MD12G1211800.v1.1
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001357
Physical Location & Seq
Forward (+)
29071111 .. 29073604
2494 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1211800.v1.1.491

Sequence Viewer

Length: 453 bp
ATGTATTGTCAGGGTGAATCTTCTAATACTACTCACAATTCTCCAGAGTCTTCTCAATATTTCAATGGTGGTTTTGGCTTTGTTAGCGGCAGCAACACTAACTCTCAGGCTCGTGGATCAAGACCAACTGGAGGATTTGTGTCACAAGCCAATTTGCCTCAGTTTCAAGGGAATGGAACTTTTCAGCATCAAAGGAATTATCATAACAATGGTGCTAACAATGGCAGGTTCAATAACAGACCAAGGTTTAATAATGGATCTTCTGGAAGATTTTCTGAGAATTCTAATTACTTCTCTGGTAGCTCCAGTAATAATGGAGGATCTAATTTTGGGGCTAAGGGAGGATCTACTTGGCAGAATTGGAATGGTAATAATGGATCAAAGAGCGCTTTGATTCCTGAATGCCAAATTTGCAACAAAAGAGGCCACACTGCCCCAAATTGTTACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

151

Amino Acids

16.11

Weight (kDa)

9.69

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 216
AciI CCGC 1 cut(s) 87
AclWI GGATC 5 cut(s) 124, 265, 328, 352, 385
AcsI RAATTY 2 cut(s) 280, 408
AfeI AGCGCT 1 cut(s) 388
AfiI CCNNNNNNNGG 1 cut(s) 131
AgsI TTSAA 3 cut(s) 64, 167, 232
AluBI AGCT 1 cut(s) 303
AluI AGCT 1 cut(s) 303
AlwI GGATC 5 cut(s) 124, 265, 328, 352, 385
Aor51HI AGCGCT 1 cut(s) 388
AoxI GGCC 1 cut(s) 424
ApeKI GCWGC 1 cut(s) 90
ApoI RAATTY 2 cut(s) 280, 408
Asp700I GAANNNNTTC 1 cut(s) 271
AspLEI GCGC 1 cut(s) 389
AsuHPI GGTGA 1 cut(s) 26
BauI CACGAG 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 42
BbvI GCAGC 1 cut(s) 102
BfoI RGCGCY 1 cut(s) 390
BfuAI ACCTGC 1 cut(s) 216
BisI GCNGC 2 cut(s) 88, 91
BlsI GCNGC 2 cut(s) 89, 92
BmsI GCATC 1 cut(s) 196
BpiI GAAGAC 1 cut(s) 42
BpmI CTGGAG 3 cut(s) 27, 150, 289
Bpu10I CCTNAGC 1 cut(s) 336
BsaJI CCNNGG 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse1I ACTGG 2 cut(s) 133, 306
BseDI CCNNGG 1 cut(s) 242
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMII CTCAG 3 cut(s) 119, 173, 267
BseNI ACTGG 2 cut(s) 133, 306
BseXI GCAGC 1 cut(s) 102
BshFI GGCC 1 cut(s) 426
BslI CCNNNNNNNGG 1 cut(s) 131
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 1 cut(s) 426
Bsp143I GATC 5 cut(s) 116, 257, 320, 344, 377
BspACI CCGC 1 cut(s) 87
BspANI GGCC 1 cut(s) 426
BspCNI CTCAG 3 cut(s) 118, 172, 268
BspMI ACCTGC 1 cut(s) 216
BspPI GGATC 5 cut(s) 124, 265, 328, 352, 385
BsrI ACTGG 2 cut(s) 133, 306
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 5 cut(s) 116, 257, 320, 344, 377
BssSI CACGAG 1 cut(s) 111
BssT1I CCWWGG 1 cut(s) 242
Bst2BI CACGAG 1 cut(s) 111
BstDEI CTNAG 4 cut(s) 105, 159, 276, 336
BstH2I RGCGCY 1 cut(s) 390
BstHHI GCGC 1 cut(s) 389
BstKTI GATC 5 cut(s) 119, 260, 323, 347, 380
BstMBI GATC 5 cut(s) 116, 257, 320, 344, 377
BstMWI GCNNNNNNNGC 2 cut(s) 84, 411
BstV1I GCAGC 1 cut(s) 102
BstV2I GAAGAC 1 cut(s) 42
BstX2I RGATCY 3 cut(s) 257, 320, 344
BstYI RGATCY 3 cut(s) 257, 320, 344
BsuRI GGCC 1 cut(s) 426
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 1 cut(s) 429
BveI ACCTGC 1 cut(s) 216
CfoI GCGC 1 cut(s) 389
CviJI RGCY 6 cut(s) 78, 110, 149, 303, 335, 426
CviKI_1 RGCY 6 cut(s) 78, 110, 149, 303, 335, 426
DdeI CTNAG 4 cut(s) 105, 159, 276, 336
DpnI GATC 5 cut(s) 118, 259, 322, 346, 379
DpnII GATC 5 cut(s) 116, 257, 320, 344, 377
Eco130I CCWWGG 1 cut(s) 242
Eco47III AGCGCT 1 cut(s) 388
EcoRI GAATTC 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 242
ErhI CCWWGG 1 cut(s) 242
FaiI YATR 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 88, 91
Fsp4HI GCNGC 2 cut(s) 88, 91
GlaI GCGC 1 cut(s) 388
GluI GCNGC 2 cut(s) 88, 91
GsuI CTGGAG 3 cut(s) 27, 150, 289
HaeII RGCGCY 1 cut(s) 390
HaeIII GGCC 1 cut(s) 426
HhaI GCGC 1 cut(s) 389
Hin6I GCGC 1 cut(s) 387
HinP1I GCGC 1 cut(s) 387
HinfI GANTC 3 cut(s) 17, 47, 394
HphI GGTGA 1 cut(s) 26
Hpy188I TCNGA 1 cut(s) 277
Hpy188III TCNNGA 5 cut(s) 44, 120, 264, 398, 450
HpyCH4V TGCA 1 cut(s) 414
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 411
HpyF3I CTNAG 4 cut(s) 105, 159, 276, 336
HspAI GCGC 1 cut(s) 387
Kzo9I GATC 5 cut(s) 116, 257, 320, 344, 377
LmnI GCTCC 1 cut(s) 308
LpnPI CCDG 8 cut(s) 57, 92, 114, 211, 249, 282, 319, 411
Lsp1109I GCAGC 1 cut(s) 102
LweI GCATC 1 cut(s) 196
MaeIII GTNAC 2 cut(s) 141, 443
MalI GATC 5 cut(s) 118, 259, 322, 346, 379
MboI GATC 5 cut(s) 116, 257, 320, 344, 377
MboII GAAGA 4 cut(s) 12, 42, 252, 279
MflI RGATCY 3 cut(s) 257, 320, 344
MluCI AATT 9 cut(s) 37, 151, 196, 280, 286, 325, 358, 408, 439
MlyI GAGTC 1 cut(s) 56
MnlI CCTC 5 cut(s) 125, 168, 311, 335, 416
MroXI GAANNNNTTC 1 cut(s) 271
MseI TTAA 1 cut(s) 249
MslI CAYNNNNRTG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 407
MwoI GCNNNNNNNGC 2 cut(s) 84, 411
NdeII GATC 5 cut(s) 116, 257, 320, 344, 377
NmuCI GTSAC 1 cut(s) 141
PctI GAATGC 1 cut(s) 407
PdmI GAANNNNTTC 1 cut(s) 271
PfeI GAWTC 2 cut(s) 17, 394
PkrI GCNGC 2 cut(s) 89, 92
PleI GAGTC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 55
PsuI RGATCY 3 cut(s) 257, 320, 344
RseI CAYNNNNRTG 1 cut(s) 207
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 2 cut(s) 88, 91
Sau3AI GATC 5 cut(s) 116, 257, 320, 344, 377
SchI GAGTC 1 cut(s) 56
SetI ASST 3 cut(s) 230, 248, 305
SfaNI GCATC 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 207
Sse9I AATT 9 cut(s) 37, 151, 196, 280, 286, 325, 358, 408, 439
SsiI CCGC 1 cut(s) 87
SspI AATATT 1 cut(s) 59
StyI CCWWGG 1 cut(s) 242
TasI AATT 9 cut(s) 37, 151, 196, 280, 286, 325, 358, 408, 439
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 2 cut(s) 17, 394
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 1 cut(s) 141
TseI GCWGC 1 cut(s) 90
Tsp45I GTSAC 1 cut(s) 141
TspRI CASTG 1 cut(s) 436
XapI RAATTY 2 cut(s) 280, 408
XmnI GAANNNNTTC 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.