Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005866

Physical Location & Seq

Genomic Coordinates Forward (+)

8979955 .. 8986858

Gene Structure 6904 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ29378

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3033 bp

ATGGCGGTGAGGCTAACCACCTGGTTTGTGAGCCAGGGGATTAGCATTTCCGGTTCAAACTCCAGGCGCTCACATTCAAATCCGCGCTTCTCATTTGCTTCCTCCTCTCCTGCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTGTAAGCTACGCCACCGGAATCTCAGTTGTACTTGCCTGCAGAAGAGGCAAAGAGCTAGGAAGAAATCCTCTCAGGAACAGTCTTCTGCGGATTTCCTGCCAAGTGGCGAAGAGGACTCTGAATCTGAGACTGCTTCGGTCGGCATGATTCCTCTTCTGAACTCAGAAAGCGTATCTGATGATGATGTTAATACTATCAATTCAAACGATTCGATTTCGAATGCTCTTACTACATCTGATCAAGCAGTACCTTCGGATTTGGTTGGAATGATAAGGAATGCGGAGAAAAATATTCTTCTCTTGAATCAAGCTCGGGTTAGTGCTCTTCAAGACCTTGACAAGATTCTATCTGAAAAGGAAGAATTGCAAGGAGAAATGAATGCTTTGGAAATGAGATTGGCAGAGACTGATGCACGTATCAGAGTGGCTGCTCAAGAGAAAGTAAGGATGGAACTCCTCGGAGACCACTTAAACCAGATGCGGAATGAACAGAATTTCAATGGTGATGGTGCGGAAACAAGCAATGGGGTTGAGATTTTTGAGAGTGAAAGTGAGCTATTTAATGAAGAAGCTCCTTTGCCATACAAAAGTAGCATCAATTCGCTTGTAGCAAATCTTAGTTCATTGAGATTAGAAAATGTGTCACTCAGGAATGATATACAGGAACTCAGAGAGCAGCTTAGTAATATGAAAAATACGGATGAACGTGTGGTCATGCTTGAAAAACAACGTTCGACTTTGGACTCTGCCCTAAAAGATTTGGAGTCCAAATTGGTAGTTTCTCAGGAAGATGTTTCCAAACTTTCCAATCTGAAGGTTGAATGCAAAGGTTTATGGGAAAAGGTGGAGAATTTGCAAGTACTGCTAGATAAGTCGACCAAGCAGGCAGATCAAGCTATTATAGTGTTACAGCAAAATCAGGAGATTCGAAAAAAGGTTGATAAATTAGAAGAATCTCTTGAAAAAGCTAATGTGTATAAGGAGTCATCAGAAAAGATGCAACAGTATAATGAGCTTATGCAACAAAAGATAAAACTAATGGAGGACCGGCTTCAGCGTTCGGATGAAGAGATACATTCTTATGTTCAGTTATATCAAGAATCTGTGGAGGAATTTCAAGATACCTTAAATACTCTAAAAGAAGAAAGCAAGAGAAGGGTGATGGATGAACCTGTTGATGACATGCCTTGGGAATTTTGGAGCCGTTTATTGCTTATAATAGATGGTTGGTTGTTGGAAAAGAAAATATCAATTGATGATGCAAAGGCGTTGAGAGAAATGGTATGGAAGAGAGATCGACGGATTCATGACTCATACATGGCATGCAAGGAAAAGAATGTAAATGAGGCTGTGACTACATTTCTCAGACTGATATCATCACAAACAAGTTCAGGATTGCATGTCGTTCATATCGCAGCAGAGATGGCACCAGTTGCTAAGGTCGGTGGTTTGGGTGATGTTGTAGCTGGTCTTAGTAAAGCATTGCAGAAGAAGGGACACCTTGTGGAAATTATTCTTCCCAAATATGATTGCATGGACTACGATCGTGTTCGTGACCTAAGGGCCCTAGATGTGGCAGTAGAGTCGTACTTCGATGGTCGACTATTCAAGAATAAAATCTGGGTTGGCACTGTTGAAGGTCTTCCTGTATATTTTATTGAGCCTCTTCATCCTGATAAGCTCTTCTGGAGAGGACAGTTTTATGGAGAACGTGATGATTTCAAACGTTTTTCCTTCTTCAGTCGTGCAGCCCTTGAGTTACTTCTTCAAGCTGGCAAGAAACCTGACATTATTCATTGTCATGATTGGCAGACAGCTTTCGTGGCTCCACTTTATTGGGACTTATATGCCCCAAAGGGTTTAAACTCGGCTAGGATATGTTTTACCTGCCACAACTTTGAATACCAAGGGACTGCACCTGCTTCAGACTTGGCTTCTTGTGGTCTTGATGTCGGCCAGCTAAACAGACCAGATAGAATGCAGGACAACTCAGCACATGATAGGATCAATCCGGTTAAGGGTGCAGTTGTATTCTCTAATATCGTGACAACGGTATCACTTACCTATGCGCAAGAGGTGCGGAGCGCTGAGGGTGGACGTGGTCTCCATTCAACACTTAATTTTCATTCTAAAAAGTTCGTTGGAATTCTCAATGGAATTGATGCTGATGCATGGAATCCTGCCACTGATGCATATCTCAAAGTACAGTACACTGCCAATGATCTTGGGGGGAAAGCAGAAAATAAGGAAGCTATTAGAAAGAGTCTAGGGCTTTCATATGCCGATGTCAGGAGACCATTGGTGGGATGCATAACGAGATTGGTGCCACAGAAAGGTGTACATCTCATCAGACATGCGATTTATAGAACATTGGAGCTAGGAGGGCAGTTTGTTCTTCTGGGTTCAAGTCCAGTACATCATATTCAGAAGGAATTTGAGGCAATTGCAAACCATTTTCAGAGTCATGATCATATTAGGTTGATATTGAAGTATGATGAAACTCTTTCCCATTCCATTTATGCTGCTTCTGATATGTTCATCATTCCATCAATCTTTGAGCCTTGTGGACTTACACAGATGATAGCAATGAGATATGGTTCTATACCCATTGCAAGAAAAACTGGTGGACTAAATGATAGTGTCTTTGATGTTGATGATGACACCGTACCTCTTCAGTTTCGAAATGGATATTCATTTTTGTCTCCTGATGAACAGGGGCTAAACGGTGCTTTGGAACGTGCATTTAACCACTACCTAAGTAAGCCTGAGAGTTGGCGGCAGCTTGTTCAGAAGGATATGAACATAGATTTCAGTTGGGATACTTCTGCTTCACAGTACGAAGAACTCTACTCTAAGTCCGTGGCCAGAGCAAGGGCCACAATTCGCCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009011 GO:0009058 GO:0009059 GO:0009250 GO:0009507 GO:0009536 GO:0009987 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0019252 GO:0033692 GO:0034637 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026

Pfam Domains

Protein Families

Glyco_transf_5 Glycos_transf_1

Protein Analysis

1010

Amino Acids

114.08

Weight (kDa)

5.71

Isoelectric Point (pI)

48.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_5	PF08323	514 - 754	4.9e-65	Starch synthase catalytic domain
Glyco_transf_4	PF13439	528 - 682	4.8e-08	Glycosyltransferase Family 4
Glycos_transf_1	PF00534	811 - 972	1.6e-09	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	813 - 931	9.7e-09	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005866)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18240
fragaria_vesca	FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130 FvH4_3g06130
malus_domestica	MD05G1289600.v1.1 MD10G1265500.v1.1
prunus_persica	Prupe.4G076100_v2.0.a1
pyrus_communis	pycom05g26670 pycom10g22140
rosa_chinensis	RchiOBHm_Chr5g0013241
rosa_laevigata	RLG00000032000
rosa_multiflora	Rmu_co8500739.1_g000001 Rmu_sc0004521.1_g000017
rosa_roxburghii	Rroxscaffold_1G00063270
rosa_rugosa	Rorug05G0008200.1 Rorug05G0008300.1 Rorug05G0008400
rosa_samantha	Rh5AG103000 Rh5BG099800 Rh5CG111200 Rh5DG098600
rosa_wichuraiana	Rw5G008990

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1358
AarI	CACCTGC	1 cut(s)	2068
Acc16I	TGCGCA	1 cut(s)	2211
Acc36I	ACCTGC	2 cut(s)	2036, 2068
AccB1I	GGYRCC	2 cut(s)	1567, 2464
AccI	GTMKAC	2 cut(s)	1016, 1741
AccII	CGCG	1 cut(s)	85
AciI	CCGC	8 cut(s)	5, 83, 230, 422, 622, 653, 2221, 2917
AclI	AACGTT	2 cut(s)	871, 1867
AclWI	GGATC	1 cut(s)	2153
AcoI	YGGCCR	2 cut(s)	2095, 3003
AcsI	RAATTY	6 cut(s)	634, 991, 1253, 1334, 2286, 2573
AcuI	CTGAAG	5 cut(s)	974, 1178, 1864, 2049, 2798
AdeI	CACNNNGTG	1 cut(s)	1645
AfeI	AGCGCT	1 cut(s)	2227
AfiI	CCNNNNNNNGG	6 cut(s)	1714, 2159, 2430, 2444, 2474, 3012
AflIII	ACRYGT	1 cut(s)	847
AjiI	CACGTC	1 cut(s)	2240
AjnI	CCWGG	3 cut(s)	20, 33, 62
Alw21I	GWGCWC	1 cut(s)	466
Alw26I	GTCTC	6 cut(s)	263, 539, 597, 2249, 2428, 2847
AlwI	GGATC	1 cut(s)	2153
AlwNI	CAGNNNCTG	1 cut(s)	548
Ama87I	CYCGRG	1 cut(s)	453
Aor51HI	AGCGCT	1 cut(s)	2227
AoxI	GGCC	4 cut(s)	1704, 2095, 3003, 3015
ApaI	GGGCCC	1 cut(s)	1708
ApeKI	GCWGC	6 cut(s)	569, 817, 1556, 1889, 2663, 2920
ApoI	RAATTY	6 cut(s)	634, 991, 1253, 1334, 2286, 2573
Asp700I	GAANNNNTTC	2 cut(s)	1653, 1782
AspLEI	GCGC	4 cut(s)	69, 87, 2212, 2228
AspS9I	GGNCC	4 cut(s)	1186, 1704, 1705, 3015
AsuHPI	GGTGA	4 cut(s)	19, 656, 1312, 1607
AsuII	TTCGAA	3 cut(s)	359, 1069, 2821
AvaI	CYCGRG	1 cut(s)	453
AvaII	GGWCC	1 cut(s)	1186
AxyI	CCTNAGG	1 cut(s)	1700
BaeGI	GKGCMC	1 cut(s)	1708
BaeI	ACNNNNGTAYC	2 cut(s)	2178, 2211
BalI	TGGCCA	1 cut(s)	3005
BanI	GGYRCC	2 cut(s)	1567, 2464
BanII	GRGCYC	1 cut(s)	1708
BbsI	GAAGAC	2 cut(s)	216, 1775
Bbv12I	GWGCWC	1 cut(s)	466
BbvCI	CCTCAGC	1 cut(s)	2229
BbvI	GCAGC	6 cut(s)	556, 829, 1568, 1901, 2650, 2932
BccI	CCATC	7 cut(s)	583, 641, 1297, 1358, 1558, 1730, 2695
BceAI	ACGGC	1 cut(s)	1329
BcgI	CGANNNNNNTGC	6 cut(s)	375, 409, 1707, 1741, 2446, 2480
BciT130I	CCWGG	3 cut(s)	22, 35, 64
BciVI	GTATCC	1 cut(s)	2953
BclI	TGATCA	2 cut(s)	379, 2608
BcoDI	GTCTC	6 cut(s)	263, 539, 597, 2249, 2428, 2847
BfaI	CTAG	6 cut(s)	198, 1007, 1709, 2013, 2408, 2519
BfmI	CTRYAG	1 cut(s)	179
BfoI	RGCGCY	2 cut(s)	70, 2229
BfuAI	ACCTGC	2 cut(s)	2036, 2068
BfuI	GTATCC	1 cut(s)	2953
BisI	GCNGC	7 cut(s)	570, 818, 1557, 1890, 2664, 2918, 2921
BlsI	GCNGC	7 cut(s)	571, 819, 1558, 1891, 2665, 2919, 2922
BmcAI	AGTACT	1 cut(s)	1002
Bme1390I	CCNGG	3 cut(s)	22, 35, 64
Bme18I	GGWCC	1 cut(s)	1186
BmeT110I	CYCGRG	1 cut(s)	453
BmgBI	CACGTC	1 cut(s)	2240
BmgT120I	GGNCC	4 cut(s)	1186, 1704, 1705, 3015
BmiI	GGNNCC	5 cut(s)	1343, 1569, 1706, 1968, 2466
BmrFI	CCNGG	3 cut(s)	22, 35, 64
BmsI	GCATC	9 cut(s)	541, 609, 744, 1128, 1390, 2293, 2299, 2320, 2438
BpiI	GAAGAC	2 cut(s)	216, 1775
BpmI	CTGGAG	2 cut(s)	46, 1849
Bpu10I	CCTNAGC	2 cut(s)	1578, 2229
Bpu14I	TTCGAA	3 cut(s)	359, 1069, 2821
BpuEI	CTTGAG	2 cut(s)	558, 1916
BsaAI	YACGTR	1 cut(s)	557
BsaBI	GATNNNNATC	1 cut(s)	2334
BsaI	GGTCTC	3 cut(s)	597, 2249, 2428
BsaJI	CCNNGG	5 cut(s)	34, 598, 1328, 2047, 3000
BsaWI	WCCGGW	3 cut(s)	50, 156, 2152
BsaXI	ACNNNNNCTCC	2 cut(s)	2229, 2259
Bsc4I	CCNNNNNNNGG	6 cut(s)	1714, 2159, 2430, 2444, 2474, 3012
Bse118I	RCCGGY	1 cut(s)	1188
Bse1I	ACTGG	3 cut(s)	1571, 2552, 2767
Bse21I	CCTNAGG	1 cut(s)	1700
Bse3DI	GCAATG	4 cut(s)	670, 1622, 2733, 2748
Bse8I	GATNNNNATC	1 cut(s)	2334
BseBI	CCWGG	3 cut(s)	22, 35, 64
BseDI	CCNNGG	5 cut(s)	34, 598, 1328, 2047, 3000
BseGI	GGATG	6 cut(s)	594, 847, 1210, 1312, 1810, 2453
BseJI	GATNNNNATC	1 cut(s)	2334
BseLI	CCNNNNNNNGG	6 cut(s)	1714, 2159, 2430, 2444, 2474, 3012
BseMI	GCAATG	4 cut(s)	670, 1622, 2733, 2748
BseNI	ACTGG	3 cut(s)	1571, 2552, 2767
BseRI	GAGGAG	2 cut(s)	94, 587
BseSI	GKGCMC	1 cut(s)	1708
BseXI	GCAGC	6 cut(s)	556, 829, 1568, 1901, 2650, 2932
BsgI	GTGCAG	3 cut(s)	1908, 2040, 2184
Bsh1236I	CGCG	1 cut(s)	85
Bsh1285I	CGRYCG	2 cut(s)	282, 1687
BshFI	GGCC	4 cut(s)	1706, 2097, 3005, 3017
BshNI	GGYRCC	2 cut(s)	1567, 2464
BsiEI	CGRYCG	2 cut(s)	282, 1687
BsiHKAI	GWGCWC	1 cut(s)	466
BsiHKCI	CYCGRG	1 cut(s)	453
BsiSI	CCGG	4 cut(s)	51, 157, 1189, 2153
BslFI	GGGAC	3 cut(s)	1650, 1994, 2065
BslI	CCNNNNNNNGG	6 cut(s)	1714, 2159, 2430, 2444, 2474, 3012
BsmAI	GTCTC	6 cut(s)	263, 539, 597, 2249, 2428, 2847
BsmFI	GGGAC	3 cut(s)	1650, 1994, 2065
BsmI	GAATGC	5 cut(s)	367, 424, 526, 968, 2124
BsnI	GGCC	4 cut(s)	1706, 2097, 3005, 3017
Bso31I	GGTCTC	3 cut(s)	597, 2249, 2428
BsoBI	CYCGRG	1 cut(s)	453
Bsp119I	TTCGAA	3 cut(s)	359, 1069, 2821
Bsp120I	GGGCCC	1 cut(s)	1704
Bsp1286I	GDGCHC	2 cut(s)	466, 1708
Bsp1407I	TGTACA	1 cut(s)	2479
Bsp143I	GATC	7 cut(s)	379, 1030, 1435, 1684, 2145, 2362, 2608
BspACI	CCGC	8 cut(s)	5, 83, 230, 422, 622, 653, 2221, 2917
BspANI	GGCC	4 cut(s)	1706, 2097, 3005, 3017
BspFNI	CGCG	1 cut(s)	85
BspHI	TCATGA	3 cut(s)	1447, 1942, 2605
BspLI	GGNNCC	5 cut(s)	1343, 1569, 1706, 1968, 2466
BspMAI	CTGCAG	1 cut(s)	183
BspMI	ACCTGC	2 cut(s)	2036, 2068
BspPI	GGATC	1 cut(s)	2153
BspQI	GCTCTTC	2 cut(s)	471, 1829
BspT104I	TTCGAA	3 cut(s)	359, 1069, 2821
BspT107I	GGYRCC	2 cut(s)	1567, 2464
BspTNI	GGTCTC	3 cut(s)	597, 2249, 2428
BsrDI	GCAATG	4 cut(s)	670, 1622, 2733, 2748
BsrFI	RCCGGY	1 cut(s)	1188
BsrGI	TGTACA	1 cut(s)	2479
BsrI	ACTGG	3 cut(s)	1571, 2552, 2767
BssAI	RCCGGY	1 cut(s)	1188
BssECI	CCNNGG	5 cut(s)	34, 598, 1328, 2047, 3000
BssMI	GATC	7 cut(s)	379, 1030, 1435, 1684, 2145, 2362, 2608
BssT1I	CCWWGG	2 cut(s)	1328, 2047
Bst2UI	CCWGG	3 cut(s)	22, 35, 64
Bst4CI	ACNGT	9 cut(s)	222, 1146, 1774, 1839, 2194, 2349, 2806, 2867, 2976
Bst6I	CTCTTC	9 cut(s)	179, 246, 300, 471, 1203, 1424, 1812, 1829, 2817
BstAPI	GCANNNNNTGC	3 cut(s)	1003, 1574, 2218
BstAUI	TGTACA	1 cut(s)	2479
BstBAI	YACGTR	1 cut(s)	557
BstBI	TTCGAA	3 cut(s)	359, 1069, 2821
BstC8I	GCNNGC	5 cut(s)	179, 1026, 1465, 1915, 2099
BstDSI	CCRYGG	1 cut(s)	3000
BstF5I	GGATG	6 cut(s)	594, 847, 1210, 1312, 1810, 2453
BstFNI	CGCG	1 cut(s)	85
BstH2I	RGCGCY	2 cut(s)	70, 2229
BstHHI	GCGC	4 cut(s)	69, 87, 2212, 2228
BstKTI	GATC	7 cut(s)	382, 1033, 1438, 1687, 2148, 2365, 2611
BstMAI	GTCTC	6 cut(s)	263, 539, 597, 2249, 2428, 2847
BstMBI	GATC	7 cut(s)	379, 1030, 1435, 1684, 2145, 2362, 2608
BstMCI	CGRYCG	2 cut(s)	282, 1687
BstMWI	GCNNNNNNNGC	9 cut(s)	187, 1003, 1034, 1565, 1574, 1964, 2218, 2330, 2524
BstNI	CCWGG	3 cut(s)	22, 35, 64
BstNSI	RCATGY	4 cut(s)	1327, 1467, 1544, 2498
BstSCI	CCNGG	3 cut(s)	20, 33, 62
BstSFI	CTRYAG	1 cut(s)	179
BstSLI	GKGCMC	1 cut(s)	1708
BstUI	CGCG	1 cut(s)	85
BstV1I	GCAGC	6 cut(s)	556, 829, 1568, 1901, 2650, 2932
BstV2I	GAAGAC	2 cut(s)	216, 1775
BstXI	CCANNNNNNTGG	1 cut(s)	1977
Bsu36I	CCTNAGG	1 cut(s)	1700
BsuI	GTATCC	1 cut(s)	2953
BsuRI	GGCC	4 cut(s)	1706, 2097, 3005, 3017
BtgI	CCRYGG	1 cut(s)	3000
BtrI	CACGTC	1 cut(s)	2240
BtsCI	GGATG	6 cut(s)	594, 847, 1210, 1312, 1810, 2453
BtsI	GCAGTG	1 cut(s)	2352
BtsIMutI	CAGTG	3 cut(s)	1770, 2325, 2352
BveI	ACCTGC	2 cut(s)	2036, 2068
Cac8I	GCNNGC	5 cut(s)	179, 1026, 1465, 1915, 2099
CaiI	CAGNNNCTG	1 cut(s)	548
CciI	TCATGA	3 cut(s)	1447, 1942, 2605
CfoI	GCGC	4 cut(s)	69, 87, 2212, 2228
Cfr10I	RCCGGY	1 cut(s)	1188
Cfr13I	GGNCC	4 cut(s)	1186, 1704, 1705, 3015
CsiI	ACCWGGT	1 cut(s)	20
CspCI	CAANNNNNGTGG	2 cut(s)	7, 42
DpnI	GATC	7 cut(s)	381, 1032, 1437, 1686, 2147, 2364, 2610
DpnII	GATC	7 cut(s)	379, 1030, 1435, 1684, 2145, 2362, 2608
DraI	TTTAAA	1 cut(s)	2004
DraIII	CACNNNGTG	1 cut(s)	1645
EaeI	YGGCCR	2 cut(s)	2095, 3003
Eam1104I	CTCTTC	9 cut(s)	179, 246, 300, 471, 1203, 1424, 1812, 1829, 2817
EarI	CTCTTC	9 cut(s)	179, 246, 300, 471, 1203, 1424, 1812, 1829, 2817
Eco130I	CCWWGG	2 cut(s)	1328, 2047
Eco24I	GRGCYC	1 cut(s)	1708
Eco31I	GGTCTC	3 cut(s)	597, 2249, 2428
Eco32I	GATATC	1 cut(s)	1515
Eco47I	GGWCC	1 cut(s)	1186
Eco47III	AGCGCT	1 cut(s)	2227
Eco57I	CTGAAG	5 cut(s)	974, 1178, 1864, 2049, 2798
Eco81I	CCTNAGG	1 cut(s)	1700
Eco88I	CYCGRG	1 cut(s)	453
EcoO109I	RGGNCCY	2 cut(s)	1704, 1705
EcoRI	GAATTC	1 cut(s)	2286
EcoRII	CCWGG	3 cut(s)	20, 33, 62
EcoRV	GATATC	1 cut(s)	1515
EcoT14I	CCWWGG	2 cut(s)	1328, 2047
EcoT22I	ATGCAT	3 cut(s)	2314, 2335, 2453
EcoT38I	GRGCYC	1 cut(s)	1708
ErhI	CCWWGG	2 cut(s)	1328, 2047
FalI	AAGNNNNNCTT	2 cut(s)	1083, 1115
FaqI	GGGAC	3 cut(s)	1650, 1994, 2065
FauNDI	CATATG	1 cut(s)	2419
FbaI	TGATCA	2 cut(s)	379, 2608
FblI	GTMKAC	2 cut(s)	1016, 1741
Fnu4HI	GCNGC	7 cut(s)	570, 818, 1557, 1890, 2664, 2918, 2921
FokI	GGATG	6 cut(s)	601, 854, 1217, 1319, 1797, 2460
FriOI	GRGCYC	1 cut(s)	1708
Fsp4HI	GCNGC	7 cut(s)	570, 818, 1557, 1890, 2664, 2918, 2921
FspBI	CTAG	6 cut(s)	198, 1007, 1709, 2013, 2408, 2519
FspI	TGCGCA	1 cut(s)	2211
GlaI	GCGC	4 cut(s)	68, 86, 2211, 2227
GluI	GCNGC	7 cut(s)	570, 818, 1557, 1890, 2664, 2918, 2921
GsuI	CTGGAG	2 cut(s)	46, 1849
HaeII	RGCGCY	2 cut(s)	70, 2229
HaeIII	GGCC	4 cut(s)	1706, 2097, 3005, 3017
HapII	CCGG	4 cut(s)	51, 157, 1189, 2153
HhaI	GCGC	4 cut(s)	69, 87, 2212, 2228
Hin6I	GCGC	4 cut(s)	67, 85, 2210, 2226
HinP1I	GCGC	4 cut(s)	67, 85, 2210, 2226
HincII	GTYRAC	2 cut(s)	1017, 1742
HindII	GTYRAC	2 cut(s)	1017, 1742
HpaII	CCGG	4 cut(s)	51, 157, 1189, 2153
HphI	GGTGA	4 cut(s)	19, 656, 1312, 1607
Hpy166II	GTNNAC	7 cut(s)	1017, 1742, 2237, 2352, 2480, 2708, 2768
Hpy8I	GTNNAC	7 cut(s)	1017, 1742, 2237, 2352, 2480, 2708, 2768
Hpy99I	CGWCG	1 cut(s)	1443
HpyAV	CCTTC	8 cut(s)	402, 949, 1290, 1627, 1772, 1885, 2563, 2926
HpyCH4III	ACNGT	9 cut(s)	222, 1146, 1774, 1839, 2194, 2349, 2806, 2867, 2976
HpyCH4IV	ACGT	7 cut(s)	556, 847, 871, 1852, 1867, 2239, 2878
HpyF10VI	GCNNNNNNNGC	9 cut(s)	187, 1003, 1034, 1565, 1574, 1964, 2218, 2330, 2524
HpySE526I	ACGT	7 cut(s)	556, 847, 871, 1852, 1867, 2239, 2878
HspAI	GCGC	4 cut(s)	67, 85, 2210, 2226
Ksp22I	TGATCA	2 cut(s)	379, 2608
Kzo9I	GATC	7 cut(s)	379, 1030, 1435, 1684, 2145, 2362, 2608
LguI	GCTCTTC	2 cut(s)	471, 1829
LmnI	GCTCC	5 cut(s)	718, 1341, 1972, 2223, 2515
Lsp1109I	GCAGC	6 cut(s)	556, 829, 1568, 1901, 2650, 2932
LweI	GCATC	9 cut(s)	541, 609, 744, 1128, 1390, 2293, 2299, 2320, 2438
MabI	ACCWGGT	1 cut(s)	20
MaeI	CTAG	6 cut(s)	198, 1007, 1709, 2013, 2408, 2519
MaeII	ACGT	7 cut(s)	556, 847, 871, 1852, 1867, 2239, 2878
MaeIII	GTNAC	6 cut(s)	783, 1047, 1492, 1694, 1899, 2185
MalI	GATC	7 cut(s)	381, 1032, 1437, 1686, 2147, 2364, 2610
MboI	GATC	7 cut(s)	379, 1030, 1435, 1684, 2145, 2362, 2608
MfeI	CAATTG	2 cut(s)	1392, 2583
MhlI	GDGCHC	2 cut(s)	466, 1708
MlsI	TGGCCA	1 cut(s)	3005
MluNI	TGGCCA	1 cut(s)	3005
MlyI	GAGTC	8 cut(s)	251, 878, 914, 1133, 1445, 1733, 2413, 2611
MmeI	TCCRAC	3 cut(s)	385, 1356, 2263
Mox20I	TGGCCA	1 cut(s)	3005
Mph1103I	ATGCAT	3 cut(s)	2314, 2335, 2453
MroXI	GAANNNNTTC	2 cut(s)	1653, 1782
MscI	TGGCCA	1 cut(s)	3005
MseI	TTAA	9 cut(s)	330, 611, 702, 1268, 2003, 2157, 2259, 2886, 3031
MslI	CAYNNNNRTG	2 cut(s)	1221, 1941
Msp20I	TGGCCA	1 cut(s)	3005
MspI	CCGG	4 cut(s)	51, 157, 1189, 2153
MspR9I	CCNGG	3 cut(s)	22, 35, 64
MssI	GTTTAAAC	1 cut(s)	2004
MunI	CAATTG	2 cut(s)	1392, 2583
Mva1269I	GAATGC	5 cut(s)	367, 424, 526, 968, 2124
MvaI	CCWGG	3 cut(s)	22, 35, 64
MvnI	CGCG	1 cut(s)	85
MwoI	GCNNNNNNNGC	9 cut(s)	187, 1003, 1034, 1565, 1574, 1964, 2218, 2330, 2524
NdeI	CATATG	1 cut(s)	2419
NdeII	GATC	7 cut(s)	379, 1030, 1435, 1684, 2145, 2362, 2608
NlaIV	GGNNCC	5 cut(s)	1343, 1569, 1706, 1968, 2466
NmeAIII	GCCGAG	1 cut(s)	1988
NmuCI	GTSAC	4 cut(s)	783, 1492, 1694, 2185
NsbI	TGCGCA	1 cut(s)	2211
NsiI	ATGCAT	3 cut(s)	2314, 2335, 2453
NspI	RCATGY	4 cut(s)	1327, 1467, 1544, 2498
NspV	TTCGAA	3 cut(s)	359, 1069, 2821
PaeI	GCATGC	1 cut(s)	1467
PagI	TCATGA	3 cut(s)	1447, 1942, 2605
PaqCI	CACCTGC	1 cut(s)	2068
PciSI	GCTCTTC	2 cut(s)	471, 1829
PctI	GAATGC	5 cut(s)	367, 424, 526, 968, 2124
PdmI	GAANNNNTTC	2 cut(s)	1653, 1782
PflFI	GACNNNGTC	1 cut(s)	2241
PkrI	GCNGC	7 cut(s)	571, 819, 1558, 1891, 2665, 2919, 2922
Ple19I	CGATCG	1 cut(s)	1687
PleI	GAGTC	8 cut(s)	251, 878, 913, 1132, 1445, 1732, 2412, 2610
PmeI	GTTTAAAC	1 cut(s)	2004
PpsI	GAGTC	8 cut(s)	251, 878, 913, 1132, 1445, 1732, 2412, 2610
Ppu21I	YACGTR	1 cut(s)	557
PsiI	TTATAA	1 cut(s)	1358
Psp1406I	AACGTT	2 cut(s)	871, 1867
Psp6I	CCWGG	3 cut(s)	20, 33, 62
PspGI	CCWGG	3 cut(s)	20, 33, 62
PspN4I	GGNNCC	5 cut(s)	1343, 1569, 1706, 1968, 2466
PspOMI	GGGCCC	1 cut(s)	1704
PspPI	GGNCC	4 cut(s)	1186, 1704, 1705, 3015
PstI	CTGCAG	1 cut(s)	183
PstNI	CAGNNNCTG	1 cut(s)	548
PsyI	GACNNNGTC	1 cut(s)	2241
PvuI	CGATCG	1 cut(s)	1687
RseI	CAYNNNNRTG	2 cut(s)	1221, 1941
SalI	GTCGAC	2 cut(s)	1015, 1740
SapI	GCTCTTC	2 cut(s)	471, 1829
SaqAI	TTAA	9 cut(s)	330, 611, 702, 1268, 2003, 2157, 2259, 2886, 3031
SatI	GCNGC	7 cut(s)	570, 818, 1557, 1890, 2664, 2918, 2921
Sau3AI	GATC	7 cut(s)	379, 1030, 1435, 1684, 2145, 2362, 2608
Sau96I	GGNCC	4 cut(s)	1186, 1704, 1705, 3015
ScaI	AGTACT	1 cut(s)	1002
SchI	GAGTC	8 cut(s)	251, 878, 914, 1133, 1445, 1733, 2413, 2611
ScrFI	CCNGG	3 cut(s)	22, 35, 64
SduI	GDGCHC	2 cut(s)	466, 1708
SexAI	ACCWGGT	1 cut(s)	20
SfaNI	GCATC	9 cut(s)	541, 609, 744, 1128, 1390, 2293, 2299, 2320, 2438
SfcI	CTRYAG	1 cut(s)	179
SfuI	TTCGAA	3 cut(s)	359, 1069, 2821
SinI	GGWCC	1 cut(s)	1186
SmiMI	CAYNNNNRTG	2 cut(s)	1221, 1941
SmlI	CTYRAG	2 cut(s)	573, 1895
SmoI	CTYRAG	2 cut(s)	573, 1895
SphI	GCATGC	1 cut(s)	1467
SsiI	CCGC	8 cut(s)	5, 83, 230, 422, 622, 653, 2221, 2917
SspI	AATATT	1 cut(s)	433
SspMI	CTAG	6 cut(s)	198, 1007, 1709, 2013, 2408, 2519
StyD4I	CCNGG	3 cut(s)	20, 33, 62
StyI	CCWWGG	2 cut(s)	1328, 2047
TaaI	ACNGT	9 cut(s)	222, 1146, 1774, 1839, 2194, 2349, 2806, 2867, 2976
TaiI	ACGT	7 cut(s)	559, 850, 874, 1855, 1870, 2242, 2881
TaqI	TCGA	9 cut(s)	353, 359, 875, 1016, 1069, 1438, 1734, 1741, 2821
TaqII	GACCGA	1 cut(s)	268
TatI	WGTACW	6 cut(s)	170, 1000, 2344, 2349, 2479, 2554
TauI	GCSGC	1 cut(s)	2920
Tru1I	TTAA	9 cut(s)	330, 611, 702, 1268, 2003, 2157, 2259, 2886, 3031
Tru9I	TTAA	9 cut(s)	330, 611, 702, 1268, 2003, 2157, 2259, 2886, 3031
TscAI	CASTG	3 cut(s)	1777, 2332, 2359
TseFI	GTSAC	4 cut(s)	783, 1492, 1694, 2185
TseI	GCWGC	6 cut(s)	569, 817, 1556, 1889, 2663, 2920
Tsp45I	GTSAC	4 cut(s)	783, 1492, 1694, 2185
TspGWI	ACGGA	3 cut(s)	854, 1456, 2989
TspRI	CASTG	3 cut(s)	1777, 2332, 2359
Tth111I	GACNNNGTC	1 cut(s)	2241
VpaK11BI	GGWCC	1 cut(s)	1186
XapI	RAATTY	6 cut(s)	634, 991, 1253, 1334, 2286, 2573
XceI	RCATGY	4 cut(s)	1327, 1467, 1544, 2498
XmiI	GTMKAC	2 cut(s)	1016, 1741
XmnI	GAANNNNTTC	2 cut(s)	1653, 1782
XspI	CTAG	6 cut(s)	198, 1007, 1709, 2013, 2408, 2519
ZrmI	AGTACT	1 cut(s)	1002
Zsp2I	ATGCAT	3 cut(s)	2314, 2335, 2453

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.