	CTGGAG	1 cut(s)	1852
HaeIII	GGCC	5 cut(s)	1466, 1709, 2440, 2858, 2993
HapII	CCGG	6 cut(s)	51, 136, 229, 451, 1192, 2894
HgaI	GACGC	2 cut(s)	268, 1499
HhaI	GCGC	4 cut(s)	457, 469, 559, 1996
Hin1I	GRCGYC	1 cut(s)	279
Hin6I	GCGC	4 cut(s)	455, 467, 557, 1994
HinP1I	GCGC	4 cut(s)	455, 467, 557, 1994
HincII	GTYRAC	3 cut(s)	879, 1020, 1745
HindII	GTYRAC	3 cut(s)	879, 1020, 1745
HindIII	AAGCTT	1 cut(s)	2394
HpaII	CCGG	6 cut(s)	51, 136, 229, 451, 1192, 2894
HphI	GGTGA	4 cut(s)	22, 506, 659, 2193
Hpy166II	GTNNAC	7 cut(s)	879, 1020, 1500, 1745, 2098, 2483, 2696
Hpy8I	GTNNAC	7 cut(s)	879, 1020, 1500, 1745, 2098, 2483, 2696
Hpy99I	CGWCG	3 cut(s)	217, 248, 2627
HpyAV	CCTTC	8 cut(s)	219, 390, 493, 979, 1293, 1630, 1775, 2914
HpyCH4IV	ACGT	1 cut(s)	2866
HpySE526I	ACGT	1 cut(s)	2866
Hsp92I	GRCGYC	1 cut(s)	279
HspAI	GCGC	4 cut(s)	455, 467, 557, 1994
Ksp22I	TGATCA	3 cut(s)	307, 367, 2596
LmnI	GCTCC	6 cut(s)	59, 322, 721, 820, 1975, 2518
Lsp1109I	GCAGC	8 cut(s)	559, 1012, 1571, 1904, 2438, 2638, 2917, 2920
MabI	ACCWGGT	1 cut(s)	20
MaeI	CTAG	5 cut(s)	735, 1010, 1712, 2016, 2550
MaeII	ACGT	1 cut(s)	2866
MaeIII	GTNAC	3 cut(s)	28, 647, 2188
MbiI	CCGCTC	1 cut(s)	78
MfeI	CAATTG	2 cut(s)	157, 1395
MflI	RGATCY	2 cut(s)	1202, 1714
MhlI	GDGCHC	3 cut(s)	42, 1711, 2523
MlsI	TGGCCA	1 cut(s)	2993
MluNI	TGGCCA	1 cut(s)	2993
MlyI	GAGTC	8 cut(s)	242, 896, 1136, 1354, 1448, 1736, 2621, 2824
MmeI	TCCRAC	2 cut(s)	81, 885
Mox20I	TGGCCA	1 cut(s)	2993
Mph1103I	ATGCAT	2 cut(s)	769, 2317
MroXI	GAANNNNTTC	3 cut(s)	337, 1656, 1785
MscI	TGGCCA	1 cut(s)	2993
MseI	TTAA	7 cut(s)	356, 462, 705, 957, 2160, 2399, 3019
MslI	CAYNNNNRTG	3 cut(s)	1224, 1944, 2598
Msp20I	TGGCCA	1 cut(s)	2993
MspA1I	CMGCKG	1 cut(s)	700
MspI	CCGG	6 cut(s)	51, 136, 229, 451, 1192, 2894
MspR9I	CCNGG	6 cut(s)	22, 35, 136, 452, 578, 1536
MunI	CAATTG	2 cut(s)	157, 1395
Mva1269I	GAATGC	5 cut(s)	529, 769, 971, 2127, 2870
MvaI	CCWGG	4 cut(s)	22, 35, 578, 1536
MvnI	CGCG	2 cut(s)	457, 559
NciI	CCSGG	2 cut(s)	136, 452
NcoI	CCATGG	1 cut(s)	1331
NlaIV	GGNNCC	7 cut(s)	55, 140, 1572, 1709, 1985, 2469, 2857
NmuCI	GTSAC	3 cut(s)	28, 647, 2188
NsiI	ATGCAT	2 cut(s)	769, 2317
NspI	RCATGY	4 cut(s)	1547, 1681, 2034, 2501
PaeI	GCATGC	1 cut(s)	1681
PagI	TCATGA	3 cut(s)	1450, 2593, 2925
PaqCI	CACCTGC	1 cut(s)	2071
PasI	CCCWGGG	1 cut(s)	34
PcsI	WCGNNNNNNNCGW	1 cut(s)	249
PctI	GAATGC	5 cut(s)	529, 769, 971, 2127, 2870
PdmI	GAANNNNTTC	3 cut(s)	337, 1656, 1785
PflFI	GACNNNGTC	1 cut(s)	2244
PfoI	TCCNGGA	1 cut(s)	1534
PkrI	GCNGC	8 cut(s)	574, 1002, 1561, 1894, 2453, 2653, 2907, 2910
Ple19I	CGATCG	1 cut(s)	1690
PleI	GAGTC	8 cut(s)	242, 895, 1135, 1353, 1448, 1735, 2621, 2824
PpsI	GAGTC	8 cut(s)	242, 895, 1135, 1353, 1448, 1735, 2621, 2824
PpuMI	RGGWCCY	1 cut(s)	1984
Psp124BI	GAGCTC	1 cut(s)	2523
Psp5II	RGGWCCY	1 cut(s)	1984
Psp6I	CCWGG	4 cut(s)	20, 33, 576, 1534
PspGI	CCWGG	4 cut(s)	20, 33, 576, 1534
PspN4I	GGNNCC	7 cut(s)	55, 140, 1572, 1709, 1985, 2469, 2857
PspOMI	GGGCCC	1 cut(s)	1707
PspPI	GGNCC	5 cut(s)	1189, 1707, 1708, 1984, 2856
PspPPI	RGGWCCY	1 cut(s)	1984
PstI	CTGCAG	1 cut(s)	1632
PstNI	CAGNNNCTG	1 cut(s)	1006
PsuI	RGATCY	2 cut(s)	1202, 1714
PsyI	GACNNNGTC	1 cut(s)	2244
PvuI	CGATCG	1 cut(s)	1690
PvuII	CAGCTG	1 cut(s)	700
RsaI	GTAC	9 cut(s)	49, 1685, 2349, 2354, 2484, 2538, 2621, 2796, 2966
RsaNI	GTAC	9 cut(s)	48, 1684, 2348, 2353, 2483, 2537, 2620, 2795, 2965
RseI	CAYNNNNRTG	3 cut(s)	1224, 1944, 2598
SacI	GAGCTC	1 cut(s)	2523
SalI	GTCGAC	2 cut(s)	877, 1743
SaqAI	TTAA	7 cut(s)	356, 462, 705, 957, 2160, 2399, 3019
SatI	GCNGC	8 cut(s)	573, 1001, 1560, 1893, 2452, 2652, 2906, 2909
Sau96I	GGNCC	5 cut(s)	1189, 1707, 1708, 1984, 2856
SchI	GAGTC	8 cut(s)	242, 896, 1136, 1354, 1448, 1736, 2621, 2824
ScrFI	CCNGG	6 cut(s)	22, 35, 136, 452, 578, 1536
SduI	GDGCHC	3 cut(s)	42, 1711, 2523
SexAI	ACCWGGT	1 cut(s)	20
SfcI	CTRYAG	3 cut(s)	219, 1120, 1628
SinI	GGWCC	2 cut(s)	1189, 1984
SmiMI	CAYNNNNRTG	3 cut(s)	1224, 1944, 2598
SmlI	CTYRAG	1 cut(s)	1898
SmoI	CTYRAG	1 cut(s)	1898
SphI	GCATGC	1 cut(s)	1681
SsiI	CCGC	7 cut(s)	5, 70, 76, 275, 457, 559, 625
SspMI	CTAG	5 cut(s)	735, 1010, 1712, 2016, 2550
SstI	GAGCTC	1 cut(s)	2523
StyD4I	CCNGG	6 cut(s)	20, 33, 134, 450, 576, 1534
StyI	CCWWGG	3 cut(s)	1331, 2050, 2859
TaiI	ACGT	1 cut(s)	2869
TaqI	TCGA	7 cut(s)	212, 341, 878, 911, 1365, 1744, 2509
TaqII	GACCGA	1 cut(s)	566
TatI	WGTACW	4 cut(s)	2347, 2352, 2482, 2536
Tru1I	TTAA	7 cut(s)	356, 462, 705, 957, 2160, 2399, 3019
Tru9I	TTAA	7 cut(s)	356, 462, 705, 957, 2160, 2399, 3019
TscAI	CASTG	7 cut(s)	113, 328, 1632, 1780, 1946, 2335, 2362
TseFI	GTSAC	3 cut(s)	28, 647, 2188
TseI	GCWGC	8 cut(s)	572, 1000, 1559, 1892, 2451, 2651, 2905, 2908
Tsp45I	GTSAC	3 cut(s)	28, 647, 2188
TspGWI	ACGGA	2 cut(s)	351, 857
TspRI	CASTG	7 cut(s)	113, 328, 1632, 1780, 1946, 2335, 2362
Tth111I	GACNNNGTC	1 cut(s)	2244
VpaK11BI	GGWCC	2 cut(s)	1189, 1984
XapI	RAATTY	6 cut(s)	994, 1256, 1337, 1446, 2263, 2561
XceI	RCATGY	4 cut(s)	1547, 1681, 2034, 2501
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1338
XmiI	GTMKAC	3 cut(s)	878, 1499, 1744
XmnI	GAANNNNTTC	3 cut(s)	337, 1656, 1785
XspI	CTAG	5 cut(s)	735, 1010, 1712, 2016, 2550
Zsp2I	ATGCAT	2 cut(s)	769, 2317

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.