Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005055

Physical Location & Seq

Genomic Coordinates Reverse (-)

36344968 .. 36346717

Gene Structure 1750 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1271300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 573 bp

ATGGCAAACCCACAAACCACATCTACTGCGGCGGAAGTAAAGAACGTCTACTCGGTGTGGGCCCTCCCGCCGGATGACGTGGCCCCCAGGCTGAAGAAGCTGATGCAGGGCCTCCGTGCCGAGTTCGGTGGTCCACAGTTCGAGCCCCACATCACCGTGGTGGGGGCCATCAGCCTGACCCTGGACGACGCCCTCGCGAAGTTCCGATCGGCTTCTGAGGGCCTCAAGGCCTACGACGCCAAGGTTGATCGCGTGGCCACCGGCACCTTCTTTTACCAGTGCGTTTTCCTCCTCATAAATCCGACCCCTCAGGTGGTGGAAGCTAGTGCTCACTGCTCTGGCCATTTCGGTTTCAAAAGCTCAAACCCTTATATGCCACATTTGAGCATCCTTTACGGTGATCTGACCGACGAAGAGAAGAAGAAGGCGCAAGAAAAAGCTAGCACTCTCGACGACAGCATTGCCAGCCTGAGCTTCCCCGTTACTCGCCTTGCGTTGTACAAAACCGACACCGAAGATATAACTCTCAAGTCCTGGGAGAAGATTGCTGAGTGCACCCTCGAATCGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000394 GO:0003674 GO:0003824 GO:0004112 GO:0004113 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006388 GO:0006396 GO:0006399 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008033 GO:0008081 GO:0008150 GO:0008152 GO:0008380 GO:0009117 GO:0009187 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0019637 GO:0034470 GO:0034641 GO:0034660 GO:0042578 GO:0043170 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044464 GO:0046483 GO:0055086 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2_5_RNA_ligase2 CPDase

Protein Analysis

191

Amino Acids

20.84

Weight (kDa)

5.38

Isoelectric Point (pI)

34.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPDase	PF07823	18 - 157	1.9e-10	Cyclic phosphodiesterase-like protein
2_5_RNA_ligase2	PF13563	23 - 171	4.3e-11	2'-5' RNA ligase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005055)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18930
fragaria_vesca	FvH4_3g06750 FvH4_3g06750 FvH4_3g06750 FvH4_6g06860 FvH4_6g06860
malus_domestica	MD10G1271200.v1.1 MD10G1271300.v1.1
prunus_persica	Prupe.4G070300_v2.0.a1 Prupe.6G318900_v2.0.a1
pyrus_communis	pycom10g22700
rosa_chinensis	RchiOBHm_Chr3g0456341 RchiOBHm_Chr5g0012411
rosa_laevigata	RLG00000025311 RLG00000031911
rosa_multiflora	Rmu_co8444057.1_g000001 Rmu_sc0002607.1_g000022 Rmu_sc0018054.1_g000004
rosa_roxburghii	Rroxscaffold_1G00064260 Rroxscaffold_6G00422810
rosa_rugosa	Rorug05G0000900
rosa_samantha	Rh3AG072700 Rh3BG074800 Rh3CG074400 Rh5AG095400 Rh5AG499800 Rh5BG091600 Rh5DG091000
rosa_wichuraiana	Rw3G006240 Rw5G008340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	263
AccI	GTMKAC	1 cut(s)	48
AccII	CGCG	2 cut(s)	197, 252
AciI	CCGC	3 cut(s)	29, 32, 68
AcoI	YGGCCR	2 cut(s)	255, 340
AcuI	CTGAAG	1 cut(s)	113
AcyI	GRCGYC	2 cut(s)	189, 237
AfaI	GTAC	1 cut(s)	500
AfiI	CCNNNNNNNGG	4 cut(s)	70, 162, 181, 313
AgsI	TTSAA	1 cut(s)	355
AjiI	CACGTC	1 cut(s)	79
AjnI	CCWGG	3 cut(s)	86, 180, 533
AleI	CACNNNNGTG	1 cut(s)	158
AluBI	AGCT	5 cut(s)	100, 323, 360, 440, 474
AluI	AGCT	5 cut(s)	100, 323, 360, 440, 474
Alw21I	GWGCWC	2 cut(s)	331, 557
Alw44I	GTGCAC	1 cut(s)	553
AoxI	GGCC	8 cut(s)	60, 81, 109, 165, 220, 228, 255, 340
ApaI	GGGCCC	1 cut(s)	64
ApaLI	GTGCAC	1 cut(s)	553
AspLEI	GCGC	1 cut(s)	430
AspS9I	GGNCC	7 cut(s)	60, 61, 82, 109, 131, 165, 220
AsuHPI	GGTGA	2 cut(s)	145, 410
AsuNHI	GCTAGC	1 cut(s)	440
AvaII	GGWCC	1 cut(s)	131
AxyI	CCTNAGG	1 cut(s)	309
BaeGI	GKGCMC	2 cut(s)	64, 557
BalI	TGGCCA	2 cut(s)	257, 342
BanI	GGYRCC	1 cut(s)	263
BanII	GRGCYC	2 cut(s)	64, 147
Bbv12I	GWGCWC	2 cut(s)	331, 557
BccI	CCATC	1 cut(s)	176
BcgI	CGANNNNNNTGC	2 cut(s)	443, 477
BciT130I	CCWGG	3 cut(s)	88, 182, 535
BfaI	CTAG	2 cut(s)	324, 441
BisI	GCNGC	1 cut(s)	30
BlsI	GCNGC	1 cut(s)	31
Bme1390I	CCNGG	3 cut(s)	88, 182, 535
Bme18I	GGWCC	1 cut(s)	131
BmgBI	CACGTC	1 cut(s)	79
BmgT120I	GGNCC	7 cut(s)	60, 61, 82, 109, 131, 165, 220
BmiI	GGNNCC	4 cut(s)	62, 84, 166, 265
BmrFI	CCNGG	3 cut(s)	88, 182, 535
BmsI	GCATC	2 cut(s)	93, 396
BmtI	GCTAGC	1 cut(s)	444
Bpu10I	CCTNAGC	1 cut(s)	470
BpuEI	CTTGAG	2 cut(s)	209, 512
BsaHI	GRCGYC	2 cut(s)	189, 237
BsaJI	CCNNGG	5 cut(s)	86, 156, 180, 240, 534
Bsc4I	CCNNNNNNNGG	4 cut(s)	70, 162, 181, 313
Bse118I	RCCGGY	1 cut(s)	260
Bse1I	ACTGG	1 cut(s)	277
Bse21I	CCTNAGG	1 cut(s)	309
Bse3DI	GCAATG	1 cut(s)	459
BseBI	CCWGG	3 cut(s)	88, 182, 535
BseDI	CCNNGG	5 cut(s)	86, 156, 180, 240, 534
BseGI	GGATG	2 cut(s)	79, 387
BseLI	CCNNNNNNNGG	4 cut(s)	70, 162, 181, 313
BseMI	GCAATG	1 cut(s)	459
BseMII	CTCAG	4 cut(s)	207, 323, 461, 540
BseNI	ACTGG	1 cut(s)	277
BseRI	GAGGAG	1 cut(s)	281
BseSI	GKGCMC	2 cut(s)	64, 557
Bsh1236I	CGCG	2 cut(s)	197, 252
Bsh1285I	CGRYCG	1 cut(s)	209
BshFI	GGCC	8 cut(s)	62, 83, 111, 167, 222, 230, 257, 342
BshNI	GGYRCC	1 cut(s)	263
BsiEI	CGRYCG	1 cut(s)	209
BsiHKAI	GWGCWC	2 cut(s)	331, 557
BsiSI	CCGG	2 cut(s)	71, 261
BslI	CCNNNNNNNGG	4 cut(s)	70, 162, 181, 313
BsnI	GGCC	8 cut(s)	62, 83, 111, 167, 222, 230, 257, 342
Bsp120I	GGGCCC	1 cut(s)	60
Bsp1286I	GDGCHC	4 cut(s)	64, 147, 331, 557
Bsp1407I	TGTACA	1 cut(s)	498
Bsp143I	GATC	3 cut(s)	206, 247, 400
Bsp68I	TCGCGA	1 cut(s)	197
BspACI	CCGC	3 cut(s)	29, 32, 68
BspANI	GGCC	8 cut(s)	62, 83, 111, 167, 222, 230, 257, 342
BspCNI	CTCAG	4 cut(s)	208, 322, 462, 541
BspFNI	CGCG	2 cut(s)	197, 252
BspLI	GGNNCC	4 cut(s)	62, 84, 166, 265
BspOI	GCTAGC	1 cut(s)	444
BspT107I	GGYRCC	1 cut(s)	263
BsrDI	GCAATG	1 cut(s)	459
BsrFI	RCCGGY	1 cut(s)	260
BsrGI	TGTACA	1 cut(s)	498
BsrI	ACTGG	1 cut(s)	277
BssAI	RCCGGY	1 cut(s)	260
BssECI	CCNNGG	5 cut(s)	86, 156, 180, 240, 534
BssMI	GATC	3 cut(s)	206, 247, 400
BssNI	GRCGYC	2 cut(s)	189, 237
BssT1I	CCWWGG	1 cut(s)	240
Bst2UI	CCWGG	3 cut(s)	88, 182, 535
Bst4CI	ACNGT	3 cut(s)	138, 157, 398
Bst6I	CTCTTC	1 cut(s)	408
BstACI	GRCGYC	2 cut(s)	189, 237
BstAUI	TGTACA	1 cut(s)	498
BstC8I	GCNNGC	2 cut(s)	442, 466
BstDEI	CTNAG	4 cut(s)	216, 309, 470, 549
BstDSI	CCRYGG	1 cut(s)	156
BstF5I	GGATG	2 cut(s)	79, 387
BstFNI	CGCG	2 cut(s)	197, 252
BstHHI	GCGC	1 cut(s)	430
BstKTI	GATC	3 cut(s)	209, 250, 403
BstMBI	GATC	3 cut(s)	206, 247, 400
BstMCI	CGRYCG	1 cut(s)	209
BstMWI	GCNNNNNNNGC	3 cut(s)	97, 236, 465
BstNI	CCWGG	3 cut(s)	88, 182, 535
BstSCI	CCNGG	3 cut(s)	86, 180, 533
BstSLI	GKGCMC	2 cut(s)	64, 557
BstUI	CGCG	2 cut(s)	197, 252
Bsu36I	CCTNAGG	1 cut(s)	309
BsuRI	GGCC	8 cut(s)	62, 83, 111, 167, 222, 230, 257, 342
BtgI	CCRYGG	1 cut(s)	156
BtrI	CACGTC	1 cut(s)	79
BtsCI	GGATG	2 cut(s)	79, 387
BtsI	GCAGTG	1 cut(s)	331
BtsIMutI	CAGTG	2 cut(s)	284, 331
BtuMI	TCGCGA	1 cut(s)	197
Cac8I	GCNNGC	2 cut(s)	442, 466
CfoI	GCGC	1 cut(s)	430
Cfr10I	RCCGGY	1 cut(s)	260
Cfr13I	GGNCC	7 cut(s)	60, 61, 82, 109, 131, 165, 220
CseI	GACGC	2 cut(s)	197, 245
Csp6I	GTAC	1 cut(s)	499
CviQI	GTAC	1 cut(s)	499
DdeI	CTNAG	4 cut(s)	216, 309, 470, 549
DpnI	GATC	3 cut(s)	208, 249, 402
DpnII	GATC	3 cut(s)	206, 247, 400
EaeI	YGGCCR	2 cut(s)	255, 340
Eam1104I	CTCTTC	1 cut(s)	408
EarI	CTCTTC	1 cut(s)	408
EciI	GGCGGA	1 cut(s)	47
Eco130I	CCWWGG	1 cut(s)	240
Eco147I	AGGCCT	1 cut(s)	230
Eco24I	GRGCYC	2 cut(s)	64, 147
Eco47I	GGWCC	1 cut(s)	131
Eco57I	CTGAAG	1 cut(s)	113
Eco81I	CCTNAGG	1 cut(s)	309
EcoO109I	RGGNCCY	3 cut(s)	61, 109, 220
EcoRII	CCWGG	3 cut(s)	86, 180, 533
EcoT14I	CCWWGG	1 cut(s)	240
EcoT38I	GRGCYC	2 cut(s)	64, 147
ErhI	CCWWGG	1 cut(s)	240
FaiI	YATR	4 cut(s)	296, 372, 374, 521
FauI	CCCGC	1 cut(s)	75
FblI	GTMKAC	1 cut(s)	48
Fnu4HI	GCNGC	1 cut(s)	30
FokI	GGATG	2 cut(s)	86, 374
FriOI	GRGCYC	2 cut(s)	64, 147
Fsp4HI	GCNGC	1 cut(s)	30
FspBI	CTAG	2 cut(s)	324, 441
GlaI	GCGC	1 cut(s)	429
GluI	GCNGC	1 cut(s)	30
HaeIII	GGCC	8 cut(s)	62, 83, 111, 167, 222, 230, 257, 342
HapII	CCGG	2 cut(s)	71, 261
HgaI	GACGC	2 cut(s)	197, 245
HhaI	GCGC	1 cut(s)	430
Hin1I	GRCGYC	2 cut(s)	189, 237
Hin6I	GCGC	1 cut(s)	428
HinP1I	GCGC	1 cut(s)	428
HinfI	GANTC	1 cut(s)	563
HpaII	CCGG	2 cut(s)	71, 261
HphI	GGTGA	2 cut(s)	145, 410
Hpy166II	GTNNAC	3 cut(s)	49, 134, 555
Hpy188I	TCNGA	4 cut(s)	206, 217, 303, 405
Hpy188III	TCNNGA	2 cut(s)	196, 449
Hpy8I	GTNNAC	3 cut(s)	49, 134, 555
Hpy99I	CGWCG	4 cut(s)	191, 239, 413, 455
HpyAV	CCTTC	2 cut(s)	277, 418
HpyCH4III	ACNGT	3 cut(s)	138, 157, 398
HpyCH4IV	ACGT	2 cut(s)	45, 78
HpyCH4V	TGCA	2 cut(s)	106, 555
HpyF10VI	GCNNNNNNNGC	3 cut(s)	97, 236, 465
HpyF3I	CTNAG	4 cut(s)	216, 309, 470, 549
HpySE526I	ACGT	2 cut(s)	45, 78
Hsp92I	GRCGYC	2 cut(s)	189, 237
HspAI	GCGC	1 cut(s)	428
Kzo9I	GATC	3 cut(s)	206, 247, 400
LweI	GCATC	2 cut(s)	93, 396
MaeI	CTAG	2 cut(s)	324, 441
MaeII	ACGT	2 cut(s)	45, 78
MaeIII	GTNAC	1 cut(s)	481
MalI	GATC	3 cut(s)	208, 249, 402
MboI	GATC	3 cut(s)	206, 247, 400
MboII	GAAGA	6 cut(s)	106, 425, 430, 433, 527, 553
MhlI	GDGCHC	4 cut(s)	64, 147, 331, 557
MlsI	TGGCCA	2 cut(s)	257, 342
MluCI	AATT	1 cut(s)	568
MluNI	TGGCCA	2 cut(s)	257, 342
MmeI	TCCRAC	1 cut(s)	326
MnlI	CCTC	9 cut(s)	74, 122, 203, 211, 233, 299, 302, 318, 569
Mox20I	TGGCCA	2 cut(s)	257, 342
MscI	TGGCCA	2 cut(s)	257, 342
MslI	CAYNNNNRTG	2 cut(s)	155, 158
Msp20I	TGGCCA	2 cut(s)	257, 342
MspI	CCGG	2 cut(s)	71, 261
MspR9I	CCNGG	3 cut(s)	88, 182, 535
MvaI	CCWGG	3 cut(s)	88, 182, 535
MvnI	CGCG	2 cut(s)	197, 252
MwoI	GCNNNNNNNGC	3 cut(s)	97, 236, 465
NdeII	GATC	3 cut(s)	206, 247, 400
NheI	GCTAGC	1 cut(s)	440
NlaIV	GGNNCC	4 cut(s)	62, 84, 166, 265
NmeAIII	GCCGAG	1 cut(s)	145
NruI	TCGCGA	1 cut(s)	197
OliI	CACNNNNGTG	1 cut(s)	158
PceI	AGGCCT	1 cut(s)	230
PfeI	GAWTC	1 cut(s)	563
PkrI	GCNGC	1 cut(s)	31
Ple19I	CGATCG	1 cut(s)	209
Psp6I	CCWGG	3 cut(s)	86, 180, 533
PspGI	CCWGG	3 cut(s)	86, 180, 533
PspN4I	GGNNCC	4 cut(s)	62, 84, 166, 265
PspOMI	GGGCCC	1 cut(s)	60
PspPI	GGNCC	7 cut(s)	60, 61, 82, 109, 131, 165, 220
PvuI	CGATCG	1 cut(s)	209
RruI	TCGCGA	1 cut(s)	197
RsaI	GTAC	1 cut(s)	500
RsaNI	GTAC	1 cut(s)	499
RseI	CAYNNNNRTG	2 cut(s)	155, 158
SatI	GCNGC	1 cut(s)	30
Sau3AI	GATC	3 cut(s)	206, 247, 400
Sau96I	GGNCC	7 cut(s)	60, 61, 82, 109, 131, 165, 220
ScrFI	CCNGG	3 cut(s)	88, 182, 535
SduI	GDGCHC	4 cut(s)	64, 147, 331, 557
SfaNI	GCATC	2 cut(s)	93, 396
SinI	GGWCC	1 cut(s)	131
SmiMI	CAYNNNNRTG	2 cut(s)	155, 158
SmlI	CTYRAG	2 cut(s)	224, 527
SmoI	CTYRAG	2 cut(s)	224, 527
Sse9I	AATT	1 cut(s)	568
SseBI	AGGCCT	1 cut(s)	230
SsiI	CCGC	3 cut(s)	29, 32, 68
SspMI	CTAG	2 cut(s)	324, 441
StuI	AGGCCT	1 cut(s)	230
StyD4I	CCNGG	3 cut(s)	86, 180, 533
StyI	CCWWGG	1 cut(s)	240
TaaI	ACNGT	3 cut(s)	138, 157, 398
TaiI	ACGT	2 cut(s)	48, 81
TaqI	TCGA	4 cut(s)	141, 450, 561, 566
TaqII	GACCGA	1 cut(s)	422
TasI	AATT	1 cut(s)	568
TatI	WGTACW	1 cut(s)	498
TauI	GCSGC	1 cut(s)	32
TfiI	GAWTC	1 cut(s)	563
TscAI	CASTG	2 cut(s)	284, 338
TspGWI	ACGGA	1 cut(s)	104
TspRI	CASTG	2 cut(s)	284, 338
VneI	GTGCAC	1 cut(s)	553
VpaK11BI	GGWCC	1 cut(s)	131
XmiI	GTMKAC	1 cut(s)	48
XspI	CTAG	2 cut(s)	324, 441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.