Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 97 - 359 | 3.3e-35 | Phosphofructokinase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 822 |
| Acc36I | ACCTGC | 1 cut(s) | 369 |
| AccB1I | GGYRCC | 3 cut(s) | 277, 319, 669 |
| AccI | GTMKAC | 1 cut(s) | 788 |
| AccIII | TCCGGA | 1 cut(s) | 49 |
| AciI | CCGC | 5 cut(s) | 36, 171, 180, 183, 1332 |
| AclWI | GGATC | 2 cut(s) | 52, 1627 |
| AcoI | YGGCCR | 1 cut(s) | 229 |
| AcsI | RAATTY | 3 cut(s) | 1059, 1345, 1574 |
| AcuI | CTGAAG | 4 cut(s) | 564, 619, 1145, 1167 |
| AdeI | CACNNNGTG | 2 cut(s) | 326, 1589 |
| AfaI | GTAC | 2 cut(s) | 800, 1392 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 68, 186 |
| AhlI | ACTAGT | 1 cut(s) | 716 |
| AjiI | CACGTC | 1 cut(s) | 74 |
| AjnI | CCWGG | 5 cut(s) | 322, 474, 1197, 1624, 1648 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 551, 583 |
| Alw21I | GWGCWC | 2 cut(s) | 1041, 1564 |
| Alw26I | GTCTC | 4 cut(s) | 501, 1173, 1208, 1501 |
| Alw44I | GTGCAC | 1 cut(s) | 1560 |
| AlwI | GGATC | 2 cut(s) | 52, 1627 |
| Ama87I | CYCGRG | 2 cut(s) | 657, 1688 |
| Aor13HI | TCCGGA | 1 cut(s) | 49 |
| AoxI | GGCC | 2 cut(s) | 3, 229 |
| ApaLI | GTGCAC | 1 cut(s) | 1560 |
| ApeKI | GCWGC | 2 cut(s) | 830, 1442 |
| ApoI | RAATTY | 3 cut(s) | 1059, 1345, 1574 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 456, 1152 |
| AspLEI | GCGC | 1 cut(s) | 1385 |
| AspS9I | GGNCC | 6 cut(s) | 4, 167, 407, 1030, 1628, 1646 |
| AsuC2I | CCSGG | 2 cut(s) | 187, 219 |
| AsuHPI | GGTGA | 4 cut(s) | 25, 701, 860, 1102 |
| AvaI | CYCGRG | 2 cut(s) | 657, 1688 |
| AvaII | GGWCC | 5 cut(s) | 167, 407, 1030, 1628, 1646 |
| AxyI | CCTNAGG | 1 cut(s) | 1026 |
| BaeGI | GKGCMC | 3 cut(s) | 333, 674, 1564 |
| BalI | TGGCCA | 1 cut(s) | 231 |
| BanI | GGYRCC | 3 cut(s) | 277, 319, 669 |
| BanII | GRGCYC | 2 cut(s) | 1041, 1696 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1598, 1630 |
| BauI | CACGAG | 1 cut(s) | 24 |
| BbsI | GAAGAC | 1 cut(s) | 1415 |
| Bbv12I | GWGCWC | 2 cut(s) | 1041, 1564 |
| BbvI | GCAGC | 2 cut(s) | 817, 1429 |
| BccI | CCATC | 4 cut(s) | 244, 566, 680, 1486 |
| BceAI | ACGGC | 3 cut(s) | 2, 23, 90 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 255, 289 |
| BciT130I | CCWGG | 5 cut(s) | 324, 476, 1199, 1626, 1650 |
| BclI | TGATCA | 1 cut(s) | 1530 |
| BcnI | CCSGG | 2 cut(s) | 187, 219 |
| BcoDI | GTCTC | 4 cut(s) | 501, 1173, 1208, 1501 |
| BcuI | ACTAGT | 1 cut(s) | 716 |
| BfaI | CTAG | 2 cut(s) | 174, 717 |
| BfmI | CTRYAG | 2 cut(s) | 496, 1464 |
| BfoI | RGCGCY | 1 cut(s) | 1386 |
| BfuAI | ACCTGC | 1 cut(s) | 369 |
| BglII | AGATCT | 1 cut(s) | 28 |
| BisI | GCNGC | 4 cut(s) | 183, 831, 1333, 1443 |
| BlsI | GCNGC | 4 cut(s) | 184, 832, 1334, 1444 |
| BmcAI | AGTACT | 2 cut(s) | 800, 1392 |
| Bme1390I | CCNGG | 7 cut(s) | 187, 219, 324, 476, 1199, 1626, 1650 |
| Bme18I | GGWCC | 5 cut(s) | 167, 407, 1030, 1628, 1646 |
| BmeT110I | CYCGRG | 2 cut(s) | 657, 1688 |
| BmgBI | CACGTC | 1 cut(s) | 74 |
| BmgT120I | GGNCC | 6 cut(s) | 4, 167, 407, 1030, 1628, 1646 |
| BmiI | GGNNCC | 7 cut(s) | 6, 169, 252, 279, 321, 671, 711 |
| BmrFI | CCNGG | 7 cut(s) | 187, 219, 324, 476, 1199, 1626, 1650 |
| BmsI | GCATC | 7 cut(s) | 253, 769, 1158, 1215, 1319, 1342, 1589 |
| BpiI | GAAGAC | 1 cut(s) | 1415 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1380, 1412 |
| BpmI | CTGGAG | 2 cut(s) | 330, 1407 |
| BpuEI | CTTGAG | 2 cut(s) | 869, 1193 |
| BpuMI | CCSGG | 2 cut(s) | 187, 219 |
| BsaI | GGTCTC | 1 cut(s) | 1173 |
| BsaJI | CCNNGG | 2 cut(s) | 475, 1624 |
| BsaWI | WCCGGW | 2 cut(s) | 49, 1412 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 882, 912, 1498, 1528 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 68, 186 |
| Bse1I | ACTGG | 3 cut(s) | 529, 796, 1648 |
| Bse21I | CCTNAGG | 1 cut(s) | 1026 |
| Bse3DI | GCAATG | 2 cut(s) | 1041, 1548 |
| BseAI | TCCGGA | 1 cut(s) | 49 |
| BseBI | CCWGG | 5 cut(s) | 324, 476, 1199, 1626, 1650 |
| BseDI | CCNNGG | 2 cut(s) | 475, 1624 |
| BseGI | GGATG | 4 cut(s) | 268, 577, 952, 1501 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 68, 186 |
| BseMI | GCAATG | 2 cut(s) | 1041, 1548 |
| BseMII | CTCAG | 3 cut(s) | 1017, 1209, 1678 |
| BseNI | ACTGG | 3 cut(s) | 529, 796, 1648 |
| BseRI | GAGGAG | 2 cut(s) | 1385, 1519 |
| BseSI | GKGCMC | 3 cut(s) | 333, 674, 1564 |
| BseXI | GCAGC | 2 cut(s) | 817, 1429 |
| BshFI | GGCC | 2 cut(s) | 5, 231 |
| BshNI | GGYRCC | 3 cut(s) | 277, 319, 669 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1041, 1564 |
| BsiHKCI | CYCGRG | 2 cut(s) | 657, 1688 |
| BsiSI | CCGG | 4 cut(s) | 50, 186, 218, 1413 |
| BslFI | GGGAC | 4 cut(s) | 153, 1299, 1311, 1486 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 68, 186 |
| BsmAI | GTCTC | 4 cut(s) | 501, 1173, 1208, 1501 |
| BsmFI | GGGAC | 4 cut(s) | 153, 1299, 1311, 1486 |
| BsmI | GAATGC | 2 cut(s) | 61, 451 |
| BsnI | GGCC | 2 cut(s) | 5, 231 |
| Bso31I | GGTCTC | 1 cut(s) | 1173 |
| BsoBI | CYCGRG | 2 cut(s) | 657, 1688 |
| Bsp1286I | GDGCHC | 5 cut(s) | 333, 674, 1041, 1564, 1696 |
| Bsp13I | TCCGGA | 1 cut(s) | 49 |
| Bsp143I | GATC | 9 cut(s) | 28, 44, 256, 492, 759, 1011, 1135, 1530, 1632 |
| BspACI | CCGC | 5 cut(s) | 36, 171, 180, 183, 1332 |
| BspANI | GGCC | 2 cut(s) | 5, 231 |
| BspCNI | CTCAG | 3 cut(s) | 1018, 1210, 1677 |
| BspEI | TCCGGA | 1 cut(s) | 49 |
| BspHI | TCATGA | 2 cut(s) | 420, 771 |
| BspLI | GGNNCC | 7 cut(s) | 6, 169, 252, 279, 321, 671, 711 |
| BspMAI | CTGCAG | 1 cut(s) | 500 |
| BspMI | ACCTGC | 1 cut(s) | 369 |
| BspPI | GGATC | 2 cut(s) | 52, 1627 |
| BspT107I | GGYRCC | 3 cut(s) | 277, 319, 669 |
| BspTNI | GGTCTC | 1 cut(s) | 1173 |
| BsrDI | GCAATG | 2 cut(s) | 1041, 1548 |
| BsrI | ACTGG | 3 cut(s) | 529, 796, 1648 |
| BssECI | CCNNGG | 2 cut(s) | 475, 1624 |
| BssMI | GATC | 9 cut(s) | 28, 44, 256, 492, 759, 1011, 1135, 1530, 1632 |
| BssSI | CACGAG | 1 cut(s) | 24 |
| Bst2BI | CACGAG | 1 cut(s) | 24 |
| Bst2UI | CCWGG | 5 cut(s) | 324, 476, 1199, 1626, 1650 |
| Bst4CI | ACNGT | 8 cut(s) | 89, 351, 986, 1302, 1364, 1403, 1465, 1514 |
| Bst6I | CTCTTC | 3 cut(s) | 126, 499, 888 |
| BstC8I | GCNNGC | 6 cut(s) | 233, 372, 616, 739, 1269, 1337 |
| BstDEI | CTNAG | 4 cut(s) | 1026, 1206, 1218, 1664 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 66 |
| BstF5I | GGATG | 4 cut(s) | 268, 577, 952, 1501 |
| BstH2I | RGCGCY | 1 cut(s) | 1386 |
| BstHHI | GCGC | 1 cut(s) | 1385 |
| BstKTI | GATC | 9 cut(s) | 31, 47, 259, 495, 762, 1014, 1138, 1533, 1635 |
| BstMAI | GTCTC | 4 cut(s) | 501, 1173, 1208, 1501 |
| BstMBI | GATC | 9 cut(s) | 28, 44, 256, 492, 759, 1011, 1135, 1530, 1632 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 179, 1114, 1484 |
| BstNI | CCWGG | 5 cut(s) | 324, 476, 1199, 1626, 1650 |
| BstSCI | CCNGG | 7 cut(s) | 185, 217, 322, 474, 1197, 1624, 1648 |
| BstSFI | CTRYAG | 2 cut(s) | 496, 1464 |
| BstSLI | GKGCMC | 3 cut(s) | 333, 674, 1564 |
| BstV1I | GCAGC | 2 cut(s) | 817, 1429 |
| BstV2I | GAAGAC | 1 cut(s) | 1415 |
| BstX2I | RGATCY | 2 cut(s) | 28, 44 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1643 |
| BstYI | RGATCY | 2 cut(s) | 28, 44 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1026 |
| BsuRI | GGCC | 2 cut(s) | 5, 231 |
| BtrI | CACGTC | 1 cut(s) | 74 |
| BtsCI | GGATG | 4 cut(s) | 268, 577, 952, 1501 |
| BtsI | GCAGTG | 3 cut(s) | 252, 505, 1583 |
| BtsIMutI | CAGTG | 6 cut(s) | 94, 252, 505, 1408, 1583, 1641 |
| BveI | ACCTGC | 1 cut(s) | 369 |
| Cac8I | GCNNGC | 6 cut(s) | 233, 372, 616, 739, 1269, 1337 |
| CciI | TCATGA | 2 cut(s) | 420, 771 |
| CfoI | GCGC | 1 cut(s) | 1385 |
| Cfr13I | GGNCC | 6 cut(s) | 4, 167, 407, 1030, 1628, 1646 |
| CsiI | ACCWGGT | 2 cut(s) | 322, 1648 |
| Csp6I | GTAC | 2 cut(s) | 799, 1391 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1330, 1365 |
| CviAII | CATG | 5 cut(s) | 421, 490, 772, 940, 1294 |
| CviQI | GTAC | 2 cut(s) | 799, 1391 |
| DdeI | CTNAG | 4 cut(s) | 1026, 1206, 1218, 1664 |
| DpnI | GATC | 9 cut(s) | 30, 46, 258, 494, 761, 1013, 1137, 1532, 1634 |
| DpnII | GATC | 9 cut(s) | 28, 44, 256, 492, 759, 1011, 1135, 1530, 1632 |
| DraI | TTTAAA | 1 cut(s) | 535 |
| DraIII | CACNNNGTG | 2 cut(s) | 326, 1589 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 822 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 822 |
| EaeI | YGGCCR | 1 cut(s) | 229 |
| Eam1104I | CTCTTC | 3 cut(s) | 126, 499, 888 |
| EarI | CTCTTC | 3 cut(s) | 126, 499, 888 |
| EciI | GGCGGA | 1 cut(s) | 169 |
| Ecl136II | GAGCTC | 1 cut(s) | 1039 |
| Eco24I | GRGCYC | 2 cut(s) | 1041, 1696 |
| Eco31I | GGTCTC | 1 cut(s) | 1173 |
| Eco47I | GGWCC | 5 cut(s) | 167, 407, 1030, 1628, 1646 |
| Eco53kI | GAGCTC | 1 cut(s) | 1039 |
| Eco57I | CTGAAG | 4 cut(s) | 564, 619, 1145, 1167 |
| Eco81I | CCTNAGG | 1 cut(s) | 1026 |
| Eco88I | CYCGRG | 2 cut(s) | 657, 1688 |
| EcoICRI | GAGCTC | 1 cut(s) | 1039 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 66 |
| EcoO109I | RGGNCCY | 1 cut(s) | 167 |
| EcoRII | CCWGG | 5 cut(s) | 322, 474, 1197, 1624, 1648 |
| EcoT38I | GRGCYC | 2 cut(s) | 1041, 1696 |
| FaeI | CATG | 5 cut(s) | 424, 493, 775, 943, 1297 |
| FaqI | GGGAC | 4 cut(s) | 153, 1299, 1311, 1486 |
| FatI | CATG | 5 cut(s) | 420, 489, 771, 939, 1293 |
| FauI | CCCGC | 1 cut(s) | 178 |
| FbaI | TGATCA | 1 cut(s) | 1530 |
| FblI | GTMKAC | 1 cut(s) | 788 |
| Fnu4HI | GCNGC | 4 cut(s) | 183, 831, 1333, 1443 |
| FokI | GGATG | 4 cut(s) | 275, 584, 959, 1508 |
| FriOI | GRGCYC | 2 cut(s) | 1041, 1696 |
| Fsp4HI | GCNGC | 4 cut(s) | 183, 831, 1333, 1443 |
| FspBI | CTAG | 2 cut(s) | 174, 717 |
| GlaI | GCGC | 1 cut(s) | 1384 |
| GluI | GCNGC | 4 cut(s) | 183, 831, 1333, 1443 |
| GsuI | CTGGAG | 2 cut(s) | 330, 1407 |
| HaeII | RGCGCY | 1 cut(s) | 1386 |
| HaeIII | GGCC | 2 cut(s) | 5, 231 |
| HapII | CCGG | 4 cut(s) | 50, 186, 218, 1413 |
| HhaI | GCGC | 1 cut(s) | 1385 |
| Hin1II | CATG | 5 cut(s) | 424, 493, 775, 943, 1297 |
| Hin6I | GCGC | 1 cut(s) | 1383 |
| HinP1I | GCGC | 1 cut(s) | 1383 |
| HincII | GTYRAC | 1 cut(s) | 789 |
| HindII | GTYRAC | 1 cut(s) | 789 |
| HindIII | AAGCTT | 1 cut(s) | 560 |
| HinfI | GANTC | 5 cut(s) | 190, 599, 660, 997, 1658 |
| HpaII | CCGG | 4 cut(s) | 50, 186, 218, 1413 |
| HphI | GGTGA | 4 cut(s) | 25, 701, 860, 1102 |
| Hpy166II | GTNNAC | 3 cut(s) | 85, 789, 1562 |
| Hpy188I | TCNGA | 6 cut(s) | 44, 754, 764, 1011, 1632, 1657 |
| Hpy8I | GTNNAC | 3 cut(s) | 85, 789, 1562 |
| Hpy99I | CGWCG | 3 cut(s) | 44, 164, 277 |
| HpyAV | CCTTC | 7 cut(s) | 141, 158, 279, 629, 998, 1316, 1550 |
| HpyCH4III | ACNGT | 8 cut(s) | 89, 351, 986, 1302, 1364, 1403, 1465, 1514 |
| HpyCH4IV | ACGT | 3 cut(s) | 73, 768, 961 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 179, 1114, 1484 |
| HpyF3I | CTNAG | 4 cut(s) | 1026, 1206, 1218, 1664 |
| HpySE526I | ACGT | 3 cut(s) | 73, 768, 961 |
| Hsp92II | CATG | 5 cut(s) | 424, 493, 775, 943, 1297 |
| HspAI | GCGC | 1 cut(s) | 1383 |
| Kpn2I | TCCGGA | 1 cut(s) | 49 |
| Ksp22I | TGATCA | 1 cut(s) | 1530 |
| Kzo9I | GATC | 9 cut(s) | 28, 44, 256, 492, 759, 1011, 1135, 1530, 1632 |
| LmnI | GCTCC | 3 cut(s) | 64, 146, 1450 |
| Lsp1109I | GCAGC | 2 cut(s) | 817, 1429 |
| LweI | GCATC | 7 cut(s) | 253, 769, 1158, 1215, 1319, 1342, 1589 |
| MabI | ACCWGGT | 2 cut(s) | 322, 1648 |
| MaeI | CTAG | 2 cut(s) | 174, 717 |
| MaeII | ACGT | 3 cut(s) | 73, 768, 961 |
| MaeIII | GTNAC | 4 cut(s) | 19, 928, 974, 1302 |
| MalI | GATC | 9 cut(s) | 30, 46, 258, 494, 761, 1013, 1137, 1532, 1634 |
| MboI | GATC | 9 cut(s) | 28, 44, 256, 492, 759, 1011, 1135, 1530, 1632 |
| MflI | RGATCY | 2 cut(s) | 28, 44 |
| MhlI | GDGCHC | 5 cut(s) | 333, 674, 1041, 1564, 1696 |
| MlsI | TGGCCA | 1 cut(s) | 231 |
| MluCI | AATT | 9 cut(s) | 284, 1059, 1152, 1250, 1289, 1345, 1419, 1574, 1637 |
| MluNI | TGGCCA | 1 cut(s) | 231 |
| MlyI | GAGTC | 2 cut(s) | 669, 1652 |
| MmeI | TCCRAC | 2 cut(s) | 22, 627 |
| MnlI | CCTC | 7 cut(s) | 305, 700, 889, 1021, 1066, 1406, 1497 |
| Mox20I | TGGCCA | 1 cut(s) | 231 |
| MroI | TCCGGA | 1 cut(s) | 49 |
| MroXI | GAANNNNTTC | 2 cut(s) | 456, 1152 |
| MscI | TGGCCA | 1 cut(s) | 231 |
| MseI | TTAA | 3 cut(s) | 111, 534, 1569 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1191 |
| Msp20I | TGGCCA | 1 cut(s) | 231 |
| MspA1I | CMGCKG | 2 cut(s) | 413, 1165 |
| MspI | CCGG | 4 cut(s) | 50, 186, 218, 1413 |
| MspR9I | CCNGG | 7 cut(s) | 187, 219, 324, 476, 1199, 1626, 1650 |
| MssI | GTTTAAAC | 1 cut(s) | 535 |
| Mva1269I | GAATGC | 2 cut(s) | 61, 451 |
| MvaI | CCWGG | 5 cut(s) | 324, 476, 1199, 1626, 1650 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 179, 1114, 1484 |
| NciI | CCSGG | 2 cut(s) | 187, 219 |
| NdeII | GATC | 9 cut(s) | 28, 44, 256, 492, 759, 1011, 1135, 1530, 1632 |
| NlaIII | CATG | 5 cut(s) | 424, 493, 775, 943, 1297 |
| NlaIV | GGNNCC | 7 cut(s) | 6, 169, 252, 279, 321, 671, 711 |
| NmuCI | GTSAC | 2 cut(s) | 928, 1302 |
| PagI | TCATGA | 2 cut(s) | 420, 771 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 278 |
| PctI | GAATGC | 2 cut(s) | 61, 451 |
| PdmI | GAANNNNTTC | 2 cut(s) | 456, 1152 |
| PfeI | GAWTC | 3 cut(s) | 190, 599, 997 |
| PkrI | GCNGC | 4 cut(s) | 184, 832, 1334, 1444 |
| PleI | GAGTC | 2 cut(s) | 668, 1652 |
| PmeI | GTTTAAAC | 1 cut(s) | 535 |
| PpsI | GAGTC | 2 cut(s) | 668, 1652 |
| PpuMI | RGGWCCY | 1 cut(s) | 167 |
| Psp124BI | GAGCTC | 1 cut(s) | 1041 |
| Psp5II | RGGWCCY | 1 cut(s) | 167 |
| Psp6I | CCWGG | 5 cut(s) | 322, 474, 1197, 1624, 1648 |
| PspGI | CCWGG | 5 cut(s) | 322, 474, 1197, 1624, 1648 |
| PspN4I | GGNNCC | 7 cut(s) | 6, 169, 252, 279, 321, 671, 711 |
| PspPI | GGNCC | 6 cut(s) | 4, 167, 407, 1030, 1628, 1646 |
| PspPPI | RGGWCCY | 1 cut(s) | 167 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 234, 266 |
| PstI | CTGCAG | 1 cut(s) | 500 |
| PsuI | RGATCY | 2 cut(s) | 28, 44 |
| PvuII | CAGCTG | 2 cut(s) | 413, 1165 |
| RsaI | GTAC | 2 cut(s) | 800, 1392 |
| RsaNI | GTAC | 2 cut(s) | 799, 1391 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1191 |
| SacI | GAGCTC | 1 cut(s) | 1041 |
| SalI | GTCGAC | 1 cut(s) | 787 |
| SaqAI | TTAA | 3 cut(s) | 111, 534, 1569 |
| SatI | GCNGC | 4 cut(s) | 183, 831, 1333, 1443 |
| Sau3AI | GATC | 9 cut(s) | 28, 44, 256, 492, 759, 1011, 1135, 1530, 1632 |
| Sau96I | GGNCC | 6 cut(s) | 4, 167, 407, 1030, 1628, 1646 |
| ScaI | AGTACT | 2 cut(s) | 800, 1392 |
| SchI | GAGTC | 2 cut(s) | 669, 1652 |
| ScrFI | CCNGG | 7 cut(s) | 187, 219, 324, 476, 1199, 1626, 1650 |
| SduI | GDGCHC | 5 cut(s) | 333, 674, 1041, 1564, 1696 |
| SexAI | ACCWGGT | 2 cut(s) | 322, 1648 |
| SfaNI | GCATC | 7 cut(s) | 253, 769, 1158, 1215, 1319, 1342, 1589 |
| SfcI | CTRYAG | 2 cut(s) | 496, 1464 |
| SinI | GGWCC | 5 cut(s) | 167, 407, 1030, 1628, 1646 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1191 |
| SmlI | CTYRAG | 2 cut(s) | 848, 1172 |
| SmoI | CTYRAG | 2 cut(s) | 848, 1172 |
| SpeI | ACTAGT | 1 cut(s) | 716 |
| Sse9I | AATT | 9 cut(s) | 284, 1059, 1152, 1250, 1289, 1345, 1419, 1574, 1637 |
| SsiI | CCGC | 5 cut(s) | 36, 171, 180, 183, 1332 |
| SspMI | CTAG | 2 cut(s) | 174, 717 |
| SstI | GAGCTC | 1 cut(s) | 1041 |
| StyD4I | CCNGG | 7 cut(s) | 185, 217, 322, 474, 1197, 1624, 1648 |
| TaaI | ACNGT | 8 cut(s) | 89, 351, 986, 1302, 1364, 1403, 1465, 1514 |
| TaiI | ACGT | 3 cut(s) | 76, 771, 964 |
| TaqI | TCGA | 5 cut(s) | 162, 788, 1014, 1080, 1500 |
| TasI | AATT | 9 cut(s) | 284, 1059, 1152, 1250, 1289, 1345, 1419, 1574, 1637 |
| TatI | WGTACW | 2 cut(s) | 798, 1390 |
| TauI | GCSGC | 2 cut(s) | 185, 1335 |
| TfiI | GAWTC | 3 cut(s) | 190, 599, 997 |
| Tru1I | TTAA | 3 cut(s) | 111, 534, 1569 |
| Tru9I | TTAA | 3 cut(s) | 111, 534, 1569 |
| TscAI | CASTG | 6 cut(s) | 94, 252, 505, 1408, 1590, 1648 |
| TseFI | GTSAC | 2 cut(s) | 928, 1302 |
| TseI | GCWGC | 2 cut(s) | 830, 1442 |
| Tsp45I | GTSAC | 2 cut(s) | 928, 1302 |
| TspDTI | ATGAA | 5 cut(s) | 437, 1071, 1098, 1282, 1335 |
| TspGWI | ACGGA | 1 cut(s) | 1203 |
| TspRI | CASTG | 6 cut(s) | 94, 252, 505, 1408, 1590, 1648 |
| VneI | GTGCAC | 1 cut(s) | 1560 |
| VpaK11BI | GGWCC | 5 cut(s) | 167, 407, 1030, 1628, 1646 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 66 |
| XapI | RAATTY | 3 cut(s) | 1059, 1345, 1574 |
| XmiI | GTMKAC | 1 cut(s) | 788 |
| XmnI | GAANNNNTTC | 2 cut(s) | 456, 1152 |
| XspI | CTAG | 2 cut(s) | 174, 717 |
| ZrmI | AGTACT | 2 cut(s) | 800, 1392 |