Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 568 |
| AccBSI | CCGCTC | 1 cut(s) | 80 |
| AccII | CGCG | 1 cut(s) | 344 |
| AciI | CCGC | 6 cut(s) | 48, 75, 78, 206, 332, 344 |
| AclWI | GGATC | 1 cut(s) | 605 |
| AfaI | GTAC | 2 cut(s) | 35, 233 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 95, 573 |
| AgsI | TTSAA | 1 cut(s) | 256 |
| AjnI | CCWGG | 1 cut(s) | 171 |
| AluBI | AGCT | 8 cut(s) | 43, 131, 186, 302, 313, 451, 620, 658 |
| AluI | AGCT | 8 cut(s) | 43, 131, 186, 302, 313, 451, 620, 658 |
| Alw21I | GWGCWC | 2 cut(s) | 45, 622 |
| Alw26I | GTCTC | 2 cut(s) | 434, 463 |
| AlwI | GGATC | 1 cut(s) | 605 |
| ApeKI | GCWGC | 3 cut(s) | 106, 190, 211 |
| AspS9I | GGNCC | 1 cut(s) | 169 |
| AsuC2I | CCSGG | 2 cut(s) | 61, 195 |
| AsuHPI | GGTGA | 1 cut(s) | 273 |
| AsuII | TTCGAA | 1 cut(s) | 15 |
| AvaII | GGWCC | 1 cut(s) | 169 |
| BanII | GRGCYC | 2 cut(s) | 45, 622 |
| BauI | CACGAG | 1 cut(s) | 303 |
| BbsI | GAAGAC | 1 cut(s) | 4 |
| Bbv12I | GWGCWC | 2 cut(s) | 45, 622 |
| BbvI | GCAGC | 3 cut(s) | 118, 198, 202 |
| BccI | CCATC | 3 cut(s) | 143, 322, 545 |
| BciT130I | CCWGG | 1 cut(s) | 173 |
| BclI | TGATCA | 1 cut(s) | 355 |
| BcnI | CCSGG | 2 cut(s) | 61, 195 |
| BcoDI | GTCTC | 2 cut(s) | 434, 463 |
| BisI | GCNGC | 5 cut(s) | 75, 78, 107, 191, 212 |
| BlsI | GCNGC | 5 cut(s) | 76, 79, 108, 192, 213 |
| BmcAI | AGTACT | 1 cut(s) | 35 |
| Bme1390I | CCNGG | 3 cut(s) | 61, 173, 195 |
| Bme18I | GGWCC | 1 cut(s) | 169 |
| BmgT120I | GGNCC | 1 cut(s) | 169 |
| BmrFI | CCNGG | 3 cut(s) | 61, 173, 195 |
| BmsI | GCATC | 3 cut(s) | 133, 487, 523 |
| BpiI | GAAGAC | 1 cut(s) | 4 |
| Bpu14I | TTCGAA | 1 cut(s) | 15 |
| BpuMI | CCSGG | 2 cut(s) | 61, 195 |
| BsaBI | GATNNNNATC | 1 cut(s) | 477 |
| BsaI | GGTCTC | 1 cut(s) | 434 |
| BsaJI | CCNNGG | 2 cut(s) | 94, 194 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 609, 639 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 95, 573 |
| Bse3DI | GCAATG | 1 cut(s) | 381 |
| Bse8I | GATNNNNATC | 1 cut(s) | 477 |
| BseBI | CCWGG | 1 cut(s) | 173 |
| BseDI | CCNNGG | 2 cut(s) | 94, 194 |
| BseGI | GGATG | 3 cut(s) | 145, 333, 585 |
| BseJI | GATNNNNATC | 1 cut(s) | 477 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 95, 573 |
| BseMI | GCAATG | 1 cut(s) | 381 |
| BseMII | CTCAG | 3 cut(s) | 111, 408, 621 |
| BseXI | GCAGC | 3 cut(s) | 118, 198, 202 |
| Bsh1236I | CGCG | 1 cut(s) | 344 |
| BsiHKAI | GWGCWC | 2 cut(s) | 45, 622 |
| BsiSI | CCGG | 2 cut(s) | 61, 194 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 95, 573 |
| BsmAI | GTCTC | 2 cut(s) | 434, 463 |
| Bso31I | GGTCTC | 1 cut(s) | 434 |
| Bsp119I | TTCGAA | 1 cut(s) | 15 |
| Bsp1286I | GDGCHC | 2 cut(s) | 45, 622 |
| Bsp143I | GATC | 4 cut(s) | 241, 264, 355, 597 |
| BspACI | CCGC | 6 cut(s) | 48, 75, 78, 206, 332, 344 |
| BspCNI | CTCAG | 3 cut(s) | 112, 409, 620 |
| BspFNI | CGCG | 1 cut(s) | 344 |
| BspPI | GGATC | 1 cut(s) | 605 |
| BspQI | GCTCTTC | 2 cut(s) | 87, 441 |
| BspT104I | TTCGAA | 1 cut(s) | 15 |
| BspTNI | GGTCTC | 1 cut(s) | 434 |
| BsrBI | CCGCTC | 1 cut(s) | 80 |
| BsrDI | GCAATG | 1 cut(s) | 381 |
| BssECI | CCNNGG | 2 cut(s) | 94, 194 |
| BssMI | GATC | 4 cut(s) | 241, 264, 355, 597 |
| BssSI | CACGAG | 1 cut(s) | 303 |
| BssT1I | CCWWGG | 1 cut(s) | 94 |
| Bst2BI | CACGAG | 1 cut(s) | 303 |
| Bst2UI | CCWGG | 1 cut(s) | 173 |
| Bst4CI | ACNGT | 1 cut(s) | 38 |
| Bst6I | CTCTTC | 3 cut(s) | 87, 251, 441 |
| BstBI | TTCGAA | 1 cut(s) | 15 |
| BstC8I | GCNNGC | 3 cut(s) | 188, 216, 342 |
| BstDEI | CTNAG | 3 cut(s) | 120, 417, 607 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 93 |
| BstF5I | GGATG | 3 cut(s) | 145, 333, 585 |
| BstFNI | CGCG | 1 cut(s) | 344 |
| BstKTI | GATC | 4 cut(s) | 244, 267, 358, 600 |
| BstMAI | GTCTC | 2 cut(s) | 434, 463 |
| BstMBI | GATC | 4 cut(s) | 241, 264, 355, 597 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 106, 484 |
| BstNI | CCWGG | 1 cut(s) | 173 |
| BstSCI | CCNGG | 3 cut(s) | 59, 171, 193 |
| BstUI | CGCG | 1 cut(s) | 344 |
| BstV1I | GCAGC | 3 cut(s) | 118, 198, 202 |
| BstV2I | GAAGAC | 1 cut(s) | 4 |
| BtsCI | GGATG | 3 cut(s) | 145, 333, 585 |
| Cac8I | GCNNGC | 3 cut(s) | 188, 216, 342 |
| Cfr13I | GGNCC | 1 cut(s) | 169 |
| Csp6I | GTAC | 2 cut(s) | 34, 232 |
| CviAII | CATG | 1 cut(s) | 568 |
| CviQI | GTAC | 2 cut(s) | 34, 232 |
| DdeI | CTNAG | 3 cut(s) | 120, 417, 607 |
| DpnI | GATC | 4 cut(s) | 243, 266, 357, 599 |
| DpnII | GATC | 4 cut(s) | 241, 264, 355, 597 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 568 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 568 |
| Eam1104I | CTCTTC | 3 cut(s) | 87, 251, 441 |
| EarI | CTCTTC | 3 cut(s) | 87, 251, 441 |
| EciI | GGCGGA | 2 cut(s) | 37, 347 |
| Ecl136II | GAGCTC | 2 cut(s) | 43, 620 |
| Eco130I | CCWWGG | 1 cut(s) | 94 |
| Eco24I | GRGCYC | 2 cut(s) | 45, 622 |
| Eco31I | GGTCTC | 1 cut(s) | 434 |
| Eco47I | GGWCC | 1 cut(s) | 169 |
| Eco53kI | GAGCTC | 2 cut(s) | 43, 620 |
| EcoICRI | GAGCTC | 2 cut(s) | 43, 620 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 93 |
| EcoRII | CCWGG | 1 cut(s) | 171 |
| EcoT14I | CCWWGG | 1 cut(s) | 94 |
| EcoT38I | GRGCYC | 2 cut(s) | 45, 622 |
| ErhI | CCWWGG | 1 cut(s) | 94 |
| FaeI | CATG | 1 cut(s) | 571 |
| FaiI | YATR | 6 cut(s) | 338, 434, 510, 569, 591, 647 |
| FatI | CATG | 1 cut(s) | 567 |
| FbaI | TGATCA | 1 cut(s) | 355 |
| Fnu4HI | GCNGC | 5 cut(s) | 75, 78, 107, 191, 212 |
| FokI | GGATG | 3 cut(s) | 152, 340, 592 |
| FriOI | GRGCYC | 2 cut(s) | 45, 622 |
| Fsp4HI | GCNGC | 5 cut(s) | 75, 78, 107, 191, 212 |
| GluI | GCNGC | 5 cut(s) | 75, 78, 107, 191, 212 |
| HapII | CCGG | 2 cut(s) | 61, 194 |
| Hin1II | CATG | 1 cut(s) | 571 |
| HindIII | AAGCTT | 1 cut(s) | 184 |
| HinfI | GANTC | 3 cut(s) | 157, 273, 320 |
| HpaII | CCGG | 2 cut(s) | 61, 194 |
| HphI | GGTGA | 1 cut(s) | 273 |
| Hpy188I | TCNGA | 4 cut(s) | 121, 162, 241, 355 |
| Hpy188III | TCNNGA | 3 cut(s) | 89, 305, 559 |
| Hpy99I | CGWCG | 1 cut(s) | 605 |
| HpyAV | CCTTC | 1 cut(s) | 11 |
| HpyCH4III | ACNGT | 1 cut(s) | 38 |
| HpyCH4IV | ACGT | 1 cut(s) | 28 |
| HpyCH4V | TGCA | 5 cut(s) | 190, 218, 340, 478, 632 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 106, 484 |
| HpyF3I | CTNAG | 3 cut(s) | 120, 417, 607 |
| HpySE526I | ACGT | 1 cut(s) | 28 |
| Hsp92II | CATG | 1 cut(s) | 571 |
| Ksp22I | TGATCA | 1 cut(s) | 355 |
| Kzo9I | GATC | 4 cut(s) | 241, 264, 355, 597 |
| LguI | GCTCTTC | 2 cut(s) | 87, 441 |
| LmnI | GCTCC | 1 cut(s) | 617 |
| LpnPI | CCDG | 7 cut(s) | 74, 102, 117, 158, 185, 207, 309 |
| Lsp1109I | GCAGC | 3 cut(s) | 118, 198, 202 |
| LweI | GCATC | 3 cut(s) | 133, 487, 523 |
| MaeII | ACGT | 1 cut(s) | 28 |
| MaeIII | GTNAC | 3 cut(s) | 68, 404, 570 |
| MalI | GATC | 4 cut(s) | 243, 266, 357, 599 |
| MbiI | CCGCTC | 1 cut(s) | 80 |
| MboI | GATC | 4 cut(s) | 241, 264, 355, 597 |
| MboII | GAAGA | 7 cut(s) | 4, 74, 77, 268, 342, 438, 458 |
| MhlI | GDGCHC | 2 cut(s) | 45, 622 |
| MluCI | AATT | 1 cut(s) | 411 |
| MlyI | GAGTC | 1 cut(s) | 329 |
| MnlI | CCTC | 5 cut(s) | 65, 105, 219, 252, 570 |
| MseI | TTAA | 3 cut(s) | 392, 654, 667 |
| MspI | CCGG | 2 cut(s) | 61, 194 |
| MspR9I | CCNGG | 3 cut(s) | 61, 173, 195 |
| MvaI | CCWGG | 1 cut(s) | 173 |
| MvnI | CGCG | 1 cut(s) | 344 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 106, 484 |
| NciI | CCSGG | 2 cut(s) | 61, 195 |
| NdeII | GATC | 4 cut(s) | 241, 264, 355, 597 |
| NlaIII | CATG | 1 cut(s) | 571 |
| NmuCI | GTSAC | 2 cut(s) | 404, 570 |
| NspV | TTCGAA | 1 cut(s) | 15 |
| PciSI | GCTCTTC | 2 cut(s) | 87, 441 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 21 |
| PfeI | GAWTC | 2 cut(s) | 157, 273 |
| PfoI | TCCNGGA | 1 cut(s) | 59 |
| PkrI | GCNGC | 5 cut(s) | 76, 79, 108, 192, 213 |
| PleI | GAGTC | 1 cut(s) | 328 |
| PpsI | GAGTC | 1 cut(s) | 328 |
| Psp124BI | GAGCTC | 2 cut(s) | 45, 622 |
| Psp6I | CCWGG | 1 cut(s) | 171 |
| PspGI | CCWGG | 1 cut(s) | 171 |
| PspPI | GGNCC | 1 cut(s) | 169 |
| RsaI | GTAC | 2 cut(s) | 35, 233 |
| RsaNI | GTAC | 2 cut(s) | 34, 232 |
| SacI | GAGCTC | 2 cut(s) | 45, 622 |
| SapI | GCTCTTC | 2 cut(s) | 87, 441 |
| SaqAI | TTAA | 3 cut(s) | 392, 654, 667 |
| SatI | GCNGC | 5 cut(s) | 75, 78, 107, 191, 212 |
| Sau3AI | GATC | 4 cut(s) | 241, 264, 355, 597 |
| Sau96I | GGNCC | 1 cut(s) | 169 |
| ScaI | AGTACT | 1 cut(s) | 35 |
| SchI | GAGTC | 1 cut(s) | 329 |
| ScrFI | CCNGG | 3 cut(s) | 61, 173, 195 |
| SduI | GDGCHC | 2 cut(s) | 45, 622 |
| SfaNI | GCATC | 3 cut(s) | 133, 487, 523 |
| SfuI | TTCGAA | 1 cut(s) | 15 |
| SinI | GGWCC | 1 cut(s) | 169 |
| Sse9I | AATT | 1 cut(s) | 411 |
| SsiI | CCGC | 6 cut(s) | 48, 75, 78, 206, 332, 344 |
| SstI | GAGCTC | 2 cut(s) | 45, 622 |
| StyD4I | CCNGG | 3 cut(s) | 59, 171, 193 |
| StyI | CCWWGG | 1 cut(s) | 94 |
| TaaI | ACNGT | 1 cut(s) | 38 |
| TaiI | ACGT | 1 cut(s) | 31 |
| TaqI | TCGA | 2 cut(s) | 15, 540 |
| TaqII | GACCGA | 1 cut(s) | 458 |
| TasI | AATT | 1 cut(s) | 411 |
| TatI | WGTACW | 2 cut(s) | 33, 231 |
| TauI | GCSGC | 2 cut(s) | 77, 80 |
| TfiI | GAWTC | 2 cut(s) | 157, 273 |
| Tru1I | TTAA | 3 cut(s) | 392, 654, 667 |
| Tru9I | TTAA | 3 cut(s) | 392, 654, 667 |
| TseFI | GTSAC | 2 cut(s) | 404, 570 |
| TseI | GCWGC | 3 cut(s) | 106, 190, 211 |
| Tsp45I | GTSAC | 2 cut(s) | 404, 570 |
| TspGWI | ACGGA | 1 cut(s) | 589 |
| VpaK11BI | GGWCC | 1 cut(s) | 169 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 93 |
| ZrmI | AGTACT | 1 cut(s) | 35 |