Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 99 - 304 | 1.2e-34 | Phosphofructokinase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 3 cut(s) | 87, 165, 277 |
| AccII | CGCG | 2 cut(s) | 209, 378 |
| AciI | CCGC | 7 cut(s) | 30, 174, 183, 186, 376, 411, 836 |
| AcoI | YGGCCR | 1 cut(s) | 70 |
| AcsI | RAATTY | 1 cut(s) | 354 |
| AcuI | CTGAAG | 2 cut(s) | 625, 893 |
| AdeI | CACNNNGTG | 1 cut(s) | 343 |
| AfaI | GTAC | 3 cut(s) | 123, 819, 906 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 68 |
| AgsI | TTSAA | 7 cut(s) | 157, 295, 524, 649, 658, 703, 933 |
| AjnI | CCWGG | 1 cut(s) | 99 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 557, 589 |
| AluBI | AGCT | 4 cut(s) | 180, 419, 560, 568 |
| AluI | AGCT | 4 cut(s) | 180, 419, 560, 568 |
| Alw21I | GWGCWC | 1 cut(s) | 144 |
| Alw26I | GTCTC | 1 cut(s) | 85 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 62 |
| Ama87I | CYCGRG | 1 cut(s) | 455 |
| AoxI | GGCC | 4 cut(s) | 70, 102, 319, 413 |
| ApeKI | GCWGC | 1 cut(s) | 35 |
| ApoI | RAATTY | 1 cut(s) | 354 |
| AspLEI | GCGC | 1 cut(s) | 380 |
| AspS9I | GGNCC | 4 cut(s) | 97, 170, 413, 715 |
| AsuC2I | CCSGG | 2 cut(s) | 330, 790 |
| AsuHPI | GGTGA | 2 cut(s) | 58, 707 |
| AsuNHI | GCTAGC | 1 cut(s) | 176 |
| AvaI | CYCGRG | 1 cut(s) | 455 |
| AvaII | GGWCC | 3 cut(s) | 97, 170, 715 |
| AxyI | CCTNAGG | 1 cut(s) | 198 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 896, 929 |
| BauI | CACGAG | 1 cut(s) | 663 |
| Bbv12I | GWGCWC | 1 cut(s) | 144 |
| BbvI | GCAGC | 1 cut(s) | 22 |
| BccI | CCATC | 3 cut(s) | 19, 247, 572 |
| BceAI | ACGGC | 1 cut(s) | 43 |
| BciT130I | CCWGG | 1 cut(s) | 101 |
| BcnI | CCSGG | 2 cut(s) | 330, 790 |
| BcoDI | GTCTC | 1 cut(s) | 85 |
| BfaI | CTAG | 1 cut(s) | 177 |
| BfmI | CTRYAG | 1 cut(s) | 88 |
| BisI | GCNGC | 3 cut(s) | 36, 186, 837 |
| BlsI | GCNGC | 3 cut(s) | 37, 187, 838 |
| Bme1390I | CCNGG | 3 cut(s) | 101, 330, 790 |
| Bme18I | GGWCC | 3 cut(s) | 97, 170, 715 |
| BmeT110I | CYCGRG | 1 cut(s) | 455 |
| BmgT120I | GGNCC | 4 cut(s) | 97, 170, 413, 715 |
| BmiI | GGNNCC | 5 cut(s) | 6, 172, 255, 716, 717 |
| BmrFI | CCNGG | 3 cut(s) | 101, 330, 790 |
| BmsI | GCATC | 1 cut(s) | 775 |
| BmtI | GCTAGC | 1 cut(s) | 180 |
| BpmI | CTGGAG | 2 cut(s) | 336, 513 |
| BpuMI | CCSGG | 2 cut(s) | 330, 790 |
| BsaJI | CCNNGG | 1 cut(s) | 328 |
| BsaWI | WCCGGW | 1 cut(s) | 280 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 114, 144, 275, 305, 888, 918 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 68 |
| Bse118I | RCCGGY | 3 cut(s) | 38, 68, 188 |
| Bse1I | ACTGG | 2 cut(s) | 105, 802 |
| Bse21I | CCTNAGG | 1 cut(s) | 198 |
| Bse3DI | GCAATG | 2 cut(s) | 778, 943 |
| BseBI | CCWGG | 1 cut(s) | 101 |
| BseDI | CCNNGG | 1 cut(s) | 328 |
| BseGI | GGATG | 2 cut(s) | 583, 874 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 68 |
| BseMI | GCAATG | 2 cut(s) | 778, 943 |
| BseMII | CTCAG | 2 cut(s) | 36, 189 |
| BseNI | ACTGG | 2 cut(s) | 105, 802 |
| BseRI | GAGGAG | 1 cut(s) | 215 |
| BseX3I | CGGCCG | 1 cut(s) | 70 |
| BseXI | GCAGC | 1 cut(s) | 22 |
| BseYI | CCCAGC | 1 cut(s) | 419 |
| BsgI | GTGCAG | 1 cut(s) | 228 |
| Bsh1236I | CGCG | 2 cut(s) | 209, 378 |
| Bsh1285I | CGRYCG | 1 cut(s) | 73 |
| BshFI | GGCC | 4 cut(s) | 72, 104, 321, 415 |
| BsiEI | CGRYCG | 1 cut(s) | 73 |
| BsiHKAI | GWGCWC | 1 cut(s) | 144 |
| BsiHKCI | CYCGRG | 1 cut(s) | 455 |
| BsiSI | CCGG | 6 cut(s) | 39, 69, 189, 281, 330, 790 |
| BslFI | GGGAC | 3 cut(s) | 156, 527, 701 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 68 |
| BsmAI | GTCTC | 1 cut(s) | 85 |
| BsmBI | CGTCTC | 1 cut(s) | 85 |
| BsmFI | GGGAC | 3 cut(s) | 156, 527, 701 |
| BsnI | GGCC | 4 cut(s) | 72, 104, 321, 415 |
| BsoBI | CYCGRG | 1 cut(s) | 455 |
| Bsp1286I | GDGCHC | 1 cut(s) | 144 |
| Bsp143I | GATC | 2 cut(s) | 204, 297 |
| Bsp68I | TCGCGA | 1 cut(s) | 209 |
| BspACI | CCGC | 7 cut(s) | 30, 174, 183, 186, 376, 411, 836 |
| BspANI | GGCC | 4 cut(s) | 72, 104, 321, 415 |
| BspCNI | CTCAG | 2 cut(s) | 37, 190 |
| BspFNI | CGCG | 2 cut(s) | 209, 378 |
| BspLI | GGNNCC | 5 cut(s) | 6, 172, 255, 716, 717 |
| BspOI | GCTAGC | 1 cut(s) | 180 |
| BsrDI | GCAATG | 2 cut(s) | 778, 943 |
| BsrFI | RCCGGY | 3 cut(s) | 38, 68, 188 |
| BsrI | ACTGG | 2 cut(s) | 105, 802 |
| BssAI | RCCGGY | 3 cut(s) | 38, 68, 188 |
| BssECI | CCNNGG | 1 cut(s) | 328 |
| BssMI | GATC | 2 cut(s) | 204, 297 |
| BssSI | CACGAG | 1 cut(s) | 663 |
| Bst2BI | CACGAG | 1 cut(s) | 663 |
| Bst2UI | CCWGG | 1 cut(s) | 101 |
| Bst4CI | ACNGT | 3 cut(s) | 170, 249, 695 |
| Bst6I | CTCTTC | 1 cut(s) | 894 |
| BstC8I | GCNNGC | 7 cut(s) | 178, 190, 413, 417, 622, 745, 834 |
| BstDEI | CTNAG | 2 cut(s) | 45, 198 |
| BstF5I | GGATG | 2 cut(s) | 583, 874 |
| BstFNI | CGCG | 2 cut(s) | 209, 378 |
| BstHHI | GCGC | 1 cut(s) | 380 |
| BstKTI | GATC | 2 cut(s) | 207, 300 |
| BstMAI | GTCTC | 1 cut(s) | 85 |
| BstMBI | GATC | 2 cut(s) | 204, 297 |
| BstMCI | CGRYCG | 1 cut(s) | 73 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 41, 148, 182, 945 |
| BstNI | CCWGG | 1 cut(s) | 101 |
| BstSCI | CCNGG | 3 cut(s) | 99, 328, 788 |
| BstSFI | CTRYAG | 1 cut(s) | 88 |
| BstUI | CGCG | 2 cut(s) | 209, 378 |
| BstV1I | GCAGC | 1 cut(s) | 22 |
| BstZI | CGGCCG | 1 cut(s) | 70 |
| Bsu36I | CCTNAGG | 1 cut(s) | 198 |
| BsuRI | GGCC | 4 cut(s) | 72, 104, 321, 415 |
| BtsCI | GGATG | 2 cut(s) | 583, 874 |
| BtsI | GCAGTG | 2 cut(s) | 511, 738 |
| BtsIMutI | CAGTG | 4 cut(s) | 14, 245, 511, 738 |
| BtuMI | TCGCGA | 1 cut(s) | 209 |
| Cac8I | GCNNGC | 7 cut(s) | 178, 190, 413, 417, 622, 745, 834 |
| CaiI | CAGNNNCTG | 1 cut(s) | 62 |
| CfoI | GCGC | 1 cut(s) | 380 |
| Cfr10I | RCCGGY | 3 cut(s) | 38, 68, 188 |
| Cfr13I | GGNCC | 4 cut(s) | 97, 170, 413, 715 |
| Csp6I | GTAC | 3 cut(s) | 122, 818, 905 |
| CviAII | CATG | 1 cut(s) | 119 |
| CviQI | GTAC | 3 cut(s) | 122, 818, 905 |
| DdeI | CTNAG | 2 cut(s) | 45, 198 |
| DpnI | GATC | 2 cut(s) | 206, 299 |
| DpnII | GATC | 2 cut(s) | 204, 297 |
| DraI | TTTAAA | 1 cut(s) | 541 |
| DraIII | CACNNNGTG | 1 cut(s) | 343 |
| EaeI | YGGCCR | 1 cut(s) | 70 |
| EagI | CGGCCG | 1 cut(s) | 70 |
| Eam1104I | CTCTTC | 1 cut(s) | 894 |
| EarI | CTCTTC | 1 cut(s) | 894 |
| EciI | GGCGGA | 1 cut(s) | 172 |
| EclXI | CGGCCG | 1 cut(s) | 70 |
| Eco32I | GATATC | 1 cut(s) | 348 |
| Eco47I | GGWCC | 3 cut(s) | 97, 170, 715 |
| Eco52I | CGGCCG | 1 cut(s) | 70 |
| Eco57I | CTGAAG | 2 cut(s) | 625, 893 |
| Eco81I | CCTNAGG | 1 cut(s) | 198 |
| Eco88I | CYCGRG | 1 cut(s) | 455 |
| EcoO109I | RGGNCCY | 1 cut(s) | 715 |
| EcoRII | CCWGG | 1 cut(s) | 99 |
| EcoRV | GATATC | 1 cut(s) | 348 |
| Esp3I | CGTCTC | 1 cut(s) | 85 |
| FaeI | CATG | 1 cut(s) | 122 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 891, 923 |
| FaqI | GGGAC | 3 cut(s) | 156, 527, 701 |
| FatI | CATG | 1 cut(s) | 118 |
| FauI | CCCGC | 2 cut(s) | 181, 404 |
| FblI | GTMKAC | 3 cut(s) | 87, 165, 277 |
| Fnu4HI | GCNGC | 3 cut(s) | 36, 186, 837 |
| FokI | GGATG | 2 cut(s) | 590, 861 |
| Fsp4HI | GCNGC | 3 cut(s) | 36, 186, 837 |
| FspBI | CTAG | 1 cut(s) | 177 |
| GlaI | GCGC | 1 cut(s) | 379 |
| GluI | GCNGC | 3 cut(s) | 36, 186, 837 |
| GsaI | CCCAGC | 1 cut(s) | 423 |
| GsuI | CTGGAG | 2 cut(s) | 336, 513 |
| HaeIII | GGCC | 4 cut(s) | 72, 104, 321, 415 |
| HapII | CCGG | 6 cut(s) | 39, 69, 189, 281, 330, 790 |
| HhaI | GCGC | 1 cut(s) | 380 |
| Hin1II | CATG | 1 cut(s) | 122 |
| Hin6I | GCGC | 1 cut(s) | 378 |
| HinP1I | GCGC | 1 cut(s) | 378 |
| HincII | GTYRAC | 2 cut(s) | 166, 795 |
| HindII | GTYRAC | 2 cut(s) | 166, 795 |
| HindIII | AAGCTT | 1 cut(s) | 566 |
| HinfI | GANTC | 6 cut(s) | 75, 263, 269, 499, 524, 605 |
| HpaII | CCGG | 6 cut(s) | 39, 69, 189, 281, 330, 790 |
| HphI | GGTGA | 2 cut(s) | 58, 707 |
| Hpy166II | GTNNAC | 4 cut(s) | 88, 166, 278, 795 |
| Hpy188I | TCNGA | 1 cut(s) | 268 |
| Hpy188III | TCNNGA | 5 cut(s) | 197, 208, 315, 351, 530 |
| Hpy8I | GTNNAC | 4 cut(s) | 88, 166, 278, 795 |
| Hpy99I | CGWCG | 1 cut(s) | 170 |
| HpyAV | CCTTC | 3 cut(s) | 144, 163, 504 |
| HpyCH4III | ACNGT | 3 cut(s) | 170, 249, 695 |
| HpyCH4V | TGCA | 6 cut(s) | 245, 370, 708, 747, 758, 868 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 41, 148, 182, 945 |
| HpyF3I | CTNAG | 2 cut(s) | 45, 198 |
| Hsp92II | CATG | 1 cut(s) | 122 |
| HspAI | GCGC | 1 cut(s) | 378 |
| KflI | GGGWCCC | 1 cut(s) | 715 |
| KroI | GCCGGC | 1 cut(s) | 188 |
| KroNI | GCCGGC | 1 cut(s) | 190 |
| Kzo9I | GATC | 2 cut(s) | 204, 297 |
| LmnI | GCTCC | 4 cut(s) | 10, 185, 253, 283 |
| Lsp1109I | GCAGC | 1 cut(s) | 22 |
| LweI | GCATC | 1 cut(s) | 775 |
| MaeI | CTAG | 1 cut(s) | 177 |
| MaeIII | GTNAC | 2 cut(s) | 64, 695 |
| MalI | GATC | 2 cut(s) | 206, 299 |
| MboI | GATC | 2 cut(s) | 204, 297 |
| MboII | GAAGA | 3 cut(s) | 307, 661, 911 |
| MhlI | GDGCHC | 1 cut(s) | 144 |
| MluCI | AATT | 3 cut(s) | 354, 888, 933 |
| MlyI | GAGTC | 1 cut(s) | 599 |
| MmeI | TCCRAC | 1 cut(s) | 231 |
| MnlI | CCTC | 7 cut(s) | 88, 138, 193, 311, 326, 635, 895 |
| MroNI | GCCGGC | 1 cut(s) | 188 |
| MseI | TTAA | 2 cut(s) | 540, 958 |
| MslI | CAYNNNNRTG | 1 cut(s) | 856 |
| MspA1I | CMGCKG | 2 cut(s) | 419, 560 |
| MspI | CCGG | 6 cut(s) | 39, 69, 189, 281, 330, 790 |
| MspR9I | CCNGG | 3 cut(s) | 101, 330, 790 |
| MssI | GTTTAAAC | 1 cut(s) | 541 |
| MvaI | CCWGG | 1 cut(s) | 101 |
| MvnI | CGCG | 2 cut(s) | 209, 378 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 41, 148, 182, 945 |
| NaeI | GCCGGC | 1 cut(s) | 190 |
| NciI | CCSGG | 2 cut(s) | 330, 790 |
| NdeII | GATC | 2 cut(s) | 204, 297 |
| NgoMIV | GCCGGC | 1 cut(s) | 188 |
| NheI | GCTAGC | 1 cut(s) | 176 |
| NlaIII | CATG | 1 cut(s) | 122 |
| NlaIV | GGNNCC | 5 cut(s) | 6, 172, 255, 716, 717 |
| NmuCI | GTSAC | 2 cut(s) | 64, 695 |
| NruI | TCGCGA | 1 cut(s) | 209 |
| PdiI | GCCGGC | 1 cut(s) | 190 |
| PfeI | GAWTC | 5 cut(s) | 75, 263, 269, 499, 524 |
| PkrI | GCNGC | 3 cut(s) | 37, 187, 838 |
| PleI | GAGTC | 1 cut(s) | 599 |
| PmeI | GTTTAAAC | 1 cut(s) | 541 |
| PpsI | GAGTC | 1 cut(s) | 599 |
| PpuMI | RGGWCCY | 1 cut(s) | 715 |
| Psp5II | RGGWCCY | 1 cut(s) | 715 |
| Psp6I | CCWGG | 1 cut(s) | 99 |
| PspFI | CCCAGC | 1 cut(s) | 419 |
| PspGI | CCWGG | 1 cut(s) | 99 |
| PspN4I | GGNNCC | 5 cut(s) | 6, 172, 255, 716, 717 |
| PspPI | GGNCC | 4 cut(s) | 97, 170, 413, 715 |
| PspPPI | RGGWCCY | 1 cut(s) | 715 |
| PstNI | CAGNNNCTG | 1 cut(s) | 62 |
| PvuII | CAGCTG | 2 cut(s) | 419, 560 |
| RruI | TCGCGA | 1 cut(s) | 209 |
| RsaI | GTAC | 3 cut(s) | 123, 819, 906 |
| RsaNI | GTAC | 3 cut(s) | 122, 818, 905 |
| RseI | CAYNNNNRTG | 1 cut(s) | 856 |
| SalI | GTCGAC | 1 cut(s) | 164 |
| SaqAI | TTAA | 2 cut(s) | 540, 958 |
| SatI | GCNGC | 3 cut(s) | 36, 186, 837 |
| Sau3AI | GATC | 2 cut(s) | 204, 297 |
| Sau96I | GGNCC | 4 cut(s) | 97, 170, 413, 715 |
| SchI | GAGTC | 1 cut(s) | 599 |
| ScrFI | CCNGG | 3 cut(s) | 101, 330, 790 |
| SduI | GDGCHC | 1 cut(s) | 144 |
| SfaNI | GCATC | 1 cut(s) | 775 |
| SfcI | CTRYAG | 1 cut(s) | 88 |
| SinI | GGWCC | 3 cut(s) | 97, 170, 715 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 856 |
| Sse9I | AATT | 3 cut(s) | 354, 888, 933 |
| SsiI | CCGC | 7 cut(s) | 30, 174, 183, 186, 376, 411, 836 |
| SspI | AATATT | 1 cut(s) | 806 |
| SspMI | CTAG | 1 cut(s) | 177 |
| StyD4I | CCNGG | 3 cut(s) | 99, 328, 788 |
| TaaI | ACNGT | 3 cut(s) | 170, 249, 695 |
| TaqI | TCGA | 5 cut(s) | 114, 165, 360, 444, 492 |
| TaqII | GACCGA | 1 cut(s) | 221 |
| TasI | AATT | 3 cut(s) | 354, 888, 933 |
| TatI | WGTACW | 1 cut(s) | 121 |
| TauI | GCSGC | 2 cut(s) | 188, 839 |
| TfiI | GAWTC | 5 cut(s) | 75, 263, 269, 499, 524 |
| Tru1I | TTAA | 2 cut(s) | 540, 958 |
| Tru9I | TTAA | 2 cut(s) | 540, 958 |
| TscAI | CASTG | 4 cut(s) | 21, 252, 511, 745 |
| TseFI | GTSAC | 2 cut(s) | 64, 695 |
| TseI | GCWGC | 1 cut(s) | 35 |
| Tsp45I | GTSAC | 2 cut(s) | 64, 695 |
| TspDTI | ATGAA | 2 cut(s) | 443, 912 |
| TspGWI | ACGGA | 2 cut(s) | 73, 127 |
| TspRI | CASTG | 4 cut(s) | 21, 252, 511, 745 |
| VpaK11BI | GGWCC | 3 cut(s) | 97, 170, 715 |
| XapI | RAATTY | 1 cut(s) | 354 |
| XmiI | GTMKAC | 3 cut(s) | 87, 165, 277 |
| XspI | CTAG | 1 cut(s) | 177 |