MD10G1327500.v1.1

Transcriptional repressor, ovate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011981
Physical Location & Seq
Forward (+)
40717984 .. 40718490
507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1327500.v1.1.491

Sequence Viewer

Length: 507 bp
ATGTCTAAAACGAAAGCCTCCTCCAAGCAGCAACAGAGAGTATGCAGGGCATTGTGTTGCAGCAGCTGCCGGCTTAGCGTGTCTTCGTCTAGCTCGGAGGAAGTAGCGAGCTCTAGTTCCGATAGGTACCCTTCGATATCAAGCCTATCGCATGCCATGGTTCAAGAGAGACTCGACCAGATGATCAGGGAAAGGCAGGAGGGATCGAGGCACGTTGATCATCGGAGAAGAAAGCAGAGAGCTGGTGACGATCATGATCAACTTCAAGCAGGAGGAGGGACTAAATTTGTTGTAATGGTTGCAATGGAGAAGTGTTCTTATGATCCGAGGGAGGATTTCAGGGAGTCCATGGCGGCGATGATAGTGGCAAACCGGATAGAAGAGCCTAAGGATCTTCGTAGCCTCTTGAATTATTACGTTTCGATGAATTCCGATGAGTATCGTGGAATTATACTTGAGGTGTTTCATCAAGTTTGCTCTGATTTATTCTTATGTAAATTCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ovate

Protein Analysis

169

Amino Acids

19.21

Weight (kDa)

8.17

Isoelectric Point (pI)

61.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 106 - 163 5.2e-21 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04025
fragaria_vesca FvH4_3g01350
malus_domestica MD05G1352900.v1.1 MD10G1327500.v1.1
prunus_persica Prupe.4G014500_v2.0.a1
pyrus_communis pycom05g32020 pycom05g32100 pycom17g07250
rosa_chinensis RchiOBHm_Chr5g0001971
rosa_laevigata RLG00000030985
rosa_multiflora Rmu_sc0000378.1_g000017 Rmu_sc0006008.1_g000006
rosa_roxburghii Rroxscaffold_1G00071360 Rroxscaffold_1G00074380
rosa_rugosa Rorug04G0394100
rosa_samantha Rh5BG018800 Rh5CG016500 Rh5DG017100
rosa_wichuraiana Rw5G001490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 126
AciI CCGC 1 cut(s) 353
AclWI GGATC 3 cut(s) 211, 317, 399
AcsI RAATTY 3 cut(s) 284, 427, 497
AfaI GTAC 1 cut(s) 128
AgsI TTSAA 3 cut(s) 164, 266, 409
AluBI AGCT 4 cut(s) 66, 93, 111, 242
AluI AGCT 4 cut(s) 66, 93, 111, 242
Alw21I GWGCWC 1 cut(s) 113
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 3 cut(s) 211, 317, 399
AlwNI CAGNNNCTG 1 cut(s) 66
ApeKI GCWGC 4 cut(s) 28, 60, 63, 66
ApoI RAATTY 3 cut(s) 284, 427, 497
Asp718I GGTACC 1 cut(s) 126
AsuHPI GGTGA 1 cut(s) 257
AxyI CCTNAGG 1 cut(s) 387
BanI GGYRCC 1 cut(s) 126
BanII GRGCYC 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 113
BbvI GCAGC 4 cut(s) 40, 53, 72, 75
BclI TGATCA 3 cut(s) 183, 217, 256
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 2 cut(s) 90, 114
BisI GCNGC 5 cut(s) 29, 61, 64, 67, 354
BlpI GCTNAGC 1 cut(s) 74
BlsI GCNGC 5 cut(s) 30, 62, 65, 68, 355
BmiI GGNNCC 1 cut(s) 128
BpiI GAAGAC 1 cut(s) 75
Bpu1102I GCTNAGC 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 476
BsaBI GATNNNNATC 2 cut(s) 255, 438
BsaJI CCNNGG 3 cut(s) 156, 326, 348
BsaWI WCCGGW 1 cut(s) 372
Bse118I RCCGGY 1 cut(s) 69
Bse21I CCTNAGG 1 cut(s) 387
Bse3DI GCAATG 1 cut(s) 309
Bse8I GATNNNNATC 2 cut(s) 255, 438
BseDI CCNNGG 3 cut(s) 156, 326, 348
BseJI GATNNNNATC 2 cut(s) 255, 438
BseMI GCAATG 1 cut(s) 309
BseRI GAGGAG 2 cut(s) 10, 288
BseXI GCAGC 4 cut(s) 40, 53, 72, 75
BshNI GGYRCC 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 113
BsiSI CCGG 2 cut(s) 70, 373
BslFI GGGAC 1 cut(s) 292
BsmAI GTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 113
Bsp143I GATC 7 cut(s) 183, 203, 217, 250, 256, 322, 391
Bsp1720I GCTNAGC 1 cut(s) 74
Bsp19I CCATGG 2 cut(s) 156, 348
BspACI CCGC 1 cut(s) 353
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 128
BspPI GGATC 3 cut(s) 211, 317, 399
BspQI GCTCTTC 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 126
BsrDI GCAATG 1 cut(s) 309
BsrFI RCCGGY 1 cut(s) 69
BssAI RCCGGY 1 cut(s) 69
BssECI CCNNGG 3 cut(s) 156, 326, 348
BssMI GATC 7 cut(s) 183, 203, 217, 250, 256, 322, 391
BssT1I CCWWGG 2 cut(s) 156, 348
Bst6I CTCTTC 1 cut(s) 375
BstAPI GCANNNNNTGC 1 cut(s) 66
BstC8I GCNNGC 3 cut(s) 71, 109, 153
BstDEI CTNAG 2 cut(s) 74, 387
BstDSI CCRYGG 2 cut(s) 156, 348
BstKTI GATC 7 cut(s) 186, 206, 220, 253, 259, 325, 394
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 7 cut(s) 183, 203, 217, 250, 256, 322, 391
BstMWI GCNNNNNNNGC 2 cut(s) 66, 75
BstNSI RCATGY 1 cut(s) 155
BstV1I GCAGC 4 cut(s) 40, 53, 72, 75
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
Bsu36I CCTNAGG 1 cut(s) 387
BtgI CCRYGG 2 cut(s) 156, 348
BtgZI GCGATG 1 cut(s) 371
Cac8I GCNNGC 3 cut(s) 71, 109, 153
CaiI CAGNNNCTG 1 cut(s) 66
CciI TCATGA 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 69
Csp6I GTAC 1 cut(s) 127
CviAII CATG 4 cut(s) 152, 157, 254, 349
CviJI RGCY 9 cut(s) 17, 66, 73, 93, 111, 144, 242, 385, 402
CviKI_1 RGCY 9 cut(s) 17, 66, 73, 93, 111, 144, 242, 385, 402
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 2 cut(s) 74, 387
DpnI GATC 7 cut(s) 185, 205, 219, 252, 258, 324, 393
DpnII GATC 7 cut(s) 183, 203, 217, 250, 256, 322, 391
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Ecl136II GAGCTC 1 cut(s) 111
Eco130I CCWWGG 2 cut(s) 156, 348
Eco24I GRGCYC 1 cut(s) 113
Eco32I GATATC 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 111
Eco81I CCTNAGG 1 cut(s) 387
EcoICRI GAGCTC 1 cut(s) 111
EcoRI GAATTC 1 cut(s) 427
EcoRV GATATC 1 cut(s) 138
EcoT14I CCWWGG 2 cut(s) 156, 348
EcoT38I GRGCYC 1 cut(s) 113
ErhI CCWWGG 2 cut(s) 156, 348
FaeI CATG 4 cut(s) 155, 160, 257, 352
FaiI YATR 8 cut(s) 43, 153, 158, 255, 321, 350, 452, 493
FaqI GGGAC 1 cut(s) 292
FatI CATG 4 cut(s) 151, 156, 253, 348
FbaI TGATCA 3 cut(s) 183, 217, 256
Fnu4HI GCNGC 5 cut(s) 29, 61, 64, 67, 354
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 5 cut(s) 29, 61, 64, 67, 354
FspBI CTAG 2 cut(s) 90, 114
GluI GCNGC 5 cut(s) 29, 61, 64, 67, 354
HapII CCGG 2 cut(s) 70, 373
Hin1II CATG 4 cut(s) 155, 160, 257, 352
HinfI GANTC 2 cut(s) 171, 344
HpaII CCGG 2 cut(s) 70, 373
HphI GGTGA 1 cut(s) 257
Hpy188I TCNGA 6 cut(s) 97, 121, 225, 327, 433, 481
Hpy188III TCNNGA 3 cut(s) 164, 254, 406
HpyAV CCTTC 1 cut(s) 141
HpyCH4IV ACGT 2 cut(s) 213, 417
HpyCH4V TGCA 3 cut(s) 45, 60, 302
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 75
HpyF3I CTNAG 2 cut(s) 74, 387
HpySE526I ACGT 2 cut(s) 213, 417
Hsp92II CATG 4 cut(s) 155, 160, 257, 352
KpnI GGTACC 1 cut(s) 130
KroI GCCGGC 1 cut(s) 69
KroNI GCCGGC 1 cut(s) 71
Ksp22I TGATCA 3 cut(s) 183, 217, 256
Kzo9I GATC 7 cut(s) 183, 203, 217, 250, 256, 322, 391
LguI GCTCTTC 1 cut(s) 375
LpnPI CCDG 9 cut(s) 31, 83, 172, 182, 191, 228, 255, 325, 386
Lsp1109I GCAGC 4 cut(s) 40, 53, 72, 75
MaeI CTAG 2 cut(s) 90, 114
MaeII ACGT 2 cut(s) 213, 417
MaeIII GTNAC 1 cut(s) 245
MalI GATC 7 cut(s) 185, 205, 219, 252, 258, 324, 393
MboI GATC 7 cut(s) 183, 203, 217, 250, 256, 322, 391
MboII GAAGA 4 cut(s) 75, 240, 386, 392
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 1 cut(s) 113
MluCI AATT 5 cut(s) 284, 409, 427, 447, 497
MlyI GAGTC 2 cut(s) 165, 353
MroNI GCCGGC 1 cut(s) 69
MseI TTAA 1 cut(s) 505
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 2 cut(s) 70, 373
MwoI GCNNNNNNNGC 2 cut(s) 66, 75
NaeI GCCGGC 1 cut(s) 71
NcoI CCATGG 2 cut(s) 156, 348
NdeII GATC 7 cut(s) 183, 203, 217, 250, 256, 322, 391
NgoMIV GCCGGC 1 cut(s) 69
NlaIII CATG 4 cut(s) 155, 160, 257, 352
NlaIV GGNNCC 1 cut(s) 128
NmuCI GTSAC 1 cut(s) 245
NspI RCATGY 1 cut(s) 155
PaeI GCATGC 1 cut(s) 155
PagI TCATGA 1 cut(s) 253
PciSI GCTCTTC 1 cut(s) 375
PdiI GCCGGC 1 cut(s) 71
PkrI GCNGC 5 cut(s) 30, 62, 65, 68, 355
PleI GAGTC 2 cut(s) 165, 352
PpsI GAGTC 2 cut(s) 165, 352
Psp124BI GAGCTC 1 cut(s) 113
PspN4I GGNNCC 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 66
PsuI RGATCY 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 66
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
SacI GAGCTC 1 cut(s) 113
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 1 cut(s) 505
SatI GCNGC 5 cut(s) 29, 61, 64, 67, 354
Sau3AI GATC 7 cut(s) 183, 203, 217, 250, 256, 322, 391
SchI GAGTC 2 cut(s) 165, 353
SduI GDGCHC 1 cut(s) 113
SetI ASST 8 cut(s) 68, 95, 113, 128, 216, 244, 420, 462
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
SphI GCATGC 1 cut(s) 155
Sse9I AATT 5 cut(s) 284, 409, 427, 447, 497
SsiI CCGC 1 cut(s) 353
SspMI CTAG 2 cut(s) 90, 114
SstI GAGCTC 1 cut(s) 113
StyI CCWWGG 2 cut(s) 156, 348
TaiI ACGT 2 cut(s) 216, 420
TaqI TCGA 4 cut(s) 134, 174, 206, 422
TasI AATT 5 cut(s) 284, 409, 427, 447, 497
TauI GCSGC 1 cut(s) 356
Tru1I TTAA 1 cut(s) 505
Tru9I TTAA 1 cut(s) 505
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 4 cut(s) 28, 60, 63, 66
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 2 cut(s) 440, 455
XapI RAATTY 3 cut(s) 284, 427, 497
XceI RCATGY 1 cut(s) 155
XspI CTAG 2 cut(s) 90, 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.