Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 94 - 459 | 9.9e-36 | Phosphofructokinase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 969 |
| Acc36I | ACCTGC | 1 cut(s) | 409 |
| AccB1I | GGYRCC | 2 cut(s) | 43, 1463 |
| AccI | GTMKAC | 1 cut(s) | 150 |
| AccII | CGCG | 3 cut(s) | 60, 194, 363 |
| AciI | CCGC | 7 cut(s) | 27, 58, 168, 171, 893, 956, 1467 |
| AclWI | GGATC | 2 cut(s) | 1166, 1179 |
| AcoI | YGGCCR | 2 cut(s) | 55, 1276 |
| AcsI | RAATTY | 3 cut(s) | 339, 1050, 1565 |
| AcuI | CTGAAG | 2 cut(s) | 1014, 1158 |
| AcyI | GRCGYC | 1 cut(s) | 44 |
| AdeI | CACNNNGTG | 1 cut(s) | 328 |
| AfaI | GTAC | 1 cut(s) | 804 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 53 |
| AjiI | CACGTC | 1 cut(s) | 1489 |
| AjnI | CCWGG | 3 cut(s) | 313, 1146, 1615 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 542, 574, 1440, 1472 |
| Alw21I | GWGCWC | 3 cut(s) | 129, 377, 1384 |
| Alw26I | GTCTC | 7 cut(s) | 42, 55, 67, 492, 533, 1116, 1492 |
| AlwI | GGATC | 2 cut(s) | 1166, 1179 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 910 |
| Ama87I | CYCGRG | 2 cut(s) | 648, 1679 |
| AoxI | GGCC | 5 cut(s) | 55, 304, 1056, 1276, 1636 |
| ApeKI | GCWGC | 5 cut(s) | 262, 821, 1373, 1433, 1550 |
| ApoI | RAATTY | 3 cut(s) | 339, 1050, 1565 |
| AspLEI | GCGC | 3 cut(s) | 32, 46, 365 |
| AspS9I | GGNCC | 7 cut(s) | 82, 155, 398, 700, 1057, 1619, 1637 |
| AsuC2I | CCSGG | 1 cut(s) | 775 |
| AsuHPI | GGTGA | 2 cut(s) | 664, 692 |
| AsuNHI | GCTAGC | 1 cut(s) | 161 |
| AvaI | CYCGRG | 2 cut(s) | 648, 1679 |
| AvaII | GGWCC | 5 cut(s) | 82, 155, 398, 700, 1619 |
| AxyI | CCTNAGG | 1 cut(s) | 1017 |
| BalI | TGGCCA | 1 cut(s) | 1278 |
| BamHI | GGATCC | 1 cut(s) | 1171 |
| BanI | GGYRCC | 2 cut(s) | 43, 1463 |
| BanII | GRGCYC | 2 cut(s) | 1384, 1686 |
| Bbv12I | GWGCWC | 3 cut(s) | 129, 377, 1384 |
| BbvI | GCAGC | 5 cut(s) | 249, 833, 1360, 1420, 1537 |
| BccI | CCATC | 4 cut(s) | 557, 671, 1073, 1477 |
| BceAI | ACGGC | 5 cut(s) | 40, 78, 121, 741, 821 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 140, 174 |
| BciT130I | CCWGG | 3 cut(s) | 315, 1148, 1617 |
| BclI | TGATCA | 1 cut(s) | 1521 |
| BcnI | CCSGG | 1 cut(s) | 775 |
| BcoDI | GTCTC | 7 cut(s) | 42, 55, 67, 492, 533, 1116, 1492 |
| BfaI | CTAG | 1 cut(s) | 162 |
| BfmI | CTRYAG | 1 cut(s) | 1115 |
| BfoI | RGCGCY | 2 cut(s) | 33, 47 |
| BfuAI | ACCTGC | 1 cut(s) | 409 |
| BisI | GCNGC | 9 cut(s) | 28, 58, 171, 263, 822, 894, 1374, 1434, 1551 |
| BlsI | GCNGC | 9 cut(s) | 29, 59, 172, 264, 823, 895, 1375, 1435, 1552 |
| Bme1390I | CCNGG | 4 cut(s) | 315, 775, 1148, 1617 |
| Bme18I | GGWCC | 5 cut(s) | 82, 155, 398, 700, 1619 |
| BmeT110I | CYCGRG | 2 cut(s) | 648, 1679 |
| BmgBI | CACGTC | 1 cut(s) | 1489 |
| BmgT120I | GGNCC | 7 cut(s) | 82, 155, 398, 700, 1057, 1619, 1637 |
| BmiI | GGNNCC | 5 cut(s) | 45, 702, 1173, 1465, 1621 |
| BmrFI | CCNGG | 4 cut(s) | 315, 775, 1148, 1617 |
| BmrI | ACTGGG | 1 cut(s) | 1413 |
| BmsI | GCATC | 4 cut(s) | 760, 1149, 1206, 1580 |
| BmtI | GCTAGC | 1 cut(s) | 165 |
| BmuI | ACTGGG | 1 cut(s) | 1413 |
| BpmI | CTGGAG | 4 cut(s) | 321, 336, 498, 1398 |
| BpuMI | CCSGG | 1 cut(s) | 775 |
| BsaHI | GRCGYC | 1 cut(s) | 44 |
| BsaI | GGTCTC | 1 cut(s) | 55 |
| BsaJI | CCNNGG | 3 cut(s) | 120, 1273, 1285 |
| BsaWI | WCCGGW | 2 cut(s) | 439, 782 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 873, 903, 1489, 1519 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 53 |
| Bse118I | RCCGGY | 2 cut(s) | 46, 173 |
| Bse1I | ACTGG | 3 cut(s) | 85, 252, 1408 |
| Bse21I | CCTNAGG | 1 cut(s) | 1017 |
| Bse3DI | GCAATG | 2 cut(s) | 763, 1539 |
| BseBI | CCWGG | 3 cut(s) | 315, 1148, 1617 |
| BseDI | CCNNGG | 3 cut(s) | 120, 1273, 1285 |
| BseGI | GGATG | 2 cut(s) | 568, 859 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 53 |
| BseMI | GCAATG | 2 cut(s) | 763, 1539 |
| BseMII | CTCAG | 2 cut(s) | 1008, 1568 |
| BseNI | ACTGG | 3 cut(s) | 85, 252, 1408 |
| BseRI | GAGGAG | 4 cut(s) | 55, 200, 597, 1510 |
| BseX3I | CGGCCG | 1 cut(s) | 55 |
| BseXI | GCAGC | 5 cut(s) | 249, 833, 1360, 1420, 1537 |
| BseYI | CCCAGC | 1 cut(s) | 1639 |
| BsgI | GTGCAG | 4 cut(s) | 276, 840, 1043, 1536 |
| Bsh1236I | CGCG | 3 cut(s) | 60, 194, 363 |
| Bsh1285I | CGRYCG | 1 cut(s) | 58 |
| BshFI | GGCC | 5 cut(s) | 57, 306, 1058, 1278, 1638 |
| BshNI | GGYRCC | 2 cut(s) | 43, 1463 |
| BsiEI | CGRYCG | 1 cut(s) | 58 |
| BsiHKAI | GWGCWC | 3 cut(s) | 129, 377, 1384 |
| BsiHKCI | CYCGRG | 2 cut(s) | 648, 1679 |
| BsiSI | CCGG | 7 cut(s) | 41, 47, 54, 174, 440, 775, 783 |
| BslFI | GGGAC | 3 cut(s) | 645, 686, 1605 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 53 |
| BsmAI | GTCTC | 7 cut(s) | 42, 55, 67, 492, 533, 1116, 1492 |
| BsmBI | CGTCTC | 2 cut(s) | 42, 67 |
| BsmFI | GGGAC | 3 cut(s) | 645, 686, 1605 |
| BsnI | GGCC | 5 cut(s) | 57, 306, 1058, 1278, 1638 |
| Bso31I | GGTCTC | 1 cut(s) | 55 |
| BsoBI | CYCGRG | 2 cut(s) | 648, 1679 |
| Bsp1286I | GDGCHC | 4 cut(s) | 129, 377, 1384, 1686 |
| Bsp143I | GATC | 4 cut(s) | 189, 358, 1171, 1521 |
| Bsp19I | CCATGG | 1 cut(s) | 1273 |
| Bsp68I | TCGCGA | 1 cut(s) | 194 |
| BspACI | CCGC | 7 cut(s) | 27, 58, 168, 171, 893, 956, 1467 |
| BspANI | GGCC | 5 cut(s) | 57, 306, 1058, 1278, 1638 |
| BspCNI | CTCAG | 2 cut(s) | 1009, 1569 |
| BspFNI | CGCG | 3 cut(s) | 60, 194, 363 |
| BspHI | TCATGA | 2 cut(s) | 411, 762 |
| BspLI | GGNNCC | 5 cut(s) | 45, 702, 1173, 1465, 1621 |
| BspMI | ACCTGC | 1 cut(s) | 409 |
| BspOI | GCTAGC | 1 cut(s) | 165 |
| BspPI | GGATC | 2 cut(s) | 1166, 1179 |
| BspT107I | GGYRCC | 2 cut(s) | 43, 1463 |
| BspTNI | GGTCTC | 1 cut(s) | 55 |
| BsrDI | GCAATG | 2 cut(s) | 763, 1539 |
| BsrFI | RCCGGY | 2 cut(s) | 46, 173 |
| BsrI | ACTGG | 3 cut(s) | 85, 252, 1408 |
| BssAI | RCCGGY | 2 cut(s) | 46, 173 |
| BssECI | CCNNGG | 3 cut(s) | 120, 1273, 1285 |
| BssMI | GATC | 4 cut(s) | 189, 358, 1171, 1521 |
| BssNI | GRCGYC | 1 cut(s) | 44 |
| BssT1I | CCWWGG | 2 cut(s) | 1273, 1285 |
| Bst2UI | CCWGG | 3 cut(s) | 315, 1148, 1617 |
| Bst4CI | ACNGT | 5 cut(s) | 155, 234, 977, 1456, 1505 |
| Bst6I | CTCTTC | 2 cut(s) | 490, 879 |
| BstACI | GRCGYC | 1 cut(s) | 44 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1159 |
| BstC8I | GCNNGC | 7 cut(s) | 163, 175, 531, 607, 730, 819, 954 |
| BstDEI | CTNAG | 3 cut(s) | 1017, 1577, 1655 |
| BstDSI | CCRYGG | 1 cut(s) | 1273 |
| BstF5I | GGATG | 2 cut(s) | 568, 859 |
| BstFNI | CGCG | 3 cut(s) | 60, 194, 363 |
| BstH2I | RGCGCY | 2 cut(s) | 33, 47 |
| BstHHI | GCGC | 3 cut(s) | 32, 46, 365 |
| BstKTI | GATC | 4 cut(s) | 192, 361, 1174, 1524 |
| BstMAI | GTCTC | 7 cut(s) | 42, 55, 67, 492, 533, 1116, 1492 |
| BstMBI | GATC | 4 cut(s) | 189, 358, 1171, 1521 |
| BstMCI | CGRYCG | 1 cut(s) | 58 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 133, 142, 167, 526, 814, 1159, 1244, 1379 |
| BstNI | CCWGG | 3 cut(s) | 315, 1148, 1617 |
| BstSCI | CCNGG | 4 cut(s) | 313, 773, 1146, 1615 |
| BstSFI | CTRYAG | 1 cut(s) | 1115 |
| BstUI | CGCG | 3 cut(s) | 60, 194, 363 |
| BstV1I | GCAGC | 5 cut(s) | 249, 833, 1360, 1420, 1537 |
| BstX2I | RGATCY | 1 cut(s) | 1171 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 314 |
| BstYI | RGATCY | 1 cut(s) | 1171 |
| BstZI | CGGCCG | 1 cut(s) | 55 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1017 |
| BsuRI | GGCC | 5 cut(s) | 57, 306, 1058, 1278, 1638 |
| BtgI | CCRYGG | 1 cut(s) | 1273 |
| BtrI | CACGTC | 1 cut(s) | 1489 |
| BtsCI | GGATG | 2 cut(s) | 568, 859 |
| BtsIMutI | CAGTG | 2 cut(s) | 259, 908 |
| BtuMI | TCGCGA | 1 cut(s) | 194 |
| BveI | ACCTGC | 1 cut(s) | 409 |
| Cac8I | GCNNGC | 7 cut(s) | 163, 175, 531, 607, 730, 819, 954 |
| CaiI | CAGNNNCTG | 1 cut(s) | 910 |
| CciI | TCATGA | 2 cut(s) | 411, 762 |
| CfoI | GCGC | 3 cut(s) | 32, 46, 365 |
| Cfr10I | RCCGGY | 2 cut(s) | 46, 173 |
| Cfr13I | GGNCC | 7 cut(s) | 82, 155, 398, 700, 1057, 1619, 1637 |
| CseI | GACGC | 2 cut(s) | 49, 1352 |
| Csp6I | GTAC | 1 cut(s) | 803 |
| CviAII | CATG | 6 cut(s) | 104, 412, 763, 1178, 1274, 1391 |
| CviQI | GTAC | 1 cut(s) | 803 |
| DdeI | CTNAG | 3 cut(s) | 1017, 1577, 1655 |
| DinI | GGCGCC | 1 cut(s) | 45 |
| DpnI | GATC | 4 cut(s) | 191, 360, 1173, 1523 |
| DpnII | GATC | 4 cut(s) | 189, 358, 1171, 1521 |
| DraIII | CACNNNGTG | 1 cut(s) | 328 |
| EaeI | YGGCCR | 2 cut(s) | 55, 1276 |
| EagI | CGGCCG | 1 cut(s) | 55 |
| Eam1104I | CTCTTC | 2 cut(s) | 490, 879 |
| EarI | CTCTTC | 2 cut(s) | 490, 879 |
| EciI | GGCGGA | 1 cut(s) | 157 |
| Ecl136II | GAGCTC | 1 cut(s) | 1382 |
| EclXI | CGGCCG | 1 cut(s) | 55 |
| Eco130I | CCWWGG | 2 cut(s) | 1273, 1285 |
| Eco24I | GRGCYC | 2 cut(s) | 1384, 1686 |
| Eco31I | GGTCTC | 1 cut(s) | 55 |
| Eco32I | GATATC | 1 cut(s) | 1413 |
| Eco47I | GGWCC | 5 cut(s) | 82, 155, 398, 700, 1619 |
| Eco52I | CGGCCG | 1 cut(s) | 55 |
| Eco53kI | GAGCTC | 1 cut(s) | 1382 |
| Eco57I | CTGAAG | 2 cut(s) | 1014, 1158 |
| Eco81I | CCTNAGG | 1 cut(s) | 1017 |
| Eco88I | CYCGRG | 2 cut(s) | 648, 1679 |
| EcoICRI | GAGCTC | 1 cut(s) | 1382 |
| EcoO109I | RGGNCCY | 1 cut(s) | 700 |
| EcoRII | CCWGG | 3 cut(s) | 313, 1146, 1615 |
| EcoRV | GATATC | 1 cut(s) | 1413 |
| EcoT14I | CCWWGG | 2 cut(s) | 1273, 1285 |
| EcoT22I | ATGCAT | 1 cut(s) | 1363 |
| EcoT38I | GRGCYC | 2 cut(s) | 1384, 1686 |
| EgeI | GGCGCC | 1 cut(s) | 45 |
| EheI | GGCGCC | 1 cut(s) | 45 |
| ErhI | CCWWGG | 2 cut(s) | 1273, 1285 |
| Esp3I | CGTCTC | 2 cut(s) | 42, 67 |
| FaeI | CATG | 6 cut(s) | 107, 415, 766, 1181, 1277, 1394 |
| FaqI | GGGAC | 3 cut(s) | 645, 686, 1605 |
| FatI | CATG | 6 cut(s) | 103, 411, 762, 1177, 1273, 1390 |
| FbaI | TGATCA | 1 cut(s) | 1521 |
| FblI | GTMKAC | 1 cut(s) | 150 |
| Fnu4HI | GCNGC | 9 cut(s) | 28, 58, 171, 263, 822, 894, 1374, 1434, 1551 |
| FokI | GGATG | 2 cut(s) | 575, 846 |
| FriOI | GRGCYC | 2 cut(s) | 1384, 1686 |
| Fsp4HI | GCNGC | 9 cut(s) | 28, 58, 171, 263, 822, 894, 1374, 1434, 1551 |
| FspBI | CTAG | 1 cut(s) | 162 |
| GlaI | GCGC | 3 cut(s) | 31, 45, 364 |
| GluI | GCNGC | 9 cut(s) | 28, 58, 171, 263, 822, 894, 1374, 1434, 1551 |
| GsaI | CCCAGC | 1 cut(s) | 1643 |
| GsuI | CTGGAG | 4 cut(s) | 321, 336, 498, 1398 |
| HaeII | RGCGCY | 2 cut(s) | 33, 47 |
| HaeIII | GGCC | 5 cut(s) | 57, 306, 1058, 1278, 1638 |
| HapII | CCGG | 7 cut(s) | 41, 47, 54, 174, 440, 775, 783 |
| HgaI | GACGC | 2 cut(s) | 49, 1352 |
| HhaI | GCGC | 3 cut(s) | 32, 46, 365 |
| Hin1I | GRCGYC | 1 cut(s) | 44 |
| Hin1II | CATG | 6 cut(s) | 107, 415, 766, 1181, 1277, 1394 |
| Hin6I | GCGC | 3 cut(s) | 30, 44, 363 |
| HinP1I | GCGC | 3 cut(s) | 30, 44, 363 |
| HincII | GTYRAC | 3 cut(s) | 151, 780, 1423 |
| HindII | GTYRAC | 3 cut(s) | 151, 780, 1423 |
| HindIII | AAGCTT | 1 cut(s) | 551 |
| HinfI | GANTC | 4 cut(s) | 4, 248, 590, 1600 |
| HpaI | GTTAAC | 1 cut(s) | 1423 |
| HpaII | CCGG | 7 cut(s) | 41, 47, 54, 174, 440, 775, 783 |
| HphI | GGTGA | 2 cut(s) | 664, 692 |
| Hpy166II | GTNNAC | 7 cut(s) | 73, 151, 398, 780, 937, 1328, 1423 |
| Hpy188I | TCNGA | 3 cut(s) | 392, 745, 1578 |
| Hpy8I | GTNNAC | 7 cut(s) | 73, 151, 398, 780, 937, 1328, 1423 |
| Hpy99I | CGWCG | 2 cut(s) | 152, 155 |
| HpyAV | CCTTC | 6 cut(s) | 234, 989, 1256, 1307, 1397, 1522 |
| HpyCH4III | ACNGT | 5 cut(s) | 155, 234, 977, 1456, 1505 |
| HpyCH4IV | ACGT | 1 cut(s) | 1488 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 133, 142, 167, 526, 814, 1159, 1244, 1379 |
| HpyF3I | CTNAG | 3 cut(s) | 1017, 1577, 1655 |
| HpySE526I | ACGT | 1 cut(s) | 1488 |
| Hsp92I | GRCGYC | 1 cut(s) | 44 |
| Hsp92II | CATG | 6 cut(s) | 107, 415, 766, 1181, 1277, 1394 |
| HspAI | GCGC | 3 cut(s) | 30, 44, 363 |
| KasI | GGCGCC | 1 cut(s) | 43 |
| KroI | GCCGGC | 1 cut(s) | 173 |
| KroNI | GCCGGC | 1 cut(s) | 175 |
| Ksp22I | TGATCA | 1 cut(s) | 1521 |
| KspAI | GTTAAC | 1 cut(s) | 1423 |
| Kzo9I | GATC | 4 cut(s) | 189, 358, 1171, 1521 |
| LmnI | GCTCC | 5 cut(s) | 170, 372, 1150, 1379, 1441 |
| Lsp1109I | GCAGC | 5 cut(s) | 249, 833, 1360, 1420, 1537 |
| LweI | GCATC | 4 cut(s) | 760, 1149, 1206, 1580 |
| MaeI | CTAG | 1 cut(s) | 162 |
| MaeII | ACGT | 1 cut(s) | 1488 |
| MaeIII | GTNAC | 4 cut(s) | 320, 919, 1604, 1658 |
| MalI | GATC | 4 cut(s) | 191, 360, 1173, 1523 |
| MboI | GATC | 4 cut(s) | 189, 358, 1171, 1521 |
| MboII | GAAGA | 8 cut(s) | 219, 292, 507, 896, 1088, 1225, 1329, 1457 |
| MflI | RGATCY | 1 cut(s) | 1171 |
| MhlI | GDGCHC | 4 cut(s) | 129, 377, 1384, 1686 |
| MlsI | TGGCCA | 1 cut(s) | 1278 |
| MluNI | TGGCCA | 1 cut(s) | 1278 |
| Mly113I | GGCGCC | 1 cut(s) | 44 |
| MlyI | GAGTC | 2 cut(s) | 13, 584 |
| Mox20I | TGGCCA | 1 cut(s) | 1278 |
| Mph1103I | ATGCAT | 1 cut(s) | 1363 |
| MroNI | GCCGGC | 1 cut(s) | 173 |
| MscI | TGGCCA | 1 cut(s) | 1278 |
| MseI | TTAA | 4 cut(s) | 525, 627, 1422, 1693 |
| MslI | CAYNNNNRTG | 2 cut(s) | 841, 1182 |
| Msp20I | TGGCCA | 1 cut(s) | 1278 |
| MspA1I | CMGCKG | 1 cut(s) | 545 |
| MspI | CCGG | 7 cut(s) | 41, 47, 54, 174, 440, 775, 783 |
| MspR9I | CCNGG | 4 cut(s) | 315, 775, 1148, 1617 |
| MvaI | CCWGG | 3 cut(s) | 315, 1148, 1617 |
| MvnI | CGCG | 3 cut(s) | 60, 194, 363 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 133, 142, 167, 526, 814, 1159, 1244, 1379 |
| NaeI | GCCGGC | 1 cut(s) | 175 |
| NarI | GGCGCC | 1 cut(s) | 44 |
| NciI | CCSGG | 1 cut(s) | 775 |
| NcoI | CCATGG | 1 cut(s) | 1273 |
| NdeII | GATC | 4 cut(s) | 189, 358, 1171, 1521 |
| NgoMIV | GCCGGC | 1 cut(s) | 173 |
| NheI | GCTAGC | 1 cut(s) | 161 |
| NlaIII | CATG | 6 cut(s) | 107, 415, 766, 1181, 1277, 1394 |
| NlaIV | GGNNCC | 5 cut(s) | 45, 702, 1173, 1465, 1621 |
| NmuCI | GTSAC | 3 cut(s) | 320, 919, 1658 |
| NruI | TCGCGA | 1 cut(s) | 194 |
| NsiI | ATGCAT | 1 cut(s) | 1363 |
| PagI | TCATGA | 2 cut(s) | 411, 762 |
| PdiI | GCCGGC | 1 cut(s) | 175 |
| PfeI | GAWTC | 2 cut(s) | 248, 1600 |
| PfoI | TCCNGGA | 1 cut(s) | 1146 |
| PkrI | GCNGC | 9 cut(s) | 29, 59, 172, 264, 823, 895, 1375, 1435, 1552 |
| PleI | GAGTC | 2 cut(s) | 12, 584 |
| PluTI | GGCGCC | 1 cut(s) | 47 |
| PpsI | GAGTC | 2 cut(s) | 12, 584 |
| PpuMI | RGGWCCY | 1 cut(s) | 700 |
| PsiI | TTATAA | 1 cut(s) | 969 |
| Psp124BI | GAGCTC | 1 cut(s) | 1384 |
| Psp5II | RGGWCCY | 1 cut(s) | 700 |
| Psp6I | CCWGG | 3 cut(s) | 313, 1146, 1615 |
| PspFI | CCCAGC | 1 cut(s) | 1639 |
| PspGI | CCWGG | 3 cut(s) | 313, 1146, 1615 |
| PspN4I | GGNNCC | 5 cut(s) | 45, 702, 1173, 1465, 1621 |
| PspPI | GGNCC | 7 cut(s) | 82, 155, 398, 700, 1057, 1619, 1637 |
| PspPPI | RGGWCCY | 1 cut(s) | 700 |
| PstNI | CAGNNNCTG | 1 cut(s) | 910 |
| PsuI | RGATCY | 1 cut(s) | 1171 |
| PvuII | CAGCTG | 1 cut(s) | 545 |
| RruI | TCGCGA | 1 cut(s) | 194 |
| RsaI | GTAC | 1 cut(s) | 804 |
| RsaNI | GTAC | 1 cut(s) | 803 |
| RseI | CAYNNNNRTG | 2 cut(s) | 841, 1182 |
| SacI | GAGCTC | 1 cut(s) | 1384 |
| SalI | GTCGAC | 1 cut(s) | 149 |
| SaqAI | TTAA | 4 cut(s) | 525, 627, 1422, 1693 |
| SatI | GCNGC | 9 cut(s) | 28, 58, 171, 263, 822, 894, 1374, 1434, 1551 |
| Sau3AI | GATC | 4 cut(s) | 189, 358, 1171, 1521 |
| Sau96I | GGNCC | 7 cut(s) | 82, 155, 398, 700, 1057, 1619, 1637 |
| SchI | GAGTC | 2 cut(s) | 13, 584 |
| ScrFI | CCNGG | 4 cut(s) | 315, 775, 1148, 1617 |
| SduI | GDGCHC | 4 cut(s) | 129, 377, 1384, 1686 |
| SfaNI | GCATC | 4 cut(s) | 760, 1149, 1206, 1580 |
| SfcI | CTRYAG | 1 cut(s) | 1115 |
| SfoI | GGCGCC | 1 cut(s) | 45 |
| SgrDI | CGTCGACG | 1 cut(s) | 149 |
| SinI | GGWCC | 5 cut(s) | 82, 155, 398, 700, 1619 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 841, 1182 |
| SsiI | CCGC | 7 cut(s) | 27, 58, 168, 171, 893, 956, 1467 |
| SspDI | GGCGCC | 1 cut(s) | 43 |
| SspI | AATATT | 1 cut(s) | 791 |
| SspMI | CTAG | 1 cut(s) | 162 |
| SstI | GAGCTC | 1 cut(s) | 1384 |
| StyD4I | CCNGG | 4 cut(s) | 313, 773, 1146, 1615 |
| StyI | CCWWGG | 2 cut(s) | 1273, 1285 |
| TaaI | ACNGT | 5 cut(s) | 155, 234, 977, 1456, 1505 |
| TaiI | ACGT | 1 cut(s) | 1491 |
| TaqI | TCGA | 5 cut(s) | 89, 99, 150, 1164, 1686 |
| TaqII | GACCGA | 1 cut(s) | 206 |
| TauI | GCSGC | 4 cut(s) | 30, 60, 173, 896 |
| TfiI | GAWTC | 2 cut(s) | 248, 1600 |
| Tru1I | TTAA | 4 cut(s) | 525, 627, 1422, 1693 |
| Tru9I | TTAA | 4 cut(s) | 525, 627, 1422, 1693 |
| TscAI | CASTG | 2 cut(s) | 259, 915 |
| TseFI | GTSAC | 3 cut(s) | 320, 919, 1658 |
| TseI | GCWGC | 5 cut(s) | 262, 821, 1373, 1433, 1550 |
| Tsp45I | GTSAC | 3 cut(s) | 320, 919, 1658 |
| TspDTI | ATGAA | 7 cut(s) | 428, 1000, 1062, 1089, 1326, 1379, 1467 |
| TspGWI | ACGGA | 1 cut(s) | 58 |
| TspRI | CASTG | 2 cut(s) | 259, 915 |
| VpaK11BI | GGWCC | 5 cut(s) | 82, 155, 398, 700, 1619 |
| XapI | RAATTY | 3 cut(s) | 339, 1050, 1565 |
| XmiI | GTMKAC | 1 cut(s) | 150 |
| XspI | CTAG | 1 cut(s) | 162 |
| Zsp2I | ATGCAT | 1 cut(s) | 1363 |