Rroxscaffold_4G00281720
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001357
Physical Location & Seq
Forward (+)
3914011 .. 3916419
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281720.1

Sequence Viewer

Length: 573 bp
ATGTATATGAATTATGACCCAACAAGTAGTGCCAATGATAGTGATACTAGAGCTGAAGTAGGTGTGAATGATGAACCTGATAGTGAGAATAGTGAGAATACTGGAGGGCATAATGCAGATACTAGTGCAGATACTAGCAAGAATAGTGTTGATAATGGAAATGCTAGTGTCAACAATGGTGGAATAGATGGTGGAATAGTTGCATCCAGTTTGCATGATAATTTGTTGCTTCATGACATCACTGCTGCTAATCTTGATCTTGATCATGAACTTCCTCATGAGCTTCAGTTCATTAATGACACTGCTCAAGACTACAATAATCACTCCATGCTAACAAGAGCAAAGAATGGAATTTTCAAGCAGAAAACTTTTCAAGACTTTTGTTCTTATTCTTGTGTTGTTCATCATAAACTCCTTGATGAATATGCTTATTTTTATGGATTTACTTCTATAATGGATGTGTGTGATCCTGTTGAACCTAAGAGCTTCAAAAGTGTAGTTAGTGTTTCGGAATGGGAACTTGCAATGCTTGAAGAAATTAATGCTCTTCGAAACAAGGTACTTGGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

190

Amino Acids

20.94

Weight (kDa)

4.41

Isoelectric Point (pI)

30.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 461
AcsI RAATTY 1 cut(s) 351
AcuI CTGAAG 2 cut(s) 75, 269
AfaI GTAC 1 cut(s) 561
AgsI TTSAA 5 cut(s) 358, 374, 476, 490, 533
AhlI ACTAGT 1 cut(s) 122
AluBI AGCT 3 cut(s) 53, 283, 486
AluI AGCT 3 cut(s) 53, 283, 486
AlwI GGATC 1 cut(s) 461
ApeKI GCWGC 1 cut(s) 245
ApoI RAATTY 1 cut(s) 351
AseI ATTAAT 2 cut(s) 294, 540
AsuII TTCGAA 1 cut(s) 550
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 182
BclI TGATCA 1 cut(s) 262
BcuI ACTAGT 1 cut(s) 122
BfaI CTAG 4 cut(s) 48, 123, 135, 165
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
BmsI GCATC 1 cut(s) 212
BpmI CTGGAG 1 cut(s) 123
Bpu14I TTCGAA 1 cut(s) 550
BpuEI CTTGAG 1 cut(s) 291
BsaBI GATNNNNATC 1 cut(s) 261
Bse1I ACTGG 2 cut(s) 106, 207
Bse3DI GCAATG 1 cut(s) 531
Bse8I GATNNNNATC 1 cut(s) 261
BseGI GGATG 2 cut(s) 203, 463
BseJI GATNNNNATC 1 cut(s) 261
BseMI GCAATG 1 cut(s) 531
BseNI ACTGG 2 cut(s) 106, 207
BseXI GCAGC 1 cut(s) 232
BsgI GTGCAG 1 cut(s) 147
Bsp119I TTCGAA 1 cut(s) 550
Bsp143I GATC 3 cut(s) 256, 262, 466
BspHI TCATGA 3 cut(s) 232, 265, 277
BspPI GGATC 1 cut(s) 461
BspQI GCTCTTC 1 cut(s) 552
BspT104I TTCGAA 1 cut(s) 550
BsrDI GCAATG 1 cut(s) 531
BsrI ACTGG 2 cut(s) 106, 207
BssMI GATC 3 cut(s) 256, 262, 466
Bst6I CTCTTC 1 cut(s) 552
BstBI TTCGAA 1 cut(s) 550
BstDEI CTNAG 1 cut(s) 480
BstF5I GGATG 2 cut(s) 203, 463
BstKTI GATC 3 cut(s) 259, 265, 469
BstMBI GATC 3 cut(s) 256, 262, 466
BstV1I GCAGC 1 cut(s) 232
BtsCI GGATG 2 cut(s) 203, 463
BtsI GCAGTG 2 cut(s) 240, 300
BtsIMutI CAGTG 2 cut(s) 240, 300
CciI TCATGA 3 cut(s) 232, 265, 277
Csp6I GTAC 1 cut(s) 560
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 5 cut(s) 215, 233, 266, 278, 328
CviJI RGCY 3 cut(s) 53, 283, 486
CviKI_1 RGCY 3 cut(s) 53, 283, 486
CviQI GTAC 1 cut(s) 560
DdeI CTNAG 1 cut(s) 480
DpnI GATC 3 cut(s) 258, 264, 468
DpnII GATC 3 cut(s) 256, 262, 466
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
Eco57I CTGAAG 2 cut(s) 75, 269
FaeI CATG 5 cut(s) 218, 236, 269, 281, 331
FatI CATG 5 cut(s) 214, 232, 265, 277, 327
FbaI TGATCA 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 246
FokI GGATG 2 cut(s) 190, 470
Fsp4HI GCNGC 1 cut(s) 246
FspBI CTAG 4 cut(s) 48, 123, 135, 165
GluI GCNGC 1 cut(s) 246
GsuI CTGGAG 1 cut(s) 123
Hin1II CATG 5 cut(s) 218, 236, 269, 281, 331
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 1 cut(s) 511
Hpy188III TCNNGA 7 cut(s) 233, 254, 260, 266, 278, 308, 374
Hpy8I GTNNAC 1 cut(s) 172
HpyCH4V TGCA 5 cut(s) 116, 128, 203, 214, 524
HpyF3I CTNAG 1 cut(s) 480
Hsp92II CATG 5 cut(s) 218, 236, 269, 281, 331
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 3 cut(s) 256, 262, 466
LguI GCTCTTC 1 cut(s) 552
LpnPI CCDG 4 cut(s) 87, 90, 220, 483
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 1 cut(s) 212
MaeI CTAG 4 cut(s) 48, 123, 135, 165
MalI GATC 3 cut(s) 258, 264, 468
MboI GATC 3 cut(s) 256, 262, 466
MboII GAAGA 2 cut(s) 539, 545
MluCI AATT 4 cut(s) 10, 220, 351, 537
MnlI CCTC 2 cut(s) 98, 285
MseI TTAA 2 cut(s) 294, 540
NdeII GATC 3 cut(s) 256, 262, 466
NlaIII CATG 5 cut(s) 218, 236, 269, 281, 331
NspV TTCGAA 1 cut(s) 550
PagI TCATGA 3 cut(s) 232, 265, 277
PciSI GCTCTTC 1 cut(s) 552
PkrI GCNGC 1 cut(s) 247
PshBI ATTAAT 2 cut(s) 294, 540
RsaI GTAC 1 cut(s) 561
RsaNI GTAC 1 cut(s) 560
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 2 cut(s) 294, 540
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 3 cut(s) 256, 262, 466
SetI ASST 7 cut(s) 55, 64, 79, 285, 481, 488, 561
SfaNI GCATC 1 cut(s) 212
SfuI TTCGAA 1 cut(s) 550
SmlI CTYRAG 1 cut(s) 306
SmoI CTYRAG 1 cut(s) 306
SpeI ACTAGT 1 cut(s) 122
Sse9I AATT 4 cut(s) 10, 220, 351, 537
SspMI CTAG 4 cut(s) 48, 123, 135, 165
TaqI TCGA 1 cut(s) 550
TasI AATT 4 cut(s) 10, 220, 351, 537
Tru1I TTAA 2 cut(s) 294, 540
Tru9I TTAA 2 cut(s) 294, 540
TscAI CASTG 2 cut(s) 247, 307
TseI GCWGC 1 cut(s) 245
TspDTI ATGAA 7 cut(s) 23, 87, 221, 280, 282, 392, 435
TspRI CASTG 2 cut(s) 247, 307
VspI ATTAAT 2 cut(s) 294, 540
XapI RAATTY 1 cut(s) 351
XspI CTAG 4 cut(s) 48, 123, 135, 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.